CN109197786A - A kind of method of interior mass rearing semi-closure initial bow - Google Patents

A kind of method of interior mass rearing semi-closure initial bow Download PDF

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Publication number
CN109197786A
CN109197786A CN201811289326.5A CN201811289326A CN109197786A CN 109197786 A CN109197786 A CN 109197786A CN 201811289326 A CN201811289326 A CN 201811289326A CN 109197786 A CN109197786 A CN 109197786A
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semi
parasitic
diamondback moth
bee
cage
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CN109197786B (en
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贾变桃
安颢敏
卢晶晶
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Shanxi Agricultural University
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Shanxi Agricultural University
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/033Rearing or breeding invertebrates; New breeds of invertebrates
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for
    • A01G22/15Leaf crops, e.g. lettuce or spinach 
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/163Sugars; Polysaccharides
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/90Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Animal Husbandry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Environmental Sciences (AREA)
  • Botany (AREA)
  • Biotechnology (AREA)
  • Health & Medical Sciences (AREA)
  • Birds (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Physiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Insects & Arthropods (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Feed For Specific Animals (AREA)

Abstract

The present invention relates to a kind of methods of indoor mass rearing semi-closure initial bow, the specific steps of which are as follows: the plantation of (1) diamondback moth host plant wild cabbage: wild cabbage is transplanted to field planting after nursery according to a conventional method;(2) the indoor raising of diamondback moth: the pickles chrysalis that will sprout wings is placed in oviposition cage, and it is Adult supplement nutrient that cotton ball, which is dipped 10% sucrose water, and puts the cabbage leaves of a 2-2.5 mm thickness by its oviposition;(3) in semi-closure initial bow room parasitic environment building: take parasitic wasp, queen bee is 10, is that 1:1-1.2 chooses Xiong Feng according to sex ration, is put in parasitic cage;(4) parasitism of 3 instar larvae of diamondback moth and raising: the selection and rejuvenation of (5) kind bee: kind bee selection figure is larger and very active at bee, periodically carries out rejuvenation.The method of indoor mass rearing semi-closure initial bow of the invention, obtains a large amount of semi-closure initial bows, the needs of to meet scientific research and Field information.

Description

A kind of method of interior mass rearing semi-closure initial bow
Technical field
The present invention relates to a kind of indoor propagation methods of insect, and in particular to a kind of interior mass rearing semi-closure initial bow Method.
Background technique
Diamondback moth category lepidoptera diamond-back moth section also known as square moth are a kind of oligophagy pests, are to post with crucifer It is main, it has now been found that more than 40 kinds of its host plant, most to like cause harm wild cabbage, cauliflower and rape, followed by radish, Chinese cabbage etc..Diamondback moth Harm is serious and prevention and treatment is difficult, relies primarily on chemical prevention at present, but diamondback moth is extremely easy to produce drug resistance to insecticide.
Semi-closure initial bow is a kind of parasitic wasp of diamondback moth larvae phase, originating from Europe, is drawn by many countries and regions Into for preventing and treating diamondback moth.The thirties in last century, New Zealand introduce honeybee kind by Britain, and bee colony adapts to local environment quickly, substantially On control the harm of local diamondback moth, semi-closure initial bow may be up to 70% or more to the field nature parasitic rate of diamondback moth, quilt It is considered effectively to control the Natural Enemies of diamondback moth.But the raising of the current bee is also relatively difficult, be not able to satisfy desk research and The needs of Field information.
Summary of the invention
For above situation, the object of the present invention is to provide a kind of methods of indoor mass rearing semi-closure initial bow, obtain A large amount of semi-closure initial bows, the needs of to meet scientific research and Field information semi-closure initial bow.
A kind of method of indoor mass rearing semi-closure initial bow of the invention, the specific steps of which are as follows:
(1) plantation of diamondback moth host plant wild cabbage: wild cabbage is transplanted to field planting after nursery according to a conventional method, and winter need to be big The interior plantation of canopy, when wild cabbage grows to 10 leaves or more, picking blade is used for the raising of diamondback moth, during wild cabbage is planted and is harvested not Spray any medicament;
(2) the indoor raising of diamondback moth: the pickles chrysalis that will sprout wings is placed in oviposition cage, cotton ball is dipped into 10% sucrose Water is Adult supplement nutrient, and the cabbage leaves for putting a 2-2.5 mm thickness are replaced fresh blade daily and contained by its oviposition Ovum leaf is placed in 15 cm of diameter, in high 5 cm polyethylene round plastic box, black cloth covers until no longer lay eggs by the cotton ball of syrup On, rubber band tightens, and plastic casing is put into 25 DEG C of temperature, humidity is 70% or so, is carried out in the incubator of 16: 8 h of photoperiod Culture spreads a fresh cabbage leaves in plastic box bottom two days later and eats for larva, checks plastic casing daily, add as needed Add fresh blade, it is mouldy, eat surplus, dry blade and throw away, the larva above blade is chosen into fresh leaf on piece;
(3) in semi-closure initial bow room parasitic environment building: take parasitic wasp, queen bee is 10, is 1 ︰ 1-1.2 according to sex ration Xiong Feng is chosen, is put in parasitic cage, is supplemented the nutrients with the syrup of 10%-20% for it;
(4) parasitism of 3 instar larvae of diamondback moth is with raising: with 2 250 mL beakers or plastic cup, putting a cabbage leaves in every glass Piece accesses 200 or so 3 instar larvaes of diamondback moth, is put into the parasitic cage of step (3) for its parasitism, takes out after 1 day, will be parasitic Larva choose into clean plastic cup, every glass is no more than 50, and the fresh cabbage leaves of supplement, tighten rim of a cup with black cloth and be placed in cup It is raised in 22 DEG C of constant temperature illumination boxs;
(5) plant the selection and rejuvenation of bee: kind bee selection figure is larger and very active at bee, and periodically from field, acquisition semi-closure is curved Tail ichneumon wasp carries out rejuvenation to the bee colony of indoor raising.
Preferably, 10 cm of length, wide by 10 of oviposition 15 cm of length of cage, wide 10 cm, parasitism cage described in high 20 cm Cm, high 10 cm.
Preferably, the cabbage field area is depending on raising scale, with the supply of full feeding diamondback moth whole year.
The method of indoor mass rearing semi-closure initial bow of the invention, by raising and semi-closure initial bow in diamondback moth room Indoor propagation organically combines, using parasitic cage by parasitic wasp parasitism, to achieve the purpose that obtain mass propagation semi-closure initial bow. Key of the invention is the parasitism of 3 instar larvae of diamondback moth, guarantees to maintain 10 queen bees in each parasitic cage daily, guarantee daily There is consistent 3 instar larvae of the diamondback moth supply of the development of sufficient health not select in diamondback moth raising and parasitic processes Common Turnip Sprouts, Brassica campestris L seedling or cabbage plant are directly raised, and are selected in beaker vessel and are directly raised with cabbage leaves, Avoid diamondback moth larvae pierce in vermiculite or soil or seedling-raising box outside, be difficult to find, while facilitating operation, it is sub that every cephalont can be grasped Developmental stage, convenient for the collection of parasitic bee pupa.Ovum is put into plastic casing in step 2 and is cultivated, black cloth is coverd with, and rubber band is pricked Tightly, it can avoid Eggs of Diamondback Moth around in insect cage or incubator after hatching as larva to climb, pickles raised in insect box Moth can grasp diamondback moth developmental stage at any time, parasitism is used for conducive to suitable 3 instar larvae is finally selected, in addition, the curved tail Ji of semi-closure Bee imago needs to supplement the nutrients immediately after sprouting wings, if adult can die in 1-2 days not to supplementing the nutrients, supplements the nutrients, some The syrup that can even survive 20 days or so at bee, therefore supplement 10%-20% in step (3) is to supplement the nutrients at bee.Using The parasitic efficiency of method semi-closure initial bow of the invention is very high, general parasitic 6 hours, parasitic rate can reach 80% with On, the parasitic time is 1 day in parasitic cage in step (4), parasitic overlong time is avoided, there is hyperparasitism, influences survival rate, Long-term breeding has the population deterioration imagination to semi-closure initial bow indoors, and reproductive capacity and viability can decline, therefore, in field Diamondback moth will acquire honeybee kind when occurring from field and introduce indoor population, carry out rejuvenation to indoor population to guarantee semi-closure initial bow Reproductive capacity and viability.
Specific embodiment
With reference to embodiment, technical solution of the present invention is described in more detail;
A kind of method of interior mass rearing semi-closure initial bow, the specific steps of which are as follows:
One, working environment
Wild cabbage plants field: spring and summer can plant wild cabbage in outdoor cabbage field, and autumn and winter wild cabbage is planted in greenhouse, it is ensured that whole year has Wild cabbage supply;
Diamondback moth Reproduction Conditions: in illumination box or insectary, raising temperature is 25 DEG C or so;
Semi-closure initial bow Reproduction Conditions: in illumination box or insectary, raising temperature is 22 DEG C or so;
Diamondback moth adult mate and oviposit cage: oviposition cage size is long 15 cm, wide 10 cm, high 20 cm wooden frame, bottom plank Closing, above, two sides are closed with the stainless steel cloth of the mm of 0.5 mm × 0.5, and front is closed with velcro, and hair side is fixed on On wooden frame, hook surface is fixed on the surrounding of stainless (steel) wire, seals after fitting, and an osculum is torn from below and is operated;
Parasitic cage: size is the wooden frame of long 10 cm, wide 10 cm, high 10 cm, and surrounding diamondback moth is as mate and oviposit cage It is closed with stainless steel gauze, opening is closed with velcro;
Sucrose water: the sucrose water that configuration concentration is 10% is for being diamondback moth adult and semi-closure initial bow Adult supplement nutrient;
Two, head cabbage varieties: the rich series in capital.
The plantation of wild cabbage
Cabbage seedling is cultivated indoors or in protecting field, is transplanted to crop field or greenhouse later, regular watering, Common Cultivation are needed after transplanting Period does not spray any pesticide, such as damaged by vermin, can carry out artificial capture method.
Three, the raising of diamondback moth
1. receiving ovum
Ovum is received from oviposition cage daily, there was only ovum on two days received cabbage leaves of head, is put in plastic casing, black cloth is coverd with, rubber Muscle is put in 25 DEG C of incubators after tightening.
2. larva raising
Ovum is hatched after general 3 days, to be spread a fresh blade in plastic box bottom two days later, checked plastic casing daily, if Blade is dry or blade is eaten to be not enough to and continues to 1 day appetite of diamondback moth in box, it is necessary to fresh blade is added, it is fresh Blade will be put in plastic box bottom, it is mouldy, eat surplus, dry blade and throw away, larva above is chosen into fresh blade On;
Plastic casing is replaced according to the actual situation, and if humidity is excessive in compartmentalized box for holding assorted fruits and candies, faecal contamination is serious, can replace daily, such as compartmentalized box for holding assorted fruits and candies Interior larva is excessive, can also be changed without, and the worm time is chosen in saving;
Plastic casing is only used for the raising of 1-3 instar larvae, and diamondback moth grew to for 4 ages, it is necessary to and it chooses in plastic cup and raises, Zhi Daohua Pupa is collected after pupa, is used for follow-on raising;
In parasitic wasp parasitism, required larva is 3 instar larvaes, and the main purpose of larva raising is that acquisition development is neat, standard is consistent 3 instar larvaes, after larva reached for 3 ages, if excessively, the same day with not coming, can first be put in 4 DEG C of refrigerators, it is fast to slow down its development Degree.
3. the collection of pupa
4 instar larvaes being put in plastic cup can replace primary fresh cabbage leaves for 2 days, and in replacement process, some larvas may Prepupa pupates, therefore can be collected into plastic culture dish, writes the date with marking pen in ware lid, if the pupa being collected into It is not eager that it is allowed to sprout wings, can be placed in 4 DEG C of refrigerators and save, date saved is no more than 1 month, otherwise seriously affects its oviposition.
4. the emergence and oviposition of adult
The pickles chrysalis saved in refrigerator is put into insect cage, one big culture dish or disk are put in the inside, are put with small culture dish One fritter contains the cotton ball of 10% syrup for Adult supplement nutrient, and puts one with a thickness of 2-2.5 mm cabbage leaves by its production Ovum replaces fresh blade and cotton ball daily, until no longer laying eggs.
Four, semi-closure initial bow indoor propagation technology
Parasitic wasp oviposition completes ovum and larval phase in diamondback moth larvae body in diamondback moth larvae body;
1. the oviposition of parasitic wasp
Parasitic wasp 10 is right, and sex ration is that 1:1 is put in parasitic cage, is supplemented the nutrients with syrup for it, with 2 250 mL beakers or greatly A cabbage leaves are put in plastic cup, the inside, and 200 or so 3 instar larvaes of diamondback moth of every glass of access are put into parasitic cage and supply it parasitic, and 1 It takes out, is replaced into clean plastic cup after it, every glass is no more than 50 larvas, supplements fresh blade, tightens rim of a cup with black cloth It raises, the diamondback moth in cage is chosen in cup with tweezers.
2. receiving pupa
The same diamondback moth of method is replaced primary fresh blade in parasitic diamondback moth every two days in beaker or plastic cup, and will be pre- Pupa is chosen into plastic culture dish, if there is diamondback moth pupates, chooses in the culture dish of pickles chrysalis, and parasitic wasp pupates generally It pupates than diamondback moth late, therefore diamondback moth prepupa generally just will become parasitic bee pupa after 2 days, needs to choose pupa into parasitic wasp at this time In the culture dish of pupa.
Bee pupa can be put into 4 DEG C of refrigerators and save if not urgently needed, the holding time preferably no more than 20 days, otherwise eclosion rate meeting Decline.
3. the emergence of parasitic wasp
The parasitic wasp being stored in refrigerator is put into small insect cage, makes its emergence under the conditions of 22 DEG C, and providing syrup is it It supplements the nutrients.
4. the selection and rejuvenation of kind of bee: kind bee selection figure is larger and very active at bee, occurs in field diamondback moth When from field acquisition honeybee kind introduce indoor population, rejuvenation is carried out to indoor population.
As can be seen from the above table, parasitic ratio is parasitic 40 3 instar larvaes of diamondback moth of every queen bee, and parasitic rate is higher, posts Raw rate reaches 90.52%.
As can be seen from the above table, the parasitic rate of 3 instar larvae of semi-closure initial bow parasitism diamondback moth reaches 80% or more, parasitism Rate is high.
Table 3 is after semi-closure initial bow is 6 hours parasitic, and parasitic rate can reach 80% or more.

Claims (3)

1. a kind of method of interior mass rearing semi-closure initial bow, it is characterised in that include the next steps:
(1) plantation of diamondback moth host plant wild cabbage: wild cabbage is transplanted to field planting after nursery according to a conventional method, and winter need to be big The interior plantation of canopy, when wild cabbage grows to 10 leaves or more, picking blade is used for the raising of diamondback moth, during wild cabbage is planted and is harvested not Spray any medicament;
(2) the indoor raising of diamondback moth: the pickles chrysalis that will sprout wings is placed in oviposition cage, cotton ball is dipped into 10% sucrose Water is Adult supplement nutrient, and the cabbage leaves for putting a 2-2.5 mm thickness are replaced fresh blade daily and contained by its oviposition Ovum leaf is placed in 15 cm of diameter, in high 5 cm polyethylene round plastic box, black cloth covers until no longer lay eggs by the cotton ball of syrup On, rubber band tightens, and plastic casing is put into 25 DEG C of temperature, humidity is 70% or so, is carried out in the incubator of 16: 8 h of photoperiod Culture spreads a fresh cabbage leaves in plastic box bottom two days later and eats for larva, checks plastic casing daily, add as needed Add fresh blade, it is mouldy, eat surplus, dry blade and throw away, the larva above blade is chosen into fresh leaf on piece;
(3) in semi-closure initial bow room parasitic environment building: take parasitic wasp, queen bee is 10, is 1 ︰ 1-1.2 according to sex ration Xiong Feng is chosen, is put in parasitic cage, is supplemented the nutrients with the syrup of 10%-20% for it;
(4) parasitism of 3 instar larvae of diamondback moth is with raising: with 2 250 mL beakers or plastic cup, putting a cabbage leaves in every glass Piece accesses 200 or so 3 instar larvaes of diamondback moth, is put into the parasitic cage of step (3) for its parasitism, takes out after 1 day, will be parasitic Larva choose into clean plastic cup, every glass is no more than 50, and the fresh cabbage leaves of supplement, tighten rim of a cup with black cloth and be placed in cup It is raised in 22 DEG C of constant temperature illumination boxs;
(5) plant the selection and rejuvenation of bee: kind bee selection figure is larger and very active at bee, and periodically from field, acquisition semi-closure is curved Tail ichneumon wasp carries out rejuvenation to the bee colony of indoor raising.
2. the method for interior mass rearing semi-closure initial bow according to claim 1, it is characterised in that the oviposition cage 15 cm of length, wide 10 cm, parasitism cage described in high 20 cm 10 cm of length, wide 10 cm, high 10 cm.
3. the method for interior mass rearing semi-closure initial bow according to claim 1, it is characterised in that the cabbage field Area is depending on raising scale.
CN201811289326.5A 2018-10-31 2018-10-31 Method for breeding large amount of semi-closed curvicauda curvularia indoors Active CN109197786B (en)

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Cited By (1)

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Publication number Priority date Publication date Assignee Title
CN111034684A (en) * 2019-12-23 2020-04-21 云南省农业科学院农业环境资源研究所 Semitrella semipenguii seed conservation and rejuvenation breeding method

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Publication number Priority date Publication date Assignee Title
CN111034684A (en) * 2019-12-23 2020-04-21 云南省农业科学院农业环境资源研究所 Semitrella semipenguii seed conservation and rejuvenation breeding method

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