CN102499159B - Phoxinus lagowskii fish fry breeding method - Google Patents
Phoxinus lagowskii fish fry breeding method Download PDFInfo
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- CN102499159B CN102499159B CN2011103616115A CN201110361611A CN102499159B CN 102499159 B CN102499159 B CN 102499159B CN 2011103616115 A CN2011103616115 A CN 2011103616115A CN 201110361611 A CN201110361611 A CN 201110361611A CN 102499159 B CN102499159 B CN 102499159B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
A phoxinus lagowskii fish fry breeding method relates to a fish fry breeding method and aims to resolve problems that existing phoxinus lagowskii fish fries are fished in natural water areas, the quantity of the fish fries is low, the quantity of half-bred fish fries is high, and the existing phoxinus lagowskii fish fries cannot be widely popularized and cultivated. The phoxinus lagowskii fish fry breeding method comprises steps: firstly, selecting male and female parent fishes according to needs; secondly, preparing spawn inducing medicine; thirdly, injecting the spawn inducing medicine to induce spawning of the parent fishes; fourthly, realizing artificial insemination: carrying out artificial egg squeezing and sperm squeezing, and carrying out dry-method insemination to obtain fertilized eggs; and fifthly, placing the fertilized eggs in an incubator to be incubated. Induced spawning rate of the female parent fishes which are cultivated artificially can reach 60% to 70%, and is increased by 50% to 60% as compared with the spawning rate of parent fishes without being induced, inducing success rate of the male parent fishes reaches 100%, incubated fish fry yield can reach 80% to 93%, and further, incubated fish fries are pure phoxinus lagowskii fish fries. The phoxinus lagowskii fish fry breeding method has the advantages of environmental protection, simplicity, convenience, low cost, easiness in popularization and the like, and is used for breeding phoxinus lagowskii fish fries.
Description
Technical field
The present invention relates to the propagation method of fry.
Background technology
Luo Shi Minnow (Phoxinus lagowskii Dybowski), the former name long-tail
, Laplace
Northeast local name Liu Genchi.Cyprinidae, Leuciscinae,
Belong to.Sexual maturity in 2 years is laid eggs in batches, and omnivorousness likes living in cold water domain, is small fishes.
Belong to fish and be distributed with 7 kinds in China, all belong to small fishes, general individual is all below 10 centimeters, and body weight is lived in the tributary in each rivers, lake below 10 grams, likes cold water domain.The meat amount is high because good, the fish body of its Migration environment contains, thorn less between flesh, meat flavour is delicious, is loved by the people.Reproduction period is in 5～June, and particularly northeastward, the northwest, grow simultaneously in same waters and the kind of breeding has 3～4 kinds.Luo Shi Minnow is wherein individual larger a kind of, propagates individual total length artificially and can reach more than 20 centimeters, and body weight can obtain more than 50 grams.
Summary of the invention
The present invention will solve existing Luo Shi Minnow fry to fish at natural water area, exists the problem that few, the assorted seedling of fry quantity is many, can't spread cultivate, and a kind of Luo Shi Minnow fish breeding method is provided.
The concrete steps of a kind of Luo Shi Minnow fish breeding method of the present invention are: one, select parent population: late May, choose do not have the reproduction projection, body length more than the 14cm, body weight is the female parent population more than the 31g, more than 3 ages, with have reproduction projection, body length more than the 13cm, body weight is the male parent population more than the 32g, more than 3 ages, female parent population is 1: 1～2 with the quantity ratio of male parent population, selected parent population is put into storage pond, holding pond, and the storage pond, holding pond water temperature is 12～18 ℃; Two, preparation oxytocic drug: before hastening parturition, prepare oxytocic drug, the oxytocic drug of female parent population needs the ratio preparation of 2mg maleic acid DOM and 20ug salmon releasing hormone analog in the female parent population of per kilogram, the oxytocic drug of male parent population needs the ratio preparation of 1mg maleic acid DOM and 10ug salmon releasing hormone analog in the male parent population of per kilogram; Three, hasten parturition: the oxytocic drug of step 2 preparation is expelled to thoracic cavity or the dorsal fin base portion of parent population, the parent population behind the injectable drug is put back in the storage pond, holding pond; Four, artificial insemination: the injection oxytocic drug manually squeezed ovum, squeezes essence after 72～75 hours, carried out the dry method insemination, obtained fertilized egg; Five, hatching: fertilized egg is sticked to through on the polyethylene screen cloth or fortune windmillpalm sheath-fibre of sterilizing, put into the incubator box through sterilization, Spawning water is cycle water in the incubator box, water temperature is 12～18 ℃, oxygen in water remains on more than the 5mg/L, every other day fish-egg is taken out with 2ppm methylene blue sterilization 5 minutes, puts back in the incubator box again, whole incubation period accumulated temperature needs 78～100 to subsist, and obtains Luo Shi Minnow fry after 5～7 days.
The invention has the beneficial effects as follows: male and female difference, the specification of parent population that the present invention is clear and definite, stipulated oxytocic drug kind, dose and water temperature range, illustrated the hatch fish roe method.The spawning rate that the present invention hastens parturition to the female parent population of propagating artificially can reach 60%～70%, the Gunther parent fish spawning rate of more not hastening parturition improves 50%～60%, disposable number of eggs laid can reach 40%～50% of total fish brood amount, 100% to the male parent population success rate of hastening parturition, the hatching emergence rate can reach 80%～93%, and fry is purebred Luo Shi Minnow fry, can the spread cultivation.The inventive method have environmental protection, easy and simple to handle, with low cost, be easy to the advantages such as popularization, for the large-scale cultivation marketable fish is taken a firm foundation.
A kind of Luo Shi Minnow fish breeding method of the present invention is used for the artificial propagation of Luo Shi Minnow fry.
Embodiment
Technical solution of the present invention is not limited to following cited embodiment, also comprises any combination between each embodiment.
Embodiment one: the propagation method of Luo Shi Minnow fry is carried out according to the following steps: one, select parent population: late May, choose do not have the reproduction projection, body length more than the 14cm, body weight is the female parent population more than the 31g, more than 3 ages, with have reproduction projection, body length more than the 13cm, body weight is the male parent population more than the 32g, more than 3 ages, female parent population is 1: 1～2 with the quantity ratio of male parent population, selected parent population is put into storage pond, holding pond, and the storage pond, holding pond water temperature is 12～18 ℃; Two, preparation oxytocic drug: before hastening parturition, prepare oxytocic drug, the oxytocic drug of female parent population needs the ratio preparation of 2mg maleic acid DOM and 20ug salmon releasing hormone analog in the female parent population of per kilogram, the oxytocic drug of male parent population needs the ratio preparation of 1mg maleic acid DOM and 10ug salmon releasing hormone analog in the male parent population of per kilogram; Three, hasten parturition: the oxytocic drug of step 2 preparation is expelled to thoracic cavity or the dorsal fin base portion of parent population, the parent population behind the injectable drug is put back in the storage pond, holding pond; Four, artificial insemination: the injection oxytocic drug manually squeezed ovum, squeezes essence after 72～75 hours, carried out the dry method insemination, obtained fertilized egg; Five, hatching: fertilized egg is sticked to through on the polyethylene screen cloth or fortune windmillpalm sheath-fibre of sterilizing, put into the incubator box through sterilization, Spawning water is cycle water in the incubator box, water temperature is 12～18 ℃, oxygen in water remains on more than the 5mg/L, every other day fish-egg is taken out with 2ppm methylene blue sterilization 5 minutes, puts back in the incubator box again, whole incubation period accumulated temperature needs 78～100 to subsist, and obtains Luo Shi Minnow fry after 5～7 days.
The feature of difference parent population male and female is in the present embodiment step 1: male parent population gonopore has long gonapophysis, and raun does not have.In the present embodiment parent population select and the process action of hastening parturition wants soft, needle for injection will be with 75% alcohol-pickled, other used tool will be sterilized through povidone iodine, disposable oxytocic drug is expelled in the parent population body.Step 3, four described screen clothes or fortune windmillpalm sheath-fibre, incubator box carry out disinfection with povidone iodine.Approximately after the Ninth Heaven, the fry floating is taken out screen cloth.
The beneficial effect of present embodiment is: male and female difference, the specification of parent population that present embodiment is clear and definite, stipulated oxytocic drug kind, dose and water temperature range, and illustrated the hatch fish roe method.The spawning rate that the present invention hastens parturition to the female parent population of propagating artificially can reach 60%～70%, the Gunther parent fish spawning rate of more not hastening parturition improves 50%～60%, disposable number of eggs laid can reach 40%～50% of total fish brood amount, 100% to the male parent population success rate of hastening parturition, the hatching emergence rate can reach 80%～93%, and fry is purebred Luo Shi Minnow fry, can the spread cultivation.The inventive method have environmental protection, easy and simple to handle, with low cost, be easy to the advantages such as popularization, for the large-scale cultivation marketable fish is taken a firm foundation.
Embodiment two: what present embodiment and embodiment one were different is that parent population is respectively that body length is that 14～17cm, body weight are the above female parent population in three ages of 31～58g in the step 1, and body length is that 13～15cm, body weight are the above male parent population in three ages of 32～50g.Other is identical with embodiment one.
Embodiment three: present embodiment is different from embodiment one or two is that the quantity ratio of male and female parent population in the step 1 is 1: 1.5.Other is identical with embodiment one or two.
Embodiment four: what present embodiment was different from one of embodiment one to three is that oxytocic drug is that 4h prepares with interior before hastening parturition in the step 2, and under 0～4 ℃ of condition, is placed on the lucifuge place and preserves.Other is identical with one of embodiment one to three.
Embodiment five: present embodiment is different from one of embodiment one to four is that water temperature is 13～15 ℃ in the storage pond, holding pond in rapid three.Other is identical with one of embodiment one to four.
Embodiment six: present embodiment is different from one of embodiment one to four is that water temperature is 14 ℃ in the storage pond, holding pond in the step 3.Other is identical with one of embodiment one to four.
Embodiment seven: what present embodiment was different from one of embodiment one to six is that the injection oxytocic drug carried out artificial insemination after 73 hours in the step 4.Other is identical with one of embodiment one to six.
Embodiment eight: present embodiment is different from one of embodiment one to seven is that water temperature is 14 ℃ in the incubator box in the step 5.Other is identical with one of embodiment one to seven.
Embodiment nine: what present embodiment was different from one of embodiment one to eight is that the cycle water in the incubator box adopts inflator pump or oxygen cylinder oxygenation in the step 5.Other is identical with one of embodiment one to eight.
Embodiment ten: what present embodiment was different from one of embodiment one to nine is that oxygen in water remains on 5～10mg/L in the step 5.Other is identical with one of embodiment one to nine.
Embodiment 11: the propagation method of Luo Shi Minnow fry is carried out according to the following steps: one, select parent population: late May, choose do not have the reproduction projection, body length more than the 14cm, body weight is the female parent population more than the 31g, more than 3 ages, with have reproduction projection, body length more than the 13cm, body weight is the male parent population more than the 32g, more than 3 ages, female parent population is 2: 1 with the quantity ratio of male parent population, selected parent population is put into storage pond, holding pond, and the storage pond, holding pond water temperature is 14 ℃; Two, preparation oxytocic drug: the oxytocic drug of female parent population needs the ratio preparation of 2mg maleic acid DOM and 20ug salmon releasing hormone analog in the female parent population of per kilogram, the oxytocic drug of male parent population needs the ratio preparation of 1mg maleic acid DOM and 10ug salmon releasing hormone analog in the male parent population of per kilogram, hasten parturition front 2h with the interior oxytocic drug for preparing, and under 2 ℃ of conditions, be placed on the lucifuge place and preserve; Three, hasten parturition: the oxytocic drug of step 2 preparation is expelled to the thoracic cavity of parent population, the parent population behind the injectable drug is put back in the storage pond, holding pond; Four, artificial insemination: the injection oxytocic drug manually squeezed ovum, squeezes essence after 73 hours, carried out the dry method insemination, obtained fertilized egg; Five, the hatching: with fertilized egg stick to through the sterilization the polyethylene screen cloth, put into the incubator box through sterilization, Spawning water is cycle water in the incubator box, water temperature is 14 ℃, oxygen in water remains on 8mg/L, every other day fish-egg is taken out with 2ppm methylene blue sterilization 5 minutes, puts back in the incubator box again, whole incubation period accumulated temperature needs 90 to subsist, and obtains Luo Shi Minnow fry after 6 days.
The beneficial effect of present embodiment is: male and female difference, the specification of parent population that present embodiment is clear and definite, stipulated oxytocic drug kind, dose and water temperature range, and illustrated the hatch fish roe method.The spawning rate that the present invention hastens parturition to the female parent population of propagating artificially can reach 70%, the Gunther parent fish spawning rate of more not hastening parturition improves 60%, disposable number of eggs laid can reach 43.58% of total fish brood amount, 100% to the male parent population success rate of hastening parturition, the hatching emergence rate can reach 92.72%, and fry is purebred Luo Shi Minnow fish, can the spread cultivation.The inventive method have environmental protection, easy and simple to handle, with low cost, be easy to the advantages such as popularization, for the large-scale cultivation marketable fish is taken a firm foundation.
Claims (15)
1. the propagation method of a Luo Shi Minnow fry, the propagation method that it is characterized in that Luo Shi Minnow fry is carried out according to the following steps: one, select parent population: late May, choose do not have the reproduction projection, body length more than the 14cm, body weight is the female parent population more than the 31g, more than 3 ages, with have reproduction projection, body length more than the 13cm, body weight is the male parent population more than the 32g, more than 3 ages, female parent population is 1:1～2 with the quantity ratio of male parent population, selected parent population is put into storage pond, holding pond, and the storage pond, holding pond water temperature is 12～18 ℃; Two, preparation oxytocic drug: before hastening parturition, prepare oxytocic drug, the oxytocic drug of female parent population needs the ratio preparation of 2mg maleic acid DOM and 20ug salmon releasing hormone analog in the female parent population of per kilogram, the oxytocic drug of male parent population needs the ratio preparation of 1mg maleic acid DOM and 10ug salmon releasing hormone analog in the male parent population of per kilogram; Three, hasten parturition: the oxytocic drug of step 2 preparation is expelled to thoracic cavity or the dorsal fin base portion of parent population, the parent population behind the injectable drug is put back in the storage pond, holding pond; Four, artificial insemination: the injection oxytocic drug manually squeezed ovum, squeezes essence after 72～75 hours, carried out the dry method insemination, obtained fertilized egg; Five, hatching: fertilized egg is sticked to through on the polyethylene screen cloth or fortune windmillpalm sheath-fibre of sterilizing, put into the incubator box through sterilization, Spawning water is cycle water in the incubator box, water temperature is 12～18 ℃, oxygen in water remains on more than the 5mg/L, every other day fish-egg is taken out with 2ppm methylene blue sterilization 5 minutes, puts back in the incubator box again, whole incubation period accumulated temperature needs 78～100 to subsist, and obtains Luo Shi Minnow fry after 5～7 days.
2. the propagation method of a kind of Luo Shi Minnow fry according to claim 1, it is characterized in that parent population is respectively that body length is that 14～17cm, body weight are the above female parent population in 3 ages of 31～58g in the step 1, body length is that 13～15cm, body weight are the above male parent population in 3 ages of 32～50g.
3. the propagation method of a kind of Luo Shi Minnow fry according to claim 1 is characterized in that the quantity of male and female parent population in the step 1 is than being 1:1.5.
4. according to claim 1, the propagation methods of 2 or 3 described a kind of Luo Shi Minnow fries, it is characterized in that oxytocic drug is that 4h prepares with interior before hastening parturition in the step 2, and under 0～4 ℃ of condition, be placed on the lucifuge place and preserve.
5. the propagation method of a kind of Luo Shi Minnow fry according to claim 4 is characterized in that water temperature is 13～15 ℃ in the storage pond, holding pond in the step 3.
6. the propagation method of a kind of Luo Shi Minnow fry according to claim 4 is characterized in that water temperature is 14 ℃ in the storage pond, holding pond in the step 3.
7. according to claim 1, the propagation methods of 2 or 3 described a kind of Luo Shi Minnow fries, it is characterized in that the injection oxytocic drug carried out artificial insemination after 73 hours in the step 4.
8. the propagation method of a kind of Luo Shi Minnow fry according to claim 6 is characterized in that water temperature is 14 ℃ in the incubator box in the step 5.
9. the propagation method of a kind of Luo Shi Minnow fry according to claim 1 and 2 is characterized in that the cycle water in the incubator box adopts inflator pump or oxygen cylinder oxygenation in the step 5.
10. the propagation method of a kind of Luo Shi Minnow fry according to claim 1 and 2 is characterized in that oxygen in water remains on 5～10mg/L in the step 5.
11. the propagation method of a kind of Luo Shi Minnow fry according to claim 5 is characterized in that the injection oxytocic drug carried out artificial insemination after 73 hours in the step 4.
12. the propagation method of a kind of Luo Shi Minnow fry according to claim 5 is characterized in that the cycle water in the incubator box adopts inflator pump or oxygen cylinder oxygenation in the step 5.
13. the propagation method of a kind of Luo Shi Minnow fry according to claim 8 is characterized in that the cycle water in the incubator box adopts inflator pump or oxygen cylinder oxygenation in the step 5.
14. the propagation method of a kind of Luo Shi Minnow fry according to claim 5 is characterized in that oxygen in water remains on 5～10mg/L in the step 5.
15. the propagation method of a kind of Luo Shi Minnow fry according to claim 8 is characterized in that oxygen in water remains on 5～10mg/L in the step 5.
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Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102715115A (en) * | 2012-07-09 | 2012-10-10 | 中国水产科学研究院黑龙江水产研究所 | Phoxinus lagowskii fry breeding method |
| CN102835336A (en) * | 2012-09-17 | 2012-12-26 | 盐城市盐都区水产技术推广站 | Method for batch breeding of loach fry |
| CN103960188B (en) * | 2014-05-20 | 2016-03-30 | 中国水产科学研究院长江水产研究所 | A kind of long Qi Wen Minnow induced spawning method |
| CN104488787B (en) * | 2014-12-18 | 2016-11-16 | 中国水产科学研究院黑龙江水产研究所 | A kind of propagation method of Coregonus ussuriensis fry |
| CN105850807B (en) * | 2016-04-25 | 2018-05-29 | 吉林省水产科学研究院 | A kind of artificial fecundation method of La Shi Minnow fishes |
| CN107182866A (en) * | 2017-07-17 | 2017-09-22 | 东北林业大学 | A kind of Hu Minnow artificial breeding method |
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| CN1460413A (en) * | 2003-01-24 | 2003-12-10 | 曾照军 | Method and device for breeding salmon |
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