KR101351187B1 - Antioxidant and whitening functional cosmetics - Google Patents

Abstract

It has been known that seaweed sporophyl is effective in maintaining the body balance since it is enriched with minerals such as calcium, magnesium, iron, potassium and vitamins such as thiamine, riboflavin, niacin, and ascorbic acid. As the efficacy of the seaweed sporophyl is recently known, many research on the seaweed sporophyl are being conducted. The present invention is to provide cosmetics having anti-oxidizing, whitening, and moisturizing effects by extracting effective ingredients from seaweed sporphyl left in the sea. For achieving the purpose, the present invention provides a method for extracting anti-oxidant functional cosmetic raw material using seaweed sporophyl by performing a sample pulverizing procedure after measurement of moisture content, a yield measuring procedure and a biological activity measuring procedure after extraction according to elution conditions, filtration, and drying, and then a heavy metal analyzing procedure as a safety test of byproducts after extraction of final seaweed sporophyl, in order to establish optimal conditions for extracting components in the seaweed sporophyl. [Reference numerals] (140) Controller; (150) Alarm generator; (AA) Low-concentration odor; (BB) High-concentration odor

Description

Antioxidant Functional Cosmetics Raw Material Extraction Method Using Seaweed Ear and Antioxidant Functional Cosmetics Using the Same {Antioxidant and whitening functional cosmetics}

The present invention relates to a method for extracting antioxidant functional cosmetic raw materials using seaweed ears, and more particularly, by using the active ingredients of seaweed ears discarded in the sea as raw materials for cosmetics, to produce a cosmetic having an antioxidant effect, high quality, high added value Functional cosmetic raw material extraction method using seaweed ears that can be used as a material.

Seaweed is a brown algae, belongs to the seaweed family of kelp, and is native to the coast of Korea except for the region where the Korean wave and the eggs meet directly. Seaweed is a 1-year-old seaweed that grows on the rocks below the shipbuilding. It grows mainly from winter to spring, which is sporophyte of the asphyxus (2n), and sporophyte is released from sporangia formed in the late spring. Seaweed culture is carried out in mid-October after artificial seedling management, and it is attached to the mother line around mid-November and harvested after 4 ~ 5 months.

Seaweed is a 1-year-old seaweed, its attachment device branches into branches, and the end is like a sucker, so it can be attached to other objects firmly. The stem is flattened by the cylinder and is connected to the midrib of the upper leaf. When mature, the edges of the stem are greatly corrugated and turned into spindle (sporophyll). The leaf (upper part of the leaf) is deeply divided from the left and right, and many lobes are formed. As the plant grows, a new leaf-shaped part with lobes is formed in the growth zone at the contact area between the stem and the leaf part. Contains about 2% of fat, about 40% of carbohydrate, rich in calcium, potassium and magnesium, and also contains a large amount of iodine.

Seaweed is a seaweed that has been consumed for a long time as an important food for the role of seasoning as well as being used as a nutritive food to regain the health of mother and patient. However, leaves and stems are used through simple processing such as drying or salting. Seaweed powder juice, seaweed stem jam, and seaweed yangkang have been studied. Minerals (sporangium) are rich in minerals such as calcium, magnesium, iron, and potassium. Vitamins and dietary fiber such as thiamine, ribofuran, niacin, and ascorbic acid are also abundant and are known to be effective in maintaining body balance.

In addition, as the function of fucoidan, a physiologically active ingredient contained in trehalose, has been known, products such as health food and cosmetics utilizing a non-ferret have been developed. It has been reported that fucoidan is an acidic viscous polysaccharide contained in brown algae, seaweed, seaweed, and the like, and acts like antihistamine and blood purifying action due to its structure and action similar to heparin, an acidic polysaccharide having a strong anticoagulant action.

Fucoidan is also known to help eliminate cholesterol, lower blood cholesterol levels, prevent vascular disease, and prevent obesity from leading to adult disease. Research on anti-coagulant effect, antitumor action, gastric ulcer treatment stimulating action, antimicrobial action, inhibition of blood pressure increase, induction of hepatocyte growth factor (HGF) production, inhibition of hyperglycemia, antiallergic action and antiviral action at home and abroad Has been reported to have a 70-80% effect on the healing of cancers of the gastro-intestinal system and an excellent effect on most cancer healing. The properties of fucoidan are low in viscosity and excellent in solubility and are not only useful as water-soluble dietary fiber materials but also as feed additives for the prevention of viral diseases in fish and animals, as well as basic raw materials and additives for pharmaceuticals, functional foods and cosmetics It is a very high material.

Currently, in most seaweed farms in Korea, only harvesting leaves and shrimp and stalks are abandoned in the sea, which complaints are caused by secondary pollution such as pollution of the marine environment and fishing boats. Some parts of the shrimp are simply used as abalone food or edible food, but their use is insignificant, and there is a lack of industrialization of processing technology development and commercialization, so a more realistic industrialization direction is needed.

In order to solve such a problem, the present invention uses raw seaweed ear active ingredient as a raw material of cosmetics, raw material extraction of functional cosmetics having antioxidant activity using seaweed ear which can maintain elastic and healthy skin by the antioxidant effect through natural extracts. Provide a method.

Korean Patent No. 10-0879283 discloses a composition comprising 100 parts by weight of seaweed extract, 5 to 300 parts by weight of an undrawn extract, and 1 to 100 parts by weight of an extract of Namiliae mushroom, wherein the sea tangle extract, Or a mother liquor is soaked in water alone or mixed and then subjected to low-temperature extraction at a temperature of 10 to 50 DEG C for 5 to 100 hours to obtain a cosmetic composition comprising a seaweed extract. Korean Patent No. 10-1067648 discloses a method for extracting and concentrating kelp, and a method for extracting and concentrating chlorophyll from the residue by extracting with alcohol or ethanol, and adding the concentrate to the concentrate to enhance the functionality of chlorophyll The present invention relates to a method for producing chlorophyll-containing kelp concentrate by extracting and concentrating chlorophyll with respect to the extraction of the kelp powder, the method of producing the concentrate and the residue thereof. Korean Registered Patent No. 10-1084939 discloses a method for producing a granular product of 50 to 200 times (v / w) (RWF-1) by vacuum lyophilizing the supernatant obtained by centrifugation. The supernatant was removed from the supernatant obtained in the second step. (HWF-1) obtained by vacuum lyophilizing the supernatant obtained by centrifuging the extract at 50 to 150 DEG C for 30 minutes to 3 hours, And a method for preventing and treating hypertension. Korean Patent No. 10-1084939 discloses a method for producing a polysaccharide by hydrolyzing a marine spore leaf with a polysaccharide degrading enzyme; Preparing a low-viscosity enzyme digestion liquid by pressurizing and extracting the digestion product; Removing protein and alginic acid from the low-viscosity enzyme digestion solution to prepare a polysaccharide degradation solution; Decomposing and desalting polysaccharides in the polysaccharide degradation solution with an acidic solution to prepare a crude fucoidan solution; Disclosed is a sulfated fucose polysaccharide having anticoagulant activity from the seaweed pods comprising the step of collecting the crude fucoidan liquid by ion exchange chromatography, and fractionating the collection according to molecular weight It is. However, in the prior art as described in the present invention, by using the active ingredient of seaweed ear as a cosmetic raw material to give the antioxidant effect of the skin, it is possible to use the seaweed ear which is thrown away by the sea and interferes with marine pollution and fishery fishing, etc. It is different from the raw material extraction method of antioxidant functional cosmetics using seaweed ear which is effective in water.

The present invention is to solve the above problems, the seaweed extract with antioxidant effect can be used as a functional cosmetic raw material having a high value, by extracting the natural product as a raw material to minimize skin troubles and side effects and budding the skin The present invention provides an antioxidant functional cosmetic raw material extraction method using seaweed ears that can promote metabolism and maintain healthy skin, and an antioxidant functional cosmetic using the same.

The present invention as a means for solving the above problems is characterized by comprising the steps of: Measuring the moisture content and then pulverizing the sample; Adding the solvent to the pulverized soybeans to extract them; Filtering the extracted solvent; It provides an antioxidant functional cosmetic raw material extraction method using seaweed ears consisting of drying the filtered solvent.

In addition, the seaweed extract is characterized in that any one selected from water (Water), 10% by volume-ethanol, 20% by volume-ethanol or 30% by volume-ethanol as an extraction solvent, the seaweed extract is 20 to 100 ℃ It provides an antioxidant functional cosmetic raw material extraction method using seaweed ears, characterized in that used at the extraction temperature selected from.

The present invention opens the possibility of mass production and the creation of new income sources for fishing villages by using the seaweed extract as an antioxidant cosmetic raw material, by making the seaweed discarded in the sea into a cosmetic raw material that can be standardized into high quality and high value-added materials. It can reduce the damage caused by environmental pollution and fishing vessel fishing, and there are no harmful elements in the by-products generated after the extraction of dried seaweed and seaweed ears, so it can be used effectively in various materials such as food additives.

Fig. 1 is a photograph showing a shiso.
Figure 2 shows the optimal elution conditions of physiologically active components.
3 shows the water content of the hot-water extract powder.
Figure 4 shows the sugar content (Brix) of the extract powder.
Figure 5 shows the extraction yields for each solvent extract.
Figure 6 shows the extraction yields for extracts by temperature.
Figure 7 shows the extraction yield for the extracts over time.
Figure 8 shows the DPPH radical scavenging ability of the solvent extract.
Figure 9 shows the DPPH radical scavenging ability of extracts by temperature.
Figure 10 shows the DPPH radical scavenging ability of the extracts over time.
Fig. 11 shows the whitening activity of the solvent extract.
Fig. 12 shows the whitening activity of extracts by temperature.
Figure 13 shows the whitening activity of extracts over time.
14 shows heavy metal analysis of the hot-water extract.
Figure 15 shows a functional cosmetic raw material extraction method having an antioxidant effect using seaweed ears.
Figure 16 shows a functional cosmetics having an antioxidant effect using the seaweed ear prepared in the present invention.

Fig. 1 is a photograph showing a shiso. Minerals (sporangium) are rich in minerals such as calcium, magnesium, iron, and potassium, and are rich in vitamins such as thiamine, ribofuran, niacin, and ascorbic acid, which are known to be effective in maintaining body balance.

Raw material extraction method of the antioxidant functional cosmetics of the present invention comprises the steps of: a) grinding the selected sample by measuring the seaweed ear moisture content; b) extracting the solvent extract by adding a solvent to the ground seaweed and heating to a temperature of 20 to 100 ℃ for 2 to 24 hours; c) basic configuration is characterized by the step of filtering and drying the extracted solvent.

A method for extracting antioxidant functional cosmetic raw materials using a microflora comprises the steps of: a) pulverizing a selected sample by measuring the moisture content of the microfossils; b) extracting the solvent extract by adding water or 20% by volume ethanol as a solvent to the ground seaweed for 10 to 24 hours at a temperature of 60 to 100 ℃; c) filtering and drying the extracted solvent, the method for extracting whitening functional cosmetic raw materials using seaweed ears comprises the steps of: a) crushing the selected sample by measuring the moisture content of seaweed ears; b) extracting the solvent extract by adding water or 10 vol% -ethanol as a solvent to the ground seaweed and heating it at a temperature of 80 to 100 ° C. for 12 to 24 hours; c) filtering and drying the extracted solvent.

The cosmetic content of the seaweed extract having a whitening function is 250 to 1000 ug / ml, the cosmetic content of the seaweed extract having an antioxidant function may be made of 100 to 500 ug / ml.

Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings. In the following description of the present invention, detailed description of known functions and configurations incorporated herein will be omitted when it may make the subject matter of the present invention rather unclear.

1. Research Method

The experiment on the antioxidant effect of the solvent extraction and the extracted composition using seaweed ear was carried out as follows. Figure 2 shows a flow chart for the elution of the physiologically active elements of the gramineae.

1.1. Sample collection

The dried seaweed ears collected from the fishery were divided into Class A and Class B from the form and dried state, and 200g of each dried seaweed ears and 10kg of Class A dry seaweed ears for mass extraction were used after grinding the mixer.

1.2. Determination of optimal extract methods

In order to establish the optimum extraction conditions for the components in seaweed, heavy metals are measured as a safety test for sample by grinding water, extraction by elution condition, yield measurement after drying, measurement of antioxidant physiological activity, and other by-products after final seaweed extraction. Analysis was performed (FIG. 2).

1.2.1. Moisture content

The moisture content in the food was measured using an automatic moisture content meter (CEM, SMART system 5), and the mean and standard deviation were calculated after 5 repeated measurements.

1.2.2. Extraction conditions

Into a 250 ml round bottom flask, 10 g of dry seaweed sample was added with 100 ml of 10 times the extraction solvent (Water, 10% by volume-ethanol (EtOH), 20% by volume-EtOH, 30% by volume -EtOH) at each extraction temperature (20 Soxhlet extraction was carried out according to the ℃, 40 ℃, 60 ℃, 80 ℃, 100 ℃) and the extraction time (2-24 hours, every 2 hours). The extract was filtered (No. 7) and dried yield was measured.

1.3. Antioxidant effect (DPPH free radical scavenging activity)

After diluting the sample in DMSO, 150 μl of 10 mM DPPH (1,1-diphenyl-2-picrylhydrazyl) solution was added to each 50 μl of each extract by solvent, temperature, and time, vortexed for 10 seconds and then 1 ml of distilled water was added The absorbance was measured at 517 nm relative to the control (ascorbic acid) for 20 minutes.

1.4. Tyrosinase inhibition activity

40 μl of 0.1 M PBS, 80 μl of 1.5 mM tyrosine, and 40 μl of mushroom tyrosinase (2,000 units / ml) solution were added to each 40 μl of each extract by solvent, temperature, and time, vortexed for 10 seconds, The incubation was carried out at 37 DEG C for 20 minutes and absorbance was measured at 490 nm as compared with the control (Arbutin).

1.5. Analysis of heavy metals

After drying, remove 0.5 g each of dry soup and dried by-products in a 100 mL Erlenmeyer flask, mix 9 mL of 70% -HClO4 and 1 mL of H2SO4, dissolve in a 200 ℃ hot plate for 2 ~ 3 hours, Mercury, cadmium, and arsenic were analyzed by inductively coupled plasma emission spectrometer (Perkin Elmer, opima 7000DV).

2. Results

2.1. Moisture contents

3 shows the water content of the hot-water extract powder. There was no difference in the moisture content between the group A and the group B, and the difference between the two groups was not statistically significant.

2.2. Brix (%)

Figure 4 shows the sugar content (Brix) of the extract powder. The mean value of A and B group was 6.10 ± 0.04% and 5.87 ± 0.1%, respectively.

2.3. Extraction yield

In accordance with the method suggested in KFDA standardization process (manufacturing process standardization), the optimum extraction conditions according to the extraction solvent, extraction temperature, extraction time and drying method were carried out. Derived.

2.3.1. By extraction solvent

Figure 5 shows the extraction yields for each solvent extract. The extraction yield for each solvent extract was measured as 28.23 ± 5.62% of hot water extract, 32.03 ± 8.99% of 10% -EtOH extract, 35.57 ± 1.10% of 30% -EtOH extract and 39.30 ± 0.85% of 30% -EtOH extract, respectively. It became. 30 vol% -EtOH> 20 vol% -EtOH≥10 vol% -EtOH≥Hydrogen extract (0% -EtOH) showed the highest extraction yield in 30 vol% -EtOH, while EtOH extract below 20 vol% There was no statistically significant difference (FIG. 5).

KFDA's cosmetic extract solvents are water and less than 30% by volume of ethanol, and when considering the economic feasibility when extracting a large amount of solvents, it was judged that bulk hot water extraction is a method that is more safe and economical than ethanol extraction.

2.3.2. By extraction temperature

Figure 6 shows the extraction yields for extracts by temperature. The extraction yields by temperature of the solvent extraction were 11.77 ± 5.62% at 20 ℃, 11.80 ± 0.36% at 40 ℃, 11.85 ± 0.21% at 60 ℃, 14.95 ± 0.64% at 80 ℃ and 29.47 ± 1.72% at 100 ℃ And the extraction yield was the highest at 100 ° C (100 ° C> 80 ° C ≥60 ° C ≥40 ° C ≥20 ° C) (FIG. 6). As a result, it was confirmed that it is the most efficient method in hot water extraction at 100 ℃.

2.3.3. By extraction time

Figure 7 shows the extraction yield for the extracts over time. The extraction yields of the selected 100 ℃ hot water extraction over time were 2 hours 24.53 ± 4.03%, 4hour 30.47 ± 2.24%, 6hour 33.23 ± 8.53%, 8hour 42.66 ± 0.85%, 10hour 43.90 ± 0.85%, 12hour 45.10 ± 2.69% and 24hour 47.47 ± 2.28%, respectively, and 24hour ≥ 12hour ≥10hour ≥ 8hour ≥ 6hour> 4hour> 2hour.

Statistically, the extraction yields from 8 hours to 24 hours were significantly more significant than the extraction yields before 8 hours (FIG. 7). Therefore, considering the economical efficiency in mass extraction, it was confirmed that setting more than 6 hours, which is not significantly different from 8 hours, is the most effective method.

2.4. Antioxidant effect (DPPH free radical scavenging activity)

2.4.1. By extraction solvent

Figure 8 shows the DPPH radical scavenging ability of the solvent extract. The DPPH radical scavenging ability of the extract for each solvent was 44.38% of hot water, 10.vol.%-EtOH 14.50%, 20.vol.%-EtOH 52.60% and 30.%-EtOH 52.60% at 300µg / ml. Hot water at 53.71%, 10% by volume-36.65% by EtOH, 20% by volume-58.77% by EtOH and 62.03% by 30% by volume-EtOH, and 56.74%, 10% by volume -EtOH by 52.41% at 500 μg / ml 20 vol% -EtOH 66.42% and 30 vol% -EtOH 67.11%.

It was confirmed that the antioxidative effect was high in the hot water extraction with 20% -EtOH or more in the order of 30% -EtOH? 20% -EtOH? Hot water> 10% -EtOH on average in each concentration (Fig. 8). The results of the antioxidative effects of hydrothermal extracts showed that the extracts with 20% -EtOH or higher were more economical and safe than the extracts with 20% -EtOH.

2.4.2. By extraction temperature

Figure 9 shows the DPPH radical scavenging ability of extracts by temperature. The DPPH radical scavenging activity of the extracts was determined to be 10.09% at 20 캜, 26.78% at 40 캜, 44.36% at 60 캜, 47.15% at 80 캜 and 59.49% at 100 캜 at 100 / / 40 ° C., 59.53%, 60 ° C., 68.66% and 80 ° C. at 500 μg / ㎖ at 50 ° C and 43.70%, 40 ° C and 51.95% 69.21% and 100 캜 74.17%, respectively. The average concentration of each concentration was 100 ° C> 80 ° C ≥60 ° C ≥40 ° C> 20 ° C, and it was confirmed that the antioxidative effect was the highest at 100 ° C (FIG. 9).

2.4.3. By extraction time

Figure 10 shows the DPPH radical scavenging ability of the extracts over time. The DPPH radical scavenging activity of the selected 100 캜 hot water extract was measured to be 2.45% for 2hour, 42.59% for 4hour, 57.73% for 6hour, 58.20% for 8hour, 61.28% for 10hour, 65.12% for 12hour and 68.71% for 24hour , 2hour 54.95%, 4hour 54.30%, 6hour 58.75%, 8hour 59.19%, 10hour 66.56%, 12hour 67.11% and 24hour 69.47% at 300 ㎍ / , 6hour 59.06%, 8hour 60.01%, 10hour 68.86%, 12hour 71.72% and 24hour 72.60%, respectively.

On the average, 24hour ≥ 12hour ≥ 10hour> 8hour ≥ 6hour>4hour> 2hour were shown in order of concentration, indicating that the antioxidative effect was the highest after 10hour (FIG. 10). Considering the extraction yields over time and economical efficiency in mass production, it is considered effective to set the extraction time after 6 hours.

2.5. Tyrosinase inhibition activity

2.5.1. By extraction solvent

Fig. 11 shows the whitening activity of the solvent extract. As a result of measuring the whitening activity of the extract for each solvent, hot water 30.30%, 10 vol% -EtOH 25.62%, 20 vol% -EtOH 20.21% and 30 vol% -EtOH 19.08% at 250 µg / ml, and at 500 µg / ml Hot water 33.03%, 10% vol.-EtOH 29.71%, 20% vol.-EtOH 25.87%, and 30% vol.-EtOH 21.58%, 40.31%, 10vol.%-EtOH 31.80%, 20 at 1000 μg / ml. Measured by volume% -EtOH 29.11% and 30 volume% -EtOH 24.13%. On average for each concentration, hot water> 10% by volume EtOH> 20% by volume EtOH> 30% by volume EtOH showed the highest whitening activity of the hot water extract (FIG. 11).

2.5.2. By extraction temperature

Fig. 12 shows the whitening activity of extracts by temperature. The whitening activity of hot water extracts at different temperatures was measured at 250 / / ㎖ at 20 ℃ 13.19%, 40 ℃ 16.95%, 60 ℃ 20.73%, 80 ℃ 24.94% and 100 ℃ 28.82% 20.65% at 40 占 폚, 21.92% at 40 占 폚, 24.50% at 60 占 폚, 26.91% at 80 占 폚 at 1000 占 퐂 / ml, 16.46% at 40 占 폚, 17.18% at 40 占 폚, 20.80% at 60 占 폚, % And 100 < 0 > C 30.87%. It was confirmed that the whitening activity was the highest at 100 ° C in the order of 100 ° C> 80 ° C> 60 ° C> 40 ° C> 20 ° C for each concentration (FIG. 12).

2.5.3. By extraction time

Figure 13 shows the whitening activity of extracts over time. The whitening activity of the selected hot water extracts of 100 ℃ was measured at 250 ㎍ / ㎖, which was 18.43% for 2hour, 19.34% for 4hour, 19.84% for 6hour, 23.33% for 8hour, 24.50% for 10hour, 27.23% for 12hour and 29.05% for 24hour , 2hour 21.68% and 4hour 21.92% at 1000 ㎍ / ㎖, respectively. The concentration of 2hour was 20.26%, 4hour 20.43%, 8hour 23.66%, 10hour 25.40%, 12hour 28.60% and 24hour 29.95% , 6hour 24.50%, 8hour 25.75%, 10hour 26.75%, 12hour 30.87% and 24hour 35.78%, respectively.

On the average, 24hour>12hour>10hour>8hour>6hour>4hour> 2hour were observed in the order of each concentration, and the whitening activity was highest from 12hour onward. Statistically, there was a significant difference between before and after 8hour compared with after 12hour. This result can be deduced from the fact that the time of 6hour mentioned above does not show a significant difference considering the economical efficiency in mass extraction.

2.6. Analysis of heavy metals

14 shows heavy metal analysis of the hot-water extract. The concentration of heavy metals in the KFDA cosmetics heavy metals range (KRDA STD) is less than 1ppm for lead, mercury, cadmium and arsenic, and the heavy metal concentration of raw materiral is 0.007ppm for mercury, 0.004ppm for cadmium and 0.038ppm for arsenic . In addition, the concentration of heavy metals in the by-product of by-product was found to be at a trace level, which is lower than the permissible concentration range for both dry and non-dried by-product samples, with lead undetected, 0.016 ppm mercury, 0.002 ppm cadmium and 0.071 ppm arsenic Safety was proved as a cosmetic raw material (Fig. 14).

3. Optimal elution conditions

By grinding the seaweed ear, hot water extraction, extraction temperature 100 ^o C, extraction time 6hr, the number of extraction once and then lyophilized and tested as a cosmetic raw material containing an effective ingredient excellent in water content, antioxidant effect (Fig. 15).

A method for extracting antioxidant functional cosmetic raw materials using a microflora comprises the steps of: a) pulverizing a selected sample by measuring the moisture content of the microfossils; b) extracting the solvent extract by adding water or 20% by volume ethanol as a solvent to the ground seaweed for 10 to 24 hours at a temperature of 60 to 100 ℃; c) filtering and drying the extracted solvent.

4. Preparation of functional cosmetics with antioxidant effect using extract

Extraction time for seaweed extract As it was confirmed that it can be used as a raw material of functional cosmetics excellent in moisture content and antioxidant effect according to the temperature and solvent type of solvent, functional cosmetics excellent in antioxidant effect were prepared using the seaweed extract solvent of the present invention. It was.

Functional cosmetic content of the seaweed extract having a whitening function was to contain 250 to 1000 ug / ml seaweed ear solvent extract of the present invention, and the cosmetic content of the seaweed extract having an antioxidant function to contain 100 to 500 ug / ml. Other raw materials for cosmetics to be contained are generally prepared by mixing oil, water, surfactant, vitamins and the like as raw materials used for manufacturing cosmetics. Figure 16 shows the antioxidant functional cosmetics prepared in the present invention.

Antioxidant functional cosmetics prepared according to the present invention is a cleansing foam, skin lotion, emulsion, nutrition cream, essence, ampoule, eye cream, cleansing lotion, massage cream, peeling gel, men's skin lotion, peeling gel, men's emulsion, BB cream, slimming gel , High frequency gel, high frequency cream, hand disinfectant and the like, the method of use can generally be applied to the affected area can be used, depending on the dosage form can be selected the preferred method of administration.

The daily dose of the composition of the present invention is determined depending on the subject to be administered, the method of administration, and symptoms, and the administration frequency is preferably at least twice a day, but the administration frequency can also be adjusted depending on the degree.

Seaweed ear extract has excellent antioxidant effect and can produce high-quality cosmetics.In particular, even by-products generated after dry seaweed and seaweed extract have heavy metals below the KFDA's acceptable concentration. It is possible to produce foods containing them, and to use large amounts of seaweeds thrown away at sea, which can promote coastal fisheries environmental conservation and increase income of fishers.

Claims (5)

The sample selected by measuring the seaweed ear moisture content was pulverized, and 20 volume% ethanol or 30 volume% ethanol was added to the ground seaweed as an extraction solvent, and the extract was heated for 6 to 12 hours at a temperature of 60 to 100 ° C. Antioxidant functional cosmetics using Wakame ear extract, characterized in that 300 to 500 μg / ml of seaweed extract extracted by the antioxidant functional cosmetic raw material extraction method consisting of filtering and drying the delete delete delete delete

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