RU2554767C2 - Medication for stimulation of metabolic processes and growth activity of calves - Google Patents

Abstract

FIELD: medicine.

SUBSTANCE: invention relates to veterinary and can be applied in animal-breeding for stimulation of metabolic processes, and growth activity of calves. Medication for stimulation of metabolic processes and growth activity of calves includes succinic acid as energetic stimulator, with application of citric acid as activator of succinic acid, beetroot molasses as carbon component, and methionine and sodium chloride as stimulators of digestion system.

EFFECT: application of invention makes it possible to ensure expressed acceleration of growth energy in early postnatal period.

3 tbl, 2 ex

Description

The invention relates to veterinary medicine and can be used in animal husbandry to stimulate metabolic processes, growth activity, the prevention of gastrointestinal diseases and enhance the overall resistance of the body of newborn animals.

The issues of ensuring the safety of newborn animals and increasing the resistance of young animals are problematic tasks in veterinary science and practice. The current intensification of animal husbandry has led to a number of negative factors that have an extremely negative effect on the health status of newborns. The born offspring initially has defects in the metabolic and immune systems, which is manifested by malnutrition, stunted growth and development, immunodeficiencies, and high rates of gastrointestinal diseases.

For the prevention and treatment of the above pathophysiological conditions and diseases in newborns, various stimulants of metabolic and immune processes, chemo and antibiotic therapy are widely practiced. In the vast majority of these funds are used as single drugs. The narrow focus of these funds often does not give a positive result when they are used in the system of ensuring the health and safety of newborns.

Currently, in medicine and veterinary medicine are widely used drugs of metabolic action based on succinic acid.

The absolute harmlessness of succinic acid and its derivatives, the ability to have a positive effect even at absolutely low dosages, to enhance the effect of other drugs, make it very valuable in the development of complex preparations, feed and food additives.

The closest in composition drug for enhancing the metabolic processes of immunity in farm animals, which we have chosen as a prototype, is Purivetin (RF patent No. 2259832 of 09/10/2005). The composition of the drug Purivetin includes the following components: weight%: riboxinum - 10.0-40.0; calcium glycerophosphate 30.0-40.0; vitamin B 12-0.02-0.04; ascorbic acid 2-4; citric acid-2; succinic acid 2-4; folic acid 1-2, fructose 5-10, glucose up to 100.

The inclusion of the drug in the diet of animals and birds was very effective. The drug significantly improves metabolic processes, increases resistance to infectious diseases.

However, the preparation contains components, in particular glucose, fructose, which are considered quite deficient, which significantly limits its release.

The objective of the present invention is to obtain a preparation for stimulating metabolic processes and growth activity of calves of the milk growing period.

To solve this problem, a drug with energy-metabolic activity is proposed.

The main energy component in the drug is succinic acid. Succinic acid is a powerful stimulator of energy production in cells. As you know, the digestion process is associated with significant energy costs. In this regard, succinic acid, providing energy to the cells of the gastric mucosa, improves digestion. As a result, protein, mineral and vitamin substances contained in milk are more fully used.

Succinic acid and its salts have a wide spectrum of effects on various mechanisms of regulation of the metabolic activity of cells, which is especially important in various pathophysiological conditions. The object of the action of UC are cells and tissues that are in a state of excitement or pathologically altered. The stimulating effect of succinic acid is especially pronounced with weakening of the body and disease.

The absolute harmlessness of UC and its compounds, which have the ability to have a positive effect at the lowest dosages, make it very attractive when developing a new generation of so-called “smart” drugs (Kondrashova MN, 1996).

To enhance the pharmacological effect of succinic acid, citric acid is introduced into the composition. Citric acid is an activator of succinic acid in the Krebs tricarboxylic acid cycle. The complex compound of succinic and citric acids is implemented in the well-known tabletted preparation Limontar (RF Patent No. 2039556).

As a carbohydrate nutrition and partially to correct the unpleasant taste of succinic acid, beet molasses is included in the preparation. Beet molasses is widely used in industrial animal husbandry as a source of carbohydrates, improving the taste of feed.

To enhance metabolic and hepatoprotective activity, methionine is included in the composition of the drug. Methionine is an essential amino acid and is a “critical” amino acid for newborn animals, because their digestive system cannot synthesize it from nitrogen-containing feed substances. With a lack of methionine, anemia, fatty and toxic liver dystrophy are often observed. In the body, methionine activates the action of a number of enzymes, hormones, vitamin B ₁₂ , folic and ascorbic acids. Many processes of neutralization of toxins in the liver also occur with the participation of methionine.

As a means of improving digestion, sodium chloride is included in the preparation in an amount of 2% of the volume. Metabolism of sodium chloride is closely associated with metabolic processes impaired in diarrhea, metabolic acidosis. These basic pathophysiological conditions, to one degree or another, are observed in calves at an early age.

The proposed composition allows to obtain metabolic, energy-stimulating effects.

The quantitative content of the components providing the above effects was found experimentally and is illustrated by the following experiment.

For the experiment, experimental series of compositions containing 100 ml of solution were prepared:

- 1 g of succinic acid, 2 g of sodium chloride and 30 ml of beet molasses (according to the recipe 1);

- 1 g of succinic acid, 2 g of sodium chloride, 30 ml of beet molasses, 1 g of methionine (according to prescription 2);

- 1 g of succinic acid, 2 g of sodium chloride, 30 ml of beet molasses, 1 g of methionine, 0.1 g of citric acid (according to prescription 3);

- 1 g of succinic acid (control);

Growth activity was assessed in mice with initial body weight of 10–11 g. In each group, there were 5 mice. The above formulations were administered orally, forcibly, in a volume of 1 ml, three times with an interval of 24 hours. After 10 days, the experimental mice were weighed.

The weighing results are presented in table 1.

The results of the experiment indicate a marked increase in growth activity when included in the composition of succinic acid and molasses.

The technology for producing the metabolic composition is illustrated by the following examples.

Example 1. A sample containing 10 g of succinic acid and 2 g of sodium chloride is introduced into a flask with 200 ml of pure tap water. Warm up until the succinic acid is completely dissolved, after which 4 g of methionine are added. At the same time, 500 ml of pure tap water is poured into enameled dishes. 300 ml of beet molasses heated to 30-35 ^o C are poured into it. The contents of the flask are poured into a solution with beet molasses. Mix thoroughly, packaged in plastic bottles for food purposes with a capacity of 0.5 l, sealed with caps.

Example 2. The same technology is used as in example 1, adding 1 g of citric acid to a sample of succinic acid.

The obtained drug variants, designated by us as “amber biostimulant” for oral administration, were tested on newborn calves of 2-3 days of age with clinically expressed symptoms of malnutrition.

Assessment of the effect of the drug on the stimulation of growth activity, hematological and biochemical processes in hypotrophic calves.

In comparative testing, three compositional options were tested. Composition according to the recipe 1 (succinic acid - 5 g, sodium chloride 10 g, beet molasses - 150 ml); composition according to the recipe 2 (succinic acid-5 g, sodium chloride 10 g, beet molasses - 150 ml, methionine - 2 g); composition according to the recipe 3 (succinic acid-5 g, sodium chloride-10 g, citric acid - 0.5 g, beet molasses - 150 ml, methionine - 2 g); control - composition according to the recipe 4 (succinic acid - 5 g). These formulations were prepared based on 500 ml of the solution and were evaporated for three consecutive days, 500 ml at a time, once a day. A similar group of hypotrophic calves served as control. Metabolic activity was evaluated according to the results of studies of blood serum according to the main hematological and biochemical parameters.

The research results are presented in table 2.

According to the results of the biochemical test, a more pronounced effect of formulations 2 and 3 (with the addition of methionine) on hematopoiesis (the number of red blood cells and their saturation with hemoglobin), protein metabolism and mineral metabolism as compared to the control and composition without methionine was established.

Stimulation of metabolic processes favorably reflected in the growth energy (table 3).

Thus, based on a series of experimental experiments, it was found that the test composition based on beet molasses and succinic acid with the addition of methionine, citric acid and sodium chloride provides a pronounced acceleration of growth energy in the early postnatal period.

Table 1 The influence of metabolic compositions on the growth activity of mice A drug average weight of mice (g) background after 10 days yak + beet molasses 10.4 + 0.2 17.9 + 0.4 yak + beet molasses + methionine 10.4 + 0.3 18.2 + 0.4 yak + beet molasses + methionine + citric acid 10.3 + 0.2 18.4 + 0.2 yak (control) 10.4 + 0.2 13.1 + 0.4

table 2 The influence of the tested compounds on the metabolic processes of calves hypotrophic A drug Hemoglobin, g / l Red blood cells, million / cm ³ Protein, g / l Calcium mmol / L Phosphorus mmol / L By recipe 1 78.24 ± 3.12 85.6 ± 2.36 3.9 ± 0.4 4.4 ± 0.2 61.8 ± 2.4 65.7 ± 2.2 1.56 ± 0.11 2.78 ± 0.15 1.29 ± 0.16 1.62 ± 0.23 According to the recipe 2 79.62 ± 2.75 89.19 ± 2.15 3.9 ± 0.2 4.6 ± 0.1 61.2 ± 2.3 67.3 ± 2.3 1.54 ± 0.09 2.67 ± 0.12 1.31 ± 0.12 1.65 ± 0.18 According to the recipe 3 78.56 ± 2.84 90.26 ± 2.54 3.9 ± 0.3 4.6 ± 0.2 61.4 ± 2.5 70.2 ± 2.3 1.54 ± 0.06 2.82 ± 0.14 1.33 ± 0.14 1.67 ± 0.19 The control
yak 78.56 ± 2.42 82.8 ± 2.56 3.9 ± 0.2 4.2 ± 0.3 60.8 ± 2.4 62.7 ± 2.6 1.59 ± 0.11 1.67 ± 0.08 1.34 ± 0.17 1.58 ± 0.13 Note: numerator - background indicators; denominator - indicators for 10 days

Table 3 The influence of the tested formulations of the drug on the stimulation of the growth activity of hypotrophic calves Group A drug Body weight (kg) Daily gain (g) At the beginning of the experiment 14 days later one By recipe 1 22.5 ± 2.3 32.4 ± 2.7 720 2 According to the recipe 2 23.1 ± 2.4 33.5 ± 3.1 780 3 According to the recipe 3 23.1 ± 2.5 34.1 ± 2.4 790 four The control 24.5 ± 2.3 30.8 ± 2.9 490

Claims (1)

A preparation for stimulating the metabolic processes and growth activity of calves, including succinic acid as an energy stimulant, characterized in that citric acid is used as an activator of succinic acid, beet molasses as a carbohydrate component, methionine and sodium chloride are stimulants of the digestive system, in the following ratio of components based on 0.5 liter of solution
Succinic acid 5.0 g
Citric acid 0.5 g
Beet molasses 150.0 ml
Methionine 2.0 g
Sodium chloride 10.0 g
Water is the rest.