CN108770742A - A kind of method for culturing seedlings of Macrobrachium rosenbergii high-survival rate - Google Patents
A kind of method for culturing seedlings of Macrobrachium rosenbergii high-survival rate Download PDFInfo
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- CN108770742A CN108770742A CN201810520393.7A CN201810520393A CN108770742A CN 108770742 A CN108770742 A CN 108770742A CN 201810520393 A CN201810520393 A CN 201810520393A CN 108770742 A CN108770742 A CN 108770742A
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- 241001327110 Macrobrachium rosenbergii Species 0.000 title claims abstract description 23
- 238000000034 method Methods 0.000 title claims abstract description 20
- 238000012258 culturing Methods 0.000 title claims abstract description 19
- 241000238557 Decapoda Species 0.000 claims abstract description 74
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 28
- 230000001418 larval effect Effects 0.000 claims abstract description 12
- 210000001015 abdomen Anatomy 0.000 claims abstract description 10
- 230000004720 fertilization Effects 0.000 claims abstract description 10
- 210000001161 mammalian embryo Anatomy 0.000 claims abstract description 10
- 230000034004 oogenesis Effects 0.000 claims abstract description 10
- 238000009395 breeding Methods 0.000 claims abstract description 6
- 230000001488 breeding effect Effects 0.000 claims abstract description 6
- 230000013011 mating Effects 0.000 claims abstract description 6
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims description 16
- 241000595940 Notostraca Species 0.000 claims description 16
- 241000133262 Nauplius Species 0.000 claims description 14
- 235000011950 custard Nutrition 0.000 claims description 9
- 229920001661 Chitosan Polymers 0.000 claims description 8
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 claims description 8
- 229930003268 Vitamin C Natural products 0.000 claims description 8
- 235000019154 vitamin C Nutrition 0.000 claims description 8
- 239000011718 vitamin C Substances 0.000 claims description 8
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 5
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 5
- 229910052760 oxygen Inorganic materials 0.000 claims description 5
- 239000001301 oxygen Substances 0.000 claims description 5
- 238000013327 media filtration Methods 0.000 claims description 4
- 238000004659 sterilization and disinfection Methods 0.000 claims description 4
- ZKQDCIXGCQPQNV-UHFFFAOYSA-N Calcium hypochlorite Chemical compound [Ca+2].Cl[O-].Cl[O-] ZKQDCIXGCQPQNV-UHFFFAOYSA-N 0.000 claims description 3
- 239000007844 bleaching agent Substances 0.000 claims description 3
- 230000004083 survival effect Effects 0.000 abstract description 6
- 230000006978 adaptation Effects 0.000 abstract description 4
- 239000013505 freshwater Substances 0.000 abstract description 4
- 230000000052 comparative effect Effects 0.000 description 4
- 230000000366 juvenile effect Effects 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 235000013406 prebiotics Nutrition 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 1
- 241000238017 Astacoidea Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- NIPNSKYNPDTRPC-UHFFFAOYSA-N N-[2-oxo-2-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)ethyl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(CNC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 NIPNSKYNPDTRPC-UHFFFAOYSA-N 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 238000004061 bleaching Methods 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 239000003610 charcoal Substances 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- 235000015170 shellfish Nutrition 0.000 description 1
- 239000004575 stone Substances 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/50—Culture of aquatic animals of shellfish
- A01K61/59—Culture of aquatic animals of shellfish of crustaceans, e.g. lobsters or shrimps
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The present invention relates to a kind of method for culturing seedlings of Macrobrachium rosenbergii high-survival rate, it includes the following steps：（a）Multiple nursery ponds are injected after river water is sterilized；（b）It is put into the female shrimp of selection and breeding into any one of nursery pond and male shrimp is cultivated and makes its mating, fertilization；（c）The female shrimp of the oogenesis of after fertilization is transferred in another described nursery pond and is cultivated；When the color of the female shrimp abdomen embryo of oogenesis becomes light grey or grey, fall off to obtain the shrimp seedling young；（d）The shrimp seedling young that strainer is fished for is transferred to wherein by the salinity for controlling water in another described nursery pond, and it is 8 ~ 120,000 tails/m to control density3；The salinity of water starts to be 6 ~ 7 ‰ in the nursery pond, and 4 ~ 5 ‰ are reduced to after cultivating 6 ~ 7 days, and 2.5 ~ 3 ‰ are reduced to after cultivating 12 ~ 13 days, and 1 ~ 2 ‰ are reduced to after cultivating 18 ~ 19 days；（e）It will emerge after the shrimp seedling larval culture 20 ~ 22 days.Can improve in this way the shrimp seedling young to the adaptation of fresh water can, to improve the survival rate of Macrobrachium rosenbergii；And cost can be reduced.
Description
Technical field
The invention belongs to aquatic products nursery fields, are related to a kind of method for culturing seedlings of Macrobrachium rosenbergii, and in particular to a kind of Roche
The method for culturing seedlings of pond crayfish high-survival rate.
Background technology
Aquatic products refer to animal and its converted products of ocean and fresh water fishery production.Fresh and living aquatic products, be divided into fish, shrimp,
Crab, four major class of shellfish.Aquaculture is the production activity of the lower breeding of artificial control, cultivation and harvest aquatic animals and plants, and aquatic products is supported
It is typically to raise the aquatic products seedling of purchase to certain size and sell to grow family.Aquatic products are needed in cultivation using prebiotic
Bacterium, vitamin etc. are to improve the resistance of aquatic products, harmful microbe in pool is inhibited to breed.Existing prebiotic promotor composition
After being spilled into pool, it is easy to cause the drastically decline of the transparency and finish of water body, this can influence the growth and development of aquatic products.
Invention content
The technical problem to be solved in the invention is a kind of Macrobrachium rosenbergii high-survival rate proposed in view of the above technical problems
Method for culturing seedlings.
In order to achieve the above objectives, the technical solution adopted in the present invention is：A kind of nursery side of Macrobrachium rosenbergii high-survival rate
Method, it includes the following steps：
（a）Multiple nursery ponds are injected after river water is sterilized；
（b）It is put into the female shrimp of selection and breeding into any one of nursery pond and male shrimp is cultivated and makes its mating, fertilization；
（c）The female shrimp of the oogenesis of after fertilization is transferred in another described nursery pond and is cultivated；Wait for the female shrimp abdomen embryo of oogenesis
Color when becoming light grey or grey, fall off to obtain the shrimp seedling young；
（d）The shrimp seedling young that strainer is fished for is transferred to wherein, and controls by the salinity for controlling water in another described nursery pond
Density processed is 8 ~ 120,000 tails/m3；Fairy shrimp nauplius is fed, 4 ~ 8 h are fed once；By the shrimp seedling larval culture 7 ~ 10
After it, increases before feeding fairy shrimp nauplius and feed egg custard；After the shrimp seedling larval culture 15 ~ 18 days, also increase
Feed prawn slice；The feeding volume of fairy shrimp nauplius, egg custard and prawn slice is subject to the shrimp seedling young 1h and is eaten up；It is described to educate
The salinity of water starts to be 6 ~ 7 ‰ in Miao Chi, and 4 ~ 5 ‰ are reduced to after cultivating 6 ~ 7 days, and 2.5 ~ 3 ‰ are reduced to after cultivating 12 ~ 13 days, cultivates
1 ~ 2 ‰ are reduced to after 18 ~ 19 days；
（e）It will emerge after the shrimp seedling larval culture 20 ~ 22 days；
Step（c）, step（d）And step（e）In, water temperature is 31 ~ 32 DEG C in the nursery pond, and dissolved oxygen is 5.5 ~ 5.8mg/L.
Optimally, step（a）In, pool processed is dug at the place of bordering on the river, so that river water is flowed into after media filtration in the pool, often
Cubic meter is carried out disinfection with 20 ~ 50g bleaching powder, injects multiple nursery ponds after standing 7 ~ 10 days；The medium is to contain sea
Continuous, activated carbon and sandstone mixture.
Optimally, step（c）In, the female shrimp for sloughing abdomen embryo is put into step（b）In the nursery pond, make it
It mates with male shrimp.
Optimally, step（d）In, the strainer is 160 ~ 180 mesh.
Optimally, step（d）In, during only feeding fairy shrimp nauplius, also it is separately added into the dimension of 500 ~ 1000ppm
Raw element C and chitosan.
Since above-mentioned technical proposal is used, the present invention has following advantages compared with prior art：Macrobrachium rosenbergii of the present invention
The method for culturing seedlings of high-survival rate, by synchronizing the adjustment of the adjustment and bait that carry out salinity during shrimp seedling larval culture, this
Sample can improve the shrimp seedling young to the adaptation of fresh water can, to improve the survival rate of Macrobrachium rosenbergii；And cost can be reduced.
Specific implementation mode
The method for culturing seedlings of Macrobrachium rosenbergii high-survival rate of the present invention, it includes the following steps：（a）It is injected after river water is sterilized more
A nursery pond；（b）Be put into the female shrimp of selection and breeding into any one of nursery pond and male shrimp cultivated and made its mating, by
Essence；（c）The female shrimp of the oogenesis of after fertilization is transferred in another described nursery pond and is cultivated；Wait for the female shrimp abdomen embryo's of oogenesis
When color becomes light gray or grey, fall off to obtain the shrimp seedling young；（d）The salinity for controlling water in another described nursery pond, will filter
The shrimp seedling young that net is fished for is transferred to wherein, and it is 8 ~ 120,000 tails/m to control density3；Feed fairy shrimp nauplius, 4 ~ 8
H is fed once；After the shrimp seedling larval culture 7 ~ 10 days, increases before feeding fairy shrimp nauplius and feed egg custard；
After the shrimp seedling larval culture 15 ~ 18 days, also increases and feed prawn slice；Fairy shrimp nauplius, egg custard and prawn slice are fed
Amount is subject to the shrimp seedling young 1h and is eaten up；The salinity of water starts to be 6 ~ 7 ‰ in the nursery pond, it is reduced to 4 after cultivating 6 ~ 7 days ~
5 ‰, it is reduced to 2.5 ~ 3 ‰ after cultivating 12 ~ 13 days, 1 ~ 2 ‰ are reduced to after cultivating 18 ~ 19 days；（e）By the shrimp seedling larval culture 20 ~
It emerges after 22 days；Step（c）, step（d）And step（e）In, water temperature is 31 ~ 32 DEG C in the nursery pond, dissolved oxygen 5.5
~5.8mg/L.Can improve in this way the shrimp seedling young to the adaptation of fresh water can, to improve the survival rate of Macrobrachium rosenbergii；And energy
Enough reduce cost.
Step（a）In, pool processed is dug at the place of bordering on the river, river water is made to be flowed into after media filtration in the pool, every cubic metre
It is carried out disinfection with 20 ~ 50g bleaching powder, injects multiple nursery ponds after standing 7 ~ 10 days；The medium is to contain sponge, activity
The mixture of charcoal and sandstone.The cultivation of seedling is directly carried out with river water, can also improve adaptation energy of the shrimp seedling young to river water in this way
Power, at the same it is at low cost.Step（c）In, the female shrimp for sloughing abdomen embryo is put into step（b）In the nursery pond, make it
It mates with male shrimp；The usually cycle fertilization 5 ~ 6 times in this way of above-mentioned female shrimp, can make full use of physical strong female in this way
Shrimp advantageously reduces cost and ensures that seedling has good gene.Step（d）In, the strainer is 160 ~ 180 mesh；Step
（d）In, during only feeding fairy shrimp nauplius, it is also separately added into the vitamin C and chitosan of 500 ~ 1000ppm, this is
Because vitamin C and chitosan can not only purify water, and they can improve the constitution of the shrimp seedling young, improve its resistance
Power further increases the survival rate of its raising.
The preferred embodiment of the invention will be described in detail below.
Embodiment 1
The present embodiment provides a kind of method for culturing seedlings of Macrobrachium rosenbergii high-survival rate, it includes the following steps：
（a）Pool processed is dug at the place of bordering on the river, river water is made to be flowed into pool after media filtration（Medium by set gradually sand stone layer,
Active carbon layer and spongy layer composition）, stop when the water in pool is noted to the 2/3 of volume；With every cubic metre of use 20 ~ 50g bleaching
The dosage of powder carries out disinfection, and injects four nursery ponds after standing 7 ~ 10 days；
（b）It is put into the female shrimp of selection and breeding into first nursery pond and male shrimp is cultivated and makes its mating, fertilization（It specifically refers to
Application No. is the embodiments 1 of 201711085858 .2 Chinese invention patents）；
（c）The female shrimp of the oogenesis of after fertilization is transferred in first nursery pond and is cultivated（It is 31 ~ 32 to control water temperature in nursery pond
DEG C, dissolved oxygen is 5.5 ~ 5.8mg/L）；When the color of the female shrimp abdomen embryo of oogenesis becomes light grey or grey, embryo is from female shrimp abdomen
Portion falls off to obtain the shrimp seedling young；The female shrimp for sloughing abdomen embryo is placed into step（b）In the nursery pond, carried out again with male shrimp
Mating（It is 5 ~ 6 times/year repeatable）.
（d）The salinity for controlling water in third nursery pond is 7 ‰（In nursery pond water temperature also be 31 ~ 32 DEG C, dissolved oxygen be 5.5 ~
5.8mg/L）, the shrimp seedling young fished for the strainer of 160 ~ 180 mesh is simultaneously transferred to wherein, control cultivation density be 8 ~ 120,000 tails/
m3；It is separately added into the vitamin C and chitosan of 500ppm, feeds fairy shrimp nauplius, 4 ~ 8 h are fed once；It cultivates 6 days
The salinity of water in nursery pond is reduced to 5 ‰ afterwards, is continued after cultivating 1 day, increases before feeding fairy shrimp nauplius and feeds egg
Custard（Egg custard and the mass ratio of fairy shrimp are 1:1, feeding volume is subject to shrimp seedling young 1h and is eaten up, similarly hereinafter）；Continue to cultivate 5 days,
Salinity is reduced to 3 ‰；Again after 3 days, also increases and feed prawn slice（Prawn slice, egg custard and the mass ratio of fairy shrimp are 1:1:1）；3 days again
Afterwards, salinity is reduced to 13 ‰；
（e）It emerges after shrimp seedling larval culture is added up to 20 days.
Embodiment 2
The present embodiment provides a kind of method for culturing seedlings of Macrobrachium rosenbergii high-survival rate, it with it is almost the same in embodiment 1, it is different
It is：Step（d）In, the vitamin C and chitosan of addition are respectively 1000ppm.
Embodiment 3
The present embodiment provides a kind of method for culturing seedlings of Macrobrachium rosenbergii high-survival rate, it with it is almost the same in embodiment 1, it is different
It is：Step（d）In, the vitamin C and chitosan of addition are respectively 800ppm.
Comparative example 1
This example provides a kind of method for culturing seedlings of Macrobrachium rosenbergii, it with it is almost the same in embodiment 1, unlike：Step（d）In
Not carrying out the gradient of salinity reduces；But in step（e）In, it emerges after being desalinated.
Comparative example 2
This example provides a kind of method for culturing seedlings of Macrobrachium rosenbergii, it with it is almost the same in embodiment 1, unlike：Step（d）
In, the vitamin C that is only added.
Comparative example 3
This example provides a kind of method for culturing seedlings of Macrobrachium rosenbergii, it with it is almost the same in embodiment 1, unlike：Step（d）
In, the vitamin C and chitosan that are not added.
The survival rate of Macrobrachium rosenbergii seedling in embodiment 1-3, comparative example 1-3 is measured, survival rate=（Total juvenile prawn
The dead juvenile prawn number of number-）/ total juvenile prawn number × 100%, respectively 94.5%, 94.0%, 96.0%, 92.5%, 92.0% and 90%.
The above embodiments merely illustrate the technical concept and features of the present invention, and its object is to allow person skilled in the art
Scholar cans understand the content of the present invention and implement it accordingly, and it is not intended to limit the scope of the present invention.It is all according to the present invention
Equivalent change or modification made by Spirit Essence, should be covered by the protection scope of the present invention.
Claims (5)
1. a kind of method for culturing seedlings of Macrobrachium rosenbergii high-survival rate, which is characterized in that it includes the following steps：
（a）Multiple nursery ponds are injected after river water is sterilized；
（b）It is put into the female shrimp of selection and breeding into any one of nursery pond and male shrimp is cultivated and makes its mating, fertilization；
（c）The female shrimp of the oogenesis of after fertilization is transferred in another described nursery pond and is cultivated；Wait for the female shrimp abdomen embryo of oogenesis
Color when becoming light grey or grey, fall off to obtain the shrimp seedling young；
（d）The shrimp seedling young that strainer is fished for is transferred to wherein, and controls by the salinity for controlling water in another described nursery pond
Density processed is 8 ~ 120,000 tails/m3；Fairy shrimp nauplius is fed, 4 ~ 8 h are fed once；By the shrimp seedling larval culture 7 ~ 10
After it, increases before feeding fairy shrimp nauplius and feed egg custard；After the shrimp seedling larval culture 15 ~ 18 days, also increase
Feed prawn slice；The feeding volume of fairy shrimp nauplius, egg custard and prawn slice is subject to the shrimp seedling young 1h and is eaten up；It is described to educate
The salinity of water starts to be 6 ~ 7 ‰ in Miao Chi, and 4 ~ 5 ‰ are reduced to after cultivating 6 ~ 7 days, and 2.5 ~ 3 ‰ are reduced to after cultivating 12 ~ 13 days, cultivates
1 ~ 2 ‰ are reduced to after 18 ~ 19 days；
（e）It will emerge after the shrimp seedling larval culture 20 ~ 22 days；
Step（c）, step（d）And step（e）In, water temperature is 31 ~ 32 DEG C in the nursery pond, and dissolved oxygen is 5.5 ~ 5.8mg/L.
2. the method for culturing seedlings of Macrobrachium rosenbergii high-survival rate according to claim 1, it is characterised in that：Step（a）In, it is bordering on the river
Pool processed is dug at place, so that river water is flowed into after media filtration in the pool, every cubic metre is carried out disinfection with 20 ~ 50g bleaching powder, quiet
Multiple nursery ponds are injected after setting 7 ~ 10 days；The medium is the mixture containing sponge, activated carbon and sandstone.
3. the method for culturing seedlings of Macrobrachium rosenbergii high-survival rate according to claim 1, it is characterised in that：Step（c）In, it will slough
The female shrimp of abdomen embryo is put into step（b）In the nursery pond, it is made to mate with male shrimp.
4. the method for culturing seedlings of Macrobrachium rosenbergii high-survival rate according to claim 1, it is characterised in that：Step（d）In, the filter
Net is 160 ~ 180 mesh.
5. the method for culturing seedlings of Macrobrachium rosenbergii high-survival rate according to claim 1, it is characterised in that：Step（d）In, only throwing
During feeding fairy shrimp nauplius, it is also separately added into the vitamin C and chitosan of 500 ~ 1000ppm.
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| CN112056245A (en) * | 2020-10-09 | 2020-12-11 | 浙江省淡水水产研究所 | Creating process and hatching device for SPF (specific pathogen free) larvae of macrobrachium rosenbergii |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109479778A (en) * | 2018-12-08 | 2019-03-19 | 海南海壹水产种苗有限公司 | A kind of evaluation method of Penaeus Vannmei shrimp seedling anti-stress ability |
| CN109479778B (en) * | 2018-12-08 | 2021-06-15 | 海南海壹水产种苗有限公司 | Method for evaluating anti-stress capability of young penaeus vannamei boone |
| CN112056245A (en) * | 2020-10-09 | 2020-12-11 | 浙江省淡水水产研究所 | Creating process and hatching device for SPF (specific pathogen free) larvae of macrobrachium rosenbergii |
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