RU2521369C1 - Agent or stimulation of metabolic processes, prevention and treatment of gastrointestinal diseases in calves in early postnatal period - Google Patents

Abstract

FIELD: veterinary medicine.

SUBSTANCE: agent or stimulation of metabolic processes, prevention and treatment of gastrointestinal diseases in calves in early postnatal period comprises succinic acid as the energy stimulant, beet molasses as carbohydrate component, methionine as the stimulant of the digestive system, iodinol as the anti-infective component, and water in the following ratio of components, based on 0.5 litre: succinic acid 5.0 g; beet molasses 150.0 ml; methionine 2.0 g; iodinol 30-50 ml; water - the rest.

EFFECT: improvement of agent efficiency.

5 tbl, 2 ex

Description

The invention relates to veterinary medicine and can be used in animal husbandry to stimulate metabolic processes, growth activity, the prevention of gastrointestinal diseases and enhance the overall resistance of the body of newborn animals.

The issues of ensuring the safety of newborn animals and increasing the resistance of young animals are problematic tasks in veterinary science and practice. The current intensification of animal husbandry has led to a number of negative factors that have an extremely negative effect on the health status of newborns. The born offspring initially has defects in the metabolic and immune systems, which is manifested by malnutrition, stunted growth and development, immunodeficiencies, and high rates of gastrointestinal diseases.

For the prevention and treatment of the above pathophysiological conditions and diseases in newborns, various stimulants of metabolic and immune processes, chemo and antibiotic therapy are widely practiced. In the vast majority of these funds are used as single drugs. The narrow focus of these funds often does not give a positive result when they are used in the system of ensuring the health and safety of newborns.

Currently, in medicine and veterinary medicine are widely used drugs of metabolic action based on succinic acid.

The absolute harmlessness of succinic acid and its derivatives, the ability to have a positive effect even at absolutely low dosages, to enhance the effect of other drugs make it very valuable in the development of complex preparations, feed and food additives.

The closest composition of the drug to enhance the metabolic processes of immunity in farm animals, which we have chosen as an analogue, is Purivetin (RF patent No. 2259832 of 09/10/2005). The composition of the drug Purivetin includes the following components: wt.%: Riboxin 10.0-40.0; calcium glycerophosphate 30.0-40.0; Vitamin B12 0.02-0.04; ascorbic acid 2-4; citric acid 2; succinic acid 2-4; folic acid 1-2, fructose 5-10, glucose up to 100.

The inclusion of the drug in the diet of animals and birds was very effective. The drug clearly improves metabolic processes, increases resistance to infectious diseases.

However, the preparation contains components, in particular glucose, fructose, which are considered quite deficient, which significantly limits its release.

The objective of the present invention was to obtain a drug for the prevention and treatment of metabolic disorders, gastrointestinal diseases, enhancing detoxification function of the liver, increasing the growth activity of animals of the early postnatal period.

To solve this problem, a drug is proposed for stimulating metabolic processes, preventing and treating gastrointestinal diseases of calves in the early postnatal period, including succinic acid as an energy stimulator, using beet molasses as a carbohydrate component, methionine as a stimulant of the digestive system, and as an anti-infectious component - iodinol, and water, in the following ratio of components based on 0.5 liter:

Succinic acid - 5.0 g

Beet molasses - 150.0 ml

Methionine - 2.0 g

Iodinol - 30-50 ml

Water is the rest.

The main energy component in the drug is succinic acid. Succinic acid is a powerful stimulator of energy production in cells, which is especially important in all kinds of pathophysiological conditions, when the body does not have enough energy for the normal provision of vital functions. Succinic acid and its salts have a wide range of effects on various mechanisms of regulation of the metabolic activity of cells. The object of the action of UC are cells and tissues that are in a state of excitement or pathologically altered. The absolute harmlessness of UC and its compounds, which have the ability to have a positive effect at the lowest dosages, make it very attractive when developing a new generation of so-called “smart” drugs (Kondrashova MN, 1996).

The stimulating effect of succinic acid is especially pronounced when weakening the body and its disease.

As a carbohydrate nutrition, beet molasses is included in the preparation. Beet molasses is widely used in industrial animal husbandry as a source of carbohydrates, improving the taste of feed.

As an anti-infectious component, iodinol is used. Iodinol and its medical analogue “blue iodine” have pronounced antimicrobial activity, have long been used in gastrointestinal diseases of infectious etiology. The drug iodinol is well understood. For oral administration to calves, it is recommended in an amount of 25-30 ml. The resistance of microorganisms to iodine preparations is not developed (IE Mozgov Pharmacology. "Spike", 1974. - S.190-192). There are no data on the use of succinic acid, molasses and iodinol in a single composition.

To enhance metabolic and hepatoprotective activity, the inclusion of methionine is possible in the composition of the drug. Methionine is an essential amino acid and is a “critical” amino acid for newborn animals, since their digestive system cannot synthesize it from nitrogen-containing feed substances. With a lack of methionine, anemia, fatty and toxic liver dystrophy are often observed. In the body, methionine activates the action of a number of enzymes, hormones, vitamin B ₁₂ , folic and ascorbic acids. Many processes of neutralization of toxins in the liver also occur with the participation of methionine.

The proposed composition allows you to get both metabolic, energy-stimulating and anti-infectious effects.

The quantitative content of the components providing the above effects were found experimentally and are illustrated by the following experiment.

For the experiment, experimental series of compositions containing 100 ml of solution were prepared:

- 1 g of succinic acid and 30 ml of beet molasses (according to the recipe 1);

-1 g of succinic acid, 30 ml of beet molasses, 1 g of methionine (according to prescription 2);

- 1 g of succinic acid, 30 ml of beet molasses and 3 ml of iodinol (according to prescription 3);

- 1 g of succinic acid (control).

Growth activity was assessed in mice with initial body weight of 10–11 g. In each group, there were 5 mice. The above formulations were administered orally, forcibly, in a volume of 1 ml, three times with an interval of 24 hours. After 10 days, the experimental mice were weighed. The weighing results are presented in table 1.

The results of the experiment indicate a marked increase in growth activity when included in the composition of succinic acid and beet molasses.

Assessment of anti-infective activity.

To assess anti-infective activity, a composition was used, including iodinol (according to prescription 3). As a comparison, a similar concentration of iodinol and saline was used. In each group there were 7 mice. The average weight of mice is 19-21 g. For infection, a pre-titrated pathogenic culture of E. coli was used, causing the death of at least 50% of LD ₅₀ individuals) by the oral method of infection. An hour after infection, the experimental mice were administered twice with an interval of 3 hours in a volume of 1.0 orally containing formulations containing 3% iodinol (the first group of mice); according to the registration No. 3; (second group); saline (control group). Clinical observation of experimental mice was conducted for 5 days. The results of the experiment are presented in table 2.

According to the results of the experiment, it was found that the preparation according to prescription 2 provided a higher level of protection for mice compared to a similar concentration of iodinol.

The technology for producing the drug is illustrated by the following examples.

Example 1. A sample containing 10 g of succinic acid is introduced into a flask with 200 ml of pure tap water. Warm up until the succinic acid is completely dissolved, after which 4 g of methionine are added. At the same time, 500 ml of pure tap water is poured into enameled dishes. 300 ml of beet molasses heated to 30-35 ° C are poured into it. The contents of the flask are poured into a solution with beet molasses. Mix thoroughly, packaged in plastic bottles for food purposes with a capacity of 0.5 l, sealed with caps.

Example 2. Upon receipt of the drug with anti-infective activity, the same approaches are used as in example 1. The amount of water is reduced by 60 ml, replacing it with a similar amount of iodinol.

The obtained preparation, designated by us as “amber biostimulant” for oral administration, was tested on newborn calves of 2-3 days of age with clinically expressed symptoms of diarrhea and malnutrition.

Assessment of the effect of the drug on the stimulation of growth activity, hematological and biochemical processes in hypotrophic calves.

In comparative testing, two variants of the composition were tested. Composition according to the recipe 1 (succinic acid - 5 g, beet molasses - 150 ml); composition according to the recipe 2 (succinic acid - 5 g, beet molasses - 150 ml, methionine - 2 g). These formulations were evaporated three days in a row at 500 ml at a time, once a day. A similar group of hypotrophic calves served as control. Metabolic activity was evaluated according to the results of studies of blood serum according to the main hematological and biochemical parameters.

The research results are presented in table 3.

According to the results of the biochemical test, a more pronounced effect of composition 2 (with the addition of methionine) on hematopoiesis (the number of red blood cells and their saturation with hemoglobin), protein metabolism and mineral metabolism compared with the control and composition without the addition of methionine was established. Stimulation of metabolic processes favorably reflected in the growth energy (table 4).

Evaluation of antidiarrheal efficacy.

Evaluation of antidiarrheal efficacy was performed on calves using two formulations: at a single dacha, composition 1 included (succinic acid - 5 g, beet molasses - 150 ml, 350 ml of water, methionine - 2 g); composition 2 (succinic acid - 5 g, beet molasses - 150 ml, 320 ml of water, iodinol - 30 ml).

In the first experiment, the assessment was carried out on newborn calves with severe symptoms of diarrhea without signs of intoxication.

The above formulations were evaporated by force, replacing the next milk drink, until the diarrhea stopped.

The results of the experiment are presented in table 5.

Thus, based on a series of experimental experiments, it was found that the tested formulations based on beet molasses and succinic acid provide a pronounced acceleration of growth energy and the prevention of gastrointestinal diseases of calves in the early postnatal period.

Table 1 The influence of metabolic compositions on the growth activity of mice A drug average weight of mice (g) background after 10 days yak + molasses 10.4 + 0.2 17.9 + 0.4 yak + beet molasses + methionine 10.4 + 0.3 18.2 + 0.4 yak + beet molasses + iodinol 10.3 + 0.2 17.8 + 0.6 yak (control) 10.4 + 0.2 13.1 + 0.4

table 2 Assessment of anti-infective activity in experiments on mice A drug the death of mice in days survival 1-3 4-5 % Iodinol four 42.9 According to the recipe 3 3 57.1 Physical solution 5 28.6

Table 3 The influence of the tested compounds on the metabolic processes of calves hypotrophic A drug Hemoglobin, g / l Red blood cells, million / cm ³ Protein, g / l Calcium mmol / L Phosphorus mmol / L By recipe 1 78.24 ± 3.12 85.6 ± 2.36 3.9 ± 0.4 4.4 ± 0.2 61.8 ± 2.4 65.7 ± 2.2 1.56 ± 0.11 2.78 ± 0.15 1.29 ± 0.16 1.62 ± 0.23 According to the recipe 2 79.62 ± 2.75 89.19 ± 2.15 3.9 ± 0.2 4.6 ± 0.1 61.2 ± 2.3 67.3 ± 2.3 1.54 ± 0.19 2.69 ± 0.12 1.31 ± 0.12 1.65 ± 0.18 The control 78.56 ± 2.42 82.8 ± 2.56 3.9 ± 0.2 4.2 ± 0.3 60.8 ± 2.4 62.7 ± 2.6 1.59 ± 0.11 1.67 ± 0.08 1.34 ± 0.17 1.58 ± 0.13 Note: numerator - background indicators;
denominator - indicators for 10 days

Table 4 The influence of the tested compounds on the stimulation of the growth activity of hypotrophic calves Group A drug Body weight (kg) Daily gain (g) At the beginning of the experiment 14 days later one By recipe 1 22.5 ± 2.3 32.4 ± 2.7 720 2 According to the recipe 2 23.1 ± 2.4 33.5 ± 3.1 780 3 The control 24.5 ± 2.3 30.8 ± 2.9 490

Table 5 Clinical efficacy of test formulations for diarrhea A drug Number of calves Clinical recovery achieved in (hour) 24-36 36-48 48-72 Composition 1 12 - 8 four Composition 2 12 6 four 2

Claims (1)

A drug for stimulating metabolic processes, preventing and treating gastrointestinal diseases of calves in the early postnatal period, including succinic acid as an energy stimulator, characterized in that beet molasses are used as a carbohydrate component, methionine is a stimulant of the digestive system, and as an anti-infectious component - iodinol, and water in the following ratio of components based on 0.5 liter:
Succinic acid 5.0 g
Beet molasses 150.0 ml
Methionine 2.0 g
Iodinol 30-50 ml
Water is the rest.