CN114631503A - Artificial seedling culture method for Babylonia - Google Patents
Artificial seedling culture method for Babylonia Download PDFInfo
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- CN114631503A CN114631503A CN202210181234.5A CN202210181234A CN114631503A CN 114631503 A CN114631503 A CN 114631503A CN 202210181234 A CN202210181234 A CN 202210181234A CN 114631503 A CN114631503 A CN 114631503A
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- 241000606434 Babylonia Species 0.000 title claims abstract description 51
- 238000012136 culture method Methods 0.000 title description 4
- 238000009395 breeding Methods 0.000 claims abstract description 48
- 150000001875 compounds Chemical class 0.000 claims abstract description 22
- 230000002708 enhancing effect Effects 0.000 claims abstract description 15
- 230000012447 hatching Effects 0.000 claims abstract description 10
- 238000012258 culturing Methods 0.000 claims abstract description 8
- 241000237858 Gastropoda Species 0.000 claims description 70
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 55
- 238000005728 strengthening Methods 0.000 claims description 37
- 239000000843 powder Substances 0.000 claims description 36
- 230000001488 breeding effect Effects 0.000 claims description 30
- 241000195493 Cryptophyta Species 0.000 claims description 19
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims description 18
- 241000196324 Embryophyta Species 0.000 claims description 18
- 239000007858 starting material Substances 0.000 claims description 17
- 230000033228 biological regulation Effects 0.000 claims description 14
- 235000017060 Arachis glabrata Nutrition 0.000 claims description 9
- 244000105624 Arachis hypogaea Species 0.000 claims description 9
- 235000010777 Arachis hypogaea Nutrition 0.000 claims description 9
- 235000018262 Arachis monticola Nutrition 0.000 claims description 9
- 241001474374 Blennius Species 0.000 claims description 9
- 241001597062 Channa argus Species 0.000 claims description 9
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 claims description 9
- 235000007164 Oryza sativa Nutrition 0.000 claims description 9
- 241001339782 Scapharca broughtonii Species 0.000 claims description 9
- 229930003268 Vitamin C Natural products 0.000 claims description 9
- 235000020232 peanut Nutrition 0.000 claims description 9
- 235000009566 rice Nutrition 0.000 claims description 9
- 210000001835 viscera Anatomy 0.000 claims description 9
- 235000019154 vitamin C Nutrition 0.000 claims description 9
- 239000011718 vitamin C Substances 0.000 claims description 9
- 241000830535 Ligustrum lucidum Species 0.000 claims description 8
- 235000013399 edible fruits Nutrition 0.000 claims description 8
- 241000237502 Ostreidae Species 0.000 claims description 7
- 235000013372 meat Nutrition 0.000 claims description 7
- 235000020636 oyster Nutrition 0.000 claims description 7
- 241000195649 Chlorella <Chlorellales> Species 0.000 claims description 6
- 238000002156 mixing Methods 0.000 claims description 6
- 239000002994 raw material Substances 0.000 claims description 6
- 239000004576 sand Substances 0.000 claims description 6
- 238000003756 stirring Methods 0.000 claims description 6
- 241000628923 Anadara sativa Species 0.000 claims description 5
- 238000011161 development Methods 0.000 claims description 5
- 230000018109 developmental process Effects 0.000 claims description 5
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- 230000029052 metamorphosis Effects 0.000 claims description 5
- 238000002791 soaking Methods 0.000 claims description 5
- ZNCPFRVNHGOPAG-UHFFFAOYSA-L sodium oxalate Chemical compound [Na+].[Na+].[O-]C(=O)C([O-])=O ZNCPFRVNHGOPAG-UHFFFAOYSA-L 0.000 claims description 5
- 229940039790 sodium oxalate Drugs 0.000 claims description 5
- 241000208689 Eucommia ulmoides Species 0.000 claims description 4
- 241001571764 Lysimachia christinae Species 0.000 claims description 4
- 239000002131 composite material Substances 0.000 claims description 3
- 241000195940 Bryophyta Species 0.000 claims description 2
- 241000969495 Hemisalanx Species 0.000 claims 1
- 241001123258 Meretrix meretrix Species 0.000 claims 1
- 240000007594 Oryza sativa Species 0.000 claims 1
- 241000196316 Tetraselmis subcordiformis Species 0.000 claims 1
- 238000010298 pulverizing process Methods 0.000 claims 1
- 235000013601 eggs Nutrition 0.000 abstract description 11
- 230000004083 survival effect Effects 0.000 abstract description 10
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- 238000000034 method Methods 0.000 description 13
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- 241001113556 Elodea Species 0.000 description 9
- 235000020639 clam Nutrition 0.000 description 9
- 241000209094 Oryza Species 0.000 description 8
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 8
- 229910052760 oxygen Inorganic materials 0.000 description 8
- 239000001301 oxygen Substances 0.000 description 8
- 241000237519 Bivalvia Species 0.000 description 7
- 241000606431 Babylonia lutosa Species 0.000 description 5
- 241001124553 Lepismatidae Species 0.000 description 5
- 241000196317 Platymonas Species 0.000 description 5
- 241000920062 Babylonia areolata Species 0.000 description 4
- 241000208688 Eucommia Species 0.000 description 4
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- 241001661641 Verrucosa Species 0.000 description 2
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- 241000894007 species Species 0.000 description 2
- 240000002900 Arthrospira platensis Species 0.000 description 1
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- 206010012218 Delirium Diseases 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/50—Culture of aquatic animals of shellfish
- A01K61/51—Culture of aquatic animals of shellfish of gastropods, e.g. abalones or turban snails
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/003—Aquaria; Terraria
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/22—Animal feeding-stuffs from material of animal origin from fish
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/26—Animal feeding-stuffs from material of animal origin from waste material, e.g. feathers, bones or skin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention provides an artificial breeding method of Babylonia, which comprises the steps of enhancing, hatching and culturing Babylonia larvae, feeding the Babylonia larvae by combining fresh bait and compound Babylonia enhancing feed in a Babylonia enhancing stage, and regulating and enhancing by matching with light, so that the aims of stimulating the emission of seminal eggs of the Babylonia and improving the quality of the seminal eggs are fulfilled. The hatching rate of the artificial breeding method of Babylonia reaches 98.9 percent, and the survival rate of the larva reaches 97.8 percent.
Description
Technical Field
The invention relates to the field of Babylonia breeding, in particular to an artificial seedling method of Babylonia.
Background
Babylonia is commonly called "Hua-Lu", Hai-Lu "and" nan-Lu ". The meat is delicious, crisp and tasty, and is high-quality marine shellfish which is very popular in the market at home and abroad. The main species in China are three species of Babylonia, Babylonia and Taiwan Babylonia. The yield of the mud Babylonia in the natural sea area is limited, so that the vast market demand cannot be met. At present, the babylonia lutosa is bred in an artificial farm gradually in a large scale, but the larger problem exists that the bottom quality of the babylonia lutosa is easy to change black and deteriorate in the process of breeding the babylonia lutosa, the growth environment of the babylonia lutosa is damaged, the growth of the babylonia lutosa is slow, and even more, the phenomenon of large-scale death occurs. CN 201610184641.6: the method for breeding the Babylonia in mud further improves the yield of the Babylonia by laying a breeding pond; CN 201911278733.0: an artificial breeding method for mud dongfeng snail includes such steps as preparing fermented fish paste from small fish, brown sugar and distiller's yeast, culturing the algae and rotifer in natural seawater by the aid of treated human urine to obtain initial feed, culturing the young snail by feeding fresh oyster paste, and classifying. The invention provides a method for improving the quality of Babylonia during the culture of Babylonia by enhancing the quality of Babylonia eggs and the quality of Babylonia larvae by combining the hatching method of the invention.
Disclosure of Invention
Therefore, the invention provides an artificial breeding method of Babylonia, which can improve the hatchability of fertilized eggs and shorten the artificial breeding time.
The technical scheme of the invention is realized as follows:
an artificial breeding method of Babylonia includes the following steps:
s1: enhancing parent spiral shell: and putting the breeding snails into a strengthening pond, putting baits twice a day, wherein the baits consist of 1:3-5 of fresh baits and composite breeding snail strengthening feedstuff, and culturing for 7-9 days by combining light regulation and control.
And S2, mating and spawning the strengthened breeding snails, collecting spawns, soaking the spawns in 1-3% sodium oxalate solution for 1-3min, and putting the spawns into a net cage for hatching for 4-6 days.
S3: transferring the hatched larva to a culture pond for culture, observing the development of the larva after 8-12 days of culture, putting open feed when the larva begins metamorphosis development, and culturing for 24-26 days to obtain the snail seedling.
Further, in step S1, the fresh baits are the small horse hoof snail and the purple blood clam with the mass ratio of 1: 1-3.
Further, in step S1, the bait is added in an amount of 0.45-0.55 times the weight of the parent snails.
Further, in step S1, the compound snail feed comprises, by weight, 10-12 parts of peanut powder, 3-5 parts of rice bran, 2-4 parts of seaweed powder, 7-9 parts of snakehead viscera powder, 5-8 parts of arca subcrenata powder, 4-7 parts of vitamin C, 4-7 parts of eucommia ulmoides, 1-3 parts of glossy privet fruit, and 1-3 parts of lysimachia christinae.
Further, in step S1, the lighting is controlled to be irradiated by yellow light with intensity of 1400-.
Further, in step S3, the cultivation pond is formed by paving 3-5cm of fine sand at the bottom of the pond, planting water plants on the periphery of the pond, wherein the pond water depth is 50-70cm, the water surface is inoculated with algae plants of chlorella, goldfish algae and Platymonas subcontratus in a mass ratio of 1:1:3, and the coverage rate of the algae plants is 50-60%.
Further, in step S3, the starter feed is prepared from oysters, clams and silverfish in a mass ratio of 2-3:5-7:8-10, and the starter feed is prepared by mixing, stirring and crushing meat serving as raw materials.
Further, in step S3, the open feed is added in an amount of 0.1 to 0.3 times the weight of the larvae for 1 to 5 days, and the amount of the open feed added is 0.5 to 0.7 times the weight of the larvae on the sixth day.
Furthermore, the water temperature of the culture is 20-40 ℃, the dissolved oxygen in the water body is 7-9mg/L, the salinity is 25-35 per mill, and the pH value is 7.5-8.5.
Compared with the prior art, the invention has the beneficial effects that:
according to the artificial breeding method of the Babylonia, the quality of Babylonia sperm eggs is improved through composite Babylonia strengthening feed and water temperature regulation in the Babylonia strengthening process, the hatchability and the survival rate of seedlings are further improved, and meanwhile, the temperature resistance of the Babylonia seedlings can be improved. The compound feed for enhancing the breeding snails can promote the growth of the breeding snails and the discharge of sperm and eggs. According to the invention, through the arrangement of the culture pond, the water quality is stabilized, the growth of Babylonia larvae is promoted, the variation coefficient of the Babylonia larvae is reduced, the color depth of the shell patterns of the Babylonia is moderate, and the color of the patterns in batches has no obvious difference. The young snails with the body length of 1cm are harvested 25 days after the artificial breeding method of Babylonia, the hatchability is as high as 98.9 percent, and the survival rate of the young snails is as high as 97.8 percent.
Detailed Description
In order to better understand the technical content of the invention, specific examples are provided below to further illustrate the invention.
The experimental methods used in the examples of the present invention are all conventional methods unless otherwise specified.
The materials, reagents and the like used in the examples of the present invention are commercially available unless otherwise specified.
Example 1 Artificial seedling method of Babylonia
(1) Enhancing parent spiral shell: and (3) throwing the breeding snails into a strengthening pond, and throwing baits twice a day, wherein the throwing time is 8:30 in the morning and 4 in the afternoon: 30, the bait consists of fresh bait and compound snail strengthening feed in a mass ratio of 1:4, the bait dosage is 0.5 times of the weight of parent snails each time, the fresh bait is a small water leg snail and a purple blood clam with a mass ratio of 1:2, the compound seed snail strengthening feed consists of 11 parts of peanut powder, 4 parts of rice bran, 3 parts of seaweed powder, 8 parts of snakehead viscera powder, 6.5 parts of blood clam powder, 5.5 parts of vitamin C, 5.5 parts of eucommia bark, 2 parts of glossy privet fruit and 2 parts of longhairy antenoron herb by weight, the fresh bait is cultivated for 8 days by combining light regulation and control, the lamplight is controlled to be irradiated by yellow light with the intensity of 1500lx at 8-10 am, and by yellow light with the intensity of 1100lx at 4-5 pm, the residual bait is cleaned and 10% of water in the strengthening tank is replaced every morning, the water temperature of the culture is 30 ℃, the dissolved oxygen in the water body is 8mg/L, the salinity is 30 per mill, and the pH value is 8.
(3) Mating and spawning the strengthened breeding snails, collecting spawns, soaking the spawns in a sodium oxalate solution with the mass concentration of 2% for 2min, and then putting the spawns into a net cage for incubation for 5 days.
(3) Transferring the hatched larva to a culture pond for culture, wherein the culture pond is formed by paving 4cm of fine sand at the bottom of the pond and planting water plants around the pond, the waterweeds are waterweeds, the water depth of the pond is 70cm, the water surface is inoculated with algae plants of chlorella, hornworts and platymonas in a mass ratio of 1:1:3, the coverage rate of the algae plants is 55%, after 10 days of cultivation, the growth of larvae is observed, when the larvae start to develop in a metamorphosis mode, starter feed is put in, the starter feed is prepared from oysters, clams and silverfish with the mass ratio of 2.5:6:9, the starter is prepared by mixing, stirring and crushing meat serving as raw materials, wherein the feeding amount of the starter is 1-5 days, 0.2 time of the weight of a larva is fed, the feeding amount of the starter is 0.6 time of the weight of the larva at the sixth day, the residual bait is cleaned in the early morning every day, water is replaced once every two days, the water replacement amount is one third, and the snail seedlings can be harvested after 25 days of cultivation.
Example 2 Artificial rearing method of Babylonia
(1) Enhancing parent spiral shell: and (3) throwing the breeding snails into a strengthening pond, and throwing baits twice a day, wherein the throwing time is 8:30 in the morning and 4 in the afternoon: 30, the bait consists of fresh bait and compound snail strengthening feed in a mass ratio of 1:3, the bait dosage is 0.5 times of the weight of parent snails each time, the fresh bait is a small water leg snail and a bloody clam with a mass ratio of 1:1, the compound snail-breeding reinforced feed consists of 10 parts of peanut powder, 3 parts of rice bran, 2 parts of seaweed powder, 7 parts of snakehead viscera powder, 5 parts of blood clam powder, 4 parts of vitamin C, 4 parts of eucommia bark, 1 part of glossy privet fruit and 1 part of desmodium by weight, and is cultivated for 7 days by combining light regulation and control, the light is controlled to be irradiated by yellow light with the intensity of 1400lx at 8-10 am, by yellow light with the intensity of 1000lx at 4-5 pm, the residual bait is cleaned and 10% of water in the strengthening pond is replaced every morning, the culture water temperature is 20 ℃, the dissolved oxygen in the water body is 7mg/L, the salinity is 25 per thousand, and the pH value is 7.5.
(2) Mating and spawning the strengthened breeding snails, collecting spawns, soaking the spawns in a sodium oxalate solution with the mass concentration of 1% for 1min, and then putting the spawns into a net cage for hatching for 4 days.
(3) Transferring the hatched larva to a culture pond for culture, wherein the bottom of the culture pond is paved with 3cm of fine sand, waterweeds are planted around the culture pond, the waterweeds are waterweeds, the water depth of the pond is 50cm, the water surface is inoculated with algae plants of chlorella, goldfish algae and Platymonas verrucosa in a mass ratio of 1:1:3, the coverage rate of the algae plants is 50%, after the algae plants are cultivated for 8-12 days, the growth of larvae is observed, when the larvae begin to metamorphose and develop, the starter feed is put in, the starter feed is prepared from oysters, clams and silverfish in a mass ratio of 2:5:8, is prepared by mixing, stirring and crushing meat serving as raw materials, the putting amount of the open feed is 1-5 days, 0.1 time of the weight of the larva is put in the open feed, the putting amount of the open feed is 0.5 time of the weight of the larva from the sixth day, the residual feed is cleaned in the early morning every day, water is replaced once every two days, the water replacement amount is one third, and the snail seedlings can be harvested after 25 days of cultivation.
Example 3 Artificial seedling method of Babylonia
(1) Enhancing parent spiral shell: and (3) throwing the breeding snails into a strengthening pond, and throwing baits twice a day, wherein the throwing time is 8:30 in the morning and 4 in the afternoon: 30, the bait consists of fresh bait and compound snail strengthening feed in a mass ratio of 1:5, the bait feeding amount is 0.5 times of the weight of parent snails each time, the fresh bait consists of small horse hoof snails and purple clams in a mass ratio of 1:3, the compound snail strengthening feed consists of 12 parts of peanut powder, 5 parts of rice bran, 4 parts of seaweed powder, 9 parts of snakehead viscera powder, 8 parts of scapharca subcrenata powder, 7 parts of vitamin C, 7 parts of eucommia ulmoides, 3 parts of glossy privet fruits and 3 parts of lysimachia christinae hance in parts by weight, the compound snail strengthening feed is cultivated for 9 days by combining light regulation and control, the light regulation and control are that yellow light with the intensity of 1400-1600lx is adopted at 8-10 morning, yellow light irradiation with the intensity of 1000-1200lx is adopted at 4-5 afternoon, 10% of water in a residual bait and strengthening pond is cleaned every morning, the cultivation temperature is 40 ℃, the dissolved oxygen in water is 9mg/L, and the salinity is 35 per mill, the pH was 8.5.
(2) Mating and spawning the strengthened breeding snails, collecting spawns, soaking the spawns in 3% sodium oxalate solution for 3min, and then putting the spawns into a net cage for 6 days.
(3) Transferring the hatched larva to a culture pond for culture, wherein the culture pond is formed by paving 5cm of fine sand at the bottom of the pond and planting water plants around the pond, the waterweeds are waterweeds, the water depth of the pond is 70cm, the water surface is inoculated with algae plants of chlorella, goldfish algae and Platymonas verrucosa in a mass ratio of 1:1:3, the coverage rate of the algae plants is 60 percent, after the cultivation for 8 to 12 days, the development of larvae is observed, when the larvae begin to develop in a metamorphosis state, the initial feed is put in, the starter feed is prepared from oysters, clams and silverfish in a mass ratio of 3:7:10, is prepared by mixing, stirring and crushing meat serving as raw materials, the putting amount of the open feed is 1-5 days, 0.3 time of the weight of the larva is put in the open feed, the putting amount of the open feed is 0.7 time of the weight of the larva from the sixth day, the residual feed is cleaned in the early morning every day, water is replaced once every two days, the water replacement amount is one 6, and the snail seedlings can be harvested after 25 days of cultivation.
Example 4 Artificial seedling method of Babylonia
On the basis of the embodiment 1, in the step (1), the strengthening is performed without adopting a light regulation method, specifically: enhancing parent spiral shell: and (3) throwing the breeding snails into a strengthening pond, and throwing baits twice a day, wherein the throwing time is 8:30 in the morning and 4 in the afternoon: 30, the bait consists of fresh bait and compound snail strengthening feed in a mass ratio of 1:4, the bait put amount is 0.5 times of the weight of the parent snail each time, the fresh bait consists of small horse hoof snails and red blood clams in a mass ratio of 1:2, the compound snail strengthening feed consists of 11 parts of peanut powder, 4 parts of rice bran, 3 parts of seaweed powder, 8 parts of snakehead viscera powder, 6.5 parts of scapharca subcrenata powder, 5.5 parts of vitamin C, 5.5 parts of eucommia ulmoides, 2 parts of glossy privet fruit and 2 parts of lysimachia christinae by weight, the residual bait is cleaned and 10% of water in a strengthening pond is replaced every morning, the cultivation temperature is 30 ℃, the dissolved oxygen in water is 8mg/L, the water temperature is 30% per mill, and the pH value is 8.
Example 5 Artificial seedling method of Babylonia
On the basis of the embodiment 1, in the step (3), the arrangement of the seedling raising ponds is changed, and the specific steps are as follows: transferring the hatched larva to a culture pond for culture, wherein the culture pond is formed by paving 4cm of fine sand at the bottom of the pond and planting water plants around the pond, the waterweeds are waterweeds, the water depth of the pond is 70cm, algae plants of chlorella, spirulina and platymonas are inoculated on the water surface according to the mass ratio of 1:1:3, the coverage rate of the algae plants is 75%, after 10 days of cultivation, the growth of larvae is observed, when the larvae start to develop in a metamorphosis mode, starter feed is put in, the starter feed is prepared from oysters, clams and silverfish with the mass ratio of 2.5:6:9, the starter is prepared by mixing, stirring and crushing meat serving as raw materials, wherein the feeding amount of the starter is 1-5 days, 0.2 time of the weight of a larva is fed, the feeding amount of the starter is 0.6 time of the weight of the larva at the sixth day, the residual bait is cleaned in the early morning every day, water is replaced once every two days, the water replacement amount is one third, and the snail seedlings can be harvested after 25 days of cultivation.
Comparative example 1
On the basis of the embodiment 1, in the step (1), the component ratio of the compound snail strengthening feed is adjusted, and the specific steps are as follows: enhancing parent spiral shell: and (3) throwing the breeding snails into a strengthening pond, and throwing baits twice a day, wherein the throwing time is 8:30 in the morning and 4 in the afternoon: 30, the bait consists of fresh bait and compound snail strengthening feed in a mass ratio of 1:4, the bait dosage is 0.5 times of the weight of parent snails each time, the fresh bait is a small water leg snail and a purple clam with a mass ratio of 1:2, the compound snail strengthening feed consists of 8 parts of peanut powder, 3 parts of rice bran, 6 parts of seaweed powder, 5 parts of snakehead viscera powder, 10 parts of blood clam powder, 8 parts of vitamin C, 3 parts of eucommia bark, 3 parts of glossy privet fruit and 3 parts of longhairy antenoron herb by weight, and is cultivated for 8 days by combining light regulation and control, the lamplight is controlled to be irradiated by yellow light with the intensity of 1500lx at 8-10 am, and by yellow light with the intensity of 1100lx at 4-5 pm, the residual bait is cleaned and 10% of water in the strengthening tank is replaced every morning, the water temperature of the culture is 30 ℃, the dissolved oxygen in the water body is 8mg/L, the salinity is 30 per mill, and the pH value is 8.
Comparative example 2
On the basis of the embodiment 1, in the step (1), the components of the compound snail-breeding reinforced feed are adjusted, specifically: enhancing parent spiral shell: and (3) throwing the breeding snails into a strengthening pond, and throwing baits twice a day, wherein the throwing time is 8:30 in the morning and 4 in the afternoon: 30, the bait consists of fresh bait and compound seed snail strengthening feed in a ratio of 1:4, the bait is 0.5 times of the weight of the parent snails each time, the fresh bait consists of small horse hoof snails and purple clams in a mass ratio of 1:2, the compound seed snail strengthening feed consists of 11 parts of peanut powder, 4 parts of rice bran, 3 parts of seaweed powder, 8 parts of snakehead viscera powder, 6.5 parts of scapharca subcrenata powder and 5.5 parts of vitamin C in parts by weight, the compound seed snail strengthening feed is cultivated for 8 days by combining light regulation and control, the light regulation and control are that yellow light with the intensity of 1500lx is adopted at 8-10 points in the morning, yellow light with the intensity of 1100lx is adopted at 4-5 points in the afternoon, the residual bait is cleaned and 10% of water in a strengthening pond is replaced every morning, the cultivation water temperature is 30 ℃, the dissolved oxygen in the water body is 8mg/L, the salinity is 30 per thousand, and the pH value is 8.
Comparative example 3
On the basis of embodiment 1, in step (1), the light irradiation intensity is adjusted, specifically: enhancing parent spiral shell: and (3) throwing the breeding snails into a strengthening pond, and throwing baits twice a day, wherein the throwing time is 8:30 in the morning and 4 in the afternoon: 30, the bait consists of fresh bait and compound snail strengthening feed in a mass ratio of 1:4, the bait dosage is 0.5 time of the weight of the parent snail each time, the fresh bait is a small water leg snail and a purple blood clam with a mass ratio of 1:2, the compound seed snail strengthening feed consists of 11 parts of peanut powder, 4 parts of rice bran, 3 parts of seaweed powder, 8 parts of snakehead viscera powder, 6.5 parts of blood clam powder, 5.5 parts of vitamin C, 5.5 parts of eucommia bark, 2 parts of glossy privet fruit and 2 parts of longhairy antenoron herb by weight, the fresh bait is cultivated for 8 days by combining light regulation and control, the light regulation and control are that yellow light with the intensity of 2000lx is adopted for irradiation at 8-10 am, yellow light with the intensity of 1500lx is adopted for irradiation at 4-5 pm, the residual bait is cleaned and 10% of water in the strengthening pond is replaced every morning, the water temperature of the culture is 30 ℃, the dissolved oxygen in the water body is 8mg/L, the salinity is 30 per mill, and the pH value is 8.
Test example 1
According to the method, the Babylonia areolata is cultured by taking Babylonia areolata as a breeding snail, a culture base is located in delirium in Hainan, the number of square meters in a culture pond is 20, the throwing amount of the Babylonia areolata larvae is 1400 per square meter, the culture methods of the examples 1-5 and the comparative examples 1-3 are adopted for culturing, and the hatching rate and the survival rate of the larvae are counted to evaluate the technical effect of the Babylonia areolata culture method.
Hatching rate is fertilized egg seedling number/fertilized egg total number multiplied by 100%
The survival rate of the larva is equal to the number of the snail seedlings/the total number of the put larvae multiplied by 100 percent
| Group of | Hatching rate (%) | Survival rate of larvae (%) |
| Example 1 | 98.9 | 97.8 |
| Example 2 | 98.4 | 97.5 |
| Example 3 | 98.6 | 97.4 |
| Example 4 | 86.2 | 91.6 |
| Example 5 | 91.5 | 92.8 |
| Comparative example 1 | 93.4 | 94.1 |
| Comparative example 2 | 90.9 | 93.2 |
| Comparative example 3 | 95.1 | 95.7 |
Experimental results show that the Babylonia breeding method provided by the invention not only has the effect of promoting fertilized egg hatching, but also can improve the survival rate of larvae, the lengths of the snails obtained by breeding are all more than 1cm, and the patterns and colors of the snails in batches of 1 to 3 in examples are similar and have no obvious difference. Example 4 and respectively, the incubation rate and the survival rate of the larvae are reduced because the light intensity is not regulated and adjusted by using light, the reasonable light regulation and control of the invention can promote the growth of the breeding snails, improve the sperm and egg activity of the breeding snails, and the water temperature can be regulated and controlled by the reasonable light intensity; embodiment 5, the arrangement of the seedling raising pond is adjusted, the algae is reasonably matched, the growth of the Babylonia larvae is more suitable, the water quality can be regulated and controlled through reasonable algae coverage, and the purpose of promoting the growth of the Babylonia larvae is also achieved; the proportion and the components of the snail breeding reinforced feed are adjusted in the comparative example 1 and the comparative example 2, so that the quality of the snail breeding eggs is reduced, and the hatchability and the survival rate of the larvae are reduced; comparative example 3 the light intensity was adjusted, resulting in a decrease in hatchability and survival rate of larvae.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (8)
1. An artificial breeding method of Babylonia is characterized by comprising the following steps:
s1: enhancing parent spiral shell: putting the breeding snails into a strengthening pond, putting baits twice a day, wherein the baits consist of fresh baits and composite breeding snail strengthening feedstuff with the mass ratio of 1:3-5, and culturing for 7-9 days by combining light regulation and control;
s2, mating and spawning the strengthened breeding snails, collecting spawns, soaking the spawns in a sodium oxalate solution with the mass concentration of 1-3% for 1-3min, and putting the spawns into a net cage for hatching for 4-6 days;
s3: transferring the hatched larva to a culture pond for culture, observing the development of the larva after 8-12 days of culture, putting open feed when the larva begins metamorphosis development, and culturing for 24-26 days to obtain the snail seedling.
2. The artificial breeding method of Babylonia according to claim 1, wherein in step S1, the fresh baits are small Martha' S snail and purple blood clam in a mass ratio of 1: 1-3.
3. The artificial breeding method of Babylonia as claimed in claim 1, wherein in step S1, the bait is put in an amount of 0.45-0.55 times the weight of parent Babylonia.
4. The artificial breeding method of Babylonia as claimed in claim 1, wherein in step S1, the compound Babylonia enhanced feed comprises, by weight, 10-12 parts of peanut powder, 3-5 parts of rice bran, 2-4 parts of seaweed powder, 7-9 parts of snakehead viscera powder, 5-8 parts of arca subcrenata powder, 4-7 parts of vitamin C, 4-7 parts of eucommia ulmoides, 1-3 parts of glossy privet fruit and 1-3 parts of lysimachia christinae hance.
5. The artificial breeding method of Babylonia as claimed in claim 1, wherein in step S1, the light is controlled to be irradiated by yellow light with intensity of 1400-1600lx at 8-10 am and by yellow light with intensity of 1000-1200lx at 4-5 pm.
6. The artificial breeding method of Babylonia as claimed in claim 1, wherein in step S3, the breeding pond is formed by laying 3-5cm of fine sand at the bottom of the pond, planting water plants around the pond with a water depth of 50-70cm, inoculating algae plants of chlorella, hornworts and Platymonas subcordiformis at a mass ratio of 1:1:3 on the water surface, and the coverage rate of the algae plants is 50-60%.
7. The artificial breeding method of Babylonia as claimed in claim 1, wherein in step S3, the starter feed is prepared from oyster, Meretrix meretrix Linnaeus and Hemisalanx in a mass ratio of 2-3:5-7:8-10, and the starter feed is prepared by mixing, stirring and pulverizing meat as raw materials.
8. The artificial breeding method of Babylonia as claimed in claim 1, wherein in step S3, the open feed is added in an amount of 0.1-0.3 times the weight of the larvae for 1-5 days, and in an amount of 0.5-0.7 times the weight of the larvae after the sixth day.
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