CN103564495B - Propolis and cordycepic hypha powder composition, as well as preparation and application thereof - Google Patents
Propolis and cordycepic hypha powder composition, as well as preparation and application thereof Download PDFInfo
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- CN103564495B CN103564495B CN201310540701.XA CN201310540701A CN103564495B CN 103564495 B CN103564495 B CN 103564495B CN 201310540701 A CN201310540701 A CN 201310540701A CN 103564495 B CN103564495 B CN 103564495B
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- propolis
- cordycepic
- caterpillar fungus
- chinese caterpillar
- hypha
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L21/00—Marmalades, jams, jellies or the like; Products from apiculture; Preparation or treatment thereof
- A23L21/20—Products from apiculture, e.g. royal jelly or pollen; Substitutes therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L31/00—Edible extracts or preparations of fungi; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Botany (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention provides a propolis and cordycepic hypha powder composition which comprises 45-60 percent of propolis, 30-45 percent of cordycepic hypha powder, 7-10 percent of maltodextrin. A preparation method of the composition comprises the steps of: respectively ultrasonically extracting a propolis raw material by using hydrous alcohol with different concentrations and purified water, mixing extracted filtrates, adding water in the cordycepic hypha powder according to a proportion for wetting the cordycepic hypha powder, uniformly mixing, uniformly adding the prepared propolis extracting solution in the prepared cordycepic hypha water solution by means of a magnetic stirrer, and preparing the propolis and cordycepic hypha powder composition by adopting methods of filtering mixed liquor, drying under a reduced pressure, freeze-drying and the like. According to the propolis and cordycepic hypha powder composition, the cell-mediated immunity and the body fluid immunologic function of an organism can be remarkably enhanced, and a synergistic effect is achieved in matching of the propolis and cordycepic hypha; in-vivo anti-aging experiments indicate that the propolis and cordycepic hypha powder composition has remarkable functions of resisting fatigues, oxidization, and aging, and improving the memory. Contents of main effective components of general flavone and polysaccharide of the propolis and cordycepic hypha powder composition are respectively limited, and the quality of the propolis and cordycepic hypha powder composition is conveniently controlled.
Description
Technical field
The invention belongs to field of health care food, relate to nourishing and health food category, particularly a kind of propolis Chinese caterpillar fungus hypha health-care food composition prepared for raw material with propolis and mycelium powder of sinensis, and its preparation method and application.
Background technology
Propolis is honeybee herborization resin, and is mixed into a kind of aromatic substance that beeswax and other secretion processes.Now from propolis, find more than 20 classes, more than 500 kind of natural component, comprising the mineral element of more than 30 kinds of needed by human, 20 seed amino acids, tens of kinds of aromatic compounds, more than 100 kind of Flavonoid substances, abundant organic acid, terpenoid substance, vitamin, lignanoid etc. is also had to have bioactive natural component.Propolis taste bitter, acrid, cold, return spleen, stomach warp, have qi-restoratives weak, change turbid fat, only quench one's thirst, external application removing toxicity for detumescence, convergence myogenic, for body empty early void, hyperlipidemia, quench one's thirst, and have anti-inflammation, antiviral, antimycotic, get rid of toxin, enhancing immunity, softening blood vessel, purify the blood, improve the biological action widely such as microcirculation, anti-oxidant, antitumor, cytotoxin, local anaesthesia, promotion regeneration.
Chinese caterpillar fungus hypha utilizes to produce Cordyceps Militaris separating obtained fresh Cordyceps sinensis from Qinghai---peacilomyce hepiahi bacterium strain, and by Submerged fermentation, by the finished product that tunning filtration drying is made.Modern medicine study shows, cordyceps contains cordycepic acid about 7%, carbohydrate 28.9%, fat about 8.4%, protein about 25%, in fat, 82.2% is unrighted acid, in addition, still containing vitamin B12, ergot lipidol, hexose alcohol, alkaloid, vitamin B12, fat, albumen etc.Wherein amino acids, polysaccharide, cordycepic acid are its main functional component.Polysaccharide is most important, the compounds of physiologically active in Chinese caterpillar fungus, and being considered to Chinese caterpillar fungus has two-way immunoregulatory effective component, has the effects such as antitumor, anti-inflammatory, hypoglycemic, reducing blood lipid.Chinese caterpillar fungus property is sweet, Wen Ping, nontoxic, it is famous strengthening by means of tonics medicine, there is nourishing the lung and the kidney, relieving cough and reducing sputum, expansion tracheae, calm, antibacterium, the effect such as hypotensive, and there is antitumor, anti-oxidant, anti-inflammatory and improve the functions such as immunity of organism, the fatigue substances in muscle can be decomposed, improve locomitivity.
At present, propolis and mycelium powder of sinensis have developed the health food of various function separately all, the ethanol of this patent employing different proportion and pure water extract the active component in propolis and Chinese caterpillar fungus to greatest extent, simultaneously by propolis and cordyceps extract composite after, take full advantage of the flavonoids in propolis, the synergy of the polysaccharide compound in phenolic acid compound and cordyceps mycelia, prescription has yin-nourishing yang-tonifying, antifatigue, anti-oxidant, anti-ageing, the several functions such as auxiliary hyperglycemic, and it is fine and smooth to prepare gained compounded combination composition granule through this patent, soluble in water, active ingredient in propolis and cordyceps mycelia is more easily absorbed by the body.
Summary of the invention
The object of this invention is to provide a kind of propolis Chinese caterpillar fungus hypha health-care food composition, the present composition is made up of the raw material of following weight percent: propolis 45%-60%; Mycelium powder of sinensis 30-45%, maltodextrin 7-10%, each constituent content percentage sum should equal 100%.
Another object of the present invention is to provide the preparation method of described composition, be achieved through the following technical solutions: (1) is selected by above-mentioned weight percentage by described propolis raw material, hydrous ethanol with 95%, 75% and 50% and pure water distinguish ultrasonic extraction propolis according to the solid-liquid ratio of 1 ︰ 4, ultrasonic extraction time controling is at 30-45 minute, filtrate after extraction merges, and obtains propolis extract; (2) described raw material mycelium powder of sinensis pure water is extracted 2 times by solid-liquid ratio 1:10 ultrasonic wave, ultrasonic extraction time controling is at 30-45 minute, filter, filter residue presses solid-liquid ratio 1:4 and 1:4 difference ultrasonic-leaching once with 50% and 75% ethanol water respectively, filtrate after extraction merges, and drying under reduced pressure removing ethanol, obtains cordyceps mycelia Aqueous extracts, then the maltodextrin of mass ratio 7-10% is added, fully the obtained Chinese caterpillar fungus hypha maltodextrin aqueous solution of mixing; (3) by the effect of magnetic stirring apparatus, evenly joined by the propolis extract prepared in the Chinese caterpillar fungus hypha maltodextrin aqueous solution prepared, fully mixing 30 minutes, temperature controls at 40 degree; (4) methods such as mixed liquor filtration, drying under reduced pressure or freeze drying are obtained the dry thing of propolis Chinese caterpillar fungus hypha.In the dry thing of prepared propolis Chinese caterpillar fungus hypha, general flavone content is not less than 4.0%, and polysaccharide is not less than 6%, and adenosine is not less than 0.01%.
Another object of the present invention is to provide described composition and is preparing the application in propolis and Chinese caterpillar fungus hypha health products.Described health products, by dry for propolis Chinese caterpillar fungus hypha thing, add appropriate auxiliary material or solvent is made according to existing conventional method, and described health products formulation is tablet, capsule, granule, sustained release tablets, drops or oral liquid etc.
Water-soluble for propolis difficulty, nutritional labeling extracts not congruent deficiency, different proportion ethanol water extraction is adopted to get, and make full use of the water soluble ingredients such as the polysaccharide in cordyceps mycelia, preparation have nourishment for vitality, blood-enrich, anti-oxidant, strengthen the several functions such as body immunity and water-soluble strong propolis Chinese caterpillar fungus hypha health-care food composition.
The present invention has following beneficial effect compared with prior art: with propolis and mycelium powder of sinensis for primary raw material, through special processing process, remain raw material propolis and Cordyceps Militaris, products obtained therefrom good water solubility, is easy to absorption of human body comprehensively.Product can be made into multiple formulation; Not only complementary propolis and Chinese caterpillar fungus hypha nutritive effect separately, and worked in coordination with total effect, there is the several functions such as yin-nourishing yang-tonifying, antifatigue, anti-oxidant, anti-ageing, auxiliary hyperglycemic, long-term taking has no side effect, preventable disease, improve malnutritive and fatigable situation, there is the health care and medical functions that delay senility.Carry out pharmaceutical research to composition of the present invention, result shows: the present composition obviously can strengthen cellular immunity, the humoral immune function of body, and propolis and Chinese caterpillar fungus interworking have synergistic effect; In body Antisenility Experiment show this prescription have obvious antifatigue, anti-oxidant, improve memory and anti-aging effects.The content of present composition extract principle active component general flavone, polysaccharide is limited respectively, is convenient to the quality controlling product.
Detailed description of the invention
Below in conjunction with embodiment, the invention will be further described.Propolis Chinese caterpillar fungus hypha health-care food composition of the present invention is made up of the raw material of following weight percent: propolis 45%-60%; Mycelium powder of sinensis 30-45%, maltodextrin 7-10%.Each constituent content percentage sum should equal 100%.
embodiment 1
A) selected natural fresh propolis 550 grams, freezing, be ground into fine powder, be mixed into drinking pure water and edible alcohol the solution and pure water ultrasonic extraction propolis that alcohol content is 95%, 75%, 50% concentration respectively.The ratio of the extraction solution of propolis and variable concentrations alcohol content is all 1:4, and ultrasonic extraction time controling was at 40 minutes, and respectively after Separation of Solid and Liquid, merging filtrate, obtains propolis extract.
B) selected mycelium powder of sinensis 350 grams, pure water is added by solid-liquid ratio 1:10, ultrasonic extraction time controling, at 30 minutes, filters, and it is each once that filter residue presses each ultrasonic-leaching of 1:4 volume ratio with 50% and 75% ethanol water respectively, merging filtrate, drying under reduced pressure removing ethanol, obtains Chinese caterpillar fungus hypha Aqueous extracts, then adds 100 grams of maltodextrins, abundant mixing, obtains the Chinese caterpillar fungus hypha maltodextrin aqueous solution.
C) propolis extract (a) prepared evenly joins in the Chinese caterpillar fungus hypha maltodextrin aqueous solution prepared under magnetic agitation effect, fully mixing 30 minutes, and temperature controls at 40 degree.
D) by the above-mentioned reacted propolis Chinese caterpillar fungus hypha aqueous solution after filtration, concentrated, dry obtained product.
E) propolis and Chinese caterpillar fungus are obtained as stated above, be pulverized and mixed evenly, add auxiliary material and mix, granulate according to a conventional method, dry, compressing tablet, dressing.
embodiment 2
A) selected natural fresh propolis 500 grams, freezing, be ground into fine powder, be mixed into drinking pure water and edible alcohol the solution and pure water ultrasonic extraction propolis that alcohol content is 95%, 75%, 50% concentration respectively.The ratio of the extraction solution of propolis and variable concentrations alcohol content is all 1:4, and ultrasonic extraction time controling was at 40 minutes, and respectively after Separation of Solid and Liquid, merging filtrate, obtains propolis extract.
B) selected mycelium powder of sinensis 400 grams, pure water is added by solid-liquid ratio 1:10, ultrasonic extraction time controling, at 45 minutes, filters, and filter residue is each once in each ultrasonic-leaching of 1:4 ratio with 50% and 75% ethanol water respectively, merging filtrate, drying under reduced pressure removing ethanol, obtains Chinese caterpillar fungus hypha Aqueous extracts, then adds 100 grams of maltodextrins, abundant mixing, obtains the Chinese caterpillar fungus hypha maltodextrin aqueous solution.
C) propolis extract (a) prepared evenly joins in the Chinese caterpillar fungus hypha aqueous solution prepared under magnetic agitation effect, fully mixing 30 minutes, and temperature controls at 40 degree.
D) by the above-mentioned reacted propolis Chinese caterpillar fungus hypha aqueous solution after filtration, concentrated, dry obtained product.
E) propolis and Chinese caterpillar fungus are obtained as stated above, be pulverized and mixed evenly, propolis Chinese caterpillar fungus hypha xeraphium is dissolved in water by 1:3 amount, adds appropriate amount of auxiliary materials, prepare oral liquid according to a conventional method.
embodiment 3on the impact of immunologic function
Preparation by reagent: selected natural fresh propolis 600 grams, freezing, be ground into fine powder, be mixed into drinking pure water and edible alcohol the solution and pure water ultrasonic extraction propolis that alcohol content is 95%, 75%, 50% concentration respectively.The ratio of the extraction solution of propolis and variable concentrations alcohol content is all 1:4, ultrasonic extraction 40 minutes, and respectively after Separation of Solid and Liquid, merging filtrate, obtains propolis extract.
Selected mycelium powder of sinensis 330 grams, pure water is added by solid-liquid ratio 1:10, ultrasonic extraction time controling, at 30 minutes, filters, and filter residue is each once in each ultrasonic-leaching of 1:4 ratio with 50% and 75% ethanol water respectively, merging filtrate, drying under reduced pressure removing ethanol, obtains Chinese caterpillar fungus hypha Aqueous extracts, then adds 70 grams of maltodextrins, abundant mixing, obtains the Chinese caterpillar fungus hypha maltodextrin aqueous solution.
Evenly joined under magnetic agitation effect by the propolis extract prepared in the Chinese caterpillar fungus hypha maltodextrin aqueous solution prepared, fully mix, temperature controls at 40 degree.By the propolis Chinese caterpillar fungus hypha aqueous solution after filtration, freeze drying obtains propolis mycelium powder of sinensis.
Preparation by reagent: propolis mycelium powder of sinensis distilled water is mixed with 1.25g/mL, 2.5g/mL, 5g/mL concentration.
Experimental design: three propolis Chinese caterpillar fungus dosage groups, a propolis control group and distilled water control groups are established in experiment.Basic, normal, high three propolis Chinese caterpillar fungus hypha dosage are respectively 0.5,1.0,2.0g/kg body weight, propolis control group dosage is bee glue powder 2.0g/kg body weight, distilled water control group gavage every day distilled water 20mL/kg body weight.
Animal used as test: ICR mouse, female, cleaning grade, body weight 18 ~ 22g
Experimental technique:
1. mice serum hemolysin test: respectively group gavage is to sample, every day 1 time, continuous 10 days.Test the 6th day, every mouse lumbar injection 0.2mL2%(V/V) hematocrit sheep red blood cell (SRBC) suspension, carries out immunity.After 4 days, get blood centrifugal, collect serum, with physiological saline by serum doubling dilution, 37 degree incubations 3 hours, observation hemagglutination degree, calculating antibody product.
2. the impact of Phagocytosis By The Peritoneal Macrophages In Mice: respectively group gavage is to sample, every day 1 time, continuous 30 days.First 40 minutes of sacrifice of animal, every mouse intraperitoneal injection 20 (V/V) chicken erythrocyte suspension 1ml, reinject after execution 2ml physiological saline, get abdominal cavity drop sheet, 37 DEG C of incubations 30 minutes, fixing dyeing, microscopy, counts 100 macrophages, calculates phagocytic rate and phagocytic index.
3. dinitrofluorobenzene inducing mouse delayed allergy test: each group gavage is to sample, and every day 1 time, continuous 30 days, test the 25th day, every mouse belly was coated with 50 μ l dinitrofluorobenzene solution (be coated with skin depilation in first 24 hours, area is about 3amX 3am).Within 5 days, be applied in mouse right ear with dinitrofluorobenzene solution 10 μ l afterwards to attack, within 24 hours, put to death animal, cut auricular concha, take off the auricle of diameter 8mm with card punch, weigh.
Experimental result:
1. affect mice serum hemolysin: compare with negative control group (distilled water), propolis and the basic, normal, high dosage group of propolis Chinese caterpillar fungus all can improve mice serum hemolytic antibody product level, propolis and propolis Chinese caterpillar fungus middle and high dosage group effect extremely remarkable.Also can find out from experimental result, propolis Chinese caterpillar fungus middle and high dosage group, than alone propolis dosage group more remarkable effect, illustrates that propolis and Chinese caterpillar fungus interworking have synergistic effect (table 1).
2. the impact of Phagocytosis By The Peritoneal Macrophages In Mice: compare with negative control group (distilled water), propolis and the basic, normal, high dosage group of propolis Chinese caterpillar fungus all can improve Phagocytosis By The Peritoneal Macrophages In Mice, and on propolis and propolis Chinese caterpillar fungus middle and high two, dosage group energy effect is extremely remarkable.Also can find out from experimental result, propolis Chinese caterpillar fungus middle and high dosage group, than alone propolis more remarkable effect, illustrates that propolis and Chinese caterpillar fungus interworking have synergistic effect (table 2).
3. the impact of dinitrofluorobenzene inducing mouse delayed allergy: compare with negative control group (distilled water), propolis and the basic, normal, high dosage group of propolis Chinese caterpillar fungus all can improve the delayed allergy of dinitrofluorobenzene inducing mouse, and on propolis and propolis Chinese caterpillar fungus middle and high two, dosage group energy effect is extremely remarkable.Also can find out from experimental result, propolis Chinese caterpillar fungus middle and high dosage group, than alone propolis more remarkable effect, illustrates that propolis and Chinese caterpillar fungus interworking have synergistic effect (table 3).
Conclusion:
Propolis and propolis cordycepin compound can strengthen the antibody-producting cell function of immunologic hypofunction mouse, improve hemolysin content, strengthen Function of mononuclear phagocyte, improve the delayed allergy of dinitrofluorobenzene inducing mouse, and propolis Chinese caterpillar fungus compound is better than propolis effect, illustrate that propolis and Chinese caterpillar fungus compatibility obviously can strengthen cellular immunity and the humoral immune function of body.
embodiment 4activity of fighting against senium in body
1. by the preparation of reagent: selected natural fresh propolis 520 grams, freezing, be ground into fine powder, be mixed into drinking pure water and edible alcohol the solution and pure water ultrasonic extraction propolis that alcohol content is 95%, 80%, 70%, 50% concentration respectively.The ratio of the extraction solution of propolis and variable concentrations alcohol content is all 1:4, ultrasonic extraction 40 minutes, respectively after Separation of Solid and Liquid, and merging filtrate.
Selected mycelium powder of sinensis 400 grams, pure water is added by solid-liquid ratio 1:10, ultrasonic extraction time controling, at 45 minutes, filters, and filter residue is respectively with 50% and 75% ethanol water in each ultrasonic-leaching of 1:4 ratio each 30 minutes, merging filtrate, drying under reduced pressure removing ethanol, obtains Chinese caterpillar fungus hypha Aqueous extracts, then adds 80 grams of maltodextrins, abundant mixing, obtains the Chinese caterpillar fungus hypha maltodextrin aqueous solution.
Evenly joined under magnetic agitation effect by the propolis extract prepared in the Chinese caterpillar fungus hypha maltodextrin aqueous solution prepared, fully mix, temperature controls below 40 degree.By the propolis Chinese caterpillar fungus hypha aqueous solution after filtration, freeze drying obtains propolis mycelium powder of sinensis.
2. grouping and modeling
Mouse is divided into 6 groups at random according to body weight: the basic, normal, high dosage group of normal group, model group, propolis group, propolis mycelium powder of sinensis group.Often organize 10, raise by 5 cages, normal diet.
Except normal group, the D-galactolipin normal saline solution of each group mouse neck in morning every day dorsal sc injection 0.15 g kg-1, normal group injection equal-volume physiological saline.After injection terminates, each administration group mouse is all by 0.1 mL 10g-1 body weight dose gavage, and dosage is: dosage group (0.5 g kg-1), propolis Chinese caterpillar fungus hypha high dose group (1 g kg-1) in propolis group (1 g kg-1), propolis Chinese caterpillar fungus hypha low dose group (0.1 g kg-1), propolis Chinese caterpillar fungus hypha.Normal group and model group mouse give equal-volume distilled water.
Observe the situations such as mouse activity, outward appearance, ight soil every day and record, according to body weight adjustment dosage, successive administration 56 d.
2.1 anti-ageing indexs
The activity of fighting against senium of propolis and propolis mycelium powder of sinensis optimized product characterizes with the aging of the outside of model mice and inside aging sign is weighed.Wherein, outside old and feeble sign comprises Body Mass Index and the behavioral indexes of mouse, and internal attribute comprises shoot formation and the Antioxidant Indexes of mouse.
2.2 Body Mass Index
Claim a body weight to for every 3 days each group of mouse, record and observe modeling situation, each mensuration before all selections are fed with the time morning is weighed.
2.3 behavioral indexes
Behavioral indexes comprises three parts: autonomic activities ability measures, step down test, mobility, the learning ability of examination mouse.
(1) autonomic activities ability measures
Within every two weeks, survey autonomic activities once.Under dark, micro-acoustic environment, mouse is put into respectively the independent activities case of autonomic activities instrument, wait for 1 min, make it to adapt to.Automatically record mouse active situation by computer after laundering period terminates, measure the movable number of times in every mouse 5 min.Each Simultaneously test is with 5 mouse of cage.
(2) learning ability test
Under dark, micro-acoustic environment, put into respectively by 5 of same cage mouse and independently measure case, mouse is shocked by electricity in bottom, jumps onto platform and can escape electric shock, and obtains memory.Old and feeble or amnemonic animal, ability of learning and memory within a certain period of time can decline, and jumping off number of times increases.Redeterminate after 24 h, record mouse is electrically shocked number of times lower mouse and jumps off the time (incubation period) of platform for the first time in 5 min, if mouse does not jump off from platform in 5 min, then calculates by 300 s incubation period.Duration of test carries out a diving tower test for every two weeks, observes the improvement result of each group of medicine to mouse aging non-space inquiry learning memory capability.
2.4 Antioxidant Indexes
Mouse orbit gets blood, centrifugal 10 min of 3000 rpm, separation of serum.The physiological saline that soma adds precooling according to w/v after weighing is prepared into the tissue homogenate of 10%, and centrifugal 15 min of 3000 rpm, get tissue homogenate supernatant and become desired concn with normal saline dilution again, and-80 DEG C of preservations are to be measured.
Measure mouse tissue protein content, serum and tissue SOD, GSH-px, CAT vigor, and the content of MDA in tissue.
3. experimental result
After mouse subcutaneous injection D-galactolipin, engender the situation that body weight increasess slowly.Within 4th week, start in modeling, compared with normal group, the body weight of model mice has started to present significant difference, and increases gradually with the prolongation difference of time, reaches pole significant difference to the 5th Zhou Shiyi.In modeling at the beginning of the 4th week, the Mouse Weight of propolis Chinese caterpillar fungus high dose group and model aging are organized mouse and are presented significant difference.And in last week of modeling, propolis Chinese caterpillar fungus hypha compound low dose group Mouse Weight still remains good ascendant trend, illustrate that the propolis Chinese caterpillar fungus hypha compound giving low dosage can intervene the situation of the Body weight loss of the mouse aging of D-galactolipin induction.
After mouse subcutaneous injection D-galactolipin, situations such as engendering that motion frequency declines, be slow in action, be slow in reacting.By measuring its autonomic activities ability discovery, the autonomic activities number of times in model group mouse 5min shows a process obviously declined for 2 weeks after modeling.When modeling 2 weeks, the autonomic activities frequency giving propolis group and propolis mycelium powder of sinensis basic, normal, high dosage group mouse produced significant difference with model group, and within the 6th week, expanded gap (table 4) further in modeling.Show that propolis and propolis mycelium powder of sinensis have the effect of the old and feeble mobility decline caused of opposing, and this effect is steady in a long-term.
Modeling the 4th week, model mice showed the diving tower errors number and the remarkable incubation period lower than normal mouse that are significantly higher than normal mouse.Show to compare normal mouse, model mice memory capability significantly declines, and possesses old and feeble outside and characterizes.Propolis and propolis Chinese caterpillar fungus hypha compound mouse show significantly lower than diving tower errors number and duration in the incubation period (table 5) being significantly higher than model mice of model mice in modeling on the 4th week.The performance of propolis Chinese caterpillar fungus hypha compound high dose group reaches extremely significant level of difference.Show that propolis Chinese caterpillar fungus hypha compound has the effect improving mouse aging non-NULL intersexuality Term Learning memory capability.
Show the result (table 6) of SOD vitality test in mice serum and tissue, model group mice serum SOD vigor significantly declines.Give propolis and propolis Chinese caterpillar fungus compound can significantly improve mice serum SOD vigor (p<0.05).Wherein, the performance of propolis Chinese caterpillar fungus high dose group is better than propolis control group, reaches pole significant difference (p<0.01), even compared with propolis group, also reach significant difference (p<0.05) compared with model group.
Model mice SOD in liver also significantly declines, and normal group has reached pole significant difference (p<0.01) by comparison.Give propolis, the middle and high dosage group of propolis Chinese caterpillar fungus can significantly improve SOD vigor (p<0.05) in Mice brain tissues.
The result of this research shows, propolis and propolis Chinese caterpillar fungus compound mixture all have stronger activity of fighting against senium in vivo, the situation of the Body weight loss of the mouse of D-galactolipin induction can be intervened, the situation of the mouse mobility decline of opposing D-galactolipin induction, improve mouse non-NULL intersexuality Term Learning memory capability, making it to keep the memory to stimulating, there is the learning ability of good avoidance harm, mice serum and SOD in liver can be improved.Therefore, propolis and propolis Chinese caterpillar fungus compound mixture can improve the outside of mouse and inside aging characterizes, and in propolis and Chinese caterpillar fungus compound formula, dosage group has been better than propolis group, and obvious synergistic effect is played in the performance of Chinese caterpillar fungus to propolis anti-senescence function.
Claims (4)
1. a propolis Chinese caterpillar fungus hypha health-care food composition, is characterized in that, is made up of the raw material of following weight percent: propolis 45%-60%; Mycelium powder of sinensis 30-45%, maltodextrin 7-10%, each material content percentage sum equals 100%; Described composition is prepared by the following technical programs: (1) percentage composition is selected by weight by propolis raw material, hydrous ethanol with 95%, 75% and 50% and pure water distinguish ultrasonic extraction propolis according to the solid-liquid ratio of 1:4, ultrasonic extraction time controling is at 30-45 minute, filtrate after extraction merges, and obtains propolis extract; (2) described raw material mycelium powder of sinensis pure water is extracted 2 times by solid-liquid ratio 1:10 ultrasonic wave, ultrasonic extraction time controling is at 30-45 minute, filter, filter residue presses solid-liquid ratio 1:4 difference ultrasonic-leaching once with 50% and 75% ethanol water respectively, filtrate after extraction merges, and drying under reduced pressure removing ethanol, obtains cordyceps mycelia Aqueous extracts, then the maltodextrin of mass ratio 7-10% is added, fully the obtained Chinese caterpillar fungus hypha maltodextrin aqueous solution of mixing; (3) by the effect of magnetic stirring apparatus, evenly joined by the propolis extract prepared in the Chinese caterpillar fungus hypha maltodextrin aqueous solution prepared, fully mixing 30 minutes, temperature controls at 40 degree; (4) mixed liquor filtered, obtain the dry thing of propolis Chinese caterpillar fungus hypha with drying under reduced pressure or freeze-drying method.
2. a kind of propolis Chinese caterpillar fungus hypha health-care food composition according to claim 1, is characterized in that, in the dry thing of propolis Chinese caterpillar fungus hypha prepared in preparation method, general flavone content is not less than 4.0%, and polysaccharide is not less than 6%, and adenosine is not less than 0.01%.
3. a kind of propolis Chinese caterpillar fungus hypha health-care food composition according to claim 1 is preparing the application in propolis Chinese caterpillar fungus hypha health products.
4. application according to claim 3, is characterized in that, described health products are that add appropriate auxiliary material and make, described formulation is tablet, capsule, granule, drops or oral liquid by dry for propolis Chinese caterpillar fungus hypha thing.
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