CN103004701B - Indoor raising method for perinereis aibuhitensis by circulating water - Google Patents
Indoor raising method for perinereis aibuhitensis by circulating water Download PDFInfo
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- CN103004701B CN103004701B CN201210577849.6A CN201210577849A CN103004701B CN 103004701 B CN103004701 B CN 103004701B CN 201210577849 A CN201210577849 A CN 201210577849A CN 103004701 B CN103004701 B CN 103004701B
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Abstract
The invention relates to an indoor raising method for perinereis aibuhitensis by circulating water. The method comprises the following steps: 1) preparing for raising; 2) circulating raising water and supplying oxygen; 2) stocking; 3) feeding; 4) controlling a raising environment; and 5) timely sorting out the ill, disabled and weak perinereis aibuhitensis during the raising process. The indoor raising method for the perinereis aibuhitensis by circulating water provided by the invention is formed by improving through adopting a circulating water raising mode on the basis of the perinereis aibuhitensis seawater raising. Under the precondition of ensuring the vigor of the perinereis aibuhitensis, the survival time of the perinereis aibuhitensis is prolonged, and the raising density is increased.
Description
Technical field
The present invention relates to a kind of method of storing up of perinereis aibihitensis Grube, particularly, at the indoor technology of the storing up cultivation perinereis aibihitensis Grube that utilizes cycle water, belong to mariculture technical field.
Background technology
Perinereis aibihitensis Grube (
perinereis aibuhitensis) belong to Annelida, polychaeta, Nereidae, happiness is lived in caves in calm, nutritious silt particle beach, intertidal zone, is the sociales of silt beach, China intertidal zone biology, is also the main composition biology of CHINESE OFFSHORE bottom community, has the important ecological value.
Clam worm is the high-quality bait of fish, shrimp, crab etc., and nutritive value is abundant, is called as " omnipotent bait ", has high economic worth.The C.I.F. of Japanese market is per ton up to tens thousand of dollars at present, and very worthy equally in market, West Europe, supply falls short of demand.
Perinereis aibihitensis Grube has the features such as individuality is large, protein content high (70%-80%), is the important kind that can develop in Nereidae animal, has wide development prospect.In recent years, domestic existing many enterprises and institute have carried out the cultural technique research of perinereis aibihitensis Grube in succession.It is pool cultivated that main breeding way has mudflat aquaculture, the cultivation of batch production cement pit and shrimp at present.These three kinds of modes all survive in clam worm in " mud bank " environment.In the time that clam worm reaches commercial specification, (approximately 200/kg), need excavate out from " mud bank ".The clam worm that lives is transported to dealer place, often need in briny environment, store up it, to extend the clam worm time-to-live.The technology of storing up using at present only can make clam worm survival about 7 days, and a large amount of clam worms are because of the change death of environmental condition.This has just limited the sales cycle of clam worm alive, increases the business risk of Liao Huo clam worm distributors.Utilize the current technology of storing up, because the clam worm time-to-live is short, the correlative study having limited taking perinereis aibihitensis Grube as experiment sample is carried out simultaneously.
Summary of the invention
For the defect existing in prior art, the object of the present invention is to provide one efficiently, indoor circulating water is stored up perinereis aibihitensis Grube technology easily.
For reaching above object, the technical scheme that the present invention takes is:
A kind of perinereis aibihitensis Grube indoor circulating water is stored up method, adopts following steps:
1) store up preparation
Storing up in case, what to inject salinity be 26 stores up water, height of water level 40cm;
2) water, oxygen supply are stored up in circulation
Store up water with water circulating pump circulation, controlled circulation water volume flow rate is about 0.005m
3/ h, rate of water exchange per hour is about 25%; Use aeration pump, maintain and store up in water body more than dissolved oxygen content is always 10mg/L;
2) put in a suitable place to breed
The density of storing up according to 100/L puts perinereis aibihitensis Grube storing up storing up in water in case in a suitable place to breed;
3) bait throwing in
Throw in the Powdered shrimp pellet filtering through 60 order mesh screens, protein content 30%～35%, the frequency of throwing something and feeding is to throw something and feed once for every two days, single feeding volume is 4g/100 bar;
4) culture environment control
It is 15 ± 1 DEG C that temperature is stored up in control; Illumination is about 1000 μ molm
-2s
-1, light dark period is than being 12:12;
5) store up sick, residual, the weak perinereis aibihitensis Grube of timely cleaning in process.
On the basis of such scheme, described in to store up case be vinyon material.
On the basis of such scheme, described in store up case and soak 24h with 1% watery hydrochloric acid before use, use afterwards the K of 20mg/L
2mnO
4sterilization, finally cleans with clear water.
On the basis of such scheme, described in to store up water be that the allotment of nature seawater and running water forms.
On the basis of such scheme, described nature seawater after 7 days quiescent settings, then with aperture be 45 μ m fibrous filter membrane filter, 121 DEG C afterwards, 20min sterilization treatment.
On the basis of such scheme, described running water is placed on the position of well-ventilated, illumination abundance, does not stop aeration through 7 days.
On the basis of such scheme, described in store up after water prepares add BZT(ammonium nitrate), making its concentration is 0.3PPM.
Illustrate: BZT is conventional probiotics in the production of U.S. BIO-FormL.L.C. (Bi Wofeng biotech firm) company, mariculture, and it is proportionally configured and form by microbial composite bacteria+biology enzyme, has the effect at the clear end of water purification.BZT(ammonium nitrate) be the one in many BZT products, nitrite and the ammonia nitrogen in degrading cultivation water rapidly, effectively suppresses the breeding growth of noxious bacteria.
A kind of perinereis aibihitensis Grube indoor circulating water method of storing up of the present invention is to store up as basis with perinereis aibihitensis Grube seawater, through improveing and form by the cycle water mode of storing up.Improved and stored up density, it is normal that vigor keeps, and effectively extends the time of perinereis aibihitensis Grube at indoor culture, improved and supported temporarily density.
Embodiment
Method in following embodiment, if no special instructions, is conventional method.
Percentage composition in following embodiment, if no special instructions, is quality percentage composition.
Embodiment 1
A kind of perinereis aibihitensis Grube indoor circulating water is stored up method, adopts following steps:
1) store up preparation
Storing up in case, what to inject salinity be 26 stores up water, height of water level 40cm;
2) water, oxygen supply are stored up in circulation
Store up water with water circulating pump circulation, controlled circulation water volume flow rate is about 0.005m
3/ h, rate of water exchange per hour is about 25%; Use aeration pump, maintain and store up in water body more than dissolved oxygen content is always 10mg/L;
2) put in a suitable place to breed
The density of storing up according to 100/L puts perinereis aibihitensis Grube storing up storing up in water in case in a suitable place to breed;
3) bait throwing in
Throw in the Powdered shrimp pellet filtering through 60 order mesh screens, protein content 30%～35%, the frequency of throwing something and feeding is to throw something and feed once for every two days, single feeding volume is 4g/100 bar;
4) culture environment control
It is 15 ± 1 DEG C that temperature is stored up in control; Illumination is about 1000 μ molm
-2s
-1, light dark period is than being 12:12;
5) store up sick, residual, the weak perinereis aibihitensis Grube of timely cleaning in process.
Preferably, described in, storing up case is vinyon material.
Preferably, described in store up case and soak 24h with 1% watery hydrochloric acid before use, use afterwards the K of 20mg/L
2mnO
4sterilization, finally cleans with clear water.
Preferably, described in, storing up water is that nature seawater and running water allotment form.
Preferably, described nature seawater after 7 days quiescent settings, then with aperture be 45 μ m fibrous filter membrane filter, 121 DEG C afterwards, 20min sterilization treatment.
Preferably, described running water is placed on the position of well-ventilated, illumination abundance, does not stop aeration through 7 days.
Preferably, described in store up after water prepares add BZT(ammonium nitrate), making its concentration is 0.3PPM.
Embodiment 2
According to the method in embodiment 1, selecting 500 bodies long is 15-25cm, body colour gloss, and the normal healthy perinereis aibihitensis Grube of vigor, is divided into 5 groups, 100 every group, stores up 30 days.Support temporarily result as follows:
Table 1 perinereis aibihitensis Grube indoor circulating water is stored up 30 days results
| ? | Dead (bar) | Survival rate % |
| First group | 12 | 88% |
| Second group | 14 | 86% |
| The 3rd group | 15 | 85% |
| The 4th group | 18 | 82% |
| The 5th group | 12 | 88% |
Find through storing up after 30 days, the perinereis aibihitensis Grube survival rate of storing up by the method reaches more than 80%.
Claims (1)
1. perinereis aibihitensis Grube indoor circulating water is stored up a method, it is characterized in that adopting following steps:
1) store up preparation
Storing up in case, what to inject salinity be 26 stores up water, height of water level 40cm;
2) water, oxygen supply are stored up in circulation
Store up water with water circulating pump circulation, controlled circulation water volume flow rate is 0.005m
3/ h, rate of water exchange per hour is 25%; Use aeration pump, maintain and store up in water body more than dissolved oxygen content is always 10mg/L;
3) put in a suitable place to breed
The density of storing up according to 100/L puts perinereis aibihitensis Grube storing up storing up in water in case in a suitable place to breed;
4) bait throwing in
Throw in the Powdered shrimp pellet filtering through 60 order mesh screens, protein content 30%～35%, the frequency of throwing something and feeding is to throw something and feed once for every two days, single feeding volume is 4g/100 bar;
5) culture environment control
It is 15 ± 1 DEG C that temperature is stored up in control; Illumination is 1000 μ molm
-2s
-1, light dark period is than being 12:12;
6) store up sick, residual, the weak perinereis aibihitensis Grube of timely cleaning in process;
The described case of storing up is vinyon material;
The described case of storing up soaks 24h with 1% watery hydrochloric acid before use, with the K2MnO4 sterilization of 20mg/L, finally cleans with clear water afterwards;
The described water of storing up is that nature seawater and running water allotment form;
Described nature seawater after 7 days quiescent settings, then with aperture be 45 μ m fibrous filter membrane filter, 121 DEG C afterwards, 20min sterilization treatment;
Described running water is placed on the position of well-ventilated, illumination abundance, does not stop aeration through 7 days;
Described storing up after water prepares adds BZT nitre ammonia, and making its concentration is 0.3PPM.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210577849.6A CN103004701B (en) | 2012-12-27 | 2012-12-27 | Indoor raising method for perinereis aibuhitensis by circulating water |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201210577849.6A CN103004701B (en) | 2012-12-27 | 2012-12-27 | Indoor raising method for perinereis aibuhitensis by circulating water |
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| CN103004701A CN103004701A (en) | 2013-04-03 |
| CN103004701B true CN103004701B (en) | 2014-07-30 |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103283689B (en) * | 2013-05-23 | 2014-11-12 | 山东省海水养殖研究所 | Propagation control method for indoor cultivation of Perinereis aibuhitensis |
| CN103734093B (en) * | 2013-12-30 | 2016-04-27 | 盐城工学院 | Perinereis aibihitensis Grube heteronereis is cultivated and synchronous collection method |
| CN104126548A (en) * | 2014-07-17 | 2014-11-05 | 山东省海洋生物研究院 | Method for distributing perinereis aibuhitensis grube seedlings on large-area soil pit |
Citations (5)
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|---|---|---|---|---|
| CN1802906A (en) * | 2005-11-25 | 2006-07-19 | 宁波大学 | Perinereis aibihitensis Grube manual seedling-raising method |
| CN1817121A (en) * | 2006-03-08 | 2006-08-16 | 何丽辉 | Clam worm culturing metod |
| CN101053315A (en) * | 2007-05-28 | 2007-10-17 | 大连水产学院 | Method for indoors artificial intensive culture of clamworm |
| CN102106326A (en) * | 2011-03-02 | 2011-06-29 | 盐城工学院 | Method for three-dimensional and artificial seedlings cultivation of perinereis aibuhitensis |
| CN102349474A (en) * | 2011-08-22 | 2012-02-15 | 大连海洋大学 | Method for artificially culturing perineries aibuhitensis in biological filter |
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2012
- 2012-12-27 CN CN201210577849.6A patent/CN103004701B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1802906A (en) * | 2005-11-25 | 2006-07-19 | 宁波大学 | Perinereis aibihitensis Grube manual seedling-raising method |
| CN1817121A (en) * | 2006-03-08 | 2006-08-16 | 何丽辉 | Clam worm culturing metod |
| CN101053315A (en) * | 2007-05-28 | 2007-10-17 | 大连水产学院 | Method for indoors artificial intensive culture of clamworm |
| CN102106326A (en) * | 2011-03-02 | 2011-06-29 | 盐城工学院 | Method for three-dimensional and artificial seedlings cultivation of perinereis aibuhitensis |
| CN102349474A (en) * | 2011-08-22 | 2012-02-15 | 大连海洋大学 | Method for artificially culturing perineries aibuhitensis in biological filter |
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| 双齿围沙蚕暂养与加工技术;朱丰锡等;《中国水产》;19970515(第5期);第35页 * |
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| 李信书等.不同水交换条件对双齿围沙蚕生长的影响.《水产养殖》.2006,第27卷(第6期),第1-4页. |
| 沙蚕闭合循环式养殖系统中细菌的数量及其代谢活性;马悦欣等;《大连水产学院学报》;20050930;第20卷(第3期);第175页第1.1节内容 * |
| 马悦欣等.沙蚕闭合循环式养殖系统中细菌的数量及其代谢活性.《大连水产学院学报》.2005,第20卷(第3期),第175页第1.1节内容. |
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Address after: 266000 Shandong city of Qingdao province Guizhou City Road No. 47 Applicant after: Shandong Marine Biology Institute Address before: 266000 Shandong city of Qingdao province Guizhou City Road No. 47 Applicant before: Shandong Province Marine Culture Institute |
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Free format text: CORRECT: APPLICANT; FROM: SHANDONG PROVINCE MARINE CULTURE INSTITUTE TO: SHANDONG MARINE BIOLOGY INSTITUTE |
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