JP2007306898A - Functional food using cordyceps sinensis (berkeley) saccardo, and method for producing the same - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide functional food using Cordyceps sinensis (Berkeley) Saccardo, producible at relatively low cost, and expected to bring wider effect compared to a conventional product, and to provide a method for producing the functional food. <P>SOLUTION: This method for producing the functional food comprises (S1) adjusting a suspension of spores of sterile-cultured Isaria japonica IM2001 (FERMP-18253) strain, (S2) inoculating sterile-bred silkworm pupa as a host to the product, (S3) culturing the product in a sterilized condition to obtain Cordyceps sinensis (Berkeley) Saccardo, (S4 and S5) extracting active ingredients followed by milling or (S6) drying the product as it is followed by crushing treating. The method comprises, on the other hand, adding water to Aronia fruits followed by homogenizing with a Waring blender, stirring and extracting the product to obtain Aronia extract, and (S7) crushing treating the powdery product of Cordyceps sinensis (Berkeley) Saccardo and the Aronia extract followed by mixing with each other as first and second components, (S8) adding appropriate sugar to the product, and (S9) obtaining the functional food. <P>COPYRIGHT: (C)2008,JPO&INPIT

Description

  The present invention relates to a functional food mainly using cordyceps and a method for producing the same, and relates to an improved technique for obtaining effects such as recovery of eye strain in addition to the original effects of cordyceps.

  Cordyceps is a fungal fungus that belongs to the Ascomycetes ergotaceae, and is the fruiting body formed by parasitizing insects such as moths and moths, and the corpse of the host insect. Consists of a complex. Approximately 350 species are known in Japan, but the dried products are mainly produced in China, and have been used in the private sector for a long time as herbal medicines with effects such as tonicity, strength, longevity, nourishment and tonic. Has been.

Cordyceps is precious as a wild species, but in recent years it has been spotlighted as a functional food (supplement) with an action of removing active oxygen in addition to the above-mentioned effects. An artificial culture method that can obtain cordyceps in high efficiency and in a short period of time has been established by using a simple insect culture technique.
Japanese Patent Laid-Open No. 2005-239585 JP 2002-272267 A JP-A-2005-213242 JP 2001-172191 A JP 2004-267102 A

By the way, regarding the application of Cordyceps sinensis to functional foods, the days of research and development are still short, and it can be said that there is still room for improvement in order to obtain better efficacy.
For example, Cordyceps is originally considered to have a remarkably broad effect compared to other functional foods, including fatigue recovery and nourishing tonics, but there is still a bias in the effect of Cordyceps. For this reason, even in functional foods using cordyceps, there is room for improving the bias in efficacy.

For example, in recent years, there has been an increase in the number of people who complain of dry eye and eye strain due to the widespread use of OA equipment such as computers and the use of work during irregular hours. The effects of eye strain are considered to have a large effect on the entire body, including headaches and stiff shoulders. Solving problems directly and indirectly related to the eyes is important for maintaining the health of the entire body. It is.
However, it is said that Cordyceps sinensis has a relatively small effect on the eye compared to other effects at the present research stage. Therefore, even in functional foods using cordyceps, taking measures against eye strain is a very significant issue and should be taken as soon as possible.

  The present invention has been made in view of the above problems, and provides a functional food using cordyceps that can be produced at a relatively low cost and can be expected to have a wide range of effects compared to conventional products, and a method for producing the same. The purpose is to do.

In order to solve the above-mentioned problems, the present invention provides a composition comprising a first component obtained from Cordyceps sinensis including the moss mosquito Isaria japonica IM2001 (FERMP-18253) and its host, and a second component obtained from Aronia fruit. It was set as the functional food characterized by including.
Here, as a host in the cordyceps can use aseptically raised pupae. Such aseptically raised culm is highly advantageous in that it can completely avoid the adverse effects of various germs and can be obtained relatively easily by the sericulture system established in Japan and can use pure domestic materials. With reliability.

In addition, the composition can be in the form of a powder or a granule to facilitate the user's dose.
Furthermore, as said 1st component, the thing which extracted the dried material of the said Cordyceps sinensis under high temperature conditions with the organic solvent can be used.
On the other hand, the present invention is a mixing step of mixing the first component of Cordyceps sinensis obtained by inoculating the spore of Isaria japonica IM2001 (FERMP-18253) strain with the second component obtained from Aronia fruit. It was set as the manufacturing method of the functional food which passed through.

  Here, in the mixing step, the powdered first component and the second component can be mixed.

  According to the functional food of the present invention having the above-described configuration, firstly, the first component derived from Cordyceps sinensis has superior antioxidant activity (free radicals), such as hanasanagin (3,4-diguanidinobytanoyl-DOPA). Erase activity) is exhibited. As a result, it has effects such as nourishing tonic, anti-cancer, anti-allergy, anti-atopic dermatitis, anti-anginal, anti-arrhythmia, anti-arteriosclerosis, anti-asthma, antibacterial, anti-diabetic, detoxification, anti-inflammatory, anti-high The prevention, health, and therapeutic effects of lipemia are exhibited.

On the other hand, secondly, the second antioxidant component derived from the aronia fruit extract also has a good antioxidant effect, and thereby each effect based on the first component is exhibited synergistically. In addition, anthocyanins (polyphenols), which are abundant active ingredients unique to Aronia fruit species, can be expected to exert excellent effects on eye diseases.
In other words, polyphenols have the effect of promoting the resynthesis of rhodopsin that is decomposed by overuse of the retina of the eye, and the aronia fruit extract containing this component improves eye strain, improves visual acuity, dark adaptation, and stiff shoulders. Effective effects such as prevention and healthy stomach can be obtained.

By the combination of the first and second components described above, in the present invention, a very wide and stable effect is exhibited as compared with the conventional functional food mainly composed of cordyceps.
In the production process of the functional food of the present invention, the first component and the second component can be separately pulverized. However, if both components are mixed and pulverized at once, both components Can be mixed uniformly to ensure a stable quality, and in the process of pulverization, the first component and the second component interact with each other, and a synergistic effect may be exhibited.

  As described above, according to the present invention, the second component is used to supplement the efficacy of the functional food containing the Cordyceps sinensis component alone as the main component for problems such as eye strain that may not be sufficiently effective. Alternatively, it can be imparted by a further action. For this reason, as a whole functional food, a very wide and stable effect is exhibited, and a highly complete structure can be obtained.

  As used herein, the term “corticus” refers to the whole fruit body of the flower moth and its host moth.

Embodiments and examples of the present invention will be described below, but the present invention is naturally not limited to these forms, and may be appropriately modified and implemented without departing from the technical scope of the present invention. be able to.
<Embodiment 1>
(Composition of functional food)
FIG. 1 (a) is a schematic configuration diagram when the functional food in Embodiment 1 is enlarged with an optical microscope.

As shown in the figure, the functional food 1A is a powder, and is a powder 10A obtained by drying the first component obtained from Cordyceps sinensis and the second component obtained from the Aronia fruit extract. Are mixed with particles 11A obtained by drying, and further added with particles 12A of taste adjusting additives such as sugars.
The components per about 3 g of the functional food 1A are as shown below. This blending amount is not particularly limited and can be adjusted as appropriate. In the example of the first embodiment, the aronia fruit component is obtained by freeze-drying the fruit. However, in order to use the active ingredient in abundance, it is set to contain more than the Cordyceps component.

Cordyceps ingredient; 1.0g
Aronia fruit ingredient; 2.0g
Other (fructose); slightly

The functional food 1A having the above configuration is laminated and packed in a stick-shaped aluminum exterior film that is excellent in airtightness and preservability, for example, once to three times a day (about 3 g) as an example. Can be taken as it is after opening or with some moisture. Thereby, in addition to the effects of the effects of the first and second components described below, a synergistic effect of these two components is exhibited.

In addition, since the Aronia component contains a red pigment and has a sour flavor peculiar to berries, adding the Aronia component improves the flavor and mouthfeel of the functional food and makes it easier to take. Such effects are also achieved.
The functional food of the present invention is not limited to a powdery 1A structure, but as shown in FIG. 1 (b), the particles 10A to 12A are individually or mixed to a predetermined shape or size, and thereby Granules (here, cylindrical granules) 10B may be used. Specifically, it is possible to adjust the size of several μm or more and several mm or less. In this case, it can be formed by once forming a fine particle powder and secondarily pressing it into granules of a certain size. By adjusting the particle size and particle shape as appropriate, it is possible to adjust the ease of taking, the solubility after taking, the immediate effect, and the like.

(Regarding the effect of the first component (corticus)
Tables 1 and 2 are component tables of dry powder of cordyceps.
As shown in Tables 1 and 2, the components of Cordyceps sinensis include protein, lipid, ash, dietary fiber, carotene, polysaccharides, and various mineral components. Moreover, it can be confirmed that arginine is contained in a large amount as a protein component.

冬虫夏草に含まれる有効成分として、ハナサナギン（3,4-diguanidinobytanoyl-DOPA）及びその薬理上許容される塩が知られる（特開2005-239585号公報を参照）。図2は、各種キノコ類における抗酸化活性（フリーラジカル消去活性）の有無と強度比較を示す。

As an active ingredient contained in Cordyceps sinensis, Hanasanagin (3,4-diguanidinobytanoyl-DOPA) and a pharmacologically acceptable salt thereof are known (see JP-A-2005-239585). FIG. 2 shows the presence and absence of antioxidant activity (free radical scavenging activity) and the strength of various mushrooms.

  As shown in this figure, both the extract of hot water and ethanol show excellent antioxidant activity in the fruit body of the moss, and in the cordyceps that grows with the host, other mushrooms. It has excellent antioxidant activity that is several times more than any other kind. This is presumed to be due to a synergistic effect between the hanasanagin and a pharmacologically acceptable salt thereof and the antibody protein produced in association with the invasion of hanasanatake mushroom spores in the host organism. Therefore, in order to obtain an active ingredient of Cordyceps sinensis for this reason, it is preferable to inoculate the mosquito spores in a living state.

  Specific effects of Cordyceps sinensis include analgesia, anti-AIDS, alcohol addiction improvement, anti-allergy, anti-atopic dermatitis, anti-anginal, anti-arrhythmia, as described in, for example, JP-A-2002-272267 Anti-arteriosclerosis, anti-asthma, antibacterial, anti-diabetic, detoxification, anti-inflammatory, anti-hyperlipidemia, DNA mutation suppression, anti-parkinsonian disease, anti-psoriasis, anti-rheumatic, anti-ulcer, brain function protection, cell growth suppression, skin Disease improvement, liver function activation, antihypertensive, immunosuppression, renal function activation, nerve cell protection, intelligence, ophthalmic disease improvement, radiation protection, vascular activation, antiviral, wound medicine, improvement of weak constitution, health, treatment It is effective for.

In addition, it has the activity of promoting the production of tumor necrosis factor from macrophages, and is effective in enhancing immune capacity, preventing anti-cancer, anti-virus, etc., health and treatment.
Furthermore, it has activity to promote production of nitric oxide (NO) from macrophages, and is effective for prevention, health and treatment of cardiotonic, enhancement of myocardial contractility, recovery from fatigue, recovery from physical mental fatigue and the like.
The alcohol extract of Cordyceps sinensis has a function of suppressing the growth of Mycobacterium tuberculosis as an effect on the respiratory system, and its antibacterial properties have been confirmed in athlete's foot fungi, Japanese encephalitis, Bacillus subtilis and the like. In addition, it has a bronchodilator effect that increases acetylcholine, which relaxes bronchoconstriction during asthma, and has also been confirmed to suppress cough and remove sputum. In other words, breathing becomes easier by expelling sputum out of the body.

In addition, acetylcholine is a neurotransmitter, and increasing it activates parasympathetic nerves, so that it can be expected to suppress and treat Alzheimer's disease.
As an effect on the circulatory system, D. mannitol is contained in Cordyceps sinensis, and the coronary artery can be expanded by the component to increase the amount of blood transported to the heart. As a result, blood pressure lowering action, angina pectoris, and prevention of myocardial infarction can be expected. Furthermore, it has been reported to have a hematopoietic effect and an anti-aggregation effect on platelets that form thrombus, and an action to eliminate lipid peroxide has been reported. Taken together, it is understood that Cordyceps is rich in substances that enhance cardiopulmonary function.

Examples of the effect on immunostimulation include polysaccharides such as β-glucan contained in cordyceps. It is understood that the resistance to disease can be increased by the action of the polysaccharide in the lung, liver and kidney.
As described above, Cordyceps is extremely effective as a functional food (edible composition), its raw material, medicine and the like because it has excellent antioxidant activity.

Note that Cordyceps used for the functional food in Embodiment 1 is a natural product obtained by inoculating aseptically cultivated Aspergillus oryzae into aseptically cultivated moths and is extremely reliable without risk of contamination. Therefore, there is no toxicity or very low toxicity, and it is possible to produce an extremely safe functional food by using the component.
Each physiologically active composition derived from Cordyceps can be used alone, but in the present invention, an allo fruit component described later is mixed at an appropriate ratio to obtain a new physiologically active composition, thereby further improving the function. Make up sex food.

(About the effect of the second component (Aronia fruit extract))
On the other hand, aronia fruit is a plant belonging to the family Rosaceae from North America, and is a berry called “Black Choke berry” in North America and “Black Rowan” in Russia. The fruit juice is rich in anthocyanin, an antioxidant polyphenol, and has recently attracted attention as a fruit that has effects such as stress, arteriosclerosis, stool deodorization, antioxidant action, gastric ulcer, immune enhancement and constipation improvement. Collecting.

As shown in FIG. 3, the aronia fruit has an outstanding anthocyanin content even in fruits and the like generally considered to contain anthocyanins.
Table 3 shows the anthocyanin content of each berry. The fruits of the Aronia melanocarpa, a Russian improved varieties listed here, are more fruity than the other 12 berries. It has an anthocyanin content of about 23 times at the maximum and about 14 times at the maximum in the dried fruit state. Here, it is confirmed that it has an anthocyanin content of up to 5 times or more in the fresh fruit state and 4 times in the dry fruit state as compared with a typical blueberry species (V. myrtlillus).

次に、ブルーベリー及びアロニア果実におけるアントシアニンの品種別含有率を表4に示す。

Next, Table 4 shows the anthocyanin content of each cultivar in blueberries and aronia fruits.

表4に示すように、アロニア果実に含まれるアントシアニンは、ブルーベリーとは異なりシアニジン系ポリフェノールが最も多いことが確認されている。当該シアニジン系ポリフェノールは、後述するロドプシン再合成作用の他、排泄物中の胆汁酸量を増加させる作用が強く、これによって体内脂質の排出を促進させ、体脂肪の低減効果を期待できるものである。

As shown in Table 4, it has been confirmed that anthocyanins contained in aronia fruits are the most cyanidin-based polyphenols unlike blueberries. The cyanidin-based polyphenol has a strong effect of increasing the amount of bile acids in the excrement in addition to the rhodopsin re-synthesizing effect described later, thereby promoting the elimination of lipids in the body, and an effect of reducing body fat can be expected. .

  Specific components of the aronia fruit extract are shown in Table 5 below. In Table 5, “Hasukap” refers to Lonicera caerulea L. var. EmphyllocalyxNakai, a deciduous shrub belonging to the genus Honeysuckle family.

表5に示されるように、アロニア果実には無機質、ビタミン、有機酸、糖類、ポリフェノール、アントシアニン、カロチン等が成分として含まれ、さらに食物繊維、タンパク質、脂質、炭水化物、灰分等が豊富に含まれているのが確認される。

As shown in Table 5, aronia fruits contain minerals, vitamins, organic acids, sugars, polyphenols, anthocyanins, carotene, etc., and are rich in dietary fiber, proteins, lipids, carbohydrates, ash, etc. Is confirmed.

  More specifically, cyanidin-based polyphenols contained in large amounts in anthocyanins derived from aronia fruits are mainly composed of cyanidin-3-galactoside and anidine-3-arabinoside. Carotenoids include β-carotene, β-cryptoxanthin and the like. The carotenoid content in aronia fruits is an abundant value comparable to that of pumpkin (eg, about 850 μg per 100 g). Table 5 shows that the amount of polyphenols in the aronia fruit is slightly higher than that of the Hascup fruit and contains about twice that of the blueberry fruit.

  An aronia fruit extract having such a structure has a strong antioxidant effect in the body and is excellent in the action of removing active oxygen that causes cancer and arteriosclerosis. Moreover, when the retina of the eye captures light, it decomposes rhodopsin to produce vitamin A, and a reaction occurs in which rhodopsin is synthesized again, but polyphenols have the effect of promoting the resynthesis of rhodopsin. Taking a large amount of aronia fruit is effective in improving visual acuity, eye fatigue recovery, and dark adaptation. It also has the effect of preventing degradation of glycoproteins and is effective in preventing retinal degeneration and cataracts.

  The anthocyanin intake is preferably 30 mg / day or more for measures against eye diseases and the like. The effect appears about 4 hours after ingestion and disappears after about 24 hours. In addition, if you take 62.5mg / day or more, a medicinal effect appears. Therefore, it is effective not to take a large amount at once, but to continue taking the necessary amount every day (Saburo Ito, Food Industry, August 30, p23 (1997), Osamu Enomoto, Hironari Otani, Kenichi Ogasa, Takahashi Ryo, Food Industry, August 30, p29 (1998)).

Furthermore, it is known that polyphenols have a function of lowering blood pressure by inhibiting the action of angiotensin converting enzyme (ACE) that increases blood pressure in the renin-angiotensin system, which is one of the mechanisms of blood pressure increase in the body. Yes. Therefore, an aronia fruit containing the polyphenol can be expected to have a blood pressure lowering effect.
Further, the aronia fruit extract contains a component derived from a natural product with few side effects and an inhibitor as an inhibitor for cyclooxygenase and inducible nitric oxide synthase. The cyclooxygenase (prostaglandin endoperoxide synthase) is a prostaglandin H2 producing enzyme, and inducible nitric oxide synthase (iNOS) is an enzyme involved in gastric diseases such as cancer development, aging, and gastric ulcer. According to the component, there is an effect of suppressing the action of these enzymes (Japanese Patent Laid-Open No. 2005-213242). Therefore, the effects of anticarcinogenicity, anti-aging, healthy stomach and the like can be expected by taking Aronia fruit extract. This iNOS inhibitory action is also present in the Cordyceps medicinal component, so in the present invention, combining the Cordyceps mexican component and the Aronia fruit component combines the iNOS inhibitory effects of both components, which is superior to taking each component alone. A synergistic effect can be expected.

  In addition, the inhibitor of cyclooxygenase and inducible nitric oxide synthase contained in the aronia fruit extract has an anti-inflammatory action, and is also useful as an immunomodulator, for example, due to its immunoregulatory action. In addition, pharmaceutical effects include blood glucose level adjustment in diabetes, inflammatory eye diseases such as allergic conjunctivitis, iritis, ciliitis, scleritis, glomerulonephritis, pyelonephritis, prostatitis, and bronchitis , Pneumonia, gastroenteritis, rheumatoid arthritis, osteoporosis, colon cancer, duodenal ulcer, Behcet's disease, collagen disease and other autoimmune diseases, urinary tract infection, collagen substrate strengthening, blood platelet coagulation Suppression, capillarity protection (action similar to vitamin P) can be mentioned (Saburo Ito, Food Industry, August 30 issue, p21, p23 (1997), Saburo Ito, Food Industry, August 30 Nikkan, p19, p20 (1998), Tsujimoto Osamu, Otani Hironari, Ogasa Kenichi, Takahashi Ryo, Food Industry, August 30th, p32-34 (1998), Paolo Morazzoni, Food Industry, August 30th, p36-45 (1998)).

In addition, as the content of the aronia fruit extract as an active ingredient in the food and drink for inhibiting cyclooxygenase and inducible nitric oxide synthase of the present invention, the above-mentioned effective amount in the normal daily intake as food and drink The content can be defined to satisfy.
Furthermore, anthocyanins have an effect of linking collagens contained in the skin and strengthening their functions, and also have an effect of improving skin tension and gloss.

On the other hand, β-cryptoxanthin plays a role of a strong barrier when carcinogens and active oxygen attack normal cells, and thus has an effect of suppressing skin cancer and colon cancer in particular.
Furthermore, since it contains abundant dietary fiber, it is effective in relieving constipation and preventing colorectal cancer, and can be expected to have an intestinal regulation effect.
In addition, β-carotene is changed into vitamin A in the body, and the vitamin A has an effect of strengthening the mucous membranes of the retina of the eye, mouth and nose, and keeping the skin, hair, nails and the like normal. Furthermore, it has an antioxidant effect and is effective in preventing lifestyle-related diseases.

In addition, it is known that anthocyanin alone or in combination with mannitol or β-carotene can also exhibit night blindness, capillary fragility improvement, cerebrovascular disorder improvement, gastric ulcer treatment effect, etc. (Ito Saburo, Food Kogyo, August 30 issue, p23 (1997), Osamu Enomoto, Kanari Otani, Kenichi Ogasa, Enshi Takahashi, Food Industry, August 30 issue, p29 (1998)).
<Method for producing cordyceps>
Hereinafter, a method for adjusting cordyceps as a first component of a functional food will be described. The preparation method is roughly classified into the preparation of the spore of moss, the inoculation of cocoons, the cultivation of cordyceps fruit bodies, and the pulverization.

(Adjustment of Solanum spore)
As an example of mycorrhizal fungus, here, Cordyceps saensis (Isaria japonica IM2001 strain), which is collected from the mountains of Tango Peninsula, Kyoto Prefecture and then subcultured in a sterile environment, is used.

The strain Isaria japonica IM2001 is deposited under the deposit number FERMP-18253 at the Patent Microorganism Depositary Center for Biotechnology, Institute of Technology, Ministry of Economy, Trade and Industry (Tsukuba, Ibaraki, 1-1). In order to prevent the occurrence of contamination, it is preferable to use a strain cultured aseptically.
(Process to obtain Cordyceps in a sterile sericulture system)
In the first embodiment, a sterile sericulture rod is mainly used. If the aseptic sericulture system is used to obtain the koji continuously, the stability of the koji of the raw material can be ensured and planned production can be achieved, and the present invention can be realized at a relatively low cost and is extremely useful. .

In addition, by introducing factory automation (FA) into the aseptic sericulture system, it is possible to carry out efficient production, and there is an advantage that cost reduction can be realized by making production and sales distribution more consistent than raw materials.
The process by the aseptic sericulture system is sequentially executed in the following flow a to o.
a. First, in preparation for the rearing tray, lay a dry-proof sheet on the rearing tray to protect the cage from dryness.

b. Next, as a form of the feed, for example, as described in Utility Model Registration No. 2605660, a powdered feed containing dried and pulverized mulberry leaves is kneaded. This is developed on a polypropylene film using a feed molding machine, pressed from above and below the film surface, and formed into a flat plate shape or a wave shape.
c. Place the shaped powdered feed into the rearing tray.

d. Put the rearing tray and sterile feed in the autoclave and steam sterilize.
e. Sterilize eggs (species) with ethyl alcohol in a clean bench and sterilize them.
f. Hatching pupae (2000-3000 per breeding tray) in a sterile petri dish.
g. Raise hatched pupae in a sterile environment.
h. Sweep the sterilized ant pupae onto steam sterilized sterile feed (feed for the first time).

i. Raise the pupae in aseptic environment (1-3 years).
j. After 12 days of sweeping, the feed is changed (4 years old).
k. Reared magnificently (4-5 years old).
l. Maturation and ripening (replacement on tray for making cocoons)
m. Let's manage (make a kite).

n. Remove the cocoon from the cocoon. At this stage, cocoons can be obtained. In addition, since a cocoon is unnecessary in this invention, it can be separately utilized for silk industry etc.
o. Inoculate the bud with spores of the moss, and artificially culture the inoculated cordyceps.
The procedure o will be described in detail. FIG. 4 is a diagram showing the steps from the preparation of the bamboo spore suspension to the completion of the functional food.

Incidentally, the method for artificial culture of Cordyceps sinensis itself is known and is described in detail in Patent Document 2, for example. It is possible to obtain cordyceps in high probability and in a short period of time without worrying about the reproductive contamination of other microorganisms by using the cocoon pupae that have been sterilized as the host of the flower spore.
More specifically, the spore bud spores are dispersed in sterile water to prepare a spore suspension (10 ³ to 10 ⁶ / ml) (S1). 5 to 100 μl, preferably 10 to 30 μl, of this spore suspension is injected into the body of a sterile cage that has been sterilized for all ages using an injector such as a syringe (S2).

Next, the aseptic pods into which the spore suspension has been injected are kept under aseptic conditions at a constant temperature room (0 to 20 ° C.) or at room temperature, and the fruit bodies (corticus) are cultured (S3). Mycelia appear on the surface of the cocoon in about 7 to 8 days, and fruit bodies can be formed in about 1 month.
By the above method, a large number of fruit bodies can be formed in a short period of time within 30 days by optimizing the spore concentration of the spore suspension, the inoculation amount, and the culture temperature after inoculation. According to the experiments by the present inventors, the fruiting body formation rate when the cocoon is used is almost 100%.

As for the inoculation method, in addition to the method of inoculating spore with the above-mentioned syringe, aseptic phlegm is removed under conditions of reduced pressure by means of an aspirator, a vacuum pump, etc. The sputum may be immersed in a spore suspension (10 ³ to 10 ⁶ / ml) and the spores may be forced into the body of a sterile sputum sputum. In this case, any procedure may be adopted as long as the inoculation can be forcedly inoculated into the sterile housing after decompression and release to the atmosphere. Thus, for example, a large number of sterile stubs that can be sufficiently immersed in a container containing a spore suspension (10 ³ to 10 ⁶ / ml) are placed in a larger container under reduced pressure conditions of 200 mmHg to 760 mmHg. You may employ | adopt the method of hold | maintaining for ~ 60 minutes and forcing infiltration in a sterile housing. It is presumed that the following phenomenon has occurred by reducing the pressure. That is, the reduced pressure makes it easier for the spore suspension to evaporate, and the air in the bag expands and diffuses, and the body fluid volatilizes due to the reduced pressure, creating a void in the bag. Thereafter, when the soot is immersed in the spore suspension at the time of opening to the atmosphere, the air in the soot that has been expanded by the atmospheric pressure contracts, and the spore suspension enters all corners of the soot. Or the spore suspension permeates into the space after the body fluid has volatilized. By the reduced pressure infiltration inoculation method, spores can be inoculated into the cage more uniformly than in the direct injection method. Furthermore, according to the vacuum infiltration inoculation method, it is possible to inoculate a large number of pupae at once. By continuing further aseptic culture after inoculation, almost 100% fruiting bodies can be formed within about one month or more in the same manner as when injected into the body with a syringe.

  In the first embodiment, the production method for inoculating a pupa with a scallop spore was illustrated, but the present invention is not limited to this, and the spore may be inoculated with a spore from a pupa. In this case, since the weight of the pupae is about 1.5 to 2.0 g, and the weight of the larvae is about 7 g, it seems that the production efficiency is higher when the larvae are used. However, at the larval stage, the inoculated spore of spores is excreted and the remaining amount in the body may be reduced.

In addition, even when inoculated into the larvae or pupae after death, it is possible to obtain cordyceps as the host, but in this case, the host does not produce antibody proteins associated with the invasion of the moss. Therefore, it is preferable to inoculate a living body to obtain an active ingredient.
In addition, according to the method of the present invention, there is an advantage that the cocoon is taken out and the cocoon is taken out and used separately for the production of silk thread. Therefore, it is possible to implement the present invention together with the conventional sericulture industry, and there is a merit that the work efficiency is less wasteful and can be implemented extremely rationally and efficiently.

  Next, extraction of the active ingredient from Cordyceps sinensis that formed fruit bodies is performed using ethanol, a water-ethanol mixture or water as a solvent. Preferably, extraction is performed by slowly stirring at room temperature or under warming conditions using a water-ethanol mixture of 10 to 90% ethanol as a solvent (S4). The amount of ethanol added and the extraction time are not particularly limited.

The extract is filtered and an ethanol extract is obtained. In this ethanol extract, fat and oil components and water-soluble components are extracted. In addition, when extracting only fats and oils components, it uses as organic solvents, such as butanol, ethyl acetate, butyl acetate, isobutyl acetate, n-hexane or chloroform, xylene, acetone.
However, since it is used as a functional food or the like, it is necessary to completely remove this organic solvent in a subsequent process. The extracted fat and oil component can be used as a functional food as in the case of the water-soluble component described later. In the above organic solvent, it is preferable to extract under pressure and heating conditions of 20 to 80 ° C.

Next, extraction is performed by adding water to the filtration residue obtained by ethanol extraction and heating at 50 to 140 ° C. with gentle stirring, or pressurizing and heating as necessary. Preferably, hot water extraction is performed under a pressure heating condition of 80 to 130 ° C. The extract is filtered and separated to obtain a first hot water extract.
Furthermore, hot water extraction is performed under the same pressure and heating conditions as described above while adding water to the filtration residue and gently stirring, and then filtered to obtain a second hot water extract.

The first hot water extract and the second hot water extract can be used as active ingredients of the composition of the present invention after autoclaving at 121 ° C. for 15 minutes, respectively. The amount of water used for extraction is not particularly limited. Moreover, what was extracted without removing an extraction residue if necessary can be used as an active ingredient containing composition as it is.
Further, Cordyceps is dried and then finely pulverized to obtain a powder (S5). This is the end of the manufacture of cordyceps. The powder is ready for use as a physiologically active composition.

In the present invention, the extraction step is not essential, and powders may be obtained by directly drying and crushing cordyceps (S6). In other words, any of the steps S4 and S5 and the step S6 can be selected.
(Adjustment of Aronia fruit extract components)
Next, the Aronia fruit extract is adjusted.

Add 1 kg of water to 1 kg of harvested aronia fruits, place in a Waring blender, homogenize, and extract with stirring at about 30 ° C for 2 hours.
After extraction, the solution was centrifuged and filtered under reduced pressure using filter paper. The filtrate was concentrated under reduced pressure using a rotary evaporator to remove water. The final extract is obtained and the powder is obtained by lyophilization. For food and drink applications, the powder can be used as it is as an aronia fruit extract.

  In addition, the aronia fruit component is not limited to the method of refining by the above method, but may be obtained by directly pulverizing and drying the fruit, or may be dried by adding sugar and fruit juice separately. When aronia fruits are used as an extract, squeezed cocoons of aronia fruits are generated as a by-product, and the squeezed potatoes also contain anthocyanins. If the fruit is ground directly, such anthocyanins can be used without waste.

The prepared powder of aronia fruit extract and the prepared powder of cordyceps are mixed (S7).
The aronia fruit extract itself has a low sugar content and a strong astringency compared to other berries, so that the taste can be adjusted by appropriately adding sugar.
In the first embodiment, thereafter, a sweetener such as sugar is appropriately added as a taste adjustment (S8).

Through the above steps, the functional food 1A of the present invention is produced (S9). Thereafter, the functional food 1A is packaged in a necessary form. In order to keep the antioxidant activity of the functional food of the present invention satisfactorily, it is preferable to fill the package with an inert gas and to configure the package with a light shielding material.
<Confirmation experiment on the effectiveness of Cordyceps sinensis>
Recently, it has been reported that antineoplastic polysaccharides (polysaccharides) derived from mushrooms promote macrophage tumor necrosis factor (TNF-α) secretion in vivo. Is also expected to have this function. TNF-α, an endogenous factor that exhibits tumor-selective toxicity, is an important defense factor against tumor cells, and a polysaccharide called Lentinan activates NK cells, including TNF-α secretion. It is known to become an antineoplastic agent that promotes macrophage function.

On the other hand, NO, a free radical gas, is produced by NO synthase (NOS) induced by polysaccharides, and is involved in various functions such as information transmission, immune response, inflammation, and platelet aggregation suppression in vivo. . Thus, TNF-α and NO are considered to act as antitumor cell activities and factors that exhibit various physiological activities in the immune system.
Therefore, the inventors searched for an immune function-modulating polysaccharide that promotes the production of TNF-α and NO from the culture in order to confirm that Isaria japonika actually has these functions (Kinugasa Fiber Research Institute). (2003) No. 7, 11-17).

  Specifically, the fruit body of the flower moth using the aseptically cultivated sputum at the Institute for Aseptic Culture (Kyoto) was used. This was ground in liquid nitrogen using a Waring blender and then freeze-dried. The dried product was extracted three times with an aqueous ethanol solution at 80 ° C. for 3 hours, and the resulting extract was filtered. This was degreased with 1.5 volumes of chloroform for 12 hours, and the aqueous layer was lyophilized to obtain the first fraction.

Furthermore, the residue of the ethanol treatment liquid was extracted with hot water for 6 hours and filtered. Ethanol was added thereto, and the mixture was centrifuged at 8,000 G for 30 minutes. The precipitate was dissolved in water and dialyzed against pure with a cellulose tube. The dialyzed solution was concentrated under reduced pressure and lyophilized to obtain a second fraction. Furthermore, the centrifugal supernatant was concentrated under reduced pressure and lyophilized to obtain a third fraction.
Each fraction was first subjected to bioassay using actinomycin D-treated mouse fibroblast L-929 in order to measure TNF-α activity.

On the other hand, NO synthase was measured by assaying NO2- of the culture supernatant, which is a stable reaction product of oxygen molecules, by the Stuehr-Nathan method.
As a result, the presence of a polysaccharide having a molecular weight of about 8,000 was confirmed in all of the obtained fractions. Although this molecular weight is small compared to the molecular weight of antitumor polysaccharides known so far (about 400,000 or less), very high TNF-α activity was confirmed. Therefore, it can be said that antitumor polysaccharide is effectively produced also in Cordyceps.

<Experiment on effectiveness of aronia fruit components>
As part of the search for a new therapeutic agent for rat endotoxin-induced uveitis (EIU) using aronia fruit extract, the therapeutic effect of ACE in EIU has been studied ("Efficacy of aronia against eye disease") , Kazuhiro Ogami, 2005 Second Aronia Study Group / Lecture).

  As a specific study method, Lewis rats were administered with ACE of 1, 10, 100 mg / 0.1 ml / rat in the tail vein immediately after LPS administration. After 24 hours, the anterior aqueous humor was collected, and the number of inflammatory cells, protein concentration, NO concentration, PGE2 concentration and TNF-α concentration in the aqueous humor were measured. In addition, 10mg prednisolone was performed on the same schedule. Furthermore, the expression of NO synthase (iNOS) and PG synthase was examined using RAW264.7 cells using the Western Blotting method.

As a result, compared with the LPS group, ACE significantly suppressed the number of inflammatory cells, protein concentration, NO concentration, PGE2 concentration and TNF-α concentration in the anterior aqueous humor induced by LPS. The anti-inflammatory effect of 100 mg ACE group was almost the same as 10 mg prednisolone.
ACE was also found to significantly suppress LPS-induced iNOS and COX-2 protein expression in a concentration-related manner.

Thus, in rat endotoxin-induced uveitis, ACE showed a concentration-related inhibitory effect, and in particular, the inhibitory effect of 100 mg ACE was almost the same as that of 10 mg prednisolone. This suggests that ACE has potential as an anti-inflammatory drug for ocular inflammation.
<Other matters>
(About manufacturing method)
In the first embodiment, the first component and the second component are individually exemplified for the production method (milling), but the present invention is not limited to this, and the first and second components are After mixing, milling (grinding) treatment can be performed. In this case, a good stirring effect of the first and second components can be expected, and mixing between them at the same time may cause an interaction between the two components and may form an active ingredient. .

(About the form of the functional food of the present invention)
In the first embodiment, the functional food 1A, 1B is exemplified as a powder or granule, but the functional food of the present invention may be in other food forms. The form examples are summarized as powders (powder, granules, fine granules), pills, tablets (tablets, naked tablets, dragees, glued tablets, laminated tablets, dry tablets, effective tablets, release tablets, film coatings) Tablets, enteric-coated tablets, effervescent tablets, fast-dissolving preparations), capsules (hard capsules, soft capsules), troches, liquids for internal use (liquids for internal use, liquids for internal use, shaking mixtures, suspensions, Emulsions, syrups, elixirs, roasting agents, limonades, saturants, tinctures, fluid extracts, spirits), extracts, external solutions (external solutions, injections, gargles, poultices, inhalants, coatings) Agent, bath agent), ointment (greasy ointment, emulsion ointment, water-soluble ointment, mud, rubbing agent, lotion agent), plaster, spray (sprayer type, aerosol type), cataplasm, etc. Can be mentioned.

  Furthermore, various additives can be combined with the functional food of the present invention as necessary. Examples of such additives include sweeteners, acidulants, vitamins, starches, lactose, sucrose, erythritol, xylitol, mannitol and other sweeteners, additives such as trehalose, carboxymethylcellulose, corn starch, inorganic salts, or binders, Disintegrating agents, surfactants, lubricants, fluidity promoters, flavoring agents, coloring agents, fragrances, stabilizers, preservatives, pH adjusting agents such as citric acid and tartaric acid, and the like can be added.

  In addition, the functional food of the present invention can be used in combination with other functional food materials and the like. Further, it is used as a seasoning for dishes such as soft drinks, confectionery (jelly, gummy, etc.), frozen confectionery, dairy products, alcoholic beverages, and meats. Etc. (jam etc.) can also be used.

  The present invention can be widely used as a seasoning or the like in addition to functional foods (so-called health foods, health drinks, and insurance functional foods) for humans and animals, or nutritional supplements.

FIG. 2 is a schematic particle structure diagram of a functional food according to Embodiment 1 of the present invention. It is a graph which shows the free radical scavenging activity of various mushrooms and cordyceps. It is a graph which shows content of polyphenol, such as various fruits. It is a figure which shows the typical manufacture flow of the functional food of this invention.

Explanation of symbols

1A, 1B Functional food
10A dry particles of the first component
10B granules
11A Dry particles of the second component
12A Taste control additive particles

Claims (6)

A functional food comprising a composition obtained by mixing a first component obtained from Cordyceps sinensis including Isana japonica IM2001 (FERMP-18253) and its host and a second component obtained from aronia fruit. 2. The functional food according to claim 1, wherein the host in the cordyceps is a sterilized pupae. 3. The functional food according to claim 1, wherein the composition is powdery or granular. The functional food according to any one of claims 1 to 3, wherein the first component is a product obtained by extracting the dried caterpillar from the organic solvent under high temperature conditions. The first component of Cordyceps sinensis obtained by inoculating the spore of the fungus, Isaria japonica IM2001 (FERMP-18253),
A method for producing a functional food, comprising a mixing step of mixing a second component obtained from an aronia fruit. 6. The method for producing a functional food according to claim 5, wherein in the mixing step, the powdered first component and the second component are mixed.

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