CN101933482A - Method for north-south hybridization and reproduction regulation breeding of Lateolabrax - Google Patents

Method for north-south hybridization and reproduction regulation breeding of Lateolabrax Download PDF

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Publication number
CN101933482A
CN101933482A CN2010102895431A CN201010289543A CN101933482A CN 101933482 A CN101933482 A CN 101933482A CN 2010102895431 A CN2010102895431 A CN 2010102895431A CN 201010289543 A CN201010289543 A CN 201010289543A CN 101933482 A CN101933482 A CN 101933482A
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China
Prior art keywords
parent
lateolabrax
perch
breeding
water
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Pending
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CN2010102895431A
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Chinese (zh)
Inventor
方秀
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FUJIAN MINWELL AQUATIC PRODUCTS INDUSTRIAL Co Ltd
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FUJIAN MINWELL AQUATIC PRODUCTS INDUSTRIAL Co Ltd
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Priority to CN2010102895431A priority Critical patent/CN101933482A/en
Publication of CN101933482A publication Critical patent/CN101933482A/en
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    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81—Aquaculture, e.g. of fish

Abstract

The invention discloses a method for north-south hybridization and reproductive regulation breeding of Lateolabrax, which relates to the biotechnology breeding of Lateolabrax. The method comprises the following steps of: (1) taking a standby parent fish bred from the northern Lateolabrax seed as the male parent and a standby parent fish bred from the local wild Lateolabrax seed as the female parent; (2) breeding the male and the female parents to about 1500g to be used as the parent fishes; (3) artificially inducing the Lateolabrax through a reproductive regulation: controlling the time and the number of hatching of the Lateolabrax parent fishes by means of temperature, photoperiod and hormone injection, controlling the illumination time to 8h, reducing the temperature by 0.3 DEG C every day via a water cooler, and injecting CRH-A3 to the dorsal muscles of the parent fishes after anesthesia with allylguaiacol when the water temperature is reduced to 22 DEG C to induce the parent fishes to spawn, wherein the reproductive regulation induction period of the Lateolabrax is 7 days, and the Lateolabrax can be induced for several periods; (4) breeding the germ cells of the Lateolabrax; and (5) breeding juvenile fishes and breeding the juvenile fishes on a lower row when the sizes of the juvenile fishes reach more than 5cm. The invention has the advantage of breeding the new Lateolabrax seed which has the advantages of high temperature resistance, high growth speed, strong disease resistance and good quality by hybridizing northern and southern Lateolabraxes.

Description

North and south hybridization of flower perch and genital regulating selection
Technical field:
The present invention relates to the biological breeding technique of flower perch, particularly spend the selection of perch new lines.
Background technology:
Flower perch tradition selection is at present:
1, the parent population source is selected: the colored perch finished product fish of local marine cage culture;
2, select before parent fish rearing and the artificial induced spawning: select physique stalwartness, build color and luster good, have that canonical biometric is learned feature and body weight surpasses the colored perch of 1500g as parent population;
3, artificial induced spawning: parent population is row dorsal muscles injection CRH-A after eugenol anesthesia 3Induce parent population to lay eggs, the back parent population of hastening parturition is stored up in nursery pond by (♀: ♂=3: 1), stimulation by running water, and parent population lays eggs and arranges essence, collects fertilized egg;
4, spend the perch incubating oosperm: adopt the spilling water method to collect fertilized egg, weighing counting back is by 4 * 10 4Grain/m 3～5 * 10 4Grain/m 3Move into directly hatching in the ovum hatchery, hatchery day quantity of exchanged water 120～150%, every 2m 3Water body is laid 1 in gas stone, continuous little qi of chong channel ascending adversely, 20～22 ℃ of control water temperatures, salinity 22～25%;
5, larvae juvenile fish is cultivated: inject clean sea water in cultivating the pond, press 35-45 ten thousand tails/m 3Put into colored perch prelarva, 1.5m is pressed in inflation 3Water body is laid 1 in gas stone, and cultivate little qi of chong channel ascending adversely early stage, along with young postlarva development growth strengthens aeration quantity gradually.Physical and chemical factor: 16～22 ℃ of water temperatures, salinity 24～27%, PH8.1～8.6, DO6-8mg/L, COD＜10mg/L; Bait throwing in: the young postlarva stage drops into and scratches sufficient class, and the juvenile fish stage is dropped into mixed bait;
Treat that the juvenile fish specification reaches more than the 5cm, following row cultures.
Defective is: this no specific optionally seedling-cultivating method, can't overcome colored perch close relative's defective of breeding for many generations, and make a little less than the fry physique that traditional seedling-cultivating method produces.Thereby disease slowly, easily takes place in growth, causes that output reduces, benefit descends.
Summary of the invention:
The objective of the invention is to overcome above-mentioned defective, hybridization of a kind of colored perch north and south and genital regulating selection are provided.
The solution of the present invention is:
The selection step is:
1, the parent population source is selected: the parent population male parent is the reserve parent fish of north flower perch seed rearing, and female parent is the reserve parent fish of local wild flowers perch seed rearing;
2, select before parent fish rearing and the selection artificial induced spawning: reserve parent fish is taken pains to foster by 200 tails/case (♀ 140 tails, ♂ 60 tails) density in seawater cage, cultivate about 1500 grams, as parent population;
3, spend perch genital regulating artificial induced spawning: by genital regulating means such as temperature, photoperiod and injection of hormone, thus control flower perch parent population egg-laying time and number of times;
Control measures are specially: at the beginning of late August～12 month, carry out temperature, photoperiod and hormone regulating and controlling, late August moves into the indoor rearing seedling room with parent population, control light application time every day and be 8 hours (8:00～16:00), lower the temperature 0.3 ℃ every day with water chiller, when water temperature was reduced to 22 ℃, parent population is row dorsal muscles injection CRH-A after eugenol anesthesia 3Induce parent population to lay eggs, the back parent population of hastening parturition is stored up in nursery pond stimulation by running water by (♀ 140 tails, ♂ 60 tails), parent population lays eggs and arranges essence, collects fertilized egg, and the flower perch genital regulating cycle of hastening parturition is 7 days, but the multicycle hastens parturition, and hasten parturition time and number of times are controlled as stated above;
4, spend the perch incubating oosperm: adopt the spilling water method to collect fertilized egg, weighing counting back is by 4 * 10 4Grain/m 3～5 * 10 4Grain/m 3Move into directly hatching in the ovum hatchery, hatchery day quantity of exchanged water 120～150%, every 2m 3Water body is laid 1 in gas stone, continuous little qi of chong channel ascending adversely, 20～22 ℃ of control water temperatures, salinity 22～25%;
5, larvae juvenile fish is cultivated: inject clean sea water in cultivating the pond, by 35～450,000 tails/m 3Put into colored perch prelarva, 1.5m is pressed in inflation 3Water body is laid 1 in gas stone, and cultivate little qi of chong channel ascending adversely early stage, along with young postlarva development growth strengthens aeration quantity, physical and chemical factor gradually: 16～22 ℃ of water temperatures, salinity 24～27%, PH8.1～8.6, DO6-8mg/L, COD＜10mg/L; Bait throwing in: the young postlarva stage drops into and scratches sufficient class, and the juvenile fish stage is dropped into mixed bait;
Treat that the juvenile fish specification reaches more than the 5cm, following row cultures.
The invention has the advantages that: utilize fast growth, the strong north of resistance against diseases flower perch and high temperature resistant, the bodily form is slender, the south flower perch that meat is good hybridizes, cultivate the colored perch seed that high temperature resistant, fast growth, resistance against diseases reach quality better by force and culture this new lines flower perch seed, help improving culture benefit and perch quality, strengthen the perch product market competitiveness.
Embodiment:
Selection step of the present invention is:
1, the parent population source is selected;
2, select before parent fish rearing and the artificial induced spawning;
3, artificial induced spawning;
4, spend the perch incubating oosperm: adopt the spilling water method to collect fertilized egg, weighing counting back is by 4 * 10 4Grain/m 3～5 * 10 4Grain/m 3Move into directly hatching in the ovum hatchery, hatchery day quantity of exchanged water 120～150%, every 2m 3Water body is laid 1 in gas stone, continuous little qi of chong channel ascending adversely, 20～22 ℃ of control water temperatures, salinity 22～25%;
5, larvae juvenile fish is cultivated: inject clean sea water in cultivating the pond, by 35～450,000 tails/m 3Put into colored perch prelarva, 1.5m is pressed in inflation 3Water body is laid 1 in gas stone, and cultivate little qi of chong channel ascending adversely early stage, along with young postlarva development growth strengthens aeration quantity, physical and chemical factor gradually: 16～22 ℃ of water temperatures, salinity 24～27%, PH8.1～8.6, DO6-8mg/L, COD＜10mg/L; Bait throwing in: the young postlarva stage drops into and scratches sufficient class, and the juvenile fish stage is dropped into mixed bait;
Treat that the juvenile fish specification reaches more than the 5cm, following row cultures.
It is characterized in that:
Step 1. parent population source is chosen as the reserve parent fish of parent population male parent for north flower perch seed rearing, and female parent is the reserve parent fish of local wild flowers perch seed rearing;
2. step is chosen as before parent fish rearing and the artificial induced spawning reserve parent fish is taken pains to foster by 200 tails/case (♀ 140 tails, ♂ 60 tails) density in seawater cage, cultivates about 1500 grams, as parent population;
Step 3. artificial induced spawning is flower perch genital regulating artificial induced spawning: by genital regulating means such as temperature, photoperiod and injection of hormone, thus control flower perch parent population egg-laying time and number of times;
Control measures are specially: at the beginning of late August～12 month, carry out temperature, photoperiod and hormone regulating and controlling, late August moves into the indoor rearing seedling room with parent population, control light application time every day and be 8 hours (8:00～16:00), lower the temperature 0.3 ℃ every day with water chiller, when water temperature was reduced to 22 ℃, parent population is row dorsal muscles injection CRH-A after eugenol anesthesia 3Induce parent population to lay eggs, the back parent population of hastening parturition is stored up in nursery pond by (♀ 140 tails, ♂ 60 tails), stimulation by running water, and parent population lays eggs and arranges essence, collects fertilized egg; Flower is 7 days in the perch genital regulating cycle of hastening parturition, but during at the beginning of late August to 12 month the multicycle hasten parturition, when the down continuous cycle hastens parturition by above-mentioned condition and method control.
Innovative point of the present invention is:
1, north flower perch kind is moved south to and is carried out adaptability reinforcement cultivation, preferentially as the reserve parent fish male parent, fishes for local wild flowers perch and raises and train, and preferentially as the reserve parent fish female parent, utilizes this male parent, maternal parent population to hybridize, and cultivates flower perch seed.
2, by temperature, the control measures such as photoperiod and injection of hormone, artificial induction's Gunther parent fish spawning and ejaculation, manual control spend perch artificial induced spawning number of times and time, the production of growing seedlings of the colored perch of service.

Claims (1)

1. spend hybridization of perch north and south and genital regulating selection, it is characterized in that: the selection step is:
1. the parent population source is selected: the parent population male parent is the reserve parent fish of north flower perch seed rearing, and female parent is the reserve parent fish of local wild flowers perch seed rearing;
2. select before parent fish rearing and the selection artificial induced spawning: reserve parent fish is taken pains to foster by 200 tails/case ♀ 140 tails, ♂ 60 tail density in seawater cage, cultivate about 1500 grams, as parent population;
3. spend perch genital regulating artificial induced spawning: by genital regulating means such as temperature, photoperiod and injection of hormone, thus control flower perch parent population egg-laying time and number of times;
Control measures are specially: at the beginning of late August～12 month, carry out temperature, photoperiod and hormone regulating and controlling, late August moves into the indoor rearing seedling room with parent population, controlling light application time every day is 8～16 hours, lower the temperature 0.3 ℃ every day with water chiller, when water temperature was reduced to 22 ℃, parent population is row dorsal muscles injection CRH-A after eugenol anesthesia 3Induce parent population to lay eggs, the back parent population of hastening parturition is stored up in nursery pond by ♀ 140 tails, ♂ 60 tails, stimulation by running water, the parent population row of laying eggs is smart, collects fertilized egg, the flower perch genital regulating cycle of hastening parturition is 7 days, but the multicycle hasten parturition, hasten parturition time and number of times are controlled as stated above;
4. spend the perch incubating oosperm: adopt the spilling water method to collect fertilized egg, weighing counting back is by 4 * 10 4Grain/m 3～5 * 10 4Grain/m 3Move into directly hatching in the ovum hatchery, hatchery day quantity of exchanged water 120～150%, every 2m 3Water body is laid 1 in gas stone, continuous little qi of chong channel ascending adversely, 20～22 ℃ of control water temperatures, salinity 22～25%;
5. larvae juvenile fish is cultivated: inject clean sea water in cultivating the pond, by 35～450,000 tails/m 3Put into colored perch prelarva, 1.5m is pressed in inflation 3Water body is laid 1 in gas stone, and cultivate little qi of chong channel ascending adversely early stage, along with young postlarva development growth strengthens aeration quantity, physical and chemical factor gradually: 16～22 ℃ of water temperatures, salinity 24～27%, PH8.1～8.6, DO6-8mg/L, COD＜10mg/L; Bait throwing in: the young postlarva stage drops into and scratches sufficient class, and the juvenile fish stage is dropped into mixed bait;
Treat that the juvenile fish specification reaches more than the 5cm, following row cultures.
CN2010102895431A 2010-09-25 2010-09-25 Method for north-south hybridization and reproduction regulation breeding of Lateolabrax Pending CN101933482A (en)

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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103891654A (en) * 2014-04-14 2014-07-02 江苏中洋集团股份有限公司 Artificial breeding method of new species of Murray cods
CN104621016A (en) * 2014-11-17 2015-05-20 苏州市相城区盛胡特种养殖专业合作社 Breeding method for buffalo perches
CN104904637A (en) * 2015-06-11 2015-09-16 刘有文 Method of cultivating fry of Pelteobagrus fulvidraco
CN105379651A (en) * 2015-11-18 2016-03-09 中国海洋大学 Method for salinity domestication of lateolabraxjaponicus fry
CN105494210A (en) * 2016-01-05 2016-04-20 福建闽威实业股份有限公司 New-variety reproductive regulation breeding method of lateolabrax japonicus
CN111165401A (en) * 2020-02-27 2020-05-19 广西壮族自治区水产引育种中心 Rapid and efficient breeding method for tilapia

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1915007A (en) * 2006-09-12 2007-02-21 河北农业大学 Production method for summer offspring of Songjiang blue pickerel
CN101669451A (en) * 2009-06-30 2010-03-17 刘青华 Method for improving production efficiency of yellow perch by hybridization with perca schrenki

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1915007A (en) * 2006-09-12 2007-02-21 河北农业大学 Production method for summer offspring of Songjiang blue pickerel
CN101669451A (en) * 2009-06-30 2010-03-17 刘青华 Method for improving production efficiency of yellow perch by hybridization with perca schrenki

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
《中国水产》 20080531 于清海等 花鲈工厂化人工育苗技术 第56-58页 1 , 第5期 2 *
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Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103891654A (en) * 2014-04-14 2014-07-02 江苏中洋集团股份有限公司 Artificial breeding method of new species of Murray cods
CN103891654B (en) * 2014-04-14 2016-03-23 江苏中洋集团股份有限公司 A kind of artificial fecundation method of new varieties dragon design spot
CN104621016A (en) * 2014-11-17 2015-05-20 苏州市相城区盛胡特种养殖专业合作社 Breeding method for buffalo perches
CN104904637A (en) * 2015-06-11 2015-09-16 刘有文 Method of cultivating fry of Pelteobagrus fulvidraco
CN105379651A (en) * 2015-11-18 2016-03-09 中国海洋大学 Method for salinity domestication of lateolabraxjaponicus fry
CN105494210A (en) * 2016-01-05 2016-04-20 福建闽威实业股份有限公司 New-variety reproductive regulation breeding method of lateolabrax japonicus
CN105494210B (en) * 2016-01-05 2016-08-24 福建闽威实业股份有限公司 Lateolabrax japonicus genital regulating breeding method
CN111165401A (en) * 2020-02-27 2020-05-19 广西壮族自治区水产引育种中心 Rapid and efficient breeding method for tilapia
CN111165401B (en) * 2020-02-27 2023-04-25 广西壮族自治区水产引育种中心 Quick and efficient breeding method for tilapia mossambica

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