CN103109758A - Tilapia fry rearing method - Google Patents
Tilapia fry rearing method Download PDFInfo
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- CN103109758A CN103109758A CN2012105401841A CN201210540184A CN103109758A CN 103109758 A CN103109758 A CN 103109758A CN 2012105401841 A CN2012105401841 A CN 2012105401841A CN 201210540184 A CN201210540184 A CN 201210540184A CN 103109758 A CN103109758 A CN 103109758A
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Abstract
The invention discloses a tilapia fry rearing method. The tilapia fry rearing method comprises the following steps: breeding parent tilapia fries which are bigger than nine-sieve, picking parent tilapias which are disease free, injury free, strong and good in sexual maturity to rear, hybridizing the female parent tilapias and with the male parent tilapias according to ratio of stocking, picking up roes, incubating the roes with flowing incubating water in an incubating tank, cultivating the roes by marking in bold, and cultivating fries. Oviposition period is shortened by about seven days, incubating rate is as high as 67.9%, fry survival rate is 89.6%, each female tilapia lays 6700 fries each year averagely, fry production rate is improved by 30%, and the sizes of the fries are uniform. By means of the tilapia fry rearing method, fries can be reared all through a year, a large amount of tilapia fries are reared and the tilapia fries grow fast and are strong in stress resistance. The tilapia fries reared in mass are born at the same time, gonad developments are synchronous, sizes are the same, characters are unified, and heredity purity is high. Moreover, the tilapia fry rearing method has the advantages that incubating period is short, incubating efficiency is high, inheritable characters are good, fertility is strong, and fries can be reared in an overwintering mode.
Description
Technical field
The present invention relates to fish production of hybrid seeds seedling growing process field, particularly relate to a kind of Tilapia mossambica production of hybrid seeds seedling-cultivating method.
Background technology
Tilapia mossambica (Tilapia) is commonly called as: African crucian, belong to and planting: Perciformes, Percoidei, eel Gyrinocheilidae, Tilapia mossambica belong to a kind of medium and small shape fish.In current international culture fishery, Tilapia mossambica is the cultured freshwater fish that various countries' emphasis scientific research is cultivated, and is described as one of main source of following animal protein.Tilapia mossambica originates in Africa, belongs to the tropic fishes of Family Cichlidae, usually moves in fresh water, also can move in the salt water of different salt content, can survive in the lake river, in the shallow water in pond, have very strong adaptive capacity, and have extremely strong adaptability in the less water of dissolved oxygen.
Edible fishes along with world's self-sow, fall sharply as resources such as cods, make and fish for quota and significantly reduce, cause the fish products prices such as global cod sharply to rise, the development of the taste of Tilapia mossambica and the consumption market of aquatic products matches, and is becoming new substitute, and is more and more being accepted by market, home and abroad, day by day be subject to people's approval, its price is occupied the forefront in popular cultured fishes.
The Tilapia mossambica early sexual maturity, oviposition period is short, and juvenile fish is hatched in the oral cavity, and Reproduction Conditions is less demanding, natural propagation in large water area impounded body.The Tilapia mossambica of self-sow namely reached sexual maturity in 6 months, weigh the raun about 200 grams, fish brood amount is many 1000～1500 left and right, between breeding period, milter extruding belly penetrates white seminal fluid, and raun output mature egg also is contained in the oral cavity with it at once, makes ovum fertilization, fertilized egg is grown in the raun oral cavity, can hatch juvenile fish in 4～5 days during 25～30 ℃ of water temperatures.Juvenile fish leaves parent when yolk sac disappears and have certain ability.But it is longer that the juvenile fish time is hatched in the oral cavity, and incubation rate and survival rate are all lower, and Parental Germplasms is easily degenerated, and cause fertility to descend, and fry kind specification kind is irregular, and will be difficult to hatching when water temperature lower (lower than 20 ℃).Therefore, for the demand of Tilapia mossambica aquaculture development, need to carry out artificial breeding.
Yet, along with developing rapidly of Tilapia mossambica aquaculture, also increasing to the demand of Tilapia mossambica breeding.Reached more than 80 ten thousand mu as China Guangdong Province the whole province cultivating tilapia area, it is nearly 50% that cultured output accounts for national Tilapia mossambica gross yield, and Tilapia mossambica breeding fry annual requirement surpasses 1,000,000,000 tails, but present high-quality seed is supplied but less than 600,000,000 tails, and seriously supply falls short of demand.Therefore strengthen the Tilapia mossambica fine-variety breeding and the factory fry technical research has vast potential for future development.
Chinese invention patent application (application number: 200810220791.3) disclose " artificial seed's seedling-cultivating method of a kind of Tilapia mossambica ", its technical scheme is: by parent population seed selection and cultivation, hasten parturition and get ovum, artificial incubation and fry rearing and complete the method for producing the Tilapia mossambica fry, reach and improve fry quality and survival rate, and be suitable for suitability for industrialized production.
But, still existing Parental Germplasms in prior art and degenerate, fertility descends, the seed size kind is irregular, emergence rate is low, is difficult to provide rapidly seedling supply in enormous quantities after opening the winter, and the problem such as fast, the parent of stabilization characteristics of genetics of seed selection growth and the regulating and controlling water quality of growing seedlings how.
Therefore, for existing problems of the prior art, needing badly provides that a kind of brooding time is short, emergence rate is high, genetic character is good, fertility is strong, and the Tilapia mossambica production of hybrid seeds seedling growing process of growing seedlings of can surviving the winter seems particularly important.
Summary of the invention
The object of the invention is to avoid weak point of the prior art and provide that a kind of brooding time is short, hatching efficiency is high, genetic character is good, fertility is strong, and the Tilapia mossambica production of hybrid seeds seedling-cultivating method that can survive the winter and grow seedlings.
Purpose of the present invention is achieved through the following technical solutions:
A kind of Tilapia mossambica production of hybrid seeds seedling-cultivating method is provided, includes following steps:
Step 1, the seed selection of parent's fish fry: then the Selection parent fish fry moves to culturing pool and cultivates to being greater than or equal to 50g, sorting raun, milter cultivating the pond thick cultivation of mark to being greater than or equal to 9 towards sieve;
Step 2, parent fish rearing:
⑴ parent fish pond water filling after sterilizing 3, and be that 60～80 purpose bolting silks filter with the aperture, the water filling depth of water is retained to 80～100cm and carries out drug disinfection, the parent fish pond water temperature stability during at 18～25 ℃ respectively minute pond put the raun that sub-elects, milter in a suitable place to breed to cultivate parent population;
⑵ throw in the parent population feed of protein content 20～45%, and feeding volume is 2%; (the parent population feed can be expanded pellet diet);
⑶ changed water 30% by changing water and throwing in the quicklime regulating water quality every 15～25 days, every applying quick lime on the 30th once, the amount of discharging is 10～15g quicklime/m
3Water body;
Step 3, seed selection: select anosisly without wound, physique is healthy and strong, and the good parent population of maturity is as kind of a fish, and female parent population is greater than or equal to 400g, and male parent population is greater than or equal to 1000g;
Step 4, breeding: the female parent population that will select and male parent population are to put to the mating pond in 25: 10～30: 10 or the breeding of mating net cage by the ratio of stocking;
Step 5, get ovum: keeping water temperature is 25～28 ℃, collects fertilized egg after 1～3 day;
Step 6, hatching:
Fertilized egg is cleaned fish scale, foreign material with 5 ‰ salt solution, after rinsing 2～3 times, put into the hatching cylinder and carry out Lotic hatching by Spawning water;
Described Spawning water is washed ovum simultaneously in the Lotic hatching process, daily requirement adds water, and to need to change water, quantity of exchanged water when dense be each 15%～25% when water quality is dark;
Keep described Spawning water flow and flow velocity, make fertilized egg be in the state that evenly seethes under flow action, and keep the Spawning water in the hatching cylinder to overflow; (can make Spawning water spilling water amount remain on 2.5 l/min);
Controlling described Spawning water water source keeps between 28～31 ℃ of water temperatures;
Collect and just to hatch fry to supporting temporarily basin, support temporarily when disappearing to yolk, thick cultivation is marked in immigration fry rearing pond; (generally can support temporarily 1);
Step 7, mark are slightly cultivated:
Fry being moved into fry rearing pond continuation cultivate, is 1～2 ℃ with the water temperature temperature difference of supporting temporarily basin during immigration;
Move into before prerequisite fry rearing pond sterilization, water 30% was changed in then entry after 100 mesh sieve thin,tough silk filter in every 10 days in cultivating process;
After the fry membrane the 2nd～3 day or move into after the protein content that began in 2nd to throw something and feed be 40～50% eel powder or Tilapia mossambica juvenile fish powder, water mixes bulk and repeatedly throws something and feeds on a small quantity, the overall control of throwing something and feeding is in 8～12% of fry body weight; (as: can throw something and feed every day 11 times, between every day 8:00～18:00, per hour 1 time, throw something and feed overall control the fry body weight 10%);
Step 8, emerge: cultivate after 15～21 days, can emerge when the fry body grows to 2～3cm, stop expecting half a day before emerging, salvage and put into the fishpond after emerging and carry out the marketable fish cultivation or sell.
Wherein, in described step 1, described parent's fish fry is through the parent's fish fry after group breeding method and family selective breeding method screening.
Wherein, in described step 7, when fry rearing is pool cultivated, water quality environment is controlled to be: 22～28 ℃ of water temperatures, ph value 7.2～7.9, ammonia-nitrogen content less than 0.5mg/L, content of nitrite less than 0.15mg/L, dissolved oxygen content greater than 5.0mg/L.
Wherein, in described step 2, described raun, milter minute pond breeding density ratio of putting in a suitable place to breed respectively are 22: 10～25:10.
Wherein, in described step 2,
Before described putting in a suitable place to breed, the raun of selecting, milter are by 3～5% common salt aqueous solution dipping bath 5～10min, perhaps concentration is liquor potassic permanganate dipping bath 20～30min at 20 ℃ of temperature of 20mg/l, and perhaps the 30mg/L Betagen Solution dipping bath 5min of 1% available iodine carries out disinfection;
Before described putting in a suitable place to breed, when the water filling depth of water is retained to 80～100cm, every mu discharge urea 1.5～2kg, phosphate fertilizer 4～5kg carries out drug disinfection, makes water transparence remain on 28～32cm.
Wherein, in described step 6, the hatching cylinder soaks 30min with 30～50mg/L liquor potassic permanganate before use.
Wherein, the Spawning water of described step 6 is cycling use of water, and it keeps the processing method of water quality to be:
Filter and ultraviolet disinfection with sandstone and 200 mesh sieve thin,tough silk;
It is 28～30 ℃ that heated for controlling temperature keeps the water source water temperature;
Add salt or sea crystal and keep salinity 5 ‰;
When adding water or changing water, keep ph value 7.2～7.9, ammonia-nitrogen content is less than 0.5mg/L, and content of nitrite is less than 0.15mg/L, and dissolved oxygen content is greater than 5.0mg/L;
Perhaps add the EDTA complexation heavy metal ion.
Wherein, in the hatching cylinder of described step 6, each the hatching cylinder put into 900ml～1100 ml fertilized egg, correspond to 90,000～130,000 fertilized eggs.
Wherein, in described step 6, the fry that hatches of collecting in a time period in each hatching basin is supported in basin, by every basin 1.0～20,000 tails to temporary.Grow synchronously with the fry of guaranteeing each time period, specification is consistent.
Wherein, the fry rearing pond is set to cage type fry rearing pond, and described cage type fry rearing pond breeding density is every case 20,000 tails;
Perhaps the fry rearing pond is set to cement pit structure fry rearing pond, and described cement pit structure fry rearing pond breeding density is every pond 6～80,000 tails.
Beneficial effect of the present invention:
A kind of Tilapia mossambica production of hybrid seeds seedling-cultivating method of the present invention includes following steps:
Step 1, the seed selection of parent's fish fry: the Selection parent fish fry is being cultivated the pond thick cultivation of mark to being greater than or equal to 9 towards sieve, then move to culturing pool cultivate to be greater than or equal to 50g(as: cultivate to 80g), sorting raun, milter.
Step 2, parent fish rearing:
⑴ parent fish pond water filling after sterilizing 3, and be that 60～80 purpose bolting silks filter with the aperture, the water filling depth of water is retained to 80～100cm and carries out drug disinfection, the parent fish pond water temperature stability during at 18～25 ℃ respectively minute pond put the raun that sub-elects, milter in a suitable place to breed to cultivate parent population.
Parent fish pond can adopt dry method sterilization, every mu of applying quick lime 50～60kg, dissolve rear immediately while hot full pond evenly splash; Beginning water filling after 3 days, is 70 purpose silk cover filterings with the aperture, is filled to depth of water 90cm.
Before putting in a suitable place to breed, parent population can carry out drug disinfection.
Pond water temperature can be stabilized in 18 ℃.
⑵ throw in the parent population feed of protein content 20～45%, and feeding volume is 2%;
The parent population feed can be expanded pellet diet, and the feeding volume of throwing in the expanded pellet diet of protein content 33% is 2%.
⑶ changed water 30% by changing water and throwing in the quicklime regulating water quality every 15～25 days, every applying quick lime on the 30th once, the amount of discharging is 10～15g quicklime/m
3Water body.
Can change water 30% every about 20 days, every 30 days can applying quick lime once, the amount of discharging can be 15g/m
3
Step 3, seed selection: select anosisly without wound, physique is healthy and strong, and the good parent population of maturity is as kind of a fish, and female parent population is greater than or equal to 400g, and male parent population is greater than or equal to 1000g;
Step 4, breeding: the female parent population that will select and male parent population are to put to the mating pond in 25: 10～30: 10 or the breeding of mating net cage by the ratio of stocking;
Step 5, get ovum: keeping water temperature is 25～28 ℃, collects fertilized egg after 1～3 day;
Step 6, hatching:
Fertilized egg is cleaned fish scale, foreign material with 5 ‰ salt solution, after rinsing 2～3 times, put into the hatching cylinder and carry out Lotic hatching by Spawning water;
Spawning water is washed ovum simultaneously in the Lotic hatching process, daily requirement adds water, and to need to change water, quantity of exchanged water when dense be each 15%～25% when water quality is dark;
Keep described Spawning water flow and flow velocity, make fertilized egg be in the state that evenly seethes under flow action, and keep the Spawning water in the hatching cylinder to overflow; (can make Spawning water spilling water amount remain on 2.5 l/min);
Controlling described Spawning water water source keeps between 28～31 ℃ of water temperatures;
Collect and just to hatch fry to supporting temporarily basin, support temporarily when disappearing to yolk, thick cultivation is marked in immigration fry rearing pond; (generally can support temporarily 1);
Step 7, mark are slightly cultivated:
Fry being moved into fry rearing pond continuation cultivate, is 1～2 ℃ with the water temperature temperature difference of supporting temporarily basin during immigration;
Move into before prerequisite fry rearing pond sterilization, water 30% was changed in then entry after 100 mesh sieve thin,tough silk filter in every 10 days in cultivating process;
After the fry membrane the 2nd～3 day or move into after the protein content that began in 2nd to throw something and feed be 40～50% eel powder or Tilapia mossambica juvenile fish powder, water mixes bulk and repeatedly throws something and feeds on a small quantity, the overall control of throwing something and feeding is in 8～12% of fry body weight; (as: can throw something and feed every day 11 times, between every day 8:00～18:00, per hour 1 time, throw something and feed overall control the fry body weight 10%);
Step 8, emerge: cultivate after 15～21 days, can emerge when the fry body grows to 2～3cm, stop expecting half a day before emerging, salvage and put into the fishpond after emerging and carry out the marketable fish cultivation or sell.
The present invention compared to existing technology, the Gunther parent fish spawning cycle shortened about 7 days, incubation rate reaches 67.9%, weed survival rate 89.6%, average every tail raun is produced seedling 6700 tails per year, produce the seedling amount and improved 30%, and seed size is neat.And owing to adopting the production of growing seedlings of workshop industrialization pattern, can survive the winter and grow seedlings, thereby make it possible to grow seedlings the whole year.Simultaneously can also carry out Tilapia mossambica parent's seed selection by group breeding method and family selective breeding method, growth is fast except having in seed selection, the Tilapia mossambica parent of individual large, strong stress resistance, stabilization characteristics of genetics, select the different female male parent of family in source to carry out cross match, prevent that effectively inbreeding from mixing, can breed that growth in enormous quantities is fast, the Tilapia mossambica seed of strong stress resistance, and property brings out birth simultaneously, gonad development is synchronous, specification is consistent, the fry that proterties is unified, genetic purity is high in batches.Adopt and manually get ovum, reduced the nutrient consumption when raun incubates fry, accelerate the speed that female fish ovaries is grown again, effectively shorten the ovum cycle, lay eggs number of times and the number of emerging of every tail raun have been improved, and isolated harmful organisms, pathogene and purification water for larval nursing, effectively improved fry hatching rate and cultivating survival rate.
Embodiment
With the following Examples the present invention is described in further detail.
Embodiment 1
One of embodiment of a kind of Tilapia mossambica production of hybrid seeds seedling-cultivating method of the present invention includes following steps:
Step 1, the seed selection of parent's fish fry: then the Selection parent fish fry moves to culturing pool and cultivates to being greater than or equal to 50g, sorting raun, milter cultivating the pond thick cultivation of mark to being greater than or equal to 9 towards sieve.
Concrete, can cultivate to 80g sorting raun, milter.
Step 2, parent fish rearing:
⑴ parent fish pond water filling after sterilizing 3, and be that 60～80 purpose bolting silks filter with the aperture, the water filling depth of water is retained to 80～100cm and carries out drug disinfection, the parent fish pond water temperature stability during at 18～25 ℃ respectively minute pond put the raun that sub-elects, milter in a suitable place to breed to cultivate parent population.
Concrete, parent fish pond can adopt the dry method sterilization, every mu of applying quick lime 50～60kg, dissolve rear immediately while hot full pond evenly splash; Beginning water filling after 3 days, is 70 purpose silk cover filterings with the aperture, is filled to depth of water 90cm.
Concrete, drug disinfection can be carried out before putting the raun that sub-elects, milter in a suitable place to breed in minute pond respectively.Can pass through 5% common salt aqueous solution dipping bath 10min.
Concrete, pond water temperature can be stabilized in 18 ℃.
⑵ throw in the parent population feed of protein content 20～45%, and feeding volume is 2%;
Concrete, the parent population feed can be expanded pellet diet, the feeding volume of throwing in the expanded pellet diet of protein content 33% is 2%.
Concrete, the breeding density of raun, milter is than being 25:10.
Concrete, urea 2kg can every mu be discharged in the parent fish rearing pond, and phosphate fertilizer 5kg controls water transparence and remains on 31cm.
Concrete, parent fish rearing pond water can keep that 28 ℃ of temperature, ph value are 7.6, ammonia-nitrogen content less than below 0.5mg/L, content of nitrite less than 0.15mg/L, dissolved oxygen content greater than 5.0mg/L.
⑶ changed water 30% by changing water and throwing in the quicklime regulating water quality every 15～25 days, every applying quick lime on the 30th once, the amount of discharging is 10～15g quicklime/m
3Water body.
Concrete, can change water 30% every about 20 days, every 30 days can applying quick lime once, the amount of discharging can be 10g/m
3
Step 3, seed selection: select anosisly without wound, physique is healthy and strong, and the good parent population of maturity is as kind of a fish, and female parent population is greater than or equal to 400g, and male parent population is greater than or equal to 1000g.
Concrete, raun can be selected weight 600 grams, and milter can be selected weight 1100 grams.
Being specially raun belly anal fin the place ahead has anus, gonopore and uropoiesis hole, and ripe individual gonopore is outstanding, belly expands, the well-balanced softness of front and back size, ventrimeson are recessed; Milter belly anal fin the place ahead only has anus and cloacal aperture, and ripe individual cloacal aperture is given prominence to greatly, and sexual gland is lax and ruddy, presses fish body belly to have milky seminal fluid to flow out with have gentle hands, and entry is namely loose.
Step 4, breeding: the female parent population that will select and male parent population are to put to the mating pond in 25: 10～30: 10 or the breeding of mating net cage by the ratio of stocking.
Concrete, the ratio of stocking of female parent population and male parent population is can 30: 10.
Step 5, get ovum: keeping water temperature is 25～28 ℃, collects fertilized egg after 1～3 day.
Concrete, can keep water temperature is 28 ℃, collects fertilized egg after 3 days.
Step 6, hatching:
Fertilized egg is cleaned fish scale, foreign material with 5 ‰ salt solution, after rinsing 2～3 times, put into the hatching cylinder and carry out Lotic hatching by Spawning water.
Spawning water is washed ovum simultaneously in the Lotic hatching process, daily requirement adds water, and to need to change water, quantity of exchanged water when dense be each 15%～25% when water quality is dark.
Concrete, the hatching cylinder can soak 30min with the 50mg/L liquor potassic permanganate before use, then fertilized egg is put into the hatching cylinder and hatches.
Concrete, fertilized egg can be cleaned the foreign material such as fish scale with 5 ‰ salt solution, after rinsing 3 times, then put into and hatch cylinder and carry out the Spawning water Lotic hatching.
Concrete, hatch cylinder and can put into the fertilized egg of 900ml～1100, correspond to 90,000～130,000 fertilized eggs.
Keep described Spawning water flow and flow velocity, make fertilized egg be in the state that evenly seethes under flow action, and keep the Spawning water in the hatching cylinder to overflow.Can make Spawning water spilling water amount remain on 2.5L/min.
Concrete, hatch and wash ovum and can all add water every day, need to change water when water quality is dark when dense, quantity of exchanged water can be for change 20% left and right at every turn.
Concrete, Spawning water can filter with sandstone and 200 mesh sieve thin,tough silk, and can sterilize by ultraviolet ray.
Controlling described Spawning water water source keeps between 28～31 ℃ of water temperatures.Can control water temperature is 31 ℃.
Concrete, control 30 ℃ of hatching water temperatures, salinity 5 ‰, ph value 7.2～7.9, ammonia-nitrogen content is less than 0.5mg/L, and content of nitrite is less than 0.15mg/L, and dissolved oxygen content is greater than 5.0mg/L.
Perhaps, can also add the EDTA complexation heavy metal ion.
Collect and just to hatch fry to supporting temporarily basin, support temporarily when disappearing to yolk, thick cultivation is marked in immigration fry rearing pond.
Generally can support temporarily 1.
Concrete, time period collects fry in each hatching basin and is placed on and supports temporarily in basin, every basin 1.5 ten thousand tails, and the fry of guaranteeing each time period grows synchronous, and specification is consistent.
Step 7, mark are slightly cultivated:
Fry being moved into fry rearing pond continuation cultivate, is 1～2 ℃ with the water temperature temperature difference of supporting temporarily basin during immigration.
Concrete, can be 2 ℃ with the water temperature temperature difference of supporting temporarily basin during immigration.
Move into before prerequisite fry rearing pond sterilization, water 30% was changed in then entry after 100 mesh sieve thin,tough silk filter in every 10 days in cultivating process;
Concrete, fry rearing pond water temperature can be 28 ℃, and the ph value is 7.9, and ammonia-nitrogen content is less than 0.5mg/L, and content of nitrite is less than 0.15mg/L, and dissolved oxygen content is greater than 5.0mg/L, changes water 30% in every 10 days.
After the fry membrane the 2nd～3 day or move into after the protein content that began in 2nd to throw something and feed be 40～50% eel powder or Tilapia mossambica juvenile fish powder, water mixes bulk and repeatedly throws something and feeds on a small quantity, the overall control of throwing something and feeding is in 8～12% of fry body weight;
Concrete, can throw something and feed every day 11 times, between every day 8:00～18:00, per hour 1 time, the overall control of throwing something and feeding is in 10% of fry body weight.
Concrete, the fry rearing pond is set to cage type fry rearing pond, and cage type fry rearing pond specification can be 3m * 3m * 1m, and breeding density is every case 20,000 tails.
Perhaps the fry rearing pond is set to cement structures fry rearing pond, and cement structures fry rearing pond specification can be 4m * 6m * 1m, and breeding density is every pond 80,000 tails.
Step 8, emerge: cultivate after 15～21 days, can emerge when the fry body grows to 2～3cm, stop expecting half a day before emerging, salvage and put into the fishpond after emerging and carry out the marketable fish cultivation or sell.
Concrete, cultivate after 21 days, when growing to 3cm, the fry body can emerge.
Embodiment 2
One of embodiment of a kind of Tilapia mossambica production of hybrid seeds seedling-cultivating method of the present invention includes following steps:
Step 1, the seed selection of parent's fish fry: the Selection parent fish fry slightly is cultivated to 10 towards sieve cultivating the pond mark, then moves to the culturing pool cultivation to being greater than or equal to 50～100g, sorting raun, milter.
Step 2, parent fish rearing:
⑴ parent fish pond is sterilized through dry method, every mu of applying quick lime 50～60kg, after dissolving immediately while hot full pond evenly splash, water filling after 3 days, and be that 80 purpose bolting silks filter with the aperture, the water filling depth of water is retained to 100cm and carries out drug disinfection, the parent fish pond water temperature stability more than 18 ℃ the time respectively minute pond put the raun that sub-elects, milter in a suitable place to breed to cultivate parent population;
⑵ throw in the expanded parent population feed of protein content 35%, and feeding volume is 2%.
⑶ changed water 30% by changing water and throwing in the quicklime regulating water quality every 20 days, every applying quick lime on the 30th once, the amount of discharging is 15g quicklime/m
3Water body.
Concrete, the breeding density of raun, milter is than being 25:10.
Concrete, before putting in a suitable place to breed, the raun of selecting, milter carry out disinfection by the 30mg/L Betagen Solution dipping bath 5min of 1% available iodine.
Concrete, before putting in a suitable place to breed, when the water filling depth of water is retained to 100cm, every mu discharge urea 2kg, phosphate fertilizer 5kg carries out drug disinfection, makes water transparence remain on 32cm.
Concrete, during the parent fish pond cultivation, water quality environment is controlled to be: 28 ℃ of water temperatures, ph value 7.9, ammonia-nitrogen content less than 0.5mg/L, content of nitrite less than 0.15mg/L, dissolved oxygen content greater than 5.0mg/L.
Step 3, seed selection: select anosisly without wound, physique is healthy and strong, and the good parent population of maturity is as kind of a fish, and female parent population weight is 600g, and male parent population weight is 1200g.
Step 4, breeding: the female parent population that will select and male parent population are to put to mating pond at 30: 10 or the breeding of mating net cage by the ratio of stocking.
Step 5, get ovum: keeping water temperature is 28 ℃, collects fertilized egg after 3 days.
Step 6, hatching:
Fertilized egg is cleaned fish scale, foreign material with 5 ‰ salt solution, after rinsing 3 times, put into the hatching cylinder and carry out Lotic hatching by Spawning water.
Concrete, the hatching cylinder can soak 30min with the 50mg/L liquor potassic permanganate before use.
Spawning water is washed ovum simultaneously in the Lotic hatching process, daily requirement adds water, and to need to change water, quantity of exchanged water when dense be each 20% left and right when water quality is dark.
Keep Spawning water flow and flow velocity, make fertilized egg be in the state that evenly seethes under flow action, and keep the Spawning water in the hatching cylinder to overflow.
Concrete, can make Spawning water spilling water amount remain on 2.5L/min.
Concrete, Spawning water can filter and ultraviolet disinfection with sandstone and 200 mesh sieve thin,tough silk.
Concrete, it is 30 ℃ that heated for controlling temperature keeps Spawning water water source water temperature.
Concrete, add salt or sea crystal and keep salinity 5 ‰;
When adding water or changing water, keep ph value 7.9, ammonia-nitrogen content is less than 0.5mg/L, and content of nitrite is less than 0.15mg/L, and dissolved oxygen content is greater than 5.0mg/L;
In case of necessity, can also add the EDTA complexation heavy metal ion.
Concrete, each hatching cylinder can put into 1100ml fertilized egg, correspond to 90,000～130,000 fertilized eggs.
Concrete, the fry that hatches that can collect in a time period in each hatching basin is supported in basin, by every basin 1.0～20,000 tails to temporary.Grow synchronously with the fry of guaranteeing each time period, specification is consistent.
Collect and just to hatch fry to supporting temporarily basin, control 31 ℃ of water temperatures, supported 1 temporarily, support temporarily when disappearing to yolk, thick cultivation is marked in immigration fry rearing pond.
Step 7, mark are slightly cultivated:
Fry being moved into fry rearing pond continuation cultivate, is 2 ℃ with the water temperature temperature difference of supporting temporarily basin during immigration.
Move into before prerequisite fry rearing pond sterilization, water 30% was changed in then entry after 100 mesh sieve thin,tough silk filter in every 10 days in cultivating process.
Concrete, fry rearing pond water temperature is 28 ℃, and the ph value is 7.9, and ammonia-nitrogen content is less than 0.5mg/L, and content of nitrite is less than 0.15mg/L, and dissolved oxygen content is greater than 5.0mg/L, changes water 30% in every 10 days.
After the fry membrane the 3rd day or move into after the protein content that began in 2nd to throw something and feed be 40～50% eel powder or Tilapia mossambica juvenile fish powder, water mixes bulk and repeatedly throws something and feeds on a small quantity, the overall control of throwing something and feeding is in 10% of fry body weight.
Concrete, can throw something and feed every day 11 times, between every day 8:00～18:00, per hour 1 time, 10% left and right of overall control in the fry body weight of throwing something and feeding.
Concrete, the fry rearing pond can be set to cage type fry rearing pond, and cage type fry rearing pond specification is 3m * 3m * 1m, and breeding density is every case 20,000 tails.
Perhaps, the fry rearing pond can be set to cement pit structure fry rearing pond, and cement pit structure fry rearing pond specification is 4m * 6m * 1m, and breeding density is every pond 6～80,000 tails.
Step 8, emerge: cultivate after 15～21 days, can emerge when the fry body grows to 2～3cm, stop expecting half a day before emerging, salvage and put into the fishpond after emerging and carry out the marketable fish cultivation or sell.
Embodiment 3
One of embodiment of a kind of Tilapia mossambica production of hybrid seeds seedling-cultivating method of the present invention includes following steps:
Step 1, the seed selection of parent's fish fry: the Selection parent fish fry is slightly cultivated to 9 towards sieve cultivating the pond mark, then moves to culturing pool and cultivates to 50g, sorting raun, milter.
Step 2, parent fish rearing:
⑴ parent fish pond is sterilized through dry method, every mu of applying quick lime 50～60kg, after dissolving immediately while hot full pond evenly splash, water filling after 3 days, and be that 60 purpose bolting silks filter with the aperture, the water filling depth of water is retained to 80cm and carries out drug disinfection, the parent fish pond water temperature stability more than 18 ℃ the time respectively minute pond put the raun that sub-elects, milter in a suitable place to breed to cultivate parent population;
⑵ throw in the expanded parent population feed of protein content 30%, and feeding volume is 2%.
⑶ changed water 30% by changing water and throwing in the quicklime regulating water quality every 20 days, every applying quick lime on the 30th once, the amount of discharging is 15g quicklime/m
3Water body.
Concrete, the breeding density of raun, milter is than being 22:10.
Concrete, before putting in a suitable place to breed, the raun of selecting, milter carry out disinfection by 3% common salt aqueous solution dipping bath 5min.
Concrete, before putting in a suitable place to breed, when the water filling depth of water is retained to 80cm, every mu discharge urea 1.5kg, phosphate fertilizer 4kg carries out drug disinfection, makes water transparence remain on 28cm.
Concrete, during the parent fish pond cultivation, water quality environment is controlled to be: 22 ℃ of water temperatures, ph value 7.2, ammonia-nitrogen content less than 0.5mg/L, content of nitrite less than 0.15mg/L, dissolved oxygen content greater than 5.0mg/L.
Step 3, seed selection: select anosisly without wound, physique is healthy and strong, and the good parent population of maturity is as kind of a fish, and female parent population weight is 400g, and male parent population weight is 1000g.
Step 4, breeding: the female parent population that will select and male parent population are to put to the mating pond in 25: 10～30: 10 or the breeding of mating net cage by the ratio of stocking.
Step 5, get ovum: keeping water temperature is 25 ℃, collects fertilized egg after 1～3 day.
Step 6, hatching:
Fertilized egg is cleaned fish scale, foreign material with 5 ‰ salt solution, after rinsing 2 times, put into the hatching cylinder and carry out Lotic hatching by Spawning water.
Concrete, the hatching cylinder can soak 30min with the 30mg/L liquor potassic permanganate before use.
Spawning water is washed ovum simultaneously in the Lotic hatching process, daily requirement adds water, and to need to change water, quantity of exchanged water when dense be each 20% left and right when water quality is dark.
Keep Spawning water flow and flow velocity, make fertilized egg be in the state that evenly seethes under flow action, and keep the Spawning water in the hatching cylinder to overflow.
Concrete, can make Spawning water spilling water amount remain on 2.5L/min.
Concrete, Spawning water can filter and ultraviolet disinfection with sandstone and 200 mesh sieve thin,tough silk.
Concrete, it is 28 ℃ that heated for controlling temperature keeps Spawning water water source water temperature.
Concrete, add salt or sea crystal and keep salinity 5 ‰.
When adding water or changing water, keep ph value 7.2, ammonia-nitrogen content is less than 0.5mg/L, and content of nitrite is less than 0.15mg/L, and dissolved oxygen content is greater than 5.0mg/L;
In case of necessity, can also add the EDTA complexation heavy metal ion.
Concrete, each hatching cylinder can put into 900 ml fertilized egg, correspond to 90,000 fertilized eggs
Concrete, the fry that hatches that can collect in a time period in each hatching basin is supported in basin, by every basin 1.0 ten thousand tails to temporary.Grow synchronously with the fry of guaranteeing each time period, specification is consistent.
Collect and just to hatch fry to supporting temporarily basin, control 29 ℃ of water temperatures, supported 1 temporarily, support temporarily when disappearing to yolk, thick cultivation is marked in immigration fry rearing pond.
Step 7, mark are slightly cultivated:
Fry being moved into fry rearing pond continuation cultivate, is 1 ℃ with the water temperature temperature difference of supporting temporarily basin during immigration.
Move into before prerequisite fry rearing pond sterilization, water 30% was changed in then entry after 100 mesh sieve thin,tough silk filter in every 10 days in cultivating process.
Concrete, fry rearing pond water temperature is 22 ℃, and the ph value is 7.2, and ammonia-nitrogen content is less than 0.5mg/L, and content of nitrite is less than 0.15mg/L, and dissolved oxygen content is greater than 5.0mg/L, changes water 30% in every 10 days.
After the fry membrane the 2nd day or move into after the protein content that began in 2nd to throw something and feed be 40～50% eel powder or Tilapia mossambica juvenile fish powder, water mixes bulk and repeatedly throws something and feeds on a small quantity, the overall control of throwing something and feeding is in 10% of fry body weight.
Concrete, can throw something and feed every day 11 times, between every day 8:00～18:00, per hour 1 time, 10% left and right of overall control in the fry body weight of throwing something and feeding.
Concrete, the fry rearing pond can be set to cage type fry rearing pond, and cage type fry rearing pond specification is 3m * 3m * 1m, and breeding density is every case 20,000 tails.
Perhaps, the fry rearing pond can be set to cement pit structure fry rearing pond, and cement pit structure fry rearing pond specification is 4m * 6m * 1m, and breeding density is every pond 6～80,000 tails.
Step 8, emerge: cultivate after 15 days, can emerge when the fry body grows to 2cm, stop expecting half a day before emerging, salvage and put into the fishpond after emerging and carry out the marketable fish cultivation or sell.
Embodiment 4
A kind of Tilapia mossambica production of hybrid seeds seedling-cultivating method of the present invention includes following steps:
Step 1, the seed selection of parent's fish fry: the Selection parent fish fry is slightly cultivated to 9 towards sieve cultivating the pond mark, then moves to culturing pool and cultivates to 90g, sorting raun, milter.
Step 2, parent fish rearing:
⑴ parent fish pond is sterilized through dry method, every mu of applying quick lime 50～60kg, after dissolving immediately while hot full pond evenly splash, water filling after 3 days, and be that 75 purpose bolting silks filter with the aperture, the water filling depth of water is retained to 95cm and carries out drug disinfection, the parent fish pond water temperature stability more than 18 ℃ the time respectively minute pond put the raun that sub-elects, milter in a suitable place to breed to cultivate parent population.
⑵ throw in the expanded parent population feed of protein content 33%, and feeding volume is 2%.
⑶ changed water 30% by changing water and throwing in the quicklime regulating water quality every 20 days, every applying quick lime on the 30th once, the amount of discharging is 15g quicklime/m
3Water body.
Concrete, the breeding density of raun, milter is than being 24:10.
Concrete, before putting in a suitable place to breed, the raun of selecting, milter carry out disinfection by the 30mg/L Betagen Solution dipping bath 5min of 1% available iodine.
Concrete, before putting in a suitable place to breed, when the water filling depth of water is retained to 95cm, every mu discharge urea 1.8kg, phosphate fertilizer 4.5kg carries out drug disinfection, makes water transparence remain on 30cm.
Concrete, during the parent fish pond cultivation, water quality environment is controlled to be: 27 ℃ of water temperatures, ph value 7.5, ammonia-nitrogen content less than 0.5mg/L, content of nitrite less than 0.15mg/L, dissolved oxygen content greater than 5.0mg/L.
Step 3, seed selection: select anosisly without wound, physique is healthy and strong, and the good parent population of maturity is as kind of a fish, and female parent population weight is 500g, and male parent population weight is 11000g.
Step 4, breeding: the female parent population that will select and male parent population are to put to mating pond at 25: 10 or the breeding of mating net cage by the ratio of stocking.
Step 5, get ovum: keeping water temperature is 27 ℃, collects fertilized egg after 2 days.
Step 6, hatching:
Fertilized egg is cleaned fish scale, foreign material with 5 ‰ salt solution, after rinsing 3 times, put into the hatching cylinder and carry out Lotic hatching by Spawning water.
Concrete, the hatching cylinder can soak 30min with the 40mg/L liquor potassic permanganate before use.
Spawning water is washed ovum simultaneously in the Lotic hatching process, daily requirement adds water, and to need to change water, quantity of exchanged water when dense be each 20% left and right when water quality is dark.
Keep Spawning water flow and flow velocity, make fertilized egg be in the state that evenly seethes under flow action, and keep the Spawning water in the hatching cylinder to overflow.
Concrete, can make Spawning water spilling water amount remain on the 2.5L/min left and right.
Concrete, Spawning water can filter and ultraviolet disinfection with sandstone and 200 mesh sieve thin,tough silk.
Concrete, it is 29 ℃ that heated for controlling temperature keeps Spawning water water source water temperature.
Concrete, add salt or sea crystal and keep salinity 5 ‰.
When adding water or changing water, keeping the ph value is 7.4, and ammonia-nitrogen content is less than 0.5mg/L, and content of nitrite is less than 0.15mg/L, and dissolved oxygen content is greater than 5.0mg/L;
In case of necessity, can also add the EDTA complexation heavy metal ion.
Concrete, each hatching cylinder can put into 1100 ml fertilized egg, correspond to 90,000～130,000 fertilized eggs.
Concrete, the fry that hatches that can collect in a time period in each hatching basin is supported in basin, by every basin 1.6 ten thousand tails to temporary.Grow synchronously with the fry of guaranteeing each time period, specification is consistent.Can obtain the fry that proterties is unified, genetic purity is high thus.
Collect and just to hatch fry to supporting temporarily basin, control 29～31 ℃ of water temperatures, supported 1 temporarily, support temporarily when disappearing to yolk, thick cultivation is marked in immigration fry rearing pond.
Step 7, mark are slightly cultivated:
Fry being moved into fry rearing pond continuation cultivate, is 1.5 ℃ with the water temperature temperature difference of supporting temporarily basin during immigration.
Move into before prerequisite fry rearing pond sterilization, water 30% was changed in then entry after 100 mesh sieve thin,tough silk filter in every 10 days in cultivating process.
Concrete, fry rearing pond water temperature is 27 ℃, and the ph value is 7.6, and ammonia-nitrogen content is less than 0.5mg/L, and content of nitrite is less than 0.15mg/L, and dissolved oxygen content is greater than 5.0mg/L, changes water 30% in every 10 days.
After the fry membrane the 3rd day or move into after the protein content that began in 2nd to throw something and feed be 40～50% eel powder or Tilapia mossambica juvenile fish powder, water mixes bulk and repeatedly throws something and feeds on a small quantity, the overall control of throwing something and feeding is in 10% of fry body weight.
Concrete, can throw something and feed every day 11 times, between every day 8:00～18:00, per hour 1 time, 10% left and right of overall control in the fry body weight of throwing something and feeding.
Concrete, the fry rearing pond can be set to cage type fry rearing pond, and cage type fry rearing pond specification is 3m * 3m * 1m, and breeding density is every case 20,000 tails.
Perhaps, the fry rearing pond can be set to cement pit structure fry rearing pond, and cement pit structure fry rearing pond specification is 4m * 6m * 1m, and breeding density is every pond 6～80,000 tails.
Step 8, emerge: cultivate after 20 days, can emerge when the fry body grows to 2.5cm, stop expecting half a day before emerging, salvage and put into the fishpond after emerging and carry out the marketable fish cultivation or sell.
The fry method of the Tilapia mossambica of Tilapia mossambica production of hybrid seeds seedling-cultivating method of the present invention in compared to existing technology, the Gunther parent fish spawning cycle shortened about 7 days, incubation rate can reach 67.9%, weed survival rate average out to 89.6%, average every tail raun is produced seedling 6700 tails per year, produce the seedling amount and improved 30%, and seed size is neat.And owing to adopting the production of growing seedlings of workshop industrialization pattern, can survive the winter and grow seedlings, thereby make it possible to grow seedlings the whole year.
The present invention carries out the postsearch screening seed selection by colony's seed selection and family selective breeding method to Tilapia mossambica parent fish fry, growth is fast except having in seed selection, the Tilapia mossambica parent of individual large, strong stress resistance, stabilization characteristics of genetics, select the different female male parent of family in source to carry out cross match, prevent that effectively inbreeding from mixing, can breed that growth in enormous quantities is fast, the Tilapia mossambica seed of strong stress resistance, and property brings out birth simultaneously, gonad development is synchronous, specification is consistent, the fry that proterties is unified, genetic purity is high in batches.Adopt and manually get ovum, reduced the nutrient consumption when raun incubates fry, accelerate the speed that female fish ovaries is grown again, effectively shorten the ovum cycle, lay eggs number of times and the number of emerging of every tail raun have been improved, and isolated harmful organisms, pathogene and purification water for larval nursing, effectively improved fry hatching rate and cultivating survival rate.。
Should be noted that at last; above embodiment is only in order to illustrate technical scheme of the present invention but not limiting the scope of the invention; although with reference to preferred embodiment, the present invention has been done detailed description; those of ordinary skill in the art is to be understood that; can modify or be equal to replacement technical scheme of the present invention, and not break away from essence and the scope of technical solution of the present invention.
Claims (10)
1. Tilapia mossambica production of hybrid seeds seedling-cultivating method is characterized in that: include following steps:
Step 1, the seed selection of parent's fish fry: then the Selection parent fish fry moves to culturing pool and cultivates to being greater than or equal to 50g, sorting raun, milter cultivating the pond thick cultivation of mark to being greater than or equal to 9 towards sieve;
Step 2, parent fish rearing:
⑴ parent fish pond water filling after sterilizing 3, and be that 60～80 purpose bolting silks filter with the aperture, the water filling depth of water is retained to 80～100cm and carries out drug disinfection, the parent fish pond water temperature stability during at 18～25 ℃ respectively minute pond put the raun that sub-elects, milter in a suitable place to breed to cultivate parent population;
⑵ throw in the parent population feed of protein content 20～45%, and feeding volume is 2%;
⑶ changed water 30% by changing water and throwing in the quicklime regulating water quality every 15～25 days, every applying quick lime on the 30th once, the amount of discharging is 10～15g quicklime/m
3Water body;
Step 3, seed selection: select anosisly without wound, physique is healthy and strong, and the good parent population of maturity is as kind of a fish, and female parent population is greater than or equal to 400g, and male parent population is greater than or equal to 1000g;
Step 4, breeding: the female parent population that will select and male parent population are to put to the mating pond in 25: 10～30: 10 or the breeding of mating net cage by the ratio of stocking;
Step 5, get ovum: keeping water temperature is 25～28 ℃, collects fertilized egg after 1～3 day;
Step 6, hatching:
Fertilized egg is cleaned fish scale, foreign material with 5 ‰ salt solution, after rinsing 2～3 times, put into the hatching cylinder and carry out Lotic hatching by Spawning water;
Described Spawning water is washed ovum simultaneously in the Lotic hatching process, daily requirement adds water, and to need to change water, quantity of exchanged water when dense be each 15%～25% when water quality is dark;
Keep described Spawning water flow and flow velocity, make fertilized egg be in the state that evenly seethes under flow action, and keep the Spawning water in the hatching cylinder to overflow;
Controlling described Spawning water water source keeps between 28～31 ℃ of water temperatures;
Collect and just to hatch fry to supporting temporarily basin, support temporarily when disappearing to yolk, thick cultivation is marked in immigration fry rearing pond;
Step 7, mark are slightly cultivated:
Fry being moved into fry rearing pond continuation cultivate, is 1～2 ℃ with the water temperature temperature difference of supporting temporarily basin during immigration;
Move into before prerequisite fry rearing pond sterilization, water 30% was changed in then entry after 100 mesh sieve thin,tough silk filter in every 10 days in cultivating process;
After the fry membrane the 2nd～3 day or move into after the protein content that began in 2nd to throw something and feed be 40～50% eel powder or Tilapia mossambica juvenile fish powder, water mixes bulk and repeatedly throws something and feeds on a small quantity, the overall control of throwing something and feeding is in 8～12% of fry body weight;
Step 8, emerge: cultivate after 15～21 days, can emerge when the fry body grows to 2～3cm, stop expecting half a day before emerging, salvage and put into the fishpond after emerging and carry out the marketable fish cultivation or sell.
2. a kind of Tilapia mossambica production of hybrid seeds seedling-cultivating method according to claim 1 is characterized in that: in described step 1, described parent's fish fry is through the parent's fish fry after group breeding method and family selective breeding method screening.
3. a kind of Tilapia mossambica production of hybrid seeds seedling-cultivating method according to claim 1, it is characterized in that: in described step 7, when fry rearing is pool cultivated, water quality environment is controlled to be: 22～28 ℃ of water temperatures, ph value 7.2～7.9, ammonia-nitrogen content less than 0.5mg/L, content of nitrite less than 0.15mg/L, dissolved oxygen content greater than 5.0mg/L.
4. a kind of Tilapia mossambica production of hybrid seeds seedling-cultivating method according to claim 1 is characterized in that: in described step 2, described raun, milter minute pond breeding density ratio of putting in a suitable place to breed respectively are 22: 10～25:10.
5. a kind of Tilapia mossambica production of hybrid seeds seedling-cultivating method according to claim 1 is characterized in that: in described step 2,
Before described putting in a suitable place to breed, the raun of selecting, milter are by 3～5% common salt aqueous solution dipping bath 5～10min, perhaps concentration is liquor potassic permanganate dipping bath 20～30min at 20 ℃ of temperature of 20mg/l, and perhaps the 30mg/L Betagen Solution dipping bath 5min of 1% available iodine carries out disinfection;
Before described putting in a suitable place to breed, when the water filling depth of water is retained to 80～100cm, every mu discharge urea 1.5～2kg, phosphate fertilizer 4～5kg carries out drug disinfection, makes water transparence remain on 28～32cm.
6. a kind of Tilapia mossambica production of hybrid seeds seedling-cultivating method according to claim 1, is characterized in that: in described step 6, hatch cylinder and soak 30 min with 30～50mg/L liquor potassic permanganate before use.
7. a kind of Tilapia mossambica production of hybrid seeds seedling-cultivating method according to claim 6, it is characterized in that: the Spawning water of described step 6 is cycling use of water, it keeps the processing method of water quality to be:
Filter and ultraviolet disinfection with sandstone and 200 mesh sieve thin,tough silk;
It is 28～30 ℃ that heated for controlling temperature keeps the water source water temperature;
Add salt or sea crystal and keep salinity 5 ‰;
When adding water or changing water, keep ph value 7.2～7.9, ammonia-nitrogen content is less than 0.5mg/L, and content of nitrite is less than 0.15mg/L, and dissolved oxygen content is greater than 5.0mg/L;
Perhaps add the EDTA complexation heavy metal ion.
8. a kind of Tilapia mossambica production of hybrid seeds seedling-cultivating method according to claim 1 is characterized in that: in the hatching cylinder of described step 6, each hatching cylinder put into 900ml～1100 ml fertilized egg, correspond to 90,000～130,000 fertilized eggs.
9. a kind of Tilapia mossambica production of hybrid seeds seedling-cultivating method according to claim 1, is characterized in that: in described step 6, collect hatching in the extremely temporarily foster basin of fry, by every basin 1.0～20,000 tails in each hatching basin in a time period.
10. a kind of Tilapia mossambica production of hybrid seeds seedling-cultivating method according to claim 1, it is characterized in that: the fry rearing pond is set to cage type fry rearing pond, described cage type fry rearing pond breeding density is every case 20,000 tails, perhaps the fry rearing pond is set to cement pit structure fry rearing pond, and described cement pit structure fry rearing pond breeding density is every pond 6～80,000 tails.
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| CN103947581A (en) * | 2014-03-31 | 2014-07-30 | 中国科学院南海海洋研究所 | Method for improving synchronous mating of seahorses |
| CN104756902A (en) * | 2015-01-23 | 2015-07-08 | 杨国华 | Method for cultivating Tilapia |
| CN104756909A (en) * | 2015-03-27 | 2015-07-08 | 广西壮族自治区水产科学研究院 | Standard culture device and method for tilapia mossambica breeding family fries |
| CN106614130B (en) * | 2016-11-30 | 2019-08-27 | 英山县名德鱼苗孵化养殖有限公司 | A kind of early numerous fast breeding method of Tilapia mossambica |
| CN106614130A (en) * | 2016-11-30 | 2017-05-10 | 英山县名德鱼苗孵化养殖有限公司 | Early propagation and quick cultivation method of tilapia |
| CN107155962A (en) * | 2017-05-15 | 2017-09-15 | 何庆堃 | The pond breeding method of male Tilipia |
| CN107771715A (en) * | 2017-10-27 | 2018-03-09 | 芜湖新华联文化旅游开发有限公司 | It is used for the propagation method of kind madai viewed and admired in a kind of aquarium exhibition cylinder |
| CN108719144A (en) * | 2018-04-27 | 2018-11-02 | 安徽省农业科学院水产研究所 | A kind of family selective breeding method of red line strain Mandarin fish |
| CN109006652A (en) * | 2018-09-29 | 2018-12-18 | 广西壮族自治区水产科学研究院 | A kind of Tilapia mossambica production of hybrid seeds seedling system and method based on Internet of Things |
| CN112655600A (en) * | 2019-12-06 | 2021-04-16 | 海南勤富实业有限公司 | Method for quickly cultivating tilapia mossambica in early stage |
| CN113016670A (en) * | 2021-03-30 | 2021-06-25 | 海南省海洋与渔业科学院 | Tilapia mossambica saline water culture method |
| CN113016670B (en) * | 2021-03-30 | 2023-02-03 | 海南省海洋与渔业科学院 | Tilapia mossambica saline water culture method |
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