A kind of method of the red clam worm of indoor circulating water three-dimensional culture
Technical field:
The present invention relates to aquaculture, specifically a kind of method of red clam worm of indoor circulating water three-dimensional culture.
Technical background:
Red clam worm formal name used at school Perinereis nuntia, local name " rock worm ", belong to Annelida, polychaeta, Nereidae a kind of end dwell without vertebra marine organisms. Usually inhabit in Intertidal zone rocky coast coarse sand or Corallium Japonicum Kishinouye layer of sand, in the Concha Ostreae in Intertidal zone rocky coast Zhong Qu and lower district and Sargassum clump, in subtidal zone Alga Sgrgassi Enerves and Zostera marina clump. In nursery, feedstuff, additive, it is the high-quality live body natural bait of fish, shrimp, Eriocheir sinensis artificial breeding and cultivation, it is as prawn bait, not only parent's shrimp addiction, and can prevent gonad from degenerating, the content of its Main Ingredients and Appearance " eicosapentaenoic acid " is far above brine shrimp eggs, and its comprehensive nutrition, attractant are strong, utilization rate is high, can improve shrimp shoot survival percent and resistance against diseases; Clam worm can be cooked the feed additive and high protein that non-nutritive phagostimulant is green, environmental protection after drying. In nutritional health food, clam worm has effect of relaxing muscles and tendons to promote blood circulation, temperature spleen invigorating helmet, nourishing YIN void, antiasthmatic-antitussive, anti-cancer, anticancer, enhancing immunity and radioprotective, preventing thrombosis disease, preventing arteriosclerosis, defying age, raising memory;Rich in protein content up to 68%, amino acid contained quite complete, include required by people, animal, fowl, fish whole must aminoacid; After clam worm is dried, also known as " dragon intestinal do ", for the precious famous dish of senior feast, people from Pingtan using the bundled clam worm of bunchy in the valuable gift as present close relatives and good friends; Fujian, Guangdong, ALONG GUANGXI COAST resident are also nutrition treasure depending on the clam worm that gonad is ripe, after drying, boil Tang Bai such as milk, and taste is extremely delicious, and concentration is big, has the title of " natural monosodium glutamate ", and loose delicious and crisp after fried, for delicacies of going with wine. In medicine, aminoacid, trace element and the vitamin, wherein zinc 290ppm wanted containing a large amount of needed by human body in clam worm body, iodine 100.6ppm, selenium 1.13ppm, vitamin E2 6.70ppm, aminoacid 540mg/g, fibrinolysin 1000-2000 unit, it is especially enriched in fibrinoclase, three kinds of enzyme systems such as activator of plasminogen, collagenase, are effective health foods of prophylaxis of hypertension, arteriosclerosis and allaying tiredness; Nereid kinase contained in clam worm body has the function of the treatment thrombotic disease such as cerebral thrombosis, myocardial infarction; Contained substantial amounts of unsaturated fatty acid has enhancing immunity, improves effect of memory, also has antithrombotic, prevents the effect of arteriosclerosis; In addition contain abundant hydroxyamino, there is the effect of skin maintenance defying age. At present with clam worm be raw material production seafood health food and the marine organisms medicine technology of anticancer, anti-cancer and radioprotective, obtained national patent. In insecticide, clam worm is a kind of marine drug inherently, has been used for extracting nereistoxin. Padan's (cartap) be in human history first time successfully utilize zootoxin to carry out the animal sources insecticide of bio-mimetic syntheses, be wide spectrum, efficiently, the novel pesticide of low toxicity, low-residual. In bait, it is soft that fresh and alive red clam worm has meat, and toughness is strong, resistance to stings, and under the effect of illumination, body surface can send fluorescence, and color and luster is eye-catching, the advantage that fishy smell is dense, is the superfine product in nereidiformia bait. Various reasons causes clam worm, and in international market, supply falls short of demand, is called " the red clam worm " of " gold clam worm " by Japanese, and price is up to per kilogram 10,000 yen.
Present natural clam worm is fewer and feweri, and the demand of clam worm increases year by year. Owing to nutritive value and the commercial value of clam worm are very high, it has also become a current popular breed variety. The breeding way of current clam worm mainly has:BeachExtensive aquaculture, the semi-artificial seedling cultivation of beach, extensive stylePondCultivation, although these breeding way cost of investment relative moderate, but owing to outdoor clam worm natural enemy is a lot, determine that yield will not be too high, during results product, cost of labor is too high, and inside even from weather is very big, can only seasonal produce. The idle facilities utilizing fish shrimp crab Bei Yumiaochang carries out batch productionArtificial cultivation, first artificial breeding in the cement pit not spreading sand, when germling adheres to soon, to throw sand start cultivation, half a year is up to selling specification, although reduced investment, but the relatively low ability 3kg/m of yield2Left and right, Factory Building utilization rate is not high, affects yield, and energy waste is serious, increases aquaculture cost.
Now with various experts and scholars, clam worm is studied, it was also proposed that various more advanced cultural methods. Such as " aquaculture " second phase in 2010, Huang Jinguang, woods wealth " red clam worm cement pit intensive high yielding culture technology " utilize existing prawn seed-rearing field cement pit and oxygen supplement inflation, add control temp, enter draining, the installations and facilities such as sea water sedimentation and filtration ditch. Parent worm cultivates throw something and feed every day chrysophyceae, little diatom, ocean rhodotorula and self-control mixed feed 3~4 times, and throw something and feed day single-cell algae 150,000~200,000 cells/L and self-control cooperation 30~50g/m2, control water temperature 23~25 DEG C, salinity 28~30.And adopt contracting light technology to promote parent gonad maturity. Adult cultivation phase casting bed thickness is 12~15cm, and particle diameter is the sandstone of 0.5~1.0cm, and breeding density is 8000~12000/m2, temperature of cultivation is 6~30 DEG C, and salinity is 20~30. Timing simulation fluctuation every day tide 3 times, water logging stops 30min after there be not casting bed and discharges, during water inlet, clam worm will get out casting bed and out looks for food, just bait throwing in can be carried out, bait is 70% self-control powder+30% diatom powder and chrysophyceae powder, bait throwing in requires that uniformly daily ration of feeding is the 3%~5% of clam worm body weight, takes the circumstances into consideration increase and decrease according to the residual bait amount in casting bed surface. To rinse casting bed according to situation at the bottom of casting bed pond and suitably to discharge some improver of water quality during cultivating, keep breeding environment cleaning, improve cultivation survival rate. From the cultivation through 5~6 months of 2~3cm larva, commercial specification 10~12cm adult, its cultured output 4~5kg/m can be reached2。
Huang Jinguang, Lee's work, adopted " clam worm batch production is without sandy soil ecology high yielding culture technical research " of expressing gratitude for a favour utilize thin base river hedge, river hedge, ulva, Anhydrite Herba braseniaeel., Entermorpha, thorn-like fish to dwell the tangleweeds such as tongue as cultivating adherance, carrying out clam worm batch production without sandy soil ecologic breeding, yield reaches 15~20,000 grams per square meter. With cement pits such as fish and shrimp seed rearing pond, abalone culture ponds. Water temperature 15~33 DEG C, salinity 15~32, illumination 5000~15000 lux, more than dissolved oxygen amount 4 mg/litre. Seed requires health, not damaged, lovely luster, body surface gloss, body length 20~50 millimeters, and specification tool 20~35 just saves, and breeding density is 50,000~10,000/square meter, along with clam worm individuality increases, it is necessary to constantly dredges and supports, but cultivation density is not less than 10,000 tails/square meter. Absorb breeding water body nutritive salt owing to tangleweed has and increase the effect of dissolved oxygen. Therefore, without often changing water in nereis culture process, need only adding appropriate unicellular algae solution every day, and within 15~30 days, change water 1 time according to water quality situation in pond in pond, each quantity of exchanged water is 1/3~2/3. Bait feeding: bait throwing in every day 1~2 time, bait is based on flat algae, Dicrateria inornata, pavlova viridis, Entermorpha, fish flour and eel compound feed etc. Various unicellular algae solutions concentration of throwing something and feeding is 100,000~200,000 cells/ml; The feeding volume of Thallus Porphyrae powder, fish flour and eel compound feed is the 1%~5% of clam worm body weight, and visual clam worm situation of ingesting is grasped flexibly; Entermorpha is the adherance that clam worm is good, is again the bait of high-quality, should often supplement as required. Inflation: high density nereis culture should keep paying no attention to continuously disconnected inflation, especially night and high temperature season and overcast and rainy, will suitably strengthen aeration quantity, keep more than water body dissolved oxygen 4 mg/litre. Tangleweed is utilized to cultivate adherance as clam worm, the two complements each other in ecological functions, constitutes the cultivating system of a kind of combined type: wherein because of bait input, silkworm body metabolic exhaustion water body dissolved oxygen in clam worm system, reduce pH, release inorganic nutrient salt, causes loads of nutrition; Tangleweed then carries out photosynthesis, absorbs inorganic nutrient salt, produces oxygen, improves water body pH, thus the nutritive salt reducing breeding water body pollutes, and improves water quality, is conducive to stablizing of breeding water body environment, improves the yield of nereis culture. It addition, the author also finds in nereis culture process, tangleweed is not only the boarding ground that clam worm is good, tongue etc. or the bait of clam worm high-quality and some kind such as Entermorpha therein, Anhydrite Herba braseniaeel., thorn-like fish dwell.After clam worm ingests, silkworm body looks healthy and strong especially, loose, and survival rate is up to more than 98%. Batch production, without sandy soil ecologic breeding clam worm, is use ecological principle, regulates and controls cultivating system by human intervention, promotes the material benign cycle of silkworm algae complex culture system and energy Flow to reach best growth conditions. Clam worm utilizes tangleweed as perching hidden place and food nutrition source, and simultaneously the Excreta of clam worm and residual bait are thin base river hedge, river hedge, ulva etc. provides nutrient substance. Tangleweed absorbs water body inorganic nutrient salt, and the self-purification capacity of system strengthens, and improves the water quality of culturing pool, substrate, and the carrying capacity of breeding water body is greatly improved, and can obtain the efficient effect of stable high yield.
" Shandong fishery " the 22nd volume second phase in 2005, " the industrialized culture technology of red clam worm " bait of Qu Hongxia, Meng Wei is best with the growth result of Sargassum (Entermorpha), can improve the survival rate of clam worm, and silkworm body also looks healthy and strong, loose.
Du Rongbin, Wang Aimin, the condition of culture of Perinereis nuntia parent has been studied by Yushan Hill blue or green (" Marine Sciences " the 9th phase in 2009) " cultivation of Perinereis nuntia parent ", and result shows: the suitable cultivation temperature of Perinereis nuntia is 16~32 DEG C, and salinity is 25~32. Being preferred with powder bait during cultivation, ingest active for 16~32 DEG C, the time of ingesting is 15~20min, and less than 16 DEG C food rations reduce, and substantially do not ingest for less than 12 DEG C. 19~20 DEG C, keep under wet condition, 28d. heteronereis of can surviving is formed relevant with temperature, particularly after experienced by certain hypothermic phase (2~4 DEG C), improve temperature again to 18~25 DEG C, heteronereis can be effectively facilitated formed. in artificial cultivation situation, the formation of heteronereis and group float there is not Behavior rule, as long as there being faint illumination after maturation, the covering all visible heteronereis group of tank that adds water any time floats;
" Chinese Fishery " 08 phase Chen Mao brightness in 2008" Perinereis nuntia industrial artificial cultural technique " adult cultivation phase casting bed thickness 12cm, particle diameter 0.5~1cm, throw in density 6000 tails/m2~10000 tails/m2. Simulating fluctuation tide every day 3 times, water logging drains after keeping 10 minutes after there be not casting bed, bait throwing in every day 3 times (70% self-control powder, 30% diatom powder), daily ration of feeding 30g/m2~50g/m2, take the circumstances into consideration increase and decrease according to the residual bait amount in casting bed surface. Summer, from 3cm larva support to the 10cm adult culture-cycle be 2 months; About 3~5 months culture-cycle in winter, its cultured output 3kg/m2~4kg/m2。
" Chinese Marine University " " when Perinereis nuntia (PerinereisNuntiaSavigny) artificial cultivation research of parents culture ecological condition and reproductive biology " refined for Du Rong in 2004, Perinereis nuntia belongs to the end and dwells kind, routing up the external bait eaten the granular bait for fish sunk to the bottom and float on the water surface by kiss when ingesting, water temperature of ingesting is 16~32 DEG C. Growth body, at 19~20 DEG C of temperature, as long as keeping embedding substrate moist, can be survived about 1 month, having very strong annidation. Test result indicate that, it is possible to adapt to the environment of (the suitableeest) water temperature 0~32 (16~32) DEG C, salinity 5.72~42.91 (16.16~31.69), pH value 7~9. The cultivation of parent main with water temperature, illumination relevant, germinal epithelium (gonad) all can be grown under 16~32 DEG C of water temperature conditions that can ingest, and during sexual maturity, except a head part, each body segment all has sexual cell.It is 18~32 DEG C that sexual maturity forms the water temperature of obform body, and water temperature falls before, after slowly heat up again and cultivate than continuous high water temperature that obform body is formed facilitation is obvious, the formation of obform body is also obviously promoted effect by the prolonging light time. Under manual control condition, group is floating does not have obvious daily rhythm. Being formed after obform body, formalness changes, and health rear portion body segment shortens, and broadens, flat. Rear portion parapodum also morphs, and the base portion of the back of the body of parapodum, abdomen palpus expands, flat in fan-shaped, and in parapodum, blood capillary change is very flourishing, and bristle is also become fan-shaped pulpous state bristle from spiniger. After forming obform body, female and male sex-cell is full of body cavity, and trophocyte disappears, and digestive tract attenuates, amyotrophy, and body segment barrier film disappears. Perinereis nuntia namely can natural propagation can also artificial insemination, all can obtain the rate of fertilization of more than 95%, the female obform body eggs on average amount of artificial cultivation is 13000~18000, ovum diameter 240 μm, demersal egg, tool viscosity. The suitableeest incubation temperature is 18~25 DEG C, salinity is 25~32. At 25 DEG C, can hatching through 65h, incubation rate is up to 92%. Perinereis nuntia ovum is developed to nectocaete through spiral cleavage in film, after hatching, is 3 setiger juveniles, and seeking the end during to 4 setiger juvenile gradually dwells life, now can throw in adherance, and juvenile stage cultivates controlling water temperature 24~28 DEG C, salinity 15~42. 10 have just saved before germling, it is possible to throw something and feed Micro Algae and fine powder bait, the bait of slightly larger particle diameter of should throwing something and feeding thereafter.
Entermorpha, frond grass green, tubulose film quality, to grow thickly, major branch is obvious, and branch is elongated, high up to 1 meter. Base portion is attached on rock with holdfast, is grown in middle tide band beach, on chad. The optimum temperature of Entermorpha growth is 15~25 DEG C, and salinity 25~32 ‰, water flow velocity 10~30cm/s, intensity of illumination is 100~120umol/ (m2.s)。
In sum, Perinereis nuntia belongs to the end and dwells kind, routs up the external bait eaten the granular bait for fish sunk to the bottom and float on the water surface by kiss when ingesting, optimum growth temp is 16~32 DEG C, and salinity is 25~32 ‰, pH value 7~9, illumination 5000~15000 lux, more than dissolved oxygen amount 4 mg/litre. The suitable clam worm of temperature grows quickly, such as summer, from 3cm larva support to the 10cm adult culture-cycle be 2 months, and the culture-cycle in winter takes around 3~5 months. The cultivation of parent main with water temperature, illumination relevant, germinal epithelium (gonad) all can be grown under 16~32 DEG C of water temperature conditions that can ingest, and during sexual maturity, except a head part, each body segment all has sexual cell. Heteronereis is formed relevant with temperature, particularly after experienced by certain hypothermic phase (2~4 DEG C), improve temperature again to 18~25 DEG C, heteronereis can be effectively facilitated formed, cultivating than continuous high water temperature and obform body is formed facilitation substantially, the formation of obform body is also obviously promoted effect by the prolonging light time. In artificial cultivation situation, the formation of heteronereis and group float there is not Behavior rule, as long as there being faint illumination after maturation, the covering all visible heteronereis group of tank that adds water any time floats; Perinereis nuntia both can natural propagation can also artificial insemination, the suitableeest incubation temperature is 18~25 DEG C, salinity is 25~32. At 25 DEG C, can hatching through 65h, incubation rate is up to 92%. Perinereis nuntia ovum is developed to nectocaete through spiral cleavage in film, after hatching, is 3 setiger juveniles, and seeking the end during to 4 setiger juvenile gradually dwells life, now can throw in adherance, and juvenile stage cultivates controlling water temperature 24~28 DEG C, salinity 15~42.10 have just saved before germling, it is possible to throw something and feed Micro Algae and fine powder bait, the bait of slightly larger particle diameter of should throwing something and feeding thereafter. The tangleweeds such as Entermorpha are not only the boarding ground that clam worm is good, but also are the bait of clam worm high-quality, and after clam worm ingests, silkworm body looks healthy and strong especially, loose, and survival rate is high. Clam worm utilizes tangleweed as perching hidden place and food nutrition source, simultaneously the Excreta of clam worm and residual bait are Entermorpha etc. tangleweed provides nutrient substance, tangleweed absorbs water body inorganic nutrient salt, the self-purification capacity of system strengthens, the water quality of culturing pool, substrate, the carrying capacity of breeding water body can be greatly improved, the efficient effect of stable high yield can be obtained.
My a kind of indoor circulating water stereoscopic multipurpose aquatic products ecology rearing device, it includes box for breeding, shelf, biofilter, tank, water circulating pump, aquaculture constant temperature machine, aerator and connection pipeline thereof, it is characterized in that: described box for breeding piles up formation three-dimensional culture structure on shelf, described box for breeding is by casing, the bottom interlayer formed with microwell plate at the bottom of casing, that offers on box body wall enters osculum and gas transmission pore composition, aeration tube is spread at the bottom of described casing, aeration tube is connected with appendix, aeration tube covers microwell plate, the mixture of one layer of medical stone granule: coral sand particulate quartz sand grains is spread above microwell plate, mixture is splashed useful vaccine, water inlet pipe is along tank wall direct-furnish, water-level regulator there is filter cap discharge superfluous water. by cultivation casing etc. is done isothermal holding to save the energy, utilize aquaculture constant temperature machine that one optimum growth temp is set for aquatic products, covering microwell plate formation bottom interlayer on cultivation box bottom paving aeration tube and can pass through micro-pore oxygenation, oxygen supplement uniformly, is not stayed dead angle, is not stimulated aquatic products, the mixture of one layer of medical stone granule: coral sand particulate quartz sand grains useful vaccine of splashing wherein is spread on microwell plate, saturated dissolved oxygen supply is utilized to cultivate probiotics, suppress harmful bacteria, decompose the residual bait of feces, utilize medical stone granule: Adsorption of Heavy Metals to purify water, utilize coral sand granule to regulate pH value. so there is good substrate, it is possible to reduce the generation of aquatic animal disease, laid a solid foundation for producing green non-pollution food, suitable water temperature, saturated dissolved oxygen can improve the density of aquaculture of aquatic animal, improve survival rate and feed conversion rate, promotes aquatic animal growth, reduces the breeding production cycle.
Circulating water cultivation can solve aquaculture water emission pollution problem, is the inexorable trend of following aquaculture industry development; Three-dimensional culture can make the utilization rate of breeding plant reach 130%~160%; Indoor circulating water three-dimensional culture is controlled due to all cultivating conditions, only need to reasonably adjust, so that it may obtain the efficient effect of stable high yield. Search element inquiry by patent, there is presently no the application of clam worm three-dimensional culture aspect.
Summary of the invention:
It is an object of the invention to solve the defect that existing nereis culture mode exists, the growth characteristics according to red clam worm and Entermorpha, in conjunction with the advanced experience about experts and scholars, it is proposed to a kind of method of red clam worm of indoor circulating water three-dimensional culture. By the batch production ecologic breeding of real meaning, the four seasons produce, to realize nereis culture high yield, stable yields, efficient effect.
It is an object of the invention to be reached by following measure, a kind of method of red clam worm of indoor circulating water three-dimensional culture includes preparation and the cultural method of cultivation equipment, it is characterised in that: described cultivation equipment includes a kind of indoor circulating water stereoscopic multipurpose aquatic products ecology rearing device and light compensating lamp; Described ecology rearing device includes box for breeding, shelf, biofilter, tank, water circulating pump, aquaculture constant temperature machine, aerator and connecting line thereof; Described light compensating lamp is arranged on bottom the shelf plywood above box for breeding; At least setting up a sand filter and water tank before described biofilter and tank water inlet pipe, described sand filter and water tank are connected with sea water pump;
Described cultural method includes as follows:
(1) material:
(1) clam worm seed source: artificial collection from numerous and seabeach is buied;
(2) tangleweed Entermorpha source: artificial from numerous and natural waters collection, the holdfast that natural waters gathers is attached on chad;
(3) box for breeding prepares: lay mixture and the chad of one layer of medical stone granule: coral sand particulate quartz sand grains at the bottom of box for breeding;
(4) aquaculture water: with sea water pump from natural waters or underground sea water well extracting seawater to sand filter, through sand filtration to water tank, in water tank, water sterilization is sterilized, after precipitating 24 hours, enter biofilter, enter back into tank, being squeezed into box for breeding by water circulating pump, keep water level 25~26cm, salinity is 25~32 ‰, pH value 7.5~8.6, water flow velocity 10~30cm/s;
(2) method:
(1) seed put in a suitable place to breed before preparation:
1. equipment and the sterilization of the instrument of use: cultivation bottom, tank wall and all medical stone granule :s, coral sand granule, quartzy sand grains, chad, biofilter, tank material therefor are all cleaned and disinfect, after placing 4 hours laid again, inject the sea water that filtration treatment is crossed, water body disinfection is sterilized, ON cycle water pump, allows water circulate sterilization that pipeline is carried out disinfection; After 24 hours, useful vaccine of splashing in filter material for biological filter and box for breeding, open aerator, aquaculture constant temperature machine, temperature is set in 25 ± 2 DEG C, about dissolved oxygen amount 8 mg/litre, minimum cannot be below 5 mg/litre, support water about two days, cultivate probiotics;
2. the cultivation of single-cell algae: carry out single-cell algae cultivation in special box for breeding, the bait of abundant high-quality is prepared for nereis culture, single-cell algae has chrysophyceae, flat algae, pavlova viridis, Nannochloropsis oceanica, close water intaking valve, open light compensating lamp, intensity of illumination is 5000~15000 luxs, keeps water temperature 25 DEG C, it is possible to Fast-propagation;
3. the input of tangleweed Entermorpha: the tangleweed Entermorpha disinfection that will collect from natural waters, then render to equably in box for breeding, water flow velocity is transferred to about 15cm/s, open light compensating lamp, set intensity of illumination as 5000~15000 luxs, accelerate the growth of tangleweed Entermorpha;
(2) seed is put in a suitable place to breed and daily management:
1. the selection of seed with put in a suitable place to breed: seed require health, not damaged, lovely luster, body surface gloss, size is not limit; Size clam worm branch mailbox is put in a suitable place to breed, and breeding density is 50,000 tails~10,000 tails/square meter, along with clam worm individuality increases, it is necessary to constantly dredges and supports, but cultivation density is not less than 10,000 tails/square meter, gathers before the seed buied enters box for breeding from seabeach and wants first disinfection;
2. water is changed: owing to adopting circulating water cultivation, tangleweed Entermorpha is utilized to adhere to culture medium as clam worm, tangleweed Entermorpha has the effect absorbing breeding water body nutritive salt and increase dissolved oxygen, therefore, breeding process need not change water, only need in box for breeding, add appropriate unicellular algae solution every day;
3. bait feeding: bait throwing in every day 1~2 time, bait be Entermorpha powder and small fish, shrimp, shellfish meat powder broken after the mixture of meat paste, day feeding volume be the 1%~5% of clam worm body weight, visual clam worm situation of ingesting is grasped flexibly; Owing to Entermorpha is the good adherance of clam worm, it is again the bait of clam worm high-quality, should often supplement as required;
4. inflation: high density nereis culture should keep inflating uninterruptedly, keeps in water body about dissolved oxygen amount 8 mg/litre, minimum cannot be below 5 mg/litre;
5. light filling: owing to adopting three-dimensional culture, box for breeding light being blocked to some extent, can affect the growth of tangleweed Entermorpha and clam worm, therefore, daytime every day to open light compensating lamp, and intensity of illumination is 5000~15000 luxs;
6. daily management: observe clam worm every day and ingest, grow and streamflow regime; Owing to being stamped filter cap on water-level regulator, it can stop the escape of clam worm, also can be blocked by Entermorpha and clam worm cause emitting excessive, it is necessary to note removing; Conscientiously carry out the growth measurement of clam worm, density measurement, provide foundation for bait throwing in, subsequent production management;
7. results: when clam worm individuality grows to more than 100mm, can gather in the crops when specification reaches within 1000/kilogram, first close water intaking valve, open box for breeding bottom row water valve again, put clean aquaculture water, sort out and shake tangleweed Entermorpha and come off by clam worm, the clam worm adult collected is sorted, the body length box for breeding that returns less than 100mm continues cultivation, long for body more than 100mm specification is reached the commercial insect within 1000/kilogram and carries out packaging sale, the clam worm of the bigger individuality of part is individually chosen as parent for breeding; Clam worm package carrier thing is vermiculite power, and vermiculite power first cools to 18~20 DEG C of moisturizings, fills 5 boxes, every box two-layer, every layer of dress clam worm 1 kilogram for one case, keeps 18~20 DEG C with dry ice homoiothermic in case, send airport air transport outlet or domestic; The commercial insect gathered in the crops lives and sells or drying sale, avoids existing clam worm bait and sells the dull and rush season existed, to obtain higher profit;
(3) parent worm is cultivated and artificial breeding:
1. the big individual clam worm individually picked out is put into hot housing in special box for breeding, close water intaking valve, artificial gradually reduce the water temperature to about 4 DEG C in box for breeding constant temperature one day by force, slowly heating up every day again, until being raised to 23~24 DEG C, then setting constant temperature 23 DEG C, more than 16 DEG C water temperatures are thrown something and fed bait 2~3 times every day, day feeding volume is the 5% of clam worm body weight, bait be Entermorpha powder and small fish, shrimp, shellfish meat powder broken after the mixture of meat paste, the prolonging light time promotes parent gonad maturity;
2., when clam worm parent gonad maturity, build there occurs notable change, and in the middle part of clam worm body, parapodum becomes flat, bristle is elongated, clam worm body substantially defines front portion, middle part, rear portion, and hereafter clam worm body is significantly reduced, and body broadens, 2nd district before and after being formed, proparea parapodum is not deformed, and back zone parapodum is flat, and bristle becomes paddle, so far, the abnormal one-tenth heteronereis of clam worm; In order to avoid inbreeding causes degenerate problem, should select, from different box for breeding, the male and female heteronereis that body colour is bright-coloured, mechanical is hindered, energetic, gonad is full after secondary and match; The discrimination method of male and female heteronereis is: male heteronereis a milky white colour band of longitudinal direction occurs at body center back, female inconspicuous, and male heteronereis anal segment has Flos Chrysanthemi shape projection, female then without this feature; First male and female heteronereis branch mailbox is supported several hours temporarily, putting into by the proportional paired of male and female 7: 1 lays eggs in net cage in special box for breeding again, male and female heteronereis is known from experience quickly wedding dance and lays eggs, arranges essence, wedding dance is laid eggs, is arranged essence and terminate, pull the dead clam worm sinking to bottom out, wash germ cell several times, keep water temperature 25 ± 2 DEG C, tune up aeration quantity and stir the end, in order to avoid germ cell is sunk; Within about 2 days, just can hatching three and just save nectocaete, the larva just hatched is with internal elgen ball for nutrition, it is not necessary to bait throwing in; After 2 days, paedomorphosis has just saved nectocaete to four, and elgen ball disappears, it is necessary to bait throwing in time, bait is based on single-cell algae marine yeast, chrysophyceae, flat algae, bait throwing in every day three times, throw something and feed ocean rhodotorula 0.5~1.0mg/L every time, single-cell algae 100,000~200,000 cells/L;When growing to five firm joint nectocaetes, peritroch fades away, travelling reduced capability, sink to the bottom, starting to transfer to the end dwells life of crawling, and at this moment can be properly added Entermorpha powder of throwing something and feeding, chrysophyceae powder, Spirulina powder 0.5~1.0mg/L, and falls case and divide and proceed to larvae cultivation case and continue to cultivate; Larvae cultivation breeding density is 200,000~300,000 tails/square meter, bait throwing in every day 5 times, bait be 80% Entermorpha powder, chrysophyceae powder, Spirulina powder+20% small fish, shrimp, shellfish meat powder broken after meat paste, daily ration, feeding quantity 3~5 grams per square meter; Growth along with children's clam worm body length, emerging at any time point pool cultivated, children's clam worm body length grows to 2~3cm, and namely 11 just more than joint, breeding density is 50,000~10,000/square meter, bait throwing in every day 1~2 time, bait be Entermorpha powder and small fish, shrimp, shellfish meat powder broken after the mixture of meat paste, day feeding volume be the 1%~5% of clam worm body weight, visual clam worm situation of ingesting is grasped flexibly, owing to Entermorpha is the good adherance of clam worm, it is again the bait of clam worm high-quality, should often supplement as required.
(4) suggestion:
1. the condition required with a lot of aquacultures overlaps, if Eriocheir sinensis water temperature is when 15-26 DEG C, food ration is big, grow the fastest, clam worm optimum growth temp is 16~32 DEG C, salinity is 25~32 ‰, pH value 7.5~8.6, therefore multi items aquatic products can be allowed to hold concurrently support simultaneously, cultivate again clam worm as supported Eriocheir sinensis temporarily simultaneously, the optimum water temperature 24 ± 2 DEG C of they growths it is set in aquaculture constant temperature machine, although being Relationships between Natural Enemies between them, but they are in respective box for breeding, it is independent of each other, clam worm fed by the residual bait of available Eriocheir sinensis again, Eriocheir sinensis is fed with dead clam worm and disability clam worm, both the waste of bait had been reduced, high economic benefit can be obtained again.
2. this method is used for cultivating red clam worm, but is not limited to cultivate red clam worm, it is also possible in the clam worm cultivating other kind.
The invention has the beneficial effects as follows: owing at indoor circulation water termostat three-dimensional culture, the utilization rate of breeding plant 1. can be made to reach 130%~160%. 2. all cultivating conditions are controlled, it is possible to arbitrarily arrange to realize annual four seasons production. 3. interlayer oxygen supply bottom box for breeding, it is ensured that without dead angle, have saturated dissolved oxygen to supply, suitable water temperature adds saturated dissolved oxygen supply, can improve cultivation density, improve survival rate and feed conversion rate, promotes aquatic animal growth, reduces the breeding production cycle. 4. ecologic breeding, it is possible to reduce the generation of disease. 5. omnibearing isothermal holding, it is possible to reduce substantial amounts of energy waste, reduces aquaculture cost. 6. bait adopt Entermorpha powder and small fish, shrimp, shellfish meat powder broken after the mixture of meat paste, meat and vegetables collocation is more nutritious. 7. by the tangleweeds such as the Entermorpha attachment culture medium as clam worm, the tangleweeds such as Entermorpha are not only the boarding ground that clam worm is good, but also be the bait of clam worm high-quality, after clam worm ingests, silkworm body looks healthy and strong especially, loose, survival rate is higher, clam worm utilizes tangleweed as perching hidden place and food nutrition source, simultaneously the Excreta of clam worm and residual bait are Entermorpha etc. tangleweed provides nutrient substance, tangleweed absorbs water body inorganic nutrient salt, the self-purification capacity of system strengthens, the water quality of box for breeding, substrate is improved, the carrying capacity of breeding water body can be greatly improved, obtain stable high yield. 8. pay equally, the costly red clam worm of culturing economic, higher income can be obtained. 9. indoor circulating water three-dimensional culture clam worm is invested relatively big at first glance, but sees the benefit that can obtain several times higher than other breeding way for a long time.10. can hold concurrently foster by multi items aquatic products simultaneously, utilize it complementary, both reduced the waste of bait, high economic benefit can be obtained again. 11. this method can also be used for cultivating the clam worm of other kind.
Detailed description of the invention:
A kind of method of red clam worm of indoor circulating water three-dimensional culture includes preparation and the cultural method of cultivation equipment, it is characterised in that: described cultivation equipment includes a kind of indoor circulating water stereoscopic multipurpose aquatic products ecology rearing device and light compensating lamp; Described ecology rearing device includes box for breeding, shelf, biofilter, tank, water circulating pump, aquaculture constant temperature machine, aerator and connecting line thereof; Described light compensating lamp is arranged on bottom the shelf plywood above box for breeding; At least setting up a sand filter and water tank before described biofilter and tank water inlet pipe, described sand filter and water tank are connected with sea water pump;
Described cultural method includes as follows:
(1) material;
(1) clam worm seed source: artificial collection from numerous and seabeach is buied, and seabeach gathers the size buied and do not limit;
(2) tangleweed such as Entermorpha source: artificial gather from numerous and natural waters, the best holdfast that natural waters gathers is attached on chad;
(3) box for breeding prepares: lay the mixture of the medical stone granule: coral sand particulate quartz sand grains of one layer of particle diameter 5~10mm and the chad of particle diameter about 30mm at the bottom of box for breeding;
(4) aquaculture water: with sea water pump from natural waters or underground sea water well extracting seawater to sand filter, through sand filtration to water tank, with the ClO 2 solution of 0.5ppm, water sterilization is sterilized in water tank, after precipitating 24 hours, enter biofilter, enter back into tank, being squeezed into box for breeding by water circulating pump, keep water level 25~26cm, salinity is 25~32 ‰, pH value 7.5~8.6, water flow velocity 10~30cm/s;
(2) method:
(1) seed put in a suitable place to breed before preparation:
1. equipment and the sterilization of the instrument of use: cultivation bottom, tank wall and all medical stone granule :s, coral sand granule, quartzy sand grains, chad, biofilter, tank material therefor are all cleaned, then with the potassium permanganate solution disinfection of the ClO 2 solution of 20ppm or 20ppm, after placing 4 hours laid again, inject the sea water that filtration treatment is crossed, with the ClO 2 solution of 0.5ppm, water body disinfection is sterilized, ON cycle water pump, allows water circulate sterilization that pipeline is carried out disinfection; After 24 hours, useful vaccine of splashing in filter material for biological filter and box for breeding, open aerator, aquaculture constant temperature machine, temperature is set in 25 ± 2 DEG C, about dissolved oxygen amount 8 mg/litre, minimum cannot be below 5 mg/litre, support water about two days, cultivate probiotics;
2. the cultivation of single-cell algae: carry out single-cell algae cultivation in special box for breeding, the bait of abundant high-quality is prepared for nereis culture, single-cell algae has chrysophyceae, flat algae, pavlova viridis, Nannochloropsis oceanica etc., close water intaking valve, open light compensating lamp, intensity of illumination is 5000~15000 luxs, keeps water temperature 25 DEG C, it is possible to Fast-propagation;
3. the input of the tangleweed such as Entermorpha: the tangleweed 5ppm such as the Entermorpha that will collect from the natural waters intelligent iodine solution containing available iodine 10% soaks disinfection in 30 minutes, then render to equably in box for breeding, water flow velocity is transferred to about 15cm/s, open light compensating lamp, set intensity of illumination as 5000~15000 luxs, accelerate the growth of the tangleweeds such as Entermorpha;
(2) seed is put in a suitable place to breed and daily management:
1. the selection of seed with put in a suitable place to breed: seed require health, not damaged, lovely luster, body surface gloss, size is not limit;Size clam worm branch mailbox is put in a suitable place to breed, breeding density is 50,000 tails~10,000 tails/square meter, along with clam worm individuality increases, need constantly to dredge to support, but cultivation density is not less than 10,000 tails/square meter, gather from seabeach before the seed buied enters box for breeding and to be first put into the 5ppm intelligent iodine solution containing available iodine 10% and soak 5~10 minutes, disinfection;
2. water is changed: owing to adopting circulating water cultivation, the tangleweeds such as Entermorpha are utilized to adhere to culture medium as clam worm, the tangleweeds such as Entermorpha have the effect absorbing breeding water body nutritive salt and increase dissolved oxygen, therefore, breeding process need not change water, only need in box for breeding, add appropriate unicellular algae solution every day;
3. bait feeding: bait throwing in every day 1~2 time, bait be Entermorpha powder and small fish, shrimp, shellfish meat powder broken after the mixture of meat paste, day feeding volume be the 1%~5% of clam worm body weight, visual clam worm situation of ingesting is grasped flexibly; Owing to Entermorpha is the good adherance of clam worm, it is again the bait of clam worm high-quality, should often supplement as required;
4. inflation: high density nereis culture should keep inflating uninterruptedly, keeps in water body about dissolved oxygen amount 8 mg/litre, minimum cannot be below 5 mg/litre;
5. light filling: owing to adopting three-dimensional culture, box for breeding light being blocked to some extent, can affect the growth of the tangleweeds such as Entermorpha and clam worm, therefore, daytime every day to open light compensating lamp, and intensity of illumination is 5000~15000 luxs;
6. daily management: observe clam worm every day and ingest, grow and streamflow regime; Owing to being stamped filter cap on water-level regulator, it can stop the escape of clam worm, but can be blocked by Entermorpha and clam worm cause emitting excessive, it is necessary to note removing; Conscientiously carry out the growth measurement of clam worm, density measurement, provide foundation for bait throwing in, subsequent production management;
7. results: when clam worm individuality grows to more than 100mm, can gather in the crops when specification reaches within 1000/kilogram, first close water intaking valve, open box for breeding bottom row water valve again, put clean aquaculture water, sort out and shake the tangleweeds such as Entermorpha and come off by clam worm, the clam worm adult collected is sorted, the body length box for breeding that returns less than 100mm continues cultivation, long for body more than 100mm specification is reached the commercial insect within 1000/kilogram and carries out packaging sale, the clam worm of the bigger individuality of part is individually chosen as parent for breeding; Clam worm package carrier thing is vermiculite power, and vermiculite power first cools to 18~20 DEG C of moisturizings, fills 5 boxes, every box two-layer, every layer of dress clam worm 1 kilogram for general one case, keeps 18~20 DEG C for ice homoiothermic in case, send airport air transport outlet or domestic; The commercial insect the gathered in the crops sale that namely can live can also drying be sold, and avoids existing clam worm bait and sells the dull and rush season existed, to obtain higher profit;
(3) parent worm is cultivated and artificial breeding:
1. the big individual clam worm individually picked out is put in special box for breeding and carries out hot housing, first close water intaking valve, ice bag or ice chest is put in box for breeding, beginning puts one, melt after thoroughly and put two, by that analogy, artificial gradually reduce the water temperature to about 4 DEG C in box for breeding constant temperature one day by force, slowly heat up every day again, until being raised to 23~24 DEG C, set constant temperature 23 DEG C again, more than 16 DEG C water temperatures are thrown something and fed bait 2~3 times every day, day feeding volume is the 5% of clam worm body weight, bait is Entermorpha powder and small fish, shrimp, the mixture of the meat paste after shellfish meat powder is broken, the prolonging light time promotes parent gonad maturity,
2., when clam worm parent gonad maturity, build there occurs notable change, and in the middle part of clam worm body, parapodum becomes flat, bristle is elongated, clam worm body substantially defines front portion, middle part, rear portion, and hereafter clam worm body is significantly reduced, and body broadens, 2nd district before and after being formed, proparea parapodum is not deformed, and back zone parapodum is flat, and bristle becomes paddle, so far, the abnormal one-tenth heteronereis of clam worm;In order to avoid inbreeding causes degenerate problem, should select, from different box for breeding, the male and female heteronereis that body colour is bright-coloured, mechanical is hindered, energetic, gonad is full after secondary and match; The discrimination method of male and female heteronereis is: male heteronereis a milky white colour band of longitudinal direction occurs at body center back, female inconspicuous, and male heteronereis anal segment has Flos Chrysanthemi shape projection, female then without this feature; First male and female heteronereis branch mailbox is supported several hours temporarily, lay eggs in net cage by what the proportional paired of male and female 7: 1 put into special box for breeding again, male and female heteronereis is known from experience quickly wedding dance and lays eggs, arranges essence, wedding dance is laid eggs, is arranged essence and terminate, pull the dead clam worm sinking to bottom out, wash germ cell several times, keep water temperature 25 ± 2 DEG C, tune up aeration quantity and stir the end, in order to avoid germ cell is sunk; Within about 2 days, just can hatching three and just save nectocaete, the larva just hatched is with internal elgen ball for nutrition, it is not necessary to bait throwing in; After 2 days, paedomorphosis has just saved nectocaete to four, and elgen ball disappears, it is necessary to bait throwing in time, bait is based on single-cell algaes such as marine yeast, chrysophyceae, flat algaes, bait throwing in every day three times, throw something and feed ocean rhodotorula 0.5~1.0mg/L every time, single-cell algae 100,000~200,000 cells/L; When growing to five firm joint nectocaetes, peritroch fades away, travelling reduced capability, sink to the bottom, starting to transfer to the end dwells life of crawling, and at this moment can be properly added the 0.5~1.0mg/L such as Entermorpha powder of throwing something and feeding, chrysophyceae powder, Spirulina powder, and falls case and divide and proceed to larvae cultivation case and continue to cultivate; Larvae cultivation breeding density is 200,000~300,000 tails/square meter, bait throwing in every day 5 times, bait be 80% Entermorpha powder, chrysophyceae powder, Spirulina powder+20% small fish, shrimp, shellfish meat powder broken after meat paste, daily ration, feeding quantity 3~5 grams per square meter; Growth along with children's clam worm body length, emerging at any time point pool cultivated, children's clam worm body length grows to 2~3cm, and namely 11 just more than joint, breeding density is 50,000~10,000/square meter, bait throwing in every day 1~2 time, bait be Entermorpha powder and small fish, shrimp, shellfish meat powder broken after the mixture of meat paste, day feeding volume be the 1%~5% of clam worm body weight, visual clam worm situation of ingesting is grasped flexibly, owing to Entermorpha is the good adherance of clam worm, it is again the bait of clam worm high-quality, should often supplement as required.
(4) suggestion:
1. the condition required with a lot of aquacultures overlaps, if Eriocheir sinensis water temperature is when 15-26 DEG C, food ration is big, grow the fastest, clam worm optimum growth temp is 16~32 DEG C, salinity is 25~32 ‰, pH value 7.5~8.6, therefore multi items aquatic products can be allowed to hold concurrently support simultaneously, cultivate again clam worm as supported Eriocheir sinensis temporarily simultaneously, the optimum water temperature 24 ± 2 DEG C of they growths it is set in aquaculture constant temperature machine, although being Relationships between Natural Enemies between them, but they are in respective box for breeding, it is independent of each other, clam worm fed by the residual bait of available Eriocheir sinensis again, Eriocheir sinensis is fed with dead clam worm and disability clam worm, both the waste of bait had been reduced, high economic benefit can be obtained again.
2. this method is used for cultivating red clam worm, but is not limited to cultivate red clam worm, it is also possible in the clam worm cultivating other kind.