CN116171941A - Application of wheat seedlings in cold storage of trichogramma longsynanthassemblies and cold storage method - Google Patents
Application of wheat seedlings in cold storage of trichogramma longsynanthassemblies and cold storage method Download PDFInfo
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- 241000209140 Triticum Species 0.000 title claims abstract description 86
- 235000021307 Triticum Nutrition 0.000 title claims abstract description 86
- 238000000034 method Methods 0.000 title claims abstract description 74
- 241000256618 Trichogramma Species 0.000 title claims abstract description 68
- 238000003860 storage Methods 0.000 title abstract description 19
- 241001470017 Laodelphax striatella Species 0.000 claims abstract description 70
- 235000013601 eggs Nutrition 0.000 claims abstract description 23
- 238000005057 refrigeration Methods 0.000 claims abstract description 17
- 241000238631 Hexapoda Species 0.000 claims abstract description 8
- 206010003883 azoospermia Diseases 0.000 claims abstract description 4
- 208000008634 oligospermia Diseases 0.000 claims abstract description 4
- 230000036616 oligospermia Effects 0.000 claims abstract description 4
- 231100000528 oligospermia Toxicity 0.000 claims abstract description 4
- 238000005286 illumination Methods 0.000 claims description 18
- 238000011081 inoculation Methods 0.000 claims description 12
- 238000012258 culturing Methods 0.000 claims description 10
- 241001466042 Fulgoromorpha Species 0.000 claims description 7
- 230000007613 environmental effect Effects 0.000 claims description 2
- 230000012447 hatching Effects 0.000 claims description 2
- 238000009395 breeding Methods 0.000 abstract description 7
- 230000001488 breeding effect Effects 0.000 abstract description 7
- 230000004083 survival effect Effects 0.000 abstract description 7
- 239000000969 carrier Substances 0.000 abstract description 6
- 230000007774 longterm Effects 0.000 abstract description 4
- 230000018044 dehydration Effects 0.000 abstract description 3
- 238000006297 dehydration reaction Methods 0.000 abstract description 3
- 241000209094 Oryza Species 0.000 description 23
- 235000007164 Oryza sativa Nutrition 0.000 description 23
- 235000009566 rice Nutrition 0.000 description 23
- 230000000052 comparative effect Effects 0.000 description 16
- 230000003071 parasitic effect Effects 0.000 description 8
- 241001414720 Cicadellidae Species 0.000 description 7
- 241000168525 Haematococcus Species 0.000 description 7
- 230000032669 eclosion Effects 0.000 description 7
- 241000606807 Glaesserella parasuis Species 0.000 description 6
- 230000035558 fertility Effects 0.000 description 5
- 241001498622 Cixius wagneri Species 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 4
- 241001556089 Nilaparvata lugens Species 0.000 description 4
- 241000256680 Trichogrammatidae Species 0.000 description 4
- 238000009341 apiculture Methods 0.000 description 4
- 230000000384 rearing effect Effects 0.000 description 4
- 239000002689 soil Substances 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 241000702632 Rice dwarf virus Species 0.000 description 3
- 241000607479 Yersinia pestis Species 0.000 description 3
- NIXOWILDQLNWCW-UHFFFAOYSA-N acrylic acid group Chemical group C(C=C)(=O)O NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 description 3
- 235000013339 cereals Nutrition 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 238000009331 sowing Methods 0.000 description 3
- 102000002322 Egg Proteins Human genes 0.000 description 2
- 108010000912 Egg Proteins Proteins 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 210000004681 ovum Anatomy 0.000 description 2
- 230000000644 propagated effect Effects 0.000 description 2
- 239000002002 slurry Substances 0.000 description 2
- 210000000225 synapse Anatomy 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 208000009084 Cold Injury Diseases 0.000 description 1
- 241000336797 Eoeurysa flavocapitata Species 0.000 description 1
- 241000358422 Nephotettix cincticeps Species 0.000 description 1
- 241000382353 Pupa Species 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000002595 cold damage Effects 0.000 description 1
- 238000010924 continuous production Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000012364 cultivation method Methods 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000017448 oviposition Effects 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 230000001568 sexual effect Effects 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/90—Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
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Abstract
The invention belongs to the technical field of insect feeding, and particularly relates to application of wheat seedlings in cold-stored long-process trichogramma oligospermia. The invention takes the eggs of the Laodelphax striatellus as hosts of the trichogramma longus and wheat seedlings as spawning carriers of the Laodelphax striatellus. The wheat seedlings have cold resistance, can be in a survival state after long-term refrigeration, and can not cause dehydration death of trichogramma with long-process oligose in spawning carrier tissues under normal temperature after being taken out of a refrigerator. The scheme provided by the invention can greatly prolong the storage time of the trichogramma with long process oligose, improve the survival rate and the emergence rate, improve the bee breeding efficiency and realize large-scale artificial breeding.
Description
Technical Field
The invention belongs to the technical field of insect feeding, and particularly relates to application of wheat seedlings in cold storage of trichogramma longsynanthum and a cold storage method.
Background
The black leafhoppers Nephotettix cincticeps (Fabricius) are one of important pests of rice, and besides the damage caused by directly taking rice, the rice can also transmit the common dwarf disease, yellow dwarf disease and other virus diseases of the rice, so that more serious losses are caused. The rice dwarf virus can replicate and proliferate in the host black-tail leafhopper, and the rice is infected with the rice and healthy rice interactively through the feeding action of the leafhopper, so that the rice dwarf virus is epidemic, and the rice is infected in a large area to reduce the yield.
The trichogramma (Oligositashibuyaeishii) is an egg parasitic wasp of the black tail leafhopper, has high natural parasitic rate and has remarkable control effect on the propagation of the black tail leafhopper. The trichogramma with long-process oligose can be used as a host for large-scale feeding, but the brown planthoppers only eat rice, but the rice is not cold-resistant, and the rice seedlings die after being subjected to cold injury in 7 days at the temperature of 4-12 ℃, and although the trichogramma with long-process oligose can survive for a long time (at least 3 months) at the temperature of 4-12 ℃, the trichogramma with long-process oligose can dehydrate and die after the rice seedlings die along with the spawning carrier plants. Therefore, the use of rice seedlings as spawning carriers is not suitable for cold storage of trichogramma longus.
Disclosure of Invention
In order to solve the problems, the invention provides an application of wheat seedlings in cold storage of trichogramma longsynanthum and a cold storage method. The wheat seedlings are used as spawning carriers of the Laodelphax striatellus so as to be used for refrigerating the trichogramma longus, so that the survival rate and the emergence rate of the trichogramma longus can be improved, the trichogramma longus is produced in a large scale and continuously, and large-scale artificial feeding is realized.
The invention provides an application of wheat seedlings in cold-stored trichogramma longsynanthassemblies.
The invention also provides a refrigeration method of the trichogramma with long synapse and oligose, which comprises the following steps:
placing wheat seedlings into a feeding container, and inoculating the laodelphax striatellus to obtain a feeding container containing laodelphax striatellus eggs;
putting trichogramma with long-process oligose into the feeding container containing the Laodelphax striatellus eggs to obtain wheat seedlings with the trichogramma with long-process oligose;
refrigerating the wheat seedlings with the trichogramma with the long-process oligose; the temperature of the refrigeration is 8-12 ℃.
Preferably, the relative humidity of the refrigeration is 60-80%; the refrigerating photoperiod is that 14 hours of illumination is 10 hours of darkness; the illumination intensity of the refrigerating is 2000Lx.
Preferably, after the plant hoppers are inoculated for 5 days, a feeding container containing plant hopper eggs is obtained;
and placing the trichogramma with the long-process oligose into the feeding container containing the small brown planthopper eggs for 5d to obtain the wheat seedlings with the trichogramma with the long-process oligose.
Preferably, the number ratio of the laodelphax striatellus to the trichogramma longus is 1:1.
preferably, the male-female ratio of the laodelphax striatellus is 1:1.
preferably, the cultivation method of the plant hopper for inoculation comprises the following steps:
placing wheat seedlings into a feeding container, inoculating adult Laodelphax striatellus into the feeding container, feeding the adult Laodelphax striatellus on the wheat seedlings for spawning, and after hatching the Laodelphax striatellus eggs, continuing feeding the adult Laodelphax striatellus on the wheat seedlings to develop into adults, and collecting the adults as the Laodelphax striatellus for inoculation.
Preferably, the feeding container comprises a feeding cage.
Preferably, the wheat seedling culturing method comprises the following steps:
culturing germinated wheat seeds for 30-40 d to obtain the wheat seedlings.
Preferably, the environmental conditions of the culture include: the temperature is 22-26 ℃, the relative humidity is 60-80%, the photoperiod is 12h for illumination and 12h darkness, the illumination intensity is 10000-15000 Lx, the light source adopts red-white-blue LED lamps, and the light intensity ratio of red light, white light and blue light is 1:1:1.
the beneficial effects are that:
the invention provides an application of wheat seedlings in cold-stored trichogramma longsynanthassemblies. The wheat seedling is used as the spawning carrier of the Laodelphax striatellus for refrigerating the Haemophilus parasuis, and the Laodelphax striatellus takes eggs laid after eating the wheat seedling and can be used as a host of the Haemophilus parasuis, so that the Haemophilus parasuis can be propagated and amplified; and the wheat seedlings have cold resistance, can be in a survival state after long-term refrigeration, and can not lose water under normal temperature when taken out of the refrigerator, so that the long-process oligocable trichogramma in the wheat seedling tissue is prevented from dying by dehydration. The scheme provided by the invention can prolong the storage time of the trichogramma longus, improve the survival rate and the emergence rate of the trichogramma longus, improve the bee breeding efficiency and realize large-scale artificial breeding.
Detailed Description
The invention provides an application of wheat seedlings in cold-stored trichogramma longsynanthassemblies.
The wheat seedlings are used as spawning carriers of the Laodelphax striatellus for refrigerating the Haemophilus parasuis, and the breeding of the Haemophilus parasuis takes eggs of the Laodelphax striatellus as hosts; because the wheat seedlings have cold resistance and still survive after long-term refrigeration, the wheat seedlings can not lose water under normal temperature after being taken out of the refrigerator, so that the long-process oligocable trichogramma in the wheat seedling tissues is prevented from dying after dehydration.
The invention also provides a refrigeration method of the trichogramma with long synapse and oligose, which comprises the following steps:
placing wheat seedlings into a feeding container, and inoculating the laodelphax striatellus to obtain a feeding container containing laodelphax striatellus eggs;
putting trichogramma with long-process oligose into the feeding container containing the Laodelphax striatellus eggs to obtain wheat seedlings with the trichogramma with long-process oligose;
refrigerating the wheat seedlings with the trichogramma with the long-process oligose; the temperature of the refrigeration is 8-12 ℃.
The wheat seedling is a spawning carrier of the Laodelphax striatellus. In the present invention, the wheat seedlings are preferably obtained by a culture method comprising the steps of: culturing germinated wheat seeds for 30-40 d to obtain the wheat seedlings. The temperature of the culture according to the invention is preferably 22-26 ℃, and more preferably 24 ℃; the relative humidity of the culture is preferably 60-80%; the photoperiod of the culture is preferably 12 hours of illumination and 12 hours of darkness; the illumination intensity of the culture is 10000-15000 Lx; the cultured light source preferably adopts a red, white and blue LED lamp; the ratio of red to white to blue of the red to white to blue LED lamp is 1:1:1. the method for obtaining the germinated wheat seeds is not strictly required, and the germinated wheat seeds can be obtained by adopting a conventional method in the field.
After the wheat seedlings are obtained, the wheat seedlings are preferably placed into a feeding container, the feeding container is connected with the Laodelphax striatellus adults with the male-female ratio of 1:1, the Laodelphax striatellus feeds on spawning carrier plants to spawn, after the Laodelphax striatellus eggs are hatched, nymphs continue to feed on the carrier plants to develop into adults, and the adults are collected to serve as the Laodelphax striatellus for inoculation. In the invention, the ratio of the number of the oviposition carrier of the Laodelphax striatellus to the number of the inoculated Laodelphax striatellus adults is preferably 1 strain: 1 head meter. In the present invention, the feeding container preferably includes a rearing cage. The length, width and height of the insect cage are preferably 40cm multiplied by 60cm.
After the plant hoppers for inoculation are obtained, the novel wheat seedlings are put into a feeding container, and the plant hoppers for inoculation are inoculated to obtain the feeding container containing the plant hopper eggs. In the invention, the quantity ratio of the wheat seedlings to the plant hoppers for inoculation is preferably 1 plant: 1 head; the ratio of the male to the female of the plant hoppers for inoculation is preferably 1:1. After the laodelphax striatellus is inoculated, the laodelphax striatellus takes the wheat seedling and spawns on the wheat seedling, so that a feeding container containing laodelphax striatellus eggs is obtained.
In the invention, after the plant hopper for inoculation is inoculated for 5d, trichogramma with long-process oligose is inoculated into the feeding container, so that wheat seedlings with the long-process oligose trichogramma are obtained. In the present invention, the feeding container preferably includes a rearing cage. The ratio of the number of the laodelphax striatellus to the number of the long-process oligotrichogramma for inoculation is preferably 1:1, a step of; the ratio of the male to the female of the plant hoppers for inoculation is preferably 1:1. the invention takes the Laodelphax striatellus ovum as a host of the Haematococcus elongatus, and inoculates the Haematococcus elongatus to parasitize the ovum of the Laodelphax striatellus, thereby obtaining the wheat seedling with the Haematococcus elongatus.
After obtaining the wheat seedling with trichogramma longus, the wheat seedling with trichogramma longus is preferably placed in a refrigerator for refrigeration. In the present invention, the temperature of the refrigerator is 8 to 12 ℃, preferably 8 ℃, 11 ℃ or 12 ℃, and more preferably 11 ℃; the relative humidity of the refrigerator is 60-80%, preferably 70%; the photoperiod of the refrigerator is that 14 hours of illumination is 10 hours of darkness; the illumination intensity of the refrigerator is 2000Lx.
According to the invention, the wheat seedlings are sequentially inoculated with the Laodelphax striatellus and the Haematococcus elongatus, and after the Laodelphax striatellus feeds the wheat seedlings, the produced Laodelphax striatellus eggs serve as hosts of the Haematococcus elongatus, so that the Haematococcus elongatus is propagated and amplified, and mass and continuous production of the Haematococcus elongatus can be realized; and because the wheat seedlings have cold resistance and are in a survival state after long-term refrigeration, the wheat seedlings are taken out from the refrigerator and cannot lose water under normal temperature conditions, so that the phenomenon that the long-process oligozoosperms in the tissue of the wheat seedlings are dehydrated and die is avoided, the wheat seedlings with the long-process oligozoosperms in the period from larvae to pupa are placed in the field, the long-process oligozoosperms can automatically emerge into Cheng Feng, and the insect eggs of the black tail leafhoppers are parasitic to control the insect pests of the black tail leafhoppers, and the epidemic of rice dwarf is avoided. According to the invention, wheat seedlings with the trichogramma longus are preserved in a refrigerating manner, the length of the refrigerating time can be determined according to the pest prevention and control requirements, and the embodiment results show that the refrigerating is 60d, the influence on indexes such as the emergence rate, the sex ratio, the spawning quantity and the like of the trichogramma longus is small, the emergence rate of the trichogramma longus can still reach more than 50%, the storage time of the trichogramma longus is greatly prolonged, the survival rate and the emergence rate are improved, the breeding efficiency is improved, the large-scale artificial breeding is realized, and the trichogramma longus is applied to fields for preventing and controlling the black tail leafhoppers.
In order to further illustrate the present invention, the application of wheat seedlings provided by the present invention to cold storage of trichogramma longsynanthassemblies and the cold storage method are described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention.
Example 1
A refrigerating method of trichogramma with long process oligose comprises the following steps:
(1) Planting wheat seedlings: filling rice field soil with a square acrylic plate (length, width and height: 32.5cm, 26.5cm, 10 cm) to a thickness of 5cm, sowing wheat seeds, wherein each plate is 100 grains, and the temperature, humidity and illumination conditions are that the temperature is 24+/-2 ℃, the relative humidity is 60-80%, and the photoperiod is L: d=12: 12. the light source adopts red and white Lan Sanse LED lamp light, and the proportion of red, white and blue is 1:1:1, illuminance 10000Lx, and culturing for 30 days to obtain feed and spawning carrier for Laodelphax striatellus;
(2) Feeding the laodelphax striatellus: placing the wheat seedlings obtained in the step (1) into an insect-raising cage, wherein the insect-raising cage has the following specification: placing 2 trays of wheat seedlings in each cage, inoculating 200 heads of adult Laodelphax striatellus (the male-female ratio is 1:1), and allowing the adult Laodelphax striatellus to take food and spawn; after eggs are hatched, the nymphs continue to feed on wheat seedlings for growth, and after the nymphs emerge as adults, the adults are collected and used as refrigerated hosts;
(3) Parasitic and refrigerated: putting the wheat seedlings obtained in the step (1) into a bee-keeping cage, wherein the specification of the bee-keeping cage is as follows: 40cm x 60cm, and 1 tray of wheat seedlings are placed in each cage; transferring 200 adult Laodelphax striatellus (male-female ratio is 1:1) of the eclosion in the step (2) into the transfer device, allowing the adult Laodelphax striatellus to feed on wheat seedlings for spawning, putting into the trichogramma longus after 5d, inoculating 200 adult Laodelphax striatellus to each cage, and placing the wheat seedlings at the temperature of 11 ℃ and the humidity of 60-80% and the photoperiod L after 5d of parasitic growth: d=14: 10, refrigerating in a refrigerator with an illumination intensity of 2000Lx.
Example 2
A method for refrigerating trichogramma long-process, similar to that of example 1, with the only difference that the temperature of the refrigerating in step (3) is 8 ℃.
Example 3
A method for refrigerating trichogramma long-process, similar to that of example 1, with the only difference that the temperature of the refrigerating in step (3) is 12 ℃.
Comparative example 1
A parasitic method of trichogramma with long process oligospermia comprises the following steps:
(1) Planting wheat seedlings: filling rice field soil with a square acrylic plate (length, width and height: 32.5cm, 26.5cm, 10 cm) to a thickness of 5cm, sowing wheat seeds, wherein each plate is 100 grains, and the temperature, humidity and illumination conditions are that the temperature is 24+/-2 ℃, the relative humidity is 60-80%, and the photoperiod is L: d=12: 12. the light source adopts red and white Lan Sanse LED lamp light, and the proportion of red, white and blue is 1:1:1, illuminance 10000Lx, and culturing for 30 days to obtain feed and spawning carrier for Laodelphax striatellus;
(2) Feeding the laodelphax striatellus: placing the wheat seedlings obtained in the step (1) into an insect-raising cage, wherein the insect-raising cage has the following specification: placing 2 trays of wheat seedlings in each cage, inoculating 200 heads of adult Laodelphax striatellus (the male-female ratio is 1:1), and allowing the adult Laodelphax striatellus to take food and spawn; after eggs are hatched, the nymphs continue to feed on wheat seedlings for growth, and after the nymphs eclosion to adults, the adults are collected and used as hosts;
(3) Parasitizing: putting the wheat seedlings obtained in the step (1) into a bee-keeping cage, wherein the specification of the bee-keeping cage is as follows: 40cm x 60cm, and 1 tray of wheat seedlings are placed in each cage; transferring 200 adult Laodelphax striatellus (male-female ratio is 1:1) of the eclosion in the step (2) into the transfer device, allowing the adult Laodelphax striatellus to feed on wheat seedlings for spawning, putting into the trichogramma longus after 5d, inoculating 200 adult Laodelphax striatellus to each cage, and placing the wheat seedlings at the temperature of 27 ℃ and the humidity of 60-80% and the photoperiod L after 5d of parasitic growth: d=14: 10, light intensity 10000 Lx.
Comparative example 2
A refrigerating method of trichogramma with long process oligose comprises the following steps:
(1) Planting rice seedlings: the paddy soil is filled with an acrylic square plate (length x width x height: 32.5cm x 26.5cm x 10 cm) to a thickness of 5cm, and a proper amount of water is added to stir the soil into slurry and trowelling. Uniformly broadcasting germinated TN1 rice on slurry, sowing 100 grains per disc, placing in insect-raising cage (length x width x height: 40cm x 60 cm), and under the conditions of temperature and humidity illumination of (26+ -2) deg.C, relative humidity of 60-80%, photoperiod L: d=14: 10. the light source adopts a red-white-blue three-color LED lamp, and the ratio of red to white to blue is 1:1:1, culturing for 30 days at the illumination intensity of 10000Lx to obtain rice seedlings for standby, wherein the obtained rice seedlings are used for standby;
(2) Parasitic and refrigerated: putting the rice seedlings obtained in the step (1) into an insect rearing cage, wherein the insect rearing cage has the following specification: placing 2 trays of rice seedlings in each cage, and inoculating 200 heads of brown planthopper adults (the male-female ratio is 1:1) to allow the brown planthopper adults to feed and spawn; placing the trichogramma strain into the long-process oligose trichogramma after 5d, inoculating 200 heads of trichogramma in each cage, and placing the rice seedlings at the temperature of 11 ℃ and the humidity of 60-80% after 5d parasitizing, wherein the photoperiod L: d=14: 10, refrigerating in a refrigerator with an illumination intensity of 2000Lx.
Comparative example 3
A refrigeration method of trichogramma long-process oligose similar to comparative example 2 is only different in that the refrigeration temperature is 8 ℃.
Comparative example 4
A refrigeration method of trichogramma long-process oligose similar to comparative example 2 was only different in that the refrigeration temperature was 12 ℃.
Comparative example 5
A method similar to comparative example 2, the only difference being that the seedlings were left without refrigeration at a temperature of 27℃and a humidity of 60-80%, photoperiod L: d=14: 10, light intensity 10000 Lx.
Test example 1
The emergence rate of the trichogrammatid with long process oligose after the cold storage of 20d, 40d and 60d in examples 1-3 and comparative examples 2-4, the emergence rate of the trichogrammatid with long process oligose under the condition of 27 ℃ in comparative examples 1 and 5, the sex ratio, service life and fertility of the trichogrammatid with long process oligose after the cold storage of 20d, 40 and 60d in examples 1-3, the sex ratio, service life and fertility of the trichogrammatid with long process oligose under the condition of 27 ℃ in comparative example 1, and the results of 3 parallel repeated experiments in each example and comparative example are shown in tables 1-6.
TABLE 1 eclosion rate (%)
Note that: the different lowercase letters indicate the significance of the difference at the 0.05 level, with 1, 2, 3 being the results of 3 parallel replicates, respectively, as follows.
TABLE 2 comparative examples 1 and 5 eclosion rate of trichogramma with Magnomonas long process at 27 ℃ (%)
| Group of | 1 | 2 | 3 | Average value of |
| Comparative example 1 | 89.60 | 90.41 | 89.82 | 89.94±0.24a |
| Comparative example 5 | 89.38 | 89.71 | 89.82 | 89.64±0.13a |
TABLE 3 sex ratio of Haemophilus parasuis (percent female,%)
TABLE 4 Life of Long-process oligozoon trichogramma after 20, 40 and 60d cold storage at different temperatures (Tian)
TABLE 5 fertility (number of offspring) of long process oligozoosperms after 20, 40 and 60d cold storage at different temperatures
Table 6 comparative example 1 sex ratio (percentage of female,%), longevity (day) and fertility (number of offspring) of trichogramma with long process oligospermia at 27℃
As can be seen from tables 1 to 6, the rice seedlings are taken as spawning carriers of brown planthoppers, and the low-temperature preservation of the trichogramma with long process oligose cannot be realized; wheat seedlings are used as spawning carriers of the Laodelphax striatellus, after the Laodelphax striatellus is parasitized, the Laodelphax striatellus is stored for 20-60 d at a low temperature of 8-12 ℃, the Laodelphax striatellus can still eclosion, the cold storage effect at a low temperature of 11 ℃ is optimal, the eclosion rate of the Laodelphax striatellus is still higher than 50%, the service life of the Laodelphax striatellus is less influenced, and the sexual ratio and the fertility are not influenced.
Although the foregoing embodiments have been described in some, but not all, embodiments of the invention, it should be understood that other embodiments may be devised in accordance with the present embodiments without departing from the spirit and scope of the invention.
Claims (10)
1. The application of wheat seedlings in refrigerating trichogramma with long process oligospermia is provided.
2. A refrigerating method of trichogramma with long process oligose is characterized by comprising the following steps:
placing wheat seedlings into a feeding container, and inoculating the laodelphax striatellus to obtain a feeding container containing laodelphax striatellus eggs;
putting trichogramma with long-process oligose into the feeding container containing the Laodelphax striatellus eggs to obtain wheat seedlings with the trichogramma with long-process oligose;
refrigerating the wheat seedlings with the trichogramma with the long-process oligose; the temperature of the refrigeration is 8-12 ℃.
3. A method of refrigeration according to claim 2, wherein said refrigerated relative humidity is from 60 to 80%; the refrigerating photoperiod is that 14 hours of illumination is 10 hours of darkness; the illumination intensity of the refrigerating is 2000Lx.
4. The method for refrigerating according to claim 2, wherein the feeding container containing the eggs of the laodelphax striatellus is obtained after inoculating the laodelphax striatellus for 5 d;
and placing the trichogramma with the long-process oligose into the feeding container containing the small brown planthopper eggs for 5d to obtain the wheat seedlings with the trichogramma with the long-process oligose.
5. The method of claim 2, wherein the ratio of the number of the laodelphax striatellus to the trichogramma longus is 1:1.
6. the method of refrigerating according to claim 2, wherein the ratio of male to female of the laodelphax striatellus is 1:1.
7. the method of refrigerating according to claim 2, wherein the method of culturing the plant hoppers for inoculation comprises the steps of:
placing wheat seedlings into a feeding container, inoculating adult Laodelphax striatellus into the feeding container, feeding the adult Laodelphax striatellus on the wheat seedlings for spawning, and after hatching the Laodelphax striatellus eggs, continuing feeding the adult Laodelphax striatellus on the wheat seedlings to develop into adults, and collecting the adults as the Laodelphax striatellus for inoculation.
8. A method of refrigerating as claimed in claim 2 or claim 7 wherein the feeding container comprises an insect cage.
9. The refrigerating method according to claim 2 or 7, wherein the wheat seedling culturing method comprises the steps of:
culturing germinated wheat seeds for 30-40 d to obtain the wheat seedlings.
10. The method of claim 9, wherein the environmental conditions of the culturing include: the temperature is 22-26 ℃, the relative humidity is 60-80%, the photoperiod is 12h for illumination and 12h darkness, the illumination intensity is 10000-15000 Lx, the light source adopts red-white-blue LED lamps, and the light intensity ratio of red light, white light and blue light is 1:1:1.
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