CN104304103B - A kind of doing sth. in advance breeds, cultivates big specification lip * offspring breeding method - Google Patents

A kind of doing sth. in advance breeds, cultivates big specification lip * offspring breeding method Download PDF

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CN104304103B
CN104304103B CN201410475021.9A CN201410475021A CN104304103B CN 104304103 B CN104304103 B CN 104304103B CN 201410475021 A CN201410475021 A CN 201410475021A CN 104304103 B CN104304103 B CN 104304103B
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pond
water
lip
fish
rearing
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CN104304103A (en
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刘吉明
袁甜
孙述好
冯春明
李万贺
高沛力
马德利
郑毅
李明
许迪亮
郝永丰
姜景田
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DANDONG CITY AQUATIC TECHNOLOGY PROMOTION STATION
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DANDONG CITY AQUATIC TECHNOLOGY PROMOTION STATION
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

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Abstract

The present invention relates to lipBreed field, be specially a kind of doing sth. in advance and breed, cultivate big specification lipOffspring breeding method, comprises the steps: (1) parent fish rearing: utilize soil pond to carry out the cultivation of parent fish, parent fish specification 1~3kg/ tail, throws something and feeds granular bait for fish by the 2~5% of parent fish body weight, day bait throwing in 2~4 times, micro-running water technology;(2) parent fish accelerating: utilize the rapid feature that heats up early spring, adopts booth daylight to heat, increases illumination, accelerate lipGonad development, in conjunction with stimulation by running water help lipGland is from IV phase to the transition of V phase;(3) artificial induced spawning;(4) artificial insemination and hatching;(5) seed rearing: select soil pond and cement pond clearing both of which to cultivate lipSpray and crow young, soil pond rearing lip

Description

A kind of doing sth. in advance breeds, cultivates big specification lip * offspring breeding method
Technical field
The present invention relates to lip(hu á) breeds field, is specially a kind of doing sth. in advance and breeds, cultivates big specification lipOffspring breeding method.
Background technology
LipHemibarbuslabeo (Pallas), popular name Hemibarbus labeo (Pallas).It is subordinate to Cypriniformes, Cyprinidae, subfamily, Latin generic name: HembarbusBleeker.The whole nation has and is distributed widely, and Liaoning Area is mainly distributed on Yalujing estuary and tributary (river, the big Yanghe River), Hun River, Taizihe River, green stream river etc..LipFine and tender taste, nutritious, rich in aminoacid, vitamin and mineral, it is the high-grade aquatic products deeply liked by consumer.
At present, lipWork raising technology remains in nature at home and fishes Gunther parent fish spawning or fish wild seed, causes lipThe problems such as the seed speed of growth is slow, culture-cycle length.
Summary of the invention
For the problems referred to above, it is an object of the invention to provide a kind of doing sth. in advance and breed, cultivate big specification lipOffspring breeding method, takes plastic greenhouse temperature control, control light, flowing water ripening technique to cultivate parent fish, the hatching of germ cell unsticking, makes the egg reproduction phase shift to an earlier date 15 days, cultivate large size seedling seed then, weed survival rate is greatly improved, be effectively shortened the culture-cycle.
The technical scheme is that
A kind of doing sth. in advance breeds, cultivates big specification lipOffspring breeding method, detailed process is as follows:
(1) parent fish rearing: utilize soil pond to carry out the cultivation of parent fish, parent fish specification 1~3kg/ tail, throw something and feed granular bait for fish by the 2~5% of parent fish body weight, day bait throwing in 2~4 times, micro-running water technology;
(2) parent fish accelerating: utilize the rapid feature that heats up early spring, adopts booth daylight to heat, increases illumination, accelerate lipGonad development, in conjunction with stimulation by running water help lipGonad is from IV phase to the transition of V phase;
(3) artificial induced spawning: observe lipMotion, occur that food ration goes down, chase phenomenon, with visit egg apparatus check part raun ovum grain is full, extruding milter abdominal part have white seminal fluid outflow hasten parturition;
(4) artificial insemination and hatching: take the mode of artificial insemination, do detackifier with yellow mud, concentrates hatching with net cage or couveuse;
(5) seed rearing: select soil pond and cement pond clearing both of which to cultivate lipSpray and crow young, soil pond rearing lipSummer flower and adult fish.
Described breed ahead of time, cultivate big specification lipOffspring breeding method, utilizes booth daylight to heat, and daytime heats up 0.5~1 DEG C naturally, controls water temperature well and keep good water quality in cultivating process, and period, with micro-stimulation by running water parent fish, is effectively improved the incubation rate of parent fish survival rate and germ cell;Water temperature 14 ± 1 DEG C, when feature female, male is obvious, is separated, and point pond is cultivated, it is to avoid female milter lays eggs voluntarily, causes damage, and wants Artificial Control illumination to regulate water temperature in cultivating process, and raun cultivates pond water temperature slightly above male 0.5 DEG C.
Described breed ahead of time, cultivate big specification lipOffspring breeding method, parent fish enters the pond nurturing period, and bait of throwing something and feeding is cultivated 18~22 days, water temperature 16 ± 1 DEG C, observes the ingesting of parent fish, chases phenomenon, and whether ovum grain is full to check female fish egg, starts to hasten parturition and artificial insemination.
Described breed ahead of time, cultivate big specification lipOffspring breeding method, parent fish water for larval nursing first passes around I and II sedimentation tank and precipitates, precipitation suspended material, reach the purpose of primary purifying water quality, seedling rearing room is entered with water after precipitation, water inlet filters microzooplankton and harmful organisms with 100 mesh sieve tulles, processes the harmful bacteria in water with the bromine preparation of the povidone iodine of 0.8~1.2mg/L or 0.8~1.2mg/L simultaneously.
Described breed ahead of time, cultivate big specification lipOffspring breeding method, artificial induced spawning, artificial insemination and artificial incubation are larvae rearing process, specific as follows:
(1) artificial induced spawning
Water temperature 16 ± 1 DEG C, raun ingests and weakens, and it is full to occur mutually chasing phenomenon, abdominal distension and ovum grain, and milter extrudes abdominal part gently, has seminal fluid to flow out, and starts to inject oxytocic drug to parent fish;Take muscle of back or intrathecal: luteotropin releasing hormone d-ala analog LHRH-A2:5~10 μ g/kg and maleic acid DOM DOM:4~8mg/kg, Effect time is 18~30h;
(2) artificial insemination
Extruding raun abdominal part has ovum grain to flow out gently, gets final product artificial insemination;First fish body dry towel is dried, particularly anal fin and afterbody, in order to avoid water droplet enters to cause in basin sperm inactivation, ovum to lose fertility;Being dried by enamel basin, basin inwall coats thin layer vaseline again, and ovum squeezes in enamel basin, clamp-ons seminal fluid, is gently mixed with feather so as to be fertilized;After artificial fertilization, process by conventional method unsticking immediately, and rinse with clear water, to remove the sperm of the upper residual of germ cell, put into after germ cell imbibition in couveuse or net cage and hatch;
(3) artificial incubation
With yellow mud, germ cell unsticking being processed, hatching density is the germ cell after 10000 unstickings placed by each net cage, and cage size is 1 × 0.7 × 1m;Or, germ cell is miniflow water inflation 10~150,000/m of hatching in couveuse3;Water temperature 15~17 DEG C, hatches through 100~120h, and germ cell rupture of membranes successively hatches prelarva, and water temperature 17~20 DEG C, the rupture of membranes time is at 70~90h.
Described breed ahead of time, cultivate big specification lipOffspring breeding method, the process of seed rearing is as follows:
(1) prelarva Tu Chi cultivates
Squab prelarva total length 6~7mm, seedling rearing room water temperature 18~20 DEG C, yolk disappearance initial feeding after 3 days, breeding density is 150~2,250,000 fishes/ha, with wheel animalcule for open-mouthed bait, prelarva culture pond and wheel animalcule culture pond are separated, bean milk culture of rotifera of splashing every day, splash according to the dry Semen Glycines of 2~5kg/ mu according to the fertile degree of pond water;
(2) prelarva cement pit is cultivated
It is 5000 tails/m that spray cultivates density3, spray seed uses wheel animalcule, cladocera, water silk earthworm or artemia to throw something and feed, and different bait add after Vc strengthens 20~30h throws something and feeds, and prelarva cement pit type of rearing cultivates the long 3cm of body in cement pit always;
(3) the young pond rearing of crow
Breeding density 75~1,050,000 fishes/ha, throw something and feed by body weight 8~10% wheel animalcule and bait, through the raising of 20~25 days, seed body, up to 4~5cm, started counting up a point pond and puts into cultivation in culturing pool.
Described breed ahead of time, cultivate big specification lipOffspring breeding method, Vc strengthening adds Vc500~1000 milligram for per kilogram bait.
Described breed ahead of time, cultivate big specification lipOffspring breeding method, carries out large size seedling seed cultivation after seed rearing, detailed process is as follows:
(1) pond treatment
Pond area 666~1200m2, the depth of water 1~1.2m;Put fingerling basal dressing, every 666m before 5~7 days in a suitable place to breed2Execute the fertilizer 150~200kg become thoroughly decomposed;
(2) feeding and management
Putting seed size in a suitable place to breed is 4~5cm, and density is 6000~8000 tails/666m2, water silk earthworm of throwing something and feeding when just having entered pond mixes granular bait for fish, and granular bait for fish of throwing something and feeding completely after adapting to, is thrown something and fed by the 7~8% of fish body weight at every day 2~3 times, and autumn in end of the year sheet seed body weight reaches 80~90g.
Described breed ahead of time, cultivate big specification lipOffspring breeding method, the part by weight of water silk earthworm and bait is 1:4~6.
Described breed ahead of time, cultivate big specification lipOffspring breeding method, the flow of miniflow water is 40~60L/min.
The present invention is applied to lipArtificial breeding technology has the following advantages and beneficial effect:
1, the present invention can make lipThe egg reproduction phase shift to an earlier date 15 days, cultivate the body weight large size seedling seed more than 60 grams then, it is possible to increase substantially weed survival rate, effective Shortening culturing period.
2, early stage in April artificial breeding, is improved by sun light green house and cultivates water temperature, illumination, reinforcement nutrition stimulation by running water, promote parent fish gonad development, and the artificial breeding time can shift to an earlier date more than 15 days.Adopt the stimulation by running water of stimulation by running water, particularly larger flow to lip aborningGonad development there is obvious facilitation, now become induction the sexually matured steps necessary of parent fish.
3, method for culturing seedlings, lip ahead of time are adoptedSeed body length can on average increase 1cm, and body weight increases 5g, and the cultivation phase extends more than 20 days.Raising technology ahead of time, it is possible to provide large size seedling seed for producing, extends the cultivation phase, forms marketable fish ahead of time.
Detailed description of the invention
In a specific embodiment, the present invention breeds ahead of time, cultivates big specification lipThe process of offspring breeding method is as follows:
1. parent fish rearing: utilize soil pond to carry out the cultivation of parent fish, parent fish specification 1~3kg/ tail, throw something and feed granular bait for fish by the 2~5% of parent fish body weight, day bait throwing in 2~4 times, micro-running water technology.
2. parent fish accelerating: utilize the rapid feature that heats up early spring, adopts booth daylight to heat, increases illumination (the Fish laid eggs in spring and summer sky, the sunshine-duration extends than naturalness, just can do sth. in advance sexual maturity and lay eggs), accelerate lipGonad development, in conjunction with stimulation by running water help lipGonad is from IV phase to the transition of V phase.The north utilizes booth daylight to heat, it is possible to subsist than natural water area accumulation accumulated temperature many 30, and Early-breeding breeds 15 days.
3. artificial induced spawning: observe lipMotion, occur that food ration goes down, chase phenomenon on a small quantity, checking part raun with visiting egg apparatus ovum grain is full, extruding milter abdominal part has a small amount of white seminal fluid to flow out and can hasten parturition.
4. artificial insemination and hatching: take the mode of artificial insemination, does detackifier with yellow mud, and germ cell can use net cage, fiberglass couveuse or cement pit to concentrate hatching.
5. seed rearing: select soil pond and cement pond clearing both of which to cultivate lipSpray and crow young.Soil pond rearing lipSummer flower and adult fish, attempt different breeding pattern, control and prevention of disease and suitable bait.
In the present invention, relational language is explained as follows:
1, accumulated temperature:
Parent fish is in growth promoter period, do not require nothing more than certain temperature (height of temperature), but also need certain heat summation, this heat summation usually with this period water temperature day by day accumulated value represent, this accumulated value is just called accumulated temperature, and unit is to subsist.
2, spray, crow son, summer flower and autumn sheet:
Fresh-water fish-culture term.Fry is also spray, fish fry etc., generally refers to the prelarva that just hatching is later.When fry grows to 1.5~1.8 cm, young also referred to as crow.When fry grows to 4~5 cm, because going out the pool on the occasion of summer, therefore claim summer flower.To autumn, through cultivating, when summer flower is grown up to 8~10 cm, just it is " autumn flower ", also has and be called " autumn sheet ".
3, miniflow water: flow 40~60L/min.
4, Effect time: (counting) to the required time that starts to oestrus after parent fish has injected odinagogue when completing with last injection is Effect time (18~30h).
The present invention is described in more detail by the examples below.
Embodiment 1
In the present embodiment, breed ahead of time, cultivate big specification lipOffspring breeding method, specifically comprises the following steps that
1. parent fish rearing and accelerating
Fish for the lip of nature and artificial cultivation, it is preferable that body weight 1~3kg body as parent fish, carries out reinforced cultivating without disability after screening.Utilize 8m × 8m=64m2Cement pit carries out the cultivation of parent fish, depth of water 1.4m, and parent fish 10~15 tail is put in every pond in a suitable place to breed.Clear up every day at the bottom of pond once, throw something and feed by the 3~5% of parent fish the body weight minced fish of iced trash fish processing or artifical compound feed, day bait throwing in three times.
April 5 started with booth daylight and heats, daytime can heat up 0.5~1 DEG C naturally, controlling water temperature in cultivating process well and keep good water quality, period stimulates parent fish with miniflow water (flow 50L/min), it is possible to be effectively improved the incubation rate of parent fish survival rate and germ cell.Parent fish to prevent water temperature change too fast after entering seedling rearing room or booth, causes parent fish gonad to be degenerated or dead.Water temperature 14 ± 1 DEG C, when feature female, male is obvious, is separated, and point pond is cultivated, it is to avoid female milter lays eggs voluntarily, causes damage, and wants Artificial Control illumination to regulate water temperature in cultivating process, and raun cultivates pond water temperature slightly above male 0.5 DEG C.
Parent fish enters the pond nurturing period, conventional high-quality bait of throwing something and feeding, and cultivates 20 days, water temperature 16 ± 1 DEG C, the ingesting of observation parent fish, chases phenomenon, and whether ovum grain is full to check female fish egg, starts to hasten parturition and artificial insemination.
2. water for larval nursing processes
Water for larval nursing first passes around I and II sedimentation tank and precipitates, precipitation suspended material, reach the purpose of primary purifying water quality, seedling rearing room is entered with water after precipitation, water inlet filters microzooplankton and harmful organisms with 100 mesh sieve tulles, processes the harmful bacteria in water with the bromine preparation of the povidone iodine (PV iodine) of 1mg/L or 1mg/L (such as DBDMH or bromine chlorine hydantoin etc.) simultaneously.
3. larvae rearing
(1) artificial induced spawning
Artificial induced spawning: spawning rate is high, and ovum quality is excellent, it is possible to meet large-scale production needs.Thus, test thereafter adopt which obtain germ cell.
Water temperature 16 ± 1 DEG C, raun ingests and weakens, and it is full to occur mutually chasing phenomenon, abdominal distension and ovum grain, and milter extrudes abdominal part gently, has seminal fluid to flow out, and can start to inject oxytocic drug to parent fish.Taking muscle of back or intrathecal method, the oxytocic drug of injection is: luteotropin releasing hormone d-ala analog (LHRH-A2) 5~10 μ g/kg and maleic acid DOM (DOM) 4~8mg/kg, Effect time is 18~30h.
In order to prevent ovum overdone, moving on to net cage waiting to produce parent fish, and check in time after hastening parturition, selecting the best opportunity to carry out artificial insemination, the parent fish of body weight 1~3kg, eggs on average amount is 2~60,000.
(2) artificial insemination
Extruding raun abdominal part has ovum grain to flow out gently, gets final product artificial insemination.First fish body dry towel is dried, particularly anal fin and afterbody, in order to avoid water droplet enters to cause in basin sperm inactivation, ovum to lose fertility.Being dried by enamel basin, basin inwall coats thin layer vaseline again, and ovum squeezes in enamel basin, clamp-ons several seminal fluid, is gently mixed with feather so as to be fertilized.For convenient operation, in advance seminal fluid syringe sucking-off being placed in refrigerator cold preservation standby, the seminal fluid deposited can preserve 5~7h.After artificial fertilization, processing by conventional method unsticking immediately, and repeatedly rinse with clear water, to remove the sperm of the upper residual of germ cell, after germ cell imbibition, (ovum footpath is about 2.6mm) is put in couveuse or net cage and is hatched.
(3) artificial incubation
With yellow mud, germ cell unsticking is processed, couveuse is inflated hatching method.Cage size is 10000, germ cell after 1 × 0.7 × 1m can hatch a unsticking;In couveuse, miniflow water (flow 50L/min) inflates hatching density is 10~150,000/m3.Water temperature 15~17 DEG C, hatches through 110h, and germ cell rupture of membranes successively hatches prelarva, and water temperature 17~20 DEG C, the rupture of membranes time is at 80h.To carry out during hatching and disinfect, it is to avoid growing of water mo(u)ld.
4. seed rearing
(1) prelarva Tu Chi cultivates
Squab prelarva total length 6.5mm, seedling rearing room water temperature 18~20 DEG C, yolk disappearance initial feeding (natural temperature needs 5 days) after 3 days, breeding density is 150~2,250,000 fishes/ha (ha).Doing a good job of it two links in cultivation, one thoroughly to clean up the pond elimination harmful organisms, keeps water quality pure and fresh;The two open-mouthed bait supplies ensureing high-quality, wheel animalcule is best open-mouthed bait.High-quality, agreeable to the taste bait can improve unit of water body output of seedling, there is special cultivation pond, prelarva culture pond and wheel animalcule culture pond are separated, bean milk culture of rotifera of splashing every day, fertile degree according to pond water is splashed according to 2~5kg/ mu (dry Semen Glycines), it is possible to be effectively improved wheel animalcule quantity.
(2) prelarva cement pit is cultivated
It is 5000 tails/m that spray cultivates density3, spray seed uses the different bait such as wheel animalcule, cladocera, water silk earthworm, artemia to throw something and feed after adding Vc strengthening (per kilogram bait adds 500~1000 milligrams of Vc) 24h, increases attractant and the nutrition of bait, is effectively improved weed survival rate.Prelarva cement pit type of rearing, it is possible to cultivate long more than the 3cm of body in cement pit always and sell.
(3) the young pond rearing of crow
Clean up the pond rich water, storage pond, holding pond area 1~3 mu, first pond is carried out smooth, throw in bio-fertilizer water preparation, and rich water is carried out strict filtration, sterilization, it is prevented that pest and disease damage enters pond, improves the young survival rate of crow.Breeding density 75~1,050,000 fishes/ha (ha), throws something and feeds wheel animalcule be domesticated for normal miniature high-quality bait gradually by body weight 8~10%, and through the raising of 20~25 days, seed body, up to 4~5cm, started counting up a point pond and puts in culturing pool and cultivate.
5. prevention of damage by disease
Along with the raising of water temperature, various antibacterials, Trichodina, fish molds amount reproduction, immerse in ovum and fish body, make ovum permeability be deteriorated lethal.Applying 1mg/L New-type wide-spectrum germ killing drugs (DBDMH) in every 5 sky and water, pre-disease prevention occurs.
6. large size seedling seed is cultivated
(1) pond treatment
Thorough disinfection pond, with 666~1200m2Area is advisable, the depth of water 1~1.2m.Put fingerling basal dressing, every 666m before 5~7 days in a suitable place to breed2Execute the fertilizer 150~200kg become thoroughly decomposed.
(2) feeding and management
Putting seed size in a suitable place to breed is 4~5cm, and density is 6000~8000 tails/666m2, the water silk earthworm having thrown something and fed a small amount of when just having entered pond mixes granular bait for fish (part by weight of water silk earthworm and bait is 1:5), granular bait for fish of throwing something and feeding completely after adapting to, and every day 2~3 times, throws something and feeds by the 7~8% of fish body weight, and autumn in end of the year sheet seed body weight reaches 85g.
(3) overwinter management
LipThe adaptability of low temperature is stronger than Cyprinus carpio, observe through in a few years, during low water temperature in winter, lipSeedling also has motion, and the general water surface will not freeze, and does not therefore need heavy net cage, it is possible to reduce production link, reduces labor intensity.
It is noted that regulating water quality before Wintering, cultivate phytoplankton, keep good water quality and sufficient oxygen, create good environment of surviving the winter.

Claims (9)

1. do sth. in advance to breed, cultivate big specification lip for one kindOffspring breeding method, it is characterised in that detailed process is as follows:
(1) parent fish rearing: utilize soil pond to carry out the cultivation of parent fish, parent fish specification 1~3kg/ tail, throw something and feed granular bait for fish by the 2~5% of parent fish body weight, day bait throwing in 2~4 times, micro-running water technology;
(2) parent fish accelerating: utilize the rapid feature that heats up early spring, adopts booth daylight to heat, increases illumination, accelerate lipGonad development, in conjunction with stimulation by running water help lipGonad is from IV phase to the transition of V phase;
(3) artificial induced spawning: observe lipMotion, occur that food ration goes down, chase phenomenon, with visit egg apparatus check part raun ovum grain is full, extruding milter abdominal part have white seminal fluid outflow hasten parturition;
(4) artificial insemination and hatching: take the mode of artificial insemination, do detackifier with yellow mud, concentrates hatching with net cage or couveuse;
(5) seed rearing: adopt prelarva soil pond rearing, prelarva cement pond clearing to cultivate and crow son's pond rearing, soil pond rearing lipSummer flower and adult fish;
Utilizing booth daylight to heat, daytime heats up 0.5~1 DEG C naturally, controls water temperature well and keep good water quality in cultivating process, and period, with micro-stimulation by running water parent fish, is effectively improved the incubation rate of parent fish survival rate and germ cell;Water temperature 14 ± 1 DEG C, when feature female, male is obvious, is separated, and point pond is cultivated, it is to avoid female milter lays eggs voluntarily, causes damage, and wants Artificial Control illumination to regulate water temperature in cultivating process, and raun cultivates pond water temperature slightly above male 0.5 DEG C.
2. doing sth. in advance described in claim 1 breeds, cultivates big specification lipOffspring breeding method, it is characterised in that parent fish enters the pond nurturing period, bait of throwing something and feeding is cultivated 18~22 days, water temperature 16 ± 1 DEG C, observes the ingesting of parent fish, chases phenomenon, and whether ovum grain is full to check female fish egg, starts to hasten parturition and artificial insemination.
3. doing sth. in advance described in claim 1 breeds, cultivates big specification lipOffspring breeding method, it is characterized in that, parent fish water for larval nursing first passes around I and II sedimentation tank and precipitates, precipitation suspended material, reach the purpose of primary purifying water quality, entering seedling rearing room with water after precipitation, water inlet filters microzooplankton and harmful organisms with 100 mesh sieve tulles, processes the harmful bacteria in water with the bromine preparation of the povidone iodine of 0.8~1.2mg/L or 0.8~1.2mg/L simultaneously.
4. doing sth. in advance described in claim 1 breeds, cultivates big specification lipOffspring breeding method, it is characterised in that artificial induced spawning, artificial insemination and artificial incubation are larvae rearing process, specific as follows:
(1) artificial induced spawning
Water temperature 16 ± 1 DEG C, raun ingests and weakens, and it is full to occur mutually chasing phenomenon, abdominal distension and ovum grain, and milter extrudes abdominal part gently, has seminal fluid to flow out, and starts to inject oxytocic drug to parent fish;Take muscle of back or intrathecal: luteotropin releasing hormone d-ala analog LHRH-A2:5~10 μ g/kg and maleic acid DOM DOM:4~8mg/kg, Effect time is 18~30h;
(2) artificial insemination
Extruding raun abdominal part has ovum grain to flow out gently, gets final product artificial insemination;First fish body dry towel is dried, particularly anal fin and afterbody, in order to avoid water droplet enters to cause in basin sperm inactivation, ovum to lose fertility;Being dried by enamel basin, basin inwall coats thin layer vaseline again, and ovum squeezes in enamel basin, clamp-ons seminal fluid, is gently mixed with feather so as to be fertilized;After artificial fertilization, with yellow mud, germ cell unsticking is processed immediately, and rinse with clear water, to remove the sperm of the upper residual of germ cell, put into after germ cell imbibition in couveuse or net cage and hatch;
(3) artificial incubation
Hatching density is the germ cell after 10000 unstickings placed by each net cage, and cage size is 1 × 0.7 × 1m;Or, germ cell is miniflow water inflation 10~150,000/m of hatching in couveuse3;Water temperature 15~17 DEG C, hatches through 100~120h, and germ cell rupture of membranes successively hatches prelarva, and water temperature 17~20 DEG C, the rupture of membranes time is at 70~90h.
5. doing sth. in advance described in claim 1 breeds, cultivates big specification lipOffspring breeding method, it is characterised in that the process of seed rearing is as follows:
(1) prelarva soil pond rearing
Squab prelarva total length 6~7mm, seedling rearing room water temperature 18~20 DEG C, yolk disappearance initial feeding after 3 days, breeding density is 150~2,250,000 fishes/ha, with wheel animalcule for open-mouthed bait, prelarva culture pond and wheel animalcule culture pond are separated, bean milk culture of rotifera of splashing every day, splash according to the dry Semen Glycines of 2~5kg/ mu according to the fertile degree of wheel animalcule culture pond water;
(2) prelarva cement pond clearing is cultivated
It is 5000 tails/m that spray cultivates density3, spray seed uses wheel animalcule, cladocera, water silk earthworm or artemia to throw something and feed, and different bait add after Vc strengthens 20~30h throws something and feeds, and prelarva cement pond clearing type of rearing cultivates the long 3cm of body in cement pond clearing always;
(3) the young pond rearing of crow
Breeding density 75~1,050,000 fishes/ha, throw something and feed by body weight 8~10% wheel animalcule and bait, through the raising of 20~25 days, seed body, up to 4~5cm, started counting up a point pond and puts into cultivation in soil pond.
6. doing sth. in advance described in claim 5 breeds, cultivates big specification lipOffspring breeding method, it is characterised in that Vc strengthening adds Vc500~1000 milligram for per kilogram bait.
7. doing sth. in advance described in claim 1 breeds, cultivates big specification lipOffspring breeding method, it is characterised in that carrying out large size seedling seed cultivation after the young pond rearing of crow, detailed process is as follows:
(1) pond treatment
Pond area 666~1200m2, the depth of water 1~1.2m;Put fingerling basal dressing, every 666m before 5~7 days in a suitable place to breed2Execute the fertilizer 150~200kg become thoroughly decomposed;
(2) feeding and management
Putting seed size in a suitable place to breed is 4~5cm, and density is 6000~8000 tails/666m2, water silk earthworm of throwing something and feeding when just having entered pond mixes granular bait for fish, and granular bait for fish of throwing something and feeding completely after adapting to, is thrown something and fed by the 7~8% of fish body weight at every day 2~3 times, and autumn in end of the year sheet seed body weight reaches 80~90g.
8. doing sth. in advance described in claim 7 breeds, cultivates big specification lipOffspring breeding method, it is characterised in that the part by weight of water silk earthworm and bait is 1:4~6.
9. doing sth. in advance described in claim 1 or 5 breeds, cultivates big specification lipOffspring breeding method, it is characterised in that the flow of miniflow water is 40~60L/min.
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