CN102100190B - Method for cultivating epinephelus septemfasciatus offspring seeds - Google Patents
Method for cultivating epinephelus septemfasciatus offspring seeds Download PDFInfo
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Abstract
The invention discloses a method for cultivating epinephelus septemfasciatus offspring seeds, which comprises the steps of: culturing the offspring seeds under the conditions that the salinity is 28+/-2, the water temperature is 19+/-2 DEG C, the pH value is 8+/-0.3 and the dissolved oxygen is more than 6mg/l, controlling the seedling distributing density to be 8000-15000/M<3>; before newly hatched larvae enter a pool, putting a composite bacterium (photosynthetic bacterium and nitrobacterium) according to the concentration of 2ppm, adding 0.5ppm of composite bacterium and 1ppm of shrimp feed every day after 5 days, adding 1ppm of composite bacterium and 1ppm of shrimp feed every day after 25 days, and stopping adding the composite bacterium and the shrimp feed after 50 days; illuminating the newly hatched larvae at 2000-4000lx until the newly hatched larvae grow to the 10th day, illuminating the epinephelus septemfasciatus growing from the 11th day to the 30th day at 1000-3000lx and illuminating the epinephelus septemfasciatus growing from the 31st day to the 60th day at 500-1000lx; after the newly hatched larvae enter the pool, sucking and removing pollutants at the bottom of the pool at the beginning of the 30th day; feeding the newly hatched larvae growing from the 4th day to the 7th day SS (Som Somatostatin) type wheel animalcule and hardly developing before the wheel animalcule is fed to the larvae, wherein larva feed comprises the SS small wheel animalcule and common wheel animalcule; and feeding the common wheel animalcule in the newly hatched larvae growing from the 6th day to the 20th day, and developing newly hatched larvae through chlorella pyrenoidosa. The invention has the advantages of reasonable operation process and stable result, and is suitable for culturing cold temperate epinephelus septemfasciatus seeds.
Description
Technical field:
The invention belongs to seawater fish seed rearing technology, specifically the breeding method of seven band grouper seeds in a kind of cold temperate ablen.
Background technology;
Seven band groupers are the famous and precious fingerlings of unique a kind of cold warm nature that are distributed in northern China in the ablen, have the fine and smooth especially deliciousness of taste, be of high nutritive value, be received by the market, seven band groupers are one of ablens that minority can be low temperature resistant in the ablen, and growth is fast, the bodily form is big, disease is few, has wide breed prospect.
Though seven band groupers are high-quality mariculture fingerling, because special ecological physiology characteristic, this kind still has bottleneck at aspects such as parent culture, insemination hatching and seed rearings.The report that does not also have seven band spot fry kinds to cultivate at present both at home and abroad.In other ablens in equal the belonging to together, though the successful leading case of seed rearing is arranged, but all belong to the torrid zone or subtropics kind, as epinephelus akaara (flood ten thousand trees of Xiamen University were published in " Marine Sciences " the 4th phase p:5-8 in 1994), epinephelus fuscoguttatus (tropic sea precious Huang of culturing Co., Ltd in Maoming City Jinyang, Guangdong Province advances light and was published in " aquatic science and technology " the 3rd phase p:14-16 in 2007), all be to grow seedlings water temperature greater than 25 ℃, it is all inequality to use for reference aspects such as illumination regulation and control in the few especially seed rearing of part, water quality control, forage compounding, fortification.
Summary of the invention:
The objective of the invention is that physiological ecological aspect at cold temperate grouper kind is different from other torrid zones or the subtropics ablen provides a kind of simple, the stable cold temperate that adapts with it seven band grouper offspring seed cultivation methods.
The present invention realizes by following technical scheme: its breeding method step comprises: 1), the cloth seedling, 2), regulation and control water quality, 3), illumination, 4), change water, 5), throw something and feed 6) soil pick-up.
1) cloth seedling: the newly hatched larvae cloth seedling density control of acquisition is at 10000-15000 tail/cube water body; Cultivate pool inner water and control at 0.6m deeply, condition of water quality is controlled in salinity 30 ± 2 ‰, 19 ± 2 ℃ of water temperatures, dissolved oxygen in water are 0.5/M greater than 6ppm, little inflation, pneumatic head density
2
2) regulation and control water quality: before newly hatched larvae is put into, add earlier the compound bacteria of 1-2ppm concentration in the pond, behind the 5-25d, add the compound bacteria of 0.5-1ppm and the prawn slice feed of 0.5-1ppm every day, add the compound bacteria of 0.5-1ppm and the prawn slice feed of 0.5-1ppm behind the 25-50d every day, stop using behind the 50d.
Compound bacteria is that photosynthetic bacteria mixes nitrifier, and its ratio is 1: 1.
3) illumination: require 2000-4000lx from the illumination of newly hatched larvae to 10 age in days, 11 age in days to 30 age in days illumination require 1000-3000lx, and 31 age in days to 60 age in days illumination require 500-1000lx.
4) change water: 5d does not add seawater before growing seedlings, the 6-14d that grows seedlings adds clean sea water 5% every day, the 15-20d that grows seedlings changes seawater 10% every day, the 21-30d that grows seedlings changes seawater 30% every day, the 31-50d that grows seedlings changes seawater 800% every day, grow seedlings and day every day change seawater 200% behind the 51d, adopt pipeline mode.
5) throw something and feed:
A, prelarva 4 ages in days to the prelarva 7 ages in days little wheel animalcule of SS type of throwing something and feeding, the wheel animalcule individual lengths, is not strengthened before wheel animalcule is thrown something and fed less than 60 microns less than 120 microns, width;
B, prelarva 6 ages in days to the prelarva 20 ages in days common wheel animalcule of throwing something and feeding, wheel animalcule length are not more than 220 microns, width and are not more than 120 microns, strengthen through chlorella;
C, 18 age in days to the 25 ages in days fairy shrimp nauplius of throwing something and feeding;
D, 20 age in days to the 60 ages in days copepoda of throwing something and feeding;
E, 40 ages in days finish to throw something and feed the micro-capsule mixed bait to growing seedlings
F, 50 ages in days finish to throw something and feed the fresh fish meat gruel to growing seedlings.
6), newly hatched larvae is gone into behind the pond 30d and is risen and absorb bottom, pond dirt every day.
Difference and the beneficial effect of the present invention and other seawater fish offspring seed cultivation methods are as follows:
1, through experiment showed, repeatedly according to the sensory genesis and development of larvae juvenile fish, regulate the intensity of illumination of cultivating in the container, ingest bait and hide the illumination needs of later stage when eating mutually to satisfy.
2, the present invention adopts to throw in the pond and can improve the compound bacteria of water quality, and adjusts certain concentration, all the time the ammonia nitrogen in the water and the control of nitrous acid nitrogen in very low level, guaranteed the latent fixed of water quality; The prawn slice of throwing something and feeding are to have remedied wheel animalcule oligotrophy, have also played the effect of regulating water quality.
3, when the firm opening of newly hatched larvae, the wheel animalcule of throwing something and feeding is not strengthened, and can guarantee that the individual size of wheel animalcule can be swallowed by prelarva, remedies oligotrophy by the prawn slice in the water body, along with the schistostoma of growing of prelarva increases wheel animalcule after the hot housing of throwing something and feeding again.
4, the present invention regulates the intensity of illumination of cultivating in the container by at different development stage, reasonably uses compound bacteria to improve water quality, and according to the development condition of different times and the nutritional need corresponding bait of throwing something and feeding, thereby the incubation rate that reaches healthy seedling is 20%.
Embodiment:
Principle of the present invention is: on the basis of natural imitation ecotope, some physical and chemical factors are made suitable modification more to adapt to growing of larvae juvenile fish, the method for intensive cultivation seven band grouper newly hatched larvae seeds.
Below in conjunction with example in detail technology contents of the present invention is described in detail:
Its breeding method step comprises: 1), the cloth seedling, 2), regulation and control water quality, 3), illumination, 4), change water, 5), throw something and feed 6) soil pick-up.
Adopt this experimental technique that seven band grouper newly hatched larvaes are put into 24 squares cement pit, putting seedling density is 1.0 ten thousand tails/m
3The dark 0.6m of filtering sea in the pond, condition of water quality control in salinity 30 ± 2 ‰, 19 ± 2 ℃ of water temperatures, surface layer of water illumination 4000lx, dissolved oxygen in water be 0.5/M2 greater than 6ppm, little inflation, pneumatic head density; Add the compound bacteria of 2ppm concentration before newly hatched larvae is put into earlier to the pond, 5-25d adds the compound bacteria of 0.5ppm and the prawn slice feed of 1ppm every day, and 25-50d adds the compound bacteria of 1ppm and the prawn slice feed of 1ppm every day, stops using behind the 50d.
Newly hatched larvae rises to 10 age in days illumination requirement 2000-4000lx, 11 age in days to 30 age in days illumination requirement 1000-3000lx, 31 age in days to 60 age in days illumination requirement 500-1000lx.
5d does not add seawater before growing seedlings, and the 6-14d that grows seedlings adds clean sea water 5% every day, and the 15-20d that grows seedlings changes seawater 10% every day, the 21-30d that grows seedlings changes seawater 30% every day, the 31-50d that grows seedlings changes seawater 800% every day, and day every day is changed seawater 200% behind the 51d that grows seedlings, and adopts pipeline mode.
Larvae juvenile fish bait series: SS type wheel animalcule-common wheel animalcule-artemia nauplii-copepoda-micro-capsule mixed bait-self-control fish meat emulsion.Prelarva 4 ages in days to the prelarva 7 ages in days SS type wheel animalcule of throwing something and feeding, 20/ml of density; The common wheel animalcule that prelarva 6 ages in days to prelarva 20 ages in days are thrown something and fed and strengthened through chlorella, 15/ml of density; 18 age in days to the 25 ages in days fairy shrimp nauplius of throwing something and feeding, 5/ml of density; 20 age in days to the 60 ages in days copepoda of throwing something and feeding, 2/ml of density; 40 ages in days finish to throw something and feed the micro-capsule mixed bait to growing seedlings, and the micro-capsule mixed bait is pressed 2h/ time, and joining bait density in the control water when throwing something and feeding is 6/ml; 50 ages in days finish to throw something and feed the fresh fish meat gruel to growing seedlings, and domestication makes the young juvenile fish of seven band groupers concentrate grazing rate to reach more than 80% gradually, and the self-control fish meat emulsion is thrown something and fed 4-6 time every day, and back 1 hour not surplus residual bait is as the criterion to throw something and feed.
Cultivated through 60 days, bring out 48000 tails, seven band grouper juvenile fish altogether, the long 2.5cm of average body, incubation rate 20%.
The fertilized egg of seven band groupers belongs to transparent, buoyant egg.Hatching back body colour is transparent, flies at water surface.Because organ and functional development imperfections such as its eye, fin, body surface pigment, stomach and intestine, its discovery for living body bait, pounce on ability such as catch, ingest a little less than.We adopt than high light 2000-4000lx illumination is convenient to prelarva discovery food, pounces on and catch food.Simultaneously, adopt the ss wheel animalcule than small dimension to meet the swimming of its bore, stomach and intestine and seven band grouper prelarvas and pounce on the food ability; Behind 11 ages in days to fry eye fin, body muscle and the stomach and intestine of 30 ages in days all relatively prosperity get up, find food and pounce on the food ability to increase severely, not as good as prelarva, 1000-3000lx can satisfy its discovery and pounce on the ability of food food for the demand of illumination.Simultaneously, because the enhancing of eyesight possesses the wheel animalcule (common wheel animalcule) of finding and pouncing on the big specification of the ability of catching preferably.And influences such as the shadow that takes place in the management operations for the high light in the external world, administrative staff, sound beginning responsive, frightened, frighten, run away, collide; By the time fry rearing is during to 31 age in days to 60 ages in days, the fry health is more healthy and stronger, and eyesight prosperity, swimming are very capable, except pouncing on the food living bait, also can pounce on anthropophagy worker's mixed bait, the bright material of ice.At this moment, fry has possessed and seeks dark zone, has the environment that covers the place, and is more responsive for the interference in the external world, light, the sound etc., likes dark environment.Accordingly, we adjust to the faint light photograph of 500-1000lx with seed rearing illumination, make it both can obtain (discovery) bait when throwing something and feeding bait, do not make it in the environmental condition of coercing than causing under the intense light irradiation, fear, escape etc. influence its normal life again.
Claims (4)
1. the breeding method of a band grouper seed is characterized in that it being to adopt following incubation step: 1), the cloth seedling, 2), regulation and control water quality, 3), illumination, 4), change water, 5), throw something and feed 6) soil pick-up;
1), cloth seedling: the newly hatched larvae of acquisition is put into and is cultivated the pond, the control of cloth seedling density is at 10000-15000 tail/cube water body, cultivate the control of pool inner water position at 0.5-0.6m, condition of water quality control in salinity 30 ± 2 ‰, 19 ± 2 ℃ of water temperatures, dissolved oxygen in water be 0.5/M greater than 6ppm, little inflation, pneumatic head density
2
2), regulation and control water quality: before newly hatched larvae is put into, the compound bacteria that adds earlier 1-2ppm concentration in the pond, behind the 5-25d, add the compound bacteria of 0.5-1ppm and the prawn slice feed of 0.5-1ppm every day, add the compound bacteria of 0.5-1ppm and the prawn slice feed of 0.5-1ppm behind the 25-50d every day, stop using behind the 50d, described compound bacteria is photosynthetic bacteria and nitrifying bacteria;
3), illumination: newly hatched larvae rises to 10 age in days illumination requirement 2000-40001x, and 11 age in days to 30 age in days illumination require 1000-30001x, and 31 age in days to 60 age in days illumination require 500-10001x;
4), change water: 5d does not add seawater before growing seedlings, the 6-14d that grows seedlings adds clean sea water 5% every day, the 15-20d that grows seedlings changes seawater 10% every day, the 21-30d that grows seedlings changes seawater 30% every day, the 31-50d that grows seedlings changes seawater 50-100% every day, grow seedlings and day every day change seawater 200% behind the 51d, and adopt the flowing water type of rearing;
5), throw something and feed:
A, prelarva 4 ages in days to the prelarva 7 ages in days little wheel animalcule of SS type of throwing something and feeding, wheel animalcule individual lengths less than 120 microns, width less than 60 microns;
B, prelarva 6 ages in days to the prelarva 20 ages in days common wheel animalcule of throwing something and feeding, wheel animalcule length are not more than 220 microns, width and are not more than 120 microns;
C, 18 age in days to the 25 ages in days fairy shrimp nauplius of throwing something and feeding;
D, 20 age in days to the 60 ages in days copepoda of throwing something and feeding;
E, 40 ages in days finish to throw something and feed the micro-capsule mixed bait to growing seedlings;
F, 50 ages in days finish to throw something and feed the fresh fish meat gruel to growing seedlings;
6), soil pick-up; Newly hatched larvae is gone into behind the pond 30d and is risen and absorb bottom, pond dirt every day.
2. seven band grouper offspring seed cultivation methods according to claim 1, when it is characterized in that regulating and control water quality in the compound bacteria ratio of photosynthetic bacteria and nitrifying bacteria be 1: 1.
3. seven band grouper offspring seed cultivation methods according to claim 1, when 4 age in days to 7 ages in days are thrown something and fed SS type steamboat worm when it is characterized in that throwing something and feeding without reinforcement.
4. seven band grouper offspring seed cultivation methods according to claim 1 is characterized in that 6 age in days to the 20 ages in days common wheel animalcule of throwing something and feeding, and strengthen through chlorella.
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| CN102657127A (en) * | 2012-05-28 | 2012-09-12 | 海南省水产研究所 | Facility fry-rearing method for grouper in large water body of outdoor cement pool |
| CN102972649B (en) * | 2012-11-30 | 2014-08-27 | 烟台百佳水产有限公司 | Bait formula of parent fish of epinephelus septemfasciatus |
| CN105660466B (en) * | 2016-01-15 | 2018-04-17 | 中山大学 | A kind of method for controlling illumination to cultivate grouper fry |
| CN108770737B (en) * | 2018-05-23 | 2020-10-27 | 海南晨海水产有限公司 | High-survival-rate and high-growth-rate tiger dragon hybrid spot pond seedling culture method |
| CN111183928B (en) * | 2020-01-20 | 2022-03-18 | 海南晨海水产有限公司 | Indoor cement pond artificial breeding method for oplegnathus punctatus |
| CN111183932B (en) * | 2020-02-26 | 2022-03-22 | 海南晨海水产有限公司 | Breeding method for improving disease resistance and stress resistance of grouper fry |
| CN111194707B (en) * | 2020-02-27 | 2022-03-22 | 海南晨海水产有限公司 | Efficient outdoor ecological pond artificial breeding method for red sea perch |
| CN113854205A (en) * | 2021-11-09 | 2021-12-31 | 宁德市鼎诚水产有限公司 | Large-scale fingerling ecological polyculture method for epinephelus akaara |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1653892A (en) * | 2004-11-22 | 2005-08-17 | 珠海市南桦水产科技有限公司 | Aquatic breeding method for Epinephelus coioides |
| CN101375673A (en) * | 2007-08-27 | 2009-03-04 | 海南大学 | Industrialized artificial seedling cultivation method for rockfishes |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1653892A (en) * | 2004-11-22 | 2005-08-17 | 珠海市南桦水产科技有限公司 | Aquatic breeding method for Epinephelus coioides |
| CN101375673A (en) * | 2007-08-27 | 2009-03-04 | 海南大学 | Industrialized artificial seedling cultivation method for rockfishes |
Non-Patent Citations (1)
| Title |
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| 刘新富等.七带石斑鱼人工繁育技术研究进展.《中国水产科学》.2010,第17卷(第5期),1128-1135. * |
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Application publication date: 20110622 Assignee: FANGCHENGGANG HAISHITONG SEAFOOD CO.,LTD. Assignor: YELLOW SEA FISHERIES Research Institute CHINESE ACADEMY OF FISHERY SCIENCES Contract record no.: 2015450000001 Denomination of invention: Method for cultivating epinephelus septemfasciatus offspring seeds Granted publication date: 20130717 License type: Common License Record date: 20150108 |
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