CN103004701A - Indoor raising method for perinereis aibuhitensis by circulating water - Google Patents
Indoor raising method for perinereis aibuhitensis by circulating water Download PDFInfo
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- CN103004701A CN103004701A CN2012105778496A CN201210577849A CN103004701A CN 103004701 A CN103004701 A CN 103004701A CN 2012105778496 A CN2012105778496 A CN 2012105778496A CN 201210577849 A CN201210577849 A CN 201210577849A CN 103004701 A CN103004701 A CN 103004701A
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- water
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- aibihitensis grube
- perinereis aibihitensis
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Abstract
The invention relates to an indoor raising method for perinereis aibuhitensis by circulating water. The method comprises the following steps: 1) preparing for raising; 2) circulating raising water and supplying oxygen; 2) stocking; 3) feeding; 4) controlling a raising environment; and 5) timely sorting out the ill, disabled and weak perinereis aibuhitensis during the raising process. The indoor raising method for the perinereis aibuhitensis by circulating water provided by the invention is formed by improving through adopting a circulating water raising mode on the basis of the perinereis aibuhitensis seawater raising. Under the precondition of ensuring the vigor of the perinereis aibuhitensis, the survival time of the perinereis aibuhitensis is prolonged, and the raising density is increased.
Description
Technical field
The present invention relates to a kind of method of storing up of perinereis aibihitensis Grube, particularly at the indoor technology of the storing up cultivation perinereis aibihitensis Grube that utilizes cycle water, belong to the mariculture technical field.
Background technology
Perinereis aibihitensis Grube (
Perinereis aibuhitensis) belong to Annelida, polychaeta, Nereidae, happiness is lived in caves in calm, nutritious silt particle beach, intertidal zone, is the sociales of silt beach, China intertidal zone biology, also is that the main composition of CHINESE OFFSHORE bottom community is biological, has the important ecological value.
Clam worm is the high-quality bait of fish, shrimp, crab etc., and nutritive value is abundant, is called as " omnipotent bait ", has high economic worth.The C.I.F. of Japanese market is per ton up to tens thousand of dollars at present, and very worthy equally in market, West Europe, supply falls short of demand.
Perinereis aibihitensis Grube has the characteristics such as individuality is large, protein content high (70%-80%), is the important kind that can develop in the Nereidae animal, has wide development prospect.In recent years, domestic existing many enterprises and institute have carried out the cultural technique research of perinereis aibihitensis Grube in succession.It is pool cultivated that main breeding way has mudflat aquaculture, the cultivation of batch production cement pit and shrimp at present.These three kinds of modes all survive in clam worm in " mud bank " environment.When clam worm reaches commercial specification (approximately 200/kg), need be excavated out from " mud bank ".The clam worm that lives is transported to the dealer place, often need to store up it in briny environment, to prolong the clam worm time-to-live.The technology of storing up of using at present only can make clam worm survival about 7 days, and a large amount of clam worms are dead because of the change of environmental condition.The sales cycle that this has just limited clam worm alive, increased the business risk of clam worm alive distributors.Utilize simultaneously the present technology of storing up, because the clam worm time-to-live is short, the correlative study that has limited take perinereis aibihitensis Grube as experiment sample is carried out.
Summary of the invention
For the defective that exists in the prior art, the object of the present invention is to provide a kind of efficiently, indoor circulating water is stored up the perinereis aibihitensis Grube technology easily.
For reaching above purpose, the technical scheme that the present invention takes is:
A kind of perinereis aibihitensis Grube indoor circulating water is stored up method, adopts following steps:
1) stores up preparation
In storing up case, the injection salinity is 26 the water of storing up, height of water level 40cm;
2) water, oxygen supply are stored up in circulation
Store up water with the water circulating pump circulation, the controlled circulation water volume flow rate is about 0.005m
3/ h, per hour rate of water exchange is about 25%; Use aeration pump, keep and store up that dissolved oxygen content is always more than the 10mg/L in the water body;
2) put in a suitable place to breed
The density of storing up according to 100/L is put perinereis aibihitensis Grube in a suitable place to breed storing up in the water in storing up case;
3) bait throwing in
The Powdered shrimp pellet that input is filtered through 60 order mesh screens, protein content 30%～35%, the frequency of throwing something and feeding is to throw something and feed once in per two days, the single feeding volume is the 4g/100 bar;
4) culture environment control
It is 15 ± 1 ℃ that temperature is stored up in control; Illumination is about 1000 μ molm
-2S
-1, light dark period is than being 12:12;
5) store up sick, residual, the weak perinereis aibihitensis Grube of timely cleaning in the process.
On the basis of such scheme, the described case of storing up is the vinyon material.
On the basis of such scheme, the described case of storing up soaks 24h with 1% watery hydrochloric acid before use, uses afterwards the K of 20mg/L
2MnO
4Sterilization is cleaned with clear water at last.
On the basis of such scheme, the described water of storing up is that nature seawater and running water allotment form.
On the basis of such scheme, described nature seawater is that the fibrous filter membrane of 45 μ m filters with the aperture behind 7 days quiescent settings again, 121 ℃ afterwards, and the 20min sterilization treatment.
On the basis of such scheme, described running water was placed on the position of well-ventilated, illumination abundance, did not stop aeration through 7 days.
On the basis of such scheme, the described water of storing up prepares rear adding BZT(ammonium nitrate), making its concentration is 0.3PPM.
Illustrate: BZT is probiotics commonly used in the production of U.S. BIO-FormL.L.C. (Bi Wofeng biotech firm) company, the mariculture, and it is proportionally configured by microbial composite bacteria+biology enzyme and forms, and has the effect at the clear end of water purification.The BZT(ammonium nitrate) is a kind of in many BZT products, rapid nitrite and the ammonia nitrogen in the degrading cultivation water, the breeding growth of establishment noxious bacteria.
A kind of perinereis aibihitensis Grube indoor circulating water method of storing up of the present invention is to store up as the basis with the perinereis aibihitensis Grube seawater, forms through improveing with the cycle water mode of storing up.Improved and stored up density, it is normal that vigor keeps, and effectively prolongs perinereis aibihitensis Grube in the time of indoor culture, improved and supported temporarily density.
Embodiment
Method among the following embodiment if no special instructions, is conventional method.
Percentage composition among the following embodiment if no special instructions, is the quality percentage composition.
Embodiment 1
A kind of perinereis aibihitensis Grube indoor circulating water is stored up method, adopts following steps:
1) stores up preparation
In storing up case, the injection salinity is 26 the water of storing up, height of water level 40cm;
2) water, oxygen supply are stored up in circulation
Store up water with the water circulating pump circulation, the controlled circulation water volume flow rate is about 0.005m
3/ h, per hour rate of water exchange is about 25%; Use aeration pump, keep and store up that dissolved oxygen content is always more than the 10mg/L in the water body;
2) put in a suitable place to breed
The density of storing up according to 100/L is put perinereis aibihitensis Grube in a suitable place to breed storing up in the water in storing up case;
3) bait throwing in
The Powdered shrimp pellet that input is filtered through 60 order mesh screens, protein content 30%～35%, the frequency of throwing something and feeding is to throw something and feed once in per two days, the single feeding volume is the 4g/100 bar;
4) culture environment control
It is 15 ± 1 ℃ that temperature is stored up in control; Illumination is about 1000 μ molm
-2S
-1, light dark period is than being 12:12;
5) store up sick, residual, the weak perinereis aibihitensis Grube of timely cleaning in the process.
Preferably, the described case of storing up is the vinyon material.
Preferably, the described case of storing up soaks 24h with 1% watery hydrochloric acid before use, uses afterwards the K of 20mg/L
2MnO
4Sterilization is cleaned with clear water at last.
Preferably, the described water of storing up is that nature seawater and running water allotment form.
Preferably, described nature seawater is that the fibrous filter membrane of 45 μ m filters with the aperture behind 7 days quiescent settings again, 121 ℃ afterwards, and the 20min sterilization treatment.
Preferably, described running water was placed on the position of well-ventilated, illumination abundance, did not stop aeration through 7 days.
Preferably, the described water of storing up prepares rear adding BZT(ammonium nitrate), making its concentration is 0.3PPM.
Embodiment 2
According to the method among the embodiment 1, select the long 15-25cm of being of 500 bodies, body colour gloss, the normal healthy perinereis aibihitensis Grube of vigor is divided into 5 groups, 100 every group, stores up 30 days.Support temporarily the result as follows:
Table 1 perinereis aibihitensis Grube indoor circulating water is stored up 30 days results
| ? | Dead (bar) | Survival rate % |
| First group | 12 | 88% |
| Second group | 14 | 86% |
| The 3rd group | 15 | 85% |
| The 4th group | 18 | 82% |
| The 5th group | 12 | 88% |
Find through storing up after 30 days, the perinereis aibihitensis Grube survival rate of storing up with the method reaches more than 80%.
Claims (7)
1. a perinereis aibihitensis Grube indoor circulating water is stored up method, it is characterized in that adopting following steps:
1) stores up preparation
In storing up case, the injection salinity is 26 the water of storing up, height of water level 40cm;
2) water, oxygen supply are stored up in circulation
Store up water with the water circulating pump circulation, the controlled circulation water volume flow rate is about 0.005m
3/ h, per hour rate of water exchange is about 25%; Use aeration pump, keep and store up that dissolved oxygen content is always more than the 10mg/L in the water body;
2) put in a suitable place to breed
The density of storing up according to 100/L is put perinereis aibihitensis Grube in a suitable place to breed storing up in the water in storing up case;
3) bait throwing in
The Powdered shrimp pellet that input is filtered through 60 order mesh screens, protein content 30%～35%, the frequency of throwing something and feeding is to throw something and feed once in per two days, the single feeding volume is the 4g/100 bar;
4) culture environment control
It is 15 ± 1 ℃ that temperature is stored up in control; Illumination is about 1000 μ molm
-2S
-1, light dark period is than being 12:12;
5) store up sick, residual, the weak perinereis aibihitensis Grube of timely cleaning in the process.
2. perinereis aibihitensis Grube indoor circulating water according to claim 1 is stored up method, it is characterized in that the described case of storing up is the vinyon material.
3. perinereis aibihitensis Grube indoor circulating water according to claim 1 and 2 is stored up method, it is characterized in that the described case of storing up soaks 24h with 1% watery hydrochloric acid before use, uses the K of 20mg/L afterwards
2MnO
4Sterilization is cleaned with clear water at last.
4. perinereis aibihitensis Grube indoor circulating water according to claim 1 is stored up method, it is characterized in that the described water of storing up is that nature seawater and running water allotment form.
5. perinereis aibihitensis Grube indoor circulating water according to claim 4 is stored up method, it is characterized in that described nature seawater behind 7 days quiescent settings, is that the fibrous filter membrane of 45 μ m filters with the aperture again, and 121 ℃ afterwards, the 20min sterilization treatment.
6. perinereis aibihitensis Grube indoor circulating water according to claim 4 is stored up method, it is characterized in that described running water is placed on the position of well-ventilated, illumination abundance, does not stop aeration through 7 days.
7. perinereis aibihitensis Grube indoor circulating water according to claim 1 is stored up method, it is characterized in that the described water of storing up prepares rear adding BZT(ammonium nitrate), making its concentration is 0.3PPM.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201210577849.6A CN103004701B (en) | 2012-12-27 | 2012-12-27 | Indoor raising method for perinereis aibuhitensis by circulating water |
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| CN201210577849.6A CN103004701B (en) | 2012-12-27 | 2012-12-27 | Indoor raising method for perinereis aibuhitensis by circulating water |
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| CN103004701B CN103004701B (en) | 2014-07-30 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103283689A (en) * | 2013-05-23 | 2013-09-11 | 山东省海水养殖研究所 | Propagation control method for indoor cultivation of Perinereis aibuhitensis |
| CN103734093A (en) * | 2013-12-30 | 2014-04-23 | 盐城工学院 | Method for perinereis aibuhitensis heteronereis cultivation and synchronous collection |
| CN104126548A (en) * | 2014-07-17 | 2014-11-05 | 山东省海洋生物研究院 | Method for distributing perinereis aibuhitensis grube seedlings on large-area soil pit |
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| CN1802906A (en) * | 2005-11-25 | 2006-07-19 | 宁波大学 | Perinereis aibihitensis Grube manual seedling-raising method |
| CN1817121A (en) * | 2006-03-08 | 2006-08-16 | 何丽辉 | Clam worm culturing metod |
| CN101053315A (en) * | 2007-05-28 | 2007-10-17 | 大连水产学院 | Method for indoors artificial intensive culture of clamworm |
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| CN102349474A (en) * | 2011-08-22 | 2012-02-15 | 大连海洋大学 | Method for artificially culturing perineries aibuhitensis in biological filter |
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2012
- 2012-12-27 CN CN201210577849.6A patent/CN103004701B/en not_active Expired - Fee Related
Patent Citations (5)
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| CN1802906A (en) * | 2005-11-25 | 2006-07-19 | 宁波大学 | Perinereis aibihitensis Grube manual seedling-raising method |
| CN1817121A (en) * | 2006-03-08 | 2006-08-16 | 何丽辉 | Clam worm culturing metod |
| CN101053315A (en) * | 2007-05-28 | 2007-10-17 | 大连水产学院 | Method for indoors artificial intensive culture of clamworm |
| CN102106326A (en) * | 2011-03-02 | 2011-06-29 | 盐城工学院 | Method for three-dimensional and artificial seedlings cultivation of perinereis aibuhitensis |
| CN102349474A (en) * | 2011-08-22 | 2012-02-15 | 大连海洋大学 | Method for artificially culturing perineries aibuhitensis in biological filter |
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| 马悦欣等: "沙蚕闭合循环式养殖系统中细菌的数量及其代谢活性", 《大连水产学院学报》, vol. 20, no. 3, 30 September 2005 (2005-09-30) * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103283689A (en) * | 2013-05-23 | 2013-09-11 | 山东省海水养殖研究所 | Propagation control method for indoor cultivation of Perinereis aibuhitensis |
| CN103734093A (en) * | 2013-12-30 | 2014-04-23 | 盐城工学院 | Method for perinereis aibuhitensis heteronereis cultivation and synchronous collection |
| CN103734093B (en) * | 2013-12-30 | 2016-04-27 | 盐城工学院 | Perinereis aibihitensis Grube heteronereis is cultivated and synchronous collection method |
| CN104126548A (en) * | 2014-07-17 | 2014-11-05 | 山东省海洋生物研究院 | Method for distributing perinereis aibuhitensis grube seedlings on large-area soil pit |
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| CN103004701B (en) | 2014-07-30 |
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Address after: 266000 Shandong city of Qingdao province Guizhou City Road No. 47 Applicant after: Shandong Marine Biology Institute Address before: 266000 Shandong city of Qingdao province Guizhou City Road No. 47 Applicant before: Shandong Province Marine Culture Institute |
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Free format text: CORRECT: APPLICANT; FROM: SHANDONG PROVINCE MARINE CULTURE INSTITUTE TO: SHANDONG MARINE BIOLOGY INSTITUTE |
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