CN108056049A - A kind of Micropterus salmoides autumn mating system - Google Patents
A kind of Micropterus salmoides autumn mating system Download PDFInfo
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- 239000003488 releasing hormone Substances 0.000 claims description 2
- 238000010792 warming Methods 0.000 claims description 2
- 102000009151 Luteinizing Hormone Human genes 0.000 claims 2
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- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical group OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims 1
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- 241000723298 Dicentrarchus labrax Species 0.000 description 5
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- 241000269799 Perca fluviatilis Species 0.000 description 3
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- 241000894007 species Species 0.000 description 2
- 241000269817 Centrarchidae Species 0.000 description 1
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- XNOPRXBHLZRZKH-MQYCRUOZSA-N oxytocin Chemical compound C([C@H]1C(=O)N[C@H](C(N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CSSC[C@H](N)C(=O)N1)C(=O)N1C(CCC1)C(=O)N[C@@H](CC(C)C)C(=O)NCC(N)=O)=O)[C@@H](C)CC)C1=CC=C(O)C=C1 XNOPRXBHLZRZKH-MQYCRUOZSA-N 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention discloses a kind of Micropterus salmoides autumn mating systems, and described method includes following steps：1）Juvenile stage；2) cement pit is cultivated；3) low temperature induction；4) nutrition regulation；5) sexual gland conditioning agent is injected；6) parent's reinforced cultivating；7) autumn breeds.The methods of mating system is by taking cement pit cultivation, low temperature induction, nutrition regulation, sexual gland conditioning agent to inject, Micropterus salmoides being capable of 1 year secondary development, sexual gland is reached maturity again then after spring lays eggs, realize autumn breeding, the breeding of Micropterus salmoides autumn is realized for the first time, breaks through Micropterus salmoides autumn raising technology.
Description
Technical field
The invention belongs to fry raising technology field, specifically, being related to a kind of Micropterus salmoides fish autumn mating system.
Background technology
Micropterus salmoides (scientific name：Micropterus salmonides), belong to Perciformes, Sunfishes on taxology.Body prolongs
Long and flat-sided, slightly in spindle, cross section is ellipse.Body is high to be grown with body than being 1:3.5~4.2, long and body length is than being 1:
3.2~3.4.Head is big and long.Eye is big, and eyeball protrudes.Kiss length, mouth is upper, and schistostoma is big and wide.Jawbone, palatine bone, vomer have completely
Comb teeth, it is more and tiny, it is in the same size.It is plump to carry on the back meat.Caudal peduncle is long and high.Whole body drapes over one's shoulders grey silvery white or faint yellow fine and closely woven scale,
But one line color of ridge is deeper, often in malachite color or light black, while nearby often has black speckle along side line.Abdomen is greyish white.
It since Suzhou is low compared to latitude residing for In Guangdong Province, shines upon that the time is few, and total accumulated temperature is few whole year, causes local big
The mouth sea bass speed of growth is slow.Local raiser fry puts the time in a suitable place to breed and concentrates on mid-March to mid-April, and local Micropterus salmoides
The time is bred in early or mid March to by the end of April, culturing time terminates for mid-March to second year August part, and the culture-cycle is 18
Month.The new earliest Time To Market of mouth is October, catches and stays greatly small, is largely focused on 4~August part of second year.Micropterus salmoides pin
General 6~August price highest is sold, price is in falling tendency after September, until second year price rally in May.But in 7~August
Temperature, water temperature are high, and it is very big to sell fish risk, so price preferably and is sold fish and easily weighed within the entire Culture Techniques of Micropterws Salmoides cycle
The conjunction period is simultaneously few, is unfavorable for culture benefit and maximumlly plays.
It is limited by the restriction of both Suzhou climate and seed supply factor, the solution method that can not find at present.But
It is that Micropterus salmoides autumn breeding key technology but can be good at solving the problems, such as this.Micropterus salmoides autumn breeding key technology
Breakthrough can change that current Micropterus salmoides seed supply time is single, and the aquaculture model cycle is long, and marketable fish concentrates listed price low,
Across year listing is of high cost and the high present situation of high temperature marketing risk, is realized for diversification aquaculture model and provides technical support.
Needs are reformed according to National agricultural supply side, using national characteristic fresh-water fishes industrial system construction as opportunity, adjustment is big
Mouth sea bass cultivation industry frame mode carries out Micropterus salmoides autumn breeding key technology research, realizes that enterprise increases income, agriculture fisherman increases
Effect.
Key technology is bred for Micropterus salmoides autumn, it is domestic at present there is no related article and patent.Similar mainly deposits
In following patent document：
Chinese Patent Application No.：201510819067.2 a kind of early breeding method of Micropterus salmoides, including：A, Selection parent
Perch is cultivated early stage cultivating in the pool；B, select it is physical strong, carry out without hindering disease-free parent and be put into transparent heat insulating greenhouse
Reinforced cultivating；C, the artificial fish nest for being attached with fertilized eggs is put into light transmission hatching greenhouse and hatched, it is artificial to control hatchery water temperature.
By the method for the present invention, Micropterus salmoides in the case where artificial induced spawning, artificial insemination need not be carried out, by control water quality,
The conditions such as nutrition, dissolved oxygen, temperature can achieve the purpose that natural spawning, natural fertilization, practice have shown that the present invention is in hatchery
In establish temperature-controlling system, and when controlling 25-28 DEG C of hatchery water temperature, can effectively prevent Micropterus salmoides fertilized eggs infection saprolegniasis,
Substantially increase the hatching survival rate of fertilized eggs.
Chinese Patent Application No.：201510822685.2 a kind of method for promoting Micropterus salmoides Early- breeding, is included in 9
Before month, the perch in 1 age and/or 2 ages is selected to cultivate the parent 1000-1300 for putting 0.5- 0.7kg in a suitable place to breed in the pool per acre as parent
Group；It is daily to feed expanded pellet diet, it is placed with expanded pellet diet centralised arrangement in the cultivation pool；In September to during November, use is chilled
Fish feeds parent population, and when temperature is less than 15 DEG C, Selection parent, which is put into transparent heat insulating greenhouse, carries out reinforced cultivating, opens micro-
Hole oxygen increasing equipment carries out spawning pond bath processing, late March, in spawning pond security protection artificial fish nest.By the method for the present invention,
Micropterus salmoides is in the case where that need not carry out artificial induced spawning, artificial insemination, by controlling the conditions such as water quality, nutrition, temperature
Can achieve the purpose that natural spawning, natural fertilization, in this method each operation Micropterus salmoides Early- breeding is played to
Close important role.
Chinese Patent Application No.：201410013058.X, a kind of method for hatching Micropterus salmoides ahead of time are big in greenhouse cultivation
Mouth sea bass, ahead of time up to sexal maturity, artificial propagation.In mid-October, 0.5-0.8 kilograms of Micropterus salmoides is chosen, cold season exists
It is cultivated in greenhouse；Start to control water temperature from mid-October, in water temperature control in January at 11-13 DEG C, then gradually heating, to March
The middle ten days rises to 20-22 DEG C；Parent population sexal maturity situation is dissected and observed；To parent population oxytocin injection at the beginning of 4 months, the modeling of palm piece is being placed with
Expect it is allowed to lay eggs in the fishpond of greenhouse；Palm piece with fertilized eggs is put into indoor hatchery hatching.As a result：Obtain overall length
3.5 millimeters of 12,810,000 tail of fry.Northern China there are no the report of Micropterus salmoides high-volume artificial incubation at this stage, especially
It is the report for not having hatching in April Micropterus salmoides in northern China.Micropterus salmoides fish can be obtained by shifting to an earlier date 40 days in Efficiency in Buildings in Tianjin Area
Seedling.The growth period of seed then can be made to extend 40 days.
The content of the invention
Above-mentioned to solve the problems, such as, it is an object of the invention to provide a kind of Micropterus salmoides autumn mating system, institutes
State the methods of mating system is by taking cement pit cultivation, low temperature induction, nutrition regulation, sexual gland conditioning agent to inject, Micropterus salmoides
Can 1 year secondary development, spring lay eggs after sexual gland reach maturity again then, realize autumn breeding, realize that big mouth is black for the first time
Perch autumn breeds, and breaks through Micropterus salmoides autumn raising technology.
In order to achieve the above objectives, the technical scheme is that：
Micropterus salmoides autumn mating system, described method includes following steps：
1) Juvenile stage
Spring bosom ovum is drained through the Micropterus salmoides of ovum 4~May, more than specification 0.55kg/ tails, physical strong, body colour is good, nothing
It is lopsided, not damaged, disease-free；
2) cement pit is cultivated
Cement pit sets culvert system, aeration system, refrigeration system, water source is cultivated using lake water or well water, by step
1) parent in is put into cement pit, and breeding density is 10~12kg/ ㎡, and cement pit water temperature is consistent with parent's transport water temperature, parent
It tides over after the laundering period, except water is not changed during disease control and during cooling, cement pit replaces water body 1/5th daily, daily
Soil pick-up removes residual bait excrement, and disease aims at prevention, and parent splashes compound iodine sterilization for continuous two days when just having entered pond, and cement pit is used
Water water quality dissolved oxygen is 8~10mg/L, and pH value is 7~8；
3) low temperature induction
Parent tides over the laundering period, rejuvenates, and after normally ingesting, starts low temperature induction in early August, induces initial temperature
For 28 DEG C, with 7 days for Unit 1, after being progressively down to 14 DEG C, 20 DEG C are warming up to, it is consistent with outdoor water temperature；It keeps afterwards and outdoor water
Warm consistent, water temperature is 18~20 DEG C；
4) nutrition regulation
Bait is fed as the special compound feed of Micropterus salmoides, the morning 8:00th, afternoon 4:00 feeds 2, and feeding volume is
0.5~1wt% of weight；
5) sexual gland conditioning agent is injected
By low temperature induction after injection sexual gland conditioning agent, sexual gland conditioning agent is Malaysia dioxone DOM, promotees corpus luteum life parent
Into hormone-releasing hormone analog LRH-A2, dosage is 1~1.5mg/kg of DOM, LRH-A210~15 μ g/kg, take muscle to note
Penetrate, injection position portion be dorsal fin base portion, frequency injection 1-2 times；
6) parent's reinforced cultivating
Parent is put into spawning pond outside pond after injecting sexual gland conditioning agent, and breeding density is 100~110kg/ mus；
7) autumn breeds
Parent passes through reinforced cultivating, and gonad development situation, 10 last ten-days period ovaries and essence is periodically dissected and observed in sexual gland Rapid development
Nest is reached maturity, parent pond arrangement breeding fish nest, is spaced 1 meter and is placed 1, oviposition, batch are laid eggs, and spawning pond selects plastics to protect
Warm greenhouse or outdoor earth pond, plastic, thermal-insulation greenhouse temperature are controlled at 20~28 DEG C, and 17~20 DEG C of outdoor ponds water temperature will lay eggs
Fish nest is hatched.
Further, the Micropterus salmoides is two ages old mouth Micropterus salmoides.
Preferably, the step 2) parent is cultivated in cement pit workshop, and cement pit specification is long 4m × wide 4m
× depth 1m, water source use lake water or well water；Lake water uses after filter device precipitates mud, filtering organic matter；Well water passes through
It is used after fully sudden and violent gas, oxidation, precipitation.
Preferably, low temperature induction in the step 3), induction initial temperature is 28 DEG C, with 7 days for Unit 1, the drop of Unit the 1st
Warm amplitude is 3.8 DEG C, is down to 24.2 DEG C；2nd 3.7 DEG C of unit cooling extent, is down to 20.5 DEG C；The holding of Unit the 3rd water temperature 20~
21℃；4th 3 DEG C of unit cooling extent, is down to 17 DEG C；Unit the 5th cools down 3 DEG C, is down to 14 DEG C；The holding of Unit the 6th water temperature 14~
15℃；Unit the 7th heats up 2~3 DEG C, rises to 17 DEG C；Unit 8 heats up 3 DEG C, rises to 20 DEG C.
Preferably, injection time is early October in the step 4).
Preferably, nutrition regulation in the step 6) mixes VC, VE, Bei Rui in the special compound feed of Micropterus salmoides
Health, calcium gluconate.
Preferably, the VC, VE, Bei Ruikang, calcium gluconate total amount feed for the special compound feed of Micropterus salmoides
0.5~1wt% of amount
Preferably, step 6) the parent reinforced cultivating, parent are put into spawning pond after injection, and ice is changed to by feedstuff feeding
Fresh fish is fed, every morning 8:00 1, afternoon 4:00 1.
Preferably, in the step 7), October 25 ovary and spermary be developed to the fifth phase, reached maturity, October
Breeding fish nest is arranged in parent pond on the 26th, and oviposition on October 27, November 1, batch was laid eggs, and breeding fish nest was hatched, November 9
Day hatches the 1st batch of 2,050,000 tail of autumn Micropterus salmoides spray, and Micropterus salmoides fertilized eggs 12,840,000 were collected by November 10,
Produce 11,250,000 tail of spray.
The invention has the advantages that：
The method can change current Micropterus salmoides seed supply in the breakthrough of Micropterus salmoides autumn breeding key technology
Time is single, and the culture-cycle is long, and marketable fish concentrates listed price low, and across year listing is of high cost and high temperature marketing risk is high shows
Shape provides technical support for the realization of diversification aquaculture model.It establishes for the first time at home and covers that parent's cement pit is cultivated, low temperature lures
It leads, the Micropterus salmoides autumn breeding key technology such as nutrition regulation, sexual gland conditioning agent, solves Micropterus salmoides marketable fish high temperature season
Market supply required seed supply problem in short supply, it is significant, significant economic benefit and social benefit will be generated；
Carry out thermal stimulus in early August, undergo from high temperature and drop to low temperature, then by low-temperature-rise to high temperature, October 7 Nikkei
Be put into parent's spawning pond reinforced cultivating after injection sexual gland conditioning agent, realize and laid eggs for the first time October 27, November 1 large quantities of volume productions
Ovum, fertilized eggs 12,840,000 are collected by November 10, in, and 11,250,000 tail of production Micropterus salmoides spray realizes Micropterus salmoides for the first time
Autumn breeds, and breaks through Micropterus salmoides autumn raising technology.
Specific embodiment
With reference to specific embodiment, the present invention is described in detail.Following embodiment will be helpful to the technology of this field
Personnel further understand the present invention, but the invention is not limited in any way.It should be pointed out that the ordinary skill to this field
For personnel, without departing from the inventive concept of the premise, various modifications and improvements can be made.These belong to the present invention
Protection domain.
Micropterus salmoides autumn mating system, described method includes following steps：
1) Juvenile stage
Spring bosom ovum, 4~5 two ages big sea bass of old mouth for producing ovum per month, it is more than specification 0.55kg/ tails, physical strong, body colour
It is good, without lopsided, not damaged, disease-free, 1230kg altogether.
2) cement pit is cultivated
Cement pit specification is long 4m × wide 4m × depth 1m, and culvert system, aeration system, refrigeration system are perfect.Cement pit
It cultivates water source and uses lake water or well water.Lake water uses after filter device precipitates mud, filtering organic matter；Well water is by abundant
It is used after sudden and violent gas, oxidation, precipitation.Parent was put into cement pit, breeding density 10.5kg/ ㎡ June 30.Parent just enters cement pit
When, to mitigate parent's stress stimulation, cement pit water temperature is consistent with transport water temperature.Parent was tided over after the laundering period, except disease
Water is not changed during prevention and during cooling, cement pit replaces water body 1/5th daily, and daily soil pick-up removes residual bait excrement.Disease
Evil aim at prevention, high efficiency composition iodine of continuously splashing when parent has just entered pond sterilization it is secondary, backsight parent's situation disinfection and deinsectization.Water
Mud sump water water quality dissolved oxygen is 8~10mg/L, and pH value is 7~8.Death 130kg during parent culture.
Wherein, the laundering period is two weeks.
3) low temperature induction
Parent tides over the laundering period, rejuvenates, and after normally ingesting, in August, 4 start low temperature induction.Induce initial temperature
For 28 DEG C, with 7 days for Unit 1, the 1st unit cooling extent is 3.8 DEG C, is down to 24.2 DEG C；2nd 3.7 DEG C of unit cooling extent,
It is down to 20.5 DEG C；Unit the 3rd keeps 20~21 DEG C of water temperature；4th 3 DEG C of unit cooling extent, is down to 17 DEG C；The cooling of Unit the 5th 3
DEG C, it is down to 14 DEG C；Unit the 6th keeps 14~15 DEG C of water temperature；Unit the 7th heats up 2~3 DEG C, rises to 17 DEG C；Unit 8 heating 3
DEG C, 20 DEG C are risen to, it is consistent with outdoor water temperature；Keep consistent with outdoor water temperature afterwards, water temperature is 18~20 DEG C.
4) nutrition regulation
The cement pit nurturing period feeds bait as the special compound feed of Micropterus salmoides, the morning 8:00th, afternoon 4:00 throws
2 are fed, feeding volume is the 0.5~1% of weight.The additives such as VC, VE, Bei Ruikang, calcium gluconate are mixed in feed, meet sexual gland
Development nutrition element demand raises parent's function and improves Abwehrkraft des Koepers.
5) sexual gland conditioning agent is injected
Parent injected sexual gland conditioning agent after October 7 by temperature-induced, and sexual gland conditioning agent species is Malaysia dioxone
(DOM), luteinizing hormone-releasing hormone analog (LRH-A2), dosage is 1~1.5mg/kg of DOM, LRH-A210~15 μ
G/kg, takes intramuscular injection, and injection position portion is dorsal fin base portion, frequency injection 1 time.
6) parent's reinforced cultivating
October 7, parent was put into 2 plastic, thermal-insulation canopies after injecting sexual gland conditioning agent, equal 5 mu, depth of water 1.2m of area,
Breeding density is 110kg/ mus, is put into parent 1100kg.Change during reinforced cultivating and feed chilled fish, adjust fish body nutrient balance,
Special dietary source is supplemented, promotes sexual gland Rapid development.
7) autumn breeds
Parent pond arrangement breeding fish nest, was spaced 1 meter and placed 1, oviposition on October 27, November 1, batch produced October 26
Ovum.Breeding fish nest is brought into the hatching of hatching workshop, hatched the 1st batch of 2,050,000 tail of autumn Micropterus salmoides spray on November 9.By
November 10 collected Micropterus salmoides fertilized eggs 12,840,000, produced 11,250,000 tail of spray.
(anti-) the breeding emergence statistical form of Micropterus salmoides autumn in 2017
Preferably, the fish be Micropterus salmoides, two ages old mouth.
Wherein, the old mouth is the sea bass next year listed, and two ages were two week old.
The special compound feed of Micropterus salmoides is sold by the joyful the emperor's kindness aquatic feeds Co., Ltd in Zhejiang.
Preferably, in step 3), parent tides over the laundering period, rejuvenates, and after normally ingesting, is lured in August start temperature on the 4th
It leads.It is 28 DEG C to induce initial temperature, and with 7 days for Unit 1, the 1st unit cooling extent is 3.8 DEG C, is down to 24.2 DEG C；Unit the 2nd
3.7 DEG C of cooling extent, is down to 20.5 DEG C；Unit the 3rd keeps 20~21 DEG C of water temperature；4th 3 DEG C of unit cooling extent, is down to 17
℃；Unit the 5th cools down 3 DEG C, is down to 14 DEG C；Unit the 6th keeps 14~15 DEG C of water temperature；Unit the 7th heats up 2~3 DEG C, rises to 17
℃；Unit 8 heats up 3 DEG C, rises to 20 DEG C, consistent with outdoor water temperature；Keep consistent with outdoor water temperature afterwards, water temperature is 18~20
℃。
Preferably, in the step 4), the cement pit nurturing period feeds bait and is raised for the special cooperation particle of Micropterus salmoides
Material, the morning 8:00th, afternoon 4:00 feeds 2, and feeding volume is 0.5~1wt% of weight.VC, VE, Bei Ruikang, Portugal are mixed in feed
The additives such as grape Calciofon, additive amount are 0.5~1wt% of feedstuff feeding amount.Meet gonad development nutrient demand, raise
Parent's function and raising Abwehrkraft des Koepers.
Preferably, in the step 5), parent injected sexual gland conditioning agent, sexual gland tune by low temperature induction after October 7
Agent species is saved as Malaysia dioxone (DOM), luteinizing hormone-releasing hormone analog (LRH-A2), dosage for DOM 1~
1.5mg/kg、LRH-A210~15 μ g/kg, take intramuscular injection, and injection position portion is dorsal fin base portion, frequency injection 1 time.
Preferably, in the step 6), parent is put into plastic, thermal-insulation canopy after injecting sexual gland conditioning agent, is created October
Make breeding environment.Change during reinforced cultivating and feed chilled fish, adjust fish body nutrient balance, supplement special dietary source promotes sexual gland
Rapid development.
Preferably, in the step 7), parent is laid eggs for the first time on the 27th October, and November 1, batch was laid eggs, and was incubated November 9
Dissolve first batch of 2,050,000 tail of spray.
The breakthrough of Micropterus salmoides autumn breeding key technology provided by the present invention can change current Micropterus salmoides seed
Supply time is single, and the culture-cycle is long, and marketable fish concentrates listed price low, and across year listing is of high cost and high temperature marketing risk is high
Present situation provides technical support for the realization of diversification aquaculture model.It establishes for the first time at home and covers the cultivation of parent's cement pit, low temperature
The Micropterus salmoides autumn breeding key technology such as induction, nutrition regulation, the injection of sexual gland conditioning agent, solves Micropterus salmoides marketable fish height
Warm season market supply required seed supply problem in short supply, it is significant, significant economic benefit and society will be generated
Benefit.
It should be noted that the above embodiments are merely illustrative of the technical solutions of the present invention and it is unrestricted.Although with reference to compared with
The present invention is described in detail in good embodiment, it will be understood by those of ordinary skill in the art that, it can be to the technology of invention
Scheme is modified or replaced equivalently, and without departing from the scope of technical solution of the present invention, should all cover the power in the present invention
In sharp claimed range.
Claims (9)
1. a kind of Micropterus salmoides autumn mating system, which is characterized in that described method includes following steps：
1) Juvenile stage
Spring bosom ovum is drained through the Micropterus salmoides of ovum 4 ~ May, it is more than 0.55 kg/ tails of specification, it is physical strong, body colour is good, without deformity,
It is not damaged, disease-free；
2) cement pit is cultivated
Cement pit sets culvert system, aeration system, refrigeration system, cultivates water source using lake water or well water, will be in step 1)
Parent be put into cement pit, breeding density is 10 ~ 12kg/ ㎡, and cement pit water temperature is consistent with parent's transport water temperature, and parent tides over suitable
Should be after the phase, except not changing water during disease control and during cooling, cement pit replaces water body 1/5th daily, daily soil pick-up,
Residual bait excrement is removed, disease aims at prevention, and parent splashes compound iodine sterilization for continuous two days when just having entered pond, cement pit water water quality
Dissolved oxygen is 8 ~ 10mg/L, and pH value is 7 ~ 8；
3) low temperature induction
Parent tides over the laundering period, rejuvenates, and after normally ingesting, starts low temperature induction in early August, induction initial temperature is 28
DEG C, with 7 days for Unit 1, after being progressively down to 14 DEG C, 20 DEG C are warming up to, it is consistent with outdoor water temperature；It keeps afterwards and outdoor water temperature one
It causes, water temperature is 18 ~ 20 DEG C；
4) nutrition regulation
Bait is fed as the special compound feed of Micropterus salmoides, the morning 8:00th, afternoon 4:00 feeds 2, and feeding volume is weight
0.5 ~ 1wt%；
5) sexual gland conditioning agent is injected
Parent is by low temperature induction after injection sexual gland conditioning agent, and sexual gland conditioning agent is Malaysia dioxone DOM, interstitialcellstimulating hormone (ICSH)
Releasing hormone analog LRH-A2, dosage is DOM 1 ~ 1.5 mg/kg, LRH-A2 10 ~ 15 μ g/kg, take intramuscular injection, note
A portion is penetrated as dorsal fin base portion, frequency injection 1 ~ 2 time；
6) parent's reinforced cultivating
Parent is put into spawning pond outside pond after injecting sexual gland conditioning agent, and breeding density is 100 ~ 110kg/mu；
7) autumn breeds
Parent passes through reinforced cultivating, and gonad development situation is periodically dissected and observed in sexual gland Rapid development, and 10 last ten-days period ovaries and spermary are equal
It reaches maturity, parent pond arrangement fish nest is spaced 1 meter and places 1；Spawning pond selects plastic, thermal-insulation greenhouse or outdoor earth pond, plastics
Heat preservation greenhouse temperature is controlled at 20 ~ 28 DEG C, and 17 ~ 20 DEG C of outdoor ponds water temperature periodically looks into fish nest, breeding fish nest is hatched.
2. Micropterus salmoides autumn mating system according to claim 1, which is characterized in that the Micropterus salmoides is old for two ages
Mouth Micropterus salmoides.
3. Micropterus salmoides autumn mating system according to claim 1, which is characterized in that the step 2) parent exists
Cement pit workshop is cultivated, and cement pit specification is long 4m × wide 4m × depth 1m, and water source uses lake water or well water；Lake water passed through
It is used after filter device precipitation mud, filtering organic matter；Well water uses after abundant sudden and violent gas, oxidation, precipitation.
4. Micropterus salmoides autumn mating system according to claim 1, which is characterized in that low temperature lures in the step 3)
It leads, induction initial temperature is 28 DEG C, and with 7 days for Unit 1, the 1st unit cooling extent is 3.8 DEG C, is down to 24.2 DEG C；Unit the 2nd
3.7 DEG C of cooling extent, is down to 20.5 DEG C；Unit the 3rd keeps 20 ~ 21 DEG C of water temperature；4th 3 DEG C of unit cooling extent, is down to 17 DEG C；
Unit the 5th cools down 3 DEG C, is down to 14 DEG C；Unit the 6th keeps 14 ~ 15 DEG C of water temperature；Unit the 7th heats up 2 ~ 3 DEG C, rises to 17 DEG C；8th
Unit heats up 3 DEG C, rises to 20 DEG C.
5. Micropterus salmoides autumn mating system according to claim 1, which is characterized in that injection time in the step 4)
For early October.
6. Micropterus salmoides autumn mating system according to claim 1, which is characterized in that nutrition tune in the step 6)
It controls, VC, VE, Bei Ruikang, calcium gluconate is mixed in the special compound feed of Micropterus salmoides.
7. Micropterus salmoides autumn mating system according to claim 6, which is characterized in that the VC, VE, Bei Ruikang, Portugal
Grape Calciofon total amount is 0.5 ~ 1wt% of the special compound feed feeding volume of Micropterus salmoides.
8. Micropterus salmoides autumn mating system according to claim 1, which is characterized in that the step 6) parent strengthens training
It educates, parent is put into spawning pond after injection, and being changed to chilled fish by feedstuff feeding feeds, every morning 8:00 1, afternoon 4:00
One.
9. Micropterus salmoides autumn mating system according to claim 1, which is characterized in that in the step 7), October 25
Day ovary and spermary are developed to the fifth phase, have reached maturity, October 26 parent pond arrangement fish nest, oviposition on October 27,11
The batch oviposition of the moon 1, breeding fish nest is hatched, hatched the 1st batch of 2,050,000 tail of autumn Micropterus salmoides spray on November 9, is cut
Micropterus salmoides fertilized eggs 12,840,000 were collected to November 10, produce 11,250,000 tail of spray.
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| CN110352877A (en) * | 2019-08-26 | 2019-10-22 | 南京市水产科学研究所 | A kind of method of the full season artificial propagation of Micropterus salmoides |
| CN112741024A (en) * | 2019-10-31 | 2021-05-04 | 华中农业大学 | Out-of-season micropterus salmoides breeding and large-scale fry breeding method |
| CN113841638A (en) * | 2021-08-24 | 2021-12-28 | 中国水产科学研究院珠江水产研究所 | Virus-free fry breeding method for micropterus salmoides |
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| CN110352877A (en) * | 2019-08-26 | 2019-10-22 | 南京市水产科学研究所 | A kind of method of the full season artificial propagation of Micropterus salmoides |
| CN112741024A (en) * | 2019-10-31 | 2021-05-04 | 华中农业大学 | Out-of-season micropterus salmoides breeding and large-scale fry breeding method |
| CN113841638A (en) * | 2021-08-24 | 2021-12-28 | 中国水产科学研究院珠江水产研究所 | Virus-free fry breeding method for micropterus salmoides |
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Address after: 215000 No.88, Yangsha line, chedu village, Yangchenghu Town, Xiangcheng District, Suzhou City, Jiangsu Province Patentee after: Jinchengfu Biotechnology (Suzhou) Co.,Ltd. Address before: 215000 No.88, Yangsha line, chedu village, Yangchenghu Town, Xiangcheng District, Suzhou City, Jiangsu Province Patentee before: SUZHOU JINCHENGFU FISHERY TECHNOLOGY Co.,Ltd. |