RU2402902C1 - Method of increasing productivity and safety of cows, calves and piglets - Google Patents

Abstract

FIELD: agriculture.

SUBSTANCE: invention relates to the field of veterinary medicine. The method includes daily administration to animal of preparation containing 0.18-0.24% DAFS-25 (diacetofenonilselenide) and 0.2-0.3% of lycopene, with two courses with an interval of 10-14 days between them. The drug is administered to cows of 40 ml, calves of 20 ml, piglets of 10 ml.

EFFECT: method activates metabolic processes in the body, enables to increase the level of natural resistance of animals and provide increase of their productivity and safety.

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Description

The alleged invention relates to veterinary medicine, namely to the field of preventive and health measures aimed at increasing the safety and productivity of farm animals.

Known methods for increasing the productivity of farm animals when using inorganic compounds of selenium, mainly sodium selenite (Selenium deficiency diseases in animals and birds. Guidelines of the Krasnodar NIVI RAAS. - Moscow. - 2005) and barium selenite (RF patent 2051681, 1996 .), which are prescribed for diseases associated with a deficiency of selenium (white muscle disease, toxic liver dystrophy) and are antioxidants.

The disadvantage of this method is that they use highly toxic forms of selenium with low bioavailability. In addition, these compounds have low solubility, as a result of which they have a slow effect, and barium ions also have high toxicity and often cause local tissue necrosis during injection.

The next negative point is that, when using selenites, animal meat is suitable for food after 45 days after the last injection (V.D. Sokolov et al. Pharmacology, Moscow, 2000).

Also known is a method of increasing productivity and nonspecific resistance of piglets, providing for parenteral administration of a prolonged form of an organic compound of selenium in the form of selenopyran (patent RU 2157690, published on 10/20/2000).

The disadvantage of this method is the low level of effect on the body due to the limited exposure to a single drug, which does not allow for a comprehensive effect and lead to a simultaneous increase in productivity, preservation of farm animals and normalization of metabolic processes in their body.

The closest analogue to the claimed one is a method of correction of metabolic pathology in animals, which consists in the fact that a preparation containing 1 liter of distilled water is administered intramuscularly, g: sodium selenite - 1.0, vitamin E in the form of a combination, E - 7500 mg - tocopherol acetate (which are antioxidants), succinic acid - 10.0, zinc sulfate - 10.0 and pharmazin 50,000 units (Patent RU 2268033 C2).

The disadvantage of this method is that in the formulation of the drug are used substances with a limited effect on various parts of the antioxidant defense of the body. In this case, the use of farmazin, which is an antibiotic from the macrolide group, for the purpose of metabolic correction is not entirely justified, since it is used to treat infections, in other cases, the appearance of resistant strains of microorganisms is a negative manifestation of the pharmacodynamic effects of the drug. It should be borne in mind that macrolides are excreted in milk at a concentration of approximately 20% of its concentration in serum, and slaughter of animals is allowed only 21 days after drug discontinuation (Plamb D.K. Pharmacological preparations in veterinary medicine, Moscow, 2002) .

The objective of the proposed method is to increase the efficiency of normalization and stimulation of biochemical processes of the organism of agricultural animals, manifested by an increase in their safety and productivity.

The solution is achieved by the fact that to increase the productivity and safety of farm animals, a selenium-containing substance is used, characterized in that the animal is administered per os daily once a day for 10 days an oil solution containing 0.18-0.24% DAFS-25 (diacetophenonyl selenide) and 0.2-0.3% lycopene, in two courses with an interval between them of 10-14 days, moreover, the drug is administered to cows in 40 ml, calves in 20 ml, piglets in 10 ml per animal.

The technical result of the proposed proposal is due to the fact that the use of the specified drug according to the proposed scheme has a versatile effect on metabolic processes, including the antioxidant status of the body, in the direction of improving their functional activity, while these dosages and the frequency of use most effectively affect animals .

The use of biologically active substances - vitamins (carotenoid lycopene) and microelements (organo-selenium substance DAFS-25), aims not only to compensate for their deficiency arising from unbalanced feeding, but also to correct biochemical processes at the cellular level, which, in its in turn, provides increased productivity and safety of animals. Moreover, it is the complex use of minerals and vitamins included in the composition of the claimed funds that provides their most effective use, since only in a certain ratio, taking into account their synergism and potentiating action, it is possible to activate many enzyme systems that determine the intensity of metabolic processes. And the more intense the reactions in the body, the higher the level of protective forces and the immune status of animals, the higher their safety and productivity (increase in weight gain, milk yield, milk quality, etc.).

It is known that the body's antioxidant system includes the enzyme system (selenium-dependent glutathione peroxidase, catalase, etc.) and biooxidants (carotenoids, vitamin E, etc.). Thus, the proposed regimen for antioxidant drugs acting at all levels of antioxidant protection ensures the normalization of lipid peroxidation.

In addition to antioxidant properties, carotenoid lycopene has other valuable properties that have a positive effect on the animal organism (anticarcinogenic, antimutagenic, detoxifying and immunostimulating activity), while it is 3 times more active compared to beta-carotene.

Selenium provides the activity of redox enzymes and vitamins, the immunological resistance of the body, and increases the synthesis of the cytochrome P-450 protein. The lack of this trace element in the mother’s body causes her retention of the placenta, infertility, as well as the development of selenium deficiency in the fetus.

In the patent and scientific literature there is no information on the use of combined preparations containing lycopene and the organic form of selenium in order to increase the safety and productivity of farm animals, which suggests the presence of an inventive step in the proposal.

The method is as follows

Testing the effectiveness of the method for assessing its effectiveness was carried out on cows. For this, 24 cows of Aishir breed were selected, which, according to the principle of analogues, were divided into 2 groups (12 animals each). For animals of the first experimental group, the drug was applied per os at 40 ml per individual for 10 days, in two courses with an interval of two weeks. The second group served as a control and did not receive drugs.

In all groups throughout the experiment, the clinical condition of the animals, their safety and productivity were taken into account. Testing results are shown in table 1.

Table 1 Tests to determine the effectiveness of the method on cows Indicators I group - experience II group - control Daily milk yield, kg 18.1 ± 0.5 20.4 ± 0.25 The fat content in milk,% 3.7 ± 0.02 3.85 ± 0.01 Dry matter% 12.76 ± 0.31 13.83 ± 0.21 Total protein,% 3.06 ± 0.05 3.22 ± 0.04 Carotene, mg / ml 0.181 ± 0.012 0.245 ± 0.014

From the data presented in table 1, it is seen that the application of the method contributed to an increase in milk yield by 12.7% and its fat content (3.7% versus 3.85%). Moreover, the milk of the experimental group contained more dry matter, total protein and carotene.

The second series of experiments to evaluate the effectiveness of the method was carried out on two-month-old calves. For this, 3 groups of analogues were formed, 20 individuals in each. In animals of the first experimental group, the drug was administered per os in 20 ml per individual for 10 days, in two courses with an interval of two weeks. The second group of calves (positive control) was injected with sodium selenite at the rate of 0.1 mg / kg feed for 30 days. The third group served as a negative control and did not receive drugs.

In all groups throughout the experiment, the clinical condition of the animals, their safety and productivity were taken into account. Testing results are shown in table 2.

table 2 Comparative tests to determine the effectiveness of the method on calves Indicators At the end of the experiment I group II group III group Number of animals twenty eighteen 17 Preservation,% one hundred 90 85 The average daily weight gain, g 980.6 890.3 840.5

From the data presented in table 2 shows that the safety of calves in the group using the claimed method was 10% higher in comparison with the positive control group and 15% in comparison with animals that did not receive additives. The difference in average daily growth was 10.1% and 16.7%, respectively, in the groups.

The third series of experiments was carried out on pigs of large white breed at the age of 60 days. For this, 3 groups of analogue piglets were formed, each in the machines of 10 individuals. For animals of the first experimental group, the drug was administered per os in 10 ml per individual for 10 days, in two courses with an interval of two weeks. The second group of piglets (positive control) was injected with sodium selenite at the rate of 0.1 mg / kg feed for 30 days. The third group served as a negative control and did not receive drugs (table 3).

In all groups throughout the experiment, the clinical condition of the animals, their safety and productivity were taken into account, and at the end of the experiment, morphobiochemical blood tests were performed.

Table 3 Comparative tests to determine the effectiveness of the method on piglets Indicators At the end of the experiment I group II group III group Number of animals 10 9 7 Preservation,% one hundred 90 70 The average daily weight gain, g 630.5 554.1 538.9

From the data presented in table 3, it is seen that the safety of piglets in the group using the claimed method was 10% higher in comparison with the positive control group and 30% in comparison with animals that did not receive additives. The difference in average daily growth was 13.8% and 16.9%, respectively, in the groups.

Morphological and biochemical studies of the blood of piglets found an increase in metabolic processes in the body of experimental animals, characterized by an increase in hemoglobin, red blood cells, total protein, glucose, calcium and phosphorus, as well as factors of the antioxidant system and natural resistance.

The effectiveness of the method is presented in examples where the components of the drug were taken in the following amounts: organo-selenium substance DAFS-25 (diacetophenonyl selenide) 0.18-0.24%; lycopene 0.2-0.3%; vegetable oil - the rest.

Example 1. Previously, by mixing the organo-selenium substance DAFS-25 (diacetophenonyl selenide) 1.8 g and lycopene 2 g in 996.2 g of vegetable oil, the preparation was obtained. An experimental cow was fed daily with food 1 time per day of the claimed preparation of 40 ml for 10 days, in two courses with an interval of two weeks.

As a result, it was found that the application of the method contributed to an increase in milk yield by 15.8% and its fat content (up to 3.9%). At the same time, milk contained more dry matter, total protein and carotene.

Example 2. According to the method described in example 1, a solution of organo-selenium substance DAFS-25 (diacetophenonyl selenide) 2.4 g and lycopene 3 g in 994.6 g of vegetable oil was prepared. Then the calf daily with food, 1 time per day was given the claimed product in 20 ml for 10 days, in two courses with an interval of two weeks.

Body weight gain was monitored every 10 days and at the end of the experiment. As a result, it was established that during the observation period the animal had no diseases, while there was a high growth rate (average daily weight gain was 1020.8 g).

Example 3. According to the method described in example 1, a solution of organo-selenium substance DAFS-25 (diacetophenonyl selenide) 1.8 g and lycopene 2 g in 996.2 g of vegetable oil was prepared. An experimental piglet was fed daily with food 1 time per day of the claimed preparation of 10 ml for 10 days, in two courses with an interval of two weeks.

Body weight gain was monitored every 10 days and at the end of the experiment. As a result, it was found that during the observation period the animal had no diseases with high growth intensity (average daily weight gain was 690.4 g). Thus, the conducted experimental studies allow us to conclude that the use of an oil solution of an organic form of selenium and lycopene according to the proposed scheme is a highly effective way to increase the productivity and preservation of farm animals.

Claims (4)

1. A method of increasing the productivity and preservation of cows, calves and piglets, including the introduction of selenium-containing preparation, characterized in that, as a selenium-containing preparation, animals are injected with per os oil solution containing 0.18-0.24% DAFS once daily for 10 days -25 and 0.2-0.3% lycopene, and the drug is administered in 10-40 ml, in two courses with an interval between them 10-14 days. 2. The method according to claim 1, characterized in that the drug is administered to cows in a dose of 40 ml. 3. The method according to claim 1, characterized in that the drug is administered to the calves in 20 ml. 4. The method according to claim 1, characterized in that the drug is administered to the piglets in 10 ml.