CN110115251A - A method of extending drosophila class pest parasitic wasp shelf life - Google Patents
A method of extending drosophila class pest parasitic wasp shelf life Download PDFInfo
- Publication number
- CN110115251A CN110115251A CN201910447733.2A CN201910447733A CN110115251A CN 110115251 A CN110115251 A CN 110115251A CN 201910447733 A CN201910447733 A CN 201910447733A CN 110115251 A CN110115251 A CN 110115251A
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- CN
- China
- Prior art keywords
- serphid
- clavicorn
- drosophila
- hair angle
- clavicorn serphid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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- 241000255581 Drosophila <fruit fly, genus> Species 0.000 title claims abstract description 31
- 238000000034 method Methods 0.000 title claims abstract description 25
- 230000003071 parasitic effect Effects 0.000 title claims abstract description 18
- 241000607479 Yersinia pestis Species 0.000 title claims abstract description 14
- 238000011282 treatment Methods 0.000 claims abstract description 31
- 241000382353 Pupa Species 0.000 claims abstract description 21
- 238000004321 preservation Methods 0.000 claims abstract description 12
- 241000256844 Apis mellifera Species 0.000 claims abstract description 10
- 230000013011 mating Effects 0.000 claims abstract description 4
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims description 12
- 229910002092 carbon dioxide Inorganic materials 0.000 claims description 6
- 239000001569 carbon dioxide Substances 0.000 claims description 6
- 238000011081 inoculation Methods 0.000 claims description 6
- 230000024241 parasitism Effects 0.000 claims description 4
- 238000005138 cryopreservation Methods 0.000 claims description 3
- 238000005057 refrigeration Methods 0.000 claims 1
- 229920006395 saturated elastomer Polymers 0.000 claims 1
- 230000032258 transport Effects 0.000 abstract description 6
- 239000000463 material Substances 0.000 abstract description 3
- 210000001015 abdomen Anatomy 0.000 description 4
- 235000013399 edible fruits Nutrition 0.000 description 3
- 235000012055 fruits and vegetables Nutrition 0.000 description 3
- 206010002091 Anaesthesia Diseases 0.000 description 2
- 241000255601 Drosophila melanogaster Species 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 230000037005 anaesthesia Effects 0.000 description 2
- 241000219122 Cucurbita Species 0.000 description 1
- 235000009852 Cucurbita pepo Nutrition 0.000 description 1
- 241001263559 Cynipidae Species 0.000 description 1
- 241000179490 Diapriidae Species 0.000 description 1
- 241001136566 Drosophila suzukii Species 0.000 description 1
- 206010018498 Goitre Diseases 0.000 description 1
- 241000893913 Leptopilina boulardi Species 0.000 description 1
- 241000893917 Leptopilina heterotoma Species 0.000 description 1
- 241000122781 Trichopria drosophilae Species 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 235000021028 berry Nutrition 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000003628 erosive effect Effects 0.000 description 1
- 201000003872 goiter Diseases 0.000 description 1
- 239000000178 monomer Substances 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 230000029305 taxis Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Peptides Or Proteins (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention relates to agricultural pests field of biological control, it is desirable to provide a method of extend drosophila class pest parasitic wasp shelf life.It include: female, the male hair angle clavicorn serphid chosen and just sprouted wings and after sufficiently mating 3-4 days, or still in the intracorporal hair angle clavicorn serphid larva for being developed to 3 ages or more of host drosophila or pupa；Chilling treatment object is placed in 4 DEG C of low temperature environment, carries out chilling treatment and preservation, the holding time is less than or equal to 10 days.The present invention carries out 4 DEG C of low-temperature treatments by the hair angle clavicorn serphid to different growth and development periods, stagnates its growth and development using low temperature, to delay its emergence, extends the holding time.Solution is provided for the preservation transportation problem of hair angle clavicorn serphid investment in actual use；Be conducive to preservation transport when mao angle clavicorn serphid is mass produced, lay a good foundation for its large scale investment actual use.There is reference role to other preservation transports as the honeybee kind of biological control material.
Description
Technical field
The present invention relates to agricultural pests field of biological control, in particular to a kind of extension drosophila class pest parasitic wasp shelf
The method of phase.
Background technique
Drosophila melanogaster Drosophila melanogaster feeding habits are extensive, and growing environment is special, like dark moist inhabit
Place has obvious taxis to sour-sweet and corrupt smell, and the succulence organ of can cause harm a variety of gourd, fruit and vegetables and many plants is weight
The agricultural pests wanted.In recent years, drosophila constantly expands the harm of fruits and vegetables, has spread to Europe, North America, South America etc. at present
A countries and regions more than 30.Spotted wing drosophila Drosophila suzukii is a kind of global pest of moccasin fruit of causing harm, right
Influence caused by fruits and vegetables industry is particularly acute, and is arranged by multiple countries such as the U.S., Japan, Australia, New Zealand, Britain, Germany
For important quarantine pest.Due to berries fruit not easy cleaning, using will be direct after traditional chemical prevention spraying pesticide
Fruit safety is influenced, to influence human health.And conventional control method is difficult to prevent and treat, and is easily destroyed ecological environment, no
Conducive to agricultural sustainable development, thus biological control is particularly important.
Hair angle clavicorn serphid Trichopria drosophilae Perkins, belongs to Hymenoptera Diapriidae, is drosophila
The main parasitic bee in pupa time, host range is wide.In laboratory conditions, parasitic efficiency is higher than ring abdomen cynipidae Figitida two
The main drosophila larvae parasitic wasp of kind --- the small ring abdomen cynipid Leptopilina boulardi of Bu Ladi and the small ring abdomen goitre of different abdomen
Bee Leptopilina heterotoma is preferable biological and ecological methods to prevent plant disease, pests, and erosion resource.
It is thin that the Chinese patent of application number CN201710749534.8 provides a kind of effectively progress artificial propagation hair angle clavicorn
The method of bee obtains good hair angle clavicorn serphid for batch and provides method.And if necessary to using hair angle clavicorn serphid as
If a kind of the biological control material and commodity of large-scale use, the problem of transport and save etc. will be related to.But by
It is difficult to maintain its survival condition in transport and preservation after biological living emergence, causes the commodity shelf phase very short.
Summary of the invention
The technical problem to be solved by the present invention is to overcome deficiency in the prior art, provide a kind of extension drosophila class pest
The method of parasitic wasp shelf life.
In order to solve the above technical problems, solution of the invention is:
There is provided it is a kind of extend drosophila class pest parasitic wasp shelf life method, be choose growth and development period parasitic wasp into
Row chilling treatment, specifically include the following steps:
(1) any one following chilling treatment object are chosen:
(1.1) female, the male hair angle clavicorn serphid just to have sprouted wings, it is spare after sufficiently being mated 3-4 days；
(1.2) still in the intracorporal hair angle clavicorn serphid larva for being developed to 3 ages or more of host drosophila；
(1.3) still intracorporal in host drosophila, it is developed to the hair angle clavicorn serphid in pupa time；
(2) chilling treatment object is placed in 4 DEG C of low temperature environment, carries out chilling treatment and preservation, holding time is small
In equal to 10 days (specific holding time length is adjusted according to the practical needs for saving or transporting).
In the present invention, this method further comprises that the chilling treatment object through cryo-conservation is placed on room temperature (such as 25
DEG C) in environment, chilling treatment object is made to restore normal growth development.
In the present invention, the chilling treatment object is obtained by following methods:
(a) to hair angle clavicorn serphid carry out artificial propagation, including collect and expand numerous honeybee kind, host drosophila inoculation reproduction,
Hair angle clavicorn serphid inoculation, hair angle clavicorn serphid culture with collect, reserve seed for planting (application reference number be CN201710749534.8 in
State's patent)；
(b) every to anaesthetize using a carbon dioxide gas for 24 hours and remove training when close to the emergence period of hair angle clavicorn serphid
Support the drosophila adult in bottle；After hair angle clavicorn serphid sprouts wings, per the adult that will newly be sprouted wings using a carbon dioxide gas for 24 hours
Anesthesia；The adult newly to sprout wings is chosen by the female-male proportion of 2:1, is placed in the incubator of 25 ± 1 DEG C of temperature, humidity 50%, fills
It is spare after dividing mating 3 days；Alternatively,
The drosophila pupa that clavicorn serphid parasitism in coat angle is crossed is chosen, is placed in the incubator of 25 ± 1 DEG C of temperature, humidity 50%
8 days or more, the larva in 3 ages or more is budded into pin main body to hair angle clavicorn serphid；Alternatively,
It is chosen at the hair angle clavicorn serphid for being developed to pupa in pin main body.
In the present invention, when the larva of chilling treatment Object Selection hair angle clavicorn serphid or pupa, be by larva or pupa together with
Its host drosophila carries out chilling treatment and preservation together.
Compared with prior art, the beneficial effects of the present invention are:
1, the present invention is to carry out 4 DEG C of low-temperature treatments by the hair angle clavicorn serphid to different growth and development periods, and utilization is low
Temperature stagnates its growth and development, to delay its emergence, extends holding time (i.e. shelf of the clavicorn serphid in hair angle as commodity
Phase).
2, the preservation transportation problem that the method for the invention is put into actual use for hair angle clavicorn serphid provides solution
Scheme；Be conducive to preservation transport when mao angle clavicorn serphid is mass produced, establish base for its large scale investment actual use
Plinth.
3, the present invention has reference role to other preservation transports as the honeybee kind of biological control material.
Detailed description of the invention
Fig. 1 is the flow chart of the method for the invention.
Specific embodiment
The method of the present invention for extending drosophila class pest parasitic wasp shelf life is to choose posting for proper growth developmental stage
Raw bee carries out chilling treatment, specifically include the following steps:
(1) any one following chilling treatment object are chosen:
(1.1) female, the male hair angle clavicorn serphid just to have sprouted wings, it is spare after sufficiently being mated 3-4 days；
(1.2) still in the intracorporal hair angle clavicorn serphid larva for being developed to 3 ages or more of host drosophila；
(1.3) still intracorporal in host drosophila, it is developed to the hair angle clavicorn serphid in pupa time；
The chilling treatment object is obtained by following methods:
(a) to hair angle clavicorn serphid carry out artificial propagation, including collect and expand numerous honeybee kind, host drosophila inoculation reproduction,
Hair angle clavicorn serphid inoculation, hair angle clavicorn serphid culture with collect, reserve seed for planting (application reference number be CN201710749534.8 in
State's patent)；
(b) every to anaesthetize using a carbon dioxide gas for 24 hours and remove training when close to the emergence period of hair angle clavicorn serphid
Support the drosophila adult in bottle；After hair angle clavicorn serphid sprouts wings, per the adult that will newly be sprouted wings using a carbon dioxide gas for 24 hours
Anesthesia；The adult newly to sprout wings is chosen by the female-male proportion of 2:1, is placed in the incubator of 25 ± 1 DEG C of temperature, humidity 50%, fills
It is spare after dividing mating 3 days；Alternatively,
The female hair angle clavicorn serphid sufficiently to be mated with 3-8 days after emergence with the parasitic 8 age in days drosophila pupas of 1:6 or so ratio,
Take out female hair angle clavicorn serphid afterwards for 24 hours to prevent superparasitization.The drosophila pupa for taking coat angle clavicorn serphid parasitism to cross, is placed on temperature
25 ± 1 DEG C, 8 days or more in the incubator (being purchased from Hangzhou Lai Bo Biotechnology Co., Ltd) of humidity 50%, hair angle hammer at this time
Angle serphid is generally in 3 instar larvaes to the last stage of sprouting wings；Alternatively,
It is chosen at the hair angle clavicorn serphid for being developed to pupa in pin main body.
(2) chilling treatment object is fitted into plastic culture bottle, is placed on 4 DEG C of low temperature environment and carries out chilling treatment and guarantor
It deposits.Holding time is less than or equal to 10 days, and specific holding time length is adjusted according to the practical needs for saving or transporting).
(3) the chilling treatment object through cryo-conservation is moved into the incubator of normal temperature environment (25 ± 1 DEG C of temperature, humidity
50%) chilling treatment object, is made to restore normal growth development.
It is by larva or pupa together with its host drosophila when the larva of chilling treatment Object Selection hair angle clavicorn serphid or pupa
It carries out chilling treatment together and saves (that is, be admitted to low temperature environment is larva or pupa in parasitism, and cannot be point
Separate out the monomer come).
Experimental data and analysis:
Compared with the hair angle clavicorn serphid of the normal growth and development before handling through the invention, hair angle processed by the invention
Clavicorn serphid has no in parasitic rate and in terms of producing female rate and is substantially reduced although emergence time is delayed.In 4 DEG C of low-temperature treatments of longest
Under duration (adult 10 days, larva or pupa 10 days), obtained hair angle clavicorn serphid is to the parasitic rate of Drosophila melanogaster pupa and produces female rate
Respectively 78.75% ± 13.09%, 61.59% ± 17.13% and 85% ± 7.07%, 72.23 ± 7.86%, with without
The individual of processing is compared to there was no significant difference.
The present invention treated offspring individual parasitic rate 80.48% ± 13.11% that hair angle clavicorn serphid given birth to, and just
The hair angle clavicorn serphid of development is frequently grown compared to also no significant difference.
Therefore, produced while emergence time extends through the method for the invention treated hair angle clavicorn serphid female rate,
Parasitic rate and filial generation parasitic rate remain to the higher level for being maintained close to normal growth and development individual without being substantially reduced.
Claims (4)
1. a kind of method for extending drosophila class pest parasitic wasp shelf life, which is characterized in that be to choose posting for growth and development period
Raw bee carries out chilling treatment, specifically include the following steps:
(1) any one following chilling treatment object are chosen:
(1.1) female, the male hair angle clavicorn serphid just to have sprouted wings, it is spare after sufficiently being mated 3-4 days；
(1.2) still intracorporal in host drosophila, it is developed to the hair angle clavicorn serphid larva in 3 ages or more；
(1.3) still intracorporal in host drosophila, it is developed to the hair angle clavicorn serphid in pupa time；
(2) chilling treatment object is placed in 4 DEG C of low temperature environment, carries out chilling treatment and preservation, the holding time is less than etc.
In 10 days.
2. the method according to claim 1, wherein this method further comprises, by the refrigeration through cryo-conservation
Process object is placed in normal temperature environment, and chilling treatment object is made to restore normal growth development.
3. the method according to claim 1, wherein the chilling treatment object is obtained by following methods:
(a) artificial propagation is carried out to hair angle clavicorn serphid, including collecting and expanding numerous honeybee kind, the inoculation reproduction of host drosophila, Mao Jiao
The inoculation of clavicorn serphid, the culture of hair angle clavicorn serphid and collection are reserved seed for planting；
(b) every to anaesthetize using a carbon dioxide gas for 24 hours and remove culture bottle when close to the emergence period of hair angle clavicorn serphid
Interior drosophila adult；After hair angle clavicorn serphid sprouts wings, per using a carbon dioxide gas that the adult newly to sprout wings is numb for 24 hours
It is liquor-saturated；The adult newly to sprout wings is chosen by the female-male proportion of 2:1, is placed in the incubator of 25 ± 1 DEG C of temperature, humidity 50% together,
It is spare after sufficiently mating 3 days；Alternatively,
The drosophila pupa that clavicorn serphid parasitism in coat angle is crossed is chosen, is placed in the incubator of 25 ± 1 DEG C of temperature, humidity 50% 8 days
More than, the larva in 3 ages or more is budded into pin main body to hair angle clavicorn serphid；Alternatively,
It is chosen at the hair angle clavicorn serphid for being developed to pupa in pin main body.
4. the method into 3 any one according to claim 1, which is characterized in that when chilling treatment Object Selection hair angle clavicorn
It is that larva or pupa are subjected to chilling treatment and preservation together with its host drosophila together when the larva of serphid or pupa.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910447733.2A CN110115251A (en) | 2019-05-27 | 2019-05-27 | A method of extending drosophila class pest parasitic wasp shelf life |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910447733.2A CN110115251A (en) | 2019-05-27 | 2019-05-27 | A method of extending drosophila class pest parasitic wasp shelf life |
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| Publication Number | Publication Date |
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| CN110115251A true CN110115251A (en) | 2019-08-13 |
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|---|---|---|---|
| CN201910447733.2A Pending CN110115251A (en) | 2019-05-27 | 2019-05-27 | A method of extending drosophila class pest parasitic wasp shelf life |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116235825A (en) * | 2023-04-14 | 2023-06-09 | 广东省科学院动物研究所 | Method for prolonging shelf life of egg parasitic wasps |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105794728A (en) * | 2016-04-07 | 2016-07-27 | 云南省农业科学院农业环境资源研究所 | Indoor breeding method for drosophila-suzukii-matsumura advantageous parasitic wasps trichopria drosophilae |
| CN107439491A (en) * | 2017-08-28 | 2017-12-08 | 浙江大学 | Strengthen the pupa parasitic wasp artificial breeding production method of drosophila class biological control effect |
| CN108450418A (en) * | 2018-02-27 | 2018-08-28 | 中国林业科学研究院森林生态环境与保护研究所 | A kind of method of biological control fall webworms |
| CN109169526A (en) * | 2018-09-11 | 2019-01-11 | 浙江大学 | The artificial propagation production method of the anti-jaw cocoon bee of open arms |
-
2019
- 2019-05-27 CN CN201910447733.2A patent/CN110115251A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105794728A (en) * | 2016-04-07 | 2016-07-27 | 云南省农业科学院农业环境资源研究所 | Indoor breeding method for drosophila-suzukii-matsumura advantageous parasitic wasps trichopria drosophilae |
| CN107439491A (en) * | 2017-08-28 | 2017-12-08 | 浙江大学 | Strengthen the pupa parasitic wasp artificial breeding production method of drosophila class biological control effect |
| CN108450418A (en) * | 2018-02-27 | 2018-08-28 | 中国林业科学研究院森林生态环境与保护研究所 | A kind of method of biological control fall webworms |
| CN109169526A (en) * | 2018-09-11 | 2019-01-11 | 浙江大学 | The artificial propagation production method of the anti-jaw cocoon bee of open arms |
Non-Patent Citations (3)
| Title |
|---|
| JIANI CHEN等: "Biocontrol characteristics of the fruit fly pupal parasitoid Trichopria drosophilae (Hymenoptera: Diapriidae) emerging from different hosts", 《SCIENTIFIC REPORTS》 * |
| 丁振麟: "《浙江农业科学论文集》", 29 February 1980, 浙江科学技术出版社 * |
| 李隆术: "《储藏物昆虫和农业螨类研究：李隆术论文选》", 31 October 2000, 新疆科技卫生出版社 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116235825A (en) * | 2023-04-14 | 2023-06-09 | 广东省科学院动物研究所 | Method for prolonging shelf life of egg parasitic wasps |
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Application publication date: 20190813 |