CN102899162A - Technology for preparing euphausia superba oil in low fluorine content - Google Patents
Technology for preparing euphausia superba oil in low fluorine content Download PDFInfo
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- CN102899162A CN102899162A CN2012104110957A CN201210411095A CN102899162A CN 102899162 A CN102899162 A CN 102899162A CN 2012104110957 A CN2012104110957 A CN 2012104110957A CN 201210411095 A CN201210411095 A CN 201210411095A CN 102899162 A CN102899162 A CN 102899162A
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- euphausia superba
- fluorine content
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Abstract
The invention discloses a technology for preparing euphausia superba oil in low fluorine content. The technology comprises the following steps of (1) taking dried euphausia superba, adding acetone, extracting for three times in one hour per each time, merging extraction liquids, rotationally evaporating and removing the acetone, and obtaining the euphausia superba oil; (2) filtering and obtaining euphausia superba slag after the last extraction in step (1), adding n-hexane into the euphausia superba slag, extracting for three times in one hour per each time, merging extraction liquids, rotationally evaporating and removing the n-hexane, and obtaining the euphausia superba oil; and (3) obtaining the euphausia superba oil in low fluorine content by merging the euphausia superba oils obtained in the step (1) and the step (2). According to the technology disclosed by the invention, the extraction technology of the euphausia superba oil is optimized, the production of the euphausia superba oil is high, the fluorine content in the obtained euphausia superba oil is low, the quality of the euphausia superba oil is increased, and contribution is made for developing relative euphausia superba oil products.
Description
Technical field
The present invention relates to a kind of technique for preparing the krill shrimp sauce of low fluorine content.
Background technology
Fluorine is as one of several few trace elements with dual threshold character, in proper range, to the construction of biological hard tissue and promote the aspects such as living organism grows to play the irreplaceable effect of other elements.Otherwise, too high or too low, all unfavorable to the growth of biology.The mean concns of fluorine is 1.30mg/kg in world ocean.Fluorine content is higher than terricole in the marine animal, yet however, the fluorine content in most marine organisms is still not high, average out to n * 10
-4%.But the fluorine content of krill is very high, reaches n * 10
-1%.
Although have biological and more than krill of rich fluorine ability in the ocean, krill is as the maximum Living marine resources of Southern Oceans and even the whole world, its reserves are estimated up to 10 * 10
8～20 * 10
8T, but just enrichment 0.4 * 10 of the krill of only living
6～1 * 10
6The t fluorine.
Press united states drug, food control office regulation, the fluorine content that human body is taken in can not surpass 100mg/kg, and the fluorochemical toxicity test shows, contains the NaF food feeding mouse of 100mg/kg, can affect the enzymic activity in the body, changes eubolism.Contain the food feeding of 1500mg/kgNaF, slow down growth, and 17% mortality ratio generation is arranged.NaF is increased to three weeks of 2000mg/kg feeding in food, and mortality ratio reaches 100%.For guaranteeing the human body the most suitable growth, the intake that fluorine should allow is decided to be 2.5mg/kgF.With this criterion calculation, a grownup eats the dry weight krill of the freezing storage about 1.5g every day, can not surpass 14 grams at most, and analytical results shows, the whole krill fluorine of freezing storage content has exceeded 20 times of human body permission intake, and the krill of obvious freezing storage can not be used for normal edible.
The fluorine that human body absorbs enters blood after mainly being absorbed by stomach, wherein 75% with albuminous combination, part participates in blood circulation with the fluorochemical form, and enters tissue, saliva and kidney.Excreted by kidney, all the other mainly are that ight soil is got rid of.
Lack fluorine influential to tooth, the fluorine concentration of enamel essence can be up to 11mg/100g.Fluorine helps the hard of tooth, prevents carious tooth.Scarce fluorine makes bone ilium generation pathology, osteoporosis, and the joint increases slightly, a series of Kaschin-Beck disease signs such as amyotrophy.Scarce fluorine causes hematopoietic disorder.
High fluorine is except influential to tooth, bone ilium, hemopoietic function, and high fluorine is influential to neural system.Long-term excessive absorption fluorine can cause animal brain cortex and cortex inferior segment demyelination and cerebellum purkinje's cell reduced number, and these damages may cause neuronic contact minimizing and synaptic function unusual, then has influence on the function of brain.Sun Zengrong etc. (drink water of high fluorine content mouse brain hippocampal gyrus tissue hyper-microstructure observes 2000) touch hemato encephalic barrier by hippocampus of mice recurrent nerve cell, nerve fiber, spinous process to long-term drink water of high fluorine content and are subject to remarkable damage.Wherein, the presynaptic active region increases, and some presynaptic membrane, synaptic cleft and postsynaptic membrane all combine together, and the Blood Brain Barrier (BBB) permeability main manifestations is astrocyte podocytic process oedema, the plastosome sex change.The damage of hemato encephalic barrier may further increase, and the accumulating of fluorine in the cerebral tissue more increased the weight of fluorine to the damaging action of brain.
Fluorine is studied and is found the impact of brain tissue metabolism, and the accumulation of fluorine in brain hindered antioxidative functional, thereby causes the oxidative damage of nervus centralis, and energy generation and conversion are obstructed in the cerebral tissue simultaneously, and then have influence on ion transport and the nerve conduction of brain cell membrane.Fluorosis also can impact brain cell membrane lipid metabolism, protein metabolism and NO are synthetic in addition.
Fluorosis is on the impact of behavior, and the behavior of man and animal mainly is subjected to the domination of higher nervous center, and fluorosis causes that the damage of central nervous system form and metabolism change, and will impact behavior to a certain extent.Research point out the people to the susceptibility of fluorine far above mouse, find also take mouse as experimental subjects that in recent years fluorine is influential to the brain function of mouse.Malongs etc. (impact that fluorine is grown mouse filial generation neurobehavioral, 1993) are after dying fluorine for pregnant mouse, and the plane righting reflex of the cub of institute mouse and negative taxis test compliance rate significantly are lower than control group.Wu Nanping etc. drink the water that contains the different concns fluorine for pregnant mouse, and the general anthropometic index no abnormality seen of newborn mouse changes as a result, but the behavioral development of high dose group newborn mouse presents slight delayed effect, and are especially more obvious on motion and coordination function impact.Sun Zengrong etc. (high fluoride water on the impact of cerebral functions of mice, 2000) fed the drink mouse 6 months with the fluorinated water of different concns (100,50,10mg/L), show through the shuttle box experiment, but the learning and memory ability of high fluoride water remarkably influenced mouse.
High fluorine is on the impact of organism endocrine hormonal readiness: research finds, the white mouse that grows up reduces by 40% to fluorine group plasma testosterone level than control group; Investigation is found, T4 content obviously descends in fluorosis lesion children's blood, TSH then significantly raises, rats with fluorosis hypophysis LH total volume and release rate all are starkly lower than control group under external static culture conditions, and the prompting chronic fluorosis has potential damaging action to hypophysis LH emiocytosis function.(high fluorine is on the impact of organism endocrine hormonal readiness for Chen Peizhong etc., 2004) the research discovery, high fluorine state can cause the damage of the internal secretion bodies of gland such as hypophysis, Tiroidina, sexual gland, affect endocrine function, and then cause body function unusual, and high fluorine can pass to filial generation by reproductive process to the impact of organism endocrine, and then causes the damage of filial generation.
High fluorine has just been noticed the toxic action of fluorine to male reproductive system on the impact of reproductive system as far back as the nineteen twenty-five people, think fluorine and sterile between definite contacting arranged.In recent years some studies show that, fluorine suppresses the antioxidant reductase vigor by promoting free-radical generating, and causing lipid peroxidation to strengthen is the main mechanism that fluorine causes the male reproductive system infringement.NO can react with superoxide anion as the endogenous free radical, generates a kind of strong oxidizer peroxinitrites (ONOOH), induces lipid peroxidation.Carbon monoxide (NO) can be induced lipid peroxidation by ONOOH as a kind of important messenger molecule in the body, again can be to lipid peroxidation generation blocking effect.There are some researches show, nitricoxide synthase in the rats with fluorosis testicular cell (NOS) vigor obviously increases.People have observed the effect of Sodium Fluoride to the rear cleaning human sperm of ejaculating, and behind the 20min, because gathering gradually of Sodium Fluoride increases, cause the sperm membrane damage, the lysosome activity decreased, and sperm head elongates, the acrosome disappearance, afterbody becomes round.These variations show, Sodium Fluoride can pass through the testis barrier, disturbs normospermic growth course.
Krill is as the Living marine resources of South Pole maximum, and people have great expectations of these Biological resources always can be as human available protein resource.But because the high fluorine content of krill is harmful to HUMAN HEALTH, thereby bring certain difficulty for the direct utilization of these Biological resources.Some research attempts to carry out immediately the shelling processing of bright shrimp aboard ship after fishing for, but the protein yield is low, the wasting of resources is large, transports back after the country that has now fishes for it and is processed into grease, and the mixture of shrimp shell and albumen is as feed etc.And still contain a certain amount of fluorine in the grease, and inquire into the low fluorine technique of krill grease, reduce the fluorine content of grease, improve its oil quality, be the gordian technique that krill utilizes.
Summary of the invention
For above-mentioned prior art, the invention provides a kind of technique for preparing the krill shrimp sauce of low fluorine content.
The present invention is achieved by the following technical solutions:
A kind of technique for preparing the krill shrimp sauce of low fluorine content, step is as follows:
(1) get dried krill (water content is about 8%～10%) 100g, add acetone 500～600ml, 20～35 ℃ of lower extractions at least 3 times are extracted 1h at every turn, every extraction once, filtration leaches dried krill; United extraction liquid (approximately 1300～1500ml), 45～55 ℃, rotary evaporation is removed acetone under-0.07～0.09Mpa, and (approximately 6～6.2g), recording wherein by standard GB/T/T2005.18-2003, fluorine content is 109.352～117.688ug to get shrimp sauce;
(2) step (1) is last filters to get the shrimp slag after extracting, and adds the normal hexane of 500～600ml in the shrimp slag, and 20～35 ℃ of lower extractions at least 3 times are extracted 1h at every turn, every extraction once, filtration leaches dried krill; United extraction liquid (approximately 1300～1500ml), 45～60 ℃ ,-0.07～-0.09Mpa under rotary evaporation remove normal hexane, get shrimp sauce 4.519～4.832g, surveying wherein, fluorine content is 293.694～295.342ug;
(3) step (1) gained shrimp sauce and step (2) gained shrimp sauce are merged, (approximately 10.71～10.832g), wherein fluorine content is about 403.723～411.382ug namely to get the krill shrimp sauce of low fluorine content.
The optimised process that draws after many extraction processes that the present invention has been experimental study, such as: dried krill extracts with normal hexane, gets shrimp sauce 3.55g, and wherein fluorine content is 2346.290ug.Shrimp slag after normal hexane extracts is used acetone extraction again, gets shrimp sauce 3.88g, and fluorine content is 134.293ug.The mixing solutions of dried krill normal hexane: acetone=1:1 extracts, and gets shrimp sauce 6.6g, and wherein fluorine content is 718.674ug.Therefore select production technique of the present invention, not only oil extracting rate is high, and fluorine content is low in the oil.Why same dried krill raw material uses first acetone extraction grease productive rate high, because in the dried shrimp raw material moisture approximately 8～10%, water and oil all are dissolved in acetone, use first acetone extraction, removed the You Heshui in the raw material, can extract remaining lipid with normal hexane more on this basis, especially phosphatide etc., because normal hexane is not suitable for extracting grease from water-containing material, normal hexane can not contact with grease fully when moisture, so also just its dissolving can not be extracted.
The present invention has optimized the extraction process of krill shrimp sauce, and shrimp sauce output is high, and fluorine content is low in the resulting shrimp sauce, has improved the quality of shrimp sauce, has made contribution for developing relevant krill shrimp sauce product.
Embodiment
The present invention is further illustrated below in conjunction with embodiment.
The krill shrimp sauce of embodiment 1 preparation low fluorine content
Step is:
(1) dried krill 100g adds acetone 600ml, and 25 ℃ of lower extractions 3 times are extracted 1h at every turn, every extraction once, filtration leaches dried krill; Merge three times extracting solution (1500ml), 55 ℃ ,-0.09Mpa under rotary evaporation remove acetone, get shrimp sauce 6.26g, measuring wherein by standard GB/T/T2005.18-2003, fluorine content is 117.688ug.
(2) filter to get shrimp slag (85g) after the above-mentioned last extraction, add normal hexane 500ml in the shrimp slag, 25 ℃ of lower extractions 3 times are extracted 1h at every turn, every extraction once, filtration leaches dried krill; Merge three times extracting solution (1300ml), rotary evaporation is removed normal hexane under 45 ℃-0.09Mpa, gets shrimp sauce 4.519g, and after testing, fluorine content is 293.694ug in the oil.
(3) step (1) gained shrimp sauce and step (2) gained shrimp sauce are merged, namely get the krill shrimp sauce 10.779g of low fluorine content, fluorine content is 411.382ug.
The krill shrimp sauce of embodiment 2 preparation low fluorine contents
Step is:
(1) dried krill 100g adds acetone 550ml, and 35 ℃ of lower extractions 3 times are extracted 1h at every turn, every extraction once, filtration leaches dried krill; Merge three times extracting solution (1350ml), 45 ℃ ,-0.07Mpa under rotary evaporation remove acetone, get shrimp shrimp sauce 6.10g, surveying wherein by standard GB/T/T2005.18-2003, fluorine content is 113.240ug.
(2) filter to get shrimp slag (84g) after the above-mentioned last extraction, add normal hexane 550ml in the shrimp slag, 35 ℃ of lower extractions 3 times are extracted 1h at every turn, every extraction once, filtration leaches dried krill; Merge three times extracting solution (1360ml), 60 ℃ ,-0.07Mpa under rotary evaporation remove normal hexane, get shrimp sauce 4.610g, record that fluorine content is 295.342ug in the oil.
(3) step (1) gained shrimp sauce and step (2) gained shrimp sauce are merged, namely get the krill shrimp sauce 10.71g of low fluorine content.Fluorine content is 408.582ug.
The krill shrimp sauce of embodiment 3 preparation low fluorine contents
Step is: (1) dried krill 100g, add acetone 500ml, 20 ℃ of lower extractions 3 times are extracted 1h at every turn, every extraction once, filtration leaches dried krill; Merge three times extracting solution (1300ml), 50 ℃ ,-0.08Mpa under rotary evaporation remove acetone, get shrimp shrimp 6g, surveying wherein, fluorine content is 109.352ug.
(2) filter to get shrimp slag (86g) after the above-mentioned last extraction, add normal hexane 600ml in the shrimp slag, 20 ℃ of lower extractions 3 times are extracted 1h at every turn, every extraction once, filtration leaches dried krill; Merge three times extracting solution (1500ml), 55 ℃ ,-0.08Mpa under rotary evaporation remove normal hexane, get shrimp sauce 4.832g, record that fluorine content is 294.371ug in the oil.
(3) step (1) gained shrimp sauce and step (2) gained shrimp sauce are merged, namely get the krill shrimp sauce 10.832g of low fluorine content, fluorine content is 403.723ug.
Claims (1)
1. technique for preparing the krill shrimp sauce of low fluorine content, it is characterized in that: step is as follows:
(1) get dried krill 100g, add acetone 500～600ml, 20～35 ℃ of lower extractions at least 3 times are extracted 1h at every turn, every extraction once, filtration leaches dried krill; United extraction liquid, 45～55 ℃, rotary evaporation is removed acetone under-0.07～0.09Mpa, gets shrimp sauce;
(2) step (1) is last filters to get the shrimp slag after extracting, and adds the normal hexane of 500～600ml in the shrimp slag, and 20～35 ℃ of lower extractions at least 3 times are extracted 1h at every turn, every extraction once, filtration leaches dried krill; United extraction liquid, 45～60 ℃ ,-0.07～-0.09Mpa under rotary evaporation remove normal hexane, get shrimp sauce;
(3) step (1) gained shrimp sauce and step (2) gained shrimp sauce are merged, namely get the krill shrimp sauce of low fluorine content.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107904023A (en) * | 2017-12-21 | 2018-04-13 | 山东师范大学 | A kind of method that remaining grease is extracted in the dry shrimp slag from krill degreasing |
| CN107904009A (en) * | 2017-12-21 | 2018-04-13 | 山东师范大学 | A kind of method for extracting grease again from krill shrimp slag |
| CN113249170A (en) * | 2021-05-11 | 2021-08-13 | 大连理工大学 | Method for reducing acid value and fluorine content of antarctic krill oil |
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| CN1516592A (en) * | 2001-06-18 | 2004-07-28 | �����Ǽ���&������Դ����˾ | Krill and/or marine extracts for prevention and/or treatment of cardiovascular diseases, arthritis, skin cancers, diabetes, premenstrual syndrome and transdermal transport |
| CN102041166A (en) * | 2011-01-20 | 2011-05-04 | 山东师范大学 | Method for extracting krill oil with high phosphatide content from Antarctic krills |
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Patent Citations (2)
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| CN1516592A (en) * | 2001-06-18 | 2004-07-28 | �����Ǽ���&������Դ����˾ | Krill and/or marine extracts for prevention and/or treatment of cardiovascular diseases, arthritis, skin cancers, diabetes, premenstrual syndrome and transdermal transport |
| CN102041166A (en) * | 2011-01-20 | 2011-05-04 | 山东师范大学 | Method for extracting krill oil with high phosphatide content from Antarctic krills |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107904023A (en) * | 2017-12-21 | 2018-04-13 | 山东师范大学 | A kind of method that remaining grease is extracted in the dry shrimp slag from krill degreasing |
| CN107904009A (en) * | 2017-12-21 | 2018-04-13 | 山东师范大学 | A kind of method for extracting grease again from krill shrimp slag |
| CN107904023B (en) * | 2017-12-21 | 2021-01-08 | 山东师范大学 | Method for extracting residual grease from degreased dried shrimp residues of Antarctic krill |
| CN113249170A (en) * | 2021-05-11 | 2021-08-13 | 大连理工大学 | Method for reducing acid value and fluorine content of antarctic krill oil |
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