KR20130041464A - Seedling production technique in early out-of-season for korean bullhead - Google Patents

Abstract

PURPOSE: A bullhead early seeding production method is provided to provide a base for an early seeding production and a complete culture through an efficient maturation induction method of a bullhead brood fish. CONSTITUTION: A bullhead early seeding production method comprises following steps: a step of collecting a bullhead brood fish; a step of maintaining the brood fish in a constant breeding water temperature, domesticate and culture for two weeks; a step of maturing the cultured brood fish by moving to a circulation filtering type brood fish water tub and controlling the water temperature and the photo period. The constant breeding water temperature is 23>=. For the stabilization and feed acclimation of the brood fish, an external shock is excluded and a small amount of the freeze small prawn is supplied as a main feeding twice a day to domesticate. The bullhead is cultured for a three-month early mature based on the photo period and the water temperature condition of mid-June which is the spawning season of the cultured bullhead. [Reference numerals] (AA) Bullhead adult fish production; (BB) Bullhead mother collection; (CC) Management and breeding; (DD) Sexual maturity inducement by environment control; (EE) Spawning and fertilization; (FF) Roe growing; (GG) Bullhead roe production; (HH) Bullhead farming;

Description

SEEDLING PRODUCTION TECHNIQUE IN EARLY OUT-OF-SEASON FOR KOREAN BULLHEAD}

The present invention relates to a method for the production of early seedlings, which induces the management and early maturation of larva larvae, in particular using wild and cultured larvae, inducing premature maturation through regulation of water temperature and photoperiod and It is related to the method of producing seedlings in early age to secure seedlings and produce seedlings 3-4 months earlier than the natural spawning season.

Dongjadae is a freshwater subfamily belonging to the catfish tree, and lives in the sandy and mud bottoms of the lower streams. It is better known to the fisherman in the dialect of Pagasari.

In other words, the catfish is a catfish and looks similar to a catfish, but the body is smaller than the catfish, and the body color, fin shape, and number of mustaches are different. Overall, the body is long and the head is flat up and down, and toward the back of the body flattened to the side. The snout is sharp and flat and the upper jaw is longer than the lower jaw. The mouth is torn to the side and there are four pairs of beards around the mouth. The caudal fin deeply splits, and the pectoral fin has strong spines with serrations. Next to the body passes three large rectangular patterns with a dark brown color.

They are nocturnal, living in the middle and downstream of large rivers with slow water flows, near the sand and mud bottoms of lakes, and hiding between stone clefts during the day and mainly seeking food at night. They live on insects, crustaceans, young fish or fish eggs that live in the water. When mating begins in early summer, the male digs out the mud with the thorns of the hard pectoral fin, creating a place for the female to lay eggs and await the female. When a female chooses a suitable place for laying eggs, the male who prepares the site fertilizes the egg and protects the eggs and pups until the pups grow and become independent. The offspring, which are about 2.6 cm long, resemble those of mature adults. After two to three years, the young are fully grown.

On the other hand, Dongjagae is a high-quality fish traded at the highest price (12,000 ~ 17,000 won / kg) among catfish families.

Aquaculture production of 50 tons in 2000, 128 tons in 2010, fishery production in 59 tons in 2000 and 263 tons in 2010 is a major industry in the inner water surface, and there are many demands for aquaculture and fishery resources. .

In order to produce the seedlings of the cauliflower, it is not possible to ship them because of the short period of aquaculture, since they use the catched fish (mature fish stocked or held for breeding) during natural spawning. Accordingly, due to the concentrated demand of wild fish, there are problems such as a 4 times increase in the cost of purchasing fish, the planned supply of seedlings is impossible due to inadequate supply and demand of fish.

Therefore, if the seedling is produced by shortening the natural spawning period by three to four months, the shipment will be possible. In addition, maturation induction techniques can be used to provide a base for early seedling production and complete farming.

On the other hand, the following patent document discloses techniques for nurturing parentfish or inducing early spawning.

In other words, Patent Document 1 discloses a technique for controlling water temperature, photoperiod and salinity concentration for maturation control at the stage of rearing larvae of scorpion. In the method of production, a female is treated with a gonadotropin-releasing hormone analog or a lutein-stimulating hormone-releasing hormone analog and a male is treated with a human placental gonadotropin or pituitary extract of a fish which is a gonad-stimulating hormone analog. At 9-23 ° C, the photoperiod has been disclosed for the production of fertilized egg from perch to mature mature fish with mutual control within the range of 0 to 3800 lux and day length of 7-14 hours.

In addition, Patent Document 3 discloses aquaculture methods for solving water pollution by using microbiological circulating filtration method in aquaculture farms in which high-density rolls of fish are farmed, and in Patent Document 4 below, induction of early spawning of horsefish and Seedling production method is disclosed.

Republic of Korea Patent Publication No. 2008-0020810 (2008.03.06 published) Republic of Korea Patent Publication No. 1999-0045853 (published on June 25, 1999) Republic of Korea Patent Publication No. 1997-0025382 (Published June 24, 1997) Republic of Korea Patent Publication No. 2005-0102458 (published Oct. 26, 2005)

However, in the conventional technique as described above, there is no disclosure about the early seedling production of the same.

An object of the present invention has been made to solve the problems described above, and to prepare a base for the complete culture of the dongjagi and the maturing induction of wild and aquaculture herds to produce seedlings 3-4 months earlier than the natural spawning season It is to provide a method for producing early breeders of dogs.

In order to achieve the above object, the method for producing early seedlings of a donkey in accordance with the present invention is a method of inducing maturation of fish for early seedling production, (a) collecting the mother-daughter, (b) step (a) Maintaining the larvae at a constant breeding water temperature of the two weeks of pure and breeding, (c) comprising the step of transferring the larvae reared in the step (c) to the circulating filtration fish tank and maturing by adjusting the water temperature and photoperiod It features.

In addition, in the method for producing a seedling early age seedling according to the invention, the constant breeding water temperature is characterized in that 23 ℃.

In addition, in the method for producing a seedling of early winter seedlings according to the present invention, the step (b) excludes external shock for stabilization of the larvae and food preservation, and supplies frozen small shrimp twice a day as a main food to incubate. It is characterized by.

In the method of producing early seedlings in accordance with the present invention, the dogs are bred for early maturation of three months on the basis of photoperiod and water temperature conditions in mid-June as spawning, the photoperiod is fixed for 12 hours , The water temperature conditions are characterized by maintaining after rising from 23 ℃ to 23 ℃ sequentially for two months.

In addition, in the method for producing early seedlings of the cauliflower dog according to the present invention, the cauliflower dog is bred for premature maturation for three months based on the photoperiod and water temperature conditions in mid-June, which are spawning seasons, either wild or farmed. It is sequentially raised from 12 to 15 hours, the water temperature conditions are characterized in that after maintaining for 1 month after sequentially rising from 15 ℃ to 23 ℃.

In addition, in the method for producing early seedlings of the cauliflower, according to the present invention, the spawning induction of the cauliflower dog is characterized by inducing spawning by using a spawning-inducing hormone Ovaprim and HCG in combination.

In addition, in the method of producing seedlings of the same age according to the present invention, the spawning induction of the copper dogs is HCG injection after 24 hours after the injection of Obavam, the hatching temperature is characterized in that 25 ± 0.5 ℃ .

In addition, in the method for producing early seedlings of the cauliflower in accordance with the present invention, the fertilization of the cauliflower dog is carried out by the dry method, the fertilized egg hatched at 25 ± 0.5 ℃, hatched larvae after 10 days of Artemia sp. It is characterized by the breeding using the compounded feed according to the growth stage.

As described above, according to the method for producing early seedlings of the larvae in accordance with the present invention, through the efficient maturation method of the larvae of the larvae, the effect of providing the basis for the early seed production and complete farming can be obtained.

1 is a whole process of the early seedling production method of the same age,
2 is a view showing a circulating filtration breeding tank (FRP, PVC) used in the experiment,
Figure 3 is a graph showing the experimental conditions for the early maturity of pupae in Experiment I (FRP 500L),
Figure 4 is a graph showing the water temperature and photoperiod for early maturity induction of pupae in Experiment II (PVC 200L),
5 and 6 are views showing the morphology of the ovary of wild and cultured dorsum dogs during the experiment for early maturation
7 is a graph showing the change in gonad weight index (GSI) according to the breeding water temperature and photoperiod control of cultured Dongjak dog in Experiment I,
Figure 8 is a graph showing the change in gonad weight index (GSI) according to the breeding water temperature and photoperiod control of cultured and natural pupae dog in Experiment II.

These and other objects and novel features of the present invention will become more apparent from the description of the present specification and the accompanying drawings.

Hereinafter, a method of inducing maturation of larvae for the early seedling production of cauliflower according to the present invention will be described as follows.

Early generation seedling production in accordance with the present invention goes through a process as shown in FIG. 1 is an overall process diagram of the method for producing early seedlings of the same age.

Each process according to FIG. 1 will be described sequentially.

First, the first step was to secure a mother-child language.

For the early seedling production of Dongjagae according to the present invention, natural wild fish were purchased from wholesalers of freshwater fish such as Jeonbuk Jeonju City, Namyang Bay Fishing Village, Gyeonggi-do, and Sap Gyoho, Chungnam. It was.

The full length and body weight range of wild female pupa were 14.5 ~ 20.0cm and 27.3 ~ 67.03g, respectively. The males had a full length and weight range of 15.0 ~ 25.5cm and 35.9 ~ 127. 3g, respectively.

In addition, the total length and weight of females were 27.5 ~ 19.9㎝ and 62.7 ~ 94.3g, respectively. The males were 20.0 ~ 24.0㎝ and 87.0 ~ 124.4g, respectively.

Table 1 shows the average length and weight of wild and aquaculture cauliflower dogs secured as described above.

Next, parenthesis management will be described as a second step.

The larvae of wild-caught larvae grown in wild and aquaculture farms are more difficult than other fish species, especially those with poor food purity and high mortality caused by bacterial diseases. In addition, the species is very sensitive to the external environment, and in order to stabilize the larvae and purify the food, the temperature of the water is minimized at 23 ℃, and the frozen Shrimp (Palaemon paucidens De Haan) is supplied twice a day as the main food. . Feeding reaction started to appear 7 days after the feeding of shrimp, and since then, the feeding amount has been continuously increased and vigorous feeding was started on the 14th.

It is most important to exclude the stressful environment such as breeding environment, water quality and sudden changes of illumination in order to keep the natural Pudong pup indoors. It can be sown in 7-10 days after breeding.

Next, the maturity induction was divided into experimental groups in the third step.

The design of the experimental group was largely divided into three experimental groups, and was carried out in a water tank as shown in FIG. 2, and the results are shown in FIGS. 3 and 4.

Figure 2 is a view showing the circulating filtration breeding tank (FRP, PVC) used in the experiment, Figure 3 is a graph showing the experimental conditions for the early maturation of the same age in Experiment I (FRP 500L), Figure 4 is Experiment II (PVC 200L) is a graph showing the water temperature and photoperiod for early maturity induction.

end. Experiment Ⅰ (Exp Ⅰ)

Based on the photoperiod and water temperature conditions in mid-June, the spawning season of this species, the experimental group for early maturation of three months was designed. The control group is groundwater (14 ℃) running water, water temperature (20 ℃) and natural light cycle, photoperiod fixed at 12 hours, only water temperature rise, water temperature and photoperiod breeding group (see Fig. 2 and 3, Table 2) )

Table 2 shows the experimental conditions for premature maturity induction in Experiment I.

I. Experiment Group II (Exp II)

Difference between wild and cultured fish, whether or not low-temperature experience, was tested in a 200L tank divided into experimental groups of fixed water temperature 23 ℃ (see Fig. 2 and 4, Table 3).

Table 3 shows the experimental conditions for premature maturity induction in Experiment II.

Next, scattering induction and fertilization were carried out as a fourth step.

The mating stocks were fertilized by dry method to induce spawning using a mixture of ovaprim (Ovaprim, 0.5ml / kg) and HCG (20,000 IU / kg). Fertilized eggs were hatched at 25 ± 0.5 ℃, and hatched larvae were produced for 10 days with Artemia sp.

That is, the scattering induction method was as follows.

Obavam (Ovaprim, 0.5ml / kg) and HCG (20,000IU / kg) were used to secure mature eggs from artificially induced adult and aquaculture cauliflower females. Fertilization rate and hatching rate are shown in Table 4.

As a result of hormonal concentration, no eggs were spawned in all the experiments treated with chlorine alone, whereas in the experiments in which HCG was injected 24 hours after the injection of obaprim, which was a parallel treatment, 7 out of 10 in 10 to 20 hours. Marie spawned.

At this time, fertilization rate and hatching rate were 89.7 ± 10.4% and 75.5 ± 16.5%, respectively, which were different from the mean pupillary fertilization rate (60 ~ 90%) and hatching rate (40 ~ 80%, National Fisheries Research and Development Institute report). Did not look.

Hereinafter, the present invention will be described in detail with reference to Examples, but these are not intended to limit the content of the invention.

<Example 1> Early maturity induction of paternity fish

First, the morphology of the ovary was observed.

1) Before starting experiment

As a result of observing the morphology and maturity of the female gonads of the wild and cultured pupae before the start of the experiment, the ovaries were a pair of pouches, partly adhered to the body wall, and suspended from the dorsal cavity wall by the ovarian membrane. In general, the ovarian ovary in mature stage has a large elongated cyst structure with a bure between the dorsal vertebrae and a bright yellow color.

However, they were immature compared to the individuals at maturity based on the ovarian morphology, size, and color of the wild pups used in this study. The Gonadosomatic Index (GSI) of experimental fish was 1.70 ± 0.68%, which was equivalent to mid-September to April when GSI decreased during the year.

In addition, the GSI of the female gonad of female cultured was 0.54 ± 0.10%, which was significantly lower than that of the female GSI of wild-dog. The females of cultured cauliflower dogs had an abdominal bulge, and the dissections were not mature but resulted from fat accumulation in the abdominal cavity.

Exp Exp.

As a result of anatomically observing the morphological changes of the ovary according to the experimental period, as shown in Fig. 5, the experimental group with morphological changes of the ovary at 75 days was significantly increased compared to before the start of the experiment with ExpⅠ-3, The ovary of the control group, which was flow-tested, showed little change in size.

3) Exp Ⅱ: Wild and farmed lionfish

As a result of anatomical observation of ovarian morphological changes according to the experimental period in the same maturity-inducing conditions of wild and cultured larva larvae, as shown in FIG. In Exp II-1, 2, 3, and 4 using larvae, the ovary of Exp II-3 showed clear and clear yellow eggs.

The size of the ovary was significantly increased in Exp II-6 and 8 using cultured larvae. 5 and 6 are diagrams showing the morphology of the ovary of wild and cultured dorsum dogs during the experimental period for the early maturation of pupae.

Next, the change in the gonad weight index (GSI) will be described with reference to FIGS. 7 and 8.

7 is a graph showing the change in gonad weight index (GSI) according to the breeding water temperature and photoperiod control of cultured Dongjak dog in Experiment I, Figure 8 is a gonad weight according to the breeding water temperature and photoperiod control in It is a graph showing an index (GSI) change.

1) ExpⅠ: Changes in GSI of Gonads

The GSI of the cultured female pupa was 0.54 ± 0.10% before the start of the experiment. After 30 days of experiment, ExpⅠ-1 was 1.08 ± 0.47%, ExpⅠ-2 was 0.91 ± 0.33%, and ExpⅠ-3 was 0.80 ± 0.22%. There was no significant difference.

After 60 days, the maturity values of ExpⅠ-1 were 1.53 ± 0.97%, ExpⅠ-2 was 2.10 ± 1.83%, and ExpⅠ-3 was 3.01 ± 1.97%. After 75 days, the maturity values of ExpⅠ-1 were 2.15 ± 2.47%, ExpⅠ-2 was 6.05 ± 2.94%, and ExpⅠ-3 was 5.42 ± 3.08%, which was the highest value in ExpⅠ-2.

However, the control group showed no significant change of 0.83 ± 0.27% at 30 days, 0.87 ± 0.23% at 60 days, and 0.79 ± 0.15% at 75 days (see FIG. 7).

Exp Ⅱ: Changes in GSI of Gonadal Dogs

The GSI of females in wild pups before the start of the experiment was 1.70 ± 0.68%, and after 30 days, ExpII-1 was 1.30 ± 0.32%, ExpII-2 was 1.60 ± 0.10%, ExpII-3 was 1.44 ± 0.35%, and ExpII-4 was 4.0. The highest GSI was found in Exp II-4 at ± 0.65%.

After 60 days of experiment, GSI increased rapidly with 9.61 ± 0.68% for ExpII-1, 10.51 ± 1.07 for ExpII-2, 10.71 ± 0.34% for ExpII-3 and 11.45 ± 0.40% for ExpII-4. On the 75th day of the experiment, GSI increased continuously with 14.0 ± 2.46% for ExpII-1, 13.71 ± 0.68% for ExpII-2, 11.91 ± 3.47% for ExpII-3 and 14.03 ± 0.38% for ExpII-4.

Similar trends were also observed in Exp II-5 ~ 8 test subjects in the same culture conditions. GSI was 0.54 ± 0.1% before the start of the experiment. After 30 days, ExpII-5 was 1.13 ± 0.44%, ExpII-6 was 1.05 ± 0.22%, ExpII-7 was 0.70 ± 0.21%, and ExpII-8 was 2.15 ± 1.95%. GSI was high in Exp II-8, the same environment as -4.

On the 60th day, Exp II-6 was 3.18 ± 1.16%, ExpII-7 was 3.45 ± 1.66%, ExpII-8 was 1.00 ± 0.39%, and ExpII-8 was 4.18 ± 0.59%. This lowest value was found to be Exp II-7.

On the 75th day after the experiment, the GSI value was further increased, ExpII-5 was 7.85 ± 2.22%, ExpII-6 was 8.81 ± 1.22%, ExpII-7 was 4.48 ± 2.89%, and ExpII-8 was 8.62 ± 4.04%. Showed an increased GSI value more than twice the 60 days. However, the control GSI of 0.78 ~ 0.80 did not change significantly (see Figure 8).

In Figure 8, Control represents aquaculture, Exp II-1-4 is natural, and Exp II-5-8 represents aquaculture.

Although the present invention has been described in detail with reference to the above embodiments, it is needless to say that the present invention is not limited to the above-described embodiments, and various modifications may be made without departing from the spirit of the present invention.

The present invention is applied to the production of early seedlings of the same dog.

Claims (8)

As a method of inducing maturation of parent fish for the production of early seedlings
(a) collecting the mother-son larvae,
(b) maintaining the parental fish of step (a) at a constant breeding water temperature for 2 weeks of netting and breeding,
(C) method of producing the early seedlings, characterized in that the step of transferring the larva reared in the step (c) to a circulating filtration larva tank and maturing by adjusting the water temperature and photoperiod. The method of claim 1,
The constant breeding water temperature is 23 ℃ the method of producing seedlings early. The method of claim 2,
The step (b) excludes external shocks for stabilization and food feeding of the larvae, and the method of producing early seedlings, characterized in that the frozen prawns feed a small amount twice a day as a main food. The method of claim 1,
The dongle is farmed for three months early maturation, based on photoperiod and water temperature conditions in mid-June, which are spawning seasons.
The photoperiod is fixed at 12 hours, and the water temperature conditions for 2 months to increase after the sequential rise from 15 ℃ to 23 ℃ in order to maintain early seedling production. The method of claim 1,
The Dongja dogs are bred for 3 months early maturation based on photoperiod and water temperature conditions in mid-June, which are spawning seasons, either wild or farmed.
The condition of the photoperiod is raised sequentially from 12 to 15 hours, and the water temperature conditions are maintained for 1 month after increasing sequentially from 15 ℃ to 23 ℃. The method of claim 1,
The method of induction of eggplant production, characterized in that to induce spawning using a mixture of the egg-inducing hormone Ovaprim and HCG. The method according to claim 6,
The spawning induction of the cauliflower dog is HCG injection after 24 hours after the Ovaprim injection, and hatching temperature is 25 ± 0.5 ° C The method of claim 1,
The fertilization of the same age is carried out by dry method, fertilized eggs hatch at 25 ± 0.5 ℃, hatched larvae for 10 days after breeding the Artemia sp. How to produce early childhood seedlings.

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