CN109197786A - A kind of method of interior mass rearing semi-closure initial bow - Google Patents
A kind of method of interior mass rearing semi-closure initial bow Download PDFInfo
- Publication number
- CN109197786A CN109197786A CN201811289326.5A CN201811289326A CN109197786A CN 109197786 A CN109197786 A CN 109197786A CN 201811289326 A CN201811289326 A CN 201811289326A CN 109197786 A CN109197786 A CN 109197786A
- Authority
- CN
- China
- Prior art keywords
- semi
- parasitic
- diamondback moth
- bee
- cage
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 title claims abstract description 19
- 230000000384 rearing effect Effects 0.000 title claims abstract description 12
- 241000500437 Plutella xylostella Species 0.000 claims abstract description 50
- 230000003071 parasitic effect Effects 0.000 claims abstract description 46
- 235000001169 Brassica oleracea var oleracea Nutrition 0.000 claims abstract description 29
- 240000007124 Brassica oleracea Species 0.000 claims abstract description 19
- 235000003899 Brassica oleracea var acephala Nutrition 0.000 claims abstract description 18
- 235000011301 Brassica oleracea var capitata Nutrition 0.000 claims abstract description 18
- 241001674939 Caulanthus Species 0.000 claims abstract description 16
- 230000017448 oviposition Effects 0.000 claims abstract description 14
- 235000015097 nutrients Nutrition 0.000 claims abstract description 13
- 230000024241 parasitism Effects 0.000 claims abstract description 13
- 239000013589 supplement Substances 0.000 claims abstract description 13
- 235000006008 Brassica napus var napus Nutrition 0.000 claims abstract description 11
- 230000003716 rejuvenation Effects 0.000 claims abstract description 9
- 240000001624 Espostoa lanata Species 0.000 claims abstract description 7
- 235000009161 Espostoa lanata Nutrition 0.000 claims abstract description 7
- 235000021110 pickles Nutrition 0.000 claims abstract description 6
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims abstract description 5
- 229930006000 Sucrose Natural products 0.000 claims abstract description 5
- 239000005720 sucrose Substances 0.000 claims abstract description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 5
- 238000007796 conventional method Methods 0.000 claims abstract description 3
- 239000004033 plastic Substances 0.000 claims description 26
- 229920003023 plastic Polymers 0.000 claims description 26
- 102000002322 Egg Proteins Human genes 0.000 claims description 9
- 108010000912 Egg Proteins Proteins 0.000 claims description 9
- 210000004681 ovum Anatomy 0.000 claims description 9
- 239000004744 fabric Substances 0.000 claims description 8
- 239000006188 syrup Substances 0.000 claims description 8
- 235000020357 syrup Nutrition 0.000 claims description 8
- 239000011521 glass Substances 0.000 claims description 6
- 235000013601 eggs Nutrition 0.000 claims description 4
- 238000005286 illumination Methods 0.000 claims description 4
- 241000196324 Embryophyta Species 0.000 claims description 3
- 239000007921 spray Substances 0.000 claims description 3
- 241001371826 Ichneumon suspiciosus Species 0.000 claims description 2
- 239000004698 Polyethylene Substances 0.000 claims description 2
- 239000003814 drug Substances 0.000 claims description 2
- -1 polyethylene Polymers 0.000 claims description 2
- 229920000573 polyethylene Polymers 0.000 claims description 2
- 241000256844 Apis mellifera Species 0.000 description 20
- 241000382353 Pupa Species 0.000 description 10
- 241000238631 Hexapoda Species 0.000 description 5
- 230000018109 developmental process Effects 0.000 description 3
- 235000009508 confectionery Nutrition 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- 230000002265 prevention Effects 0.000 description 2
- 230000001850 reproductive effect Effects 0.000 description 2
- 229910001220 stainless steel Inorganic materials 0.000 description 2
- 239000010935 stainless steel Substances 0.000 description 2
- 230000035899 viability Effects 0.000 description 2
- 235000005637 Brassica campestris Nutrition 0.000 description 1
- 235000011293 Brassica napus Nutrition 0.000 description 1
- 235000011299 Brassica oleracea var botrytis Nutrition 0.000 description 1
- 240000003259 Brassica oleracea var. botrytis Species 0.000 description 1
- 244000178937 Brassica oleracea var. capitata Species 0.000 description 1
- 240000008100 Brassica rapa Species 0.000 description 1
- 235000010149 Brassica rapa subsp chinensis Nutrition 0.000 description 1
- 235000000536 Brassica rapa subsp pekinensis Nutrition 0.000 description 1
- 235000000540 Brassica rapa subsp rapa Nutrition 0.000 description 1
- 241001301148 Brassica rapa subsp. oleifera Species 0.000 description 1
- 241000499436 Brassica rapa subsp. pekinensis Species 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 241000257303 Hymenoptera Species 0.000 description 1
- 244000088415 Raphanus sativus Species 0.000 description 1
- 235000006140 Raphanus sativus var sativus Nutrition 0.000 description 1
- 229910000831 Steel Inorganic materials 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 230000036528 appetite Effects 0.000 description 1
- 235000019789 appetite Nutrition 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 230000032669 eclosion Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000012447 hatching Effects 0.000 description 1
- 239000002917 insecticide Substances 0.000 description 1
- 230000001418 larval effect Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000010959 steel Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 229910052902 vermiculite Inorganic materials 0.000 description 1
- 235000019354 vermiculite Nutrition 0.000 description 1
- 239000010455 vermiculite Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/15—Leaf crops, e.g. lettuce or spinach
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/90—Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Environmental Sciences (AREA)
- Botany (AREA)
- Biotechnology (AREA)
- Health & Medical Sciences (AREA)
- Birds (AREA)
- Molecular Biology (AREA)
- Mycology (AREA)
- Physiology (AREA)
- Animal Behavior & Ethology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Insects & Arthropods (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Feed For Specific Animals (AREA)
Abstract
The present invention relates to a kind of methods of indoor mass rearing semi-closure initial bow, the specific steps of which are as follows: the plantation of (1) diamondback moth host plant wild cabbage: wild cabbage is transplanted to field planting after nursery according to a conventional method;(2) the indoor raising of diamondback moth: the pickles chrysalis that will sprout wings is placed in oviposition cage, and it is Adult supplement nutrient that cotton ball, which is dipped 10% sucrose water, and puts the cabbage leaves of a 2-2.5 mm thickness by its oviposition;(3) in semi-closure initial bow room parasitic environment building: take parasitic wasp, queen bee is 10, is that 1:1-1.2 chooses Xiong Feng according to sex ration, is put in parasitic cage;(4) parasitism of 3 instar larvae of diamondback moth and raising: the selection and rejuvenation of (5) kind bee: kind bee selection figure is larger and very active at bee, periodically carries out rejuvenation.The method of indoor mass rearing semi-closure initial bow of the invention, obtains a large amount of semi-closure initial bows, the needs of to meet scientific research and Field information.
Description
Technical field
The present invention relates to a kind of indoor propagation methods of insect, and in particular to a kind of interior mass rearing semi-closure initial bow
Method.
Background technique
Diamondback moth category lepidoptera diamond-back moth section also known as square moth are a kind of oligophagy pests, are to post with crucifer
It is main, it has now been found that more than 40 kinds of its host plant, most to like cause harm wild cabbage, cauliflower and rape, followed by radish, Chinese cabbage etc..Diamondback moth
Harm is serious and prevention and treatment is difficult, relies primarily on chemical prevention at present, but diamondback moth is extremely easy to produce drug resistance to insecticide.
Semi-closure initial bow is a kind of parasitic wasp of diamondback moth larvae phase, originating from Europe, is drawn by many countries and regions
Into for preventing and treating diamondback moth.The thirties in last century, New Zealand introduce honeybee kind by Britain, and bee colony adapts to local environment quickly, substantially
On control the harm of local diamondback moth, semi-closure initial bow may be up to 70% or more to the field nature parasitic rate of diamondback moth, quilt
It is considered effectively to control the Natural Enemies of diamondback moth.But the raising of the current bee is also relatively difficult, be not able to satisfy desk research and
The needs of Field information.
Summary of the invention
For above situation, the object of the present invention is to provide a kind of methods of indoor mass rearing semi-closure initial bow, obtain
A large amount of semi-closure initial bows, the needs of to meet scientific research and Field information semi-closure initial bow.
A kind of method of indoor mass rearing semi-closure initial bow of the invention, the specific steps of which are as follows:
(1) plantation of diamondback moth host plant wild cabbage: wild cabbage is transplanted to field planting after nursery according to a conventional method, and winter need to be big
The interior plantation of canopy, when wild cabbage grows to 10 leaves or more, picking blade is used for the raising of diamondback moth, during wild cabbage is planted and is harvested not
Spray any medicament;
(2) the indoor raising of diamondback moth: the pickles chrysalis that will sprout wings is placed in oviposition cage, cotton ball is dipped into 10% sucrose
Water is Adult supplement nutrient, and the cabbage leaves for putting a 2-2.5 mm thickness are replaced fresh blade daily and contained by its oviposition
Ovum leaf is placed in 15 cm of diameter, in high 5 cm polyethylene round plastic box, black cloth covers until no longer lay eggs by the cotton ball of syrup
On, rubber band tightens, and plastic casing is put into 25 DEG C of temperature, humidity is 70% or so, is carried out in the incubator of 16: 8 h of photoperiod
Culture spreads a fresh cabbage leaves in plastic box bottom two days later and eats for larva, checks plastic casing daily, add as needed
Add fresh blade, it is mouldy, eat surplus, dry blade and throw away, the larva above blade is chosen into fresh leaf on piece;
(3) in semi-closure initial bow room parasitic environment building: take parasitic wasp, queen bee is 10, is 1 ︰ 1-1.2 according to sex ration
Xiong Feng is chosen, is put in parasitic cage, is supplemented the nutrients with the syrup of 10%-20% for it;
(4) parasitism of 3 instar larvae of diamondback moth is with raising: with 2 250 mL beakers or plastic cup, putting a cabbage leaves in every glass
Piece accesses 200 or so 3 instar larvaes of diamondback moth, is put into the parasitic cage of step (3) for its parasitism, takes out after 1 day, will be parasitic
Larva choose into clean plastic cup, every glass is no more than 50, and the fresh cabbage leaves of supplement, tighten rim of a cup with black cloth and be placed in cup
It is raised in 22 DEG C of constant temperature illumination boxs;
(5) plant the selection and rejuvenation of bee: kind bee selection figure is larger and very active at bee, and periodically from field, acquisition semi-closure is curved
Tail ichneumon wasp carries out rejuvenation to the bee colony of indoor raising.
Preferably, 10 cm of length, wide by 10 of oviposition 15 cm of length of cage, wide 10 cm, parasitism cage described in high 20 cm
Cm, high 10 cm.
Preferably, the cabbage field area is depending on raising scale, with the supply of full feeding diamondback moth whole year.
The method of indoor mass rearing semi-closure initial bow of the invention, by raising and semi-closure initial bow in diamondback moth room
Indoor propagation organically combines, using parasitic cage by parasitic wasp parasitism, to achieve the purpose that obtain mass propagation semi-closure initial bow.
Key of the invention is the parasitism of 3 instar larvae of diamondback moth, guarantees to maintain 10 queen bees in each parasitic cage daily, guarantee daily
There is consistent 3 instar larvae of the diamondback moth supply of the development of sufficient health not select in diamondback moth raising and parasitic processes
Common Turnip Sprouts, Brassica campestris L seedling or cabbage plant are directly raised, and are selected in beaker vessel and are directly raised with cabbage leaves,
Avoid diamondback moth larvae pierce in vermiculite or soil or seedling-raising box outside, be difficult to find, while facilitating operation, it is sub that every cephalont can be grasped
Developmental stage, convenient for the collection of parasitic bee pupa.Ovum is put into plastic casing in step 2 and is cultivated, black cloth is coverd with, and rubber band is pricked
Tightly, it can avoid Eggs of Diamondback Moth around in insect cage or incubator after hatching as larva to climb, pickles raised in insect box
Moth can grasp diamondback moth developmental stage at any time, parasitism is used for conducive to suitable 3 instar larvae is finally selected, in addition, the curved tail Ji of semi-closure
Bee imago needs to supplement the nutrients immediately after sprouting wings, if adult can die in 1-2 days not to supplementing the nutrients, supplements the nutrients, some
The syrup that can even survive 20 days or so at bee, therefore supplement 10%-20% in step (3) is to supplement the nutrients at bee.Using
The parasitic efficiency of method semi-closure initial bow of the invention is very high, general parasitic 6 hours, parasitic rate can reach 80% with
On, the parasitic time is 1 day in parasitic cage in step (4), parasitic overlong time is avoided, there is hyperparasitism, influences survival rate,
Long-term breeding has the population deterioration imagination to semi-closure initial bow indoors, and reproductive capacity and viability can decline, therefore, in field
Diamondback moth will acquire honeybee kind when occurring from field and introduce indoor population, carry out rejuvenation to indoor population to guarantee semi-closure initial bow
Reproductive capacity and viability.
Specific embodiment
With reference to embodiment, technical solution of the present invention is described in more detail;
A kind of method of interior mass rearing semi-closure initial bow, the specific steps of which are as follows:
One, working environment
Wild cabbage plants field: spring and summer can plant wild cabbage in outdoor cabbage field, and autumn and winter wild cabbage is planted in greenhouse, it is ensured that whole year has
Wild cabbage supply;
Diamondback moth Reproduction Conditions: in illumination box or insectary, raising temperature is 25 DEG C or so;
Semi-closure initial bow Reproduction Conditions: in illumination box or insectary, raising temperature is 22 DEG C or so;
Diamondback moth adult mate and oviposit cage: oviposition cage size is long 15 cm, wide 10 cm, high 20 cm wooden frame, bottom plank
Closing, above, two sides are closed with the stainless steel cloth of the mm of 0.5 mm × 0.5, and front is closed with velcro, and hair side is fixed on
On wooden frame, hook surface is fixed on the surrounding of stainless (steel) wire, seals after fitting, and an osculum is torn from below and is operated;
Parasitic cage: size is the wooden frame of long 10 cm, wide 10 cm, high 10 cm, and surrounding diamondback moth is as mate and oviposit cage
It is closed with stainless steel gauze, opening is closed with velcro;
Sucrose water: the sucrose water that configuration concentration is 10% is for being diamondback moth adult and semi-closure initial bow Adult supplement nutrient;
Two, head cabbage varieties: the rich series in capital.
The plantation of wild cabbage
Cabbage seedling is cultivated indoors or in protecting field, is transplanted to crop field or greenhouse later, regular watering, Common Cultivation are needed after transplanting
Period does not spray any pesticide, such as damaged by vermin, can carry out artificial capture method.
Three, the raising of diamondback moth
1. receiving ovum
Ovum is received from oviposition cage daily, there was only ovum on two days received cabbage leaves of head, is put in plastic casing, black cloth is coverd with, rubber
Muscle is put in 25 DEG C of incubators after tightening.
2. larva raising
Ovum is hatched after general 3 days, to be spread a fresh blade in plastic box bottom two days later, checked plastic casing daily, if
Blade is dry or blade is eaten to be not enough to and continues to 1 day appetite of diamondback moth in box, it is necessary to fresh blade is added, it is fresh
Blade will be put in plastic box bottom, it is mouldy, eat surplus, dry blade and throw away, larva above is chosen into fresh blade
On;
Plastic casing is replaced according to the actual situation, and if humidity is excessive in compartmentalized box for holding assorted fruits and candies, faecal contamination is serious, can replace daily, such as compartmentalized box for holding assorted fruits and candies
Interior larva is excessive, can also be changed without, and the worm time is chosen in saving;
Plastic casing is only used for the raising of 1-3 instar larvae, and diamondback moth grew to for 4 ages, it is necessary to and it chooses in plastic cup and raises, Zhi Daohua
Pupa is collected after pupa, is used for follow-on raising;
In parasitic wasp parasitism, required larva is 3 instar larvaes, and the main purpose of larva raising is that acquisition development is neat, standard is consistent
3 instar larvaes, after larva reached for 3 ages, if excessively, the same day with not coming, can first be put in 4 DEG C of refrigerators, it is fast to slow down its development
Degree.
3. the collection of pupa
4 instar larvaes being put in plastic cup can replace primary fresh cabbage leaves for 2 days, and in replacement process, some larvas may
Prepupa pupates, therefore can be collected into plastic culture dish, writes the date with marking pen in ware lid, if the pupa being collected into
It is not eager that it is allowed to sprout wings, can be placed in 4 DEG C of refrigerators and save, date saved is no more than 1 month, otherwise seriously affects its oviposition.
4. the emergence and oviposition of adult
The pickles chrysalis saved in refrigerator is put into insect cage, one big culture dish or disk are put in the inside, are put with small culture dish
One fritter contains the cotton ball of 10% syrup for Adult supplement nutrient, and puts one with a thickness of 2-2.5 mm cabbage leaves by its production
Ovum replaces fresh blade and cotton ball daily, until no longer laying eggs.
Four, semi-closure initial bow indoor propagation technology
Parasitic wasp oviposition completes ovum and larval phase in diamondback moth larvae body in diamondback moth larvae body;
1. the oviposition of parasitic wasp
Parasitic wasp 10 is right, and sex ration is that 1:1 is put in parasitic cage, is supplemented the nutrients with syrup for it, with 2 250 mL beakers or greatly
A cabbage leaves are put in plastic cup, the inside, and 200 or so 3 instar larvaes of diamondback moth of every glass of access are put into parasitic cage and supply it parasitic, and 1
It takes out, is replaced into clean plastic cup after it, every glass is no more than 50 larvas, supplements fresh blade, tightens rim of a cup with black cloth
It raises, the diamondback moth in cage is chosen in cup with tweezers.
2. receiving pupa
The same diamondback moth of method is replaced primary fresh blade in parasitic diamondback moth every two days in beaker or plastic cup, and will be pre-
Pupa is chosen into plastic culture dish, if there is diamondback moth pupates, chooses in the culture dish of pickles chrysalis, and parasitic wasp pupates generally
It pupates than diamondback moth late, therefore diamondback moth prepupa generally just will become parasitic bee pupa after 2 days, needs to choose pupa into parasitic wasp at this time
In the culture dish of pupa.
Bee pupa can be put into 4 DEG C of refrigerators and save if not urgently needed, the holding time preferably no more than 20 days, otherwise eclosion rate meeting
Decline.
3. the emergence of parasitic wasp
The parasitic wasp being stored in refrigerator is put into small insect cage, makes its emergence under the conditions of 22 DEG C, and providing syrup is it
It supplements the nutrients.
4. the selection and rejuvenation of kind of bee: kind bee selection figure is larger and very active at bee, occurs in field diamondback moth
When from field acquisition honeybee kind introduce indoor population, rejuvenation is carried out to indoor population.
As can be seen from the above table, parasitic ratio is parasitic 40 3 instar larvaes of diamondback moth of every queen bee, and parasitic rate is higher, posts
Raw rate reaches 90.52%.
As can be seen from the above table, the parasitic rate of 3 instar larvae of semi-closure initial bow parasitism diamondback moth reaches 80% or more, parasitism
Rate is high.
Table 3 is after semi-closure initial bow is 6 hours parasitic, and parasitic rate can reach 80% or more.
Claims (3)
1. a kind of method of interior mass rearing semi-closure initial bow, it is characterised in that include the next steps:
(1) plantation of diamondback moth host plant wild cabbage: wild cabbage is transplanted to field planting after nursery according to a conventional method, and winter need to be big
The interior plantation of canopy, when wild cabbage grows to 10 leaves or more, picking blade is used for the raising of diamondback moth, during wild cabbage is planted and is harvested not
Spray any medicament;
(2) the indoor raising of diamondback moth: the pickles chrysalis that will sprout wings is placed in oviposition cage, cotton ball is dipped into 10% sucrose
Water is Adult supplement nutrient, and the cabbage leaves for putting a 2-2.5 mm thickness are replaced fresh blade daily and contained by its oviposition
Ovum leaf is placed in 15 cm of diameter, in high 5 cm polyethylene round plastic box, black cloth covers until no longer lay eggs by the cotton ball of syrup
On, rubber band tightens, and plastic casing is put into 25 DEG C of temperature, humidity is 70% or so, is carried out in the incubator of 16: 8 h of photoperiod
Culture spreads a fresh cabbage leaves in plastic box bottom two days later and eats for larva, checks plastic casing daily, add as needed
Add fresh blade, it is mouldy, eat surplus, dry blade and throw away, the larva above blade is chosen into fresh leaf on piece;
(3) in semi-closure initial bow room parasitic environment building: take parasitic wasp, queen bee is 10, is 1 ︰ 1-1.2 according to sex ration
Xiong Feng is chosen, is put in parasitic cage, is supplemented the nutrients with the syrup of 10%-20% for it;
(4) parasitism of 3 instar larvae of diamondback moth is with raising: with 2 250 mL beakers or plastic cup, putting a cabbage leaves in every glass
Piece accesses 200 or so 3 instar larvaes of diamondback moth, is put into the parasitic cage of step (3) for its parasitism, takes out after 1 day, will be parasitic
Larva choose into clean plastic cup, every glass is no more than 50, and the fresh cabbage leaves of supplement, tighten rim of a cup with black cloth and be placed in cup
It is raised in 22 DEG C of constant temperature illumination boxs;
(5) plant the selection and rejuvenation of bee: kind bee selection figure is larger and very active at bee, and periodically from field, acquisition semi-closure is curved
Tail ichneumon wasp carries out rejuvenation to the bee colony of indoor raising.
2. the method for interior mass rearing semi-closure initial bow according to claim 1, it is characterised in that the oviposition cage
15 cm of length, wide 10 cm, parasitism cage described in high 20 cm 10 cm of length, wide 10 cm, high 10 cm.
3. the method for interior mass rearing semi-closure initial bow according to claim 1, it is characterised in that the cabbage field
Area is depending on raising scale.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201811289326.5A CN109197786B (en) | 2018-10-31 | 2018-10-31 | Method for breeding large amount of semi-closed curvicauda curvularia indoors |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201811289326.5A CN109197786B (en) | 2018-10-31 | 2018-10-31 | Method for breeding large amount of semi-closed curvicauda curvularia indoors |
Publications (2)
Publication Number | Publication Date |
---|---|
CN109197786A true CN109197786A (en) | 2019-01-15 |
CN109197786B CN109197786B (en) | 2021-10-29 |
Family
ID=64997667
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201811289326.5A Active CN109197786B (en) | 2018-10-31 | 2018-10-31 | Method for breeding large amount of semi-closed curvicauda curvularia indoors |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN109197786B (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111034684A (en) * | 2019-12-23 | 2020-04-21 | 云南省农业科学院农业环境资源研究所 | Semitrella semipenguii seed conservation and rejuvenation breeding method |
Citations (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB2000007A (en) * | 1977-06-27 | 1979-01-04 | Koppert P | Article and Method for Combatting Harmful Insects in Horticulture |
CN101507425A (en) * | 2009-03-13 | 2009-08-19 | 云南省农业科学院农业环境资源研究所 | Diadegma semiclausum Hellen scale standard breeding technique |
CN101507424A (en) * | 2009-03-13 | 2009-08-19 | 云南省农业科学院农业环境资源研究所 | Cabbage moth scale standard propagation technique |
CN103858827A (en) * | 2014-02-20 | 2014-06-18 | 云南省农业科学院农业环境资源研究所 | Method for carrying out indoor large-scale diadegma semiclausum hellen breeding |
CN204090802U (en) * | 2014-09-01 | 2015-01-14 | 浙江大学 | A kind of extensive raising diamond-back moth adult device |
CN104304183A (en) * | 2014-08-27 | 2015-01-28 | 浙江大学 | Artificial propagation and production method for diadromus collaris |
CN204335591U (en) * | 2014-12-08 | 2015-05-20 | 云南省农业科学院农业环境资源研究所 | A kind of pest natural enemy insect semi-closure initial bow field adaptability device |
CN106106375A (en) * | 2016-06-30 | 2016-11-16 | 云南省农业科学院农业环境资源研究所 | A kind of method of water planting Caulis et Folium Brassicae capitatae indoor mass rearing diamondback moth |
CN106489846A (en) * | 2016-09-20 | 2017-03-15 | 浙江大学 | Diamondback moth artificial propagation production method and its application in biological control |
CN106962293A (en) * | 2017-04-05 | 2017-07-21 | 山西省农业科学院植物保护研究所 | A kind of single head diamondback moth indoor feeding method |
CN107996523A (en) * | 2017-12-06 | 2018-05-08 | 福建农林大学 | The method of indoor rapid amplifying breeding brassicaceous vegetable lepidoptera pest parasitic wasp |
-
2018
- 2018-10-31 CN CN201811289326.5A patent/CN109197786B/en active Active
Patent Citations (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB2000007A (en) * | 1977-06-27 | 1979-01-04 | Koppert P | Article and Method for Combatting Harmful Insects in Horticulture |
CN101507425A (en) * | 2009-03-13 | 2009-08-19 | 云南省农业科学院农业环境资源研究所 | Diadegma semiclausum Hellen scale standard breeding technique |
CN101507424A (en) * | 2009-03-13 | 2009-08-19 | 云南省农业科学院农业环境资源研究所 | Cabbage moth scale standard propagation technique |
CN103858827A (en) * | 2014-02-20 | 2014-06-18 | 云南省农业科学院农业环境资源研究所 | Method for carrying out indoor large-scale diadegma semiclausum hellen breeding |
CN104304183A (en) * | 2014-08-27 | 2015-01-28 | 浙江大学 | Artificial propagation and production method for diadromus collaris |
CN204090802U (en) * | 2014-09-01 | 2015-01-14 | 浙江大学 | A kind of extensive raising diamond-back moth adult device |
CN204335591U (en) * | 2014-12-08 | 2015-05-20 | 云南省农业科学院农业环境资源研究所 | A kind of pest natural enemy insect semi-closure initial bow field adaptability device |
CN106106375A (en) * | 2016-06-30 | 2016-11-16 | 云南省农业科学院农业环境资源研究所 | A kind of method of water planting Caulis et Folium Brassicae capitatae indoor mass rearing diamondback moth |
CN106489846A (en) * | 2016-09-20 | 2017-03-15 | 浙江大学 | Diamondback moth artificial propagation production method and its application in biological control |
CN106962293A (en) * | 2017-04-05 | 2017-07-21 | 山西省农业科学院植物保护研究所 | A kind of single head diamondback moth indoor feeding method |
CN107996523A (en) * | 2017-12-06 | 2018-05-08 | 福建农林大学 | The method of indoor rapid amplifying breeding brassicaceous vegetable lepidoptera pest parasitic wasp |
Non-Patent Citations (4)
Title |
---|
刘树生等: "小菜蛾重要寄生蜂_半闭弯尾姬蜂在中国的地理分布", 《植物保护学报》 * |
杨峰山等: "《小菜蛾Bt毒蛋白受体基因克隆及序列分析》", 31 August 2009, 黑龙江大学出版社 * |
王燕等: "人工繁殖半闭弯尾姬蜂性比失调因素研究", 《公共植保与绿色防控》 * |
陈宗麒等: "小菜蛾弯尾姬蜂室内批量繁殖的技术", 《昆虫天敌》 * |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111034684A (en) * | 2019-12-23 | 2020-04-21 | 云南省农业科学院农业环境资源研究所 | Semitrella semipenguii seed conservation and rejuvenation breeding method |
Also Published As
Publication number | Publication date |
---|---|
CN109197786B (en) | 2021-10-29 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN102125002B (en) | Method for artificially raising plant bug parasitic wasps | |
CN106954592B (en) | Large-scale breeding method for Alliid lice spikemoss | |
CN112205358B (en) | Indoor population propagation method for spodoptera frugiperda | |
CN103155906A (en) | Aphidoletes aphidimyza mass reproduction technology and application technology | |
CN101015290B (en) | Indoor feeding method for wild silkworm | |
CN106900656B (en) | A kind of cigarette fleahopper industrial production method | |
CN106106374B (en) | A kind of biological control method of bright abdomen glaze chalcid fly | |
CN105918200A (en) | Crab-rice symbiotic planting-breeding pool and comprehensive temperature-adjusting method thereof | |
CN113080147A (en) | Method for preventing and treating yellow leaf borers by trichogramma | |
CN103766324A (en) | Method for biologically preventing green plant bugs by peristenus spretus in winter jujube orchard | |
CN113519468B (en) | Method for feeding phyllotreta striolata in indoor generation | |
CN113100002B (en) | Soybean-clanis bilineata tsingtauica one-year three-season planting and breeding mode | |
CN101019522A (en) | Large scale artificial spotted butterfly cultivating technology | |
CN104604809A (en) | Biological control method using encarsia formosa for controlling tobacco whitefly | |
CN109197786A (en) | A kind of method of interior mass rearing semi-closure initial bow | |
CN106472431B (en) | A kind of collection method of Serangium japonicum pupa and the artificial large-scale breeding method of Serangium japonicum | |
CN112471082B (en) | Broadband preferred aphid breeding method | |
CN108094331A (en) | A kind of narrow edge applies the indoor feeding method of noctuid | |
CN107581163B (en) | Method for feeding cryptopterus gracilis by using tetranychus cinnabarinus | |
CN111034684A (en) | Semitrella semipenguii seed conservation and rejuvenation breeding method | |
CN110959581A (en) | Artificial propagation method for psylla chinensis parasitic wasps and application thereof | |
CN111642465A (en) | Breeding method of Altai hepialus larvae | |
CN107279073B (en) | Indoor breeding method for coconut leaf moths | |
CN104322454A (en) | Preservation method of empoasca flavescens living bodies | |
CN101940193B (en) | Method for indoor propagation and outdoor release of cybocephalus |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant |