CN103782938A - Method for acquiring parasitic polydora in pearl oyster shells - Google Patents
Method for acquiring parasitic polydora in pearl oyster shells Download PDFInfo
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- CN103782938A CN103782938A CN201410038856.8A CN201410038856A CN103782938A CN 103782938 A CN103782938 A CN 103782938A CN 201410038856 A CN201410038856 A CN 201410038856A CN 103782938 A CN103782938 A CN 103782938A
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- worm
- accomplished lady
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention discloses a method for acquiring parasitic polydora in pearl oyster shells. The method comprises the following steps that (1) a pearl oyster with the parasitic polydora is selected, a shell body is opened, a polydora hole in the shell body is exposed out, and the polydora hole is obtained after pretreatment; (2) openings are formed in the two ends of the polydora hole respectively, anaesthetic liquid is injected into the openings, the polydora hole is completely opened after three to five minutes, and the polydora in the polydora hole is exposed out; (3) the polydora is taken out and arranged in a container containing anaesthetic antidotes, the polydora is made to weak up, the complete polydora individual is obtained, and the time from anaesthetic liquid injection to anesthesia dissolving is controlled between 10 min and 15 min. Polypide breakage or death caused by struggling of the polydora can be avoided, the polydora can be prevented from being accidentally hurt and misjudged, and the living and complete polydora can be acquired.
Description
Technical field
The present invention relates to a kind of method of obtaining pearl shell shell endoparasitism accomplished lady worm.
Background technology
Polydoraiosis is parasitosis common, the most very harmful in pearl breedinmg, can pierce in shell, forms the cave of various shapes, is commonly called as pearl shell " evil mind hepatopathy ".Because accomplished lady worm has extremely strong boring ability, can drill shell, invade pearl shell parenchymal tissue, so often make the pathologies such as the various organs of pearl shell are inflamed, atrophy, have a strong impact on growth and the growth of pearl shell, even cause pearl shell dead on a large scale.Live body feature is the important evidence of accomplished lady worm typoiogical classification, and the accomplished lady worm that obtains live body is also research accomplished lady worm habit, sets up the essential condition of larva culture technique.Complete polypide is the most basic condition of typoiogical classification, and any part that loses accomplished lady worm all likely causes classificatory mistake.So acquisition live body, complete accomplished lady worm all have great importance to Identification of Species and studies of the life histories.Accomplished lady worm is under the jurisdiction of Annelida polychaeta, is the polypide of a kind of softness, easy fracture, so, want to obtain complete accomplished lady worm and there is certain difficulty.And the accomplished lady worm of pearl shell parasitism is owing to having the hole habit of dwelling, the difficulty that the soft easily broken characteristic of the protection of hard shell and polypide itself makes to obtain complete live body accomplished lady worm is larger.
Summary of the invention
The object of the invention is to a kind of method of obtaining pearl shell shell endoparasitism accomplished lady worm, the polypide fracture that the method can be avoided causing due to the struggle of accomplished lady worm or dead, or accidental injury, wrong diagnosis polypide, can obtain accomplished lady worm live body, complete.
Above-mentioned purpose of the present invention is achieved through the following technical solutions: a kind of method of obtaining pearl shell shell endoparasitism accomplished lady worm, contains following steps:
(1) choose the pearl shell with parasitic accomplished lady worm, open housing, expose the accomplished lady worm cave on housing, after pretreatment, obtain accomplished lady worm cave;
(2) respectively establish an opening at two ends, accomplished lady worm cave, inject anesthesia liquid from opening part, wait for after 3～5 minutes, open accomplished lady worm cave completely, expose the accomplished lady worm in cave;
(3) accomplished lady worm is taken out, be placed in to be equipped with and separate narcotic container, accomplished lady worm is revived, obtain complete accomplished lady worm individuality, wherein, from injecting anesthesia liquid to starting to separate anesthesia, the time is controlled at 10～15min.
Pretreatment described in step of the present invention (1) comprises the pearl shell internal organ of removing accomplished lady worm cave periphery, and adopts filtering sea to clean.
Described in step of the present invention (2), the length of opening is preferably 0.5～1.5cm.
Described in step of the present invention (2), anesthesia liquid is magnesium chloride Mg Cl
2with the distilled water solution of sodium chloride nacl, wherein magnesium chloride Mg Cl
2concentration be 0.2～0.4mol/L, the concentration of sodium chloride nacl is 0.11～0.14mol/L.
Magnesium chloride Mg Cl of the present invention
2concentration be 0.3mol/L, the concentration of sodium chloride nacl is 0.12mol/L.
The consumption that the each opening of the present invention injects anesthesia liquid is 10～15 μ L.
Solution anaesthetic described in step of the present invention (3) is filtering sea.
Compared with prior art, tool of the present invention has the following advantages:
(1) prior art is not used anaesthetic, separates accomplished lady worm but directly choose the cave that punches, and the ratio that obtains so complete accomplished lady worm is low, and length consuming time, and Fig. 5 is shown in by the schematic diagram of the incomplete accomplished lady worm obtaining; And the present invention uses anaesthetic first in the time of picking accomplished lady worm, can improve the percentage of head rice that obtains polypide, can shorten again the picking polypide time;
(2) the present patent application people has carried out exploration discovery to anesthesia duration and anaesthetic dosage, the MgCl with pure seawater as solvent configuration
2solution more easily causes polypide to react violent, struggle or anaesthetize and excessively causes death, and applies physiological saline (0.12mol/L NaCl solution) as solvent configuration MgCl
2solution has the following advantages: because accomplished lady's Eimeria is in seawater parasite, the physiological saline of fresh water configuration has certain anesthetic effect to it, but anesthetic effect is strong not; But, and if MgCl
2mixed configuration anaesthetic but can reach very good effect, and the effect of oozing of the grade of physiological saline can prevent the dehydration of accomplished lady worm simultaneously and extremely, also can alleviate the struggle of accomplished lady worm, the percentage of head rice of increase polypide, and polypide also has the higher rate of reviving.
Accompanying drawing explanation
Fig. 1 is the pearl shell with accomplished lady worm cave of choosing in the embodiment of the present invention 1, and wherein arrow indication is accomplished lady worm cave;
Fig. 2 is that in the embodiment of the present invention 1, accomplished lady worm head, afterbody stretch out the schematic diagram outside cave;
Fig. 3 is accomplished lady worm postanesthetic complete accomplished lady worm in cave in the embodiment of the present invention 1;
Fig. 4 separates postanesthetic complete accomplished lady worm in the embodiment of the present invention 1, this accomplished lady worm tentacle, afterbody are all complete; Wherein 1 is afterbody, and 2 is head;
Fig. 5 is the schematic diagram of incomplete accomplished lady worm, and the head of this accomplished lady worm is without tentacle, tail break.
Embodiment
The method of what the present embodiment provided obtain pearl shell shell endoparasitism accomplished lady worm, containing following steps:
(1) tear osculum with nipper plier one at pearl shell shell edge, screwdriver enters shell is pried open from osculum interpolation, carefully removes the internal organ on side, cave, and filtering sea is rinsed region, cave well, and the accomplished lady worm cave in pearl shell as shown in fig. 1;
(2) respectively cutting an osculum apart from 1cm place, two ends, cave with scalpel, choose out shell chip with dissecting needle, until two ends, cave are chosen out to the opening of the about 1cm of length, accomplished lady worm head, afterbody stretch out schematic diagram outside cave as shown in Figure 2;
(3) sampling gun is drawn 10～15 μ L anesthesia liquids, from cave, opening part is slowly injected into, 10～15 μ L are all injected at two ends, cave, after injecting anaesthetic, wait for 3～5min, at this moment polypide is in narcosis, can avoid its struggle and cause the phenomenons such as fracture, two ends are injected into anaesthetic 20～30 μ L altogether, wherein the consumption of anesthesia liquid is extremely important, if anesthesia liquid consumption is too much, can cause accomplished lady worm polypide can not revive and cause death, if anesthesia liquid consumption is very few, can not play enough anesthetic effects, can cause follow-up accomplished lady worm completely not take out timely, anesthesia duration is also very important, as too short in anesthesia duration, the anesthesia of accomplished lady worm polypide is not enough, can be moving, can not take out complete accomplished lady worm polypide, as long in anesthesia duration, can cause the death of accomplished lady worm polypide, can not take out the accomplished lady worm of live body, specifically see the following form shown in 1 about the research of anesthesia duration.
Wherein, anesthesia liquid is magnesium chloride Mg Cl
2with the distilled water solution of sodium chloride nacl, wherein magnesium chloride Mg Cl
2concentration be 0.3mol/L, the concentration of sodium chloride nacl is 0.12mol/L, process for preparation is: in 100mL distilled water, add MgCl
22.85g, adds Nacl0.7g, then autoclave sterilization, preserve stand-by.
Table 1 anesthesia duration experimental result
Experimental result in table 1 shows, anesthesia 1min can not make polypide completely motionless, and anaesthetic effect is not obvious, and after anesthesia 30min, and the polypide rate of reviving is but very low, therefore anesthesia duration should be controlled in 3～15 minutes, the best is in 3～5min.
(4) cutting osculum with scalpel apart from the edge of cave 1cm left and right, then choose out chip with dissecting needle, progressively choose to cave direction, until cave is opened completely, expose the accomplished lady worm in cave, in cave, postanesthetic complete accomplished lady worm sees shown in Fig. 3;
(5) with tweezers, accomplished lady worm is carefully gone out from cave inner clip, be placed on and be equipped with in the plate of separating anaesthetic (filtering sea), polypide can revive in 3～5min, and from injecting anesthesia liquid to starting to separate anesthesia, the time is controlled at 10～15min, from injecting anaesthetic to starting to separate anesthesia, the general time is controlled at 10min, can not exceed at most 15min, otherwise accomplished lady worm can not revive, separate postanesthetic complete accomplished lady worm as shown in Figure 4, this accomplished lady worm tentacle, afterbody are all complete; Wherein 1 is afterbody, and 2 is head; And while not adopting anaesthetic, as shown in Figure 5, the head of this accomplished lady worm is without tentacle for the schematic diagram of incomplete accomplished lady worm, tail break.
Wherein separating anaesthetic filtering sea obtains by the following method: be the filter membrane of 0.22 μ m by seawater by aperture with vacuum filtration pump, the seawater after filtration is solution anaesthetic.
The method of what the present embodiment provided obtain pearl shell shell endoparasitism accomplished lady worm, containing following steps:
(1) tear osculum with nipper plier one at pearl shell shell edge, screwdriver enters shell is pried open from osculum interpolation, carefully removes the internal organ on side, cave, and filtering sea is rinsed region, cave well;
(2) respectively cutting an osculum apart from 1cm place, two ends, cave with scalpel, choose out shell chip with dissecting needle, until two ends, cave are chosen out to the opening of the about 1cm of length;
(3) sampling gun is drawn 10～15 μ L anesthesia liquids, from cave, opening part is slowly injected into, 10～15 μ L are all injected at two ends, cave, after injecting anaesthetic, wait for 3～5min, at this moment polypide is in narcosis, can avoid its struggle and cause the phenomenons such as fracture, two ends are injected into anaesthetic 20～30 μ L altogether, wherein the consumption of anesthesia liquid is extremely important, if anesthesia liquid consumption is too much, can cause accomplished lady worm polypide can not revive and cause death, if anesthesia liquid consumption is very few, can not play enough anesthetic effects, can cause follow-up accomplished lady worm completely not take out timely, anesthesia duration is also very important, as too short in anesthesia duration, and the anesthesia of accomplished lady worm polypide is not enough, can move, and can not take out complete accomplished lady worm polypide, as long in anesthesia duration, can cause the death of accomplished lady worm polypide, the accomplished lady worm that can not take out live body,
Wherein, anesthesia liquid is magnesium chloride Mg Cl
2with the distilled water solution of sodium chloride nacl, wherein magnesium chloride Mg Cl
2concentration be 0.2mol/L, the concentration of sodium chloride nacl is 0.11mol/L.
(4) cut osculum with scalpel in the edge apart from about the 1cm of cave, then choose out chip with dissecting needle, progressively choose to cave direction, until cave is opened completely, expose the accomplished lady worm in cave;
(5) with tweezers, accomplished lady worm is carefully gone out from cave inner clip, be placed on and be equipped with in the plate of separating anaesthetic (filtering sea), polypide can revive in 3～5min, from injecting anesthesia liquid to starting to separate anesthesia, time is controlled at 10～15min, and from injecting anaesthetic to starting to separate anesthesia, the general time is controlled at 10min, can not exceed at most 15min, otherwise accomplished lady worm can not revive.
Wherein separating anaesthetic filtering sea obtains by the following method: be the filter membrane of 0.22 μ m by seawater by aperture with vacuum filtration pump, the seawater after filtration is solution anaesthetic.
Embodiment 3
The method of what the present embodiment provided obtain pearl shell shell endoparasitism accomplished lady worm, containing following steps:
(1) tear osculum with nipper plier one at pearl shell shell edge, screwdriver enters shell is pried open from osculum interpolation, carefully removes the internal organ on side, cave, and filtering sea is rinsed region, cave well;
(2) respectively cutting an osculum apart from 1cm place, two ends, cave with scalpel, choose out shell chip with dissecting needle, until two ends, cave are chosen out to the opening of the about 1cm of length;
(3) sampling gun is drawn 10～15 μ L anesthesia liquids, from cave, opening part is slowly injected into, 10～15 μ L are all injected at two ends, cave, after injecting anaesthetic, wait for 3～5min, at this moment polypide is in narcosis, can avoid its struggle and cause the phenomenons such as fracture, two ends are injected into anaesthetic 20～30 μ L altogether, wherein the consumption of anesthesia liquid is extremely important, if anesthesia liquid consumption is too much, can cause accomplished lady worm polypide can not revive and cause death, if anesthesia liquid consumption is very few, can not play enough anesthetic effects, can cause follow-up accomplished lady worm completely not take out timely, anesthesia duration is also very important, as too short in anesthesia duration, and the anesthesia of accomplished lady worm polypide is not enough, can move, and can not take out complete accomplished lady worm polypide, as long in anesthesia duration, can cause the death of accomplished lady worm polypide, the accomplished lady worm that can not take out live body,
Wherein, anesthesia liquid is magnesium chloride Mg Cl
2with the distilled water solution of sodium chloride nacl, wherein magnesium chloride Mg Cl
2concentration be 0.4mol/L, the concentration of sodium chloride nacl is 0.14mol/L.
(4) cut osculum with scalpel in the edge apart from about the 1cm of cave, then choose out chip with dissecting needle, progressively choose to cave direction, until cave is opened completely, expose the accomplished lady worm in cave;
(5) with tweezers, accomplished lady worm is carefully gone out from cave inner clip, be placed on and be equipped with in the plate of separating anaesthetic (filtering sea), polypide can revive in 3～5min, from injecting anesthesia liquid to starting to separate anesthesia, time is controlled at 10～15min, and from injecting anaesthetic to starting to separate anesthesia, the general time is controlled at 10min, can not exceed at most 15min, otherwise accomplished lady worm can not revive.
Wherein separating anaesthetic filtering sea obtains by the following method: be the filter membrane of 0.22 μ m by seawater by aperture with vacuum filtration pump, the seawater after filtration is solution anaesthetic.
Embodiment 4
As different from Example 3, wherein, anesthesia liquid is magnesium chloride Mg Cl
2with the distilled water solution of sodium chloride nacl, wherein magnesium chloride Mg Cl
2concentration be 0.1mol/L, the concentration of sodium chloride nacl is 0.12mol/L.
Embodiment 5
As different from Example 3, wherein, anesthesia liquid is magnesium chloride Mg Cl
2with the distilled water solution of sodium chloride nacl, wherein magnesium chloride Mg Cl
2concentration be 0.6mol/L, the concentration of sodium chloride nacl is 0.12mol/L.
Comparative examples 1
As different from Example 3, wherein, anesthesia liquid is magnesium chloride Mg Cl
2distilled water solution, wherein magnesium chloride Mg Cl
2concentration be 0.1mol/L.
Comparative examples 2
As different from Example 3, wherein, anesthesia liquid is magnesium chloride Mg Cl
2distilled water solution, wherein magnesium chloride Mg Cl
2concentration be 0.3mol/L.
Comparative examples 3
As different from Example 3, wherein, anesthesia liquid is magnesium chloride Mg Cl
2distilled water solution, wherein magnesium chloride Mg Cl
2concentration be 0.6mol/L.
Comparative examples 4
As different from Example 3, wherein, the distilled water solution that anesthesia liquid is sodium chloride nacl, wherein the concentration of sodium chloride nacl is 0.12mol/L.
Anaesthetic effect in above-described embodiment 1,4,5 and comparative examples 1-4 is as shown in Table 2 below:
The anaesthetic effect experimental result of table 2 embodiment 1,4,5 and comparative examples 1-4
Experimental result in table 2 shows, 0.12mol/L Nacl solution has certain anesthetic effect to polypide; 0.1mol/LMgCl
2solution anesthetic effect is not obvious, and polypide is movable violent in picking process, and the probability of obtaining complete polypide is little; 0.6mol/LMgCl
2solution anesthetic effect is obvious, but dripping the anaesthetic incipient stage finds, polypide reaction is fiercer than dripping former Chi Shui reaction, enters anesthesia phases-time also very short, and the rate of reviving obviously reduces than other concentration groups; 0.3mol/L MgCl
2solution has higher complete polypide rate and the rate of reviving, and is optimal concentration in experimental group; In addition, MgCl
2the anesthetic effect of-Nacl mixed liquor is totally all than independent use MgCl
2solution or Nacl solution will be got well, 0.12mol/L NaCl solution is the concentration of physiological saline, not only there is certain anesthetic effect, and there is the effect that maintains osmotic pressure, can avoid accomplished lady worm cell rupture, can not allow cell too absorb water or dehydration, can guarantee that so postanesthetic accomplished lady worm has the rate of better reviving.
The present invention can summarize with other the concrete form without prejudice to spirit of the present invention or principal character.The above embodiment of the present invention all can only think explanation of the present invention rather than restriction, and any trickle modification, equivalent variations and modification that every foundation essence technology of the present invention is done above embodiment, all belong in the scope of technical solution of the present invention.
Claims (7)
1. obtain a method for pearl shell shell endoparasitism accomplished lady worm, it is characterized in that containing following steps:
(1) choose the pearl shell with parasitic accomplished lady worm, open housing, expose the accomplished lady worm cave on housing, after pretreatment, obtain accomplished lady worm cave;
(2) respectively establish an opening at two ends, accomplished lady worm cave, inject anesthesia liquid from opening part, wait for after 3～5 minutes, open accomplished lady worm cave completely, expose the accomplished lady worm in cave;
(3) accomplished lady worm is taken out, be placed in to be equipped with and separate narcotic container, accomplished lady worm is revived, obtain complete accomplished lady worm individuality, wherein, from injecting anesthesia liquid to starting to separate anesthesia, the time is controlled at 10～15min.
2. the method for obtaining pearl shell shell endoparasitism accomplished lady worm according to claim 1, is characterized in that: the pretreatment described in step (1) comprises the pearl shell internal organ of removing accomplished lady worm cave periphery, and adopts filtering sea to clean.
3. the method for obtaining pearl shell shell endoparasitism accomplished lady worm according to claim 1, is characterized in that: described in step (2), the length of opening is 0.5～1.5cm.
4. the method for obtaining pearl shell shell endoparasitism accomplished lady worm according to claim 1, is characterized in that: described in step (2), anesthesia liquid is magnesium chloride Mg Cl
2with the distilled water solution of sodium chloride nacl, wherein magnesium chloride Mg Cl
2concentration be 0.2～0.4mol/L, the concentration of sodium chloride nacl is 0.11～0.14mol/L.
5. the method for obtaining pearl shell shell endoparasitism accomplished lady worm according to claim 4, is characterized in that: described magnesium chloride Mg Cl
2concentration be 0.3mol/L, the concentration of sodium chloride nacl is 0.12mol/L.
6. according to the method for obtaining pearl shell shell endoparasitism accomplished lady worm described in claim 4 or 5, it is characterized in that: the consumption that each opening injects anesthesia liquid is 10～15 μ L.
7. the method for obtaining pearl shell shell endoparasitism accomplished lady worm according to claim 1, is characterized in that: the solution anaesthetic described in step (3) is filtering sea.
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| CN201410038856.8A CN103782938B (en) | 2014-01-26 | 2014-01-26 | A kind of method obtaining pearl shell shell endoparasitism accomplished lady worm |
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|---|---|---|---|
| CN201410038856.8A CN103782938B (en) | 2014-01-26 | 2014-01-26 | A kind of method obtaining pearl shell shell endoparasitism accomplished lady worm |
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| CN103782938B CN103782938B (en) | 2015-11-18 |
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| CN110214728A (en) * | 2019-07-18 | 2019-09-10 | 鲁东大学 | A kind of solution for making oyster quickly open shell and method |
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