CN107439491B - Reinforce the pupa parasitic wasp artificial breeding production method of drosophila class biological control effect - Google Patents
Reinforce the pupa parasitic wasp artificial breeding production method of drosophila class biological control effect Download PDFInfo
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
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- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
- A23K20/147—Polymeric derivatives, e.g. peptides or proteins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/24—Compounds of alkaline earth metals, e.g. magnesium
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/90—Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
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Abstract
The present invention relates to agricultural pests field of biological control, it is desirable to provide a kind of pupa parasitic wasp artificial breeding production method for reinforcing drosophila class biological control effect.This method comprises: collecting the Drosophila melanogaster pupa of coat angle clavicorn serphid parasitism, continuous three generations is used for the parasitism of sea De Shi drosophila pupa after the bee out that sprouts wings, and collects honeybee kind；It to extra large De Shi drosophila inoculation reproduction, is then pupated with the queen bee of parasitic wasp to extra large De Shi drosophila and carries out parasitic, culture, collected parasitic wasp and preservation after sprouting wings and reserve seed for planting.The present invention controls a mao parasitic time for angle clavicorn serphid in production seeded process, avoids the low bee hatching rate caused by low parasitic rate caused by the parasitic time is too short or due to excessive parasitism；The reasonable inoculation and utilization in parasitic wasp more convenient to the cultivation of hair angle clavicorn serphid；It is able to carry out large-scale continuous production, improves advantage Tropical area and numerous bee technology, realizes high-quality, efficient, high-volume breeding hair angle clavicorn serphid, is the breeding of hair angle clavicorn serphid using having established good basis.
Description
Technical field
The present invention relates to the pupa of agricultural pests field of biological control, in particular to reinforcement drosophila class biological control effect is parasitic
Bee artificial breeding production method belongs to the rearing method of advantage host and to reinforcement drosophila class pest biological control effect
Pupa parasitic wasp artificial breeding production method.
Background technique
Drosophila feeding habits are extensive, and growing environment is special, like dark moist habitat, have to sour-sweet and corrupt smell bright
Aobvious taxis, the succulence organ of can cause harm a variety of gourd, fruit and vegetables and many plants are important agricultural pests.When drosophila endangers farming
Exist usually in the form of hybrid when object, including Drosophila melanogaster Drosophila melanogaster, her rice drosophila
D.immigrans, spotted wing drosophila D.suzukii etc..Parasitic wasp can control the population quantity of host, be important biological control
The factor.Therefore, Population Control is carried out to drosophila class pest using natural enemy insect more and more in integrated pest control management.
Hair angle clavicorn serphid Trichopria drosophilae Perkins, belongs to Hymenoptera Diapriidae, is drosophila
The main parasitic bee in pupa time, host range is wide.In laboratory conditions, parasitic efficiency is higher than ring abdomen cynipidae Figitida's
Two kinds of main drosophila larvae parasitic wasps, but it is seldom directed to drosophila pupa time parasitic wasp in the research of drosophila-parasitic wasp system at present,
And hair angle clavicorn serphid is even more to be rarely reported before spotted wing drosophila becomes global pest.
The Chinese patent of application number CN201610213523.3 discloses a kind of method of indoor breeding hair angle clavicorn serphid,
But the development of host's spotted wing drosophila group worm state as employed in embodiment is different, and artificial breeding complicated condition, is unfavorable for
Standardization and extensive raising, therefore do not offer convenience to largely obtaining hair angle clavicorn serphid in actual production；Application number
A kind of method for cultivating fly pupa tiny golden wasp using Hosts of the Introduction To Cn Patent of CN201210448362.8, the embodiment
In derived from the parasitic wasp of advantage host there is stronger control effect, but the survival ability of fly pupa tiny golden wasp and parasitic effect to drosophila
The items biological characteristics such as rate are not as good as hair angle clavicorn serphid.
The hair angle clavicorn serphid eclosion rate and parasitism of development from Drosophila melanogaster pupa are high-efficient, and the service life is long, and fertility is strong, is
Prevent and treat the good source in terms of drosophila.But compared to Drosophila melanogaster, extra large De Shi drosophila D.hydei longer life expectancy, reproductive capacity is stronger,
And each stage of development individual is bigger, is the advantage Hosts for breeding parasitic wasp.There has been no biological control hair angle hammers for the existing country
Angle serphid simultaneously applies it to the research report in terms of preventing and treating drosophila class pest, if energy vast propagation hair angle clavicorn serphid, right
The effect for improving control of insect in actual production has important reference significance.
Summary of the invention
The technical problem to be solved by the present invention is to overcome deficiency in the prior art, provide a kind of reinforcement drosophila class biology
The pupa parasitic wasp artificial breeding production method of control efficiency.
In order to solve the above technical problems, solution of the invention is:
A kind of pupa parasitic wasp artificial breeding production method for reinforcing drosophila class biological control effect, including following steps are provided
It is rapid:
(1) collect and expand numerous honeybee kind
It is permanent to be placed in the constant temperature that temperature is 25 ± 1 DEG C, RH is 50% for the Drosophila melanogaster pupa for collecting coat angle clavicorn serphid parasitism
It is developed in wet incubator；Pupa wait be collected into is sprouted wings after bee out, first it is made sufficiently to mate, and is then 1: 10 by queen bee and fly pupa
Ratio be placed in the culture bottle equipped with hair angle clavicorn serphid food carry out it is parasitic expand numerous, finished to the oviposition of queen bee parasitism and move queen bee
Out；
By obtained hair angle clavicorn serphid offspring, continuous three generations is used for the parasitism of extra large De Shi drosophila pupa in the same way, makes
Hair angle clavicorn serphid population from extra large De Shi drosophila pupa is stabilized, and finally collects 50 or so bodies and grows at 4.5~4.9 millimeters
Drone and 200 or so bodies grow the honeybee kind in 3.6~4.0 millimeters of queen bee as artificial propagation, raising is 18 in temperature
± 1 DEG C, the interior preservation of the constant temperature and humidity incubator that RH is 50%；
(2) inoculation reproduction of sea De Shi drosophila
The ratio of extra large De Shi drosophila 25 for taking or so emergence latter week and sufficiently mating, female drosophila and male drosophila is 20:
5；With CO2It anaesthetizes and is put into the culture bottle equipped with sea De Shi drosophila food, cultivated in 25 DEG C of insulating box to oviposition 100-
Drosophila is taken out after 120；Culture bottle is placed in the constant temperature and humidity incubator that temperature is 25 ± 1 DEG C, RH is 50%, observes and remember
Record starts the time of pupating, number of pupating and Growth of Drosophila Melanogaster situation；
(3) clavicorn serphid in hair angle is inoculated with
After sea De Shi drosophila pupates 2 days in step (2), chosen in the honeybee kind of the hair angle clavicorn serphid saved from step (1)
The queen bee of 1/10 pupa number is put into the former cultivation bottle；Observation queen bee, which pupates to extra large De Shi drosophila, carries out parasitism, takes out and posts after 12h
Raw bee；
(4) culture of hair angle clavicorn serphid and collection
It is trained the constant temperature and humidity that temperature is 25 ± 1 DEG C, RH is 50% is placed in by parasitic extra large De Shi drosophila pupa in step (3)
It supports in case, when parasitic wasp is developed to the 15th day in Host Pupae, parasitic wasp starts to sprout wings successively, collects the parasitism after sprouting wings
Bee；
(5) it reserves seed for planting
The hair angle clavicorn serphid being collected into step (4) is put into the culture tube equipped with parasitic wasp food, culture tube is set
In preservation in the constant temperature and humidity incubator that temperature is 18 ± 1 DEG C, RH is 50%；100 or so are saved altogether, when for next breeding
Hair angle clavicorn serphid inoculation.
In the present invention, the configuration method of the parasitic wasp food are as follows: take 27g agar to be added in 1000ml water, be sufficiently stirred
High-temp steam sterilizing is carried out after mixing；Then 33g brown sugar and the pure cider of 330ml is added, natural cooling after mixing is sufficiently stirred
To 60 DEG C；100g methylparaben is dissolved in 95% ethyl alcohol of 900ml, is then added in the mixture cooled down, sufficiently stirs
Mixing is mixed, 4 DEG C of refrigerations are spare.
In the present invention, the configuration method of the sea De Shi drosophila food are as follows: take dry ferment 80g to be added molten in 300ml warm water
Solution；It weighs agar 10g, tryptone 20g, yeast extract 20g, sucrose 30g, glucose 60g to be added in 700ml warm water, fill
Divide after stirring and evenly mixing and the 300ml yeast soln dissolved is added, heats and stir；Object to be mixed sequentially adds 0.5g when cooked
MgSO4·7H2O and CaCl2·2H2When naturally cooling to 50~60 DEG C after boiling, 6ml propionic acid, 10ml methylparaben is added in O
Solution and 1g sodium benzoate simultaneously stir evenly, and seal after cooling 6~7h, it is spare to be put into 4 DEG C of refrigerator cold-storages；The methylparaben
Solution is to be dissolved in 95% ethyl alcohol of 900ml with 100g methylparaben to form.
In the present invention, the Drosophila melanogaster pupa for collecting coat angle clavicorn serphid parasitism operates: choosing in the steps below
It selects that insect is active and the season of rare extreme weather, is placed around drosophila parasitic wasp trap at vegetation water source abundant, three
It is recycled after week, collects the Drosophila melanogaster pupa of coat angle clavicorn serphid parasitism in trap.
Inventive principle description:
Hair angle clavicorn serphid parasitic scope is wide, not the obligatory parasitism bee of Drosophila melanogaster, while outdoor trapping honeybee kind,
It is also possible to capture other kinds of Drosophila simultaneously.Wherein individual is big for selection, wild-type Drosophila of high sensitivity, through morphology and
Molecular biology identification is sea De Shi drosophila, as host.If the conventional food formula with laboratory rearing Drosophila melanogaster is (beautiful
Rice flour-yeast-agar) raising sea De Shi drosophila, larva energy is low, and ability of creeping is weak, is often embedded in food bottom and pupates, this is not
Only it is unfavorable for the Reproductive development of host, and makes troubles for the parasitism of hair angle clavicorn serphid and emergence.
The present invention is improved by constantly test, is had adjusted the main nutrient composition ratio of former food preparation, that is, is increased egg
White matter and the intake of sugar shorten its life cycle to not only extend the service life of host drosophila, while spawning rate and pupating
Rate also significantly improves, this provides basic technical support for the artificial biological control effect for reinforcing hair angle clavicorn serphid.
Compared with the hair angle clavicorn serphid for using Drosophila melanogaster fly pupa to breed, the hair angle of extra large De Shi drosophila fly pupa acquisition is used
The morphological feature of clavicorn serphid and in the control to Drosophila melanogaster, efficiency is more prominent, two kinds of propagation method comparing results
As follows: compared with the hair angle clavicorn serphid of experimental determination bred by Drosophila melanogaster fly pupa, the hair angle that the present invention obtains is hammered into shape
Angle serphid has higher eclosion rate and produces female rate, and significantly longer, individual morphology is significantly larger, queen bee is fertile with individual life span
Time significantly extends, stores up smart the features such as ability significantly increases, anti-adversity ability significantly improves；In addition, with Drosophila melanogaster fly pupa is used
It compares, in parasitic Drosophila melanogaster fly pupa, parasitic rate and parasitic efficiency significantly increase the hair angle clavicorn serphid obtained with the present invention
Greatly.
Compared with prior art, the beneficial effects of the present invention are:
1, the present invention reasonable inoculation and utilization in parasitic wasp more convenient to the cultivation of hair angle clavicorn serphid；
2, the present invention controls a mao parasitic time for angle clavicorn serphid in production seeded process, avoids because of the parasitic time
It is too short caused by low parasitic rate or because it is excessive parasitism due to caused by low bee hatching rate；
3, the present invention requires the inoculative proportion of hair angle clavicorn serphid, host's drosophila pupa, by host when inoculation is parasitic
It concentrates in culture bottle, a mao time for angle clavicorn serphid host-location is greatly shortened, to improve the parasitic rate of parasitic wasp；
4, the present invention is able to carry out large-scale continuous production, improves advantage Tropical area and numerous bee technology, realizes high-quality, high
Effect, high-volume breeding hair angle clavicorn serphid, for hair angle clavicorn serphid breeding using having established good basis；
5, the present invention has reference role to the breeding of other bees, storage and release；
6, the present invention improves the control efficiency to the berries class pest such as drosophila, and being applied to Agricultural pest control field can be more
Meet agricultural production needs in big degree.
Detailed description of the invention
Fig. 1 is hair angle clavicorn serphid artificial progagation flow diagram of the invention.
Specific embodiment
Present invention is further described in detail with specific embodiment with reference to the accompanying drawing:
As shown in Figure 1, the inoculation expanding propagation method of hair angle clavicorn serphid includes the following steps:
(1) collect and expand numerous honeybee kind；
Step (1) specifically includes:
A, it collects honeybee kind: selecting insect actively and the season of rare extreme weather, be placed around at vegetation water source abundant
Drosophila parasitic wasp trap (Chinese patent that application reference number is CN201621171038.6), recycles after three weeks, collects quilt
The Drosophila melanogaster pupa of hair angle clavicorn serphid parasitism, is placed in the constant temperature and humidity incubator that temperature is 25 ± 1 DEG C, RH is 50% and (is purchased from
Hangzhou Lai Bo Biotechnology Co., Ltd) in development.
The judgment method of the Drosophila melanogaster pupa of coat angle clavicorn serphid parasitism: the pupa time of normal Drosophila melanogaster is 4 days, so
Aftershaft dissolves drosophila；Drosophila melanogaster pupa after coat angle clavicorn serphid is parasitic needs 15-20 talent to have bee from wherein sprouting wings, and
During young honeybee is developed in drosophila pupa, it can be observed have polypide gradually color burn blackening in translucent puparium.To hair
Angle clavicorn serphid is collected after sprouting wings out of Drosophila melanogaster pupa, is carried out by approach such as taxology and molecular biology to it
Identification confirms that the parasitic wasp is hair angle clavicorn serphid.The present invention hammers hair angle into shape in the numerous equal operation of the expansion after the collection step
The collection of angle serphid directly expands first generation parasitic wasp derived above numerous without field acquisition mode.
B, it parasitic wasp food formulation (1L): weighs 27g agar and is added in 1000ml water, carry out high temperature after mixing is sufficiently stirred
Steam sterilizing；33g brown sugar is then added, mixing is sufficiently stirred in the pure cider of 330ml；It, will after food naturally cools to 60 DEG C
100g methylparaben is dissolved in 95% ethyl alcohol of 900ml, is sufficiently dissolved and is added in the food cooled down, is sufficiently stirred mixed
It is even；Food is dispensed to saturating benzene drosophila pipe and plastics raising bottle (purchase is in Zhong Tai experiment equipment factory, Haimen City), 4 DEG C of refrigerations are spare.
C, honeybee kind expands numerous: the pupa after be collected into step A is sprouted wings after bee out, first it is made sufficiently to mate, and presses queen bee and fly afterwards
(drone is not put) in the culture bottle that pupa number places it in step B for the ratio of 1:10, and it is numerous to carry out parasitic expansion.It is produced to queen bee parasitism
Ovum, which finishes, to be moved out.
D, honeybee kind is preferably stablized with population: by the hair angle clavicorn serphid offspring obtained obtained in step C with same side
The continuous three generations of method is used for the parasitism of sea De Shi drosophila pupa, keeps the hair angle clavicorn serphid population from extra large De Shi drosophila pupa steady
It is fixed；Final choice body, which is grown, grows the female peak at 3.6~4.0 millimeters as artificial propagation in 4.5~4.9 millimeters of drone and body
Honeybee kind collects 50 or so hair angle clavicorn serphid Xiong Feng and 200 or so the hair angle female peaks of clavicorn serphid.
E, honeybee kind saves: the hair angle clavicorn serphid that will sprout wings in step D raises the Yu Wen in the parasitic wasp food of step B
In the laboratory constant temperature and humidity incubator (being purchased from hundred Si He Biotechnology Co., Ltd of Nanjing) that degree is 18 ± 1 DEG C, RH is 50%
It saves；
(2) inoculation reproduction of sea De Shi drosophila；
Step (2) specifically includes:
F, sea De Shi drosophila food formulation (1L): weighing dry ferment 80g, with the dissolution of 300ml warm water；Weigh agar 10g, pancreas
Peptone 20g, yeast extract 20g, sucrose 30g, glucose 60g is in 700ml warm water, after mixing is sufficiently stirred, is added
The 300ml yeast soln of dissolution is heated and is stirred energetically；0.5g is separately added into when above-mentioned 1000ml food is substantially cooked
MgSO4·7H2O and CaCl2·2H2O changes small fire, then boils 1-2min, Guan Huo after boiling, naturally cool to 50~60 DEG C to food
When, 6ml propionic acid, 10ml Metagin ester solution (95% ethyl alcohol that 100g methylparaben is dissolved in 900ml) and 1g benzene first is added
Sour sodium dispenses food in the culture vessel in step C, is sealed later with gauze after mixing evenly；To the cooling 6~7h of food
Afterwards, it is sealed, is stuck on a piece of adhesive tape with packaging bag, be put into 4 DEG C of refrigerator cold-storages, it is used as needed.
G, host drosophila inoculation reproduction: take sprout wings it is latter week left and right and sufficiently mate extra large De Shi drosophila 25 (female drosophila:
Male drosophila=20:5), with CO2The culture bottle dispensed in step F is anaesthetized and be put into, is taken after 2h of laying eggs in 25 DEG C of insulating box
Out, make its 100-120 head of laying eggs.
H, the development of fly pupa: the drosophila bottle in 10 bottles of step G is placed in the Intelligent culture case in step A, observes and remembers
Record starts the time of pupating, number of pupating and Growth of Drosophila Melanogaster situation；
(3) clavicorn serphid in hair angle is inoculated with；
Step (3) specifically includes:
After the extra large De Shi drosophila in step H pupates 2 days, the queen bee of 1/10 pupa number is chosen in the honeybee kind that step E is saved, is set
It is carried out in Intelligent culture case in step A parasitic (not putting drone), takes out parasitic wasp after 12h.
When carrying out parasitic inoculation, it is ensured that hair angle clavicorn serphid has been subjected to abundant mating, and energy is strong；Hair angle clavicorn is thin
Bee was in for two age in days pupa time.
(4) culture of hair angle clavicorn serphid and collection；
Step (4) specifically includes:
It will be placed in the Intelligent culture case in step (1) A in step (3) by parasitic drosophila pupa, to parasitic wasp in host
When being developed to the 15th day in pupa, parasitic wasp starts to sprout wings successively；The parasitic wasp of emergence is collected into the food training in step (1) B
It supports in pipe, and in the climatic chamber being stored in step (1) E.
(5) it reserves seed for planting；
Step (5) specifically includes:
It takes hair angle clavicorn serphid about 100 conditions according to step (1) E in step (4) to save honeybee kind, is educated for next time
Hair angle clavicorn serphid inoculation when kind.
Technical effect summary:
Compared with the hair angle clavicorn serphid for using Drosophila melanogaster fly pupa to breed, using the hair of technical solution of the present invention acquisition
Angle clavicorn serphid is respectively 100% (N=10), 96.50% (N to the parasitic rate of Drosophila melanogaster fly pupa, bee hatching rate and the female rate of production
=10) and 84.97% (N=10), with the hair angle clavicorn serphid of Drosophila melanogaster fly pupa breeding to the parasitic rate of Drosophila melanogaster fly pupa,
Bee hatching rate and the female rate of production are respectively 99.50% (N=10), 85.93% (N=10) and 59.65% (N=10).The two is being posted
There was no significant difference in raw rate, but in bee hatching rate (P < 0.05) and produces on female rate (P < 0.05), the hair angle clavicorn that the present invention obtains
Serphid has significant advantage.
The hair angle clavicorn serphid individual that the present invention obtains is bigger, no matter male and female, feeler is long (male: P < 0.01；It is female: P <
0.01), body is long (male: P < 0.01；It is female: P < 0.01), wing it is long (male: P < 0.01；It is female: P < 0.01) development is noticeably greater than from black abdomen
The hair clavicorn serphid individual of drosophila fly pupa.
The hair angle clavicorn serphid fecundity that the present invention obtains is stronger: the maximum fish brood amount of single head queen bee is 133.5 ± 8.0
A (N=20), its single head parasitism of ordering the case where only mating one time, the egg-laying season is 23.3 ± 3.1 days (N=20), average
Service life is 26.2 ± 3.3 days (N=20)；With Drosophila melanogaster fly pupa breeding hair angle clavicorn serphid maximum fish brood amount be 105.0 ±
9.1 (N=20), the egg-laying season is 10.3 ± 0.5 days (N=20), and average life span is 12.2 ± 0.8 days (N=20).Thus may be used
, the hair angle clavicorn serphid service life that the present invention obtains significantly extends (P < 0.01), and the egg-laying season also significantly extends (P < 0.01).This hair
The single head queen bee of the hair angle clavicorn serphid of bright acquisition can be 198.0 ± 18.5 (N for sum in postpartum after only mating is primary
=20), wherein queen bee is 74.0 ± 3.3 (N=20)；With the single head queen bee of the hair angle clavicorn serphid of Drosophila melanogaster fly pupa breeding
It can postpartum be in the same circumstances 119.0 ± 18.7 (N=20) for sum, wherein queen bee is 30.0 ± 7.8 (N=20).
Thus, to store up smart ability stronger for the hair angle clavicorn serphid queen bee that obtains of the present invention, offspring individuals number (P < 0.05) and queen bee (P <
0.05) number shared by is significant more.
The hair angle clavicorn serphid that the present invention obtains also has significant advantage in terms of anti-adversity ability: in 4 DEG C of low temperature and 37 DEG C
Can survive at least 25 days (N=100) and 16 days (N=100) respectively under high temperature, in the case where starvation, can at most survive about 15
Its (N=100)；And it can be survived respectively under 4 DEG C of low temperature and 37 DEG C of high temperature with the hair angle clavicorn serphid that Drosophila melanogaster fly pupa is bred
It at least 15 days (N=100) and 8 days (N=100), in the case where starvation, can at most survive about 7 days (N=100).Thus may be used
, the hair angle clavicorn serphid that the present invention obtains is in adverse circumstances such as low temperature (P < 0.05), high temperature (P < 0.05) and starvation (P < 0.05)
Under survival rate be all remarkably higher than with Drosophila melanogaster fly pupa breed hair angle clavicorn serphid.
The hair angle clavicorn serphid from Drosophila melanogaster fly pupa is developed under 25 DEG C, the laboratory condition that RH is 50%, if without posting
Raw behavior is only capable of survival 40 days or so, and under the same conditions by the hair angle clavicorn serphid of the invention obtained, the 40th after sprouting wings
It parasitic rate, bee hatching rate and the female rate of production still may be up to 97.78 ± 3.14% (N=90), 96.50 ± 2.91% (N=90)
With 72.85 ± 4.58% (N=90).
In conclusion the hair angle clavicorn serphid that the present invention is bred has stronger control efficiency to Drosophila melanogaster.
Finally it should be noted that listed above is only to illustrate specific embodiments of the present invention.Obviously, the present invention is not
It is limited to above embodiments, there can also be many variations.Those skilled in the art can be straight from present disclosure
All deformations for connecing export or associating, are considered as protection scope of the present invention.
Claims (4)
1. a kind of pupa parasitic wasp artificial breeding production method for reinforcing drosophila class biological control effect, which is characterized in that including under
State step:
(1) collect and expand numerous honeybee kind
The Drosophila melanogaster pupa for collecting coat angle clavicorn serphid parasitism is placed in the constant temperature and humidity training that temperature is 25 ± 1 DEG C, RH is 50%
It supports and is developed in case；Pupa wait be collected into is sprouted wings after bee out, first it is made sufficiently to mate, the ratio for being then 1: 10 in queen bee and fly pupa
Be placed in the culture bottle equipped with hair angle clavicorn serphid food carry out it is parasitic expand numerous, finished to the oviposition of queen bee parasitism and remove queen bee；
By obtained hair angle clavicorn serphid offspring, continuous three generations is used for the parasitism of extra large De Shi drosophila pupa in the same way, makes to come from
The hair angle clavicorn serphid population of extra large De Shi drosophila pupa is stabilized；Finally collect 50 bodies grow 4.5 ~ 4.9 millimeters drone and
200 bodies grow the honeybee kind in 3.6 ~ 4.0 millimeters of queen bee as artificial propagation, and raising is in temperature is 18 ± 1 DEG C, RH is
It is saved in 50% constant temperature and humidity incubator；
(2) inoculation reproduction of sea De Shi drosophila
The ratio of the extra large De Shi drosophilas for taking 25 emergence latter week and sufficiently mating, female drosophila and male drosophila is 20: 5；With CO2Fiber crops
It is liquor-saturated and be put into the culture bottle equipped with sea De Shi drosophila food, it is cultivated in 25 DEG C of insulating box to laying eggs and is taken after 100-120
Drosophila out；Culture bottle is placed in the constant temperature and humidity incubator that temperature is 25 ± 1 DEG C, RH is 50%, observes and records beginning
Pupa time, number of pupating and Growth of Drosophila Melanogaster situation；
(3) clavicorn serphid in hair angle is inoculated with
After sea De Shi drosophila pupates 2 days in step (2), 1/10 is chosen in the honeybee kind of the hair angle clavicorn serphid saved from step (1)
The queen bee of pupa number is put into the former cultivation bottle；Observation queen bee, which pupates to extra large De Shi drosophila, carries out parasitism, takes out after 12h parasitic
Bee；
(4) culture of hair angle clavicorn serphid and collection
The constant temperature and humidity incubator that temperature is 25 ± 1 DEG C, RH is 50% will be placed in by parasitic extra large De Shi drosophila pupa in step (3)
Interior, when parasitic wasp is developed to the 15th day in Host Pupae, parasitic wasp starts to sprout wings successively, and the hair angle clavicorn collected after sprouting wings is thin
Bee；
(5) it reserves seed for planting
The hair angle clavicorn serphid being collected into step (4) is put into the culture tube equipped with parasitic wasp food, culture tube is placed in temperature
It is saved in the constant temperature and humidity incubator that degree is 18 ± 1 DEG C, RH is 50%；100 are saved altogether, hair angle when for next breeding
The inoculation of clavicorn serphid.
2. the method according to claim 1, wherein the configuration method of the parasitic wasp food are as follows: take 27g agar
It is added in 1000ml water, carries out high-temp steam sterilizing after mixing is sufficiently stirred；Then 33g brown sugar and the pure cider of 330ml is added,
60 DEG C are naturally cooled to after mixing is sufficiently stirred；100g methylparaben is dissolved in 95% ethyl alcohol of 900ml, is then added cold
In good mixture, mixing is sufficiently stirred, 4 DEG C of refrigerations are spare.
3. the method according to claim 1, wherein the configuration method of the sea De Shi drosophila food are as follows: take dry
Yeast 80g, which is added in 300ml warm water, to be dissolved；Weigh agar 10g, tryptone 20g, yeast extract 20g, sucrose 30g, grape
Sugared 60g is added in 700ml warm water, and the 300ml yeast soln dissolved is added after mixing is sufficiently stirred, heats and stirs；To mixed
It closes when object is cooked and sequentially adds 0.5g MgSO4•7H2O and CaCl2•2H2O when naturally cooling to 50 ~ 60 DEG C after boiling, is added
6ml propionic acid, 10ml Metagin ester solution and 1g sodium benzoate simultaneously stir evenly, and seal after cooling 6 ~ 7h, it is cold to be put into 4 DEG C of refrigerators
It hides spare；The Metagin ester solution is to be dissolved in 95% ethyl alcohol of 900ml with 100g methylparaben to form.
4. the method according to claim 1, wherein the Drosophila melanogaster for collecting coat angle clavicorn serphid parasitism
Pupa operates in the steps below: selecting insect active and the season of rare extreme weather, puts near vegetation water source abundant
Drosophila parasitic wasp trap is set, is recycled after three weeks, the Drosophila melanogaster pupa of coat angle clavicorn serphid parasitism in trap is collected.
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| CN109769755B (en) * | 2019-03-29 | 2021-09-07 | 福建农林大学 | Simple and rapid propagation method of euproctis maculata pupa parasitic wasps, namely horned and branchlet wasps |
| CN110115251A (en) * | 2019-05-27 | 2019-08-13 | 浙江大学 | A method of extending drosophila class pest parasitic wasp shelf life |
| CN110692601A (en) * | 2019-11-12 | 2020-01-17 | 安徽师范大学 | Method for breeding aphelesia crenulata by using drosophila melanogaster to replace drosophila suzukii |
| CN111149772B (en) * | 2020-01-14 | 2020-11-24 | 南京农业大学 | Method for extracting parasitic bee venom by using artificial host |
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