RU2561689C1 - Agent for treating diseases of liver in cattle and pigs, increasing their safety and productivity - Google Patents

Abstract

FIELD: veterinary medicine.

SUBSTANCE: agent comprises di-acetophenonylselenide - 0.05-0.1; beta-carotene 0.09-0.18 and essential phospholipids - 5.5-6.0, dissolved in vegetable oil (wt %).

EFFECT: agent enables to reduce the time of treatment in liver pathology in cattle and pigs, as well as to increase their safety and productivity.

4 tbl, 2 ex

Description

The present invention relates to veterinary medicine, namely to medicines intended for the treatment of liver diseases in farm animals, as well as increasing their safety and productivity.

Animal liver disease is one of the most common pathologies. At present, the problem of normalizing the metabolism in animals, as well as therapy, and especially the restoration of the normal structure and function of the liver, is far from being resolved; therefore, it seems justified to search for new, more effective means of targeted action in hepatopathies, which at the same time have the ability to increase the productivity and safety of animals .

Known drugs used to provide medical care for hepatopathies, such as: methionine, hemodesis, tocopherol, vitamin U, urotropin and choleretic drugs [1]. However, the effect of these drugs is aimed at eliminating toxins from the body, bile - from the patient’s liver, relieving pain, but they do not restore the damaged structure and function of the liver.

Known drug Hepatovet [2], which is a suspension for oral administration, which includes phospholipids, flavonoids and amino acids - methionine and ornithine. Indications for use of the drug are developed only for dogs and cats, while its shelf life after opening the bottle is no more than 21 days.

Known drug Carcel, including diacetophenonylselinide, beta-carotene and vegetable oil - used for the prevention and treatment of postpartum complications in cattle [3].

The closest in technical essence is a tool to increase the safety and productivity of animals [4], taken as the closest analogue, including in wt. %: diacetophenonylselinide - 0.18-0.24; beta-carotene 0.1-0.2 and lycopene 0.1-0.2. However, the closest analogue is used only to increase the safety and productivity of animals, without showing pronounced therapeutic effectiveness in liver diseases in cows and pigs.

Thus, the above funds either do not affect the liver at all, or cannot fully protect or restore the impaired functions of the membranes, mitochondria, and other structures of hepatocytes, since any of them affects only certain links of pathogenesis in hepatopathology. Known funds do not allow for a complex effect, showing therapeutic efficacy in liver diseases in farm animals and lead to a simultaneous increase in productivity, preservation, and normalization of metabolic processes in their body.

The technical result of the use of the proposed tool, including the organic form of selenium, carotenoids and phospholipids, is an effective combination of hepatoprotective and antioxidant factors with a simultaneous increase in the safety and productivity of farm animals.

Objective: expanding the arsenal of funds for the treatment of liver diseases in animals, as well as increasing their safety and productivity.

The advantage of the proposed product compared to the above drugs is as follows:

1) the product is made in the form of a solution both for oral use, and in the form of a solution for injection, while it has low toxicity, high efficiency, and also a long shelf life;

2) the injection form provides a higher degree of release of active substances and the speed of the onset of the effect, while all components in this dosage form are stored in their native state;

3) it is recommended for hepatopathies in animals (cattle, pigs) with a parallel effect of increasing safety and productivity (milk yield, weight gain);

4) the advantage of the claimed funds is the mutual reinforcement of the beneficial pharmacological properties of each incoming ingredient when exposed to the whole organism of the animal as a corrective system;

5) the availability of the drug due to its lower cost and domestic production.

This object is achieved in that the agent for treating liver diseases in cattle and pigs, increasing their safety and productivity, includes the target components diacetophenonylselinide (DAFS-25), beta-carotene, vegetable oil and essential phospholipids with the following components, wt. %:

DAFS-25 0.05-0.1 beta carotene 0.09-0.18 phospholipids 5.5-6.0 vegetable oil rest

The claimed tool differs from the closest analogue in the inclusion of substances that deliberately affect liver cells - essential phospholipids, as well as a reasonable concentration of diacetophenonylselinide and beta-carotene (since the applicants found good compatibility and synergism of phospholipids, carotene and diacetophenonylselinide, which allowed to reduce the concentration of A and trace element selenium, while increasing the effectiveness of the results of use). Therefore, the factors that determine the effect of the obtained product with the desired properties include not only the content of chemical components included in its composition, but also their claimed volume in wt. %

If the content of diacetophenonylselenide in the composition of the product is less than 0.05%, then this does not provide the claimed effectiveness of the drug. If the content of diacetophenonyl selenide in the composition of the product is more than 0.1%, then this will increase its cost without enhancing the therapeutic effect. Therefore, the optimal dosage of selenium (DAFS-25) in the product is the indicated concentration of 0.05-0.1 on average 0.075%.

If the content of beta-carotene in the composition of the product is less than 0.09%, then this does not provide the claimed effectiveness of the drug. If the content of provitamin A in the composition of the product is more than 0.18%, then this will increase the cost of the drug without increasing the therapeutic effect. Therefore, the optimal dosage of beta-carotene in the product is the indicated concentration of 0.09-0.18 on average 0.14%.

If the content of phospholipids in the composition of the product is less than 5.5%, then this does not provide the declared effectiveness of the drug, if their concentration in the composition of the product is more than 6.0%, then this will increase the cost of the drug without increasing the therapeutic effect. Therefore, the optimal dosage of phospholipids in the product is the indicated concentration of 5.5-6 on average 5.75%.

As a result of using the proposed tool, the expected economic effect will be: 5841 rubles per cow, 1720 rubles per pig.

These differences and the resulting technical result allow us to conclude that the claimed technical solution meets the criteria of "novelty" and "inventive step".

The production name of the new product is the word “Hepavitol”, formed from the abbreviation of the words hepar (liver) - “hepa”, vita (life) - “vit”, oleum (oil) - “ol”.

As follows from the following explanations, “Hepavitol” has a new set of features. It provides not only the sum of the effects of each component separately - the substances included in the composition of the drug, taken in the optimal ratio, show a synergy of the mutual influence of the components and have a complex effect on the liver, as well as on all systems, organs and tissues of the body, which leads to increased productivity (weight gain and milk yield) and the safety of animals.

The protective effect of many hepatoprotectors is based on the inhibition of lipid peroxidation processes, which are considered as one of the leading pathogenetic mechanisms of the development of liver lesions. Therefore, the use of antioxidants, such as beta-carotene and selenium, is reasonable.

The antioxidant system of the body includes the enzyme system (selenium-dependent glutathione peroxidase, catalase, etc.), and the non-enzymatic link is represented by such biooxidants as carotenoids, vitamin E, etc., therefore, the proposed combination of substances acts at all levels of protection, ensuring the normalization of lipid peroxidation .

In addition to the antioxidant properties, carotenoids have other valuable properties that have a positive effect on the animal organism: anticarcinogenic, detoxifying and immunostimulating activity, which allows to increase the productive qualities of farm animals - reducing mortality and increasing the quantity and quality of the products obtained.

Selenium provides the activity of redox enzymes and vitamins, affects the synthesis of thyroid hormones, increases the immunological resistance of the body, the synthesis of protein cytochrome P-450, as well as the content of amino acids in plasma and liver.

Essential phospholipids serve as a building material in cell membranes. Their main functions are to maintain normal fluidity and repair of cell membranes, antioxidant action, protect mitochondrial and microsomal enzymes from damage, slow down collagen synthesis and increase collagenase activity. The membrane stabilizing and hepatoprotective effect of phospholipids is achieved by directly incorporating molecules into the structure of damaged liver cells, replacing defects, and restoring the barrier function of the lipid bilayer of membranes. Exogenous phospholipids promote the activation of phospholipid-dependent enzymes located in the membrane. This, in turn, has a supporting effect on metabolic processes in the liver cells, contributes to an increase in its detoxification and excretory potential.

Thus, the proposed combination of components in the claimed agent selectively affects hepatocytes (helps to intensify the processes of cell metabolism and metabolism, redox processes, cell regeneration, lipid metabolism), which positively affects the course of treatment of liver diseases in animals with the realization of the increase effect their safety and productivity.

The industrial effectiveness of the use of agents for treating liver diseases in farm animals, increasing their safety and productivity is illustrated by approbation.

Scientific and industrial experience was carried out on cows of black-motley breed at the age of 4-6 years. Hepatosis was diagnosed using clinical and laboratory tests.

Simulating the experiment, we selected animals that did not use an active exercise during the entire stall period. The diet was represented by a highly concentrated type of feeding with a violation of the sugar-protein ratio and an insufficient content of vitamins and minerals. Cows were above average weight, body temperature, heart rate and respiratory movements within normal limits. Clinical examination revealed peeling of the epidermis in all animals, in 50% - extensive alopecia, in 20% - dystonia of the pancreas with a violation of rhythm and strength of ruminations. In 40% of cows, the percussion borders of the liver are enlarged, in 10% it is painful on palpation. In most cows examined, the last caudal vertebrae are softened. Yellowness of the skin and mucous membranes was absent.

Blood samples for laboratory studies were taken at the beginning and at the end of the experiment. With the background biochemical, it was found that impaired liver function was confirmed by the following indicators: hypoproteinemia (a decrease in the level of total protein to 61.3 g / l against 79-89 g / l normal); disturbances in proteinograms were characterized by a decrease in albumin and an increase in β- and γ-globulin fractions; low urea level (2.4 mM / L versus 3.3 mM / L normal); positive thymol test; increased activity of aminotransferases; the concentration of vitamins (carotene, tocopherol) beyond the lower limit of normal.

Cows were divided into 2 groups of 30 animals each: the first - positive control (treatment according to the scheme adopted on the farm: adsorbents, urotropin, choleretic drugs); the second - experience (therapy according to the scheme: “Hepavitol” agent, subcutaneously, 15 ml every 2 days for 30 days, according to indications, the course of treatment can be repeated).

The effectiveness of therapy was evaluated by the clinical condition of the cows, forced slaughter, data from a biochemical blood test.

In the group of positive control, five animals were forcedly slaughtered, in the group using "Hepavitol" there was no death (100% safety compared to 83.3%).

At the end of the experiment, analyzing the data of biochemical studies of blood of cows after treatment, it was found that the effect of Hepavitol on protein metabolism was characterized by a significant increase in the level of total protein by 16.2% in comparison with the positive control. This process was accompanied by stabilization of its fractional composition (increase in albumin and decrease in globulin fractions to normal values). A tendency toward an increase in urea concentration to 3.5 ± 0.25 Mm / L was also noted; in control animals, this indicator varied within narrow boundaries of 2.6 ± 0.18 mM / L. Data on enzyme activity indicate a decrease in the cytolytic syndrome (in the experimental group, the level of aminotransferases significantly decreased with a difference of 38% compared with the control). A decrease in the qualitative expression of the thymol test in animals of the experimental group also testified to reparative processes in the liver. Under the influence of Hepavitol, by the end of the experiment, the process was observed to increase the level of vitamins (with high significance of differences): for carotene - 2.5 times and tocopherol - 1.2 times. In the control group, changes in these indicators were insignificant.

Normalization of biochemical parameters during therapy with “Hepavitol” had a more pronounced positive effect on the clinical status of animals. By the end of the experiment, all animals of the experimental group (100%) experienced an increase in the number of scar reduction, pain on palpation of the liver disappeared, its percussion borders returned to normal, the coat became stronger and shinier, bald patches were partially overgrown. In the control group, such changes were recorded only in 18 animals.

From the data presented in table 3, it is seen that the use of the drug contributed to an increase in milk yield. The quality of milk also improved, which was manifested by an increase in the yield of milk protein in animals at the end of the experiment, an increase in the fat content (3.4% versus 3.1%), while the milk contained more dry matter.

The second series of experiments was carried out on pigs (pigs of 2.5-3 months of age) with clinical signs of hepatodystrophy (depression, growth retardation, digestive upset, mucous membrane anemia, liver enlargement, compaction, tousing and yellowness of the bristles), which were confirmed data of a biochemical analysis of blood (a decrease in the protein-synthetic function of the liver and the presence of a cytolytic syndrome).

Animals were formed into 2 groups of 30 animals each. The experimental group of piglets was prescribed "Hepavitol" with a total daily dose of 0.5 ml per kg of body weight daily orally for 30 days, control piglets - vitamin U at a dose of 3-5 mg / kg body weight for three weeks. Otherwise, the feeding and maintenance conditions of the experimental and control piglets were the same.

Studies conducted in dynamics have shown that the use of hepatitis boosted the clinical status of pigs (increased motor activity, feed intake). By the 5th day of treatment in the experimental animals the signs of gastrointestinal tract disorders disappeared, while in the control positive changes occurred only by the 10th day. At the same time, in 9 control piglets, the disease proceeded with complications, which led to their death (mortality was 30%), and in the experimental group there was no death (safety 100%). The data are shown in table 4.

By the end of the experiment, according to the clinical and biochemical parameters, the animals treated with Hepavitol were practically healthy, they had a higher rate of increase in body weight compared to the control (the difference was 42.6%).

Biochemical studies showed that in the pigs of the experimental group, at the end of the observation, metabolic rates improved (the activity of aminotransferases, alkaline phosphatase, cholesterol and bilirubin levels decreased relative to the control analogues, and the concentration of total protein, urea and glucose, on the contrary, increased).

Thus, on the basis of testing, it was found that “Hepavitol” has pronounced therapeutic efficacy in liver diseases in cattle and pigs, while the safety and productivity of animals is significantly increased.

Hepavitol is obtained as follows: sequentially take the constituent components and mix until a homogeneous mass; then packaged in airtight packaging, while it is advisable to provide 2 forms of packaging - for injection and oral administration.

Example 1. In 943.6 g of vegetable deodorized oil was added 0.9 g of beta-carotene, 55 g of phospholipids and 0.5 g of diacetophenanilselenide, mixed. The resulting product was packaged in a sealed container made of dark glass.

Example 2. In 937.2 g of vegetable deodorized oil, 1.8 g of beta-carotene, 60 g of phospholipids and 1.0 g of diacetophenanilselenide were added, mixed. The resulting product was packaged in a sealed container made of dark glass.

Thus, the new tool has a pronounced pharmacological activity, showing therapeutic efficacy in liver diseases in farm animals (rats, pigs), as well as increasing their safety and productivity.

Information sources

1. Antipov V.A., Menshenin V.V., Turchenko A.N., Semenenko M.P., Kuzminova E.V. / Effective zoo veterinary technologies to increase the reproduction, preservation and productivity of animals (guidelines) // Krasnodar, 2005. 79 p.

2. Instructions for use of the drug Hepatovet (registration certificate 32-3-3.0-0181 No. PVR-3-3.0 / 02646 of 01/27/11).

3. RF patent No. 2419420 / Means for improving the safety and productivity of animals // Kuzminova EV, Semenenko MP, Koshchaev AG

4. RF patent No. 2323719 / The drug carcel for the prevention and treatment of obstetric and gynecological diseases in cows // Kazaryan RV, Asvaturyan LK, Lygina EV, Antipov VA, Turchenko A.N ., Kuzminova E.V.

Claims (1)

A remedy for treating liver diseases in cattle and pigs, increasing their safety and productivity, including the target components diacetophenonylselinide (DAFS-25), beta-carotene, vegetable oil and essential phospholipids with the following components, wt. %:
DAFS-25 0.05-0.1 beta carotene 0.09-0.18 phospholipids 5.5-6.0 vegetable oil rest