RU2572215C1 - Therapeutic and energetic agent for animals - Google Patents

Abstract

FIELD: veterinary medicine.

SUBSTANCE: agent comprises natural honey, bee pollen hydrolyzate, hydrolyzate of muscle tissue of minks, theotropine, distilled water with the following ratio of components, g/100 ml: natural honey 25.0±2.5; bee pollen hydrolyzate 2.5±0.25; hydrolyzate of muscle tissue of minks 12.5±1.25; theotropine 0.1±0.0; distilled water - the rest.

EFFECT: agent is highly effective to increase the activity of metabolic processes and immunity in animals.

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Description

Bracing agent for animals.

The invention relates to the field of veterinary medicine, namely to general restorative agents based on beekeeping products and animal proteins to increase the activity of metabolic processes and immunity in animals.

In animals with various diseases, increased physical exertion, increased productivity, there is a lack of energy, metabolic disorders, and suppression of the immune system.

At the same time, the vitamin and nutritional value of the diet of animals is very important. It is almost impossible to achieve an optimally balanced diet that ensures the replenishment of energy costs, amino acids, vitamins, macro- and microelements, without the introduction of additional biologically active additives. Therefore, a constant search is underway for additional funds that increase the potential reserves of the body, optimizing and accelerating the recovery process. General strengthening remedies based on bee products, which delay the onset of the signaling role of fatigue by expanding the biochemical and functional reserves of the body, are widely used in this area.

Means based on beekeeping products have a high energy value, as they contain simple sugars that can be directly absorbed into the blood. This is very important for animals who urgently need to replenish the energy expended.

In addition to energy value, beekeeping products are a source of di- and monosaccharides, vitamins, micro-, macrocells. In addition, in order to replenish amino acids in the diet, the inclusion of a protein hydrolyzate is necessary.

When studying various sources of literature, we did not find any registered drugs that are allowed for use by animals and have this effect, so there is a need to develop such a tool.

Known means for apitherapy, which has a tonic and adaptogenic effect, which includes dry Eleutherococcus extract, dry licorice root extract, dry rosehip extract, Estifanum, Selimar, propolis tincture, natural honey [1].

The disadvantage of this tool is that it contains plant adaptogens that are classified as stimulants of a doping nature. In addition, with prolonged use, cumulation and habituation of the body are possible.

In large doses, they are able to provide a stimulating effect with the expenditure of energy.

Known prophylactic and restorative agent of a wide spectrum of action, containing honey, ascorbic acid, spirulina algae powder, calcium stearate or stearic acid in the following ratio of components, mass. %: honey 22-28; ascorbic acid 8-12; calcium stearate or stearic acid 0.8-1.2; spirulina algae powder - the rest [2].

The disadvantage of this tool is that it does not contain easily digestible protein. In addition, this tool has a rather high honey content, while other introduced prescription components cannot provide a sufficiently high level of biological value.

A well-known bioactive preparation containing natural honey, cedar oil, sprouted wheat grain, with the following ratio of components: cedar oil 5.0-10.0%, sprouted wheat grain 65.0-70.0%, natural honey - the rest . [3]. This invention was taken as a prototype.

The disadvantage of this drug is that, in addition to honey, it contains only plant components (cedar oil, wheat grain) that contain a small amount of easily digestible protein and, accordingly, cannot make up for the body's need for it.

The objective of the invention is the construction of a relatively inexpensive effective means based on honey and hydrolysates, which ensures the replenishment of animal energy costs, amino acids, vitamins, macro- and microelements, thereby increasing the potential reserves of the body and speeding up the recovery process.

This object is achieved in that the proposed restorative agent for animals contains natural honey, hydrolysates of pollen and muscle tissue of minks, theotropin and distilled water in the following ratio of components, g / 100 ml:

An essential feature of the proposed tool is its qualitative and quantitative composition, which allows to obtain a highly effective product with energy and regenerative effect, without causing side effects.

As part of the proposed product, bee products (honey, pollen hydrolyzate) are supplemented with a mink muscle hydrolyzate in the declared dose, which is a source of readily available protein and essential amino acids, which provides replenishment of the complex of substances necessary for the body (carbohydrates, proteins, amino acids, vitamins, macro- and trace elements), thereby contributing to a more rapid recovery of the body after a disease, high physical exertion, increasing the reserve capacity of the body.

Natural honey contains: minerals, sugars, enzymes, organic and inorganic acids, plant hormones (phytohormones) and hormones supplied with the secretion of the mandibular glands of honey bees, vitamins (vitamin C, E, group B, H, PP, etc. .), biologically active phenolic compounds [4]. All these components are involved in many biochemical processes of the body.

The pollen hydrolyzate contains more than 50 biologically active substances that have a positive effect on physical, nervous, mental overfatigue, disorders in growth and development, and a number of diseases [5].

The hydrolyzate of mink muscle tissue is characterized by a high degree of digestibility of amino acids. It supplies the body with such amino acids as tryptophan, methionine and cysteine, improves binding and accelerates the absorption of calcium and zinc, promotes the formation of peptides with antibacterial and immunoregulatory properties, and stimulates the growth of bifidoflora in the intestine [6].

Theotropin is a preservative and is introduced into the product to increase the shelf life.

Thus, these components in the stated doses have a complex effect on the body, facilitate the entry into the body of biologically active substances in an easily accessible form, which is important in animal husbandry due to the need to recover from illness, high physical exertion, and overuse.

The inventive tool is a thick viscous liquid of yellow-brown or brown color, the presence of sediment obtained by dissolving honey in water, hydrolyzed pollen and muscle tissue of minks, theotropin is allowed.

The following components are used to prepare the claimed preparation: natural honey according to GOST 19792-2001; pollen hydrolyzate according to GOST 28887-90, which is a thick, heterogeneous brown liquid containing flower pollen; the mink muscle tissue hydrolyzate according to TU 49 979-85 is a white powder with a yellowish tint, free-flowing powder obtained by drying the protein hydrolyzate concentrate, theotropin is a white powder.

To prepare the product, the following is introduced into a 100 ml flask: 25.0 ± 2.5 g of natural honey, 2.5 ± 0.25 g of pollen hydrolyzate, 12.5 ± 1.25 g of mink muscle tissue hydrolyzate, 0.1 ± 0 , 01 g of theotropin, add a small amount of distilled water and stir until the components are completely dissolved, bring the volume to 100 ml.

The product is packaged in bottles of 100 or 200 ml. Before use, the drug is diluted in water to 500-1000 ml and fed to the animal in a therapeutic dose.

Monitoring the quantitative content of components (natural honey, hydrolyzed pollen and muscle tissue of minks) in the proposed tool is carried out in accordance with GOST and TU for these substances [4; 5; 6].

Biological studies on laboratory animals (rats, white mice, guinea pigs, rabbits) of the proposed product showed that with a single injection of the product into the stomach to white mice and rats at a dose of 1000, 5000 and 10000 mg / kg it was not toxic.

In chronic experiments on animals (white mice and rats), there were no symptoms of the toxic effect of the drug when administered within 1 month.

The allergenic properties of this agent were investigated in guinea pigs. When applying the product on the skin of guinea pigs daily for 15 days, no allergic reaction was observed.

Experiments on rabbits on the presence of a local irritant in the proposed product showed that with a single application of the product on the skin of rabbits 4 hours and 14 days after application of signs of irritation was not observed.

In addition, the proposed tool is stable throughout the entire storage time (tests were carried out for 36 months), no modification of the tool was observed.

Example 1. Determining the effect of funds on the general condition of the body of clinically healthy calves

The experiment was carried out on 20 clinically healthy calves. For this, the calves were asked the claimed agent in 1 dose (50 ml), diluting it with water to 500 ml, 1 time per day for 3-5 days. During the experiment, they monitored the general condition of the calves. To determine the effect of the drug on metabolic processes in the calf’s body, hematological and biochemical parameters of blood were monitored before the start of the dosing and after the end of the dosing.

As a result of the experiment, it was determined that during a clinical examination of the experimental calves during the experiment, all the calves were clinically healthy. Eating food and water consumption are satisfactory, body temperature, pulse, respiration within the physiological norm, mucous membranes are smooth, shiny, pink, active movements, deviations from individual organs and systems were not observed.

Hematological blood tests showed that the claimed tool does not adversely affect the morphological composition of the blood of calves. When using the drug there was a significant increase in the blood of erythrocyte content by 12.8%, hemoglobin content by 13.5%, hematocrit by 13.2%. The remaining hematological parameters were not changed.

A study of biochemical blood parameters showed that this tool does not adversely affect the biochemical blood parameters of calves. After the introduction of calves into the diet, biochemical parameters did not go beyond the physiological norm. There was an increase within the physiological norm of the content of serum calcium, iron, glucose and protein. At the same time, the calcium content increased by 60.7%, the iron content increased by 21.8%, the glucose content increased by 12.2%, the protein content increased by 13.3%. The remaining biochemical parameters were not significantly changed.

Thus, the claimed tool not only does not adversely affect the general condition and metabolic processes in the body of calves, but also improves the hematological and biochemical parameters of young growing animals.

Example 2. The effect of a bracing agent on the metabolic processes of calves who have had pneumoenteritis

The experiment was carried out on 20 calves. For testing, two groups of calves were formed (experimental and control). The experimental group included 10 calves who were ill with pneumoenteritis, who were fed the claimed remedy, and the control group consisted of 10 clinically healthy calves.

The calves of the experimental group 1 time per day, before feeding, were asked the claimed tool, diluted in 500 ml of water, at a dose of 50 ml per head, for 3-5 days. Feeding, maintenance and volume of physical activity were the same in the experimental and control groups. During the experiment, we observed the general condition of the calves of both groups. To determine the effect of the drug on the metabolism in the body of calves, hematological and biochemical parameters of blood were monitored before the start of giving and after the end of giving.

The results of hematological studies of calves of the experimental and control groups before and after the experiment are given in table. one.

When comparing the results of hematological studies of calves of the experimental and control groups before and after the experiment, it can be seen that in the calves of the experimental group, the hematological parameters improved significantly.

The results of a biochemical study of blood serum of calves of the experimental and control groups before and after the experiment are given in table. 2-3.

The biochemical blood parameters of the calves of the experimental group improved after the end of the experiment.

Example 3. Determining the effectiveness of the tool on calves with pneumoenteritis

To study the effectiveness of a general strengthening agent, studies were carried out in two livestock farms (Farm No. 1 and Farm No. 2). Farms are typical of livestock production using the technology used to obtain and grow young animals. The sanitary condition of livestock buildings is satisfactory. Farms are unsuccessful for viral and bacterial enteritis of calves, the incidence of which was 80.4-92.2%. The therapeutic regimen for these diseases included a 6-8 hour fasting diet with a decoction of medicinal herbs, antimicrobial (enrofloxacin and amoxicycline), rehydration (infusion of isotonic sodium chloride solution), antitoxic (intravenous infusion of 40% glucose solution) therapy.

To study the therapeutic efficacy of the drug from diseased calves, they were formed taking into account the principle of conditional analogues in 2 groups at the age of 20 days, 20-25 goals each.

All experimental animals were treated according to the following scheme. When the first signs of the disease appeared, calves were stopped drinking colostrum and replaced with isotonic fluids. Then, over the course of two days, they gradually switched to the usual feeding regimen.

The calves of the 1st experimental group for therapeutic purposes used the claimed restorative agent, which was added to soluble saline or boiled water at a dose of 50 ml per 500 ml of liquid.

Animals of the 2 groups served as a control and were treated according to the above scheme adopted on the farm.

All experimental animals were monitored daily. Conditionally, recovery was taken as an improvement in the general condition of a sick animal and the disappearance of diarrhea.

The determination of the therapeutic efficacy of the drug was carried out by mortality, duration and severity of the disease. The research results are presented in table. four

In animals of the experimental groups, the disease proceeded mainly in a mild form. The main symptoms were mild depression, decreased appetite, increased intestinal motility, frequent bowel movements with the release of liquefied yellow feces, sometimes with mucus. The disappearance of clinical signs of gastrointestinal disturbances occurred mainly on the 2nd – 3rd day of treatment. 1-2 animals fell.

The use of fortifying agents in households No. 1 and No. 2 allowed to reduce mortality by 2-4 times and reduce treatment time by 2 times.

Thus, the introduction into the diet of calves of a general restorative agent based on beekeeping products and muscle protein hydrolysates contributes to an increase in animal activity, an increase in blood levels of red blood cells, hemoglobin, as well as total protein and a decrease to normal. These changes in the blood composition of calves indicate the normalization of a number of biochemical processes in the body, an increase in the amount of oxygen consumed, and in general on the beneficial effect of the drug on the body of calves.

Information sources

1. Patent RU 2098108, IPC ⁶ A61K 35/64, A61K 35/64, A61K 35/78; 12/26/1995.

2. Patent RU 2145228, IPC ⁷ A61K 35/64, A61K 35/80; 03/23/1999.

3. Patent RU 2126217, IPC ⁶ A23L 1/30, A23L 1/08, A61K 35/78, A61K 35/64; 08/19/1997 (prototype).

4. Honey natural. GOST 19792 - 2001.

5. Flower pollen. GOST 28887 - 90.

6. A hydrolyzate of the mink muscle tissue. TU 49 979-85.

Claims (1)

A general strengthening agent for animals containing natural honey, characterized in that it additionally contains a bee pollen hydrolyzate, a mink muscle hydrolyzate, theotropin, distilled water in the following ratio of components, g / 100 ml: natural honey 25.0 ± 2.5; bee pollen hydrolyzate 2.5 ± 0.25; hydrolyzed muscle tissue of minks 12.5 ± 1.25; theotropin 0.1 ± 0.0; distilled water - the rest.