CN102626057A - Method for artificially breeding young paphia textzle gmelin - Google Patents
Method for artificially breeding young paphia textzle gmelin Download PDFInfo
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- CN102626057A CN102626057A CN2012101090129A CN201210109012A CN102626057A CN 102626057 A CN102626057 A CN 102626057A CN 2012101090129 A CN2012101090129 A CN 2012101090129A CN 201210109012 A CN201210109012 A CN 201210109012A CN 102626057 A CN102626057 A CN 102626057A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract
The invention discloses a method for artificially breeding young paphia textzle gmelin. After searching, the researches on paphia textzle gmelin at home and abroad are just rising, relevant reports are few and the researches relating to the breeding method of young paphia textzle gmelin are never disclosed in any technology. The large-scale breeding of young paphia textzle gmelin is carried out by the technical measures of strengthening parents, artificially hastening parturition, artificially incubating, choosing excellent D-typed larvae, culturing larva, metamorphosis-attaching larvae and culturing juvenile mollusks. The method for artificially breeding young paphia textzle gmelin, disclosed by the invention, has the characteristics of uniqueness and advancement, a higher academic level and a practical value; the supply and demand contradiction of paphia textzle gmelin can be fundamentally solved and the breeding industry chains of paphia textzle gmelin are formed along the coast of Beibu Gulf. The method for artificially breeding young paphia textzle gmelin is important for gradually recovering natural resources of paphia textzle gmelin and promoting sustainable development of artificial breeding of paphia textzle gmelin.
Description
Technical field
The present invention relates to the shellfish offspring breeding method, specifically be the artificial raise seedling method of picture-weaving in silk Ba Fei clam, belong to marine product and culture the seedling growing process field.
Background technology
Picture-weaving in silk Ba Fei clam (paphis textile), Veneridae, the Ba Fei clam belongs to.Being commonly called as the cycle of sixty years king, is the coastal important economic shellfish of south China.Picture-weaving in silk Ba Fei clam is a kind of medium-sized marine shellfish, is long oval, and shell is hard, and the shell mass color is sliding, has the texture of rule, the solar month of 30 days face width, and shell inner face central authorities are pure white, can be made into beautiful handicraft.Picture-weaving in silk Ba Fei clam mainly is distributed in especially marine site, the North Sea one band of the South Sea in China, and the sandy end is liked in battalion's buried life in the end, generally under the suitable situation of substrate condition, transport phenomena on a large scale seldom occurs.Be fit to very much propagate artificially; Contain rich in protein and calcium, iron, zinc, iodine, selenium and other trace elements in the picture-weaving in silk Ba Fei clam meat; Its body contains a kind of material that is called tarine not only can reduce cholesterolemia concentration, and can make blood pressure drops, often eats this shellfish health is of great benefit to; Picture-weaving in silk Ba Fei clam is fresh and sweet with its meat, and is nutritious and liked by consumers in general.In addition, this shellfish also has advantages such as anti-accumulating, easy processing, so product enjoys a good market both at home and abroad.The North Sea is one of major production areas of China picture-weaving in silk Ba Fei clam, and along with the continuous of this shellfish price risen, local fisherman carries out destructive adopting to picture-weaving in silk Ba Fei clam and catches, and causes the rapid minimizing of picture-weaving in silk Ba Fei clam stock number in the North Sea in recent years.Through propagating artificially is to improve one of necessary channel that picture-weaving in silk Ba Fei clam population breeds, and artificial breeding provides the necessary condition of propagating artificially.
Through retrieval, the research of relevant picture-weaving in silk Ba Fei clam is just just risen both at home and abroad at present, and relevant report is few, and the disclosure that correlation technique is arranged is not more seen in the research that relates to picture-weaving in silk Ba Fei clam offspring breeding method.Therefore carry out seedling-cultivating method research, can alleviate the present situation that present picture-weaving in silk Ba Fei clam seed can only rely on the natural waters breeding, solve the problem that this kind is cultured the seed serious lack picture-weaving in silk Ba Fei clam.
Summary of the invention
The artificial raise seedling method that the purpose of this invention is to provide picture-weaving in silk Ba Fei clam; For the picture-weaving in silk Ba Fei clam resource of reasonable development with protection marine site, the North Sea; Carry out resource recovery regeneration; A kind of seedling growing process of realizing propagation in scale picture-weaving in silk Ba Fei clam artificial seed is provided, realizes the artificial increasing cultivation of picture-weaving in silk Ba Fei clam.
The realization of the object of the invention is that the artificial raise seedling method of picture-weaving in silk Ba Fei clam may further comprise the steps because taked following technical scheme:
(1) parent is strengthened: adopt and catch the natural waters ripe close shellfish in 2～3 ages; The back of returning is carried out body surface with the potassium permanganate sea water solution of 10ppm and is sterilized and be placed on preprepared close shellfish culture pond, and breeding density is placed 1～3 kilogram of close shellfish by every square of floor space, 50～100 centimetres of the close shellfish culture pond depth of waters; Ensure that the condition of water quality of close shellfish culture pond is 18～30 ℃ of water temperatures between parent shellfish culture period; Subsequent use close shellfish culture pond is prepared in seawater salinity 25 ‰～33 ‰, moves the pond every day once; Guarantee the nutrition supply of close shellfish; The high-quality of throwing something and feeding every day unit cell algae bait, feeding volume are per 5 kilograms of close shellfishes unit cell algae bait 30～10,000,000,000 cells of throwing something and feeding every day, and sampling is regularly dissected and carried out gonad development follow-up;
(2) artificial induced spawning: after gonad development gets into draining period, move into the temperature control case of hastening parturition through the close shellfish of above-mentioned cultivation and carry out artificial induced spawning through heating and cooling control; At first put into close shellfish the case of hastening parturition of 18～25 ℃ of water temperatures; 1～5 hour duration; Move into close shellfish the case of hastening parturition of 28～33 ℃ of water temperatures again, 0.5～2 hour duration, under the situation of close shellfish gonad maturity 1～3 time so repeatedly; The parent shellfish begins to arrange essence successively and lays eggs; The smart initial stage of laying eggs of close shellfish row through observing the smoke-like or the graininess of effluent, distinguish the male and female of close shellfish, by the close shellfish 10-14 of male and female: 1 ratio is evenly placed close shellfish and is gone into spawning pond; The overall control of each spawning pond parent shellfish is at 5～10 kilograms; The spawning pond water capacity is 10～25 cubic metres, and the condition of water quality of spawning pond is 25～30 ℃ of water temperatures, salinity 25 ‰～33 ‰, pH 7.8～8.3, DO＞6.5, COD＜1.5mg/L, content of nitrite＜0.15 μ g/L, hydrogen sulfide content＜0.05mg/L, and the close shellfish in the spawning pond was arranged essence successively and lays eggs and accomplish fertilization in 1～2 hour;
(3) artificial incubation: the fertilized egg of picture-weaving in silk Ba Fei clam can directly be hatched at spawning pond; 25 ℃～29 ℃ of control hatching water temperatures by the density of 3-10 fertilized egg of every milliliter of seawater cloth, keep the hatching water body to be in the fine motion state that emits minute bubbles through the Roots blower inflation; 25-50 minute fertilized egg begins division behind the insemination; Got into multicellular stage in 3-6 hour, 7-11 hour begins to rotate, and 12-18 hour develops into straight hinge larvas is " D " type larva;
(4) D type larva selected excellent: through 10～24 hours hatching process; The larva that those are second-rate and grow the fertilized egg go down and all can sink to the middle and lower part, pond; Adopt this moment and fish for or the method for siphon; Select for use 350 purpose bolting silk nets to drag for or mesh bag collects the high-quality young on top, put into ready nursery pond and cultivate;
(5) larvae cultivation: in nursery pond, cultivate by cultivating density the early stage of per 10 cubic metres of seawater cloth picture-weaving in silk Ba Fei clam larvas 300～8,000,000; Cultivating omnidistance the use is unit cell algae bait; Sooner or later the unit cell algae bait of throwing something and feeding every day twice, feeding volume are per 1,000,000 spats unit cell algae bait 50～10,000,000,000 cells of throwing something and feeding, and cultivate after 2～5 days to carry out 2 times every day and change water; Make the ocean temperature of cultivating in the pond keep 22～30 ℃; Salinity 25 ‰～28 ‰, the continuous conventional blowing aeration of cultivating process reaches more than 7 cultivation pond dissolved oxygen in water amount;
(6) larval metamorphosis adheres to: under the abundant condition of water temperature 25-28 ℃, bait; Picture-weaving in silk Ba Fei clam larva was through 8 days artificial culture, and shell length can reach 280～300 microns, and at this moment the larva face has begun atrophy; It is thus clear that bar-shaped foot is intermarginal flexible at two shells; Receive seedling with 150 purpose bolting silk mesh bags this moment, and the nursery pond of putting into collected larva the adherance that is prepared in advance impels it to adhere to, and it is a kind of plastic tab string that picture-weaving in silk Ba Fei clam larval metamorphosis adheres to employed adherance; Every string has 10-20 sheet rule or irregular plastic tab, and adherance 30～50 strings are thrown in every pond;
(7) juvenile mollusk is cultivated: the spat after metamorphosis is adhered to continues cultivation and has promptly got into the juvenile mollusk breeding phase, and the juvenile mollusk nurturing period keeps the water quality of nursery pond good, and nursery pond was wanted flowing water 2～5 hours every day; Every day the twice unit cell algae bait of throwing something and feeding; Feeding volume is per 1,000,000 spats unit cell algae bait 100～15,000,000,000 cells of throwing something and feeding, and the oxygen content of juvenile mollusk nurturing period larval rearing water is 6.8～8.1, cultivates through 7～10 days juvenile mollusks; Seed shell length can reach more than the 1mm; This moment, naked eyes on its shell can be observed picture-weaving in silk line shape, and through 10～15 days cultivation, the juvenile mollusk shell reached about 3mm again; Major part begins to fall down from attachment flaps and falls into nursery pond bottom, and this moment, spat was bred.
Said unit cell algae bait refer to marine green alga, chlorella, Isochrysis galbana, Dicrateria inornata or the Chaetoceros muelleri of gathering through artificial enlarged culture after introducing a fine variety, protect kind a kind of or combination in any contain algae liquid.
Take the present invention of above-mentioned measure, technological process is scientific and reasonable, and is workable.Can accomplish the scale breeding of picture-weaving in silk Ba Fei clam by the present invention's operation.At present, the market supply demand of picture-weaving in silk Ba Fei clam seed is very big, and the present invention can be described as and is a timely one, and has solved raiser's most pressing problem, the seedling and the technology that provide them to need most.The present invention has originality and advance, and higher academic level and practical value are arranged, and can fundamentally solve the imbalance between supply and demand of picture-weaving in silk Ba Fei clam, the littoral picture-weaving in silk Ba Fei clam aquaculture industry chain that forms in the North Sea.The present invention is to progressively recovering picture-weaving in silk Ba Fei clam natural resources amount, and the sustainable development that promotes picture-weaving in silk Ba Fei clam to propagate artificially is all significant.
Embodiment
Below through embodiment technical scheme of the present invention is further described.
Embodiment 1
The step of growing seedlings:
1. parent is strengthened: natural waters is adopted 36 kilograms of healthy close shellfishes of picture-weaving in silk Ba Fei clam that catch body surface smooth, 3 ages that shell is complete from marine site, the North Sea, Guangxi, carries out putting into preprepared close shellfish culture pond after body surface is sterilized with the potassium permanganate sea water solution of 10ppm.18 kilograms of close shellfishes are put in every pond in a suitable place to breed.Subsequent use close shellfish culture pond is 2 during the hot housing of parent shellfish, moves the pond every day once.Guarantee the nutrition supply of close shellfish, the high-quality of throwing something and feeding every day unit cell algae bait, feeding volume are every pond unit cell algae bait 20,000,000,000 cells of throwing something and feeding every day, 10:00 sampling carrying out gonad development follow-up in morning every day.
2, artificial induced spawning: above-mentioned close shellfish through hot housing is moved into the temperature control case of hastening parturition carry out artificial induced spawning after gonad development gets into draining period; Hasten parturition through heating and cooling control, at first put into close shellfish the case of hastening parturition of 20 ℃ of water temperatures, 3 hours duration; Move into close shellfish the case of hastening parturition of 28 ℃ of water temperatures again; 45 minutes duration repeated 2 times, and close shellfish begins to arrange essence successively and lays eggs.Distinguish male and female this moment; Put into spawning pond respectively 1,2, No. 3, put into 8 kilograms of the female shellfishes of picture-weaving in silk Ba Fei clam, 1 kilogram of male shellfish at No. 1 spawning pond; Put into 8 kilograms of the female shellfishes of picture-weaving in silk Ba Fei clam at No. 2 spawning ponds; 1 kilogram of male shellfish is put into 6 kilograms of the female shellfishes of picture-weaving in silk Ba Fei clam, 0.75 kilogram of male shellfish at No. 3 spawning pond spawning ponds.Unnecessary male shellfish is put back to close shellfish again and cultivates the pond.
3. artificial incubation: the fertilized egg through artificial induced spawning obtains is directly hatched at spawning pond; 26 ℃ of hatchery water temperatures; The every milliliter of seawater in No. 1 hatchery contains 6 fertilized eggs, and the every milliliter of seawater in No. 2 hatcheries contains 6 fertilized eggs, and the every milliliter of seawater in No. 3 hatcheries contains 4 fertilized eggs.Keep the hatching water body to be in the fine motion state that emits minute bubbles through the Roots blower inflation.35 minutes fertilized eggs begin division behind the insemination, get into multicellular stage in 4 hours 25 minutes, and 10 hours 10 minutes begin to rotate, and 15 hours develop into straight hinge larvas is " D " type larva;
4.D it is excellent that the type larva selects: through hatching, the larva that some are second-rate and grow the fertilized egg go down and all sink to the middle and lower part, pond is adopted the method for siphon, selects for use 350 purpose bolting silk mesh bags to collect the high-quality larva on top.
5. larvae cultivation: larva has been put into 6 nursery ponds through counting and has cultivated.Per 10 cubic metres of seawater cloth larvas 6,000,000 are divided in earlier stage at larval metamorphosis and to dredge, and at this moment per 10 cubic metres of seawater are put into larva quantity 3,500,000.Picture-weaving in silk Ba Fei clam larvae cultivation is omnidistance, and what use is unit cell algae bait, and the unit cell algae bait of throwing something and feeding sooner or later every day twice, feeding volume are per 1,000,000 spats unit cell algae bait 6,000,000,000 cells of throwing something and feeding.Observe larval growth every day and grow and the situation of ingesting, formulate the plan of throwing something and feeding on the same day.This picture-weaving in silk Ba Fei clam larvae cultivation changes water in earlier stage; Change quantity of exchanged water 50% of water every day in later stage; Temperature of fry nursing pool is 25 ℃～27 ℃ during the larvae cultivation; Salinity 28 ‰～32 ‰, the continuous conventional blowing aeration of cultivating process, as the larvae grow progressively increases the oxygenation amount makes water surface be the bubbling shape.
6, larval metamorphosis adheres to: under the situation that water temperature is 25 ℃～27 ℃; This batch of picture-weaving in silk Ba Fei clam larva was through 11 days artificial culture; Shell length can reach 280 microns, and at this moment the larva face has begun atrophy, and visible bar-shaped foot is intermarginal flexible at two shells; Receive seedling with 150 purpose bolting silk mesh bags this moment, and the nursery pond of putting into collected larva the adherance that is prepared in advance impels it to adhere to.Adherance 42 strings are thrown in every pond.
7, juvenile mollusk is cultivated: the spat after metamorphosis is adhered to continues to cultivate the entering juvenile mollusk stage under the condition of 26 ℃～29 ℃ of water temperatures; Cultivate through 9 days juvenile mollusks; Seed shell length can reach more than the 1mm, and naked eyes can be observed picture-weaving in silk line shape on its shell, again through 12 days cultivation; Most of juvenile mollusk shell reaches 3mm, begins to fall down from attachment flaps to fall into the nursery pond bottom.The juvenile mollusk nurturing period keeps the water quality of nursery pond good, nursery pond flowing water every day 2 hours, every day the twice unit cell algae bait of throwing something and feeding.Feeding volume is per 1,000,000 spats unit cell algae bait 12,000,000,000 cells of throwing something and feeding.The juvenile mollusk nurturing period is strengthened oxygenation intensity, and the pond water surface is the boiling shape.This is grown seedlings and successfully cultivates the picture-weaving in silk Ba Fei clam artificial seed 1,850 ten thousand of 3mm left and right sides specification through above-mentioned steps.
Present embodiment parent shellfish culture pond specification 4.0m*3.0m*1.0m, spawning pond specification 4.0m*4.0m*1.2m, nursery pond specification 4.0m*5.0m*1.2m.
Claims (1)
1. the artificial raise seedling method of picture-weaving in silk Ba Fei clam is characterized in that said method comprising the steps of:
(1) parent is strengthened: adopt and catch the natural waters ripe close shellfish in 2～3 ages; The back of returning is carried out body surface with the potassium permanganate sea water solution of 10ppm and is sterilized and be placed on preprepared close shellfish culture pond, and breeding density is placed 1～3 kilogram of close shellfish by every square of floor space, 50～100 centimetres of the close shellfish culture pond depth of waters; Ensure that the condition of water quality of close shellfish culture pond is 18～30 ℃ of water temperatures between parent shellfish culture period; Subsequent use close shellfish culture pond is prepared in seawater salinity 25 ‰～33 ‰, moves the pond every day once; Guarantee the nutrition supply of close shellfish; The high-quality of throwing something and feeding every day unit cell algae bait, feeding volume are per 5 kilograms of close shellfishes unit cell algae bait 30～10,000,000,000 cells of throwing something and feeding every day, and sampling is regularly dissected and carried out gonad development follow-up;
(2) artificial induced spawning: after gonad development gets into draining period, move into the temperature control case of hastening parturition through the close shellfish of above-mentioned cultivation and carry out artificial induced spawning through heating and cooling control; At first put into close shellfish the case of hastening parturition of 18～25 ℃ of water temperatures; 1～5 hour duration; Move into close shellfish the case of hastening parturition of 28～33 ℃ of water temperatures again, 0.5～2 hour duration, under the situation of close shellfish gonad maturity 1～3 time so repeatedly; The parent shellfish begins to arrange essence successively and lays eggs; The smart initial stage of laying eggs of close shellfish row through observing the smoke-like or the graininess of effluent, distinguish the male and female of close shellfish, by the close shellfish 10-14 of male and female: 1 ratio is evenly placed close shellfish and is gone into spawning pond; The overall control of each spawning pond parent shellfish is at 5～10 kilograms; The spawning pond water capacity is 10～25 cubic metres, and the condition of water quality of spawning pond is 25～30 ℃ of water temperatures, salinity 25 ‰～33 ‰, pH 7.8～8.3, DO＞6.5, COD＜1.5mg/L, content of nitrite＜0.15 μ g/L, hydrogen sulfide content＜0.05mg/L, and the close shellfish in the spawning pond was arranged essence successively and lays eggs and accomplish fertilization in 1～2 hour;
(3) artificial incubation: the fertilized egg of picture-weaving in silk Ba Fei clam can directly be hatched at spawning pond; 25 ℃～29 ℃ of control hatching water temperatures by the density of 3-10 fertilized egg of every milliliter of seawater cloth, keep the hatching water body to be in the fine motion state that emits minute bubbles through the Roots blower inflation; 25-50 minute fertilized egg begins division behind the insemination; Got into multicellular stage in 3-6 hour, 7-11 hour begins to rotate, and 12-18 hour develops into straight hinge larvas is " D " type larva;
(4) D type larva selected excellent: through 10～24 hours hatching process; The larva that those are second-rate and grow the fertilized egg go down and all can sink to the middle and lower part, pond; Adopt this moment and fish for or the method for siphon; Select for use 350 purpose bolting silk nets to drag for or mesh bag collects the high-quality young on top, put into ready nursery pond and cultivate;
(5) larvae cultivation: in nursery pond, cultivate by cultivating density the early stage of per 10 cubic metres of seawater cloth picture-weaving in silk Ba Fei clam larvas 300～8,000,000; Cultivating omnidistance the use is unit cell algae bait; Sooner or later the unit cell algae bait of throwing something and feeding every day twice, feeding volume are per 1,000,000 spats unit cell algae bait 50～10,000,000,000 cells of throwing something and feeding, and cultivate after 2～5 days to carry out 2 times every day and change water; Make the ocean temperature of cultivating in the pond keep 22～30 ℃; Salinity 25 ‰～28 ‰, the continuous conventional blowing aeration of cultivating process reaches more than 7 cultivation pond dissolved oxygen in water amount;
(6) larval metamorphosis adheres to: under the abundant condition of water temperature 25-28 ℃, bait; Picture-weaving in silk Ba Fei clam larva was through 8 days artificial culture, and shell length can reach 280～300 microns, and at this moment the larva face has begun atrophy; It is thus clear that bar-shaped foot is intermarginal flexible at two shells; Receive seedling with 150 purpose bolting silk mesh bags this moment, and the nursery pond of putting into collected larva the adherance that is prepared in advance impels it to adhere to, and it is a kind of plastic tab string that picture-weaving in silk Ba Fei clam larval metamorphosis adheres to employed adherance; Every string has 10-20 sheet rule or irregular plastic tab, and adherance 30～50 strings are thrown in every pond;
(7) juvenile mollusk is cultivated: the spat after metamorphosis is adhered to continues cultivation and has promptly got into the juvenile mollusk breeding phase, and the juvenile mollusk nurturing period keeps the water quality of nursery pond good, and nursery pond was wanted flowing water 2～5 hours every day; Every day the twice unit cell algae bait of throwing something and feeding; Feeding volume is per 1,000,000 spats unit cell algae bait 100～15,000,000,000 cells of throwing something and feeding, and the oxygen content of juvenile mollusk nurturing period larval rearing water is 6.8～8.1, cultivates through 7～10 days juvenile mollusks; Seed shell length can reach more than the 1mm; This moment, naked eyes on its shell can be observed picture-weaving in silk line shape, and through 10～15 days cultivation, the juvenile mollusk shell reached about 3mm again; Major part begins to fall down from attachment flaps and falls into nursery pond bottom, and this moment, spat was bred;
Said unit cell algae bait refer to marine green alga, chlorella, Isochrysis galbana, Dicrateria inornata or the Chaetoceros muelleri of gathering through artificial enlarged culture after introducing a fine variety, protect kind a kind of or combination in any contain algae liquid.
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| CN103125415A (en) * | 2013-03-19 | 2013-06-05 | 广西壮族自治区海洋研究所 | Artificial breeding method of tapes dorsatus |
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| CN105052792A (en) * | 2015-08-10 | 2015-11-18 | 苏州市相城区阳澄湖镇剑成水产生态养殖专业合作社 | High-yield breeding method for clams |
| CN106577414A (en) * | 2016-12-07 | 2017-04-26 | 广西壮族自治区海洋研究所 | Sea area artificial suspended breeding method of paphis textile |
| CN110839571A (en) * | 2019-12-13 | 2020-02-28 | 福建省洋泽海洋生物科技有限公司 | Artificial breeding method of red worms |
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| CN103125415B (en) * | 2013-03-19 | 2014-08-06 | 广西壮族自治区海洋研究所 | Artificial breeding method of tapes dorsatus |
| CN103782938A (en) * | 2014-01-26 | 2014-05-14 | 中国水产科学研究院南海水产研究所 | Method for acquiring parasitic polydora in pearl oyster shells |
| CN103782938B (en) * | 2014-01-26 | 2015-11-18 | 中国水产科学研究院南海水产研究所 | A kind of method obtaining pearl shell shell endoparasitism accomplished lady worm |
| CN105052792A (en) * | 2015-08-10 | 2015-11-18 | 苏州市相城区阳澄湖镇剑成水产生态养殖专业合作社 | High-yield breeding method for clams |
| CN106577414A (en) * | 2016-12-07 | 2017-04-26 | 广西壮族自治区海洋研究所 | Sea area artificial suspended breeding method of paphis textile |
| CN106577414B (en) * | 2016-12-07 | 2019-05-24 | 广西壮族自治区海洋研究所 | A kind of sea area of picture-weaving in silk Ba Fei clam manually hangs cultural method |
| CN110839571A (en) * | 2019-12-13 | 2020-02-28 | 福建省洋泽海洋生物科技有限公司 | Artificial breeding method of red worms |
| CN114586715A (en) * | 2022-03-24 | 2022-06-07 | 世倍(厦门)海洋科技有限公司 | Method for artificially breeding male fibraurea familiaris |
| CN114938788A (en) * | 2022-05-23 | 2022-08-26 | 广西海洋研究所有限责任公司 | Overwintering culture and fattening method for paphia textzle gmelin parent high-level pond |
| CN116267723A (en) * | 2022-12-26 | 2023-06-23 | 广西阿蚌丁海产科技有限公司 | Semi-artificial efficient breeding method for Ruditapes obtusifolia |
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