CN111374076A - Penaeus vannamei feeding method - Google Patents

Penaeus vannamei feeding method Download PDF

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CN111374076A
CN111374076A CN202010387672.8A CN202010387672A CN111374076A CN 111374076 A CN111374076 A CN 111374076A CN 202010387672 A CN202010387672 A CN 202010387672A CN 111374076 A CN111374076 A CN 111374076A
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penaeus vannamei
feeding
vannamei boone
daphnia
compound feed
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何文辉
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Shanghai Kaitai Fish Culture Development Co ltd
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00Culture of aquatic animals
    • A01K61/50Culture of aquatic animals of shellfish
    • A01K61/59Culture of aquatic animals of shellfish of crustaceans, e.g. lobsters or shrimps
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/20Animal feeding-stuffs from material of animal origin
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/20Animal feeding-stuffs from material of animal origin
    • A23K10/22Animal feeding-stuffs from material of animal origin from fish
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/80Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

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Abstract

The invention belongs to the technical field of aquaculture, and particularly relates to a feeding method for penaeus vannamei boone. The method is characterized in that in the process of culturing the penaeus vannamei boone, the daphnia is adopted as feeding bait or one of the feeding baits. The penaeus vannamei bred by the special feeding method of the invention has larger specification than that of the common bred penaeus vannamei, and the weight of each tail is more than 3 times of that of the common bred penaeus vannamei. Moreover, special baits and feeding methods are adopted, so that the yield of the prawns is comprehensively improved, and the yield per mu is increased by more than 1000 kg; the quality of the prawn is optimized, the prawn body surface is smooth, the activity is good, no peculiar smell exists, the drug residue is qualified through inspection, the specification is neat, and the prawn body surface completely reaches the specification.

Description

Penaeus vannamei feeding method
Technical Field
The invention belongs to the technical field of aquaculture, and particularly relates to a feeding method for penaeus vannamei boone.
Background
Penaeus vannamei, known by the school name Penaeus vannamei. Is a wide-temperature and wide-salinity tropical shrimp, belongs to arthropoda, Crustacea, decapod and swimming sub-order, is native to the coastal water area of the Pacific ocean in south America, and is one of three well-recognized excellent shrimp species in the world. The appearance of the product is similar to that of Chinese prawn and black prawn, and the average service life can exceed 32 months at least. The longest length of the adult can reach 24cm, the carapace is thin, the normal body color is light grey, and no speckle exists in the whole body. The foot is usually chalk-like, so it is called white shrimp. The thin shell fertilizer of the south America white prawn shell has delicious meat quality, high meat content and rich nutrition.
Since the introduction of the last 90 s, the culture of the penaeus vannamei boone is rapidly developed, and the culture scale and the yield are continuously increased. Particularly, in recent years, with the continuous improvement of culture technology and supporting facilities, the intensification degree of the culture of the penaeus vannamei boone is greatly improved. According to the statistics of the fishery agency of the national Ministry of agriculture, in 2007, the yield of the Chinese seawater-cultured shrimps is 70.99 ten thousand tons, which accounts for 37.6 percent of the total amount of the Chinese cultured shrimps, wherein the yield of the south American white shrimps is 50.99 thousand tons, and accounts for 71.82 percent of the total proportion of the seawater-cultured shrimps. The yield of the Chinese seawater culture prawns in 2011 is 89.5 million tons, wherein the yield of the south America white prawns accounts for 73 percent, the export quantity is 21.6 million tons, the export amount accounts for 18.87 hundred million dollars, and the export amount accounts for 10.6 percent of the export amount of the cultured aquatic products, so that the first large variety of the aquatic products is formed. The main production area of the Chinese mariculture penaeus vannamei boone is in southern China, Guangdong, Guangxi and Hainan provinces in coastal areas.
The penaeus vannamei boone cannot resist low temperature, stops growing when the water temperature is lower than 15 ℃, and only lasts for about 4 months in the middle and lower reaches of Yangtze river in China. Therefore, during ordinary culture, the penaeus vannamei boone has slow growth speed, small weight, long time for reaching the market standard and low yield.
Disclosure of Invention
In order to overcome the problems in the prior art, the invention aims to provide a feeding method capable of improving the growth speed, yield and meat quality of penaeus vannamei boone.
In order to achieve the above objects and other related objects, the present invention adopts the following technical solutions:
the invention provides a feeding method of penaeus vannamei boone, which is characterized in that daphnia is adopted as feeding bait or one of the feeding baits in the process of culturing the penaeus vannamei boone, and the feeding method comprises the following steps:
(1) in the early stage of cultivation, the body length of the penaeus vannamei boone is 1-5 centimeters, and feeding daphnia and special compound feed for the penaeus vannamei boone as bait;
(2) in the middle stage of cultivation, the body length of the penaeus vannamei boone is 5-8 centimeters, and special compound feed for daphnia, river snails and the penaeus vannamei boone is fed as bait;
(3) in the later period of cultivation, the body length of the penaeus vannamei boone is more than 8 centimeters, and the penaeus vannamei boone is fed with the special compound feed for the viviparidae and the penaeus vannamei boone as bait.
Preferably, the daphnia belongs to cladocera or copepods.
Preferably, the daphnia is a daphnia magna.
Preferably, the average individual size of the adult daphnia magna is more than 5.0 mm. Further preferably, the average individual size of the adult daphnia magna is 5.0-6.5 mm. Food particles with a maximum diameter of about 80-160um can be filtered.
The obtaining mode of the daphnia magna can be obtained through a market approach. Can also be obtained by domestication. The invention has no special requirement on the obtaining way, as long as the average individual size of the obtained adult daphnia magna is more than 5.0 mm.
Preferably, in the step (1), the total feeding amount of the baits is 1-2% of the total weight of the pond shrimps.
Preferably, in the step (1), the daphnia is taken as a main part and the special compound feed for penaeus vannamei boone is taken as an auxiliary part during feeding.
Further preferably, the weight part ratio of the daphnia and the special compound feed for penaeus vannamei boone is as follows: (90-95): (10-5).
Further preferably, the special compound feed for penaeus vannamei boone is a No. 0 feed.
Preferably, in the step (2), the total feeding amount of the baits is 2-3% of the total weight of the pond shrimps.
Preferably, in the step (2), the daphnia and river snail are mainly used and the special compound feed for penaeus vannamei boone is used as an auxiliary feed during feeding.
Further preferably, the ratio of the daphnia, the river snail and the special compound feed for penaeus vannamei boone in parts by weight is as follows: (45-50): (40-50): (10-5).
Further preferably, the special compound feed for penaeus vannamei boone is a No. 1 feed.
Preferably, in the step (3), the total feeding amount of the baits is 3-5% of the total weight of the pond shrimps.
Preferably, in the step (3), the compound feed special for the penaeus vannamei boone is taken as the main material and the river snail is taken as the auxiliary material during feeding.
Further preferably, the weight parts of the river snail and the special compound feed for the penaeus vannamei boone are as follows: (10-5): (90-95).
Further preferably, the special compound feed for penaeus vannamei boone is a No. 2 feed.
Compared with the prior art, the invention has the following beneficial effects:
the penaeus vannamei bred by the special feeding method of the invention has larger specification than that of the common bred penaeus vannamei, and the weight of each tail is more than 3 times of that of the common bred penaeus vannamei. Moreover, special baits and feeding methods are adopted, so that the yield of the prawns is comprehensively improved, and the yield per mu is increased by more than 1000 kg; the quality of the prawn is optimized, the prawn body surface is smooth, the activity is good, no peculiar smell exists, the drug residue is qualified through inspection, the specification is neat, and the prawn body surface completely reaches the specification.
Detailed Description
The daphnia magna can be obtained through a market approach. Can also be obtained by domestication. The method is not particularly limited to the obtaining mode of the daphnia magna.
For example, the daphnia magna can be obtained by adopting the domestication mode in the invention patent with the application number of 2015101455719, namely the domestication method and the application thereof. But is not limited to this manner of obtaining.
The conception of the invention is as follows: putting daphnia magna into a domestication pond, culturing for a period of time by adopting specific domestication foodstuff, taking the dominant species for repeated domestication, purification and rejuvenation, and finally domesticating the daphnia magna into the natural enemy of the blue algae. The domestication and improvement of the daphnia magna have three meanings: improving digestion absorption blue algae intestinal tract; improving the caliber of the ingestion blue algae block mass; and (4) fresh water improvement of the stability of the saline water population. Through domestication, the average individual size of the adult daphnia magna is 5.0-6.5 mm, the maximum individual body length is 6.7mm, the maximum weight can reach 8.8mg, and food particles with the maximum diameter of about 80-160um can be filtered and eaten.
Specifically, the daphnia magna is obtained by the following domestication method, and the method comprises the following steps:
(1) feeding domesticated food: putting daphnia magna into water in a domestication pond, and feeding domesticated foodstuff every day;
(2) screening: selecting dominant species from the daphnia magna fed with the domesticated foodstuff in the step (1), wherein the dominant species are daphnia magna which is excellent in growth vigor and relatively large in individual.
(3) Circularly and repeatedly adopting the step (1) and the step (2) to feed domesticated foodstuff and re-screen the daphnia magna selected in the step (2) for multiple times until the total days for feeding the domesticated foodstuff is at least 60 days;
(4) and (3) stabilizing: and (4) putting the daphnia magna obtained in the step (3) in a specific water body for more than 50 days, and feeding domesticated food every day to stabilize and rejuvenate the domesticated improved characteristics.
In the step (1), the water body in the domestication pond is a common water body. The water temperature of the domestication pool is controlled to be a temperature suitable for survival of daphnia magna, and the temperature range suitable for survival of daphnia magna is generally 4-30 ℃. Preferably 15-26 ℃.
In the step (1), the domestication density of the daphnia magna can reach 50 ten thousand per m at most3The insect loading amount is 2.5kg/m 3. The domestication effect cannot be influenced by low feeding density of the daphnia magna, and the feeding density of the daphnia magna can be generally controlled to be 30-40 ten thousand/m in consideration of economic benefit3. The amount of domesticated food put in each time is generally as follows: feeding for 3-5 times per day, wherein the feed is 20-40g per square meter. The feeding time is 7-15 days.
Preferably, the daphnia magna initially bred in the step (1) is an individual large and active healthy wild daphnia magna with the length of 2-4 mm.
In the step (2), the dominant species have good growth condition and strong adaptive capacity of blue algae water body, and the individual is generally between 3 and 6 mm. Generally, the daphnia magna accounting for 20-40% of the total amount of the daphnia magna to be selected can be selected as the dominant species.
In the step (3), the total number of times of the feeding and screening cycles in the steps (1) to (2) is generally at least 5 times.
In the step (4), the feeding concentration range of the daphnia magna is 30-50 ten thousand/m3Preferably 30 to 40 ten thousand/m3. The specific water body has pH value of 5-10, dissolved oxygen of 2-8mg/l, and temperature of 4 deg.C toWater body at 30 deg.c. The feeding amount of the domesticated foodstuff is maintained at 20-40 g/square meter every time, and the domesticated foodstuff is fed for 3-5 times every day. The stabilization time is more than 50 days, and can be 50-60 days.
The domestication foodstuff for domesticating daphnia magna comprises the following raw materials in parts by weight:
Figure BDA0002484399510000041
preferably, the cyanobacteria is Microcystis aeruginosa (Microcystis aeruginosa).
The honey has no specific requirement on variety, and various conventional honey can be adopted.
The preparation method of the domesticated food comprises the following steps:
1) beating fresh banana peel and fresh dendrobium officinale stem into paste;
2) activating yeast dry powder;
3) mixing activated yeast, pasty fresh banana peel and fresh stem of dendrobium officinale with other raw materials according to a ratio, and fermenting under a condition suitable for yeast fermentation to obtain the domesticated food.
In step 2), the yeast dry powder is activated by the prior art, and can be activated by a conventional method, such as glucose water activation. As specifically exemplified in the examples of the present invention, each 450g of yeast dry powder can be activated with 150 g of glucose and 10L of pure water at 35 ℃.
Preferably, in step 3), the fermentation conditions are as follows: fermenting at 35-38 deg.C for 1-10 hr under sealed condition.
By adopting the domestication method, the daphnia magna which can stably grow in the wild can be obtained. It takes algae in water as its main food source, has a eating volume equivalent to tens of times of its own algae per day, and digests and decomposes other algae into water and inorganic salts, so that the algae in water is greatly reduced and population dominance is lost.
The daphnia magna is used as the natural bait of the penaeus vannamei for the first time, and the daphnia magna can convert rich nutrients such as algae and organic particles in the water body into animal protein to be ingested by the penaeus vannamei.
Meanwhile, after the domesticated daphnia magna ingests and digests the blue algae, weak acid excrement can be generated, the pH value of the water body is effectively reduced, the growth and the propagation of the blue algae can be inhibited, and the health and the stability of a water body ecological system are promoted.
In addition, the domesticated daphnia magna is a wide-spread species as a large cladocera zooplankton, and risks such as foreign species invasion do not exist.
Before the present embodiments are further described, it is to be understood that the scope of the invention is not limited to the particular embodiments described below; it is also to be understood that the terminology used in the examples is for the purpose of describing particular embodiments only, and is not intended to limit the scope of the present invention. Test methods in which specific conditions are not specified in the following examples are generally carried out under conventional conditions or under conditions recommended by the respective manufacturers.
When numerical ranges are given in the examples, it is understood that both endpoints of each of the numerical ranges and any value therebetween can be selected unless the invention otherwise indicated. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. In addition to the specific methods, devices, and materials used in the examples, any methods, devices, and materials similar or equivalent to those described in the examples may be used in the practice of the invention in addition to the specific methods, devices, and materials used in the examples, in keeping with the knowledge of one skilled in the art and with the description of the invention.
Unless otherwise indicated, the experimental methods, detection methods, and preparation methods disclosed herein all employ techniques conventional in the art of molecular biology, biochemistry, chromatin structure and analysis, analytical chemistry, cell culture, recombinant DNA technology, and related arts. These techniques are well described in the literature, and may be found in particular in the study of the MOLECULAR CLONING, Sambrook et al: a LABORATORY MANUAL, Second edition, Cold Spring harbor LABORATORY Press, 1989and Third edition, 2001; ausubel et al, Current PROTOCOLS Inmolecular BIOLOGY, John Wiley & Sons, New York, 1987and periodic updates; the series METHODS IN ENZYMOLOGY, Academic Press, San Diego; wolffe, CHROMATINSTRUCUTURE AND FUNCTION, Third edition, Academic Press, San Diego, 1998; (iii) Methods Inenzymolygy, Vol.304, Chromatin (P.M. Wassarman and A.P.Wolffe, eds.), academic Press, San Diego, 1999; and METHODS IN MOLECULAR BIOLOGY, Vol.119, chromatography protocols (P.B.Becker, ed.) Humana Press, Totowa, 1999, etc.
Examples
(1) Preparation work: sterilizing the culture pond and tools; and (4) treating the culture water, and preparing according to the conventional culture standard of the penaeus vannamei boone.
(2) Selecting shrimp larvae: selecting high-quality young penaeus vannamei boone with the body length of about 0.8 cm-1.2 cm as a young penaeus vannamei boone, and observing with naked eyes, wherein the young penaeus vannamei boone is uniform in size, transparent in body color and strong in activity; the young shrimps need to be combined together and pointed forward, the tail fan needs to be completely opened, and the abdominal joint needs to be longer; the body surface of the shrimp larvae is clean and has no parasitic organisms and damage; the shrimp larvae have full intestines and stomach, the stomach is orange red, and the ratio of the width of the abdominal muscle to the width of the intestinal tract is more than 4: 1; most of the shrimp larvae are in a bottom-leaning state in a static state, and have a water-lifting phenomenon after being stimulated by water flow. The shrimp larvae adopted in the embodiment are selected from Guangzhou seawater nursery.
(3) Stocking the shrimp larvae: stocking the shrimp seedlings at a stocking density of 10-15 thousand pieces/mu.
(3) Controlling culture temperature, salinity and oxygen: in the culture process, the temperature is controlled to be 23-28 ℃. The salinity of the water is controlled to be 1-3 per mill. The dissolved oxygen in water is always maintained at 5mg/l or more.
(4) Culturing and feeding: feeding in a mode of uniformly splashing the whole pool. In the initial stage of cultivation, the total feeding amount of baits is 1-2% of the total weight of pond shrimps. The total feeding amount of the bait is 2-3% of the total weight of pond shrimps. In the later culture period, the total feeding amount of the baits is 3-5% of the total weight of the pond shrimps. The principle of feeding less frequently is adhered to, and the bait is thrown for 4-6 times every day. In the first month after seedling placing, the feeding times can be arranged 4 times in general, 6-7 points, 10-11 points, 15-16 points and 20-21 points per day. The bait feeding amount is increased along with the growth of the prawns, the feeding times can be increased, the feeding is performed for 6 times every day from 6 o 'clock to 22 o' clock, the feeding is performed for about once in three hours, and the feeding in the afternoon accounts for about 60% of the feeding amount in the whole day. The bait is increased or decreased according to the weather, temperature, water quality and the activity of the shrimps.
The specific feeding method and the weight part ratio when feeding the bait are detailed in table 1. Wherein the average individual size of the daphnia magna is 5.0-6.5 mm.
Figure BDA0002484399510000061
TABLE 1 feeding method
(5) Catching adult shrimps: the shrimp is collected according to the conventional method and supplied to the market.
The specific cultivation effect is shown in table 2.
TABLE 2 cultivation Effect
Method 1 Method 2 Method 3 Comparative example
Seedling density (ten thousand tails/mu) 10 12 15 15
Exit specification (g/tail)) 60 80 90 20
Survival rate (%) 92% 92.5% 95% 70%
Specific yield (kg/mu) 1000 1100 1200 500
The data in the table show that the acre yield of the grown shrimps in the culture area of the culture pond is higher than 1000kg, and the survival rate is also higher than 92%. No obvious diseases appear in the culture process; the weight of the finished shrimps discharged from the pond is 60-90 g, the surface of the shrimp body is smooth, the activity is good, no peculiar smell exists, the drug residue is qualified through inspection, the specification is neat, and the standard is completely reached.
While the invention has been described with respect to a preferred embodiment, it will be understood by those skilled in the art that the foregoing and other changes, omissions and deviations in the form and detail thereof may be made without departing from the scope of this invention. Those skilled in the art can make various changes, modifications and equivalent arrangements, which are equivalent to the embodiments of the present invention, without departing from the spirit and scope of the present invention, and which may be made by utilizing the techniques disclosed above; meanwhile, any changes, modifications and variations of the above-described embodiments, which are equivalent to those of the technical spirit of the present invention, are within the scope of the technical solution of the present invention.

Claims (13)

1. A feeding method for penaeus vannamei boone is to adopt daphnia as feeding bait or one of the feeding baits in the process of culturing the penaeus vannamei boone, and comprises the following steps:
(1) in the early stage of cultivation, the body length of the penaeus vannamei boone is 1-5 centimeters, and feeding daphnia and special compound feed for the penaeus vannamei boone as bait;
(2) in the middle stage of cultivation, the body length of the penaeus vannamei boone is 5-8 centimeters, and special compound feed for daphnia, river snails and the penaeus vannamei boone is fed as bait;
(3) in the later period of cultivation, the body length of the penaeus vannamei boone is more than 8 centimeters, and the penaeus vannamei boone is fed with the special compound feed for the viviparidae and the penaeus vannamei boone as bait.
2. The method according to claim 1, wherein the daphnia is of the cladocera or copepods.
3. The method according to claim 1, wherein the daphnia is a daphnia magna.
4. The method according to claim 3, wherein the average individual size of the adult daphnia magna is above 5.0 mm.
5. The method according to claim 1, wherein in the step (1), the daphnia magna is taken as a main part and the special compound feed for penaeus vannamei is taken as an auxiliary part during feeding.
6. The method according to claim 1, wherein in the step (1), the ratio of the parts by weight of the daphnia and the special compound feed for penaeus vannamei during feeding is as follows: (90-95): (10-5).
7. The method according to claim 1, wherein in the step (1), the special compound feed for penaeus vannamei boone is a No. 0 feed.
8. The method according to claim 1, wherein in the step (2), the daphnia and river snail are mainly used and the special compound feed for penaeus vannamei boone is used as an auxiliary feed during feeding.
9. The method according to claim 1, wherein in the step (2), the ratio of the parts by weight of the daphnia, the river snail and the special compound feed for penaeus vannamei boone is as follows: (45-50): (40-50): (10-5).
10. The method according to claim 1, wherein in the step (2), the special compound feed for penaeus vannamei boone is a # 1 feed.
11. The method according to claim 1, wherein in the step (3), the compound feed special for the penaeus vannamei boone is mainly used and the river snail is used as an auxiliary feed during feeding.
12. The method according to claim 1, wherein in the step (3), the weight parts of the river snail and the special compound feed for penaeus vannamei boone are as follows: (10-5): (90-95).
13. The method according to claim 1, wherein in the step (3), the special compound feed for penaeus vannamei boone is a # 2 feed.
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