CN111011275A - Artificial spawning induction and hatching method of rhynchophorus wartius - Google Patents
Artificial spawning induction and hatching method of rhynchophorus wartius Download PDFInfo
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/40—Culture of aquatic animals of annelids, e.g. lugworms or Eunice
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
- A01K61/17—Hatching, e.g. incubators
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
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- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention relates to an artificial induced spawning and hatching method of Orychophragmus wartius, which comprises the following steps: preparation before induction of labor, selection of parents, artificial insemination, egg washing and artificial incubation. The invention fully utilizes the reproductive biological characteristics of the Oryza sativa, artificially simulates the water area environmental conditions during natural reproduction, artificially assists induced spawning and innovatively optimizes the incubation method, solves the problems of the aquatic environment conditions of the Oryza sativa reproduction, the selection of the seed insects, the proportion of male and female parents, the fertilization time and the incubation technology, shortens the spawning time, improves the spawning amount, the fertilization rate and the incubation rate, realizes the large-scale artificial breeding of the Oryza sativa, lays a foundation for protecting germplasm resources of the Oryza sativa, developing proliferation and releasing and artificial breeding, and has important significance for the intensive development of the industry of the Oryza sativa in China.
Description
Technical Field
The invention belongs to the field of aquatic seedling breeding, relates to an artificial breeding technology of clamworm, and particularly relates to an artificial induced spawning and hatching method of rhynchophorus wartius.
Background
Wart Bombycis (Tylorrhynchus heterochaetus) Belongs to the ringOnychida (Annelida), Polychaeta (Polychaeta), Errantia (Errantia), Nereidinae (Nereidinae), Aristolochia (Aristolochia)Tylorrhynchus) Often called as standing insects and river skipper, usually inhabit coastal waters or river shoals and are mainly distributed in Zhejiang, Fujian, Guangdong and Guangxi. The polyose has fresh and tender meat and rich nutrients, and has the features of high protein, high fat, complete amino acid composition, rich essential amino acid content, etc. although it is very tough, it is called "Jiangshan cordyceps sinensis". The wart snout clamworm is used as both food and medicine, has the efficacy of detumescence and tonifying qi, is reputed by ancient and modern times, and is praised by Huangting Biao Shi Gao (having a sense of eating standing grain insects): the catfish of the autumn wind weever is beautiful in the United states, and the hilsa herring is also inferior to the other summer months, so that the catfish is delicious; the record of compendium shiita: the food can tonify spleen and stomach, promote hematogenesis, promote diuresis and promote urination; the "materia medica asking for the original" is also recorded: warm stomach and tonify qi.
In recent years, the kisnereis wartwort is gradually perceived by consumers slowly, ferocious in posture is unacceptable to people, but delicious taste and rich nutritional value enable people to feel radiant, so that the kisnereis wartwort is gradually regarded as an excellent seafood product which is not popular in the market and is deeply popular in the market. The rhynchophorus schlegelii has the obvious characteristics of strong time-saving property, low yield, high fishing cost and the like, has strict requirements on the growth environments such as climate, hydrology, soil and the like, and cannot survive after being slightly polluted. The natural population of the ps wartii is seriously declined under the influence of factors such as over fishing, water environment pollution and the like, and the yield per mu of the mudflat is reduced to 10-25 kg/year from 50-100 kg/year of the twentieth century. At present, wild rhynchophyllus is only found in individual areas of Guangdong Zhongshan Gonsbay, Zhuhai fighter and Panyun, mudflat areas of Fujian Min, Fuan and other places where salt and fresh water is located, paddy fields and other places, the yield is extremely low, the market supply is not sufficient, and the wholesale price of the rhynchophyllus in 2017 and 2019 is as high as 300 yuan/kg. Therefore, the development of artificial induced spawning, breeding and breeding of the rhynchophorus wartius becomes a social demand.
The research on the rhynchophorus warteri is still in the exploration stage at home and abroad, mainly focuses on the aspects of biological characteristics, breeding modes, nutrient analysis and the like, and is lack of sufficient understanding on the living habits, physiological ecology, fertilization development and the like related to reproductive biology. Although some scholars try to carry out artificial hatching and fry breeding of the rhynchophorus warteri, the method actually simply puts wild parent insects together to enable the wild parent insects to naturally lay eggs and fertilize the wild parent insects, and has the defects of long egg laying time, small egg laying amount, time and labor consumption and incapability of being applied to production in a large scale. Therefore, the artificial induction and incubation of the rhynchophorus warteri are very necessary to be carried out, and a foundation is laid for seed cultivation and breeding, germplasm resource protection and market demand meeting in the future.
Disclosure of Invention
The invention aims to solve the defects of the prior art and provides an artificial induced spawning and hatching method of the Orychophragmus wartius, so as to improve the spawning amount, fertility rate and hatching rate of the Orychophragmus wartius during breeding.
In order to achieve the purpose, the invention adopts the following technical scheme:
an artificial induced spawning and hatching method of the rhynchophorus warteri comprises the following specific steps:
(1) preparation before induction of labor: precipitating, filtering, disinfecting and aerating the extracted seawater about 7 days before the seawater climbs in 7-9 months of the lunar calendar every year; meanwhile, the stored pure fresh water is filtered, disinfected and aerated in the same way to kill harmful microorganisms in the water body; then, the water for induced spawning is prepared; adjusting the water temperature to make the seawater and fresh water have the same temperature;
(2) parent selection: selecting wild or cultured female and male Aristolochia grunescens as parent for propagation, and requiring the larvae to be plump and stout, bright and bright in body surface color, and to move rapidly and be alive without diseases and injuries;
(3) artificial insemination: putting the selected female and male parent of the rhynchophorus ferrugineus into an induced spawning basin/pool filled with water in advance, naturally swimming for 10-15 minutes in the induced spawning basin/pool, fishing out the larva by using a 100-mesh bolting silk net when the female and male parents usually do rotary motion or the male revolves around the female, extruding the ovum and sperm into the basin by lightly pressing the head to the tail of the larva with hands, continuously stirring the water body, fully combining the sperm and the ovum, completing fertilization, and removing the parent worm;
(4) and (3) washing eggs: letting the fertilized eggs stand still in an induced spawning basin/pool for 5-10 minutes, and collecting the fertilized eggs by using a 200-mesh fishing net after the fertilized eggs are completely sunk into the bottom of the basin/pool, and washing the fertilized eggs with water;
(5) artificial incubation: and (3) putting the cleaned fertilized eggs with water into an incubation barrel for aeration incubation, wherein the fertilized eggs are required to be suspended in a water body.
Further, the seawater used in the step (1) needs to be precipitated for 2-3 days, and then is subjected to sand filtration and ultraviolet disinfection; pure fresh water is subjected to carbon filtration and is used at a ratio of 10-15/m3The quicklime is sterilized, the dissolved oxygen in the water body is above 5mg/L, and the pH is above 7.5.
Further, the method for preparing the water for hastening parturition in the step (1) comprises the following steps: firstly, adding one third of sterilized seawater into an oxytocic pool/basin, then adding fresh water, simultaneously adding fresh water, and measuring the salinity of a prepared water body by using a salinity meter until the salinity is measured to be 15 per mill; then, the dissolved oxygen of the induced spawning water body is kept at 5mg/L through micropore aeration; wherein the temperature of the sea water and the fresh water is between 25 and 30 ℃.
Further, in the step (2), the weight of the parent female verruca kistroglorys is more than 1 g/tail, the abdomen and wart feet of the polypide are white, the setae is pulpous, the back of the polypide is smooth and has no mastoid, and the color of the body except the head is light green or turquoise; male verruca Humata Linnaeus has weight of 1.5 g/tail, white abdomen and wart foot, pulpiness, papilla on dorsal beard of wart foot, chrysanthemum papilla around anus, dark red tail, and a longitudinal milky white strip in the middle, except for head, dark pink.
Further, the ratio of the female and male parent of the female wart and the male parent of the kissing silkworm induced by artificial spawning in the step (3) is 3: 1; the salinity of the water in the induced spawning basin/pool is 15 per mill, the pH is 8.0-8.5, the temperature is 25-30 ℃, and the dissolved oxygen is more than 5 mg/L; when the extruded parents are fertilized, the actions are required to be soft and rapid and the extruded parents cannot leave the water body, and the whole artificial induced spawning process must be completed within 30 minutes.
Furthermore, the salinity, temperature and pH of the water body used for flushing the fertilized eggs in the step (4) are consistent with the original water body in the induced spawning basin/pool; the egg washing action needs to be gentle and quick; the fertilized egg must be washed 2-3 times to wash away excess sperm mixture and other organic matter in the water.
Further, the salinity, temperature and pH of the water used in the hatching barrel in the step (5) are consistent with those in the induced spawning basin/pool, and the fertilized eggs must be always in a suspension state, and the development condition of the fertilized eggs is checked every day; and from the day of incubation to the larva stage of the third conus, replacing part of the water body every day, and ensuring that the salinity of the incubation water body is reduced by 2-3 per mill every day.
Further, in the step (5), the salinity, temperature and pH of the water in the hatching barrel and the water in the fry pond must be kept consistent when the fry enters the fry pond.
The invention has the following remarkable advantages:
the invention fully utilizes the reproductive biological characteristics of the verrucosa, artificially simulates the water area environmental conditions during natural reproduction, and solves the problems of the verrucosa reproduction water environmental conditions, the selection of the seed insects, the male and female parent proportion, the fertilization time and the incubation technology through artificial assisted induced spawning and innovative optimization of the incubation method, thereby shortening the spawning time, and compared with natural reproduction, the spawning amount is improved by more than 80 percent, and the fertilization rate and the incubation rate reach more than 95 percent. Realizes the large-scale artificial breeding of the wart Husky silkworm, lays a foundation for protecting germ plasm resources of the wart Husky silkworm, developing proliferation and releasing and artificial breeding, and has important significance for the intensive development of the China Husky silkworm industry.
Detailed Description
The present invention is further illustrated by the following examples, which are not intended to limit the scope of the invention.
Example 1:
and 8, 8 and 14 days in 2019, carrying out artificial induced spawning breeding on the parents of the Nereis grunniens purchased from Minjiang to Haikou in Nereis breeding bases of Yangze ocean biotechnology limited company in Fujian province. The method specifically comprises the following steps:
(1) preparation before induction of labor: and 8, 6 days after 8 months, pumping the seawater into a sedimentation tank, and after 2 days of sedimentation, disinfecting the seawater by sand filtration and ultraviolet rays and then feeding the seawater into a seawater aeration tank for later use. Simultaneously pumping river water into a sedimentation tank for sedimentation for 3 days, and then carrying outFiltering with sand and activated carbon, and reusing at 15/m3The quicklime is sterilized, and the micro-pore aeration is carried out, the dissolved oxygen in the water body is measured to be 8mg/L, the pH value is 7.8, and the temperature of the sea water and the fresh water is consistent to be 28 +/-0.5 ℃.
(2) Parent selection: the parent of the Orychophragmus warts is a natural wild species, and has the advantages of full and stout body, bright and bright body surface color, rapid motility, no disease and no injury. The average weight of female wart snout clamworm is 1.2 + -0.05 g/tail, the body length is 5-7 cm/tail, the abdomen and wart feet of the female wart are whitish, the setae is pulpous, the back of the female wart is smooth and has no mastoid, and the body color except the head is light green or turquoise green; male verruca Humata Linnaeus has body weight of 1.6 + -0.01 g/tail, body length of 7-9 cm/tail, white abdomen and wart foot, pulpous bristles, papilla on dorsal beard of wart foot, chrysanthemum papilla around anus, dark red tail, and a longitudinal milky white strip in the middle, and has dark pink color except head.
(3) Artificial insemination: a plastic basin with the diameter of 83cm and the height of 18cm is used as an induced spawning basin, 30 female silkworms and 10 male silkworms of the selected rhynchophorus are placed in the basin to naturally swim for 10 minutes, a screen silk net with 100 meshes is used for picking up the worms, the worms are lightly squeezed from the heads to the tails of the worms by hands, eggs or sperms are quickly squeezed into the basin, meanwhile, water is continuously stirred, so that sperms and eggs are fully combined, fertilization is completed, and parent worms are removed.
(4) And (3) washing eggs: and (3) allowing the fertilized eggs to stand still in the induced spawning basin/pool for 10 minutes, using a 200-mesh fishing net to collect the fertilized eggs after the fertilized eggs are completely sunk into the bottom of the basin/pool, washing the fertilized eggs with water for 2 times, and removing excessive sperm mixing and other organic matters in the water body.
(5) Artificial incubation: and putting the cleaned fertilized eggs with water into an incubation barrel (the incubation barrel is an inverted cone), inflating for incubation, and suspending the fertilized eggs in a water body. The salinity, temperature and pH of the water used in the hatching bucket are consistent with those in the oxytocin basin/pool. From the second day of incubation to the third congol larva stage, part of the water body is replaced every day, and the salinity of the incubation water body is reduced by 2 per mill every day. When the fry enters the fry raising pond, the salinity, temperature, pH value, etc. of the water in the hatching barrel and the water in the fry pond must be kept consistent.
Example 2:
and in 2019, 8 and 31, artificially induced spawning and breeding of the Oncorhynchus verrucosus parent purchased from Nidofn, in Nereid breeding base of Yangze ocean biotechnology Limited company in Fujian province. The method specifically comprises the following steps:
(1) preparation before induction of labor: and after 8 months and 20 days, pumping the seawater into a sedimentation tank, and after 5 days of sedimentation, sterilizing the seawater by sand filtration and ultraviolet rays, and then feeding the seawater into a seawater aeration tank for later use. Simultaneously pumping river water into a sedimentation tank for sedimentation for 4 days, filtering with sand and active carbon, and then using 10/m3The quicklime is sterilized, and the micro-pore aeration is carried out, the dissolved oxygen in the water body is 10mg/L, the pH is 7.5, and the temperature of the sea water and the fresh water is consistent to 27.5 +/-0.5 ℃.
(2) Parent selection: the parent of the Orychophragmus warts is a natural wild species, and has the advantages of full and stout body, bright and bright body surface color, rapid motility, no disease and no injury. The average weight of female wart snout clamworm is 1.0 + -0.03 g/tail, the body length is 5-6 cm/tail, the abdomen and wart feet of the female wart are whitish, the setae is pulpous, the back of the female wart is smooth and has no mastoid, and the body color except the head is light green or turquoise green; male verruca Humata Linnaeus has body weight of 1.5 + -0.01 g/tail, body length of 7-8 cm/tail, white abdomen and wart foot, pulpous bristles, papilla on dorsal beard of wart foot, chrysanthemum papilla around anus, dark red tail, and a longitudinal milky white strip in the middle, and has dark pink color except head.
(3) Artificial insemination: a plastic basin with the diameter of 83cm and the height of 18cm is used as an induced spawning basin, 120 female silkworms and 40 male silkworms of the selected rhynchophorus are placed in the basin to naturally swim for 15 minutes, a screen silk net with 100 meshes is used for picking up the worms, the worms are lightly squeezed from the heads to the tails of the worms by hands, eggs or sperms are quickly squeezed into the basin, meanwhile, water is continuously stirred, so that sperms and eggs are fully combined, fertilization is completed, and parent worms are removed.
(4) And (3) washing eggs: and (3) allowing the fertilized eggs to stand still in the induced spawning basin/pool for 15 minutes, using a 200-mesh fishing net to collect the fertilized eggs after the fertilized eggs are completely sunk into the bottom of the basin/pool, washing the fertilized eggs with water for 3 times, and removing excessive sperm mixing and other organic matters in the water body.
(5) Artificial incubation: and putting the cleaned fertilized eggs with water into an incubation barrel (the incubation barrel is an inverted cone), inflating for incubation, and suspending the fertilized eggs in a water body. The salinity, temperature and pH of the water used in the hatching bucket are consistent with those in the oxytocin basin/pool. From the second day of incubation to the third congol larva stage, part of the water body is replaced every day, and the salinity of the incubation water body is reduced by 2 per mill every day. When the fry enters the fry raising pond, the salinity, temperature, pH value, etc. of the water in the hatching barrel and the water in the fry pond must be kept consistent.
Example 3:
and in 2019, 9 and 13, artificially hastening parturition and breeding of the parents of the Nereis verrucosus purchased from Wenzhou, Zhejiang in Nereis breeding base of Yangze ocean biotechnology limited company in Fujian province. The method specifically comprises the following steps:
(1) preparation before induction of labor: and 9, 3 days after 9 months, pumping the seawater into a sedimentation tank, and after 2 days of sedimentation, sterilizing the seawater by sand filtration and ultraviolet rays and then feeding the seawater into a seawater aeration tank for later use. Simultaneously pumping river water into a sedimentation tank for sedimentation for 3 days, filtering with sand and active carbon, and then using 10/m3The quicklime is sterilized, and the micro-pore aeration is carried out, the dissolved oxygen in the water body is measured to be 9mg/L, the pH value is 7.7, and the temperature of the sea water and the fresh water is consistent to be 28.5 +/-0.3 ℃.
(2) Parent selection: the parent of the Orychophragmus warts is a natural wild species, and has the advantages of full and stout body, bright and bright body surface color, rapid motility, no disease and no injury. The average weight of female wart snout clamworm is 1.5 + -0.2 g/tail, the body length is 6-8 cm/tail, the abdomen and wart feet of the worm body are whitish, the setae is pulpous, the back of the body is smooth without mastoid, and the body is light green or turquoise except the head; male verruca Humata Linnaeus has body weight of 1.8 + -0.3 g/tail, body length of 8-9 cm/tail, white abdomen and wart foot, pulpous bristles, papilla on dorsal beard of wart foot, chrysanthemum papilla around anus, dark red tail, and a longitudinal milky white strip in the middle, and has dark pink color except head.
(3) Artificial insemination: a plastic basin with the diameter of 83cm and the height of 18cm is used as an induced spawning basin, 60 female silkworms and 20 male silkworms of the selected rhynchophorus are placed in the basin to naturally swim for 10 minutes, a screen silk net with 100 meshes is used for picking up the worms, the worms are lightly squeezed from the heads to the tails of the worms by hands, eggs or sperms are quickly squeezed into the basin, meanwhile, water is continuously stirred, so that sperms and eggs are fully combined, fertilization is completed, and parent worms are removed.
(4) And (3) washing eggs: and (3) allowing the fertilized eggs to stand still in the induced spawning basin/pool for 10 minutes, using a 200-mesh fishing net to collect the fertilized eggs after the fertilized eggs are completely sunk into the bottom of the basin/pool, washing the fertilized eggs with water for 3 times, and removing excessive sperm mixing and other organic matters in the water body.
(5) Artificial incubation: and putting the cleaned fertilized eggs with water into an incubation barrel (the incubation barrel is an inverted cone), inflating for incubation, and suspending the fertilized eggs in a water body. The salinity, temperature and pH of the water used in the hatching bucket are consistent with those in the oxytocin basin/pool. From the second day of incubation to the third congol larva stage, part of the water body is replaced every day, and the salinity of the incubation water body is reduced by 2 per mill every day. When the fry enters the fry raising pond, the salinity, temperature, pH value, etc. of the water in the hatching barrel and the water in the fry pond must be kept consistent.
The above description is only a preferred embodiment of the present invention, and all equivalent changes and modifications made in accordance with the claims of the present invention should be covered by the present invention.
Claims (8)
1. An artificial induced spawning and hatching method of the rhynchophorus warteri comprises the following specific steps:
(1) preparation before induction of labor: precipitating, filtering, disinfecting and aerating the extracted seawater about 7 days before the seawater climbs in 7-9 months of the lunar calendar every year; meanwhile, the stored pure fresh water is filtered, disinfected and aerated in the same way to kill harmful microorganisms in the water body; then, the water for induced spawning is prepared; adjusting the water temperature to make the seawater and fresh water have the same temperature;
(2) parent selection: selecting wild or cultured female and male Aristolochia grunescens as parent for propagation, and requiring the larvae to be plump and stout, bright and bright in body surface color, and to move rapidly and be alive without diseases and injuries;
(3) artificial insemination: putting the selected female and male parent of the rhynchophorus ferrugineus into an induced spawning basin/pool filled with water in advance, naturally swimming for 10-15 minutes in the induced spawning basin/pool, fishing out the larva by using a 100-mesh bolting silk net when the female and male parents usually do rotary motion or the male revolves around the female, extruding the ovum and sperm into the basin by lightly pressing the head to the tail of the larva with hands, continuously stirring the water body, fully combining the sperm and the ovum, completing fertilization, and removing the parent worm;
(4) and (3) washing eggs: letting the fertilized eggs stand still in an induced spawning basin/pool for 5-10 minutes, and collecting the fertilized eggs by using a 200-mesh fishing net after the fertilized eggs are completely sunk into the bottom of the basin/pool, and washing the fertilized eggs with water;
(5) artificial incubation: and (3) putting the cleaned fertilized eggs with water into an incubation barrel for aeration incubation, wherein the fertilized eggs are required to be suspended in a water body.
2. The artificial oxytocic and hatching method of the Nereid warts according to claim 1, which is characterized in that: the seawater used in the step (1) needs to be precipitated for 2-3 days, and is subjected to sand filtration and ultraviolet disinfection in sequence; pure fresh water is subjected to carbon filtration and is used at a ratio of 10-15/m3The quicklime is sterilized, the dissolved oxygen in the water body is above 5mg/L, and the pH is above 7.5.
3. The artificial oxytocic and hatching method of the Nereid warts according to claim 1, which is characterized in that: the preparation method of the water for induction of labor in the step (1) comprises the following steps: firstly, adding one third of sterilized seawater into an oxytocic pool/basin, then adding fresh water, simultaneously adding fresh water, and measuring the salinity of a prepared water body by using a salinity meter until the salinity is measured to be 15 per mill; then, the dissolved oxygen of the induced spawning water body is kept at 5mg/L through micropore aeration; wherein the temperature of the sea water and the fresh water is between 25 and 30 ℃.
4. The artificial oxytocic and hatching method of the Nereid warts according to claim 1, which is characterized in that: in the step (2), the weight of the parent female verruca kistroglorys is more than 1 g/tail, the abdomen and wart feet of the polypide are whitish, the setae is pasty, the back of the polypide is smooth and has no mastoid, and the color of the body except the head is light green or turquoise; male verruca Humata Linnaeus has weight of 1.5 g/tail, white abdomen and wart foot, pulpiness, papilla on dorsal beard of wart foot, chrysanthemum papilla around anus, dark red tail, and a longitudinal milky white strip in the middle, except for head, dark pink.
5. The artificial oxytocic and hatching method of the Nereid warts according to claim 1, which is characterized in that: the ratio of the female and male parent of the male wart kiss silkworm induced by artificial induced spawning in the step (3) is 3: 1; the salinity of the water in the induced spawning basin/pool is 15 per mill, the pH is 8.0-8.5, the temperature is 25-30 ℃, and the dissolved oxygen is more than 5 mg/L; when the extruded parents are fertilized, the actions are required to be soft and rapid and the extruded parents cannot leave the water body, and the whole artificial induced spawning process must be completed within 30 minutes.
6. The artificial oxytocic and hatching method of the Nereid warts according to claim 1, which is characterized in that: the salinity, temperature and pH of the water body used for flushing the fertilized eggs in the step (4) are consistent with the original water body in the induced spawning basin/pool; the egg washing action needs to be gentle and quick; the fertilized egg must be washed 2-3 times to wash away excess sperm mixture and other organic matter in the water.
7. The artificial oxytocic and hatching method of the Nereid warts according to claim 1, which is characterized in that: the salinity, temperature and pH of the water used by the hatching barrel in the step (5) are consistent with those in the induced spawning basin/pool, and the fertilized eggs are required to be always in a suspension state, and the development condition of the fertilized eggs is checked every day; and from the day of incubation to the larva stage of the third conus, replacing part of the water body every day, and ensuring that the salinity of the incubation water body is reduced by 2-3 per mill every day.
8. The artificial oxytocic and hatching method of the Nereid warts according to claim 1, which is characterized in that: in the step (5), when the fry enters the fry breeding pond, the salinity, the temperature and the pH of the water in the hatching barrel and the water in the fry pond are required to be consistent.
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| CN112450132A (en) * | 2020-11-24 | 2021-03-09 | 阳江职业技术学院 | Method for artificially hastening parturition and hatching of rhynchophorus wartius |
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