CN106035166B - It is a kind of effectively to avoid the autogamous physical method of giant clam shellfish - Google Patents
It is a kind of effectively to avoid the autogamous physical method of giant clam shellfish Download PDFInfo
- Publication number
- CN106035166B CN106035166B CN201610454759.6A CN201610454759A CN106035166B CN 106035166 B CN106035166 B CN 106035166B CN 201610454759 A CN201610454759 A CN 201610454759A CN 106035166 B CN106035166 B CN 106035166B
- Authority
- CN
- China
- Prior art keywords
- ovum
- giant clam
- seawater
- bucket
- filling
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Farming Of Fish And Shellfish (AREA)
Abstract
The autogamous physical method of giant clam shellfish is effectively avoided the invention discloses a kind of.They, are placed in the spermiation bucket for filling normal temperature seawater by all giant clams distribution ID numbers hastened parturition respectively, after spermiation 2-3 minute, remove giant clam and with 500 mesh silk cover filtering tissue blocks, and filtrate is sperm, mark sperm number "
Description
Technical field
The invention belongs to the breeding of giant clam shellfish, ecological resources in marine fishery industry to restore field, and in particular to Yi Zhongyou
Effect avoids the autogamous physical method of giant clam shellfish.
Background technique:
Giant clam shellfish, is a kind of important marine economic shellfish, meat it is fresh and sweet it is crisp and refreshing, have been reported that have nourishment for vitality effect, together
When, shell can Carving Machining at valuable craftwork.Once there were a large amount of distributions at China South Sea, there is data to suggest that, China
The types such as Tridacna (chamestrachea) maxima (R ding), sarranine giant clam, squama giant clam, Che oyster are mainly distributed in the South Sea.Formula giant clam in library has closely disappeared in China at present,
Its giant clam type also faces illegal mining and steals the unfavorable situations such as digging and variation of ecology and environment, the current world (Washington, DC pact) rule
The wild individual of serial giant clam type is determined and has sold type to forbid adopting, library formula giant clam is classified as animals under first-class state protection, phosphorus by China
Giant clam is classified as second class protection animal.Therefore carry out the artificial breeding technology of giant clam shellfish for restoring China South Sea resource, protect
South Sea ecological environment has a very important significance.
Giant clam is kind of a hermaphroditic shellfish, usual protandry, and when Spawning discharges sperm first, finally discharges ovum
Son, it is intermediate to be mingled with smart ovum mixture release toward contact.The appearance that a large amount of self progenies how are effectively avoided when artificial breeding, for
The industrialized development of giant clam artificial breeding and the protection of germ plasm resource have a very important significance.Aquatic products is saved with the method for low temperature
Animal sperm has had lot of documents report.But sperm fertilization vigor is inhibited using low temperature, and passes through physical filtering method
Remove sperm and obtain unfertilized ovum, so that the smart ovum from same individual be avoided be fertilized, be giant clam shellfish artificial seed cultivation with
And it prevents germplasm degeneration from providing technical support and has not been reported.
Summary of the invention:
The autogamous physical method of giant clam shellfish is effectively avoided the object of the present invention is to provide a kind of.
Of the invention effectively avoids the autogamous physical method of giant clam shellfish, which is characterized in that all giant clams hastened parturition point
With ID number, they are placed in the spermiation bucket for filling normal temperature seawater respectively, after spermiation 2-3 minutes, remove giant clam simultaneously
Tissue block is filtered with 500 mesh sieve tulles, filtrate is sperm, is marked sperm number " ♂+ID number "；The giant clam shellfish of removal is set simultaneously
In filling intermediate bucket 1 minute of medium temperature seawater, then takes out and be placed in the ovum for filling low temperature seawater discharge bucket, discharged in ovum
It allows giant clam to continue discharge ovum 15-20 minutes in bucket, after discharge, giant clam is taken out, obtain ovum liquid, while with 300 meshes
Tulle filters ovum liquid, and ovum is located in bolting silk net, recovery of ova, and record ovum is marked as " ♀+ID number ", the ovum that will be collected into
It is respectively placed in the bucket for filling normal temperature seawater, restores in case fertilization according to label；
Gamete inferior is abandoned after checking smart oogamete quality, the different sperm in source and ovum are subjected to people as needed accordingly
Work is by precision processing.
It is preferred that the seawater is all sand filtration seawater, described take out giant clam is that giant clam taking-up medium temperature seawater is anti-
Multiple cleaning, is subsequently placed in renewal cultivation pond.
It is preferred that the seawater that the normal temperature seawater is 28 DEG C or so, the seawater that the medium temperature seawater is 24 DEG C or so, institute
The seawater that the low temperature seawater stated is 20 DEG C or so.
The present invention inhibits sperm fertilization vigor using low temperature, and by physical filtering method remove sperm obtain not by
Smart ovum cultivates and prevents germplasm degeneration from mentioning for giant clam shellfish artificial seed so that the smart ovum from same individual be avoided to be fertilized
For technical support.
Specific embodiment:
The following examples are further illustrations of the invention, rather than limiting the invention.
1 Tridacna (chamestrachea) maxima (R ding) Tridacna maxima of embodiment is autogamous to be avoided and takes precautions against:
At the beginning of 2016, we have carried out Tridacna (chamestrachea) maxima (R ding) (Tridacna in Chinese Academy of Sciences's Sanya, Hainan compbined test station
Maxima) artificial seedling rearing's technical research, induced spawning method are hastened parturition kind of shellfish 2, to avoid self-fertilization, improve seed breeding
Efficiency concrete operations are as follows:
1, prepare clean sand filter seawater in advance, and be made normal temperature seawater (28 DEG C or so), medium temperature (transition) seawater (24 DEG C),
Low temperature seawater (20 DEG C) each 30L is spare.
2, sperm is collected under normal temperature condition.It distributes all giant clams hastened parturition to ID number, they is placed in room temperature sperm respectively
Bucket (carry out semen collection) is discharged, after serotonin injection, starts within 5 minutes spermiation occur, when spermiation is about 2-3
After minute, removes giant clam and filter tissue block and other sundries in sperm with 500 mesh sieve tulles, filtrate is sperm, and is marked
Remember " ♂+ID number ".Giant clam is placed in intermediate bucket (ID is corresponding) 1 minute for filling medium temperature seawater simultaneously, then takes out and places it in
Fill the ovum discharge bucket of low temperature seawater (ID number is corresponding).
3, recovery of ova under cryogenic conditions.Giant clam is allowed to continue discharge sexual gland in the ovum discharge bucket for filling low temperature seawater mixed
It closes product (ovum) 15-20 minutes, after discharge is basic, obtains ovum liquid, giant clam taking-up is cleaned repeatedly with medium temperature seawater, so
It is placed on renewal cultivation pond, while filtering ovum liquid with 300 mesh sieve tulles, ovum is located in bolting silk net, recovery of ova, records ovum
Marked as " ♀+ID number ".
4, the room temperature of ovum restores.The ovum being collected into is respectively placed in the bucket for filling normal temperature seawater according to label, it is extensive
Multiple 5 minutes in case fertilization, ovum are identified as " ♀+ID "
5, microexamination essence oogamete quality abandons gamete inferior, accordingly as needed by the different sperm in source and ovum
Artificial insemination processing is carried out at normal temperature.
By fully controllable mode in the present embodiment, heterologous insemination obtains Che oyster fertilization rate reached to 90%, hatching rate
85% or more ideal expected results.
Embodiment the autogamous of oyster Hippopus hippopus 2: Che avoids and takes precautions against:
At the beginning of 2016,200L volume plastic barrel 4, the artificial breeding work of Che oyster (Hippopus hippopus) is carried out
Make.To avoid self-fertilization, raising seed breeding efficiency concrete operations are as follows:
1, prepare clean sand filter seawater in advance, and be made normal temperature seawater (28 DEG C or so), medium temperature (transition) seawater (24 DEG C),
Low temperature seawater (20 DEG C) each 30L is spare.
2, sperm is collected under normal temperature condition.It distributes all Che oysters hastened parturition to ID number, they is placed in room temperature sperm respectively
Bucket (carry out semen collection) is discharged, after serotonin injection, starts within 5 minutes spermiation occur, when spermiation is about 2-3
After minute, removes Che oyster and 500 mesh sieve tulles of the sperm are filtered into tissue block and other sundries, filtrate is sperm, label
Sperm number " ♂+ID ".Che oyster is placed in intermediate bucket (ID is corresponding) 1 minute for filling medium temperature seawater simultaneously, then takes out and is set
Discharge bucket in the ovum for filling low temperature seawater (ID number is corresponding).
3, recovery of ova under cryogenic conditions.Che oyster is allowed to continue discharge sexual gland in the ovum discharge bucket for filling low temperature seawater mixed
It closes product (ovum) 15-20 minutes, after discharge is basic, obtains ovum liquid, the taking-up of Che oyster is cleaned repeatedly with medium temperature seawater, so
It is placed on renewal cultivation pond.Ovum liquid is filtered with 300 mesh sieve tulles simultaneously, removes the sperm to lose vigor, ovum is located at bolting silk net
On, ovum is placed in preprepared normal temperature seawater by recovery of ova, record corresponding giant clam ID number, ovum be identified as " ♀+
ID”。
4, the room temperature of ovum restores.The ovum being collected into is respectively placed in the bucket for filling room temperature filtering sea according to label
It is interior, restore 5 minutes in case fertilization, ovum are identified as " ♀+ID "；
5, microexamination essence oogamete quality abandons gamete inferior, accordingly as needed by the different sperm in source and ovum
Artificial insemination processing is carried out at normal temperature.
By fully controllable mode in the present embodiment, heterologous insemination obtains Che oyster fertilization rate reached to 90%, hatching rate
85% or more ideal expected results.
Claims (3)
1. a kind of effectively avoid the autogamous physical method of giant clam shellfish, which is characterized in that all giant clam distribution ID numbers hastened parturition,
They are placed in respectively in the spermiation bucket for filling normal temperature seawater, after spermiation 2-3 minutes, remove giant clam and with 500 mesh
Bolting silk net filtration tissue block, filtrate are sperm, are marked sperm number " ♂+ID number "；The giant clam shellfish of removal is placed in simultaneously in filling
The intermediate bucket of warm seawater 1 minute then takes out and is placed in the ovum for filling low temperature seawater discharge bucket, allows Che in ovum discharge bucket
Giant clam continues discharge ovum 15-20 minutes, and after discharge, giant clam is taken out, and obtains ovum liquid, while being filtered with 300 mesh sieve tulles
Ovum liquid, ovum are located in bolting silk net, recovery of ova, record ovum marked as " ♀+ID number ", by the ovum being collected into according to label
It is respectively placed in the bucket for filling normal temperature seawater, restores in case fertilization；Gamete inferior is abandoned after checking smart oogamete quality, accordingly will
The different sperm in source and ovum carry out artificial insemination processing as needed.
2. according to claim 1 effectively avoid the autogamous physical method of giant clam shellfish, which is characterized in that the sea
Water is all sand filtration seawater, and described take out giant clam is to clean giant clam taking-up repeatedly with medium temperature seawater, is subsequently placed in recovery training
Support pond.
3. according to claim 1 effectively avoid the autogamous physical method of giant clam shellfish, which is characterized in that described is normal
The seawater that warm seawater is 28 DEG C, the seawater that the medium temperature seawater is 24 DEG C, the seawater that the low temperature seawater is 20 DEG C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610454759.6A CN106035166B (en) | 2016-06-20 | 2016-06-20 | It is a kind of effectively to avoid the autogamous physical method of giant clam shellfish |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610454759.6A CN106035166B (en) | 2016-06-20 | 2016-06-20 | It is a kind of effectively to avoid the autogamous physical method of giant clam shellfish |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106035166A CN106035166A (en) | 2016-10-26 |
| CN106035166B true CN106035166B (en) | 2019-03-08 |
Family
ID=57167810
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610454759.6A Active CN106035166B (en) | 2016-06-20 | 2016-06-20 | It is a kind of effectively to avoid the autogamous physical method of giant clam shellfish |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106035166B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110637763B (en) * | 2019-09-26 | 2021-05-04 | 中国科学院南海海洋研究所 | Preparation method of tridacna shell morphology and mantle color character interchange strain |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101785437B (en) * | 2009-11-24 | 2011-12-07 | 广东海洋大学 | Chlamys nobilis offspring seed cultivation method |
| CN102077800A (en) * | 2010-12-20 | 2011-06-01 | 山东海益宝水产股份有限公司 | Method for breeding oyster |
| CN103229739B (en) * | 2013-05-17 | 2014-06-11 | 琼海时达渔业有限公司 | Artificial induced spawning method of tridacna shell |
| CN103238542B (en) * | 2013-05-17 | 2014-09-17 | 琼海时达渔业有限公司 | Bottom sowing culture method of giant clams |
| CN103828746B (en) * | 2014-03-04 | 2016-01-20 | 大连海洋大学 | A kind of mating system of Ruditapes philippinarum |
| CN104823889B (en) * | 2015-05-20 | 2017-08-25 | 莱州市曙光渔业有限公司 | A kind of breeding method of scallop hybrid breeding |
| CN104885978B (en) * | 2015-06-02 | 2017-04-19 | 大连海洋大学 | Method for breeding by aid of one-year-old patinopecten yesoensis |
| CN105075938A (en) * | 2015-06-30 | 2015-11-25 | 福建省水产研究所 | Large-scale cultivation method for Tapes dorsatus spats |
-
2016
- 2016-06-20 CN CN201610454759.6A patent/CN106035166B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN106035166A (en) | 2016-10-26 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Mao et al. | Bivalve production in China | |
| Pavlov et al. | Reproductive dynamics | |
| CN1957686B (en) | Method of breeding pearls by using 6-dimethylin purine to induce triploid jibheader clams | |
| Shan et al. | Novel means for variety breeding and sporeling production in the brown seaweed U ndaria pinnatifida (P haeophyceae): Crossing female gametophytes from parthenosporophytes with male gametophyte clones | |
| CN105145419A (en) | Breeding method for obtaining hybrid advantages of hippocampus kelloggi | |
| CN101530064A (en) | Urechis unicinctus breeding and cultivating method | |
| CN103918595A (en) | Silver carp breeding method | |
| CN103766253A (en) | Method for breeding small-sized shrimps | |
| CN106035166B (en) | It is a kind of effectively to avoid the autogamous physical method of giant clam shellfish | |
| JP2011244795A (en) | Method for producing on land of sand-submerged bivalves, such as littleneck clam | |
| CN101627733A (en) | Method capable of promoting stripped shellfish eggs to be mature in vitro | |
| Chávez‐Villalba et al. | Worldwide overview of reproduction, juvenile collection, spat production and cultivation of pen shells | |
| CN104322413B (en) | A kind of Gymnocypris prezewalskii fry high-density breeding method | |
| CN107265653B (en) | Method for promoting growth of submerged plants by using fish and benthonic animals in synergy mode | |
| CN102388825A (en) | Method for cultivating juvenile rapana venosa in artificial breeding | |
| Dao et al. | Scallop sea bed culture in Europe | |
| CN104206334A (en) | Culture method of Mexico and Korea hybrid scallops | |
| CN105941233B (en) | One kind effectively avoiding the autogamous chemical method of giant clam shellfish | |
| Strand et al. | Perspectives on bivalves providing regulating services in integrated multi-trophic aquaculture | |
| Moss et al. | Disease prevention strategies for penaeid shrimp culture | |
| CN102986571A (en) | Inducing method for gynogenetic diploid of Nibea albiflora | |
| CN102440207A (en) | Establishing method of Cnobilis inbred line | |
| CN112205330A (en) | Large-scale artificial breeding method of Liriosa pigeonia | |
| CN105557589A (en) | Low-salt breeding technical method for epinephelus akaara fries | |
| Fetherman et al. | Optimizing triploid walleye production in Colorado |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |