CN100527953C - Perinereis aibihitensis Grube manual seedling-raising method - Google Patents
Perinereis aibihitensis Grube manual seedling-raising method Download PDFInfo
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- CN100527953C CN100527953C CNB2005100617267A CN200510061726A CN100527953C CN 100527953 C CN100527953 C CN 100527953C CN B2005100617267 A CNB2005100617267 A CN B2005100617267A CN 200510061726 A CN200510061726 A CN 200510061726A CN 100527953 C CN100527953 C CN 100527953C
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Abstract
The invention discloses a method for seedling double-prong clam worm artificially, comprising the following steps: (1) parent breeding in indoor cement pool; (2) bailing out clam worm of different sex from suspending group; (3) hatching and laying eggs and breeding larval; (4) emergence of seedlings by means of sand flushing crashing; and the invention is characterized in that it applies light-control induction and animal substitute bait for accelerating parent in step (1), and laying 1-2 cm sterilized sand of 80-100 order in breeding pool from the fourth parapodia larval phase in step (3), and increasing substitute bait amount, which can increase the survival rate of double-prong clam worm to over 85%, increase the synchronizing mature rate to above 35%, reduce the damage rate of larval to below 5%, and avoid the problem of confinement for clam worm larval breeding because of shortage of breeding of single cell algae.
Description
Technical field
The present invention relates to a kind of cultural method of perinereis aibihitensis Grube, especially relate to a kind of method of perinereis aibihitensis Grube manual seedling-raising.
Background technology
The blue or green worm of perinereis aibihitensis Grube (Perinereis aibihitensis Grube) popular name, extra large centipede, extra large leech belong to Annelida, polychaeta, and migration crinosity order, Nereidae is enclosed the economic aquatic/marine animals of Nereis.In the climax band silt beach, coastal widely distributed in mainly living in China, and it is rapid, nutritious to grow, and measures according to one's analysis, and amino acid content accounts for 52.62% of dry weight; Crude fat content accounts for 23.24% of dry weight; Heat is up to the 4494-5578 card.The perinereis aibihitensis Grube main application has: one can be used as high-quality bait and baits such as fish, shrimp, crab, is called as " omnipotent bait "; It two contains rich in protein, delicious flavour, and the coastal and Southeast Asian countries resident of Japan has the custom of eating, and as delicious food, is the cool dish of emerging hot topic on the external dining table with it; It three is rich in eicosapentaenoic acid, is the desirable feed addictive of aquaculture, the effect that aquatic livestocks such as mud crab, prawn are had accelerating and improve fish brood amount; The medical value that its having ideals, morality, culture, and discipline is certain has tonifying yin void, antiasthmatic-antitussive effect, and its extract has been used to the clinical treatment of cancer, has exploitation and is worth; Its five coelomic fluid is a kind of natural insecticide (NTX), and this insecticides plays an important role in agricultural insect management so far, especially insect is had severe toxicity, and harmless to the mankind.Along with the development of mariculture, clam worm is had a sudden rise in social status, and the people claims omnipotent bait, is one of maximum clam worm kind of China's export volume.Japan is used for trip from a large amount of import clam worms of Korea S and China and angles industry and extract inclusion every year.China organizes the outlet of clam worm from the eighties, only nineteen eighty-two outlet Japan reaches the 80-100 ton, the millions of dollar of earning foreign exchange.But Korea S's resource decline in recent years sharply, and export volume sharply descends, and the wild naturally clam worm on ground such as the Jiangsu of China, Shandong is also exhausted day by day.According to Japanese market investigation in 99 years, the price of perinereis aibihitensis Grube (blue or green worm) reached 30 dollars/kg; Multiple tooth price of enclosing clam worm (red worm) is 100 dollars/kg.So people begin to attempt carrying out propagating artificially of clam worm in recent years, about the scholar to the maturation of clam worm and quicken to lay eggs, ecology, the history of life, the group of breed and perinereis aibihitensis Grube appear in one's mind resemble, aspects such as artificial breeding, artificial increasing cultivation have all done corresponding research and have had certain paper and report.And aspect propagating artificially, also carried out test preferably, and reach 1500 mu as Zhejiang Cixi City cultured area in 1998, take extensive style to culture, per mu yield reaches more than the 25Kg, and the input and output ratio is more than 1: 6, the benefit highly significant.But people find that by research and test back an important factor propagating perinereis aibihitensis Grube artificially depends on the seedling of the perinereis aibihitensis Grube that whether can provide sufficient.Most of kinds of Nereidae can become heteronereis after sexual maturity, and the life of dwelling the end of from changes into and swimming, and behind the certain hour that moves about fast, the row of laying eggs is smart.When perinereis aibihitensis Grube reaches sexual maturity in process of growth, can complete metamorphosis become to float on and do not stop the heteronereis that moves about in the water, in time fish for ripe heteronereis and can carry out the seedling that artificial breeding obtains perinereis aibihitensis Grube.But it is found that in practice the method for this acquisition perinereis aibihitensis Grube seedling is subjected to the influence in season bigger, and the not anti-long-distance transport of heteronereis, dried up after very easily miscarriage, death; And by hand digging catch that marked change has taken place the bodily form but also not the clam worm parent of come-up take back indoor supporting temporarily and obtain heteronereis and also have certain degree of difficulty; can not obtain the clam worm parent of q.s, thereby the clam worm seedling of the needed q.s of large-scale cultivation can't be provided.Rapid minimizing along with wild naturally clam worm resource; people begin to attempt adopting the method for artificial culture perinereis aibihitensis Grube parent to obtain the perinereis aibihitensis Grube seedling; but in concrete practice, find; mainly there is the asynchronous maturation of parent in the method that adopts artificial culture perinereis aibihitensis Grube parent to obtain the perinereis aibihitensis Grube seedling; cause the heteronereis lazy weight; can not carry out the demand that large-scale artificial is grown seedlings and satisfied breeding production; higher disability rate in this external perinereis aibihitensis Grube seedling culture and the process of emerging has also significantly reduced the quantity of the perinereis aibihitensis Grube seedling that can be used for culturing, and has therefore restricted the scale artificial culture of perinereis aibihitensis Grube.
Summary of the invention
Technical problem to be solved by this invention is the method that a kind of perinereis aibihitensis Grube manual seedling-raising is provided at above-mentioned prior art present situation; can induce the clam worm parent ripe synchronously effectively; reduce the disability rate of perinereis aibihitensis Grube seedling, thereby the seedling of abundance is provided for the scale artificial culture of perinereis aibihitensis Grube.
The present invention solves the problems of the technologies described above the technical scheme that is adopted: a kind of method of perinereis aibihitensis Grube manual seedling-raising may further comprise the steps: (1) is carried out parent and is supported temporarily in indoor cement pit; (2) fish for the floating heteronereis of group; (3) lay eggs, hatching and larval culture; (4) catch up with the method for seedling to emerge with sand washing; The method that adopts adjusting light induction and animal to substitute the bait reinforcement in step (1) is carried out the parent accelerating, regulate method that light induction carries out the parent accelerating and be to adopt in the indoor cultivation process being provided with in raising chamber that white transparent grp tile roof increases natural lighting and Continuous irradiation is carried out with a plurality of 200W incandescent lamps or 40W fluorescent lamp in the dark back of the colour of sky, illuminance is 200～300 μ mol/m
2S, illumination continuous time is 13～15 hours; The method that animal substitutes bait reinforcement carrying out parent accelerating is day to change water 100%, does and reveals 1 hour, and filter at least a elder generation in spirulina powder, fish meal or the soybean cake powder this moment with 200 orders～100 eye mesh screens, press 0.5g/m again
2～1g/m
2The amount ratio of day is thrown something and fed; Hatching in step (3) is laid eggs and larval culture can be selected following dual mode: the step of mode one is as follows: a, in 3: 1 ratios the heteronereis of male and female perinereis aibihitensis Grube is put into net cage and lay eggs; B, will arrange heteronereis smart, after laying eggs and remove the former pond hatching in back fertilized egg together with net cage; C, larval culture; And the wheel of the load in the larval culture process turns over the pond to nursery pond the phase; The step of mode two is as follows: a, in 3: 1 ratios the heteronereis of male and female perinereis aibihitensis Grube is put into drum and lay eggs; B, fertilized egg is collected the back with 300 purpose nylon mesh tulle casees use filtering sea to wash ovum; C, the fertilized egg of the too much seminal fluid of flush away is put into pond hatching and carried out larval culture; In step (3), the water in the nursery pond filters through husky filter of secondary and 200 orders, and full pond adds water 50～60cm, and water temperature is 25～33 ℃, and salinity is 20～26, and the pH value is 8.1～8.6, and dissolved oxygen adds disodium EDTA 5 * 10 greater than 4mg/L, inflation
-63 have just saved the nectocaete phase, add water 5cm～10cm every day, until filling it up with, the 4th firm joint nectocaete phase began, change water 30cm every day, after nectocaete crawls to bore sand, water level is reduced to 30cm, change water every day 1 time, change more than 95%, the 3rd just the joint nectocaete after date phase begin to throw something and feed at least a in whip chrysophyceae, Chaetoceros, Nannochloropsis oculata, the flat algae, since the 4th just joint nectocaete phase laying depth in cultivating the pond is the fine sand of 80～100 purposes behind scalding of 1～2cm, and increase and throw at least a in spirulina powder, fish meal or the soybean cake powder.
Compared with prior art, the invention has the advantages that by increasing intensity of illumination and prolonging light application time and carry out light induction and the animal of throwing something and feeding and substitute the method that bait strengthens and carry out the parent accelerating, can effectively improve perinereis aibihitensis Grube parent survival rate and synchronous maturing rate, parent survival rate of the present invention can reach more than 85%, and maturing rate reaches more than 35% synchronously; Since the 4th just joint nectocaete phase laying depth in cultivating the pond is the fine sand of 80～100 purposes behind scalding of 1～2cm, can be when adopting sand washing to catch up with the method for seedling to emerge the disability rate of perinereis aibihitensis Grube seedling be reduced to below 5%; Turn over the pond wheel phase in load and can avoid damage clam worm parapodum bristle; Substitute the feeding volume that bait can reduce single-cell algae and increase to throw, avoid restricting the incubation rate of perinereis aibihitensis Grube, thereby the seedling of abundance is provided for the scale artificial culture of perinereis aibihitensis Grube because of the deficiency of single-cell algae cultivation amount.
Description of drawings
The rule that Fig. 1 occurs for the indoor culture heteronereis
Fig. 2 is the rule that heteronereis occurs in the indoor culture one day
Fig. 3 is different illumination conditions and the relation of cultivating water temperature
Fig. 4 is the influence of illumination condition to heteronereis quantity
Embodiment
Embodiment describes in further detail the present invention below in conjunction with accompanying drawing.
First open the factory of growing seedlings of Bioisystech Co., Ltd in March, 2005 in Song Ao Ningbo, Fenghua, Zhejiang and carry out the artificial breeding of perinereis aibihitensis Grube, buy perinereis aibihitensis Grube parent 150kg from the Wenling, Zhejiang, the 2.02g that average weight is female, male 1.85g, 5 cement pit (8m * 4m * 1.2m) cultivate in raising chamber.
Embodiment one: raising chamber is provided with white transparent grp tile roof, indoor a plurality of 200W incandescent lamps is set, and the illuminance that incandescent lamp produced is 300 μ mol/m
2S, fine natural lighting, the cloudy day adds artificial light source, and the continuous illumination time is 13 hours, changes water every day, and quantity of exchanged water 100% is done and was revealed 1 hour, presses 0.75g/m this moment
2Day the amount ratio fish meal of throwing something and feeding, checked cultivation pond parent every 1 hour in the floating peak period of group, find heteronereis, pick up with diddle-net immediately, in 3: 1 ratios the heteronereis of the male and female perinereis aibihitensis Grube that picks up is put into the white Plastic Drum of 50L, little inflation, allow its natural spawning, put 100 for every barrel, after 2 hours heteronereis pulled out, with 300 purpose bolting silk nets with ovum collecting, wash ovum repeatedly with filtering sea rapidly, (5m * 8m * 1.2m) hatching cultivates in nursery pond, and the water in the nursery pond must filter through husky filter of secondary and 200 orders to put into the cement pit of growing seedlings then, full pond adds water 60cm, water temperature is 28 ℃, and salinity is 23, and the pH value is 8.3, dissolved oxygen is 4mg/L, inflation adds disodium EDTA 5 * 10
-63 have just saved the nectocaete phase, add water 5cm every day, until filling it up with, the 4th firm joint nectocaete phase began, change water 30cm every day, and throw sand in cultivating the pond, laying depth is the fine sand of 100 purposes behind scalding of 1cm, after nectocaete crawls to bore sand, water level is reduced to 30cm, changes water every day 1 time, changes more than 95%, the 3rd just joint nectocaete after date phase begin to throw something and feed whip chrysophyceae and Chaetoceros, since the 4th just the joint nectocaete phase increase the throwing soybean cake powder, soybean cake powder is earlier with the filtration of 200 eye mesh screens, feeding volume 0.5g/m
2, change to do when revealing behind the water and throw something and feed; Catch up with the method for seedling to emerge with sand washing, the vanning back adopts the method for low temperature transportation to transport the perinereis aibihitensis Grube seedling to each plant.
Embodiment two: raising chamber is provided with white transparent grp tile roof, indoor a plurality of 40W fluorescent lamps is set, and the illuminance that fluorescent lamp produced is 200 μ mol/m
2S, fine natural lighting, the cloudy day adds artificial light source, and illumination continuous time is 15 hours, changes water every day, and quantity of exchanged water 100% is done and was revealed 1 hour, presses 1.0g/m this moment
2Day the amount ratio fish meal of throwing something and feeding, checked cultivation pond parent every 1 hour in the floating peak period of group, find heteronereis, pick up with diddle-net immediately, (net cage of 5m * 8m * 1.2m) is laid eggs in 3: 1 ratios the heteronereis of male and female perinereis aibihitensis Grube to be put into the cement pit of growing seedlings, water in the nursery pond must filter through husky filter of secondary and 200 orders, and full pond adds water 50cm, and water temperature is 25 ℃, salinity is 20, the pH value is 8.1, and dissolved oxygen is 4mg/L, inflation, adds disodium EDTA 5 * 10
-6To arrange smart, heteronereis after laying eggs is removed back former pond hatching fertilized egg together with net cage and is cultivated in nursery pond, the load wheel phase in the larval culture process is turned over the pond, 3 have just saved the nectocaete phase, add water 8cm every day, until filling it up with, the 4th firm joint nectocaete phase began, change water 30cm every day, and in cultivating the pond, throw sand, laying depth is the fine sand of 80 purposes behind scalding of 2cm, and after nectocaete crawled to bore sand, water level was reduced to 30cm, change water every day 1 time, change more than 95%, the 3rd firm joint nectocaete after date phase began the Nannochloropsis oculata of throwing something and feeding, and just the joint nectocaete phase has increased the throwing fish meal since the 4th, fish meal filters with 100 eye mesh screens earlier, feeding volume 1g/m
2, change to do when revealing behind the water and throw something and feed; Catch up with the method for seedling to emerge with sand washing, the vanning back adopts the method for low temperature transportation to transport the perinereis aibihitensis Grube seedling to each plant.
Embodiment three: raising chamber is provided with white transparent grp tile roof, indoor a plurality of 200W incandescent lamps is set, and the illuminance that incandescent lamp produced is 250 μ mol/m
2S, fine natural lighting, the cloudy day adds artificial light source, and illumination continuous time is 14 hours, changes water every day, and quantity of exchanged water 100% is done and was revealed 1 hour, presses 0.5g/m this moment
2Day the amount ratio fish meal of throwing something and feeding, checked cultivation pond parent every 1 hour in the floating peak period of group, find heteronereis, pick up with diddle-net immediately, in 3: 1 ratios the heteronereis of the male and female perinereis aibihitensis Grube that picks up is put into the white Plastic Drum of 50L, little inflation, allow its natural spawning, put 100 for every barrel, after 2 hours heteronereis pulled out, with 300 purpose bolting silk nets with ovum collecting, wash ovum repeatedly with filtering sea rapidly, (5m * 8m * 1.2m) hatching cultivates in nursery pond, and the water in the nursery pond must filter through husky filter of secondary and 200 orders to put into the cement pit of growing seedlings then, full pond adds water 55cm, water temperature is 33 ℃, and salinity is 26, and the pH value is 8.6, dissolved oxygen is 4mg/L, inflation adds disodium EDTA 5 * 10
-63 have just saved the nectocaete phase, add water 10cm every day, until filling it up with, the 4th firm joint nectocaete phase began, change water 30cm every day, and throw sand in cultivating the pond, laying depth is the fine sand of 90 purposes behind scalding of 1.5cm, after nectocaete crawls to bore sand, water level is reduced to 30cm, changes water every day 1 time, changes more than 95%, the 3rd just joint nectocaete after date phase begin to throw something and feed flat algae and Chaetoceros, since the 4th just the joint nectocaete phase increase the throwing spirulina powder, spirulina powder is earlier with the filtration of 100 eye mesh screens, feeding volume 0.75g/m
2, change to do when revealing behind the water and throw something and feed; Catch up with the method for seedling to emerge with sand washing, the vanning back adopts the method for low temperature transportation to transport the perinereis aibihitensis Grube seedling to each plant.
Obviously 4 peaks appear promptly from Fig. 1 clam worm group on visible June 17 to August 8 is floating: 18～June 22 June (lunar calendar 13～16); 29～July 5 June (23～29); 13～July 20 July (the eighth day of lunar month～15); 26～August 4 July (21～30), the group shows as every month and 2 minor peaks occur in the floating cycle, each group is floating all to be occurred at little morning and evening tides the next day or the same day later, to maximum morning and evening tides end, continue 5～9 days, all arrived preceding 2 ~ 4 days at big morning and evening tides in the top, promptly between crescent and the full moon, it is floating to belong to typical first quarter moon facies pattern group; As can be seen from Table 1, obtain 15295 of heteronereises altogether to 8 the end of month, heteronereis reaches 1636 in one day the most for a long time, the parent survival rate reaches 85%, synchronous maturing rate reaches 35%, 13 cement pit and obtains 7.264 hundred million on ovum altogether, average incubation rate 72.35%, obtain 3 and just saved 52,556 ten thousand of parapodums, the larva incubation rate is 48.99%～91.97%; Bring out 2149.20 ten thousand of the perinereis aibihitensis Grube seedlings of the long 19.14mm of average body altogether, average 5.166 ten thousand/m
2, gross weight 153kg.
Table 1
In the cultivation of growing seedlings, we have carried out the check experiment of different illumination conditions to the floating influence of group: the condition of cultivation group is identical with embodiment one; Contrast 1 group and adopt white transparent grp tile roof, natural lighting, intense light irradiation 300 μ mol/m
2S; Contrast 2 groups and adopt smoked glass steel tile roofs, natural lighting, intense light irradiation 100 μ mol/m
2S; Contrast 3 groups and adopt grey transparent grp tile roofs, the dark back of natural lighting+colour of sky to use the 200W lamp lighting continuously, intense light irradiation 100 μ mol/m with 1 hour dark, the bright interval of 20min
2S; Contrast 4 groups and adopt white transparent grp tile roof to add black sunshade net, intense light irradiation 50 μ mol/m
2S.The variations in temperature in each pond as shown in Figure 3 and other conditions all with.Throw in each 500 of parents for every group, the result of the test among Fig. 4 shows to increase intense light irradiation intensity and prolong light application time can obviously induce clam worm to concentrate the group floating.Illumination is lower than 50 μ mol/m
24 groups of the contrasts of s to 1 heteronereis of off-test yet and do not occur; Contrast 1 group and 248 heteronereises occur, and cultivation group and to contrast 1 group of intense light irradiation all be 300 μ mol/m
2S, but the cultivation group prolongs light application time with artificial light source again, 407 heteronereises occur, and comparison exceeds 1.64 times according to 1 group; Though contrasting 2 groups and 3 groups of intense light irradiations of contrast all is 100 μ mol/m
2S uses (20min) illumination of artificial light source gap short time again but contrast 3 groups, and the heteronereis quantity difference that the result occurs is respectively 99 and 170.
Claims (1)
1, a kind of method of perinereis aibihitensis Grube manual seedling-raising may further comprise the steps: (1) is carried out parent and is cultivated in indoor cement pit; (2) fish for the floating heteronereis of group; (3) lay eggs the hatching and larval culture; (4) catch up with the method for seedling to emerge with sand washing; It is characterized in that in step (1) adopting and regulate light induction and animal and substitute the method that bait strengthens and carry out the parent accelerating, regulate method that light induction carries out the parent accelerating and be to adopt in the indoor cultivation process being provided with in raising chamber that white transparent grp tile roof increases natural lighting and Continuous irradiation is carried out with a plurality of 200W incandescent lamps or 40W fluorescent lamp in the dark back of the colour of sky, illuminance is 200～300 μ mol/m
2S, illumination continuous time is 13～15 hours; The method that animal substitutes bait reinforcement carrying out parent accelerating is day to change water 100%, does and reveals 1 hour, and filter at least a elder generation in spirulina powder, fish meal or the soybean cake powder this moment with 200 orders～100 eye mesh screens, press 0.5g/m again
2～1g/m
2The amount ratio of day is thrown something and fed; Hatching in step (3) is laid eggs and larval culture can be selected following dual mode: the step of mode one is as follows: a, in 3: 1 ratios the heteronereis of male and female perinereis aibihitensis Grube is put into net cage and lay eggs; B, will arrange heteronereis smart, after laying eggs and remove the former pond hatching in back fertilized egg together with net cage; C, larval culture; And the wheel of the load in the larval culture process turns over the pond to nursery pond the phase; The step of mode two is as follows: a, in 3: 1 ratios the heteronereis of male and female perinereis aibihitensis Grube is put into drum and lay eggs; B, fertilized egg is collected the back with 300 purpose nylon mesh tulle casees use filtering sea to wash ovum; C, the fertilized egg of the too much seminal fluid of flush away is put into pond hatching and carried out larval culture; In step (3), the water in the nursery pond filters through husky filter of secondary and 200 orders, and full pond adds water 50～60cm, and water temperature is 25～33 ℃, and salinity is 20～26, and the pH value is 8.1～8.6, and dissolved oxygen adds disodium EDTA 5 * 10 greater than 4mg/L, inflation
-63 have just saved the nectocaete phase, add water 5cm～10cm every day, until filling it up with, the 4th firm joint nectocaete phase began, change water 30cm every day, after nectocaete crawls to bore sand, water level is reduced to 30cm, change water every day 1 time, change more than 95%, the 3rd just the joint nectocaete after date phase begin to throw something and feed at least a in whip chrysophyceae, Chaetoceros, Nannochloropsis oculata, the flat algae, since the 4th just joint nectocaete phase laying depth in cultivating the pond is the fine sand of 80～100 purposes behind scalding of 1～2cm, and increase and throw at least a in spirulina powder, fish meal or the soybean cake powder.
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Families Citing this family (13)
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| CN100420373C (en) * | 2006-08-31 | 2008-09-24 | 黄自兴 | Method for cultivating Tylorrhynchus heter Ochatetus |
| CN100502646C (en) * | 2007-05-28 | 2009-06-24 | 大连水产学院 | Method for indoors artificial and intensive culture of clamworm |
| CN101755710B (en) * | 2008-12-23 | 2014-01-15 | 大连海洋大学 | Industrial artificial seedling rearing method of marrhysa sanguinea |
| CN101564023B (en) * | 2009-04-28 | 2011-12-07 | 大连水产学院 | Middle breeding method for large-specification seedlings of perineris aibihitensis grube |
| CN101622974B (en) * | 2009-06-09 | 2011-05-18 | 广东海洋大学 | Soilless Nereid larva breeding method |
| CN103053509A (en) * | 2012-12-10 | 2013-04-24 | 中国科学院海洋研究所 | Method and device for removing harmful biology including copepod and the like in artificial breeding water body |
| CN103004701B (en) * | 2012-12-27 | 2014-07-30 | 山东省海洋生物研究院 | Indoor raising method for perinereis aibuhitensis by circulating water |
| CN103283689B (en) * | 2013-05-23 | 2014-11-12 | 山东省海水养殖研究所 | Propagation control method for indoor cultivation of Perinereis aibuhitensis |
| CN103563861B (en) * | 2013-11-07 | 2014-12-10 | 东营振宇水产资源开发有限公司 | Perinereis aibuhitensis totally-artificial breeding technology |
| CN105994052B (en) * | 2016-05-30 | 2018-11-06 | 珠海市斗门区河口渔业研究所 | A kind of artificial breeding of standing grain worm and intensive cultural method |
| CN106614161B (en) * | 2016-12-31 | 2017-10-27 | 中国水产科学研究院黄海水产研究所 | A kind of clam worm forms method |
| CN111011275A (en) * | 2020-01-19 | 2020-04-17 | 福建省淡水水产研究所 | Artificial spawning induction and hatching method of rhynchophorus wartius |
| CN112450132B (en) * | 2020-11-24 | 2022-06-28 | 阳江职业技术学院 | Method for artificially hastening parturition and hatching of sipunculus warteri |
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