RU2259832C2 - Preparation for stimulating metabolism and immunity in agricultural animals and birds - Google Patents

Abstract

FIELD: veterinary medicine.

SUBSTANCE: preparation has riboxin, glycolysis activator, vitamin B₁₂, ascorbic acid, citric acid and glucose. The preparation calcium glycerophosphate as the glycolysis activator and additionally has succinic and folic acid and fructose taken in the following ingredient proportions in % by mass: riboxin -10,0-40, calcium glycerophosphate - 30,0-40,0, vitamin B₁₂ - 0.02-0.04, ascorbic acid - 2-4, citric acid - 2-4, succinic acid - 2-4, folic acid - 1-2, fructose 5-10, glucose to 100.

EFFECT: increased laying ability of hens; reduced fodder consumption.

Description

The invention relates to veterinary medicine and can be used in practice for introduction into feed of farm animals and poultry in order to improve feed digestibility, activate growth, increase growth resistance, increase the resistance of young animals and birds to diseases.

The preparation has a powerful source of energy in the form of inosine (riboxin) in high doses, the effect of which is enhanced by the activator of glycolysis - calcium glycerophosphate and a combination of two acids of the tricarboxylic acid cycle - succinic and citric. Ascorbic acid (vitamin C) and vitamin B ₁₂ enhance the body's immune response.

A key problem in agriculture is the preservation of young farm animals and poultry. To a large extent, this problem is specific to animal welfare conditions in Russian farms. It is enough to say that according to published data, the safety of young pigs is 55%, but calf and bird mortality are still high in many farms.

The reasons are weakened offspring with a poorly developed immune system against the background of insufficiently good content. The situation is exacerbated by poorly balanced feed and poor absorption. Attempts to correct the situation with large doses of vitamins do not give a significant effect. Impaired metabolism is the main (90%) cause of the incidence of young animals.

The use of feed antibiotics from 2004 will be banned throughout Europe and is likely to be mandatory for Russia as well.

In this regard, a special role is given to regulatory action supplements, the role of which is to correct impaired metabolism, increase intracellular energy and, as a result, create good immunity from the first days of life.

Currently, there is a fairly large number of additives used for introduction into feed with the above purpose, for example, in patents RU 212207, RU 2125380, RU 2125881, etc.

In addition, there are a significant number of imported supplements with an immunostimulating effect (for example, Orego-Steam from Meriden, Bio-Mos from Altek and others).

As a rule, these additives are extracts from plant, yeast or animal tissues.

There is a patent No. 2195838 C2 (priority from 05/23/2000) "A drug to stimulate growth and increase the resistance of agricultural, domestic animals and poultry" (Purivetin). However, in the course of his extensive production tests, it became clear that it was necessary to strengthen the body's immune response by changing the compositions and increasing the role of individual components.

This goal was achieved as follows:

1. The concentration of inosine (riboxin) is increased by 2 times; methyluracil is excluded from the composition;

2. Instead of PDF (fructose-1,6-diphosphate), the introduction of calcium glycerophosphate has been proposed as a more affordable and, in some cases, more effective substance;

3. Along with citric acid, succinic acid is introduced;

4. Increased concentration of ascorbic acid;

5. In addition to vitamin B ₁₂ , folic acid is included in the composition.

Thus, it was possible to achieve a significant increase in the functions of a number of important body systems:

- Riboxin in combination with succinic and citric acids provides a high metabolic energy of the body, cardiac activity, liver protection, activation of the tricarboxylic acid cycle, protein and nucleic acid synthesis;

- calcium glycerophosphate activates glycolysis and enhances the body's defenses;

- the active functioning of the hematopoietic system is provided by the selected concentrations of vitamin B ₁₂ , ascorbic acid (vitamin C) and folic acid (vitamin Bc).

The created composition was called Purivitin by us.

A method of producing puriovetin in the form of a fine powder consists in mixing the ingredients in a concentration determined at a mixer UZDSP-0.05.

Use components used in medicine and veterinary medicine and having quality certificates.

Preparation of a mixture of components is carried out in several stages:

1. Preparation of filler - crystalline glucose with a moisture content of not more than 10% and a grain size of not more than 1.2 mm. If necessary, glucose is crushed on an MD2-2 hammer mill through a wire mesh with 1.2 mm holes. A portion of this component is produced on an EMB-150 scale.

2. Preparation of a mixture of vitamin B ₁₂ . Vitamin B _{12 is} weighed on an analytical balance of the brand PRI WA-35. Vitamin B _{12 is} mixed with the filler in a ratio of 1:10 in the mixer UZ DSP-0.05 for 3-4 minutes.

3. Submission of macronutrients. Biologically active substances (riboxin, calcium glycerophosphate, ascorbic acid, citric acid, succinic acid, folic acid, fructose) do not require preliminary preparation. The components are weighed on a GW-15 balance; a mixture of B ₁₂ with filler is also weighed on a GW-15 balance.

4. The loading of the mixer is carried out in the sequence: filler, a mixture of vitamin B ₁₂ with filler and macrocomponents. Mixing all the components in the mixers UZ DSP-0.05 is carried out for 4 minutes.

5. After stopping the mixer, the drug is unloaded, packaged and packaged. Storage in a warehouse is carried out at a temperature not exceeding 25 ° C and not lower than 0 ° C. The shelf life of the drug is 1 year.

This drug was extremely effective when used in feed of farm animals and poultry, which is confirmed by the following examples.

Application Examples

The immunostimulating effect of the drug is confirmed:

1. Improving blood counts and weight gain in piglets.

2. Reducing the incidence, increasing the safety of young calves.

Example 1

A study of the effect of the drug on the pig resistance was carried out on the basis of a pig breeding kit of Pobeda JSC in the Leningrad Region.

For the experiments, a preparation of the following composition was used, wt.%:

Riboxin 30.0

Calcium Glycerophosphate 40.0

Vitamin B ₁₂ 0.04

Ascorbic acid 3.0

Citric Acid 2.0

Succinic acid 3.0

Folic Acid 1.5

Fructose 10.0

Glucose 10.96

2 groups of piglets were formed at the age of 2 months, 50 animals each (experience and control), the feeding and keeping conditions of the piglets were the same.

The experimental group of piglets received the drug with food at a dose of 50 mg per 1 kg of live weight once a day for 15 days.

The test results are carried out in tables 1 and 2.

The data obtained show that the drug has a positive effect on the body of piglets, which is manifested in indicators of natural resistance (see table 1).

15 days after the full course of use of the drug, the average daily gain of experimental animals increased by 2 times (see table 2).

Table 1.
Indicators of natural resistance of piglets under the influence of the drug Indicators Units rev. Experience The control Relation to control,% 1 2 3 4 5 Total protein g / l 85.7 ± 0.8 75.5 ± 0.3 113.5 - globulins % 16.0 ± 0.2 8.0 ± 0.4 200,0 Albumin % 62.0 ± 3.1 57.6 ± 2.2 107.6 Lysozyme activity % 58.1 ± 4.2 29.0 ± 5.1 200,0 The ratio of albumin to globulins (a / g coefficient.) 1,5 1.47 102.0

table 2
The effect of the drug on the average daily gain of piglets Indicators Units rev. Experience The control Relation to control,% 1 2 3 4 5 Live weight at the beginning of the experiment kg 4.01 4,5 - Live weight at the end of the experiment kg 7.5 6.24 120.0 Daily gain g 233.0 116.0 200,0

Example 2

The study of the immunostimulating effect of the drug on calves (young cattle) was carried out on the basis of CJSC “Pedigree Plant Civil” of the Leningrad Region in the treatment of sick animals.

For the experiments, a preparation of the following composition was used, wt.%:

Riboxin 35.0

Calcium Glycerophosphate 35.0

Vitamin B ₁₂ 0.03

Ascorbic acid 4.0

Citric Acid 2.0

Succinic acid 3.0

Folic Acid 2.0

Fructose 8.0

Glucose 11.47

2 age groups of calves were formed: group 1 - calves kept in the maternity ward from birth until 15 days of age and group 2 - calves kept in calves from 15 days of age to two months of age. In each group there were experimental and control animals.

Groups of experimental calves received the drug with milk at a dose of 30 mg / kg body weight 1 time per day for 15 days.

Sick calves, in addition to the drug, also received symptomatic treatment.

The experimental results are presented in table 3.

As can be seen from table 3, in the experimental groups the number of cases was 1.5 times less than in the control groups, the loss of young animals was also significantly less in the experimental groups, and there was no waste in the 1st group of calves that received the drug. Thus, the drug increases resistance to diseases (infections), increases the survival of animals.

Table 3.
The effect of the drug on the immune system of calves Units rev. 1 group 2 group experience the control experience the control 1 2 3 4 5 6 The number of calves participating in the experiment Goal. 85 60 250 110 The number of sick calves Goal. 28 54 128 98 The number of sick calves % 32.9 90.0 51,2 89.1 Young growth Goal. 0 5,0 2.0 12.0 Young growth % 0 8.3 0.8 10.9

The drug improves metabolic processes in farm animals and poultry. Confirmation can be, for example, a decrease in feed consumption during rabbit rearing or an increase in feed conversion in laying hens (tables 4 and 5).

Example 3

The study of the effect of the drug on the livelihoods of rabbits was carried out at the Research Institute of fur farming and rabbit breeding them. V.A. Afanasyev (NIIPZK).

For the experiments, a preparation of the following composition was used, wt.%:

Riboxin 40.0

Calcium Glycerophosphate 30.0

Vitamin B ₁₂ 0.04

Ascorbic acid 4.0

Citric Acid 2.0

Succinic acid 4.0

Folic Acid 2.0

Fructose 10.0

Glucose 7.96

2 groups were formed, 29 (control) and 27 (experience) of young rabbits during the period of succession and lactation. The conditions for keeping and feeding both groups were the same.

The rabbits of the experimental group were given a drug with food or drink at a dose of 30 mg per 1 kg of live weight throughout the entire period of succes and lactation. During the feeding of suckling rabbits from birth to 45 days of age, in addition to mother's milk, the drug was additionally given in a dose of 5 mg per 1 kg of live weight.

Table 4 shows the results of the experiments. As can be seen from table 4, the introduction of the drug into the diet of succulent and lactating rabbits increases by 6-10% the live weight of rabbits to be deposited.

The yield of weaning young rabbits to weaning showed a tendency to increase by 30% of this indicator due to the greater, by 19.5%, number of rabbits that were successfully spilled.

In addition, it was shown that the cost of feed (in feed units) per 1 kg of growth in the experiment is 16% lower than in the control.

Table 4
The effect of the drug on the life of rabbits Indicators Units rev. Experience The control Relation to control,% Number of rabbits Goal. 27 29th The number of rabbits that are safely sprinkled % 86.2 66.7 119.5 The average live weight of a rabbit at birth g 56.5 ± 3.6 53.3 ± 5.2 106.0 Average live weight of a rabbit at 20 days of age g 323.7 ± 9.8 300.9 ± 8.0 107.5 45-day-old average rabbit live weight kg 1.3 ± 0.02 1.14 ± 0.02 110 Feed costs per 1 kg of growth kg feed. units 2.1 2.5 84

Example 4

The study of the effect of the drug on the metabolic processes of laying hens was carried out on the basis of the experimental farm VNIIRGZH of the Leningrad region.

For studies, a preparation of the following composition was used, wt.%:

Riboxin 10.0

Calcium Glycerophosphate 30.0

Vitamin B ₁₂ 0.02

Ascorbic acid 2.0

Citric Acid 2.0

Succinic acid 2.0

Folic Acid 1.0

Fructose 5.0

Glucose 48.98

The experiments were carried out on 2 groups of laying hens (experience and control) of the Zarya-17 egg breed, 50 animals in each group.

The cell conditions were the same in both groups of laying hens.

Laying hens of the experimental group received the drug with food in a dose of 110-115 mg per 1 head per day.

The results of the experiments are shown in table 5.

Table 5
The effect of the drug on the absorption of nutrients, productivity and feed conversion Indicators Units rev. Experience The control Relation to control,% Energy % 104 100 Crude protein % 104 100 Fat % 103 100 Crude fiber % 107,2 100 Egg production % 110 100 Feed costs per 100 pcs. eggs % 94.4 100 Feed conversion kg / kg 2.60 2.88 90.0

As can be seen from table 5, with the use of the drug, the absorption of nutrients is increased: crude fiber by 7.2%, energy by 4.0%, crude protein by 4.0%, fat by 3%.

The inclusion of the drug in the feeding diets of laying hens due to increased assimilation of nutrients contributes to an increase in egg production (productivity) by an average of 10%, while feed costs are reduced by 5.6% to receive 100 pcs. eggs and feed conversion improves by 10%.

Claims (2)

1. A preparation for enhancing metabolic processes and immunity in farm animals and poultry, containing riboxin, glycolysis activator, vitamin B ₁₂ , ascorbic acid, citric acid and glucose, characterized in that it contains glycerophosphate as an activator of calcium glycolysis and additionally amber and folic acid and fructose in the following ratio of ingredients, wt.%: Riboxin 10.0-40.0 Calcium Glycerophosphate 30.0-40.0 Vitamin B ₁₂ 0.02-0.04 Vitamin C 2-4 Lemon acid 2 succinic acid 2-4 Folic acid 1-2 Fructose 5-10 Glucose  Up to 100 2. The drug according to claim 1, characterized in that it contains citric and succinic acids in a ratio of 1: 2.