RU2418579C1 - Immunostimulating medication for selenium exchange normalisation and correction of stress states for agricultural animals - Google Patents

Abstract

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention relates to field of veterinary. Medication contains 2,4,6,8-tetramethyl-2,4,6,8-tetraasabicyclo- (3,3,0)- octadiselenon-3,7 as selenium source. As oil, medication contains peach oil with the following component ratio wt %: 2,4,6,8-tetramethyl-2,4,6,8-tetraasabicyclo- (3,3,0)- octadiselenon-3,7 - 20-40, peach oil - the remaining part.

EFFECT: medication possesses high immunostimulating activity, expressed anti-stress effect, is not toxic.

7 tbl, 5 ex

Description

FIELD OF THE INVENTION

The invention relates to veterinary medicine, in particular to preparations for the normalization of selenium metabolism, having an anti-stress effect and an immunostimulating effect for farm animals.

State of the art

Known drug for the treatment and prevention of diseases caused by selenium deficiency in the body of farm animals, containing sodium selenite, barium selenite, aminoacetic acid and paraffin oil, and the components are taken in the following ratio in wt.%:

Sodium Selenite 1-2 Barium selenite 4-7 Aminoacetic acid 2-5 Vaseline oil Up to 100

(see Pat. RU No. 2200013, class A61K 33/04, A61K 31/195, published on October 20, 2002).

The disadvantage of this tool is its high toxicity, a small therapeutic index, and a low effect of increasing the immune status and anti-stress effect.

Known means for the treatment and prevention of diseases caused by selenium deficiency in the body of farm animals and birds. The essence of this invention is the use of bis (benzoylmethyl) selenide as a means for the treatment and prevention of diseases caused by selenium deficiency in the body of farm animals, is used to increase productivity and as a therapeutic and prophylactic agent, with LD ₀ = 15 mg / kg, LD ₅₀ = 71 mg / kg, LD ₁₀₀ = 135 mg / kg (see US Pat. RU No. 2051681, class A61K 33/04, publ. 10.01.1996).

The disadvantage of this tool is the inconvenience of dosage, as well as the insufficiency of immunostimulating and anti-stress effects.

The closest in technical essence and the achieved positive effect and adopted by the authors for the prototype is the drug carcel for the prevention and treatment of obstetric and gynecological diseases in cows, including in wt.%:

Diacetophenonyl selenide 0.15-0.22 Beta carotene 0.1-0.2 Vegetable oil Rest

(see Pat. RU No. 2323719, class A61K 31/00, A61P 31/04, publ. 10.10.2007).

The disadvantage of this drug is the lack of anti-stress effect.

Disclosure of invention

The objective of the invention is to develop a preparation containing selenium in organic form in the form of a selenium-containing analogue of mebicar and having low toxicity, high bioavailability, a pronounced anti-stress effect, and contributing to an increase in the resistance of the organism of farm animals.

The technical result that can be achieved using the present invention is to reduce the toxicity of selenium, increase the bioavailability, anti-stress, immunostimulating and antioxidant effects due to the fact that selenium is in organic form in the form of a selenium-containing analogue of mebicar.

The technical result is achieved using an immunostimulating drug for the normalization of selenium metabolism and the correction of stress conditions for farm animals, including the selenium-containing analogue of mebicar (2,4,6,8-Tetramethyl-2,4,6,8-tetraazabicyclo (3.3.0) octadiselenone-3,7) and peach oil in the following ratio of components in wt.%:

2,4,6,8-tetramethyl-2,4,6,8-tetraazabicyclo (3.3.0) octadiselenone-3.7 20-40 Peach oil rest

The development and implementation of effective measures to combat alimentary and endocrine diseases is a condition for increasing the profitability of animal husbandry, improving the biological value of milk, meat, eggs and other products, as well as reducing the infertility of the breeding stock (see Sidorkin V.A., Klishchenko O.A. / / Veterinary medicine. - 2008. - No. 10. - P.50-51).

Mebicar is a white crystalline powder.

2,4,6,8-tetramethyl-2,4,6,8-tetraazabicyclo (3.3.0) octadione-3.7 (C ₈ H ₁₄ N ₄ O ₂ ).

Mebikar is readily soluble in water and organic solvents, chemically inert and resistant to storage, does not interact with drugs, food components, which allows it to be used regardless of the nature of nutrition and treatment with other drugs (see Karpov AM, Zimakov A.Yu., Makarchikov N .S., Zimakova I.E. Modern problems of psychiatry. - 1994, p.156-158).

In preclinical trials, mebicar did not show teratogenic, mutagenic carcinogenic effects. Side effects are extremely rare; over 20 years of use, isolated cases of dyspeptic disorders and skin itching have been noted. It does not cause addiction, there are no contraindications for use (see Burov Yu.V., Treskov V.G., Drozdov E.S. et al. // New Medicines - Express Inform. - 1983. - No. 11. - C .14-20).

In addition to psychotropic activity, Mebikar has a pronounced hypodynamic effect and a number of pharmacological effects, including nootropic, antioxidant, etc. - both at the level of individual organs, tissues, and the body as a whole (see M. Venediktova, T. Tagirova. New drugs. - Express inform. - 1983. - No. 10. - P.15-16; Zimakova I.E., Gumerov R.Kh., Karpov AM et al. // Terra medica nova. - 2000. - No. 4. - S.30-31).

The introduction of mebicar of 15-20 mg / kg body weight for 5 days a month during the grazing period positively influenced the immune status and dynamics of the body weight of lambs, which was 5.7 kg higher than that of the control (see Daugalieva E. Kh., Filippov VV Immune status and ways of its correction during helminthiases of farm animals. - M.: Agropromizdat, 1991. - 188 p.).

After the introduction of mebicar, a statistically significant increase in the percentage of the population of B-lymphocytes with a C3 receptor was established in the blood of mice (see Mamykova OI // Breeding of agricultural animals for disease resistance and increased resistance in industrial technology. - 1988. - Iss. 8. - P.122).

Studies of many domestic and foreign scientists have proved that selenium is a unique, indispensable and highly effective biologically active element that has a positive effect on the productivity and reproduction rates of animals (see Shakirov Sh., Portnov D., Volkov A. // Dairy and beef cattle breeding. - 2009. - No. 6. - S.18-20).

Considering the mechanism of action as an antioxidant in biological processes, scientists have found that it is part of the enzyme glutathione peroxidase, which, in addition to its other functions, restores PUFAs to prevent further chain reactions of lipid peroxidation, and hydrogen peroxide to prevent the radical from participating hydroxyl (see Shatsky E., Lebedeva I. // Poultry. - 2006. - No. 3. - P.21).

Inorganic compounds of selenium - sodium selenite, barium selenite, etc. are quite toxic (1-2 toxicity class), with low bioavailability (20-30%) and less effective than organic (see Balym Yu.P., Belyaev V.I. ., Ermakova T.I. // Veterinary pathology. - 2007. - No. 2. - P.91-93).

The essence of obtaining an immunostimulating drug to normalize selenium metabolism and correct stress conditions for farm animals is as follows.

To obtain a selenium-containing analogue of mebicar, a solution of 1.98 g (10 mmol) of mebicar in 10 ml of chloroform is poured for 3 minutes into a solution of 3.6 g (30 mmol) of thionyl chloride in 5 ml of chloroform. The solution was stirred for 30 minutes at room temperature, after which 3.75 g (30 mmol) of solid sodium selenide (Na ₂ Se) was added and stirred for 30 minutes. Inorganic salts are filtered off, the solvent is evaporated. The residue is recrystallized from alcohol. Light brown crystals are obtained. Yield 2.2 g (68%), mp. 248-250 ° C.

2,4,6,8-tetramethyl-2,4,6,8-tetraazabicyclo (3.3.0) octadiselenone-3.7 (C ₈ H ₁₄ N ₄ Se ₂ ).

¹ H NMR spectrum (200 MHz; DMSO-d ⁶ ), δ, ppm, J (Hz): 2.39 (12H, s, Me), 3.79 (2H, s, CH). Mass spectrum (EU, 70 eV), m / z (I _rel (%)): 324 [M] ⁺ (100).

The obtained selenium-containing analogue of mebicar - 2,4,6,8-tetramethyl-2,4,6,8-tetraazabicyclo (3.3.0) octadiselenone-3.7 under aseptic conditions is dissolved in sterile peach oil and sealed, while taking the following ratio of components in wt.%:

2,4,6,8-tetramethyl- 2,4,6,8-tetraazabicyclo (3.3.0) octadiselenone-3.7 20-40 Peach oil rest

with subsequent packaging and sterilization.

The implementation of the invention

Examples of specific implementation of the preparation and testing of an immunostimulating drug to normalize selenium metabolism and correct stress conditions for farm animals.

Example 1

An immunostimulating drug to normalize selenium metabolism and correct stress conditions for farm animals is prepared by dissolving 2,4,6,8-tetramethyl-2,4,6,8-tetraazabicyclo (3.3.0) octadiselenone-3.7 under aseptic conditions in peach oil in the following ratio of components in wt.%:

2,4,6,8-tetramethyl- 2,4,6,8-tetraazabicyclo (3.3.0) octadiselenone-3.7 10 Peach oil rest

The resulting preparation, when applied to lambs of two months of age in the analysis of hematological and biochemical blood parameters, showed a relatively small increase in the number of lymphocytes - by 1.21%, erythrocytes - by 0.78% and hemoglobin - by 1.74%, catalase activity - by 3.18 % and glutathione peroxidase - by 4.42%, as well as a relatively small decrease in the concentration of malondialdehyde - by 2.93% and diene conjugates - by 3.62%, the increase in live weight was not so significant and amounted to 1.11% kg per month .

Example 2

Carried out analogously to example 1, but take the following ratio of components in wt.%:

2,4,6,8-tetramethyl- 2,4,6,8-tetraazabicyclo (3.3.0) octadiselenone-3.7 twenty Peach oil rest

The resulting preparation, when applied to lambs of two months of age, increases the number of lymphocytes - by 4.18%, erythrocytes - by 2.26%), hemoglobin - 4.58%), which indicates the stabilization of hematopoiesis, increases the activity of antioxidant enzymes of catalase - by 11.27 % and glutathione peroxidase - by 13.48% and reduces the concentration of malondialdehyde - by 10.37% and diene conjugates - by 9.11% in the blood, and also contributes to an increase in live weight, which amounted to 1.39 kg per month.

Example 3

Carried out analogously to example 1, but take the following ratio of components in wt.%:

2,4,6,8-tetramethyl- 2,4,6,8-tetraazabicyclo (3.3.0) octadiselenone-3.7 thirty Peach oil rest

The preparation obtained when using lambs of two months of age showed that the level of selenium normalized in the blood, and the number of red blood cells increased by 4.16%, lymphocytes by 5.38%, hemoglobin - 4.47%, the activity of catalase increased by 14, 81% and glutathione peroxidase by 19.22%, the concentration of malondialdehyde decreased by 25.35%) and diene conjugates by 18.75%, the increase in live weight was 1.42 kg per month.

Example 4

Carried out analogously to example 1, but take the following ratio of components in wt.%:

2,4,6,8-tetramethyl- 2,4,6,8-tetraazabicyclo (3.3.0) octadiselenone-3.7 40 Peach oil rest

The resulting preparation, when used by lambs of two months of age, showed a pronounced antioxidant effect, consisting in an increase in catalase activity - by 18.29%, glutathione peroxidase - by 24.71% and a decrease in the concentration of malondialdehyde - by 31.42% and diene conjugates by 26.86 % Its introduction was accompanied by an increase in the number of lymphocytes - by 6.72%, erythrocytes - by 5.12% and hemoglobin - by 5.32%, as well as a significant increase in live weight gain, which amounted to 1.64 kg per month.

Example 5

Carried out analogously to example 1, but take the following ratio of components in wt.%:

2,4,6,8-tetramethyl- 2,4,6,8-tetraazabicyclo (3.3.0) octadiselenone-3.7 fifty Peach oil rest

The preparation obtained when using lambs of two months of age showed a less pronounced antioxidant effect than in the previous three examples, and it was expressed in an increase in catalase activity - by 10.38%, glutathione peroxidase - by 12.75% and a decrease in the concentration of malondialdehyde - by 9.84 % and diene conjugates - by 7.87%. The increase in live weight was 1.26 kg per month.

Thus, examples 2, 3 and 4 are the most optimal, since the use of the drug contributed to the greatest increase in live weight gain, selenium concentration, antioxidant enzyme activity and a decrease in the concentration of by-products of lipid peroxidation.

An immunostimulating drug for the normalization of selenium metabolism and the correction of stress conditions for farm animals, made according to example 3 with the following ratio of components in wt.%:

2,4,6,8-tetramethyl- 2,4,6,8-tetraazabicyclo (3.3.0) octadiselenone-3.7 thirty Peach oil rest

was studied for acute toxicity and the effect on the body of rabbits and lambs (Tables 1, 2, 3, 4, 5, 6, 7).

Table 1 Acute toxicity of an immunostimulating drug for the normalization of selenium metabolism and the correction of stress conditions for farm animals, mg / kg per active substance Toxicity parameters Animal species MTD LD ₁₆ LD ₅₀ LD ₈₄ LD ₁₀₀ SLD ₅₀ White rats 97.9 271.6 337.3 458.4 548.1 ± 2.92

To assess the effect of the drug on hematological parameters and determine the approximate pharmacological doses used laboratory white mice, which were divided into four groups (n = 10). The animals of the first group were not administered the drug; they served as a control. Mice of the second, third and fourth groups were injected intramuscularly with the test drug in doses of 2, 6 and 10 mg / kg of live weight, respectively. Three days after drug administration, blood was obtained by decapitation for hematological studies.

When analyzing the data obtained (Tables 2, 3), there is a higher content of red blood cells in the blood of mice that were injected with the drug, compared with the control group. So, in the second group this indicator was higher than in the control by 3.7%, and in the third and fourth - by 8.2 and 6.0%, respectively. The increased production of red blood cells contributes to a synergistic increase in the hemoglobin content with an increase in the dose of the drug, the concentration of which in the third and fourth groups was 10.5 and 3.6% higher than in the control, respectively, and lower in the second group than in the control 1.4%.

table 2 Hematological parameters of white mice with the introduction of an immunostimulating drug to normalize selenium metabolism and correct stress conditions for farm animals (n = 10) Group number Dose mg / kg Red blood cells, 10 ¹² / L Color indicator, units Hemoglobin, g / l White blood cells, 10 ⁹ / L one - 7.81 ± 0.24 1.14 ± 0.05 156.1 ± 6.48 7.34 ± 0.27 2 0.016 8.11 ± 0.32 1.10 ± 0.03 153.8 ± 9.23 7.45 ± 0.29 3 0,032 8.53 ± 0.37 1.19 ± 0.06 174.5 ± 7.12 7.51 ± 0.34 four 0,064 8.36 ± 0.23 1.12 ± 0.05 161.9 ± 8.69 8.53 ± 0.56

The number of leukocytes in all groups was within the physiological norm, and significant differences (not more than 5.3% in both directions) were not observed in the control, second and third groups, and in the fourth group this indicator was higher by 13.9%. Since this increase is not critical and does not go beyond the norm, it can be defined as the activation of the body's defenses in response to an increased dose of the drug.

Table 3 Blood leukogram of white mice after administration of an immunostimulating drug to normalize selenium metabolism and correct stress conditions for farm animals (n = 10),%. Group number Dose mg / kg Basophils Eosinophils Neutrophils Lymphocytes Monocytes M YU P FROM one - - one - - 23.1 ± 4.2 2.21 ± 1.02 68.42 ± 5.5 2.03 ± 0.23 2 0.016 - - - - 22.5 ± 1.8 1.09 ± 0.19 71.2 ± 7.6 2.07 ± 0.41 3 0,032 one - - - 22.1 ± 2.2 2.01 ± 0.73 73.1 ± 6.2 1.98 ± 0.29 four 0,064 - one - one 22.8 ± 6.1 2.07 ± 0.54 72.8 ± 8.1 2.11 ± 1.15

In an experiment to determine the effect of the drug on the productivity and biochemical status of young sheep, three groups of lambs (n = 20) 60 ± 9 days old were used. The lambs of the first group were injected intramuscularly at a dose of 6 mg / kg according to the active substance. The second group did not receive the drug and served as a control. The drug was administered twice with an interval of 45 days. Blood was taken before administration of the drug and after 15 days for two months. Animals were weighed before administration, after 30 days, and at the end of the experiment.

Evaluation of hematological parameters (Table 4) showed that the number of red blood cells in the blood of lambs from the second group increased after 30 days after administration by 7.14%, amounted to 10.22 million / μl, and this value was the highest during the entire experimental period . In general, from the beginning to the end of the experiment in this group, the increase was 12.21%. In the control group, less significant changes occurred, where the number of red blood cells increased by 2.98%.

The hemoglobin level increased gradually in the blood of animals from the experimental group over the course of two months during which the experiment was conducted. So, in the first group, an increase in this indicator was 6.03. In control lambs, the hemoglobin level increased by 0.24%.

The first group showed an increase in the number of leukocytes by 4.33%, respectively, and in the control group - by 2.72%.

Table 4 Hematological parameters and glucose level in the blood of lambs (n = 20) Group number Red blood cells, 10 ¹² / L White blood cells, 10 ⁹ / L Hemoglobin, g / l Color indicator, units Glucose, mmol / L Before drug administration one 9.49 ± 0.51 7.95 ± 0.43 101.15 ± 5.67 0.99 ± 0.07 2.88 ± 0.24 2 9.44 ± 0.54 7.58 ± 0.41 98.98 ± 6.21 0.97 ± 0.07 2.93 ± 0.15 15 days after drug administration one 9.71 ± 0.58 7.99 ± 0.46 102.13 ± 5.72 0.95 ± 0.06 2.92 ± 0.26 2 9.49 ± 0.51 7.60 ± 0.39 99.03 ± 6.29 0.95 ± 0.05 2.84 ± 0.13 30 days after drug administration one 10.22 ± 0.60 8.14 ± 0.56 106.19 ± 6.35 0.91 ± 0.07 3.11 ± 0.29 2 9.47 ± 0.56 7.97 ± 0.45 98.84 ± 6.14 0.94 ± 0.04 2.79 ± 0.18 45 days after drug administration one 10.01 ± 0.55 8.29 ± 0.57 104.92 ± 5.91 0.93 ± 0.06 3.04 ± 0.24 2 9.65 ± 0.59 8.01 ± 0.43 99.37 ± 6.22 0.92 ± 0.05 3.00 ± 0.27 60 days after the first injection one 10.81 ± 0.67 8.31 ± 0.59 107.65 ± 6.04 0.87 ± 0.05 3.19 ± 0.22 2 9.73 ± 0.57 8.04 ± 0.43 99.22 ± 5.78 0.91 ± 0.05 2.97 ± 0.25

As can be seen from the data of table 5, the amount of total protein in the lambs increased, but in the group to which the drug was administered, this increase was more significant than in the control group, in which the increase during the experiment was only 0.73%. In the group of lambs to which the preparation was used, over a similar period of time, this indicator increased by 7.5%.

The glucose level in the control group remained virtually unchanged throughout the experiment, but in the experimental group this indicator increased. In the first group, the increase was 9.72%.

Table 5 The content of total protein and its fractions in the blood serum of lambs (n = 20) Group number Total protein, g / l Protein fractions,% albumin globulins α β γ Before drug administration one 64.18 ± 3.95 38.93 ± 2.29 19.34 ± 1.19 12.54 ± 0.76 27.13 ± 2.07 2 65.11 ± 4.11 39.42 ± 2.12 19.62 ± 1.03 13.35 ± 0.88 26.68 ± 0.76 15 days after drug administration one 65.02 ± 4.25 39.26 ± 2.35 20.71 ± 1.28 12.32 ± 0.81 27.35 ± 1.98 2 65.24 ± 4.16 39.27 ± 2.23 20.46 ± 1.21 13.71 ± 0.91 26.73 ± 0.82 30 days after drug administration one 68.14 ± 4.51 42.89 ± 2.40 17.41 ± 1.42 11.13 ± 0.97 28.69 ± 1.95 2 65.17 ± 4.24 39.76 ± 2.31 19.21 ± 1.30 13.48 ± 0.82 27.12 ± 0.69 45 days after the first injection one 68.77 ± 4.38 42.97 ± 2.61 18.26 ± 1.25 11.53 ± 1.16 28.49 ± 1.91 2 65.54 ± 4.32 40.19 ± 2.37 19.52 ± 1.15 12.76 ± 0.94 26.43 ± 1.03 60 days after the first injection one 69.44 ± 4.57 44.01 ± 2.59 17.15 ± 1.43 10.51 ± 1.21 29.64 ± 2.02 2 65.59 ± 4.29 40.14 ± 2.25 19.17 ± 1.37 12.89 ± 0.99 26.57 ± 1.79

The concentration of diene conjugates over 60 days in the first group decreased by 31.42%, while in the second group, on the contrary, increased by 13.51% (Table 6). The level of malondialdehyde in the control group also increased by 7.24%. In lambs treated with the drug, by 25.80%. This happened because the introduction of selenium-containing drugs led to the activation of antioxidant enzymes. After the use of the drug, there was a significant increase in the activity of glutathione peroxidase. In the group to which the drug was used, this indicator increased by 27.75, and in the control group a decrease of 5.79% was recorded.

15 days after the use of drugs in the experimental groups, the deficiency of selenium was eliminated, the level of which in the first blood samples was 8 times or more lower than the physiological level. So, in lambs, which were administered the drug, by the end of the experiment, the level of selenium increased by 14.1 times from the initial content.

Table 6 Indicators of the system of antioxidant protection of lambs (n = 20) Group Selenium, micromol / l Catalase activity, μm H ₂ O ₂ / l · min · 10 ³ Peroxidase activity, units opt. square / l · sec Glutathione peroxidase activity, μM G-SH / l · min · 10 ³ Glutathione reduced, mmol / l Diene conjugates, units opt. pl. / mg lipid Malondialdehyde, μmol / L Before drug administration one 0.11 ± 0.02 17.94 ± 0.43 38.16 ± 1.79 6.92 ± 0.22 0.51 ± 0.05 0.35 ± 0.03 0.62 ± 0.05 2 0.12 ± 0.04 18.15 ± 0.48 37.89 ± 2.12 6.73 ± 0.19 0.56 ± 0.09 0.32 ± 0.02 0.64 ± 0.05 15 days after drug administration one 1.05 ± 0.05 * 19.58 ± 0.54 * 39.12 ± 1.84 8.21 ± 0.28 * 0.51 ± 0.06 0.29 ± 0.04 0.59 ± 0.05 2 0.11 ± 0.03 18.11 ± 0.46 37.53 ± 2.05 6.76 ± 0.20 0.57 ± 0.07 0.37 ± 0.03 0.67 ± 0.07 30 days after drug administration one 1.06 ± 0.06 * 21.03 ± 0.67 * 41.41 ± 1.86 9.89 ± 0.34 * 0.54 ± 0.06 0.21 ± 0.03 * 0.48 ± 0.06 * 2 0.15 ± 0.04 18.19 ± 0.51 38.37 ± 1.56 6.48 ± 0.23 0.55 ± 0.07 0.35 ± 0.02 0.65 ± 0.05 45 days after administration one 0.81 ± 0.07 * 20.29 ± 0.52 * 40.92 ± 1.73 9.13 ± 0.27 * 0.53 ± 0.03 0.25 ± 0.03 * 0.50 ± 0.04 * 2 0.13 ± 0.04 18.31 ± 0.47 38.24 ± 1.58 6.52 ± 0.19 0.54 ± 0.05 0.38 ± 0.03 0.65 ± 0.05 * 60 days after the first injection one 1.59 ± 0.11 * 20.92 ± 0.59 * 41.46 ± 1.88 9.62 ± 0.31 * 0.54 ± 0.05 0.24 ± 0.04 * 0.46 ± 0.05 * 2 0.14 ± 0.03 18.23 ± 0.41 38.17 ± 1.51 6.34 ± 0.28 0.57 ± 0.06 0.37 ± 0.02 0.69 ± 0.06 * Note: * p <0.05 - the difference is statistically significant between this and the control group

During the observation period, the increase in live weight in lambs treated with the drug was 5.74 kg, and in the control, 4.57 kg (Table 7). In the control group, the total gain was 20.38% less than in the experimental group.

Table 7 The dynamics of the live weight of lambs, kg (n = 20) Group Age months 2 3 four one 11.79 ± 0.76 14.52 ± 0.59 17.53 ± 0.81 2 11.88 ± 0.69 13.89 ± 0.86 16.37 ± 0.74

Thus, it was found that the use of the drug to normalize selenium metabolism and correct stress conditions for farm animals promotes activation of the enzymatic link of the lambs antioxidant defense system, which is expressed in an increase in the activity of catalase, peroxidase, glutathione peroxidase and a decrease in the concentration of malondialdehyde, diene conjugates in the blood. When the drug affects the body, there is an increase in the number of leukocytes, red blood cells and an increase in hemoglobin, normalization of protein and carbohydrate metabolism. Its introduction was accompanied by an increase in the gain in live weight of lambs, which indicates a positive effect on the meat productivity of sheep. Already 15 days after the administration of the drug, the selenium status of animals returned to normal.

The advantages of this drug are that selenium in its composition is taken in the form of a selenium-containing analogue of the mebicar-organo-selenium compound, due to which toxicity is significantly reduced in comparison with inorganic analogues, and the drug has a pronounced immunostimulating and anti-stress effect.

The invention in comparison with the prototype and other technical solutions has the following technical advantages:

- reduction of toxicity;

- increased immunostimulating action;

- pronounced anti-stress effect;

- increase digestibility and bioavailability for the body.

Claims (1)

An immunostimulating drug for normalizing selenium metabolism and correcting stress conditions for farm animals, including an organic source of selenium and oil, characterized in that it contains 2,4,6,8-tetramethyl-2,4,6,8-tetraazabicyclo- as a source of selenium (3,3,0) -octadiselenone-3,7, and as an oil - peach oil in the following ratio of components, wt.%:
2,4,6,8-tetramethyl-2,4,6,8-tetraazabicyclo- (3.3.0) -octadiselenone-3.7 20-40 Peach oil rest