CN101622974A - Soilless Nereid larva breeding method - Google Patents

Soilless Nereid larva breeding method Download PDF

Info

Publication number
CN101622974A
CN101622974A CN200910040254A CN200910040254A CN101622974A CN 101622974 A CN101622974 A CN 101622974A CN 200910040254 A CN200910040254 A CN 200910040254A CN 200910040254 A CN200910040254 A CN 200910040254A CN 101622974 A CN101622974 A CN 101622974A
Authority
CN
China
Prior art keywords
larva
nereid
soilless
algae
breeding
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN200910040254A
Other languages
Chinese (zh)
Other versions
CN101622974B (en
Inventor
李长玲
罗杰
黄翔鹄
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Guangdong Ocean University
Original Assignee
Guangdong Ocean University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Guangdong Ocean University filed Critical Guangdong Ocean University
Priority to CN2009100402545A priority Critical patent/CN101622974B/en
Publication of CN101622974A publication Critical patent/CN101622974A/en
Application granted granted Critical
Publication of CN101622974B publication Critical patent/CN101622974B/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Abstract

The invention relates to a soilless Nereid larva breeding method. According to the method, with 27-29 DEG C of water temperature, 7.8-8.6 of pH and 28-30 of salinity, larva breeding water undergoes dark precipitation and secondary sand filtration and is then filtered for further use by a 300-mesh bolting-silk bag; large and vigorous heteronereis bodies with no exterior wounds are selected and positioned in a plastic bucket containing 0.5 m3 of sea water for oviposition and insemination according to a proportion of 3:1 between the female and the male heteronereis bodies; oosperms undergo three times of egg washing and then are moved into a larva breeding pond; larva in a roaming period are mainly fed with chlamydomonas together with a certain amount of artificially mixed feed; when the larva grow into five-setiger nectochaeta larva, gracilaria is put in the pond as an attachment substrate, and a small amount of fish paste is also fed; and during the Nereid larva breeding, according to larva growing periods, the light illumination is controlled in a range of 300-1000 Lux. When the gracilaria is used for replacing silt as the Nereid perching place, as the gracilaria can utilize the metabolic products of the Nereids as the nutrients to promote self growth and is easily cleaned, the living environment of the larva can be bettered, the diseases and the damage to the larva during larva generation can be reduced, and the survival rate of the larva can be improved.

Description

Soilless Nereid larva breeding method
Technical field
The invention belongs to the agriculture, forestry, animal husbandry, fisheries technical field, particularly a kind of soilless Nereid larva breeding method.
Background technology
Clam worm (Perinereis aibuhitensis Gnlbe) belongs to Annelida, polychaeta (Polychaeta), clam worm order (Nereidida), Nereidae (Neteididae), clam worm subfamily (Neteidinae), rich in proteins, eicosapentaenoic acid, economic worth is high.The fertile body of its meat is big, belongs to superior bait, and the title of " omnipotent bait " is arranged, and also is the good kind of foreign exchange earning, about 10,000 dollars per ton; Secondly, it is the high-quality fresh living bait of fish and shrimp crab class etc., or as the additive and the bait of aquatic product matched bait, can save daily ration, feeding quantity in the shrimp pond, and clarification water quality reduces prawn disease; It perches the river mouth simultaneously, and its larva and adult all can be used as the good biological index that detects water pollution.China is coastal abundant beach resource, and the nereis culture potentiality are very big.
At present the artificial breeding of clam worm and culturing mainly concentrates on Fujian, Jiangsu, Zhejiang one and is with coastally, and southern Guangdong, Guangxi etc. are not seen relevant research report.In all domestic and international research reports, traditional clam worm artificial breeding technique all is to treat larvae development to 5 joint young later stage lay fine sand at the bottom of the nursery pond pond just, as the adherance of larva.But fine sand is made the adherance of larva, there is following shortcoming: the one, excreta and residual bait owing to seedling self in cultivating process are embedded in the sand, clean difficulty and cause disease easily, the 2nd, difficulty is very big when emerging, at first emerge net cage because the grains of sand are spilt, can only adopt 80 mesh sieve thin,tough silk, and the clam worm larva is gentle, the soft vermiform that is, less larva will escape from the net cage of emerging.In order to make the grains of sand spill net cage, will constantly rock net cage on the other hand, this moment, the clam worm seedling was clipped in the grains of sand very easily injuredly, had reduced the breed survival rate.Therefore, thereby the improvement of clam worm seedling adherance being replaced traditional grains of sand, is the problem of answering primary study from now in the clam worm artificial breeding.
Summary of the invention
The objective of the invention is in order to overcome the deficiency that above-mentioned prior art exists, according to the certain object of clam worm young needs when the metamorphosis as this life habit of its adherance and characteristics, and consider traditional grains of sand adherance to clean difficulty and cause disease easily and the larva shortcoming of damage easily when emerging, a kind of soilless Nereid larva breeding method is provided.
In order to realize the foregoing invention purpose, the technical scheme that the present invention takes is that this soilless Nereid larva breeding method may further comprise the steps:
1, culture facility and water: nursery pond is the about 30m of floor space 2, the dark 1.5m in pond, bottom have the cement pit of osculum; Algae culture pond floor space 10m 2, dark 1.2m cultures pond and algae culture pond and lays gas stone; Grow seedlings 27 ℃~30 ℃ of the water temperatures of seawater, pH7.8~8.6, salinity 28~30, through obscure shallow lake, the husky filter of secondary, it is as follows specifically to handle the path: husky → 300 mesh sieve thin,tough silk bag → nursery ponds or the algae culture pond of filtering of the husky filter → secondary of seawater → obscure shallow lake → one-level.
2, selection of close silkworm and fertilized egg hatching: selection is individual greatly, appearance does not have the good heteronereis of the vigor of injuring (the ripe clam worm that will soon breed), places in ratios female, male 3: 1 to fill 0.5m 3The Plastic Drum of seawater, little qi of chong channel ascending adversely, treat that heteronereis is laid eggs fully and arranged essence after, after leaving standstill a period of time fertilized egg and sinking,, add filtering sea again with the siphon method top seawater of draining, repeating to operate three times like this can the unnecessary seminal fluid of flush away.Fertilized egg through above-mentioned processing is moved into nursery pond, and density is controlled at 5~8/mL.Because clam worm fertilized egg is a demersal egg,, regulate aeration quantity in order to prevent the bottom anoxic.
3, the selection of adherance and input: when just joint nectochaeta larva phase of paedomorphosis to five, the cilium on its body reduces no longer swimming gradually, begins to enter the end life of crawling of dwelling, and needs in time to throw in adherance.Selection is grown in than the adherance of the fragrant plant mentioned in ancient texts in the high salinity seawater as the young.Other biologicals such as being attached to little shellfish on the fragrant plant mentioned in ancient texts, infusorians is cleaned up, and with after the formaldehyde thorough disinfection it being rendered to growth to five just at the bottom of the nursery pond pond in joint nectochaeta larva later stage, supply volume is 2.5~3 jin/m 2(the fragrant plant mentioned in ancient texts weight that nothing is dripped).At this moment to control aeration quantity, because the too big fragrant plant mentioned in ancient texts of aeration quantity can be floated.
The larva management:
4, daily management: squab three have just saved nectochaeta larva with the nutriment of the yolk sac in the body as self, grow to four firm joint nectochaeta larva phases, and digestive tract begins to form, yolk sac disappearance, the bait of need in time throwing something and feeding.The 8:00 of every day, 15:00 and 21:00 throw something and feed, and the unit cell algae of mainly throwing something and feeding (Zhanjiang Isochrysis galbana, flat algae and cloud tail algae) hold concurrently to be thrown some artifical compound feeds, and feeding volume is the ingesting situation of the young and deciding according to the observation.Treat that young battalion dwells after the life a spot of fish meat emulsion of throwing something and feeding the end; Described artifical compound feed is prawn slice and BB powder.
In three just joint nectochaeta larvas to five just joint nectochaeta larva early stages, the life of swimming of young battalion is sought the end to dwell and crawl to live afterwards.In the stage of swimming, change water about 1/3~1/2 with mesh bag every day; All seek the end when the young and dwell and crawl after the life, direct row's upper water when changing water, quantity of exchanged water is more than 50%.Because the illumination that the growth needs of fragrant plant mentioned in ancient texts is certain, according to the developmental stage timely adjustment intensity of illumination of the young, the stage of swimming is controlled at 300~600Lux and is advisable in seedling raising process, and life back illumination is dwelt the end between 400~1000Lux in battalion.
It is dark to add seawater 80cm in the described algae culture pond, and with strong chlorine oil sterilization back fertilising, the nutritive salt prescription of cultivating the unit cell algae is NaNO 350ppm, KH 2PO 45ppm, FeCl 30.5ppm, connect the algae kind afterwards; Described unit cell algae is Zhanjiang Isochrysis galbana, flat algae and cloud tail algae.
Soilless Nereid larva breeding method of the present invention is that traditional adherance is the grains of sand to the improvement of young adherance in the clam worm tradition artificial breeding, and that the grains of sand clean difficult breeding environment is contaminated easily, diseases induced generation, and the damage young easily when emerging.The present invention's tangleweed---fragrant plant mentioned in ancient texts substitutes silt, can optimize the breeding environment of clam worm.Because fragrant plant mentioned in ancient texts is as the habitat of clam worm, not only clean easily, improve environment, and the branch of fragrant plant mentioned in ancient texts is various, have enough spaces to perch for clam worm, fragrant plant mentioned in ancient texts can utilize the nutriment environment purification of the excreta of clam worm as self simultaneously, promotes the growth of fragrant plant mentioned in ancient texts and increases output, fragrant plant mentioned in ancient texts can be used as commodity selling, increases economic benefit.The present invention technology, when improving the clam worm survival rate of seedling, seed and fragrant plant mentioned in ancient texts need not peel off, and directly the seed that is attached on the fragrant plant mentioned in ancient texts are cultured, and reduce the injury to larva.
Concrete embodiment
Soilless Nereid larva breeding method of the present invention may further comprise the steps:
1, the about 10m of algae culture pond floor space 2, dark 1.2m, lay 6 of gas exhausting stones, add water 80cm and apply fertilizer after with strong chlorine oil sterilization seawater, the nutritive salt prescription of cultivating the unit cell algae is NaNO 350ppm, KH 2PO 45ppm, FeCl 30.5ppm, connect algae kind (kind has flat algae, Zhanjiang Isochrysis galbana and cloud tail algae) afterwards.
2, select individual big, appearance not have the good heteronereis of the vigor of injuring (the ripe clam worm that is about to breeding), place and fill 0.5m by female, 3: 1 ratios of hero 3In the Plastic Drum of seawater, regulate the qi of chong channel ascending adversely amount and be little shape that boils, treat that heteronereis is laid eggs fully and arranged essence after, after leaving standstill a period of time fertilized egg sinking, with the drain seawater on upper strata of siphon method, add filtering sea again, repeating operation like this three times can the unnecessary seminal fluid of flush away.Fertilized egg through above-mentioned processing moves into the about 30m of floor space 2, dark 1.5m, bottom have the nursery pond of osculum, pond portion is by 1 gas stone/5~6m 2Quantity lay, 27 ℃~30 ℃ of the water temperatures of the seawater of growing seedlings, pH7.8~8.6, salinity 28~30, through obscure shallow lake, the husky filter of secondary, it is as follows specifically to handle the path: husky → 300 mesh sieve thin,tough silk bag → nursery ponds of filtering of the husky filter → secondary of seawater → obscure shallow lake → one-level; Incubating oosperm density is controlled at 5~8/mL.Because clam worm fertilized egg is a demersal egg, in order to prevent the bottom anoxic, its aeration quantity ratio is big slightly when laying eggs.
3, when 27~29 ℃ of water temperatures, fertilized egg need be hatched three firm joint nectochaeta larvas approximately in 42 hours, and squab three have just saved nectochaeta larvas with the nutriment of the yolk sac in the body as self, and growth to four firm joint nectochaeta larva digestive tracts begin to form, yolk sac disappears, the bait of need in time throwing something and feeding.The 8:00 of every day, 15:00 and 21:00 throw something and feed, and the unit cell algae of mainly throwing something and feeding (Zhanjiang Isochrysis galbana, flat algae and cloud tail algae) hold concurrently to be thrown some artifical compound feeds (prawn slice and BB powder), and feeding volume is the ingesting situation of the young and deciding according to the observation.
4, when just joint nectochaeta larva phase of paedomorphosis to five, the cilium on its body reduces no longer swimming gradually, begins to enter the end life of crawling of dwelling, and needs in time to throw in adherance.Other biologicals such as being attached to little shellfish on the fragrant plant mentioned in ancient texts, infusorians is cleaned up, with after the formaldehyde thorough disinfection its even lay at the bottom of the pond, supply volume is 2.5~3 jin/m 2(the fragrant plant mentioned in ancient texts weight that nothing is dripped).At this moment to note controlling aeration quantity, because the too big fragrant plant mentioned in ancient texts of aeration quantity can be floated.Treat the young all seek the end dwell crawl the life after, except that throw something and feed unit cell algae, artifical compound feed, a spot of fish meat emulsion of throwing something and feeding simultaneously.
5, just saved nectochaeta larva in earlier stage at three firm joint nectochaeta larvas to five, the young is sought the life of swimming, and change water about 1/3~1/2 with mesh bag every day; All seek the end when the young and dwell and crawl after the life, direct row's upper water when changing water, quantity of exchanged water is more than 50%.Because the illumination that the growth needs of fragrant plant mentioned in ancient texts is certain, according to the developmental stage timely adjustment intensity of illumination of the young, the stage of swimming is controlled at 300~600Lux and is advisable in seedling raising process, and battalion impinges upon between 400~1000Lux in life time of dwelling at the end.Cultivate the time in the young later stage, the density of the running check young suitably increases the quantity of fragrant plant mentioned in ancient texts.

Claims (7)

1, a kind of soilless Nereid larva breeding method is characterized in that according to the following steps:
(1) culture facility and water: nursery pond is the about 30m of floor space 2, the dark 1.5m in pond, bottom have the cement pit of osculum; Algae culture pond floor space 10m 2, dark 1.2m cultures pond and algae culture pond and lays gas stone; Grow seedlings 27 ℃~30 ℃ of the water temperatures of seawater, pH7.8~8.6, salinity 28~30 after obscure shallow lake, the husky filter of secondary, is used behind 300 mesh sieve thin,tough silk bag filtrations again;
(2) selection of close silkworm and fertilized egg hatching: selection is individual greatly, appearance does not have the good heteronereis of the vigor of injuring, and places in ratios female, male 3: 1 to fill 0.5m 3The Plastic Drum of seawater, little qi of chong channel ascending adversely treats that heteronereis lays eggs fully and arrange smartly, takes out close silkworm, moves into nursery pond after washing ovum three times, incubating oosperm density is controlled at 5~8/mL, regulates aeration quantity;
(3) squab three just saved nectochaeta larva with the nutriment of the yolk sac in the body as self, growth to four is the joint nectochaeta larva phase just, digestive tract begins to form, yolk sac disappears, the bait of need in time throwing something and feeding, the 8:00 of every day, 15:00 and 21:00 throw something and feed, the unit cell algae of mainly throwing something and feeding, hold concurrently and throw some artifical compound feeds, feeding volume is the ingesting situation of the young and deciding according to the observation, and when just joint nectochaeta larva phase of paedomorphosis to five, the cilium on its body reduces no longer swimming gradually, begin to enter the end life of crawling of dwelling, need in time to throw in adherance;
(4) in three just joint nectochaeta larvas to five just joint nectochaeta larva early stages, the life of swimming of young battalion is sought the end to dwell and crawl to live afterwards, and in the stage of swimming, change water about 1/3~1/2 with mesh bag every day; All seek the end when the young and dwell and crawl after the life, direct row's upper water when changing water, quantity of exchanged water is more than 50%.
2, according to the described soilless Nereid larva breeding method of claim 1, it is characterized in that: described adherance is a fragrant plant mentioned in ancient texts, and the fragrant plant mentioned in ancient texts of selecting should be grown in the higher environment of salinity, washes the harmful organisms that is attached on the fragrant plant mentioned in ancient texts simultaneously, and with throwing in after the formaldehyde thorough disinfection, supply volume is 2.5~3 jin/m 2, refer to not have the fragrant plant mentioned in ancient texts weight of dripping.
3, according to the described soilless Nereid larva breeding method of claim 1, it is characterized in that: the young is all sought the end and dwells and crawl except that throw something and feed unit cell algae and artifical compound feed, hold concurrently to throw a spot of fish meat emulsion simultaneously after the life.
4, according to the described soilless Nereid larva breeding method of claim 2, it is characterized in that: the illumination of fragrant plant mentioned in ancient texts growth, according to the developmental stage timely adjustment intensity of illumination of the young, the stage of swimming is controlled at 300~600Lux and is advisable in seedling raising process, and battalion impinges upon between 400~1000Lux in life time of dwelling at the end.
5, according to the described soilless Nereid larva breeding method of claim 1, it is characterized in that: it is dark to add seawater 80cm in the described algae culture pond, and with strong chlorine oil sterilization back fertilising, the nutritive salt prescription of cultivating the unit cell algae is NaNO 350ppm, KH 2PO 45ppm, FeCl 30.5ppm, connect the algae kind afterwards.
6, according to claim 1 or 5 described soilless Nereid larva breeding methods, it is characterized in that: described unit cell algae is Zhanjiang Isochrysis galbana, flat algae and cloud tail algae.
7, according to the described soilless Nereid larva breeding method of claim 1, it is characterized in that: described artifical compound feed is prawn slice and BB powder.
CN2009100402545A 2009-06-09 2009-06-09 Soilless Nereid larva breeding method Expired - Fee Related CN101622974B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN2009100402545A CN101622974B (en) 2009-06-09 2009-06-09 Soilless Nereid larva breeding method

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN2009100402545A CN101622974B (en) 2009-06-09 2009-06-09 Soilless Nereid larva breeding method

Publications (2)

Publication Number Publication Date
CN101622974A true CN101622974A (en) 2010-01-13
CN101622974B CN101622974B (en) 2011-05-18

Family

ID=41519088

Family Applications (1)

Application Number Title Priority Date Filing Date
CN2009100402545A Expired - Fee Related CN101622974B (en) 2009-06-09 2009-06-09 Soilless Nereid larva breeding method

Country Status (1)

Country Link
CN (1) CN101622974B (en)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103563861A (en) * 2013-11-07 2014-02-12 东营振宇水产资源开发有限公司 Perinereis aibuhitensis totally-artificial breeding technology
CN103583439A (en) * 2013-11-20 2014-02-19 文登市水产技术推广站 Method for improving egg washing efficiency of bay scallops
CN103636569A (en) * 2013-11-27 2014-03-19 安徽省郎溪县水产管理站 Method for cultivating initial feed of Pseudorasbora parva
CN103651273A (en) * 2013-12-30 2014-03-26 盐城工学院 Perinereis aibuhitensis accumulated-water-free saturated wet soil cultivation method
CN106577589A (en) * 2016-12-09 2017-04-26 国家海洋局北海环境监测中心 A clam worm trapping apparatus and a method of trapping clam worms through the apparatus
CN109090050A (en) * 2018-08-29 2018-12-28 中国水产科学研究院黄海水产研究所 A kind of nereis culture device that exempting from silt and its application method

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2000279078A (en) * 1999-03-31 2000-10-10 Akizo Ogawa Tool for holding lobworm or the like
CN100527953C (en) * 2005-11-25 2009-08-19 宁波大学 Perinereis aibihitensis Grube manual seedling-raising method
CN100420373C (en) * 2006-08-31 2008-09-24 黄自兴 Method for cultivating Tylorrhynchus heter Ochatetus

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103563861A (en) * 2013-11-07 2014-02-12 东营振宇水产资源开发有限公司 Perinereis aibuhitensis totally-artificial breeding technology
CN103583439A (en) * 2013-11-20 2014-02-19 文登市水产技术推广站 Method for improving egg washing efficiency of bay scallops
CN103636569A (en) * 2013-11-27 2014-03-19 安徽省郎溪县水产管理站 Method for cultivating initial feed of Pseudorasbora parva
CN103636569B (en) * 2013-11-27 2016-07-20 安徽省郎溪县水产管理站 A kind of breeding method of pseudorasbora parva open-mouthed bait
CN103651273A (en) * 2013-12-30 2014-03-26 盐城工学院 Perinereis aibuhitensis accumulated-water-free saturated wet soil cultivation method
CN106577589A (en) * 2016-12-09 2017-04-26 国家海洋局北海环境监测中心 A clam worm trapping apparatus and a method of trapping clam worms through the apparatus
CN109090050A (en) * 2018-08-29 2018-12-28 中国水产科学研究院黄海水产研究所 A kind of nereis culture device that exempting from silt and its application method

Also Published As

Publication number Publication date
CN101622974B (en) 2011-05-18

Similar Documents

Publication Publication Date Title
CN100403884C (en) Method for breeding river crab
CN104823883A (en) Macrobranchium rosenbergii larvae breeding method
CN102232362B (en) Biological cultivation method of sea horse
CN101411311B (en) Method for producing fingerling of Trachidermus fasciatus
CN103120140B (en) Scallop cultivation method
CN103404462B (en) Method for cultivating fries of coilia ectenes in soil pond
CN103798166B (en) The indoor extensive artificial breeding method of a kind of the South China coastal Hong Kong oyster
CN102106326B (en) Method for three-dimensional and artificial seedlings cultivation of perinereis aibuhitensis
CN101622974B (en) Soilless Nereid larva breeding method
CN102870722B (en) Large scale larva breeding method for navodon septentrionalis
CN102687695A (en) Yellow seahorse seedling artificial cultivating and farming method
CN101940182A (en) Manual grow seedlings method of Rapana venosa spat
CN105360030A (en) Earth pond artificial propagation and breeding integrated technology for crayfishes
CN103125415A (en) Artificial breeding method of tapes dorsatus
CN103563861A (en) Perinereis aibuhitensis totally-artificial breeding technology
CN103314895A (en) Artificial breeding method for parapenaeopsis hardwickii
CN100355337C (en) Artificial hybridization and breeding method for male Paralichthys dentatus and female summer flounder
CN103355224A (en) Novel stichopus japonicus fry breeding method
CN100370896C (en) Artificial breeding method for semi-smooth tongue sole
CN109122443B (en) Australia freshwater lobster circulating water egg hatching system and seedling raising method
CN104642089B (en) A kind of polyculture method of fragrant plant mentioned in ancient texts and grouper
CN1167334C (en) Method for overwintering and intermidiate breeding clam fry
CN101622973A (en) Soilless Nereid breeding method
CN110786277B (en) Artificial breeding and field culture method of Orychophragmus wartius
CN110810291B (en) Breeding method for mixed culture of golden lobsters and sea grapes

Legal Events

Date Code Title Description
PB01 Publication
C06 Publication
SE01 Entry into force of request for substantive examination
C10 Entry into substantive examination
GR01 Patent grant
C14 Grant of patent or utility model
EE01 Entry into force of recordation of patent licensing contract

Assignee: Zhanjiang Newpro Foods Co., Ltd.

Assignor: Guangdong Ocean University

Contract record no.: 2012440000030

Denomination of invention: Soilless Nereid larva breeding method

Granted publication date: 20110518

License type: Exclusive License

Open date: 20100113

Record date: 20120130

CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20110518

Termination date: 20120609

C17 Cessation of patent right