CN1080994C

CN1080994C - Small egg intermediate host industrial production process of oophagous trichogrammae

Info

Publication number: CN1080994C
Application number: CN99122546A
Authority: CN
Inventors: 王欣; 黄跃革; 贾乃新; 付文玉
Original assignee: CHANGCHUN HONGDA AGRICULTURAL BIOTECHNOLOGY DEVELOPMENT Co Ltd
Current assignee: CHANGCHUN HONGDA AGRICULTURAL BIOTECHNOLOGY DEVELOPMENT Co Ltd
Priority date: 1999-11-19
Filing date: 1999-11-19
Publication date: 2002-03-20
Anticipated expiration: 2019-11-19
Also published as: CN1253723A

Abstract

The present invention discloses a small egg intermediate host industrial production process of oophagous trichogrammae, which takes gelechiid small eggs as a host. The technical processes of three-dimensional high-density cultivation, light-tight vacuum vertical collection of moths, automatic temperature-control closed dust removal, continuous breeding of oophagous trichogrammae of ova solitaria, mechanized quantitative card making, intermediate and short period storage of oophagous trichogrammae, etc. are used for industrially producing oophagous trichogrammae. Because of adopting small eggs as intermediate hosts, the artificial propagation is free from the limitation of areas and time. The present invention has multiple advantages of multiple species of oophagous trichogrammae, broad application, obviously enhanced quality of using intermediate host eggs for producing oophagous trichogrammae, strong adversity resistance and high parasitic rate, and is widely used for biological control of insect pests in agriculture and forestry.

Description

Oophagous trichogrammae technology is produced in the batch production of ovule vector

The present invention relates to the cultural method of oophagous trichogrammae, exactly is to have invented a kind of ovule vector batch production to produce oophagous trichogrammae technology, belongs to the biological control technical field of pest.

At present, mainly be to use large-scale ovum such as tussah to do vector in the production of China oophagous trichogrammae breeding, as the trichogramma dendrolimi that produces with the batch production that realizes.Its shortcoming is that control agricultural pests effect is lower, and the honeybee kind is single, and range of application is narrow, has limited breeding of some good honeybee kinds.

Purpose of the present invention is intended to invent a kind of ovule vector batch production and produces oophagous trichogrammae technology, has solved that big parasitic bee honeybee kind is single, range of application is narrow, has controlled the shortcoming of deinsectization weak effect.

For achieving the above object, the present invention conceives as follows: utilize the gelechiid ovule as the host, three-dimensional High Density Cultivation adopts lucifuge, the vertical receipts of vacuum moth, temperature automatically controlled sealing dedusting, and the continuous numerous honeybee of laying off eggs, honeybee is store in the quantitative fabrication of mechanization a middle or short term.Concrete production technology is:

1, kill the ovum sterilization: with the wheat impurity that sieves out, add the sterilization of heat kill ovum, temperature is 60～95 ℃, and the time is 15～120 minutes, and wheat is played emollescence;

2, connect the ovum parasitism: making the wheat water content is 14～18%, and wheat, moth ovum ratio are 1000: 0.7～1.0, and gelechiid ova is evenly dispersed on the wheat, and temperature remains on 24 ± 2 ℃ and 85 ± 5RH and makes it moth and go into;

3, larva is cultivated: the wheat culture plate of inoculation is placed in the greenhouse cultivated, the holding chamber inner air flow, each coils the temperature unanimity, it is neat that gelechiid is grown, consider that larvae development produces metabolic heat, the temperature control method of Gao Houzhong before adopting, after parasitic 7～10 days, wheat product temperature raises gradually, room temperature remains on below 20 ℃, RH75～80%, stirring can be lowered the temperature below 30 ℃ to wheat, and the product temperature reduces gradually after 12 days, larva begins to pupate, for preventing the wheat bacterium of mildewing, the potassium permanganate solution with 0.1% drenches wheat, and culture period is limited to 22～30 days.

4, adult is collected: adopt the vertical lucifuge vacuum of sealing to take out the moth technology, when adult begins to sprout wings, wheat is packed in the flat cage of eye with holes, and tiltedly be placed in the adult collecting box, constantly ventilating makes adult be collected in the gatherer by air flow, and the time is 18～20 days;

5, receive the preservation of ovum, clean ovum and ovum: the adult of collecting is put into the cage of laying eggs, under the condition of dark and extra-nutrition, laid eggs 3～5 days, the ovum after collecting through removing out impurity, is put under 3 ± 1 ℃ of conditions and preserved;

6, the continuous numerous honeybee of mechanization: adopt the dark (L: D) be 1: 12 continuous numerous honeybee method of cellular-type of light;

7, the quantitative fabrication of mechanization: quantitatively spread ovum, make the quantity of oophagous trichogrammae on the unit are ovum card identical, be 3000 of single deck tape-recorders;

8, connect honeybee, storage honeybee: connecing the honeybee time is 24～72 hours, and the storage honeybee time is 30～180 days.

Good effect of the present invention is: adopt ovule as vector, artificial propagation is not subjected to area and time restriction, and numerous honeybee kind is many, applied range, and numerous honeybee quality performance of vector ovum is obviously, honeybee kind strong stress resistance, improve parasitic rate, and demonstrate multiple advantage.

Further specify the present invention below in conjunction with embodiment:

Embodiment 1

Get wheat 1000g and remove out impurity, put into electric heating rotation sterilization stove and kill the ovum sterilization, temperature is controlled at 70～85 ℃, and the time is 90 minutes, make moisture about 16% connect the ovum wheat; Get 3～5 days gelechiid ova of fresh firm product, took out in preceding 58 hours in inoculation, sterilized 10 minutes with 10% formalin earlier, used flushing with clean water again 5 minutes, put into then under the environmental condition of 25 ± 1 ℃ and RH85 ± 5% and grow, the aqueous solution of ovum or ovum divided equably to be sprinkling upon on the wheat before ovum hatching, the per kilogram wheat restrains ovum with 0.9, the culturing room that puts into 24 ± 2 ℃ and 85 ± 5RH after the inoculation make its eat into into; Culture plate is placed on the iron shelf, ventilates, guarantee the temperature unanimity of each culture plate, after parasitic 7 days, larvae development produces metabolic heat, and wheat product temperature raises gradually, this moment, room temperature remained on below 20 ℃, RH75～5%, stirring can be lowered the temperature to wheat, for preventing the wheat bacterium of mildewing, potassium permanganate solution with 0.1% is given the wheat humidification, later product temperature reduced gradually in 12 days, and larva begins to pupate, and was inoculated into gelechiid and sprouted wings greatly about 28 days.When adult begins to sprout wings, to pack into wheat in the flat cage of eye with holes and tiltedly stand up in the adult collecting box, ventilation unit can disperse the metabolic heat that larvae development produces, and adult is collected in the adult gatherer by air flow, and adult is collected and finished in 20 days.The adult of collecting is quantitatively put in the cage of laying eggs, and be fixed on the oopod of rotation, under the condition of dark and extra-nutrition, laid eggs 4 days.The ovum of collecting is put into removes foreign material such as a mao leg, moth wing on the reverse clean egg apparatus, with the further cleaning of 20～90 purpose series standards sieve, be placed in 3 ± 1 ℃ of refrigerators and preserve then, the numerous moth ovum of storage life is no more than 5 days, and numerous honeybee is no more than 10 days with ovum.Adopt the continuous numerous bee technology of cellular-type, Light To Dark Ratio is 1: 12.Quantitatively spread ovum by mechanical clip making machine, go out 2500 of honeybees on the single deck tape-recorder.

Claims

1, oophagous trichogrammae technology is produced in a kind of ovule vector batch production, comprises following technical process:

(1), kill the ovum sterilization: with the wheat impurity that sieves out, add the sterilization of heat kill ovum, temperature is 60～95 ℃, and the time is 15～120 minutes, and wheat is played emollescence;

(2), connect the ovum parasitism: making the wheat water content is 14～18%, and wheat, moth ovum ratio are 1000: 0.7～1.0, and gelechiid ova is evenly dispersed on the wheat, and temperature remains on 24 ± 2 ℃ and 85 ± 5RH and makes it moth and go into;

(3), larva is cultivated: the wheat culture plate of inoculation is placed in the greenhouse cultivated, the holding chamber inner air flow, each coils the temperature unanimity, it is neat that gelechiid is grown, consider that larvae development produces metabolic heat, the temperature control method of Gao Houzhong before adopting, after parasitic 7～10 days, wheat product temperature raises gradually, room temperature remains on below 20 ℃, RH75～80%, stirring can be lowered the temperature below 30 ℃ to wheat, and the product temperature reduces gradually after 12 days, larva begins to pupate, for preventing the wheat bacterium of mildewing, the potassium permanganate solution with 0.1% drenches wheat, and culture period is limited to 22～30 days.

(4), adult is collected: adopt the vertical lucifuge vacuum of sealing to take out the moth technology, when adult begins to sprout wings, wheat is packed in the flat cage of eye with holes, and tiltedly be placed in the adult collecting box, constantly ventilating makes adult be collected in the gatherer by air flow, and the time is 18～20 days;

(5), the preservation of receiving ovum, clean ovum and ovum: the adult of collecting is put into the cage of laying eggs, under the condition of dark and extra-nutrition, laid eggs 3～5 days, the ovum after collecting through removing out impurity, is put under 3 ± 1 ℃ of conditions and preserved;

(6), the continuous numerous honeybee of mechanization: adopt the dark (L: D) be 1: 12 continuous numerous honeybee method of cellular-type of light;

(7), the quantitative fabrication of mechanization: quantitatively spread ovum, make the quantity of oophagous trichogrammae on the unit are ovum card identical, be 3000 of single deck tape-recorders;

(8), connect honeybee, storage honeybee: connecing the honeybee time is 24～72 hours, and the storage honeybee time is 30～180 days.