CN100450347C - Method for promoting Miichthys miiuy sexual gland maturity - Google Patents

Method for promoting Miichthys miiuy sexual gland maturity Download PDF

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Publication number
CN100450347C
CN100450347C CNB2007100670963A CN200710067096A CN100450347C CN 100450347 C CN100450347 C CN 100450347C CN B2007100670963 A CNB2007100670963 A CN B2007100670963A CN 200710067096 A CN200710067096 A CN 200710067096A CN 100450347 C CN100450347 C CN 100450347C
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parent population
fish
day
light application
application time
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CN101015287A (en
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李明云
徐万土
竺浚全
郑根兴
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Gulf Of Ningbo Fisheries Seed Breeding Center
Ningbo University
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Gulf Of Ningbo Fisheries Seed Breeding Center
Ningbo University
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

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Abstract

The invention discloses a method for promoting fish gonad maturity, which employs comprehensive adjustment of water temperature adjustment, light emitting adjustment and nutrition adjustment and technology preventing clit insect. The gonad of fish cultured from late February to early March and adjusted with said comprehensive adjustment matures more than one month earlier than that of in natural condition, and gonad of parent fish cultured in the middle October and adjusted with said comprehensive adjustment is about four months earlier than that of in natural condition, which advances fish breeding time. The fecundity is increase in Zhejiang province, the quality of ovum and sperm is improved, the fertility rate is 62.5%, rate of hatchability is 60.1%, and seeding survival rate is 15%.

Description

A kind of method of Cu Miichthys miiuy sexual gland maturity
Technical field
The present invention relates to the propagation technique of marine fishes, relate in particular to a kind of method of Cu Miichthys miiuy sexual gland maturity.
Background technology
fish (Miichthys miiuy (basilewsky)) is Cheng Hei again, and belong to Perciformes, Sciaenidae fish and belong to, be a kind of important economic fish, the market price is also higher, its fish glue particularly, high-grade especially invigorant, very popular; Because now output is few, thus only in the hotel, the restaurant could Chi Dao the fish, be difficult to Jian on the open market Dao the fish.
The fish is a kind of fish of 4 rheological properties maturations, the fish because of period of its gonadal maturation generally after October, so in the October~December in every year, belong to the fish of breeding autumn and winter its mating season; Because the period of its gonadal maturation is slower, even under the artificial condition of breeding, generally need 40~50 day time to fry is long to 3cm from laying eggs, fry is plunged into the commercial sea like this, and to carry out the time that the net cage large size fingerling cultivates be by the end of December, this moment, 8~10 ℃ of of of of of of the water temperature of seawater had only, the fry of 3cm just seldom or stop it and ingest under cryogenic conditions, its cryophylactic ability also a little less than, most fries are difficult to tide over very long and winter severe cold, the overwintering survival rate of fry is just very low, is lower than 3%; If allow its cultivation that stays within survive the winter, the one, pass through to heat up and labor management, but expense is very high; The 2nd, because indoor density is too high, the manufactured feed of throwing something and feeding, water quality is easily ruined, and fish disease takes place frequently, and the survival rate of fry is not high yet, is lower than 5% yet.The artificial breeding scale of fish needs large size fingerling in batches, reach the environmental condition that the above fry of 5cm just can adapt to variation, and prior art is difficult to cultivate large size fingerling in batches, restricts the industrialization of fish culture.
Summary of the invention
Technical problem to be solved by this invention provides a kind of method of Cu Miichthys miiuy sexual gland maturity, the sexual gland of this method Neng Shi fish is reached maturity ahead of time, thereby Shi fish lays eggs and produces the smart time and also shifts to an earlier date, and after fertilization and hatching cultivation, before arriving the winter of cold, just can cultivate into the fry more than the 5cm.
The present invention solves the problems of the technologies described above the technical scheme that is adopted: a kind of method of Cu Miichthys miiuy sexual gland maturity is characterized in comprising the steps:
The selection of a, parent population:the selection body weight is 2~4kg, and the fish of sexual gland undeveloped mature is a parent population, and male and female pairing in 1:1 is weighed, and puts into through the cultivation pond of sterilization and cultivates;
B, control stimulate the clit worm:every 10~15 days, gather the mixed liquor of iodine and melonworm spirit to described parent population dipping 1 hour with height; The every first quarter moon of also available copper sulphate 0.8-1.2mg/L is splashed once to cultivating in the pond, with the parasitism of control stimulation clit worm; Because of stimulate behind the clit worm parasitism not only can Zao Cheng fish malnutrition, and influence its fish brood amount and ovum quality and sperm quality, and further cause the secondary infection bacteriosis, cause parent population death;
C, comprehensive regulation: described comprehensive regulation comprises the water temperature regulate and control to described cultivation pond, to the illumination regulation and control of described parent population, to the nutrition regulation of described parent population; Described nutrition regulation is that every day at the upper and lower noon, each once threw in bait, the total amount of throwing in described bait every day is 3~5% of a described parent population weight, described bait accounts for fish by green grass or young crops or Huang Zhanyu is main, mixes with clam worm and order fish, adds V again in the described bait of per kilogram A10000IU, V E100 μ g and V C30mg can promote its sexual gland to reach maturity ahead of time by described comprehensive regulation; Clam worm and order fish can promote its gonadal maturation, so the amount of mixing is adjusted according to the needs of maturation time, the fish brood amount of the raun of comprehensive regulation Hou fish is just big, and the quality of ovum of raun and the sperm of milter is good.
Described parent population is put into described cultivation pond at late Febuary~early March to be cultivated, 20 ℃ of of of of of of described water temperature regulate and control is that water temperature is controlled at, it is 12 hours that described illumination is regulated to the every day light application time, cultivates the sexual gland of described parent population of mid or late August and just reaches maturity; So just done sth. in advance 1 wheat harvesting period than the gonadal maturation time of the parent population under the nature, the breeding condition of Zhe Jiang fish is greatly improved, grow seedlings by artificial induced spawning and hatching, make fry avoid disadvantageous winter condition, and the fertilization rate reached of parent population is to 62.5%, the fertilized egg hatching rate reaches 60.1%, the survival rate of hatching back fry reaches 15%, before arriving the winter of cold, fry just can be cultivated more than the 5cm, survival rate during the fry rearing also reaches more than 50%, and the above fry of 5cm in the winter time just can safe overwintering.
Described parent population is put into described cultivation pond in mid-October to be cultivated, 0.3 ℃ of every day of described water temperature regulate and control is that water temperature is to descend, 20 ℃ of of of of 25 ℃ of of of of of of of of of of of of from of of make described water temperature drop to, keep described water temperature constant then, described illumination is regulated to and improves light application time 12min every day, make described light application time rise to 12 hours from 9 hours, keep described light application time constant then to by the end of November, then improve described light application time 16min every day again, after making described light application time rise to 16 hours, keep described light application time constant to mid-December, described light application time 10min then descends every day, invariable after making described light application time drop to 14 hours, cultivate the sexual gland of described parent population at the beginning of 6 months and just reach maturity; So just done sth. in advance about 4 months, the breeding condition of Zhe Jiang fish is greatly improved than the gonadal maturation time of the parent population under the nature.
Carry out artificial induced spawning for the parent population oxytocin injection of gonadal maturation, the injection volume of female parent population is 0.5~1.5 μ g/kg, and the injection volume of milter parent population is half of injection volume of female parent population, allows its natural insemination then, obtains fertilized egg; Make incubating oosperm and cultivate into fry with the conventional incubation seedling growing process, cultivate the fry total length and reach 5cm, just can put in a suitable place to breed and cultivate in the afloat net cage or put in a suitable place to breed naturally.
Compared with prior art, the invention has the advantages that owing to pass through water temperature regulate and control, the comprehensive regulation technology of illumination regulation and control and nutrition regulation three combinations and control stimulate the sexual gland of the technology Cu Shi fish of clit worm to reach maturity ahead of time, as parent population the late Febuary~early March is cultivated and comprehensive regulation has just been done sth. in advance 1 wheat harvesting period than the time of the gonadal maturation of De fish Xia the nature, done sth. in advance in the breeding time that cultivate mid-October and comprehensive regulation has just been done sth. in advance about 4 months Shi fishes than the time of the gonadal maturation of De fish Xia the nature as parent population, thereby its breeding condition is greatly improved; And the fish brood amount through Zhe Jiang fish after the method for the present invention increases, ovum quality and sperm quality are improved, make its fertilization rate reached to 62.5%, incubation rate reaches 60.1%, the survival rate of fry reaches 15%, the large size fingerling cultivating survival rate reaches more than 50%, is more than ten octuple of the natural propagation weed survival rate of breeding season.
Embodiment
Below in conjunction with embodiment the present invention is described in further detail.
Embodiment 1
A kind of method of Cu Miichthys miiuy sexual gland maturity comprises the steps:
The selection of a, parent population:in late Febuary, the selection body weight is 2~4kg, and the age is that river, fully-developed Zhejiang, the sexual gland end fish of 3 full ages is a parent population, totally 5 groups of male and female pairings in 1:1, and the title gross weight is 31.2kg, parent population is put into through the cultivation pond of sterilization cultivate then;
B, control stimulate the clit worm: every 10 days, to parent population dipping 1 hour, stimulate the parasitism of clit worm with the high mixed liquor that gathers iodine and melonworm spirit with control;
C, comprehensive regulation: the water temperature of cultivating the pond is controlled at 20 ℃, and every day, light application time was 12 hours, and every morning 8 selects, 4 thirty of afternoon, each once threw in bait, the total amount of throwing in bait every day is 1kg, bait accounts for fish by green grass or young crops or Huang Zhanyu is main, mixes with clam worm and order fish, adds V in per kilogram bait A10000IU, V E100 μ g and V C30mg, like this parent fish rearing to mid-August its sexual gland just reach maturity, as the method parent fish rearing of at the beginning of 3 months, carrying out short Miichthys miiuy sexual gland maturity of the present invention to late August its sexual gland just reach maturity.
Carry out artificial induced spawning for the parent population oxytocin injection of gonadal maturation, the injection volume of female parent population is 0.8 μ g/kg, and the injection volume of milter parent population is half of injection volume of female parent population, allows its natural insemination then, obtain fertilized egg, just obtain grain surplus the fertilized egg 7,250,000; Make incubating oosperm and cultivate into fry with the conventional incubation seedling growing process, the hatching tail surplus the fish 4,360,000 of emerging is cultivated the fry total length and is reached 5cm, and the quantity of fry is 32.9 ten thousand tails, just can put in the afloat net cage cultivation in a suitable place to breed or puts in a suitable place to breed naturally.
Embodiment 2
A kind of method of Cu Miichthys miiuy sexual gland maturity comprises the steps:
The selection of a, parent population:in mid-October, the selection body weight is 2~4kg, and the age is that river, fully-developed Zhejiang, the sexual gland end fish in 3 ages is a parent population, totally 1 group of male and female pairing in 1:1, and the title gross weight is 6.6kg, parent population is put into through the cultivation pond of sterilization cultivate then;
B, control stimulate the clit worm: every 15 days, the mixed liquor of using high poly-iodine and melonworm spirit was to parent population dipping 1 hour, with the parasitism of control clit worm;
C, comprehensive regulation: the water temperature of cultivating the pond is from cultivating 25 ℃ of beginning to descend 0.3 ℃ every day, make water temperature drop to 20 ℃, keep 20 ℃ of water temperatures constant then, light application time is shone beginning in 9 hours from the every day of cultivating beginning, improve light application time 12min every day, make light application time rise to 12 hours, keep constant the arriving by the end of November of light application time 12 hours every days, then to improve light application time 16min every day, after making light application time rise to 16 hours, keep 16 hours every days light application times constant to mid-December, again with the light application time 10min that descends every day, invariable after making light application time drop to 14 hours; Every morning 8 selects, 4 thirty of afternoon each once throw in bait, the total amount of throwing in bait every day is 0.48kg, bait accounts for fish by green grass or young crops or Huang Zhanyu is main, mixes with clam worm and order fish, adds V in per kilogram bait A10000IU, V E100 μ g and V C30mg, its sexual gland is just reached maturity at the beginning of the parent fish rearing to 6 month like this.
Carry out artificial induced spawning for the parent population oxytocin injection of gonadal maturation, the injection volume of female parent population is 1.2 μ g/kg, and the injection volume of milter parent population is half of injection volume of female parent population, allows its natural insemination then, obtains more than 60 ten thousand in fertilized egg; Make incubating oosperm and cultivate into fry with the conventional incubation seedling growing process, the hatching tail surplus the fish 360,000 of emerging is cultivated the fry total length and is reached 5cm, and the quantity of fry is 2.8 ten thousand tails, just can put in the afloat net cage cultivation in a suitable place to breed or puts in a suitable place to breed naturally.

Claims (2)

1, a kind of method of Cu Miichthys miiuy sexual gland maturity is characterized in that comprising the steps:
The selection of a, parent population:the selection body weight is 2~4kg, and it is parent population that ripe fish is grown at the sexual gland end, and male and female pairing in 1:1 is weighed, and puts into through the cultivation pond of sterilization and cultivates;
B, control stimulate the clit worm: every 10~15 days, to described parent population dipping 1 hour, stimulate the parasitism of clit worm with the high mixed liquor that gathers iodine and melonworm spirit with control;
C, comprehensive regulation: described comprehensive regulation comprises the water temperature regulate and control to described cultivation pond, illumination regulation and control to described parent population, nutrition regulation to described parent population, described nutrition regulation is that every day at the upper and lower noon, each once threw in bait, the total amount of throwing in described bait every day is 3~5% of a described parent population weight, described bait accounts for fish by green grass or young crops or Huang Zhanyu is main, mixes with clam worm and order fish, adds V in the described bait of per kilogram A10000IU, V E100 μ g and V C30mg can promote its sexual gland to reach maturity ahead of time by described comprehensive regulation;
D, described parent population is put into described cultivation pond at late Febuary~early March cultivate, described water temperature regulate and control is that water temperature is controlled at 20 ℃, it is 12 hours that described illumination is regulated to the every day light application time, cultivates the sexual gland of described parent population of mid or late August and just reaches maturity.
2, a kind of method of Cu Miichthys miiuy sexual gland maturity is characterized in that comprising the steps:
The selection of a, parent population:the selection body weight is 2~4kg, and it is parent population that ripe fish is grown at the sexual gland end, and male and female pairing in 1:1 is weighed, and puts into through the cultivation pond of sterilization and cultivates;
B, control stimulate the clit worm: every 10~15 days, to described parent population dipping 1 hour, stimulate the parasitism of clit worm with the high mixed liquor that gathers iodine and melonworm spirit with control;
C, comprehensive regulation: described comprehensive regulation comprises the water temperature regulate and control to described cultivation pond, illumination regulation and control to described parent population, nutrition regulation to described parent population, described nutrition regulation is that every day at the upper and lower noon, each once threw in bait, the total amount of throwing in described bait every day is 3~5% of a described parent population weight, described bait accounts for fish by green grass or young crops or Huang Zhanyu is main, mixes with clam worm and order fish, adds V in the described bait of per kilogram A10000IU, V E100 μ g and V C30mg can promote its sexual gland to reach maturity ahead of time by described comprehensive regulation;
D, described parent population is put into described cultivation pond in mid-October to be cultivated, described water temperature regulate and control is that water temperature is to descend 0.3 ℃ every day, make described water temperature drop to 20 ℃ from 25 ℃, keep described water temperature constant then, described illumination is regulated to and improves light application time 12min every day, make described light application time rise to 12 hours from 9 hours, keep described light application time constant then to by the end of November, then improve described light application time 16min every day again, after making described light application time rise to 16 hours, keep described light application time constant to mid-December, described light application time 10min then descends every day, invariable after making described light application time drop to 14 hours, cultivate the sexual gland of described parent population at the beginning of 6 months and just reach maturity.
CNB2007100670963A 2007-02-09 2007-02-09 Method for promoting Miichthys miiuy sexual gland maturity Expired - Fee Related CN100450347C (en)

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CN110892874A (en) * 2019-12-04 2020-03-20 成都市农林科学院 Artificial cross breeding method for flowers and lips
CN112931312B (en) * 2021-02-26 2023-01-03 海南晨海水产有限公司 Artificial breeding method of seriolala quinqueradiata
CN113575474A (en) * 2021-08-03 2021-11-02 安徽农业大学 Artificial temperature-controlled breeding method for acrossocheilus fasciatus
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