CN103270982B - Manual offspring seed cultivating method for blepharipoda liberata - Google Patents
Manual offspring seed cultivating method for blepharipoda liberata Download PDFInfo
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- CN103270982B CN103270982B CN201310191700.9A CN201310191700A CN103270982B CN 103270982 B CN103270982 B CN 103270982B CN 201310191700 A CN201310191700 A CN 201310191700A CN 103270982 B CN103270982 B CN 103270982B
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- 238000000034 method Methods 0.000 title claims abstract description 12
- 241000472286 Blepharipoda liberata Species 0.000 title 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 50
- 210000004709 eyebrow Anatomy 0.000 claims description 29
- 238000009395 breeding Methods 0.000 claims description 10
- 230000003203 everyday effect Effects 0.000 claims description 9
- 239000011521 glass Substances 0.000 claims description 9
- 239000004576 sand Substances 0.000 claims description 9
- 230000034004 oogenesis Effects 0.000 claims description 7
- 239000012535 impurity Substances 0.000 claims description 6
- 239000012286 potassium permanganate Substances 0.000 claims description 6
- 239000013535 sea water Substances 0.000 claims description 6
- 239000004575 stone Substances 0.000 claims description 6
- 230000012447 hatching Effects 0.000 claims description 4
- 241000251468 Actinopterygii Species 0.000 claims description 3
- 241000894006 Bacteria Species 0.000 claims description 3
- 241001247197 Cephalocarida Species 0.000 claims description 3
- 241000227752 Chaetoceros Species 0.000 claims description 3
- 241000206751 Chrysophyceae Species 0.000 claims description 3
- 241000195493 Cryptophyta Species 0.000 claims description 3
- 241000238557 Decapoda Species 0.000 claims description 3
- 230000002159 abnormal effect Effects 0.000 claims description 3
- 239000004568 cement Substances 0.000 claims description 3
- 230000036541 health Effects 0.000 claims description 3
- 238000005286 illumination Methods 0.000 claims description 3
- 230000001418 larval effect Effects 0.000 claims description 3
- 230000009571 larval growth Effects 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 3
- 230000035800 maturation Effects 0.000 claims description 3
- 235000013372 meat Nutrition 0.000 claims description 3
- 238000006213 oxygenation reaction Methods 0.000 claims description 3
- 239000006041 probiotic Substances 0.000 claims description 3
- 235000018291 probiotics Nutrition 0.000 claims description 3
- 230000001105 regulatory effect Effects 0.000 claims description 3
- 238000009287 sand filtration Methods 0.000 claims description 3
- 230000008859 change Effects 0.000 claims description 2
- 239000003651 drinking water Substances 0.000 claims description 2
- 235000020188 drinking water Nutrition 0.000 claims description 2
- 230000008901 benefit Effects 0.000 abstract description 8
- 235000013601 eggs Nutrition 0.000 abstract description 7
- 238000009360 aquaculture Methods 0.000 abstract description 2
- 244000144974 aquaculture Species 0.000 abstract description 2
- 241000766710 Blepharipoda Species 0.000 abstract 4
- 238000011534 incubation Methods 0.000 abstract 1
- 230000001488 breeding effect Effects 0.000 description 3
- 230000008569 process Effects 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- 230000009182 swimming Effects 0.000 description 2
- 230000003115 biocidal effect Effects 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- -1 density is 0.5 ∕ m2 Substances 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 210000004907 gland Anatomy 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000000384 rearing effect Effects 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 235000014102 seafood Nutrition 0.000 description 1
- 230000001568 sexual effect Effects 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Farming Of Fish And Shellfish (AREA)
Abstract
The invention discloses a manual offspring seed cultivating method for blepharipoda liberate, and relates to the technical field of aquaculture, in particular to the manual offspring seed cultivating method for the blepharipoda liberate. The manual offspring seed cultivating method for the blepharipoda liberate is characterized by comprising the following steps of (1) cultivation of parent bodies, (2) incubation of fertilized eggs, (3) cultivation of larvas and (4) leaving of offspring seeds from pools. The manual offspring seed cultivating method for the blepharipoda liberate has the advantages of being free of limitation of natural conditions, high in yield and remarkable in benefit.
Description
Technical field
The present invention relates to technical field of aquaculture, particularly belong to a kind of artificial seed's breeding method of liberating eyebrow foot crab.
Background technology
Liberation eyebrow foot crab is the different tail class biology on Marine shoal as one way of life, and its existence quantity is very limited.Put on the dining table because liberation eyebrow foot crab is used as seafood speciality end in recent years, consumer increases greatly to the demand of liberation eyebrow foot crab, thereby has caused thereupon increasing of its amount of excavating; In addition the discharge of coastal pollutant has destroyed its living environment, has had a strong impact on the reproductive survival rate of liberation eyebrow foot crab; So the existence quantity of liberation eyebrow foot crab is always in downward trend.At present, country is Development of Marine economy just energetically, as marine industry, these precious marine organisms of exploitation liberation eyebrow foot crab, the marine product of more safe green can be provided for people, better protein source is provided, there are huge economic benefit and ecological benefits, but will accomplish that this point just must solve seed rearing problem.For natural propagation, the method that artificial seed cultivates has that survival rate is high, Larval quality good, be not subject to the plurality of advantages such as natural conditions restriction, and nowadays about the artificial breeding technique of liberation eyebrow foot crab at marine industry always in space state.
Summary of the invention
The object of the present invention is to provide a kind of liberation eyebrow foot crab artificial seed breeding method, improve larva survive ratio to reach, increase liberation eyebrow foot crab total resources, increase economic efficiency and the object of ecological benefits.
Liberation eyebrow foot crab artificial seed breeding method provided by the present invention, is characterized in that, comprises the steps:
1) parents culture:
Step 1-1, the sea sand impurity afterwash such as removing chad, shell that sieves, with concentration be 20 × 10
-6disinfecting solution of potassium permanganate after, be layered in indoor light water mud sump or glass jar pond, thickness is 15～20 ㎝;
Step 1-2, manually cultivates liberation eyebrow foot crab adult in pond as parent;
Step 1-3, adopts fresh small fish, shrimp and clam meat, and, chopping clean with seawater, as the bait of throwing in;
Step 1-4, cultivation water is drained into residue 2～5 ㎝ degree of depth, now liberating eyebrow foot crab can expose feeler and mouthpart, and the overwhelming majority of health is still embedded in sand, open the water valve of cultivating pond one side, regulating pondage makes the glassware for drinking water flowing out have impulsive force, in the time that pond water flow gets up, bait is cast, and throws something and feeds every day once;
Step 1-5, keeps cultivating with coolant-temperature gage between 4 DEG C～10 DEG C in pond, and salinity is 26 ‰～30 ‰, and pH value is 7.8～8.9, makes female body oogenesis;
2) incubating oosperm:
Step 2-1: the sea sand impurity afterwash such as removing chad, shell that sieves, with concentration be 20 × 10
-6disinfecting solution of potassium permanganate after, be layered in indoor light water mud sump or glass jar pond, thickness is 15-20cm, using this as hatchery;
Step 2-2: make water temperature remain on 10 DEG C～25 DEG C, salinity is 26 ‰～30 ‰, and pH value is 7.8～8.9, changes water every day once, quantity of exchanged water 90 ﹪, and pool inner water position remains on 30～50 ㎝;
Step 2-3: when after the female body oogenesis of maturation, carry out the hatching of fertilized egg in hatchery, threw something and fed once every 1～2 day, feeding method is identical with parents culture;
3) larval culture:
Step 3-1, selected depth is indoor cement pit or the glass jar tank of 1～1.5 m, and oxygenation facility is installed, and to make indoor illumination intensity be 200～1000lx;
Step 3-2 lays 60 object gas stones in pond, and density is 0.5 ∕ ㎡, gas stone by gas line from roof vertical hanging in pond; The young of collecting liberation eyebrow foot crab, carries out artificial culture;
Step 3-3, adopts through one-level sand filtration, salinity be 26 ‰～30 ‰ seawater as cultivating water, and make water temperature remain on 21～28 DEG C, and in water, add Chaetoceros or chrysophyceae, its algae density is respectively 1～30,000 cell/mL and 3～50,000 cell/mL; Change water 20～40 ﹪ every day, along with larval growth strengthens quantity of exchanged water gradually;
Step 3-4, adds probiotics EM bacterium liquid and improves water quality in water;
Step 3-5, with the artemia nauplii live body of just incubating as open-mouthed bait, from the young hatch that first day starts to throw something and feed until the young all abnormal be young crab;
4) seed goes out pond:
Because liberation eyebrow foot crab children crab is no longer sought the life of swimming, but lie prostrate at the bottom of cultivating pond, so while Chi Shui being drawn to 10～20 ㎝ degree of depth with 40 order net cages, operating personnel enter in pond, be that 120～80 object fishing nets pick up young crab gently by sieve screen apertures, and avoid moving too quickly damage seed as far as possible.
Liberation eyebrow foot crab in the annual breeding of natural world once, in annual solar calendar early March left and right, by liberation eyebrow foot crab parent at the indoor artificial culture that carries out, make its egg reproduction, at this moment consistent with the repoductive time of wild stocks on beach, the seed of cultivating can discharge in natural environment, repairs natural resources, also can be used for proceeding to propagate artificially.In addition, can suitable nurturing an environment be artificially set by the measure of temperature raising or cooling and make it all can reach the required reproduction condition of liberation eyebrow foot crab in each season, and then the breeding that makes to liberate eyebrow foot crab avoids being subject to the constraint of natural conditions; Therefore, have that the natural conditions of not being subject to restrictions, output are high, the good effect of remarkable benefit.
Embodiment
Be further described below in conjunction with specific embodiment.This embodiment is coastal at From Shandong Rizhao, early March carries out.
First, by the sea sand impurity afterwash such as removing chad, shell that sieves, with concentration be 20 × 10
-6disinfecting solution of potassium permanganate after, be layered in indoor light water mud sump or glass jar pond, thickness is 20 ㎝.Choose figure complete, be of moderate size, liberation eyebrow that energy is strong foot crab adult carries out artificial culture in pond as parent.After adopting the fresh meat seawater of small fish, shrimp and clam to clean, shred, as the bait of throwing in.
Cultivation Chi Shui is drained into the residue 3 ㎝ degree of depth, now liberating eyebrow foot crab can expose feeler and mouthpart, and the overwhelming majority of health is still embedded in sand, open the water valve of cultivating pond one side, regulating pondage makes the current that flow out have certain impulsive force, in the time that pond water flow gets up, bait is cast, throw something and feed once every day.And, keep pool inner water temperature at 6～8 DEG C, salinity is 27 ‰ left and right, pH value is 8.0～8.2, until female body oogenesis.
When after the female body oogenesis of maturation, in the indoor hatching of carrying out fertilized egg; The impurity afterwash such as removing chad, shell that equally sea sand sieved, with 20 × 10
-6after the potassium permanganate sterilization of concentration, be layered in indoor light water mud sump or glass jar pond, thickness is 20cm, using this as hatchery; Make water temperature remain on 15～20 DEG C, salinity is 27 ‰ left and right, and pH value is 8.0～8.2, changes water every day once, quantity of exchanged water 90 ﹪, and pool inner water position remains on 40 ㎝; In the hatching process of fertilized egg, within 1～2 day, throw something and feed once, feeding method is identical with parents culture, and not in this to go forth.
After incubating oosperm, collect the young of liberation eyebrow foot crab; Select cement pit or the glass jar tank of dark 1.4 meters of tank interior, oxygenation facility is installed, use air compressor as inflation power; And to make indoor illumination intensity be 200-1000lx; In pond, lay 60 object gas stones, density is 0.5 ∕ ㎡, gas stone by gas line from roof vertical hanging in pond, apart from 1 about ㎝ at the bottom of pond.
Adopt through one-level sand filtration, salinity be the seawater of 27 ‰ left and right as cultivating water, and make water temperature remain on 25～27 DEG C; In water, be added with Chaetoceros or chrysophyceae, its algae density is respectively 2.4 ten thousand cell/mL and 3.2 ten thousand cell/mL, changes water 30 ﹪ every day, along with larval growth strengthens quantity of exchanged water gradually; In water, timing is added probiotics EM bacterium liquid and is improved water quality, and whole cultivating process does not add any antibiotic or other chemicalss.
With the artemia nauplii live body of just incubating as open-mouthed bait, from the young hatch that first day starts to throw something and feed until the young all abnormal be young crab.
Because liberation eyebrow foot crab children crab is no longer sought the life of swimming, but at the bottom of Fu Yuchi, so when Chi Shui is drawn to the 12 ㎝ degree of depth by 40 object net cages, operating personnel enter in pond, be that 120 object fishing nets pick up young crab gently by sieve screen apertures, avoid moving too quickly damage seed as far as possible.
Adopt liberation eyebrow foot crab artificial seed breeding method provided by the present invention, if just start in the winter time the cultivation of parent, can adopt the device such as solar energy or electrical heating to improve and cultivate the temperature of water, make parent sexual gland grow in advance, lay eggs, and then make seed go out pond season in advance; In addition, also can by the parent of not laying eggs or the parent of oogenesis be placed in the raising chamber of refrigerated condition, make it cultivate temperature of water lower than natural temperature and be not less than 1 DEG C, postpone the season that can make to grow seedlings.Therefore artificial seed's breeding method has the plurality of advantages such as enforcement is flexible, success rate is high.
Claims (1)
1. a liberation eyebrow foot crab artificial seed breeding method, is characterized in that, comprises the steps:
1) parents culture:
Step 1-1, sea sand sieves and removes impurity afterwash, with concentration be 20 × 10
-6disinfecting solution of potassium permanganate after, be layered in indoor light water mud sump or glass jar pond, thickness is 15～20 ㎝;
Step 1-2, manually cultivates liberation eyebrow foot crab adult in pond as parent;
Step 1-3, adopts fresh small fish, shrimp and clam meat, and, chopping clean with seawater, as the bait of throwing in;
Step 1-4, cultivation water is drained into residue 2～5 ㎝ degree of depth, now liberating eyebrow foot crab can expose feeler and mouthpart, and the overwhelming majority of health is still embedded in sand, open the water valve of cultivating pond one side, regulating pondage makes the glassware for drinking water flowing out have impulsive force, in the time that pond water flow gets up, bait is cast, and throws something and feeds every day once;
Step 1-5, keeps cultivating with coolant-temperature gage between 4 DEG C～10 DEG C in pond, and salinity is 26 ‰～30 ‰, and pH value is 7.8～8.9, makes female body oogenesis;
2) incubating oosperm:
Step 2-1: sea sand sieves and removes impurity afterwash, with concentration be 20 × 10
-6disinfecting solution of potassium permanganate after, be layered in indoor light water mud sump or glass jar pond, thickness is 15-20cm, using this as hatchery;
Step 2-2: make water temperature remain on 10 DEG C～25 DEG C, salinity is 26 ‰～30 ‰, and pH value is 7.8～8.9, changes water every day once, quantity of exchanged water 90 ﹪, and pool inner water position remains on 30～50 ㎝;
Step 2-3: when after the female body oogenesis of maturation, carry out the hatching of fertilized egg in hatchery, threw something and fed once every 1～2 day, feeding method is identical with parents culture;
3) larval culture:
Step 3-1, selected depth is indoor cement pit or the glass jar tank of 1～1.5 m, and oxygenation facility is installed, and to make indoor illumination intensity be 200～1000lx;
Step 3-2 lays 60 object gas stones in pond, and density is 0.5 ∕ ㎡, gas stone by gas line from roof vertical hanging in pond; The young of collecting liberation eyebrow foot crab, carries out artificial culture;
Step 3-3, adopts through one-level sand filtration, salinity be 26 ‰～30 ‰ seawater as cultivating water, and make water temperature remain on 21～28 DEG C, and in water, add Chaetoceros or chrysophyceae, its algae density is respectively 1～30,000 cell/mL and 3～50,000 cell/mL; Change water 20%～40 ﹪ every day, within the scope of this, along with larval growth strengthens quantity of exchanged water gradually;
Step 3-4, adds probiotics EM bacterium liquid and improves water quality in water;
Step 3-5, with the artemia nauplii live body of just incubating as open-mouthed bait, from the young hatch that first day starts to throw something and feed until the young all abnormal be young crab;
4) seed goes out pond:
While Chi Shui being drawn to 10～20 ㎝ degree of depth with 40 order net cages, operating personnel enter in pond, are that 120～80 object fishing nets pick up young crab gently by sieve screen apertures, and avoid moving too quickly damage seed as far as possible.
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104621019B (en) * | 2015-01-27 | 2017-02-01 | 日照市海洋与渔业研究所 | Artificial culturing method for blepharipoda liberata |
| CN106212348A (en) * | 2016-09-19 | 2016-12-14 | 山东省海洋资源与环境研究院 | A kind of facility cultivating Concha Ostreae floating larva |
| CN108124804B (en) * | 2017-12-27 | 2020-08-25 | 日照市海洋与渔业研究所(日照市海域使用动态监视监测中心、日照市水生动物救护站) | Crab releasing method for liberating brow and feet |
| CN109619004A (en) * | 2019-01-25 | 2019-04-16 | 中国水产科学研究院东海水产研究所 | A kind of Eriocheir sinensis ovigerous crab indoor culture method |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2200386C1 (en) * | 2001-12-27 | 2003-03-20 | Федеральное Государственное Унитарное Предприятие Всероссийский научно-исследовательский институт рыбного хозяйства и океанографии | Method for reproduction in crustacean (king crab) |
| CN102106278A (en) * | 2010-12-30 | 2011-06-29 | 广东海洋大学 | Method for breeding marine crabs artificially |
| CN102613126A (en) * | 2012-04-26 | 2012-08-01 | 中国水产科学研究院黄海水产研究所 | High-efficient ecological seedling-raising method of blue crabs |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100924883B1 (en) * | 2009-03-20 | 2009-11-02 | 명노환 | Crab Form Method and Crab Form Device |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2200386C1 (en) * | 2001-12-27 | 2003-03-20 | Федеральное Государственное Унитарное Предприятие Всероссийский научно-исследовательский институт рыбного хозяйства и океанографии | Method for reproduction in crustacean (king crab) |
| CN102106278A (en) * | 2010-12-30 | 2011-06-29 | 广东海洋大学 | Method for breeding marine crabs artificially |
| CN102613126A (en) * | 2012-04-26 | 2012-08-01 | 中国水产科学研究院黄海水产研究所 | High-efficient ecological seedling-raising method of blue crabs |
Non-Patent Citations (2)
| Title |
|---|
| 中华绒螯蟹地下咸水工厂化育苗高产技术;刘月芬等;《水产科学》;20040331;第23卷(第3期);第26-28页 * |
| 刘月芬等.中华绒螯蟹地下咸水工厂化育苗高产技术.《水产科学》.2004,第23卷(第3期),第26-28页. |
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