CN101785437B - Chlamys nobilis offspring seed cultivation method - Google Patents
Chlamys nobilis offspring seed cultivation method Download PDFInfo
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- CN101785437B CN101785437B CN2009101943350A CN200910194335A CN101785437B CN 101785437 B CN101785437 B CN 101785437B CN 2009101943350 A CN2009101943350 A CN 2009101943350A CN 200910194335 A CN200910194335 A CN 200910194335A CN 101785437 B CN101785437 B CN 101785437B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention discloses a Chlamys nobilis offspring seed cultivation method. Offspring seeds are pretreated by water and are performed with parent Chlamys nobilis selection, induction for oviposition and artificial insemination to cultivate Chlamys nobilis offspring seed larva. The invention adjusts water quality by processing water for the offspring seed before inputting larva, thus effectively improving larva survival rate and distortion ratio, and lowering production cost.
Description
Technical field
The present invention relates to a kind of Chlamys nobilis offspring seed cultivation method.
Background technology
Chlamys nobilis Chlamys nobilis natural distribution is in Honshu, four countries and the nine divisions of China in remote antiquity of Japan, Indonesia and South China Sea.Chlamys nobilis is nutritious, and delicious meat is at one of main mariculture economic shellfish in China south.In the Chlamys nobilis seedling raising process, substantially all adopt hydrostatic cultivation and flowing water to change water law and cultivate seed.Hydrostatic cultivation concrete operations quantity of exchanged water every day is 1/3 of an original water volume; It is the simulation sea environment that flowing water changes water law, is the circulation system that seedling water causes a low speed, and specific practice is that certain distance is provided with shower nozzle around nursery pond, makes current (Jin Qizeng, 1996 of seedling water running from the seawater of shower nozzle ejection; Du Tao etc., 2004; Lu Yihua etc., 2004).
In the seedling raising process, change the water basic goal and be to improve the seedling water environment and keep water quality pure and fresh.Also there is obvious defects yet change water, mainly is embodied in and changes the living environment that water has changed the young, make the young all be in a kind of stress situation every day, be unfavorable for young growth.In addition, changing water in a large number can also bring the harmful organisms of external source and increase production cost to seedling water.
Summary of the invention
The object of the invention provides a kind of Chlamys nobilis offspring seed cultivation method, utilizes water for larval nursing preliminary treatment, close shellfish selection, induced spawning, artificial insemination and larval culture.This invention can obviously improve larva survive ratio and distortion ratio, reduces production costs.
The present invention includes following processing step:
(1) water for larval nursing preliminary treatment: water for larval nursing is handled through sand filtration earlier, the thickness 40-60cm of sand filter blanket fine sand; The water of sand filtration is injected nursery pond; Use 0.3-0.6g/m
3Strong chlorine oil carries out water body disinfection, and disinfecting time is 12-15h, neutralizes with sodium thiosulfate again; The seawater aeration 12-15h that disinfected.
(2) selection of close shellfish and accelerating: the parent who grows seedlings can be from cultured population, and parent's quantity is at least 100, and gonad development is to physiological maturity more than 1 age age for the parent shellfish.
(3) induced spawning: close shellfish passes through stimulation by running water, dries in the shade and intensification water stimulation back discharging gamete.Close shellfish is placed in plastic basin or the Plastic Drum, carries out stimulation by running water, stimulation time is about 20-30min; The close shellfish that crosses through stimulation by running water is placed on does not have sunlit place to dry in the shade, and the time is about 20-30min; Close shellfish after drying in the shade is placed on the basin of laying eggs, and adds intensification water (temperature improves 3-4 ℃) induced spawning, through close shellfish discharging gamete behind the 20-40min.
(4) artificial insemination: induce the ovum that is produced to filter close shellfish, put into Plastic Drum, add seawater (volume be plastic basin 2/3) simultaneously, add the mixing sperm of a little then, and fully stir and carry out artificial insemination with 300 mesh sieve thin,tough silk.After fertilization 1h carries out washing the first time ovum, unnecessary sperm in the flush away seawater.2h after the artificial insemination changes fertilized egg over to nursery pond and carries out larval culture.
(5) larval culture: density is 1-2/mL, the chrysophyceae twice of throwing something and feeding every day, chrysophyceae concentration 3000-5000cell/mL; Water temperature 26-29 ℃, salinity 30 ‰, inflation keeps the water body dissolved oxygen to be no less than 5.0mg/L.
The present invention can obviously improve larva survive ratio and distortion ratio, has reduced production cost.
Embodiment
In April, 2007, the sand filtration seawater is injected 4 4m * 4m * 1.8m nursery pond, depth of water 1.5m respectively; Each pond adds 12g strong chlorine oil sterilization 12h, neutralizes with sodium thiosulfate again; The seawater aeration 12h that disinfected.Select from the expensive chlamys farreri cultured population of Zhanjiang crow onyx that 120 gonad maturities are individual to be the parent, male and female quantity is respectively 80 and 40, shellfish 1.5 ages of age.The parent through flowing water, dry in the shade and intensification seawater (temperature improve 3-4 ℃) stimulates back discharging gamete.Female and male gametophyte is filtered with 300 mesh sieve thin,tough silk, put into the 30L Plastic Drum respectively, add the 20L seawater simultaneously, in the bucket that fills oogamete, add a spot of mixing sperm then, fully stir evenly and carry out artificial insemination.Insemination back 1h carries out washing the first time ovum.Insemination back 2h changes fertilized egg over to 4 nursery ponds respectively and cultivates, 2/mL of density.Between larval stage, the chrysophyceae twice of throwing something and feeding every day, chrysophyceae concentration is about 5000cell/mL.Eyespot appears in insemination back 12-15 days, can throw in adherance.After the young adheres to, throw something and feed chrysophyceae and flat algae twice, chrysophyceae and flat algae concentration all are about 5000cell/mL.Water temperature 28-29 ℃, salinity 30 ‰, inflation keeps water body dissolved oxygen 5.0mg/L.Each nursery pond is emerged ratio (commercial seedling/fertilized egg) for 5-10%, and is about 3% without the water for larval nursing processed group ratio of emerging.
Claims (2)
1. a Chlamys nobilis offspring seed cultivation method is characterized in that comprising the steps:
(1) water for larval nursing preliminary treatment: water for larval nursing is handled through sand filtration earlier, the thickness 40-60cm of sand filter blanket fine sand; The water of sand filtration is injected nursery pond; Use 0.3-0.6g/m
3Strong chlorine oil carries out water body disinfection, and disinfecting time is 12-15h, neutralizes with sodium thiosulfate again; The seawater aeration 12-15h that disinfected;
(2) selection of close shellfish and accelerating: the parent who grows seedlings can be from cultured population, and parent's quantity is at least 100, and shellfish age of parent, gonad development was to the maturing stage more than 1 age;
(3) induced spawning: close shellfish through stimulation by running water, dry in the shade and and intensification water stimulate back discharging gamete; Close shellfish is placed in plastic basin or the Plastic Drum, carries out stimulation by running water, stimulation time is 20-30min; The close shellfish that crosses through stimulation by running water is placed on does not have sunlit place to dry in the shade, and the time is 20-30min; Close shellfish after drying in the shade is placed on the basin of laying eggs, and adds temperature and improves 3-4 ℃ water induced spawning, through close shellfish discharging gamete behind the 20-40min;
(4) artificial insemination: induce the ovum that is produced to filter close shellfish with 300 mesh sieve thin,tough silk, put into Plastic Drum, add volume simultaneously and be 2/3 seawater of plastic basin, add a little then and mix sperm, and fully stir and carry out artificial insemination, after fertilization 1h, carry out washing the first time ovum, unnecessary sperm in the flush away seawater, 2h after the artificial insemination changes fertilized egg over to nursery pond and carries out larval culture;
(5) larval culture: density is 1-2/ml, the chrysophyceae twice of throwing something and feeding every day, chrysophyceae concentration 3000-5000cell/ml; Water temperature 28-29 ℃, salinity 30 ‰, inflation keeps the water body dissolved oxygen to be no less than 5.0mg/l.
2. according to claims 1 described a kind of Chlamys nobilis offspring seed cultivation method, it is characterized in that not carrying out the water body exchange in the pelagic period.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2009101943350A CN101785437B (en) | 2009-11-24 | 2009-11-24 | Chlamys nobilis offspring seed cultivation method |
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| CN2009101943350A CN101785437B (en) | 2009-11-24 | 2009-11-24 | Chlamys nobilis offspring seed cultivation method |
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| CN101785437A CN101785437A (en) | 2010-07-28 |
| CN101785437B true CN101785437B (en) | 2011-12-07 |
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| CN2009101943350A Expired - Fee Related CN101785437B (en) | 2009-11-24 | 2009-11-24 | Chlamys nobilis offspring seed cultivation method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103168734A (en) * | 2013-04-15 | 2013-06-26 | 蓬莱市天保海水养殖有限公司 | Parent patinopecten yessoensis culturing and hatching method |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102124967A (en) * | 2010-12-30 | 2011-07-20 | 山东海益宝水产股份有限公司 | Method for inducing Atrina pectinata Linnaeus to quickly spawn |
| CN103229739B (en) * | 2013-05-17 | 2014-06-11 | 琼海时达渔业有限公司 | Artificial induced spawning method of tridacna shell |
| CN103583439A (en) * | 2013-11-20 | 2014-02-19 | 文登市水产技术推广站 | Method for improving egg washing efficiency of bay scallops |
| CN106035166B (en) * | 2016-06-20 | 2019-03-08 | 中国科学院南海海洋研究所 | It is a kind of effectively to avoid the autogamous physical method of giant clam shellfish |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1340297A (en) * | 2000-08-25 | 2002-03-20 | 中国水产科学研究院黄海水产研究所 | Method for producing triploid of young Jiekong scallop |
| CN1739343A (en) * | 2004-08-24 | 2006-03-01 | 中国科学院海洋研究所 | A kind of tetraploid preparation method who is applicable to chlamys farreri |
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2009
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1340297A (en) * | 2000-08-25 | 2002-03-20 | 中国水产科学研究院黄海水产研究所 | Method for producing triploid of young Jiekong scallop |
| CN1739343A (en) * | 2004-08-24 | 2006-03-01 | 中国科学院海洋研究所 | A kind of tetraploid preparation method who is applicable to chlamys farreri |
Non-Patent Citations (3)
| Title |
|---|
| 宋志乐.《栉孔扇贝海上中间培育阶段提高保苗率的试验研究》.《海洋科学》.1986,第10卷(第4期),31－36. * |
| 李文姬.《虾夷扇贝生产性育苗的高产技术》.《水产科学》.1997,第16卷(第2期),21-23. * |
| 金启增.《华贵栉孔扇贝生产性育苗高产试验》.《热带海洋》.1991,第10卷(第3期),8-15. * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103168734A (en) * | 2013-04-15 | 2013-06-26 | 蓬莱市天保海水养殖有限公司 | Parent patinopecten yessoensis culturing and hatching method |
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| CN101785437A (en) | 2010-07-28 |
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