RU2555005C2 - Energometabolic composition for stimulation of exchange processes, prevention of hypomicroelementosis and diarrhea in piglets - Google Patents

Abstract

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention relates to veterinary and can be applied in pig-breeding for the stimulation of exchange processes, growth activity, prevention of hypomicroelementosis and diarrhoeas of piglets. An energometabolic composition for the stimulation of exchange processes represents a water solution, which contains succinic acid, iodinol, beet molasses and sulphates of iron, copper, cobalt, zinc, with the following component content in 1000 ml of the solution: succinic acid - 10.0 g, iodinol - 250 ml, beet molasses - 300 ml, iron sulphate - 2 g, copper sulphate - 0.1 g, cobalt sulphate - 1 g, zinc sulphate - 0.3 g, water- the remaining part.

EFFECT: application of the invention will make it possible to improve exchange processes in piglets.

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Description

The invention relates to veterinary medicine and can be used in pig farming to stimulate metabolic processes, growth activity, prevention of hypomicroelementoses and diarrhea in piglets.

Currently, in medicine and veterinary medicine are widely used drugs of metabolic action based on succinic acid.

The absolute harmlessness of succinic acid and its derivatives, the ability to have a positive effect even at absolutely low dosages, to enhance the effect of other drugs, make it very valuable in the development of complex preparations, feed and food additives.

Known anti-anemic and growth-promoting drug for animals - Gemovit-M (RF Patent No. 2097045, 1997). The preparation includes the following components, wt.%: Disodium or dipotassium salt of ethylenediamine-N, N'-disuccinic acid 15-25; iron (III) 3.0-5.0; manganese (II) 0.5-2.5; copper (II) 0.25-0.5; cobalt (II) 0.1-0.3; zinc (II) 0.1-1.5; water is the rest. The drug is recommended to eliminate the deficiency of trace elements, normalize metabolism, increase the reproductive abilities of females, stimulate the growth of animals.

Known drug "Gemovit-plus", comprising the following components, wt.%: Disodium or dipotassium salt of ethylenediamine-N, N1-disuccinic acid 15-25; iron (III) 0.6-5.0; manganese (II) 0.5-2.0; copper (II) 0.05-0.25; zinc (II) 0.1-1.5; cobalt (II) 0.005-0.05; selenium (IV) 0.01-0.03; iodine (I) 0.01-0.05; the rest is water (RF Patent No. 2203657, 2003). The drug eliminates the deficiency of trace elements, stimulates erythropoiesis, and increases the nonspecific resistance of the body. The use of the drug enhances reproductive ability, stimulates the growth, development and productivity of farm and domestic animals.

The inclusion of the above drugs in the diet of animals has been very effective. They significantly improve metabolic processes, prevent hypomicroelementoses. However, the low palatability of the components that make up the drug when added to the feed reduces the appetite of animals.

The objective of the present invention was to obtain a complex composition with energy-metabolic, anti-anemic and antidiarrheal activity.

To solve this problem, a composition is proposed, including succinic acid, beet molasses, vital trace elements and iodinol.

The main energy component in the drug is succinic acid (UC). UC is a powerful stimulator of energy production in cells, which is especially important in all kinds of pathophysiological conditions, when the body does not have enough energy for the normal provision of vital functions. UC and its salts have a wide range of effects on various mechanisms of regulation of the metabolic activity of cells. The object of the action of UC are cells and tissues that are in a state of excitement or pathologically altered. The stimulating effect of succinic acid is especially pronounced when weakening the body and its disease.

As a carbohydrate nutrition and to correct the unpleasant taste of succinic acid and trace elements, beet molasses is included in the composition of the drug. Beet molasses is widely used in industrial animal husbandry as a source of carbohydrates, improving the taste of feed.

For the prevention of hypomicroelementoses, the composition includes salts of macro- and microelements: iron sulfate, copper sulfate, cobalt sulfate, zinc sulfate.

Iron sulfate. Iron is involved in hematopoiesis, is part of hemoglobin, and increases the overall resistance of the body. Iron sulfate is prescribed to improve digestion.

Copper sulfate. Copper, like iron, is involved in the processes of hematopoiesis, osteogenesis, is part of the enzyme. Copper accelerates the transition of iron from inorganic forms to organic. This favorably affects the processes of hematopoiesis, especially with alimentary anemia.

Cobalt sulfate. Cobalt affects the hematopoietic functions of the bone marrow, increases the level of hemoglobin and the number of red blood cells, increases the overall resistance.

Zinc sulfate. Zinc stimulates hematopoiesis, the exchange of nucleic acids, proteins, carbohydrates, the growth and development of animals.

As an anti-infectious component, iodinol is included in the composition. Iodinol and its medical analogue “blue iodine” have pronounced antimicrobial activity, have long been used in gastrointestinal diseases of infectious etiology. The drug iodinol is well understood. For oral administration to piglets, it is recommended in an amount of 3-25 ml. The resistance of microorganisms to iodine preparations is not developed (IE Brains Pharmacology. "Spike", 1974. - S.290-292).

The activity of the complex of trace elements is enhanced by the action of succinic acid. Succinic acid, as a regulator of metabolic processes, reduces the risk of an overdose of any trace element.

The proposed composition allows you to get both metabolic, antianemic and antidiarrheal effects.

The quantitative content of the components providing the above effects were found experimentally and are illustrated by the following experiment.

For the experiment, experimental series of compositions containing 100 ml of solution were prepared:

- 1 g of succinic acid, 30 ml of molasses and 200.0 mg of iron sulfate (according to prescription 1);

- 1 g of succinic acid, 30 ml of molasses and 10.0 mg of copper sulfate (according to prescription 2);

- 1 g of succinic acid, 30 ml of molasses and 100.0 mg of cobalt sulfate (according to prescription 3);

- 1 g of succinic acid, 30 ml of molasses and 10.0 mg of zinc sulfate (according to prescription 4);

- 1 g of succinic acid, 30 ml of molasses and 25.0 ml of iodinol (according to the prescription 5).

In parallel, similar solutions without molasses were prepared. They were used as a control.

Growth activity was assessed in mice with initial body weight of 10–11 g. In each group, there were 5 mice. The above formulations were administered orally, forcibly, in a volume of 1 ml, three times with an interval of 24 hours. After 10 days, the experimental mice were weighed. The weighing results are presented in table 1.

The experimental results indicate a marked increase in growth activity when the molasses is included in the composition with succinic acid. This pattern also manifested itself when it was included in the composition of the mono- and complex metal salts.

When assessing the toxicity of a composition containing the above quantitative complex of metal salts, it was found that LD ₅₀ ranges from 24,000 to 25,000 mg / kg body weight. In contrast, a composition with a similar concentration of metal salts without succinic acid had an LD ₅₀ in the range of 49000-50000 mg / kg body weight.

Assessment of anti-infective activity. To assess anti-infective activity, a composition was used, including iodinol (according to prescription 3). As a comparison, a similar concentration of iodinol and saline was used. In each group there were 7 mice. The average weight of mice is 19-21 g. For infection, a pre-titrated pathogenic culture of E. coli was used, causing the death of at least 50% of individuals (LD ₅₀ ) with the oral infection method. An hour after infection, the experimental mice were administered twice with an interval of 3 hours in a volume of 1.0 orally containing formulations containing 3% iodinol (the first group of mice); according to the registration No. 5; (second group); saline (control group). Clinical observation of experimental mice was conducted for 5 days. The results of the experiment are presented in table 2.

According to the results of the experiment, it was found that the prescription 5 drug provided a higher level of protection for mice compared to a similar concentration of iodinol.

The method of obtaining energy metabolism composition

Upon receipt of the energy-metabolic composition, weighed portions are prepared sequentially containing 10 g of succinic acid, 2.0 g of iron sulfate, 100.0 mg of copper sulfate, 1.0 g of cobalt sulfate, 100.0 mg of zinc sulfate. Weighed portions are introduced into a flask with 180.0 ml of pure tap water and heated with stirring until the metal salts and succinic acid are completely dissolved. After that, the contents of the flask are poured into an enameled container in which 300 ml of molasses and 250 ml of iodinol are previously dissolved in 250 ml of tap water. Water is added to 1000 ml of the solution. The composition is mixed.

The resulting composition was tested in two scientific and production experiments. The place of experiments is the pig farm "Magnetic" of the Kursk region.

Experience 1. Evaluation of the influence of the tested compounds on the hematological and biochemical processes of hypotrophic piglets.

From the number of hypotrophic piglets of 20 days of age, three experimental groups of 7 individuals were formed.

The first group of piglets, within 14 days, with free access, instead of water, the claimed composition was evaporated. The second group was evaporated composition based on succinic acid and molasses. The third group (n = 8) was the control.

The influence of the tested compounds on the hematological and biochemical processes of hypotrophic piglets is presented in table 3.

The influence of the tested compounds on the stimulation of the growth activity of hypotrophic piglets is presented in table 4.

Experience 2. Evaluation of the use of the composition for the prevention of diarrhea in piglets of an early growing period.

In testing used the inventive composition and composition, including a 1.0% aqueous solution of succinic acid.

The experiment included piglets of 5 days of age. The somewhat "belated" summer residence of the tested compounds was due to the fact that in the early suction period, mother’s milk is enough for them to provide growth energy. However, from 5 days of age, the nutritional needs of piglets are no longer satisfied with breast milk. This circumstance was taken into account.

The use of formulations for soldering was carried out with free access to piglets. In the course of observations it was found that the piglets with great desire used the composition with molasses.

The effect of the tested compounds on the incidence of suckling piglets with diarrhea is presented in table 5.

Thus, based on a series of experimental experiments, it was found that the tested compositions based on molasses and succinic acid with the inclusion of trace elements provide a pronounced acceleration of growth energy, and the addition of iodinol helps to prevent gastrointestinal diseases.

Table 1 The influence of metabolic compositions on the growth activity of mice A drug The average mass of mice (g) background after 10 days yak + molasses + iron 10.2 ± 0.3 17.6 ± 0.3 yak + iron 10.3 ± 0.2 15.3 ± 0.2 yak + molasses + copper 10.3 ± 0.2 17.7 ± 0.4 yak + copper 10.4 ± 0.2 15.2 ± 0.3 yak + molasses + cobalt 10.4 ± 0.3 17.4 ± 0.2 yak + cobalt 10.3 ± 0.3 15.2 ± 0.3 yak + molasses + zinc 10.3 ± 0.2 17.8 ± 0.3 yak + zinc 10.2 ± 0.3 15.5 ± 0.2 yak + molasses + iodinol 10.3 ± 0.3 17.5 ± 0.2 yak + iodinol 10.2 ± 0.4 15.3 ± 0.2 yak + molasses + trace elements 10.4 ± 0.5 18.2 ± 0.3 yak + trace elements 10.4 ± 0.3 16.9 ± 0.3

table 2 Assessment of anti-infective activity Structure The death of mice in days Survival 1-3 4-5 % Iodinol four 42.9 According to the registration 5 3 57.1 Fiz. solution 5 28.6

Table 3 The influence of the tested compounds on the hematological and biochemical parameters of the blood of piglets Indicators Background metrics 14 days 22 days Red blood cells, 10 ¹² / L 4.8 ± 0.2 6.2 ± 0.2 * 4.8 ± 0.3 * 4.9 ± 0.3 5.6 ± 0.3 * 4.6 ± 0.3 * 4.8 ± 0.2 4.8 ± 0.2 3.9 ± 0.2 Hemoglobin, g / l 78.56 ± 2.84 95.37 ± 4.94 * 119.72 ± 5.56 * 78.53 ± 3.59 88.15 ± 3.64 * 102.42 ± 4.18 * 79.02 ± 3.26 79.68 ± 2.75 79.24 ± 2.27 Protein, g / l 52.3 ± 2.2 58.3 ± 3.1 69.2 ± 3.2 53.1 ± 2.7 57.0 ± 2.9 57.8 ± 3.6 53.7 ± 2.4 53.5 ± 2.6 54.1 ± 3.1 Total calcium, mmol / l 1.54 ± 0.10 2.08 ± 0.12 * 2.45 ± 0.14 * 1.59 ± 0.12 1.84 ± 0.19 2.15 ± 0.11 * 1.48 ± 0.18 1.53 ± 0.11 1.57 ± 0.18 Inorganic phosphorus, mmol 1.29 ± 0.16 1.52 ± 0.12 1.82 ± 0.12 * 1.32 ± 0.07 1.46 ± 0.12 1.72 ± 0.14 1.31 ± 0.12 1.33 ± 0.14 1.36 ± 0.15 Reserve alkalinity, vol% CO2 19.8 ± 1.6 29.2 ± 2.1 * 35.7 ± 3.1 * 21.1 ± 1.2 29.3 ± 2.7 * 34.9 ± 3.9 * 19.9 ± 1.7 20.7 ± 1.3 22.6 ± 1.7 Iron, μmol / L 11.6 ± 1.9 21.7 ± 2.1 * 18.3 ± 1.9 10.4 ± 1.5 12.6 ± 1.8 13.2 ± 1.7 11.6 ± 1.3 12.1 ± 1.4 12.6 ± 1.3 Copper, µmol / L 15.7 ± 2.1 16.7 ± 2.05 16.9 ± 1.81 15.9 ± 1.47 16.3 ± 1.56 16.5 ± 1.72 15.4 ± 1.35 15.5 ± 1.42 15.7 ± 1.48 Cobalt, μmol / L 0.43 ± 0.11 0.64 ± 0.12 * 0.67 ± 0.14 * 0.48 ± 0.10 0.49 ± 0.11 0.51 ± 0.12 0.44 ± 0.11 0.42 ± 0.10 0.43 ± 0.11 Zinc, micromol / l 41.3 ± 2.52 44.5 ± 3.19 46.3 ± 3.32 * 41.6 ± 3.09 42.3 ± 2.57 43.4 ± 2.46 41.7 ± 3.06 40.3 ± 2.44 39.2 ± 2.35 Note: the top line is the indicators for piglets of the first group; middle row - indicators in piglets of the second group; top line - indicators in piglets of the control group; p <0.05 * in relation to the control group

Table 4 The influence of the tested compounds on the stimulation of the growth activity of hypotrophic piglets Group Structure Body weight (kg) Daily gain (g) At the beginning of the experiment 15 days later one The inventive composition 5.6 + 0.3 9.7 + 0.4 273 2 Yak + molasses 5.8 + 0.4 9.5 + 0.3 246 3 The control 5.7 + 0.3 8.2 + 0.3 166

Table 5 The influence of the tested compounds on the incidence of suckling piglets with diarrhea Structure Number of piglets Registration of cases of diarrhea in the period (number /%) 5-10 days 11-16 days 17-22 days The inventive composition 28 2 (6.1) - 1 (3.6) Amber acid solution 27 5 (18.9) 4 (14.8) 2 (7.4) The control 28 18 (64.2) 16 (57.1) 10 (35.6)

Claims (1)

Energy-metabolic composition for the stimulation of metabolic processes, the prevention of hypomicroelementosis and diarrhea of piglets in the form of an aqueous solution, including trace elements and an iodine-containing component, characterized in that it additionally contains succinic acid and beet molasses in the following ratio of components per 1000 ml of solution:
Succinic acid - 10.0 g
Iodinol - 250 ml
Beet molasses - 300 ml
Iron sulfate - 2 g
Copper sulfate - 0.1 g
Cobalt sulfate - 1 g
Zinc sulfate - 0.3 g
Water is the rest.