CN109479778A - A kind of evaluation method of Penaeus Vannmei shrimp seedling anti-stress ability - Google Patents
A kind of evaluation method of Penaeus Vannmei shrimp seedling anti-stress ability Download PDFInfo
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- CN109479778A CN109479778A CN201811498610.3A CN201811498610A CN109479778A CN 109479778 A CN109479778 A CN 109479778A CN 201811498610 A CN201811498610 A CN 201811498610A CN 109479778 A CN109479778 A CN 109479778A
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- 241000238557 Decapoda Species 0.000 title claims abstract description 112
- 238000011156 evaluation Methods 0.000 title claims abstract description 20
- 230000002180 anti-stress Effects 0.000 title claims abstract description 17
- 241000927735 Penaeus Species 0.000 title claims abstract description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 86
- 230000004083 survival effect Effects 0.000 claims abstract description 23
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 19
- 229910052760 oxygen Inorganic materials 0.000 claims description 19
- 239000001301 oxygen Substances 0.000 claims description 19
- 230000036541 health Effects 0.000 claims description 10
- 238000001514 detection method Methods 0.000 claims description 8
- 208000002173 dizziness Diseases 0.000 claims description 6
- 229920003023 plastic Polymers 0.000 claims description 6
- 239000004033 plastic Substances 0.000 claims description 6
- 230000006641 stabilisation Effects 0.000 claims description 4
- 238000011105 stabilization Methods 0.000 claims description 4
- 239000013505 freshwater Substances 0.000 claims description 2
- 230000009191 jumping Effects 0.000 claims description 2
- 208000035240 Disease Resistance Diseases 0.000 abstract description 5
- 238000000034 method Methods 0.000 description 4
- 150000003839 salts Chemical class 0.000 description 4
- 241000238553 Litopenaeus vannamei Species 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 238000012790 confirmation Methods 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 210000000697 sensory organ Anatomy 0.000 description 2
- 241000530454 Litopenaeus schmitti Species 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/50—Culture of aquatic animals of shellfish
- A01K61/59—Culture of aquatic animals of shellfish of crustaceans, e.g. lobsters or shrimps
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The present invention relates to a kind of evaluation methods, especially a kind of evaluation method of Penaeus Vannmei shrimp seedling anti-stress ability, the evaluation method includes detecting to the water body in shrimp seedling source, record the cultivation number of days of shrimp seedling, adjust the water quality of test, PL5 shrimp seedling resisting stress survival rate is tested, PL5 shrimp seedling resisting stress survival rate is calculated；PL10 shrimp seedling resisting stress survival rate is tested, PL10 shrimp seedling resisting stress survival rate is calculated；The present invention calculates shrimp seedling survival rate by water salinity difference to react fry quality difference, the anti-stress ability for objectively having reacted Penaeus Vannmei shrimp seedling is strong and weak, strong foundation is provided for fry quality selection, and strong data foundation can also be provided to the adaptability and later period disease resistance for converting cultivating condition, seed is selected for raiser, and strong foundation is provided.
Description
Technical field
The present invention relates to a kind of evaluation method, especially a kind of evaluation method of Penaeus Vannmei shrimp seedling anti-stress ability.
Background technique
Penaeus Vannmei, scientific name litopenaeus vannamei are excellent because its growth is fast, disease resistance is strong, thermophilic, suitable salt, range are wide etc.
Point becomes the hot spot cultivated at present.Domestic nursery is various in style at present more than 600,000,000,000 tails；Wherein, a generation for external import
Seedling accounts for main advantage；Domestic two generation seedlings, Duo Daimiao also have the characteristics that certain.
It is increasingly severe with domestic breeding environment, the quality of fry quality distinguish it is increasingly apparent, due between each seed
Qualitative characteristics it is different, how to select seed become raiser matter of utmost importance be also problem.Embodiment seed quality quality has more
Aspect factor, such as speed of growth, the uniformity, anti-stress ability etc., especially prominent feature are the powers of anti-stress ability, anti-to answer
The power for the ability of swashing is directly related to disease resistance, but raiser tends to rely on when selecting seed and visually observes, naked eyes
The size and body colour of seedling are only intuitively reacted in observation, and have no way of learning to its actual disease resistance.Therefore, it is badly in need of one kind at present
It is capable of the method for validity test and evaluation Penaeus Vannmei shrimp seedling anti-stress ability, raiser is allow effectively to judge shrimp seedling
Quality.
Culture of Penaeus vannamei range sweeps the country, but various regions water quality has greatest differences again, and seedling water is also gradually light
Change, salinity stress directly test the adaptability of seed, as an important indicator for judging shrimp seedling anti-stress ability.
Summary of the invention
In view of the deficiencies of the prior art, the present invention provides a kind of evaluation method of Penaeus Vannmei shrimp seedling anti-stress ability,
Pass through the evaluation to Penaeus Vannmei shrimp seedling anti-stress ability, directly differentiation shrimp seedling quality grade.
The technical solution of the present invention is as follows:
A kind of evaluation method of Penaeus Vannmei shrimp seedling anti-stress ability, it is characterised in that: the evaluation method include with
Lower step:
Step A: detecting the water body in shrimp seedling source, and the index of detection includes salinity, water temperature and appearance oxygen；
Step B: recording the cultivation number of days of shrimp seedling, and it is 14-15 days that PL5 shrimp seedling, which cultivates number of days, and PL10 shrimp seedling cultivation number of days is
19-20 days；
Step C: adjust test water quality, water quality is transparent sterile, PL5 shrimp seedling stress water quality be salinity 15ppt, salinity
Gradient difference > 7ppt, total alkalinity 150-180mg/L, water temperature are 29-30 DEG C, dissolved oxygen > 5mg/L；PL10 shrimp seedling stress water quality salt
Degree be 0ppt and 30ppt, total alkalinity 150-180mg/L, 29-30 DEG C of water temperature, dissolved oxygen > 5mg/L；
Step D: test PL5 shrimp seedling resisting stress survival rate prepares the white jar of plastics of 1L capacity, and jar bore is 10cm,
The test water 1L of ready PL5 shrimp seedling is added in a height of 13cm, and taking the PL5 shrimp seedling of 100 tails health to be put into stress be suitable in water
Whether hour hands rotate water body, and shrimp seedling is made to be evenly distributed, go through water whirlpool center and have and sink to the bottom or dizzy shrimp seedling, if any dead seedling
Or Bai Miaoxu is pressed from both sides out with tweezers, and fills the health young shrimp of respective numbers；Lid is gently put on the white jar of plastics, prevents shrimp
Seedling is jumped out, and certain gap is retained, not salable, opens lid after timing 1h, 1h, and the shrimp seedling being sticked on lid is put back to water
In, rotate clockwise water body, after waiting waterflow stabilization, intolerant to stress be dead shrimp seedling can sink to bottom center, check bottom shrimp
The The dead quantity of seedling；
Step E: PL5 shrimp seedling resisting stress survival rate is calculated；Calculation formula are as follows: PL5 shrimp seedling resisting stress survival rate=(100- is dead
Die quantity)/100*100%；
Step F: test PL10 shrimp seedling resisting stress survival rate prepares the white jar of plastics of two 1L capacity, jar bore is
10cm, a height of 13cm, first jar water salinity are 0ppt, and pure fresh water, second jar water salinity is 30ppt, it is ensured that
Clean water quality, other indexs are identical as described in step C；It takes the PL10 shrimp seedling of 100 tails health to be put into first jar, revolves clockwise
Turn water body, shrimp seedling is made to be evenly distributed, go through water whirlpool center and whether have and sink to the bottom or dizzy shrimp seedling, if any dead seedling or Bai Miao
It need to be pressed from both sides out with tweezers, and fill the health young shrimp of respective numbers, timing 30 minutes；All shrimp seedlings are turned by dredger after 30 minutes
Second jar is moved on to, water body is rotated clockwise, shrimp seedling is made to be evenly distributed, goes through whether water whirlpool center sinks to the bottom or swoon
Dizzy shrimp seedling is pressed from both sides out with tweezers if any dead seedling or Bai Miaoxu, and fills the health young shrimp of respective numbers, and the timing 30 that closes the lid divides
Clock；Lid is opened after 30 minutes, the shrimp seedling being sticked on lid is put back in water, rotates clockwise water body, after waiting waterflow stabilization,
Intolerant to stress dead shrimp seedling can sink to bottom center, check the The dead quantity of bottom seedling；
Step G: PL10 shrimp seedling resisting stress survival rate is calculated；Calculation formula are as follows: PL10 shrimp seedling resisting stress survival rate=(100-
The dead quantity)/100*100%；
Step H: evaluation criterion: in quality equal PL5 shrimp seedling survival rate PL10 shrimp seedling equal on 90% or more, quality at
Motility rate is 85% or more.
Further, the index normal range (NR) of detection described in step A is salinity 15-30ppt, 29-30 DEG C of water temperature, holds oxygen 5-
8mg/L；
Further, poor ± 1 DEG C of the temperature gradient of PL5 shrimp seedling water temperature described in step C, the temperature ladder of PL10 shrimp seedling water temperature
Spend poor ± 1 DEG C.
The invention has the benefit that
The present invention calculates shrimp seedling survival rate by water salinity difference to react fry quality difference, has objectively reacted South America
The anti-stress ability of white shrimp shrimp seedling is strong and weak, provides strong foundation for fry quality selection, and can also be to transformation cultivating condition
Adaptability and later period disease resistance strong data foundation is provided, select seed for raiser and strong foundation be provided.
Specific embodiment
Specific embodiments of the present invention will be further explained below:
Embodiment 1:
A kind of evaluation method of Penaeus Vannmei shrimp seedling anti-stress ability, the evaluation method the following steps are included:
1) water body in shrimp seedling source is detected, the index of detection includes salinity, water temperature and appearance oxygen；
2) the cultivation number of days of shrimp seedling is recorded, it is 14-15 days that PL5 shrimp seedling, which cultivates number of days, and it is 19-20 that PL10 shrimp seedling, which cultivates number of days,
It；
3) adjust test water quality, water quality is transparent sterile, PL5 shrimp seedling stress water quality be salinity 15ppt, salinity gradient
Difference > 7ppt, total alkalinity 150-180mg/L, water temperature are 29-30 DEG C, dissolved oxygen > 5mg/L；PL10 shrimp seedling stress Water salinity be
0ppt and 30ppt, total alkalinity 150-180mg/L, 29-30 DEG C of water temperature, dissolved oxygen > 5mg/L；
4) PL5 shrimp seedling resisting stress survival rate is tested, prepares the white jar of plastics of 1L capacity, jar bore is 10cm, a height of
The test water 1L of ready PL5 shrimp seedling is added in 13cm, and taking the PL5 shrimp seedling of 100 tails health to be put into stress be in water, clockwise
Water body is rotated, shrimp seedling is made to be evenly distributed, water whirlpool center is gone through and whether has and sink to the bottom or dizzy shrimp seedling, if any dead seedling or white
Seedling need to be pressed from both sides out with tweezers, and fill the health young shrimp of respective numbers；Lid is gently put on the white jar of plastics, prevents shrimp seedling from jumping
Out, retain certain gap, it is not salable, lid is opened after timing 1h, 1h, the shrimp seedling being sticked on lid is put back in water, it is suitable
Hour hands rotate water body, after waiting waterflow stabilization, intolerant to stress dead shrimp seedling can sink to bottom center, check the dead of bottom shrimp seedling
Die quantity；
5) PL5 shrimp seedling resisting stress survival rate is calculated；Calculation formula are as follows: PL5 shrimp seedling resisting stress survival rate=(100- death toll
Amount)/100*100%；
6) PL10 shrimp seedling resisting stress survival rate to be tested, the white jar of plastics of two 1L capacity is prepared, jar bore is 10cm,
A height of 13cm, first jar water salinity are 0ppt, and pure fresh water, second jar water salinity is 30ppt, it is ensured that water quality is clean
Only, other indexs are identical as described in step C；It takes the PL10 shrimp seedling of 100 tails health to be put into first jar, rotates clockwise water
Whether body makes shrimp seedling be evenly distributed, go through water whirlpool center and have and sink to the bottom or dizzy shrimp seedling, uses if any dead seedling or Bai Miaoxu
Tweezers press from both sides out, and fill the health young shrimp of respective numbers, and timing 30 minutes；All shrimp seedlings are transferred to by dredger after 30 minutes
Whether second jar, rotates clockwise water body, shrimp seedling is made to be evenly distributed, go through water whirlpool center and sink to the bottom or dizzy
Shrimp seedling is pressed from both sides out with tweezers if any dead seedling or Bai Miaoxu, and fills the health young shrimp of respective numbers, is closed the lid timing 30 minutes；
Lid is opened after 30 minutes, the shrimp seedling being sticked on lid is put back in water, rotates clockwise water body, after waiting waterflow stabilization, no
Resistance to shrimp seedling that stress be dead can sink to bottom center, check the The dead quantity of bottom seedling；
7) PL10 shrimp seedling resisting stress survival rate is calculated；Calculation formula are as follows: PL10 shrimp seedling resisting stress survival rate=(100- is dead
Quantity)/100*100%；
8) evaluation criterion: the PL10 shrimp seedling survival rate that equal PL5 shrimp seedling survival rate waits on 90% or more, quality in quality
85% or more.
Wherein, the index normal range (NR) of detection described in step A is salinity 15-30ppt, 29-30 DEG C of water temperature, holds oxygen 5-8mg/
L；
Poor ± 1 DEG C of the temperature gradient of PL5 shrimp seedling water temperature described in step C, the temperature gradient of PL10 shrimp seedling water temperature poor ± 1
℃。
Embodiment 2:
Certain the seedling field workshop A nursery 16,000,000 cultivates 14 days, 8 ponds (No. 1-8#), each water body 13T, every pond seedling taking
300-500 tail is packaged.
(1) number of days 14 days determining seedling stages were cultivated as P5 shrimp seedling according to it；
(2) to the temperature of water, salinity, dissolved oxygen is measured, and measuring its temperature is 30 DEG C, salinity 30ppt, and dissolved oxygen is
5mg/L, total alkalinity 146mg/L, water quality data illustrate that aquatic environment is more normal；
(3) test water is got out, 1L/ tank, totally 8 tank, tests the water quality survey of water, and salinity 15ppt is 29.5 DEG C of temperature, molten
Oxygen 6mg/L, total alkalinity 152mg/L；
(4) respective labels are sticked by workshop pond number to each jar, each 100 normal shrimp seedling of shape of tail state of sample picking is put into
The jar of respective labels, gently closes the lid, and starts timing；
(5) after 1h, lid is opened, gently rotates water body clockwise with glass bar, to the water flows gently, calculating rests on stress
The shrimp seedling quantity of confirmation death among jar, and record one by one, obtain the seedling field workshop the A pond the 1-8 resisting stress situation.As a result
As shown in table 1.
Table 1: the workshop certain nursery A pond 1-8 resisting stress situation record
| Workshop | Chi Hao | Stage | Strain | Detection time | Seedling lake salt degree (‰) | Salinity stress percentage for 15 ‰ |
| A | 1# | P5 | It is day and night fast | 2018.4.28 | 30 | 98% |
| A | 2# | P5 | It is day and night fast | 2018.4.28 | 30 | 99% |
| A | 3# | P5 | It is day and night fast | 2018.4.28 | 30 | 31% |
| A | 4# | P5 | It is day and night fast | 2018.4.28 | 30 | 84% |
| A | 5# | P5 | It is day and night fast | 2018.4.28 | 30 | 52% |
| A | 6# | P5 | It is day and night fast | 2018.4.28 | 30 | 94% |
| A | 7# | P5 | It is day and night fast | 2018.4.29 | 30 | 61% |
| A | 8# | P5 | It is day and night fast | 2018.4.29 | 30 | 96% |
Even if table 1 is the result shows that shrimp seedling quality is in the same workshop, the ability of resisting stress is also different, from 31% to
99% differs, if but only distinguished from sense organ, naked eyes be difficult to distinguish really, salinity of the present invention stress method be shrimp seedling quality
Selection provide effective reference.
Embodiment 3:
Certain the seedling field workshop B nursery 8,000,000 cultivates 19 days, 4 ponds (pond 3-6#), each water body 13T, every pond seedling taking
300-500 tail is packaged.
(1) number of days 18 days determining seedling stages are cultivated as P10 shrimp seedling according to it；
(2) to the temperature of water, salinity, dissolved oxygen is measured, and measuring its temperature is 30 DEG C, salinity 24ppt, and dissolved oxygen is
4.5mg/L, total alkalinity 140mg/L, water quality data illustrate that aquatic environment is more normal；
(3) battery of tests water is got out, 1L/ tank, totally 4 tank, water salinity 0ppt, 29.5 DEG C of temperature, dissolved oxygen 5.8mg/
L, total alkalinity 153mg/L；
(4) respective labels are sticked by workshop pond number to each jar, each 100 normal shrimp seedling of shape of tail state of sample picking is put into
The jar of first group of respective labels, gently closes the lid, timing 30min；
(5) prepare second group of test water, 1L/ tank, totally 4 tank, water salinity 30ppt, 29.5 DEG C of temperature, dissolved oxygen 5.7mg/L,
Total alkalinity 151mg/L；
(6) after 30min, 100 tail seedlings in above-mentioned (4) are transferred to second group of test stress be in tank, timing 30min again,
(7) lid is opened after 30min, gently rotates water body clockwise with glass bar, to the water flows gently, calculating, which rests on, is answered
Swash the shrimp seedling quantity of the confirmation death among jar, and records one by one；Obtain the seedling field workshop the B pond the 3-6# resisting stress situation.Knot
Fruit is as shown in table 2.
Table 2: the workshop certain nursery B pond 3-6 resisting stress situation record
| Workshop | Chi Hao | Stage | Strain | Detection time | Seedling lake salt degree (‰) | Resisting stress survival rate |
| B | 3# | P10 | It is fast big | 2018.10.06 | 24 | 70% |
| B | 4# | P10 | It is fast big | 2018.10.06 | 24 | 90% |
| B | 5# | P10 | It is fast big | 2018.10.06 | 24 | 95% |
| B | 6# | P10 | It is fast big | 2018.10.06 | 24 | 93% |
Even if 2 result of table also indicates that shrimp seedling quality in the same workshop, the ability of resisting stress is also different, if but only from
Distinguished on sense organ, naked eyes be difficult to distinguish really, salinity of the present invention stress method provided effectively for the selection of shrimp seedling quality
With reference to.
The above embodiments and description only illustrate the principle of the present invention and most preferred embodiment, is not departing from this
Under the premise of spirit and range, various changes and improvements may be made to the invention, these changes and improvements both fall within requirement and protect
In the scope of the invention of shield.
Claims (3)
1. a kind of evaluation method of Penaeus Vannmei shrimp seedling anti-stress ability, it is characterised in that: the evaluation method includes following
Step:
Step A: detecting the water body in shrimp seedling source, and the index of detection includes salinity, water temperature and appearance oxygen；
Step B: recording the cultivation number of days of shrimp seedling, and it is 14-15 days that PL5 shrimp seedling, which cultivates number of days, and it is 19-20 that PL10 shrimp seedling, which cultivates number of days,
It；
Step C: adjust test water quality, water quality is transparent sterile, PL5 shrimp seedling stress water quality be salinity 15ppt, salinity gradient
Difference > 7ppt, total alkalinity 150-180mg/L, water temperature are 29-30 DEG C, dissolved oxygen > 5mg/L；PL10 shrimp seedling stress Water salinity be
0ppt and 30ppt, total alkalinity 150-180mg/L, 29-30 DEG C of water temperature, dissolved oxygen > 5mg/L；
Step D: test PL5 shrimp seedling resisting stress survival rate prepares the white jar of plastics of 1L capacity, and jar bore is 10cm, a height of
The test water 1L of ready PL5 shrimp seedling is added in 13cm, and taking the PL5 shrimp seedling of 100 tails health to be put into stress be in water, clockwise
Water body is rotated, shrimp seedling is made to be evenly distributed, water whirlpool center is gone through and whether has and sink to the bottom or dizzy shrimp seedling, if any dead seedling or white
Seedling need to be pressed from both sides out with tweezers, and fill the health young shrimp of respective numbers；Lid is gently put on the white jar of plastics, prevents shrimp seedling from jumping
Out, retain certain gap, it is not salable, lid is opened after timing 1h, 1h, the shrimp seedling being sticked on lid is put back in water, it is suitable
Hour hands rotate water body, after waiting waterflow stabilization, intolerant to stress dead shrimp seedling can sink to bottom center, check the dead of bottom shrimp seedling
Die quantity；
Step E: PL5 shrimp seedling resisting stress survival rate is calculated；Calculation formula are as follows: PL5 shrimp seedling resisting stress survival rate=(100- death toll
Amount)/100*100%；
Step F: test PL10 shrimp seedling resisting stress survival rate prepares the white jar of plastics of two 1L capacity, and jar bore is 10cm,
A height of 13cm, first jar water salinity are 0ppt, and pure fresh water, second jar water salinity is 30ppt, it is ensured that water quality is clean
Only, other indexs are identical as described in step C；It takes the PL10 shrimp seedling of 100 tails health to be put into first jar, rotates clockwise water
Whether body makes shrimp seedling be evenly distributed, go through water whirlpool center and have and sink to the bottom or dizzy shrimp seedling, uses if any dead seedling or Bai Miaoxu
Tweezers press from both sides out, and fill the health young shrimp of respective numbers, and timing 30 minutes；All shrimp seedlings are transferred to by dredger after 30 minutes
Whether second jar, rotates clockwise water body, shrimp seedling is made to be evenly distributed, go through water whirlpool center and sink to the bottom or dizzy
Shrimp seedling is pressed from both sides out with tweezers if any dead seedling or Bai Miaoxu, and fills the health young shrimp of respective numbers, is closed the lid timing 30 minutes；
Lid is opened after 30 minutes, the shrimp seedling being sticked on lid is put back in water, rotates clockwise water body, after waiting waterflow stabilization, no
Resistance to shrimp seedling that stress be dead can sink to bottom center, check the The dead quantity of bottom seedling；
Step G: PL10 shrimp seedling resisting stress survival rate is calculated；Calculation formula are as follows: PL10 shrimp seedling resisting stress survival rate=(100- is dead
Quantity)/100*100%；
Step H: evaluation criterion: the PL10 shrimp seedling survival rate that equal PL5 shrimp seedling survival rate waits on 90% or more, quality in quality
85% or more.
2. a kind of evaluation method of Penaeus Vannmei shrimp seedling anti-stress ability according to claim 1, it is characterised in that: step
The index normal range (NR) of detection described in rapid A is salinity 15-30ppt, 29-30 DEG C of water temperature, holds oxygen 5-8mg/L.
3. a kind of evaluation method of Penaeus Vannmei shrimp seedling anti-stress ability according to claim 1, it is characterised in that: step
Poor ± 1 DEG C of temperature gradient, poor ± 1 DEG C of the temperature gradient of PL10 shrimp seedling water temperature of PL5 shrimp seedling water temperature described in rapid C.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114342849A (en) * | 2022-01-21 | 2022-04-15 | 阳江利洋苗种繁育有限公司 | Method for evaluating development maturity of young cheek of litopenaeus vannamei |
| CN114946726A (en) * | 2022-06-29 | 2022-08-30 | 中国水产科学研究院黄海水产研究所 | Heat shock treatment method for reducing or purifying decapod iridovirus 1 in bodies of shrimps and crabs for non-treatment purpose |
| CN115968821A (en) * | 2022-12-06 | 2023-04-18 | 珠海利洋苗种繁育有限公司 | A method for testing povidone-iodine stress of Penaeus vannamei shrimp seedlings |
| CN116998437A (en) * | 2023-09-06 | 2023-11-07 | 渤海水产股份有限公司 | Salinization standard coarse method for penaeus vannamei boone suitable for high-salinity mariculture |
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