CN107751067A - A kind of mating system of all-male Macrobrachium rosenbergii - Google Patents
A kind of mating system of all-male Macrobrachium rosenbergii Download PDFInfo
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- CN107751067A CN107751067A CN201711085858.2A CN201711085858A CN107751067A CN 107751067 A CN107751067 A CN 107751067A CN 201711085858 A CN201711085858 A CN 201711085858A CN 107751067 A CN107751067 A CN 107751067A
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- 241001327110 Macrobrachium rosenbergii Species 0.000 title claims abstract description 69
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- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims description 5
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- 229960003237 betaine Drugs 0.000 claims description 5
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- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 5
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 5
- 239000013535 sea water Substances 0.000 claims description 5
- AKHNMLFCWUSKQB-UHFFFAOYSA-L sodium thiosulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=S AKHNMLFCWUSKQB-UHFFFAOYSA-L 0.000 claims description 5
- DBPWSSGDRRHUNT-UHFFFAOYSA-N 17alpha-hydroxy progesterone Natural products C1CC2=CC(=O)CCC2(C)C2C1C1CCC(C(=O)C)(O)C1(C)CC2 DBPWSSGDRRHUNT-UHFFFAOYSA-N 0.000 claims description 3
- 229950000801 hydroxyprogesterone caproate Drugs 0.000 claims description 3
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- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims 1
- 229910052760 oxygen Inorganic materials 0.000 claims 1
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- 239000011669 selenium Substances 0.000 abstract description 29
- 230000004083 survival effect Effects 0.000 abstract description 12
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 abstract description 9
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- 238000009395 breeding Methods 0.000 abstract description 5
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- 238000009360 aquaculture Methods 0.000 abstract description 2
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- DBPWSSGDRRHUNT-CEGNMAFCSA-N 17α-hydroxyprogesterone Chemical compound C1CC2=CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@@](C(=O)C)(O)[C@@]1(C)CC2 DBPWSSGDRRHUNT-CEGNMAFCSA-N 0.000 description 7
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- FYGDTMLNYKFZSV-URKRLVJHSA-N (2s,3r,4s,5s,6r)-2-[(2r,4r,5r,6s)-4,5-dihydroxy-2-(hydroxymethyl)-6-[(2r,4r,5r,6s)-4,5,6-trihydroxy-2-(hydroxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1[C@@H](CO)O[C@@H](OC2[C@H](O[C@H](O)[C@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O FYGDTMLNYKFZSV-URKRLVJHSA-N 0.000 description 1
- -1 7~9 ‰ Substances 0.000 description 1
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- 239000008280 blood Substances 0.000 description 1
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- 150000002215 flavonoids Chemical class 0.000 description 1
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- 235000013305 food Nutrition 0.000 description 1
- 235000008434 ginseng Nutrition 0.000 description 1
- 210000002149 gonad Anatomy 0.000 description 1
- 239000003163 gonadal steroid hormone Substances 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/50—Culture of aquatic animals of shellfish
- A01K61/59—Culture of aquatic animals of shellfish of crustaceans, e.g. lobsters or shrimps
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/195—Antibiotics
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Marine Sciences & Fisheries (AREA)
- Environmental Sciences (AREA)
- Insects & Arthropods (AREA)
- Birds (AREA)
- Biodiversity & Conservation Biology (AREA)
- Fodder In General (AREA)
- Feed For Specific Animals (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The present invention relates to technical field of aquaculture, more particularly to a kind of mating system of all-male Macrobrachium rosenbergii.Mating system of the present invention launches different feeds according to the growth demand in Macrobrachium rosenbergii different reproductive stage, promotes the maturation of the false female shrimp of Macrobrachium rosenbergii parent, effectively shortens the breeding cycle；The survival rate of all-male Macrobrachium rosenbergii seedling is improved simultaneously, realizes a large amount of acquisitions of all-male shrimp seedling；In addition the content of selenium in shrimp is also improved, improves the nutritional quality of Macrobrachium rosenbergii.
Description
Technical field
The present invention relates to technical field of aquaculture, more particularly to a kind of mating system of all-male Macrobrachium rosenbergii.
Background technology
Macrobrachium rosenbergii (Macrobrachium rosenbergii), also known as the long-armed prawn of fresh water, Malaysian prawn, it is former
Originate in the Indian Ocean-Pacific torrid zone, subtropical water, be one of fresh water shrimp that build is maximum in the world, have growth it is fast,
It is individual big, feeding habits are wide, easily raise and train, strong adaptability, it is with short production cycle the advantages that.The method of domestic tradition cultivation Macrobrachium rosenbergii is handle
Male and female shrimp seedling is raised together together, and typically after seedling is thrown 2~3 months, female shrimp begins to sexal maturity oogenesis, now typically just no longer puts on flesh
, the nutrition eaten will be assigned on ovum.Although male Macrobrachium rosenbergii will not oogenesis, it is individual know from experience it is bigger than female shrimp, male and female is mixed
When supporting, male shrimp, which carries out mating, can consume nutrition, by a definite limitation in the speed of growth and individual specification.And all-male Roche
The problem of mating and nutrition leak is not present in pond crayfish, maintains the advantage of unisexuality, eats how much meat just can be grown for how many material, therefore in property
The advantage that growth is fast, individual is big can be fully demonstrated after maturation.
The sex of Macrobrachium rosenbergii, the male sex hormone of early stage male shrimp seedling by microsurgery, can be controlled to obtain completely
To transformation, the success of this transfer operation, the conversion of complete sex function is generated so that male shrimp becomes new female shrimp (phenotype
For female shrimp but with male gene) and have female sexual gland.Due to two identical homologues of Macrobrachium rosenbergii band of male
(ZZ), with other lobsters to possess some special knowledge as shrimps, the public shrimp after turning property, energy output 100% is male offspring.But
It is difficult often disposably to transplant sexual gland totally using microsurgry to be, low so as to cause to obtain false female shrimp quantity.
Chinese patent application (CN105647973A) provides a kind of regulation and control Macrobrachium rosenbergii male and female method for distinguishing, is in Roche
Before pond crayfish embryonic development period to the postlarva phase of postembryonal development, sequence is SEQID in reduction or silence Macrobrachium rosenbergii body
NO:1 sex albumen or the expression quantity of its derived protein are completed.By RNA perturbation techniques and gene expression regulation technology,
Sexual control is oriented, shrimps Animal Sex can be induced to reverse, but the problem of inductivity is low be present.
The content of the invention
To solve the above problems, the invention provides a kind of mating system of all-male Macrobrachium rosenbergii.Mating system of the present invention
Different feed is launched according to the growth demand in Macrobrachium rosenbergii different reproductive stage, promote the false female shrimp of Macrobrachium rosenbergii parent into
It is ripe, effectively shorten the breeding cycle；The survival rate of all-male Macrobrachium rosenbergii seedling is improved simultaneously, realizes the big of all-male shrimp seedling
Amount obtains；In addition the content of selenium in shrimp is also improved, improves the nutritional quality of Macrobrachium rosenbergii.
The present invention is realized by following scheme：
A kind of mating system of all-male Macrobrachium rosenbergii, comprises the following steps：
S1. the false female shrimp that seed selection obtains is placed under conditions of temperature is 26~28 DEG C and raises and feed feed I, raised
14-20 days to gonadal maturation, with it is physical strong, appendage is complete, gonadal maturation male shrimp carries out the fertilization that mates；
S2. after the completion for the treatment of the mating fertilization of above-mentioned steps S1 male and female shrimp, the female shrimp of oogenesis is placed in the bar that temperature is 28~30 DEG C
Under part, launch Feed II 16~20 days colors to the female shrimp belly embryo of oogenesis of raising and switch to light grey or grey by faint yellow
When, during embryonic development to zoea larva, inject seawater, 7~9 ‰, water temperature is controlled at 29~32 DEG C for salinity control；
S3. larval culture is moved to after all-male Macrobrachium rosenbergii Magna shapes nauplius in above-mentioned steps S2 cultivates 7~11 days
In groove, 1.2~1.6%, water temperature control launches feed after 28~32 DEG C, three days for larval culture trough inner water salinity control
III；
S4. after Macrobrachium rosenbergii larvae in above-mentioned steps S3 is cultivated 20~22 days, desalt processing is carried out, and move to nursery pond
Interior, water temperature is controlled at 26~28 DEG C, launches feed IV.
Preferably, feed I is made up of following component and its parts by weight in the step S1：20~40 parts of soy meal, shrimp shell
5~10 parts of 30~50 parts of powder, 1~1.5 part of glycine betaine, 1~5 part of magnesium sulfate, 5~10 parts of Se-enriched yeast and additive.
Preferably, the additive is by scutellaria glycosides, 17 α-and hydroxyprogesterone and serotonin are by weight 3:2:1 composition.
Preferably, Feed II is made up of following component and its parts by weight in the step S2：20~30 parts of fish meal, bone meal 15
~25 parts, 10~12 parts of dregs of beans, 10~15 parts of 5~8 parts of manna oligosacchride and Se-rich wheat powder.
Preferably, in the step S3 Feed II I by fairy shrimp, egg custard and papain by weight 3:2:1 group
Into.
Preferably, the step S4 nursery ponds reclaimed water is handled by aquatic products ozone cultivating system：First by water by containing sea
The micro-filter of tender activated carbon is filtered, and then carries out husky filter, is directly discharged into by valve 1/3 volume of water after sand filter mixed
Pond is closed, remaining 2/3 volume of water carries out ozone processing by ozone generator, and the water handled by ozone is discharged into mixing by valve
The water that mixing pit is directly discharged into after Chi Yusha filters carries out being mixed and added into 5~8ppm EDTA and 10~15ppm Na2S2O3, mixing
Water afterwards carries out 6~8h of aeration into aeration tank, and the water after aeration is given birth to by the biofilter containing nitrobacteria again
Change filtering, be discharged into educate by biochemistry filtered water and breed pond；First three day after all-male Macrobrachium rosenbergii larvae is launched not carry out water
In nursery pond 1/5~2/5 water is carried out above-mentioned processing by processing, launch Macrobrachium rosenbergii larvae the 4th day daily.
Preferably, feed IV is made up of following component and its parts by weight described in step S4：20~40 parts of shrimp med, fairy shrimp 10
~30 parts, 20~30 parts of Se-enriched yeast, 1~5 part of ferment powder and ginsengenin 20 .1~0.5 part.
Preferably, feed IV is made up of following component and its parts by weight described in step S4：35 parts of shrimp med, 35 parts of fairy shrimp,
25 parts of Se-enriched yeast, 4.5 parts of ferment powder and ginsengenin 20 .5 parts.
The seed selection of the false female shrimp of Macrobrachium rosenbergii parent of the present invention is according to Chinese patent application (CN105647973A) Roche natural pond
The male and female sex phenotype method of shrimp obtains.
The present invention with the addition of scutellaria glycosides, 17 α in feed I-hydroxyprogesterone and serotonin.It is scutellaria bitter, cold in nature, have
Suppress bacterium and strengthen the effect of body's immunity, scutellaria glycosides is that a kind of flavonoids is extracted to obtain from labiate scutellaria
Compound, it is one of active ingredient of scutellaria, there is the multiple pharmacological effects such as antibacterial, anti-inflammatory, due to its low, low stain and is not easy
The advantages that producing drug resistance, applied in the disease prevention and cure of multiple aquatic animal.Research has shown that 17 α-hydroxyprogesterone to prawn or
The gonad cell development and yolk accumulation of other crustaceans have obvious facilitation.Serotonin, also known as thrombocytin, ginseng
With the multinomial physiology course such as the migration of crustacean pigment, reproduction activity, husking activity, Skeletal Muscle Contraction and blood sugar concentration.This hair
Bright technical staff has found that feed I of the present invention can promote the maturation of the false female shrimp of parent, shorten the breeding cycle.
In Feed II of the present invention based on fish meal, bone meal, dregs of beans and Se-rich wheat powder, meeting the nutrition of the female shrimp of oogenesis needs
Ask；Also contain manna oligosacchride in Feed II of the present invention, manna oligosacchride can improve the female shrimp of oogenesis to thick protein, crude fat, thick
The digestion power of fiber, so as to improve the utilization rate of Feed II.
Feed II I of the present invention is by fairy shrimp, egg custard and papain by weight 3:2:1 composition.
By shrimp med, fairy shrimp, Se-enriched yeast, ferment powder and ginsenoside composition in feed IV.It can not only be improved entirely
The survival rate of male shrimp seedling, and the content that can also improve selenium in shrimp is had been surprisingly found that, improve the nutriment of Macrobrachium rosenbergii
Matter.
In addition, the present invention effectively prevents microorganism in water, virus to using water aquatic products ozone cultivating system to handle in fry pond
Deng the infringement to seedling.
The present invention has following technical advantage compared with prior art：
(1) mating system of the present invention can effectively facilitate the maturation of the false female shrimp of parent, shorten the breeding cycle；
(2) the all-male shrimp survival rate that mating system of the present invention obtains is high, realizes a large amount of acquisitions of all-male shrimp seedling, and
And the content of selenium in shrimp is also improved, improve the nutritional quality of Macrobrachium rosenbergii.
Embodiment
The present invention is further described in detail below in conjunction with embodiment.It is pointed out that following explanation is only pair
Claimed is technical scheme for example, not to any restrictions of these technical schemes.The protection of the present invention
Scope is defined by the content that appended claims are recorded.
Serotonin is purchased from Shanghai one and grinds bio tech ltd；Se-enriched yeast, soy meal are purchased from Zhengzhou Hua Feng food
Science and Technology Ltd.；Shrimp med is purchased from the emerging hundred million marine biotechnologies limited company in Guangdong；Bone meal is purchased from the grand brightness biology in Shijiazhuang
Science and Technology Ltd.；Ginsenoside is purchased from the grand bio tech ltd of Hubei good luck；17 α-hydroxyprogesterone is purchased from Hubei Ju Sheng sections
Skill Co., Ltd；Se-rich wheat powder is purchased from Shandong Jinsui Agricultural Development Co., Ltd..
A kind of mating system of all-male Macrobrachium rosenbergii of embodiment 1
A kind of mating system of all-male Macrobrachium rosenbergii, comprises the following steps：
S1. the false female shrimp that seed selection obtains is placed under conditions of temperature is 28 DEG C and raises and feed feed I, raising 14~20
It to gonadal maturation, with it is physical strong, appendage is complete, gonadal maturation male shrimp carries out the fertilization that mates；The feed I
It is made up of following component and its parts by weight：25 parts of soy meal, 40 parts of shrimp shell meal, 1.2 parts of glycine betaine, 3.6 parts of magnesium sulfate, selenium-rich ferment
Female 7.5 parts and 6 parts of additive；Additive is by scutellaria glycosides, 17 α in the feed I-and hydroxyprogesterone and serotonin are by weight 3:
2:1 composition；
S2. after the completion for the treatment of the mating fertilization of above-mentioned steps S1 male and female shrimp, the female shrimp of oogenesis is placed in temperature as under conditions of 30 DEG C,
Launch Feed II raise 16~20 days colors to the female shrimp belly embryo of oogenesis by it is faint yellow switch to light grey or grey when, embryo
When being developed to zoea larva, seawater is injected, 8.5 ‰, water temperature is controlled at 30 DEG C for salinity control；The Feed II is by following component
And its parts by weight composition：13 parts of 25 parts of fish meal, 20 parts of bone meal, 10 parts of dregs of beans, 7 parts of manna oligosacchride and Se-rich wheat powder；
S3. larval culture is moved to after all-male Macrobrachium rosenbergii Magna shapes nauplius in above-mentioned steps S2 cultivates 7~11 days
In groove, 1.4%, Feed II I is launched in water temperature control after 30 DEG C, three days for larval culture trough inner water salinity control；It is described
Feed II I is by fairy shrimp, egg custard and papain by weight 3:2:1 composition；
S4. after Macrobrachium rosenbergii larvae in above-mentioned steps S3 is cultivated 20-22 days, desalt processing is carried out, and move to nursery pond
Interior, water temperature is controlled at 28 DEG C, launches feed IV；The feed IV is made up of following component and its parts by weight：35 parts of shrimp med, good year
35 parts of worm, 25 parts of Se-enriched yeast, 4.5 parts of ferment powder and ginsengenin 20 .5 parts.
Nursery pond reclaimed water is handled by aquatic products ozone cultivating system：Water is first passed through into the microfiltration containing sponge and activated carbon
Device is filtered, and then carries out husky filter, and 1/3 volume of water is directly discharged into mixing pit, remaining 2/3 volume of water by valve after sand filter
Ozone processing is carried out by ozone generator, the water by ozone processing is discharged into after mixing pit is filtered with sand by valve and is directly discharged into
The water of mixing pit carries out being mixed and added into 7ppm EDTA and 12ppm Na2S2O3, mixed water is aerated into aeration tank
6h, the water after aeration carry out biochemistry filtered by the biofilter containing nitrobacteria again, are discharged into by biochemistry filtered water
Educate and breed pond；Launch first three day after all-male Macrobrachium rosenbergii larvae not handle water, launch the 4th of Macrobrachium rosenbergii larvae
In nursery pond 1/5 water is carried out above-mentioned processing by it daily.
A kind of mating system of all-male Macrobrachium rosenbergii of embodiment 2
A kind of mating system of all-male Macrobrachium rosenbergii, comprises the following steps：
S1. the false female shrimp that seed selection obtains is placed under conditions of temperature is 26 DEG C and raises and feed feed I, raising 14~20
It to gonadal maturation, with it is physical strong, appendage is complete, gonadal maturation male shrimp carries out the fertilization that mates；The feed I
It is made up of following component and its parts by weight：20 parts of soy meal, 30 parts of shrimp shell meal, 1 part of glycine betaine, 1 part of magnesium sulfate, Se-enriched yeast 5
5 parts of part and additive；Additive is by scutellaria glycosides, 17 α in the feed I-and hydroxyprogesterone and serotonin are by weight 3:2:1 group
Into；
S2. after the completion for the treatment of the mating fertilization of above-mentioned steps S1 male and female shrimp, the female shrimp of oogenesis is placed in temperature as under conditions of 30 DEG C,
Launch Feed II raise 16~20 days colors to the female shrimp belly embryo of oogenesis by it is faint yellow switch to light grey or grey when, embryo
When being developed to zoea larva, seawater is injected, 7 ‰, water temperature is controlled at 29 DEG C for salinity control；The Feed II by following component and
Its parts by weight forms：10 parts of 20 parts of fish meal, 15 parts of bone meal, 10 parts of dregs of beans, 5 parts of manna oligosacchride and Se-rich wheat powder；
S3. larval culture is moved to after all-male Macrobrachium rosenbergii Magna shapes nauplius in above-mentioned steps S2 cultivates 7~11 days
In groove, 1.2%, Feed II I is launched in water temperature control after 28 DEG C, three days for larval culture trough inner water salinity control；It is described
Feed II I is by fairy shrimp, egg custard and papain by weight 3:2:1 composition；
S4. after Macrobrachium rosenbergii larvae in above-mentioned steps S3 is cultivated 20~22 days, desalt processing is carried out, and move to nursery pond
Interior, water temperature is controlled at 26 DEG C, launches feed IV；The feed IV is made up of following component and its parts by weight：20 parts of shrimp med, good year
10 parts of worm, 20 parts of Se-enriched yeast, 1 part of ferment powder and ginsengenin 20 .1 parts.
Nursery pond reclaimed water is handled by aquatic products ozone cultivating system：Water is first passed through into the microfiltration containing sponge and activated carbon
Device is filtered, and then carries out husky filter, and 1/3 volume of water is directly discharged into mixing pit, remaining 2/3 volume of water by valve after sand filter
Ozone processing is carried out by ozone generator, the water by ozone processing is discharged into after mixing pit is filtered with sand by valve and is directly discharged into
The water of mixing pit carries out being mixed and added into 5ppm EDTA and 10ppm Na2S2O3, mixed water is aerated into aeration tank
6-8h, the water after aeration carry out biochemistry filtered by the biofilter containing nitrobacteria again, are arranged by biochemistry filtered water
Enter to educate and breed pond；Launch first three day after all-male Macrobrachium rosenbergii larvae not handle water, launch the of Macrobrachium rosenbergii larvae
In nursery pond 2/5 water is subjected to above-mentioned processing daily within four days.
A kind of mating system of all-male Macrobrachium rosenbergii of embodiment 3
A kind of mating system of all-male Macrobrachium rosenbergii, comprises the following steps：
S1. the false female shrimp that seed selection obtains is placed under conditions of temperature is 28 DEG C and raises and launch feed I, raising 14~20
It to gonadal maturation, with it is physical strong, appendage is complete, gonadal maturation male shrimp carries out the fertilization that mates；The feed I
It is made up of following component and its parts by weight：40 parts of soy meal, 50 parts of shrimp shell meal, 1.5 parts of glycine betaine, 5 parts of magnesium sulfate, Se-enriched yeast
10 parts and 10 parts of additive；Additive is by scutellaria glycosides, 17 α in the feed I-and hydroxyprogesterone and serotonin are by weight 3:2:1
Composition；
S2. after the completion for the treatment of the mating fertilization of above-mentioned steps S1 male and female shrimp, the female shrimp of oogenesis is placed in temperature as under conditions of 28 DEG C,
Launch Feed II raise 16~20 days colors to the female shrimp belly embryo of oogenesis by it is faint yellow switch to light grey or grey when, embryo
When being developed to zoea larva, seawater is injected, 9 ‰, water temperature is controlled at 32 DEG C for salinity control；The Feed II by following component and
Its parts by weight forms：15 parts of 30 parts of fish meal, 25 parts of bone meal, 12 parts of dregs of beans, 8 parts of manna oligosacchride and Se-rich wheat powder；
S3. larval culture is moved to after all-male Macrobrachium rosenbergii Magna shapes nauplius in above-mentioned steps S2 cultivates 7~11 days
In groove, 1.6%, Feed II I is launched in water temperature control after 32 DEG C, three days for larval culture trough inner water salinity control；It is described
Feed II I is by fairy shrimp, egg custard and papain by weight 3:2:1 composition；
S4. after Macrobrachium rosenbergii larvae in above-mentioned steps S3 is cultivated 20~22 days, desalt processing is carried out, and move to nursery pond
Interior, water temperature is controlled at 28 DEG C, launches feed IV；The feed IV is made up of following component and its parts by weight：40 parts of shrimp med, good year
30 parts of worm, 30 parts of Se-enriched yeast, 5 parts of ferment powder and ginsengenin 20 .5 parts.
Nursery pond reclaimed water is handled by aquatic products ozone cultivating system：Water is first passed through into the microfiltration containing sponge and activated carbon
Device is filtered, and then carries out husky filter, and 1/3 volume of water is directly discharged into mixing pit, remaining 2/3 volume of water by valve after sand filter
Ozone processing is carried out by ozone generator, the water by ozone processing is discharged into after mixing pit is filtered with sand by valve and is directly discharged into
The water of mixing pit carries out being mixed and added into 8ppm EDTA and 15ppm Na2S2O3, mixed water is aerated into aeration tank
8h, the water after aeration carry out biochemistry filtered by the biofilter containing nitrobacteria again, are discharged into by biochemistry filtered water
Educate and breed pond；Launch first three day after all-male Macrobrachium rosenbergii larvae not handle water, launch the 4th of Macrobrachium rosenbergii larvae
In nursery pond 1/5 water is carried out above-mentioned processing by it daily.
A kind of mating system of all-male Macrobrachium rosenbergii of comparative example 1
Difference with embodiment 1 is free of additive in the feed I launched in step S1, other steps with implementation
Example 1 is similar.
A kind of mating system of all-male Macrobrachium rosenbergii of comparative example 2
Difference with embodiment 1 replaces manna oligosacchride in the Feed II launched in step S2 with beta glucan, other
Step is similar to Example 1.
A kind of mating system of all-male Macrobrachium rosenbergii of comparative example 3
Difference with embodiment 1 replaces ginsenoside in the feed IV launched in the step S4 with antibacterial peptide, its
Its step is similar to Example 1.
The measure of the false female shrimp maturing rate of the Macrobrachium rosenbergii parent of test example 1
The false female shrimp of the consistent Macrobrachium rosenbergii parent of the development of ovary that seed selection is obtained, by embodiment 1~3 and the institute of comparative example 1
State mating system to carry out after cultivating 14 days, false female shrimp sexual gland is observed, and calculate ovary maturity rate, be i.e. the development of ovary is in
Ratio shared by the close shrimp of IV phase, as a result as shown in table 1.
The false female shrimp maturing rate of 1 different groups of table
| Group | Conversion ratio (%) |
| Embodiment 1 | 98.5% |
| Embodiment 2 | 96.0% |
| Embodiment 3 | 95.3% |
| Comparative example 1 | 73.0% |
As shown in Table 1, in embodiment 1~3 the false female shrimp ovary maturity rate of Macrobrachium rosenbergii parent apparently higher than comparative example.Its
In, compared with comparative example 1, the maturing rate of embodiment 1 improves 34.9%, illustrates scutellaria glycosides, 17 α-and hydroxyprogesterone and serotonin are matched somebody with somebody
The false female shrimp ovary maturity of parent can be effectively facilitated by closing use, so as to effectively shorten the breeding cycle.
The all-male shrimp survival rate of seedling of test example 2
The all-male shrimp seedling obtained by embodiment 1~3 and the mating system of comparative example 2 is cultivated, and presses following public affairs
Formula carries out survival rate measure, as a result as shown in table 2.
Survival rate=(total juvenile prawn number-dead juvenile prawn number)/total juvenile prawn number × 100%
The all-male Macrobrachium rosenbergii survival rate of seedling of table 2
| Group | Survival rate (%) |
| Embodiment 1 | 93.0% |
| Embodiment 2 | 91.2% |
| Embodiment 3 | 92.6% |
| Comparative example 2 | 83.4% |
| Comparative example 3 | 88.4% |
As shown in Table 2, the survival rate for the all-male Macrobrachium rosenbergii seedling that the mating system of the embodiment of the present invention 1~3 obtains is equal
More than 90%.Compared with comparative example 2, the survival rate of embodiment 1 improves 11.5%, illustrates that the addition of manna oligosacchride is more conducive to all-male
Utilization of the property Macrobrachium rosenbergii seedling to Feed II, so as to improve the survival rate of seedling.
Determination of Selenium in the all-male shrimp of test example 3
Respectively by embodiment 1~3 and the mating system of comparative example 3 by after the all-male shrimp seedling culture 90 days of acquisition, detection
The content of every group of shrimp selenium, specific data are as shown in table 3.
The measure of Se content in the all-male shrimp of table 3
| Group | Total Se content (μ g/kg) |
| Embodiment 2 | 907.8 |
| Embodiment 3 | 908.2 |
| Comparative example 3 | 762.9 |
As shown in Table 3, the content by selenium in the all-male shrimp of the mating system of the embodiment of the present invention 1~3 acquisition is higher, with
Comparative example 3 is compared, and total Se content improves 19.1% in 1 male shrimp of embodiment, and illustrating that ginsenoside coordinates with Se-enriched yeast makes
With the content that can significantly improve selenium in all-male shrimp.
Above-described is only some embodiments of the present invention, but embodiments of the present invention are not by above-described embodiment
Limitation.For the person of ordinary skill of the art, under other any Spirit Essences and principle without departing from the present invention
Change, modification, replacement, the simplification made, should be equivalent substitute mode, are included within protection scope of the present invention.
Claims (8)
1. a kind of mating system of all-male Macrobrachium rosenbergii, it is characterised in that comprise the following steps：
S1. the false female shrimp of Macrobrachium rosenbergii parent seed selection obtained, which is placed under conditions of temperature is 26~28 DEG C, to be raised and feeds feed
I, raising 14~20 days to its gonadal maturation, with it is physical strong, appendage is complete, gonadal maturation male shrimp is mated
Fertilization；
S2. after the completion for the treatment of the mating fertilization of above-mentioned steps S1 male and female shrimp, the female shrimp of oogenesis is placed in temperature as under conditions of 28~30 DEG C,
Launch Feed II raise 16~20 days colors to the female shrimp belly embryo of oogenesis by it is faint yellow switch to light grey or grey when, embryo
When being developed to zoea larva, seawater is injected, 7~9 ‰, water temperature is controlled at 29~32 DEG C for salinity control；
S3. young training is moved to after the all-male Macrobrachium rosenbergii Magna shapes nauplius obtained in above-mentioned steps S2 cultivates 7~11 days
Educate in groove, 1.2~1.6%, water temperature control is launched after 28~32 DEG C, three days raises for larval culture trough inner water salinity control
Expect III；
S4. after all-male Macrobrachium rosenbergii larvae in above-mentioned steps S3 is cultivated 20~22 days, desalt processing is carried out, and move to nursery pond
Interior, water temperature is controlled at 26~28 DEG C, launches feed IV.
2. the mating system of all-male Macrobrachium rosenbergii according to claim 1, it is characterised in that in the step S1 feed I by
Following component and its parts by weight composition：20~40 parts of soy meal, 30~50 parts of shrimp shell meal, 1~1.5 part of glycine betaine, magnesium sulfate 1~
5 parts, 5~10 parts of 5~10 parts of Se-enriched yeast and additive.
3. the mating system of all-male Macrobrachium rosenbergii according to claim 2, it is characterised in that the additive by scutellaria glycosides,
17 α-hydroxyprogesterone and serotonin are by weight 3:2:1 composition.
4. the mating system of all-male Macrobrachium rosenbergii according to claim 1, it is characterised in that in the step S2 Feed II by
Following component and its parts by weight composition：20~30 parts of fish meal, 15~25 parts of bone meal, 10~12 parts of dregs of beans, 5~8 parts of manna oligosacchride
With 10~15 parts of Se-rich wheat powder.
5. the mating system of all-male Macrobrachium rosenbergii according to claim 1, it is characterised in that Feed II I in the step S3
By fairy shrimp, egg custard and papain by weight 3:2:1 composition.
6. according to the mating system of all-male Macrobrachium rosenbergii described in right 1, it is characterised in that the step S4 nursery ponds reclaimed water is passed through
The processing of aquatic products ozone cultivating system：First water is filtered by the micro-filter containing sponge and activated carbon, then carried out husky
Filter, 1/3 volume of water is directly discharged into mixing pit by valve after sand filter, remaining 2/3 volume of water carries out smelly by ozone generator
Oxygen processing, the water by ozone processing are discharged into mixing pit by valve and mixed simultaneously with being directly discharged into the water of mixing pit after husky filter
Add 5~8ppm EDTA and 10~15ppm Na2S2O3, mixed water carries out 6~8h of aeration into aeration tank, after aeration
Water is biochemistry filtered by the biofilter progress containing nitrobacteria again, is discharged into educate by biochemistry filtered water and breeds pond；Throw
Put first three day after all-male Macrobrachium rosenbergii larvae not handle water, launch the 4th day of Macrobrachium rosenbergii larvae daily by nursery
1/5~2/5 water carries out above-mentioned processing in pond.
7. the mating system of all-male Macrobrachium rosenbergii according to claim 1, it is characterised in that feed IV described in step S4 by with
Lower composition and its parts by weight composition：20~40 parts of shrimp med, 10~30 parts of fairy shrimp, 20~30 parts of Se-enriched yeast, ferment powder 1~5
Part and ginsengenin 20 .1~0.5 part.
8. the mating system of all-male Macrobrachium rosenbergii according to claim 1, it is characterised in that feed IV described in step S4 by with
Lower composition and its parts by weight composition：35 parts of shrimp med, 35 parts of fairy shrimp, 25 parts of Se-enriched yeast, 4.5 parts of ferment powder and ginsenoside
0.5 part.
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| CN108770742A (en) * | 2018-05-28 | 2018-11-09 | 苏州市毛氏阳澄湖水产发展有限公司 | A kind of method for culturing seedlings of Macrobrachium rosenbergii high-survival rate |
| CN109392789A (en) * | 2018-12-11 | 2019-03-01 | 佛山科学技术学院 | A kind of method that thermal stimulus influences Macrobrachium rosenbergii Sex Differentiation |
| CN109601451A (en) * | 2019-01-30 | 2019-04-12 | 盐城工学院 | A method of improving Macrobrachium rosenbergii egg laying rate of red swamp |
| CN111387105A (en) * | 2020-03-12 | 2020-07-10 | 浙江省淡水水产研究所 | Method for producing seeds of all-male macrobrachium rosenbergii |
| CN112841096A (en) * | 2020-12-31 | 2021-05-28 | 浙江省淡水水产研究所 | Breeding method of all-male macrobrachium rosenbergii rapid-growth strain |
| CN114304016A (en) * | 2021-11-30 | 2022-04-12 | 江苏海洋大学 | Freshwater crayfish breeding method |
| CN115067239A (en) * | 2022-07-06 | 2022-09-20 | 泉州市嗣家名茶发展有限公司 | Limulus gigas rapid fattening breeding method for blood extraction |
| CN116349629A (en) * | 2023-04-25 | 2023-06-30 | 浙江省淡水水产研究所 | Indoor intensified cultivation method for macrobrachium rosenbergii |
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| CN109392789A (en) * | 2018-12-11 | 2019-03-01 | 佛山科学技术学院 | A kind of method that thermal stimulus influences Macrobrachium rosenbergii Sex Differentiation |
| CN109601451A (en) * | 2019-01-30 | 2019-04-12 | 盐城工学院 | A method of improving Macrobrachium rosenbergii egg laying rate of red swamp |
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| CN111387105B (en) * | 2020-03-12 | 2022-02-15 | 浙江省淡水水产研究所 | Method for producing seeds of all-male macrobrachium rosenbergii |
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| CN112841096B (en) * | 2020-12-31 | 2022-08-05 | 浙江省淡水水产研究所 | Breeding method of all-male macrobrachium rosenbergii rapid-growth strain |
| CN114304016A (en) * | 2021-11-30 | 2022-04-12 | 江苏海洋大学 | Freshwater crayfish breeding method |
| CN115067239A (en) * | 2022-07-06 | 2022-09-20 | 泉州市嗣家名茶发展有限公司 | Limulus gigas rapid fattening breeding method for blood extraction |
| CN115067239B (en) * | 2022-07-06 | 2023-03-10 | 泉州市嗣家名茶发展有限公司 | Limulus gigas rapid fattening breeding method for blood extraction |
| CN116349629A (en) * | 2023-04-25 | 2023-06-30 | 浙江省淡水水产研究所 | Indoor intensified cultivation method for macrobrachium rosenbergii |
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