CN107087560A - Penaeus Vannmei and the foundation and its application of golden pomfret ecological polyculturing pattern - Google Patents

Abstract

The invention discloses a kind of Penaeus Vannmei and golden pomfret ecological polyculturing pattern, it is characterised in that：With the culture pond of 1.8~2.0m water can be injected, the culture pond area is more than 3 mu, and the culture pond has independent culvert system, and thorough draining is carried out to the culture pond before cultivation, fish for circular groove sludge, pond bottom beach face fully turns over exposure, and golden pomfret puts the previous moon water inlet of seedling, is uniformly splashed pond bottom with bleaching powder slurrying, put before seedling 15 days, ramulus mori is laid at pond bottom, the ramulus mori is immersed in pond bottom, ecological polyculturing pattern and has raised together with Penaeus Vannmei and golden pomfret.Present invention improves Penaeus Vannmei and the water ecological environment of golden pomfret ecological polyculturing pattern, so as to improve the survival rate and yield of Penaeus Vannmei and golden pomfret.

Description

Penaeus Vannmei and the foundation and its application of golden pomfret ecological polyculturing pattern

Technical field

The present invention relates to the foundation and its application of a kind of Penaeus Vannmei and golden pomfret ecological polyculturing pattern.

Background technology

Mixed breed is studied and utilized as current aquaculture before the particularly important form in field, hundreds of or even more than one thousand years Various forms of Fish-shrimp mix-cultures have been carried out in cultivating pool system including multiple Asian countries including China.But it is straight Raising together with for prawn and mullet is successfully made to the Ganyu of Jiangsu in 1979, mixed breed just begins to take shape in China, and gradually sends out Exhibition is grown and is further improved.

Mixed breed be according to the circulation of niche complementarity, inter-species symbiosis and material and the ecological principle such as energy flow, By various aquaculture organism of mutual reciprocity and mutual benefit according to certain quantity, density, specification and the reasonably combined cultivation of proportionate relationship same Production form in system.Common aquaculture model of raising together with typically has fishes and shrimps, ichthyophagy crab, fish shellfish, fish algae and fish shrimp crab shellfish algae 5 Plant Polyculture.Various natural baits and man-made feeds in system can be made full use of by raising together with, and that improves matter and energy utilizes effect Rate；Meanwhile, it is biological between make system that there is stronger self-control and resist outside by the mechanism such as mutually restricting, converting and feed back Come the ability disturbed, the problems such as traditional aquaculture model wasting of resources, environmental pollution and ecosystem fragility are successfully overcome.

Egg-shaped pompano delicious meat, feeding habits are simple, growth is quick, premunition is strong, and general cultivation can reach for 4-5 months City's specification.China Taiwan in 1989 carries out artificial breeding and successful, subsequent Fujian, Guangdong, Hainan to egg-shaped pompano seed Etc. ground start cultivation, and scale gradually increases, and has a variety of breeding way such as pond, buoyant raft and net cage.Match somebody with somebody because it can largely be used Feed is closed, remarkable in economical benefits is well received, be breeding prospect preferably one of excellent rare fingerling.In the height by the several years After speed development, unreasonable due to being laid out, cultivation density is too high, has had resulted in the deterioration of cultivation water environment and ecological environment, has led Cause egg-shaped pompano disease increasingly serious, product quality declines, culture benefit go fromes bad to worse.Domestic and foreign scholars are carried out to egg-shaped pompano Substantial amounts of research, such as poplar fire is contained in the egg-shaped pompano of research and probe in 2006 and propagates such as temperature, salinity and dissolved oxygen artificially Optimal growth conditions.Biology and cultural technique also to egg-shaped pompano be described in detail and study (Liu Chubin, 2009).Xia Liqun etc. has also carried out detailed overview etc. in 2012 years to egg-shaped pompano Major Diseases and its progress.But it is big The introduction focused mostly in terms of breed, disease pathology, Physiology and biochemistry, molecular labeling, rarely found egg-shaped pompano mixing is supported The research report for grow effect on environment, particularly cultivating system nitrogen phosphorus revenue and expenditure, converting and utilizing.

Aquaculture be in the cultivating system of manual intervention, by cultivation object carry out bait throwing in, fertilising or merely with Natural bait etc., reaches the purpose for improving yield, obtaining a large amount of aquatic products.Every kind of cultivation object generally adapts to certain environment bar Part, such as environmental condition exceed its accommodation, and cultivation object is just difficult to grow, breeds even dead.The extensive cultivation of tradition is usual With higher ecological benefits, high density culture then has higher economic benefit, and mixed breed can then take into account economical and raw very well State benefit.But also to there is variety matching, stocking size time and aquaculture model portability etc. in the latter many in production practices Problem.Therefore, when progress aquaculture production is put into practice, the Ecology for wouling have to further investigation cultivation object is special Levy, and then grasp and utilize its rule of life and the relation with surrounding environment, for the sustainable aquaculture clothes of development efficient health Business.

Golden pomfret, i.e. egg-shaped pompano (Trachinotus ovatus) are under the jurisdiction of Osteichthyes (Osteichthyes), perch Shape mesh (Perciformes), Anabantoidei (Percoidei), Scad sections (Carangidae) silvery pomfret Scad subfamilies (Trachinotinae) silvery pomfret Scad belongs to (Trachinotus), is commonly called as golden silvery pomfret, ovum silvery pomfret, yellow cured silvery pomfret etc., is distributed widely in Southeast Asia tropical and subtropical zone marine site, America Tropical temperate zone Atlantic Ocean seashore and coastal area of china.Feeding habits are simple, growth is quick, premunition is strong, in general cultivation 4-5 months City.Area is supported based on Hainan, Guangdong and Fujian etc., there are a variety of breeding way such as pond, buoyant raft and net cage, largely can be raised using cooperation Material, remarkable in economical benefits is well received, is breeding prospect preferably one of excellent rare fingerling.Research shows that egg-shaped pompano is Wide salt water warm straddling fish stocks at the middle and upper levels, its adapt to more wide in range alkalescence (pH7.6-9.6) water environment, salinity (3 ‰- 33 ‰) and temperature (16-36 DEG C, most it is suitable be 22-28 DEG C).But grown in high salinity seawater it is poor, salinity be less than 20 ‰ when give birth to It is long rapid, it is slow-growing during more than 20 ‰.Its low temperature tolerance ability is also poor, and minimum critical-temperature is only 14 DEG C, surpasses two days later Death just occurs in 14 DEG C of temperature below；When water temperature drop is to less than 16 DEG C, just enter people's Wintering Period (generally late December extremely Early Febuary in next year) and stop ingesting；16-18 DEG C is then ingested on a small quantity；Only just ingested in more than 22 DEG C of water temperature strong.Also because This its can in water ceaselessly movable oxygen consumption, cause its minimum critical DO also higher (about 2.5mg/L).Egg-shaped pompano is omnivorous Partially carnivorous, various based on copepod larva of the larva and juvenile just hatched under nature swim with benthon as food；It is young Take food water flea, Polychaeta and amphipoda etc. more juvenile fish, the fish blended, shrimp can be then fed when growing to 2cm rotten；When young adult fish Phase, in addition to mollusk and the fish shrimp crab young etc. for food, it can also feed appropriate special feed；The throat plate of adult fish is quite sent out Reach, the biologies such as clam, spiral shell can be taken food.And propagate egg-shaped pompano artificially and generally ingested on daytime, it is heavy, digestion is fast, it is fierce to rob food, but Without mutually killing and devouring phenomenon, often in the morning or dusk feeds expanded buoyancy marine fish feed.

Penaeus Vannmei (Penaeus vannamei) is under the jurisdiction of Arthropoda (Arthropoda), Crustachia (Crusracea), Decapoda (Decapoda), swimming suborder (Narantia), Penaeidae (Penaeidae), Penaeus (Penaeus), white shrimp subgenus (Litopenaeus), also known as white limb shrimp, white shrimp and Penaeus vannamei.In originating in, South America Pacific warm water waters, NATURAL DISTRIBUTION is littoral with Ecuador in south of Peru to Mexico Sang Nuola regions following the line of the sea Concentrate the most.Rapid, adaptable and resistance to high-density breeding is grown, general 90d can be formed, it is right with Penaeus monodon, China Shrimp is listed as the big excellent shrimp species of current world's cultured output highest three.Like shale bottom under natural conditions to dwell, pH value requires to maintain 8.0 or so；DO is in more than 5mg/L, minimum 1.2mg/L；It can survive in 15-40 DEG C of water environment, 20-30 DEG C is suitable raw Long temperature；Usual salinity requirements can be raised in 2 ‰ fresh water after 10-34 ‰, but domestication.Penaeus Vannmei is omnivorous inclined animal Feeding habits, the young is using the planktons such as diatom, fairy shrimp and wheel animalcule and zoobenthos as food；Into shrimp then with mollusk, algae Insect and microcrustacean etc. are food.It is relatively low to the nutritional need of feed under Artificial feeding conditions, but will to feed curing degree Ask higher, if in bait the ratio of protein component account for more than 20% prawn can normal growth and breeding.Meanwhile, it may be used also Fully decompose and utilize the organic debrises such as residual bait, excrement and corpse in cultivating system.

Fishery technical centre for spreading Pan Qing grades clearly in Zhejiang Province Sanmen County establish Penaeus Vannmei in Sanmen County and mixed with golden pomfret The pattern of supporting, the characteristic of its ingested using golden pomfret algae and zooplankter in water, is played improvement water quality and reduces pathogen and propagated Effect, to reduce cultivation Penaeus Vannmei disease generation, reach efficient ecological cultivation purpose.The present inventor passes through south The exploration of penaeus vannamei and golden pomfret Polyculture, obtains the firsthand information that Penaeus Vannmei is raised together with golden pomfret, to adjustment Zhejiang Province's aquaculture industrial structure, promotion aquaculture diversification and sustainable development are significant.

The content of the invention

The present invention is on the basis of the pattern that Zhejiang Province Sanmen County Fishery technical centre for spreading team is set up, to be applied To Shaoxing, and based on market need, set up in Penaeus Vannmei and golden pomfret ecological polyculturing pattern, practical application encounter it is various Problem, especially for the requirements at the higher level of golden pomfret and Penaeus Vannmei to oxygen in water are met, the present invention enters to the pattern Improvement is gone.

The improved method is that ramulus mori is placed at pond bottom, and ramulus mori is rich in a variety of nutrients such as pectin, cellulose, crude protein Matter, its contained element is as shown in table 1.According to surveying and determination, ramulus mori bast contains crude protein 5.44%, cellulose 51.88%, lignin 18.18%, hemicellulose 23.02%, ash content 1.57%.Xylem contains lignin 19.11%, pentosan 21.76%, hemicellulose Element 78.83%, ash content 2.32%.Chemical composition species is more contained by ramulus mori, mainly there is flavone compound, alkaloid, polysaccharide With Coumarins compound.Also containing amino acid, organic acid, volatile oil and multivitamin etc.；In addition, some are rubbed matter, dissociated Sucrose, glucose, xylose, maltose, stachyose, fructose, raffinose, arabinose, long shot acid and gland drift cry of certain animals exist in In ramulus mori.

The element composition of the ramulus mori of table 1

Composition	N	C	H	O	Ash content
						Content (%)	0.4	44.25	5.56	49.41	0.38

The present invention operatively improves oxygen in water (DO), improves South America white by placing ramulus mori in fishpond Prawn and the water ecological environment of golden pomfret ecological polyculturing pattern, so as to improve survival rate and the production of Penaeus Vannmei and golden pomfret Amount, creates more preferable economic benefit.

Therefore, the present invention is provided：

(1) a kind of Penaeus Vannmeis of and golden pomfret ecological polyculturing pattern, it is characterised in that：

With that can inject the culture pond of 1.8~2.0m water, the culture pond area is more than 3 mu, the culture pond tool There is independent culvert system,

Thorough draining is carried out to the culture pond before cultivation, circular groove sludge is fished for, pond bottom beach face fully turns over exposure, gold Butterfish puts the water inlet of the seedling previous moon, is uniformly splashed pond bottom with bleaching powder slurrying,

Put before seedling 15 days, ramulus mori laid at pond bottom, the ramulus mori is immersed in pond bottom,

Penaeus Vannmei and golden pomfret are raised together with the ecological polyculturing pattern.

(2) ecological polyculturing patterns of the as described in (1), contains

500 tails of golden pomfret/mu~1500 tails/mu；1,0000 tails of Penaeus Vannmei/mu~2,0000 tails/mu.

(3) ecological polyculturing patterns of the as described in (1), wherein,

The ramulus mori is bundled into a bundle per 10kg, often ties and is fixed on pond bottom by weight.

(4) ecological polyculturing patterns of the as described in (3), wherein,

The usage amount of the ramulus mori is more than 10 bundles/mu.

Brief description of the drawings

Fig. 1 ecological polyculturing pattern diagrams of the embodiment of the present invention.

The test result of dissolved oxygen concentration in Fig. 2 A groups of embodiment of the present invention pond (D groups are control).

The test result of dissolved oxygen concentration in Fig. 3 B groups of embodiment of the present invention pond (D groups are control).

The test result of dissolved oxygen concentration in Fig. 4 C groups of embodiment of the present invention pond (D groups are control).

Embodiment

First, materials and methods

1. experimental design

The fish pond of the present invention in real time for Shaoxing Ming Cheng agricultural science and technologys development corporation, Ltd., 1,100 mu of pond culture area, Single 10 mu of pond, wherein 200 mu of steel structure greenhouse, 100 mu of high standard pond has put into 2,0,000,000 yuan of construction fund, base so far Ground is Zhejiang Province fishery leading industry demonstration area.Test tank size is 10 mu, and water salinity is 15.Select 10 mouthfuls of ponds totally 100 Mu is used to test, wherein 500 tails of golden pomfret/mu, and 20,000/mu Penaeus Vannmei (A groups) are raised together with 3 mouthfuls of pools；Raise together with gold in 3 mouthfuls of pools 1,000 tails of butterfish/mu and 1.5 ten thousand/mu Penaeus Vannmei (B groups)；Raise together with 1500 tails of golden pomfret/mu and 10,000/mu South America in 3 mouthfuls of pools White shrimp (C groups)；1 mouthful of pool monoculture Penaeus Vannmei is the control pool (D groups).Specific experiment group is as shown in table 2.

Table 2 each embodiment puts situation and oxygenation measure in a suitable place to breed

2. test method

(1) sterilization is leveled in pond：The general dry method that carried out with 150,000 grams/acre of quick lime is cleaned up the pond sterilization.If disappearing with water Poison, with 1.0~2.0 kgs/m³Water body quick lime or the sterilization of the bleaching powder of 50~70 grams m 3, thoroughly kill harmful organisms, cause a disease Intermediate host that is biological and carrying pathogen.

(2) Penaeus Vannmei seed is put in a suitable place to breed：Penaeus Vannmei seed puts that seedling separation kind mark is thick and two stages of seedling separation in a suitable place to breed, shrimp Seedling is first put into cement pit and temporarily supports mark slightly, 400 meters of every mouthful of cement pit², breeding density is every mouthful of tail of pond 2,000,000.By 20~30 days Temporarily to support mark thick, shrimp seedling specification carries out seedling separation when reaching 3 centimetres of body length, into the high-elevation breeding pond stage, wherein annual spring the One batch is 180,000~200,000 tails/mu with the 3rd batch of shrimp seedling feed coefficient breeding density of winter, and summer, second batch of shrimp seedling breeding density was 130000~150,000 tails/mu.Water temperature is 20 DEG C or so when putting in a suitable place to breed.In 15 days of the temporary nutrient after seedling of shrimp seedling, one was checked every 2~3 days The cultivation density of secondary shrimp seedling, it is found that when shrimp seedling breeding density is diluter, ascertain the reason and supplement shrimp seedling in time.

(3) fry being put in a suitable place to breed and temporarily supports：Golden pomfret seedling is purchased from Shaoxing Zhong Tian aquatic products technical research institute, for the Chinese platform of the reason Gulf is introduced, 1.2~1.3 centimetres of fry body length during introduction.After fry is first temporarily supported 1~2 week after introducing, fish body is used when being put into shrimp ponds Disinfecting solution of potassium permanganate, to reduce pathogen cross transmission.Because of more than 16 DEG C of fry temperature of cultivation, according to the water temperature in Zhejiang Province Situation, is raised together with being late May since second batch of cultivation of Penaeus Vannmei.Golden pomfret is surveyed during Penaeus Vannmei harvesting Production, while golden pomfret is temporarily supported, treats to raise together with next batch of Penaeus Vannmei, and blending supports 2 batches to mid-November.

(4) feed and its feed：Penaeus Vannmei selects high-quality mixed bait, according to weather, survival rate, residual bait amount, strong Health situation, aquatic environment, shell situation, medicining condition and biological feed amount determine daily ration, feeding quantity.The bait throwing in of timing daily 4 times, when Between be respectively 6:00、11:00、16:30、21:30.The golden pomfret raised together with does not feed feed individually, Penaeus Vannmei of mainly ingesting Remaining feed, excrement and algae.

(5) regulating and controlling water quality：Major measure includes refreshing the water periodically, intake will be by strict sterilization processing, mechanical enhancement, regular Splash quick lime etc., by targetedly carrying out regulating and controlling water quality, water transparency is maintained at 20~30 centimetres, ammoniacal nitrogen contains Measure below 0.5 mg/litre, and keep stablizing relatively for water quality physical and chemical index and biomass.

(6) daily management：It must adhere to patrolling pond, should not be less than 4 times daily, and carry out water quality, bait consumption, shrimpfish shape Condition, pond bottom color, record situations such as fish does harm in pond, discovery situation is taken emergency measures in time to be dealt carefully with.

(7) disease control：, although the incidence of disease is relatively low, also there is the generation of fish and shrimp diseases in the pond of Fish-shrimp mix-culture.Cultivated Cheng Zhong, mainly passes through the generation of the pre- disease preventions of measure such as pond desilting sterilization, seed Quarantine disinfection, regular water disinfection.To hair The pond of sick evil, forbids using medicines such as organic phosphates, desinsection chrysanthemum esters when treating disease.

(8) fish for：Penaeus Vannmei is fished for lobster basket, shrimp drains water body fishing again after the completion of fishing for substantially.

(9) monitoring of associated field hydration index is determined：Every 15 days or so in 9:00-11:00 tests at each respectively Pond Tou Si areas, offshore water J, 4, central area Z, JZ midpoints area, which layout, carries out the survey such as dissolved oxygen (DO), water temperature (T), pH, salinity (S) It is fixed, and breeding water body top layer, bottom water sample are gathered with hydrophore, the layout water sample of acquisition of the identical water layer difference in identical pond is filled Divide and mixed sample is obtained after being well mixed.Reference《Marine monitoring specification》With《Cultivation water environment chemical experiment》.Wherein, dissolved oxygen (DO) using portable dissolved oxygen instrument JENCO LD-900-X；(Shenzhen is bright using portable pH meter and thermometer for pH, temperature (T) Sincere Industrial Co., Ltd.).

Embodiment

Implemented according to above-mentioned experimental design and test method, pollution-free source in 3 kilometers of culture zone periphery, water source fills Foot, irrigation and drainage are convenient.Culture pond be in prawn culturing area in flakes, 10 mu of area/only, can water filling depth 1.8-2.0m, Chi Xingwei Rectangle, with independent culvert system, water inlet is on dyke, and discharge outlet is in the middle of the bottom of pond, and flat pond bottom is in pot Bottom shape.Oxygen increasing equipment supports shrimp aerator using Shandong East China HDSR50-350 aerators of breeding fish, and every mouthful of pool sets one, wherein increasing Oxygen machine begins to use from July, and daily noon starts shooting two hours, and each pond available machine time is consistent.

Spring carries out solarization pond, thoroughly drains Yu Shui, fishes for circular groove sludge, pond bottom beach face fully turns over exposure.Golden pomfret The water inlet of the seedling previous moon is put, is uniformly splashed pond bottom with bleaching powder 10~15kg/667 ㎡ slurrying.

Put before seedling 15 days, ramulus mori (reference examples are willow branch, abbreviation withy) is laid at pond bottom, the ramulus mori is purchased from Zhejiang Huzhou City peasant household is saved, the ramulus mori after winter felling is bundled into 10kg a bundles, and binding rope uses the not perishable nylon not readily dissolved Rope or elastic string, the ramulus mori tied randomly places pond bottom, can also be emitted on pond bottom according to certain rule, preferably according to Certain regular-scattered arrangement.The movement of ramulus mori during to prevent from pouring water, can use weight, and such as stone or iron block are fixed, this implementation The 5kg fragments of brick used in example, which link together, places pond bottom.In the present embodiment, 50 bundle 10kg mulberry is placed in 10 mu of ponds Same amount of withy, withy buying rural area from Shaoxin City are put into branch, reference examples.The ramulus mori and withy are in the winter More than 2 months season non-spraying pesticide.

Spread after above-mentioned branch, intake with the bolting silk bag filtration of 80 mesh, 100cm of intaking, the selection fine day morning applies nitrogen phosphorus fertilizer Plus bio-fertilizer, every 667 ㎡ nitrogen fertilizer amounts 2kg, phosphate fertilizer 5kg, bio-fertilizer 2kg, water is cultivated into yellow green or Sandy.

May 21 delivered Penaeus Vannmei seedling, the long 1.2cm of Penaeus Vannmei body.Delivered golden pomfret seedling, seed rule on June 3 Lattice golden pomfret 5cm/ tails or so.Started June 1 to measure water quality data, measurement in every 10 days is once.

As a result：Dissolved oxygen (DO) excursion in three groups of ponds is 4mg/L between 6.5mg/L, having substantially met golden silvery pomfret The cultivation of fish and Penaeus Vannmei needs.Shown from Fig. 1-3 result, without significant difference between each experimental group, but in group Pond bottom is equipped with the pond of the ramulus mori of the present invention, and dissolved oxygen is significantly higher than other groups, and its reason is probably the composition in ramulus mori For the influence of microorganism in water environment, help to improve the content of dissolved oxygen.Especially in the season of 7, August part high temperature the most Section, the pond of ramulus mori has been used using the method for the present invention, higher dissolved oxygen concentration has been remained in that, so as to improve golden silvery pomfret Fish and the survival rate and the speed of growth of Penaeus Vannmei.In addition, the pH value during each experiment in pond is between 8-8.3, respectively Business notable difference between group, meta-alkalescence water quality also meets golden pomfret and Penaeus Vannmei growth demand.Also all exist in terms of water temperature Within normal cultivation range of needs, its data statistics is saved herein.

Finally according to cultivation during golden pomfret dead fish mantissa, count the survival rate of golden pomfret, and finally harvest The statistical result of golden pomfret and Penaeus Vannmei is as shown in table 3.

The yield of the golden pomfret survival rate of table 3 and golden pomfret and Penaeus Vannmei

It can be outputed by table 3, the culture pond of ramulus mori shop fixtures is employed in the embodiment of the present invention, the survival rate of golden pomfret is notable Higher than other culture ponds without ramulus mori shop fixtures or be culture pond using withy shop fixtures.The cultivation of ramulus mori shop fixtures in each group The golden pomfret product on the pool and the product of Penaeus Vannmei are also significantly greater than with the other ponds of group.It has been recognised by the inventors that ramulus mori is special Composition improves the content of dissolved oxygen except that can improve water environment microorganism, so as to improve golden pomfret and Penaeus Vannmei Yield, in addition the special composition of ramulus mori be possible to directly enhance the resistance against diseases of golden pomfret and Nan Baimei prawns in itself.Although Have been reported that and be incorporated into after crushing ramulus mori or mulberry leaf etc. among the feed of aquaculture, the active ingredient in mulberry tree is utilized with it The resistance against diseases of fishes and shrimps is improved, but directly ramulus mori is taped against into culture pond bottom is the trial first of the present inventor, and achieve meaning Effect unexpectedly.Ramulus mori is directly taped against culture pond bottom, it is not only simple efficient, also save production cost.Directly as fish During shrimp feed additive, have fishes and shrimps and produce the sorrow of rejection, but be taped against culture pond bottom, the active ingredient of ramulus mori is helped indirectly The yield for improving cultivation is helped, is that raiser increases income and brings significant effect.

Claims (4)

1. a kind of Penaeus Vannmei and golden pomfret ecological polyculturing pattern, it is characterised in that：

With the culture pond of 1.8~2.0m water can be injected, the culture pond area more than 3 mu,

The culture pond has independent culvert system,

Thorough draining is carried out to the culture pond before cultivation, circular groove sludge is fished for, pond bottom beach face fully turns over exposure,

Golden pomfret puts the water inlet of the seedling previous moon, is uniformly splashed pond bottom with bleaching powder slurrying,

Put before seedling 15 days, ramulus mori laid at pond bottom, the ramulus mori is immersed in pond bottom,

Penaeus Vannmei and golden pomfret are raised together with the ecological polyculturing pattern.

2. ecological polyculturing pattern as claimed in claim 1, contains

500 tails of golden pomfret/mu~1500 tails/mu；1,0000 tails of Penaeus Vannmei/mu~2,0000 tails/mu.

3. ecological polyculturing pattern as claimed in claim 1, wherein,

The ramulus mori is bundled into a bundle per 10kg, often ties and is fixed on pond bottom by weight.

4. ecological polyculturing pattern as claimed in claim 3, wherein,

The usage amount of the ramulus mori is more than 10 bundles/mu.