CN102742524A - Fry breeding method for thais luteostoma - Google Patents

Fry breeding method for thais luteostoma Download PDF

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Publication number
CN102742524A
CN102742524A CN2012101185932A CN201210118593A CN102742524A CN 102742524 A CN102742524 A CN 102742524A CN 2012101185932 A CN2012101185932 A CN 2012101185932A CN 201210118593 A CN201210118593 A CN 201210118593A CN 102742524 A CN102742524 A CN 102742524A
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spiral shell
pond
young
close
water
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CN102742524B (en
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章飞军
商弘
付万冬
韩志强
王迎宾
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Zhejiang Ocean University ZJOU
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Zhejiang Ocean University ZJOU
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

Abstract

The invention relates to offspring seed breeding technology of aquaculture, in particular to a fry breeding method for thais luteostoma. Wild adult individuals of the thais luteostoma are collected in an open sea at the temperature of 22-28 DEG C as parent luteostoma, breeding density is 2-5/m<3>, intensive breeding is conducted, eggs are laid, egg capsules are hatched into swimming larva according to hatching density as 3000-6000 egg capsules/m<3>, the swimming larva is fed with swimming larva chrysophyceae, skeletonema costatum, chlorella or chaetoceros according to feeding quantity of 30-100 thousand/mL, after the larva is turned to be baby luteostoma, feeding quantity is10-20 g per time for 1000 young luteostoma, water is changed and disinfected timely, the baby luteostoma is turned to be young luteostoma after 25-45 days, and the young luteostoma can be cultivated. The fry breeding method for the thais luteostoma is simple to operate, achieves scale breeding of thais luteostoma fry, and improves fry yield and survival rate of the thais luteostoma on the basis of reducing cost.

Description

The seedling-cultivating method of a kind of yellow mouthful dog whelk
Technical field
The present invention relates to the seed rearing technology of aquaculture, be specifically related to the seedling-cultivating method of a kind of yellow mouthful dog whelk.
Background technology
Yellow mouthful of dog whelk (Thais luteostoma Holten) belongs to Mollusca (Mollusca), Gastropoda (Gastroroda), narrow tongue order (Stenoglossa), Li Luoke (Thaididae), Purpura (Thais).Yellow mouthful of dog whelk shell median size is spindle, and chitin is solid, and suture is shallow, and is not obvious; Spire is higher, be about shell high 1/2, body whorl expands, the outstanding fillet that forms at the middle part of each gyration; On fillet, have a row angle to strengthen projection, whole shell is looked unfamiliar has many fine and closely woven screw threads and growth line, and the shell face is a lark, the assorted patch that puce is arranged; The long oval of faucal, the inner face khaki, and the patch of a spot of puce is arranged, outer lip is thin; Have that sawtooth is strong to be incised, in sorrowful 3 ~ 4 pieces of dentations are arranged, epipharynx is slightly straight, smooth.Anterior canal is short, tool cutin operculum.Yellow mouthful of dog whelk moves between the cay of intertidal zone low tidal region to the 20 meter depth of water or between gravel.
Yellow mouthful of dog whelk is yellowish pink delicious bright, and protein content is high, and nutritive value is good especially, because coyoting is in recent years extremely caught and the marine pollution aggravation, spiral shell kind resource can not get effective protection, and yellow mouthful dog whelk coastally disappears many.At present, the domestic report that does not also have yellow mouthful of dog whelk artificial breeding makes the large-scale cultivation of yellow mouthful dog whelk can not get progress.How improving the survival rate of the yellow mouthful of dog whelk young, on being emerged in the basis of cost in reduction, improve emergence rate, is the one of the main reasons of yellow mouthful of dog whelk breeding scale of restriction.
Summary of the invention
The seedling-cultivating method that the purpose of this invention is to provide a kind of yellow mouthful of dog whelk, low with the survival rate that solves the present yellow mouthful of dog whelk young, output of seedling is few and the problem of unstable of emerging, thus realize the large-scale cultivation of yellow mouthful dog whelk.
The present invention carries out through following scheme:
The seedling-cultivating method of a kind of yellow mouthful dog whelk may further comprise the steps:
A. prepare in the place of growing seedlings
Parent's spiral shell is cultivated pond, larval culture pond and young spiral shell and is cultivated the pond that the pond is 9 ~ 25 square metres of areas; Parent's spiral shell cultivates the pond and young spiral shell is cultivated dark 1 ~ 1.5 meter of Chi Chi; Dark 1.3 ~ 1.5 meters of larval culture Chi Chi; Parent's spiral shell is cultivated pond, larval culture pond and young spiral shell cultivation pond and is sterilized earlier, and water is gathered seawater by open sea area, and handles after filtration;
In order to improve nursery effect, seawater is carried out filtration treatment, adopt husky well one-level to filter to eliminate impurity, to filter, high-level reservoir deposition and purified treatment are again through the husky filter of secondary.
B. close spiral shell source
In 6 ~ August, be 22 ~ 28 ℃ off-lying sea in ocean temperature, gather yellow mouthful dog whelk and grow up individually as close spiral shell, put into close spiral shell and cultivate the pond and carry out reinforced cultivating;
Wild yellow mouthful of dog whelk kind in the natural sea-water is pure, has better nutritive value than a yellow mouthful dog whelk of propagating artificially.In order to promote the sexual maturity of yellow mouthful dog whelk, so will carry out reinforced cultivating to close spiral shell.
C. the reinforced cultivating of close spiral shell
Regulating close spiral shell cultivation pool water level is 40 ~ 60cm, and water temperature is 24 ℃ ~ 28 ℃, and intensity of illumination is 5000 ~ 15000lx, and oxygen content is 4 ~ 8mg/L, and close spiral shell is put into close spiral shell and cultivated the pond through after sterilizing, and the cultivation density of close spiral shell is 2 ~ 5/m 3, to the close spiral shell shellfish bait of throwing something and feeding, to throw something and feed every day 4 ~ 7 times, each feeding volume is per 10 close spiral shell 20 ~ 30g, and residual bait and impurity is cleaned out in back 3 hours throwing something and feeding, and whenever changes water once at a distance from 2 ~ 3 days, whenever at a distance from 5 ~ 8 days is sterilized once in close spiral shell cultivation pond and close spiral shell;
Reasonably intensity of illumination can improve individual growth rate, and cultivation density is excessive to cause individual competition increase to be unfavorable for that individual growth, the too small meeting of cultivation density cause the waste of culturing the place.Throw something and feed and will in time clear up after the bait, the bait of throwing something and feeding is not owing to can all be eaten up by close spiral shell, and remaining bait can cause water quality to degenerate, and grows germ, endangers the life and health of close spiral shell.
D. the mate and oviposit of close spiral shell
Parent's spiral shell is through 20 ~ 30 days reinforced cultivating after, and gonadal maturation gets into the mate and oviposit stage, and regulating water temperature is 18 ~ 26 ℃, intensity of illumination be reduced to close spiral shell the reinforced cultivating intensity of illumination 60 ~ 70%, after close spiral shell is laid eggs, the collection egg capsule;
The mate and oviposit of parent's spiral shell needs a quiet environment, too high intensity of illumination to be unfavorable for that close spiral shell lays eggs.Before laying eggs, can at the bottom of the pond, lay plastic film, be convenient to collect egg capsule.
E. young hatching and cultivation
Regulating the larval culture pool water level is 90 ~ 120 cm, and water temperature is 26 ~ 30 ℃, and intensity of illumination is 5000 ~ 10000lx, and larval culture pond maritime interior waters is sterilized with 5 ~ 10 ppm potassium permanganate, and ventilation makes dissolved oxygen content be maintained 5 ~ 10mg/L continuously; The egg capsule of collecting is rinsed well with filtering sea earlier, used 20 ~ 40 ppm potassium permanganate soaking disinfections 3 ~ 5 minutes again, put into the larval culture pond then and hatch, hatching density is 3000 ~ 6000 egg capsule/m 3, change water 20 ~ 30% every day, after hatching in 30 ~ 45 days, develop into floating larva; Floating larva is with 2 ~ 30,000/m 3Cultivation density culture; And to floating larva throw something and feed chrysophyceae, middle Skeletonemacostatum, chlorella or Chaetoceros, threw something and fed once in per 8 ~ 12 hours, feeding volume is algae liquid 0.5 ~ 1.5L of every cubic metre of 3 ~ 100,000/mL that throws something and feeds; After 8 ~ 10 days; Feeding volume increases by 30% ~ 50%, after the 15th ~ 22 day, and the young spiral shell of the abnormal one-tenth of floating larva;
Seawater can reduce the disease caused by infectious water bacterial content after sterilizing with potassium permanganate, and egg capsule can be eliminated germ subsidiary on the egg capsule after sterilizing with potassium permanganate, and along with growing up of floating larva, feeding volume increases relatively.
F. young spiral shell is cultivated
The water level of regulating young spiral shell cultivation pond is 50 ~ 80cm, and 26 ~ 30 ℃ of water temperatures, intensity of illumination are 5000 ~ 15000lx, and young spiral shell is cultivated the pond seawater and sterilized with 10 ~ 15 ppm potassium permanganate, and ventilation makes dissolved oxygen content be maintained 5 ~ 12mg/L continuously; Young spiral shell is moved into young spiral shell cultivate the pond, and with 1000 ~ 3000/m 2Density cultivate, throw something and feed the chopping shellfish or shrimps bait, throw something and feed every day 2 ~ 3 times; Feeding volume is per 1000 young spiral shells 10 ~ 20g that at every turn throw something and feed, and every day, 18:00 ~ 20:00 changed water 1 time, and quantity of exchanged water is 30% ~ 50% in preceding 20 days; Quantity of exchanged water is 60% ~ 80% after 20 days; Whenever threw the once sterilizing agent at a distance from 4 ~ 6 days, young spiral shell shell surpasses 5.0 mm, is young spiral shell.
Intensity of illumination, cultivation density play very big influence to the growth rate of young spiral shell; In order to improve the water quality of water, need refresh the water periodically, but a quantity of exchanged water is excessive, it is excessive unfavorable to culturing to cause breeding environment to change; In order to reduce the content of culturing the disease caused by infectious water bacterium, need regularly in water, to throw in disinfectant, thereby reduce the probability of bacteria infection.
As preferably, close spiral shell is cultivated pond, larval culture pond and young spiral shell, and to cultivate the sterilization in pond clean with 12 ~ 24 hours afterflush of liquor potassic permanganate soaking disinfection of 100 ~ 500 ppm.The concentration of potassium permanganate is crossed to hang down and can not effectively be played disinfective action, and excessive concentration can cause potassium permanganate residual more, causes cleaning constant, soaks with liquor potassic permanganate for a long time and can improve sterilisation quality.
As preferably, close spiral shell sterilization adopts the chloromycetin solution of 3 ~ 5ppm to soak 3 ~ 5 minutes among the step c.Excessive concentration can impact close spiral shell, and it is not good to cross low Disinfection Effect, and effectively sterilization can reduce the close spiral shell rate of falling ill.
As preferably, the disinfectant described in the step f is hundred scorching clean, norfloxacins, moroxydine or potassium permanganate, and makes the concentration of throwing in the back disinfectant be respectively 0.3 ~ 0.6ppm, 0.5 ~ 0.8 ppm, 0.4 ~ 0.8 ppm and 5 ~ 10 ppm.The germ in the seawater can be effectively eliminated in the regular input of disinfectant, prevents that the yellow mouthful dog whelk from catching, and improves the survival rate of young spiral shell.
As preferably, the shellfish among step c and the step f is clam, oyster, scallop or Mactra quadrangularis, and shrimps are Penaeus Vannmei, water-white shrimp or pipe whip shrimp.
As preferably, step e and the used seawater of step f also add disodium ethylene diamine tetraacetate, and to make its concentration in seawater be 4 ~ 8ppm.Heavy metal ion can have a negative impact to the growth of yellow mouthful dog whelk; Can improve lethality; Disodium ethylene diamine tetraacetate can reduce the heavy metal concentration in the seawater, and suitable simultaneously concentration can be given full play to the effect that reduces heavy metal concentration under the prerequisite that yellow mouthful dog whelk is not had a negative impact.
Owing to adopt technique scheme, the invention has the beneficial effects as follows: simple to operate and realized the scale breeding of yellow mouthful of dog whelk seedling, on the basis that reduces cost, improved the output of seedling and the survival rate of yellow mouthful of dog whelk seedling.
Embodiment
With specific embodiment the present invention is made further description below.
Embodiment 1
The seedling-cultivating method of a kind of yellow mouthful dog whelk may further comprise the steps:
A. prepare in the place of growing seedlings
Parent's spiral shell is cultivated pond, larval culture pond and young spiral shell and is cultivated the pond that the pond is 9 square metres of areas; Parent's spiral shell cultivates the pond and young spiral shell is cultivated dark 1.2 meters of Chi Chi; Dark 1.5 meters of larval culture Chi Chi; Parent's spiral shell is cultivated pond, larval culture pond and young spiral shell cultivation pond and is sterilized earlier, and sterilization adopts 15 hours afterflush of liquor potassic permanganate soaking disinfection of 100ppm clean.Water is gathered seawater by open sea area, and filters through husky well one-level, and high-level reservoir deposition and purified treatment are again through the husky filter of secondary.
B. close spiral shell source
In June, be 25 ℃ off-lying sea in ocean temperature, gather yellow mouthful dog whelk and grow up individually as close spiral shell, put into close spiral shell and cultivate the pond and carry out reinforced cultivating;
C. the reinforced cultivating of close spiral shell
Regulating close spiral shell cultivation pool water level is 60cm, and water temperature is 24 ℃, and intensity of illumination is 8000lx, and oxygen content is 8mg/L, and close spiral shell is put into close spiral shell and cultivated the pond after adopting the chloromycetin solution of 3ppm to soak sterilization in 4 minutes, and the cultivation density of close spiral shell is 5/m 3,, throw something and feed every day 4 times to the close spiral shell fresh shellfish bait of throwing something and feeding; Each feeding volume is per 10 close spiral shell 25g; And residual bait and impurity are cleaned out in back 3 hours throwing something and feeding, whenever changed water once at a distance from 3 days, whenever at a distance from 5 days close spiral shell is cultivated pond and close spiral shell and adopt with quadrat method and sterilize once;
D. the mate and oviposit of close spiral shell
Parent's spiral shell is through 25 days reinforced cultivating after, and gonadal maturation gets into the mate and oviposit stage, and regulating water temperature is 26 ℃, intensity of illumination be reduced to close spiral shell the reinforced cultivating intensity of illumination 60%, after laying eggs, the collection egg capsule;
E. young hatching and cultivation
Regulating the larval culture pool water level is 100 cm; Water temperature is 30 ℃, and intensity of illumination is 5000lx, and larval culture pond maritime interior waters is sterilized with 8ppm potassium permanganate; In addition with the disodium ethylene diamine tetraacetate of 8ppm to eliminate heavy metal ion, keeping ventilation to make dissolved oxygen content is 5mg/L; The egg capsule of collecting is rinsed well with filtering sea earlier, used 30 ppm potassium permanganate soaking disinfections 5 minutes again, place in the 60 purposes hatching net then and put into the larval culture pond and hatch, hatching density is 5000 egg capsule/m 3, change water 30% every day, after hatching in 30 ~ 45 days, fertilized egg develops into floating larva in succession; Floating larva is with 20,000/m 3Cultivation density culture, and, threw something and fed once in per 8 hours to floating larva throw something and feed chrysophyceae, middle Skeletonemacostatum, chlorella or Chaetoceros; Feeding volume is the algae liquid 0.8L of every cubic metre of 3 ~ 100,000/mL that throws something and feeds, and feeding volume increases by 30%, after the 18th day; The young spiral shell of the abnormal one-tenth of floating larva
F. young spiral shell is cultivated
The water level of regulating young spiral shell cultivation pond is 80 cm; 26 ℃ of water temperatures, intensity of illumination are 7000lx, and young spiral shell is cultivated the pond seawater and sterilized with 15ppm potassium permanganate; In addition with the disodium ethylene diamine tetraacetate of 4ppm to eliminate heavy metal ion, keeping ventilation continuously to make dissolved oxygen content is 8mg/L; Young spiral shell is moved into young spiral shell cultivate the pond, and with 2500/m 2Density cultivate, the shellfish or the shrimps bait of the chopping of throwing something and feeding, shellfish is clam, oyster, scallop or Mactra quadrangularis, shrimps are Penaeus Vannmei, water-white shrimp or pipe whip shrimp; Throw something and feed every day 3 times, feeding volume is per 1000 young spiral shells 10g that at every turn throw something and feed, and every day, 18:00 ~ 20:00 changed water 1 time; Quantity of exchanged water is 50% in 18 days early stages, and the later stage quantity of exchanged water is 60%, whenever throws one time hundred inflammation only at a distance from 5 days; Making its concentration is 0.3ppm, and young spiral shell shell surpasses 5.0 mm, is young spiral shell.
Embodiment 2
The seedling-cultivating method of a kind of yellow mouthful dog whelk may further comprise the steps:
A. prepare in the place of growing seedlings
Parent's spiral shell is cultivated pond, larval culture pond and young spiral shell and is cultivated the pond that the pond is 20 square metres of areas; Parent's spiral shell cultivates the pond and young spiral shell is cultivated dark 1.5 meters of Chi Chi; Dark 1.3 meters of larval culture Chi Chi; Parent's spiral shell is cultivated pond, larval culture pond and young spiral shell cultivation pond and is sterilized earlier, and sterilization adopts 24 hours afterflush of liquor potassic permanganate soaking disinfection of 300 ppm clean.Water is gathered seawater by open sea area, and handles through filtering; Sea water specific gravity is 1.020, and pH is 8.0.
B. close spiral shell source
In 6 ~ August, be 28 ℃ off-lying sea in ocean temperature, gather yellow mouthful dog whelk and grow up individually as close spiral shell, put into close spiral shell and cultivate the pond and carry out reinforced cultivating;
C. the reinforced cultivating of close spiral shell
Regulating close spiral shell cultivation pool water level is 40cm, and water temperature is 26 ℃, and intensity of illumination is 15000lx, and oxygen content is 4mg/L, and close spiral shell is put into close spiral shell and cultivated the pond after adopting the chloromycetin solution of 4ppm to soak sterilization in 5 minutes, and the cultivation density of close spiral shell is 2/m 3,, throw something and feed every day 6 times to the close spiral shell fresh shellfish bait of throwing something and feeding; Each feeding volume is per 10 close spiral shell 30g; And residual bait and foreign material are cleaned out in back 3 hours throwing something and feeding, whenever changed water once at a distance from 2 days, whenever at a distance from 6 days close spiral shell is cultivated pond and close spiral shell and adopt with quadrat method and sterilize once;
D. the mate and oviposit of close spiral shell
Parent's spiral shell is through 30 days reinforced cultivating after, and gonadal maturation gets into the mate and oviposit stage, and regulating water temperature is 18 ℃, intensity of illumination be reduced to close spiral shell the reinforced cultivating intensity of illumination 70%, after laying eggs, the collection egg capsule;
E. young hatching and cultivation
Regulating the larval culture pool water level is 120 cm; Water temperature is 26 ℃, and intensity of illumination is 7000lx, and larval culture pond maritime interior waters is sterilized with 10 ppm potassium permanganate; In addition with the disodium ethylene diamine tetraacetate of 4ppm to eliminate heavy metal ion, keeping ventilation to make dissolved oxygen content is 8mg/L; The egg capsule of collecting is rinsed well with filtering sea earlier, used 40 ppm potassium permanganate soaking disinfections 3 minutes again, place in the 75 purposes hatching net then and put into the larval culture pond and hatch, hatching density is 6000 egg capsule/m 3, change water 20% every day, after hatching in 30 ~ 45 days, fertilized egg develops into floating larva in succession; Floating larva is with 30,000/m 3Cultivation density culture, and, threw something and fed once in per 10 hours to floating larva throw something and feed chrysophyceae, middle Skeletonemacostatum, chlorella or Chaetoceros; Feeding volume is the algae liquid 1L of every cubic metre of 3 ~ 100,000/mL that throws something and feeds, and after throwing something and feeding 8 days, feeding volume increases by 40%; After the 22nd day, the young spiral shell of the abnormal one-tenth of floating larva
F. young spiral shell is cultivated
The water level of regulating young spiral shell cultivation pond is 50cm; 28 ℃ of water temperatures, intensity of illumination are 15000lx, and young spiral shell is cultivated the pond seawater and sterilized with 10ppm potassium permanganate; In addition with the disodium ethylene diamine tetraacetate of 6ppm to eliminate heavy metal ion, keeping ventilation continuously to make dissolved oxygen content is 12mg/L; Young spiral shell is moved into young spiral shell cultivate the pond, and with 2000/m 2Density cultivate, the shellfish or the shrimps bait of the chopping of throwing something and feeding, shellfish is clam, oyster, scallop or Mactra quadrangularis, shrimps are Penaeus Vannmei, water-white shrimp or pipe whip shrimp; Throw something and feed every day 2 times, feeding volume is per 1000 young spiral shells 15g that at every turn throw something and feed, and every day, 18:00 ~ 20:00 changed water 1 time; Quantity of exchanged water was 30% in 20 days early stages, and the later stage quantity of exchanged water is 70%, whenever threw a norfloxacin at a distance from 6 days; Making its concentration is 0.5ppm, and young spiral shell shell surpasses 5.0 mm, is young spiral shell.
Embodiment 3
The seedling-cultivating method of a kind of yellow mouthful dog whelk may further comprise the steps:
A. prepare in the place of growing seedlings
Parent's spiral shell is cultivated pond, larval culture pond and young spiral shell and is cultivated the pond that the pond is 25 square metres of areas; Parent's spiral shell cultivates the pond and young spiral shell is cultivated dark 1 meter of Chi Chi; Dark 1.4 meters of larval culture Chi Chi; Parent's spiral shell is cultivated pond, larval culture pond and young spiral shell cultivation pond and is sterilized earlier, and sterilization adopts 12 hours afterflush of liquor potassic permanganate soaking disinfection of 500 ppm clean.Water is gathered seawater by open sea area, and handles through filtering; Sea water specific gravity is 1.020 ~ 1.023, and pH is 8.5.
B. close spiral shell source
In 6 ~ August, be 22 ℃ off-lying sea in ocean temperature, gather yellow mouthful dog whelk and grow up individually as close spiral shell, put into close spiral shell and cultivate the pond and carry out reinforced cultivating;
C. the reinforced cultivating of close spiral shell
Regulating close spiral shell cultivation pool water level is 50 cm, and water temperature is 28 ℃, and intensity of illumination is 5000lx, and oxygen content is 6mg/L, and close spiral shell is put into close spiral shell and cultivated the pond after adopting the chloromycetin solution of 5ppm to soak sterilization in 3 minutes, and the cultivation density of close spiral shell is 4/m 3,, throw something and feed every day 7 times to the close spiral shell fresh shellfish bait of throwing something and feeding; Each feeding volume is per 10 close spiral shell 20g; And residual bait and foreign material are cleaned out in back 3 hours throwing something and feeding, whenever changed water once at a distance from 3 days, whenever at a distance from 8 days close spiral shell is cultivated pond and close spiral shell and adopt with quadrat method and sterilize once;
D. the mate and oviposit of close spiral shell
Parent's spiral shell is through 20 days reinforced cultivating after, and gonadal maturation gets into the mate and oviposit stage, and regulating water temperature is 22 ℃, intensity of illumination be reduced to close spiral shell the reinforced cultivating intensity of illumination 65%, after laying eggs, the collection egg capsule;
E. young hatching and cultivation
Regulating the larval culture pool water level is 90cm; Water temperature is 28 ℃, and intensity of illumination is 10000lx, and larval culture pond maritime interior waters is sterilized with 5ppm potassium permanganate; In addition with the disodium ethylene diamine tetraacetate of 6ppm to eliminate heavy metal ion, keeping ventilation to make dissolved oxygen content is 10mg/L; The egg capsule of collecting is rinsed well with filtering sea earlier, used 20ppm potassium permanganate soaking disinfection 4 minutes again, place in the 100 purposes hatching net then and put into the larval culture pond and hatch, hatching density is 3000 egg capsule/m 3, change water 25% every day, after hatching in 30 ~ 45 days, fertilized egg develops into floating larva in succession; Floating larva is with 2.5 ten thousand/m 3Cultivation density culture, and, threw something and fed once in per 12 hours to floating larva throw something and feed chrysophyceae, middle Skeletonemacostatum, chlorella or Chaetoceros; Feeding volume is the algae liquid 0.5L of every cubic metre of 3 ~ 100,000/mL that throws something and feeds; After throwing something and feeding 9 days, feeding volume increases by 50%, after the 15th day; The young spiral shell of the abnormal one-tenth of floating larva
F. young spiral shell is cultivated
The water level of regulating young spiral shell cultivation pond is 60 cm; 30 ℃ of water temperatures, intensity of illumination are 5000lx, and young spiral shell is cultivated the pond seawater and sterilized with 12ppm potassium permanganate; In addition with the disodium ethylene diamine tetraacetate of 8ppm to eliminate heavy metal ion, keeping ventilation continuously to make dissolved oxygen content is 5mg/L; Young spiral shell is moved into young spiral shell cultivate the pond, and with 3000/m 2Density cultivate, the shellfish or the shrimps bait of the chopping of throwing something and feeding, shellfish is clam, oyster, scallop or Mactra quadrangularis, shrimps are Penaeus Vannmei, water-white shrimp or pipe whip shrimp; Throw something and feed every day 2 times, feeding volume is per 1000 young spiral shells 20g that at every turn throw something and feed, and every day, 18:00 ~ 20:00 changed water 1 time; Quantity of exchanged water was 40% in 15 days early stages, and the later stage quantity of exchanged water is 80%, whenever threw a moroxydine at a distance from 4 days; Making its concentration is 0.8 ppm, and young spiral shell shell surpasses 5.0 mm, is young spiral shell.
Embodiment 4
The seedling-cultivating method of a kind of yellow mouthful dog whelk may further comprise the steps:
A. prepare in the place of growing seedlings
Parent's spiral shell is cultivated pond, larval culture pond and young spiral shell and is cultivated the pond that the pond is 25 square metres of areas; Parent's spiral shell cultivates the pond and young spiral shell is cultivated dark 1 meter of Chi Chi; Dark 1.4 meters of larval culture Chi Chi; Parent's spiral shell is cultivated pond, larval culture pond and young spiral shell cultivation pond and is sterilized earlier, and sterilization adopts 12 hours afterflush of liquor potassic permanganate soaking disinfection of 500 ppm clean.Water is gathered seawater by open sea area, and handles through filtering; Sea water specific gravity is 1.020 ~ 1.023, and pH is 8.5.
B. close spiral shell source
In 6 ~ August, be 22 ℃ off-lying sea in ocean temperature, gather yellow mouthful dog whelk and grow up individually as close spiral shell, put into close spiral shell and cultivate the pond and cultivate;
C. the reinforced cultivating of close spiral shell
Regulating close spiral shell cultivation pool water level is 50 cm, and water temperature is 28 ℃, and intensity of illumination is 5000lx, and oxygen content is 6mg/L, and close spiral shell is put into close spiral shell and cultivated the pond after adopting the chloromycetin solution of 5ppm to soak sterilization in 3 minutes, and the cultivation density of close spiral shell is 4/m 3,, throw something and feed every day 7 times to the close spiral shell fresh shellfish bait of throwing something and feeding; Each feeding volume is per 10 close spiral shell 20g; And residual bait and foreign material are cleaned out in back 3 hours throwing something and feeding, whenever changed water once at a distance from 3 days, whenever at a distance from 8 days close spiral shell is cultivated pond and close spiral shell and adopt with quadrat method and sterilize once;
D. the mate and oviposit of close spiral shell
Parent's spiral shell is through 20 days reinforced cultivating after, and gonadal maturation gets into the mate and oviposit stage, and regulating water temperature is 22 ℃, intensity of illumination be reduced to close spiral shell the reinforced cultivating intensity of illumination 62%, after laying eggs, the collection egg capsule;
E. young hatching and cultivation
Regulating the larval culture pool water level is 90cm; Water temperature is 28 ℃, and intensity of illumination is 10000lx, and larval culture pond maritime interior waters is sterilized with 5ppm potassium permanganate; In addition with the disodium ethylene diamine tetraacetate of 6ppm to eliminate heavy metal ion, keeping ventilation to make dissolved oxygen content is 10mg/L; The egg capsule of collecting is rinsed well with filtering sea earlier, used 20ppm potassium permanganate soaking disinfection 4 minutes again, place in the 100 purposes hatching net then and put into the larval culture pond and hatch, hatching density is 3000 egg capsule/m 3, change water 25% every day, after hatching in 30 ~ 45 days, fertilized egg develops into floating larva in succession; Floating larva is with 2.3 ten thousand/m 3Cultivation density culture, and, threw something and fed once in per 12 hours to floating larva throw something and feed chrysophyceae, middle Skeletonemacostatum, chlorella or Chaetoceros; Feeding volume is the algae liquid 1.5L of every cubic metre of 3 ~ 100,000/mL that throws something and feeds; After throwing something and feeding 9 days, feeding volume increases by 50%, after the 15th day; The young spiral shell of the abnormal one-tenth of floating larva
F. young spiral shell is cultivated
The water level of regulating young spiral shell cultivation pond is 60cm; 30 ℃ of water temperatures, intensity of illumination are 5000lx, and young spiral shell is cultivated the pond seawater and sterilized with 12ppm potassium permanganate; In addition with the disodium ethylene diamine tetraacetate of 8ppm to eliminate heavy metal ion, keeping ventilation continuously to make dissolved oxygen content is 5mg/L; Young spiral shell is moved into young spiral shell cultivate the pond, and with 1000/m 2Density cultivate, throw something and feed the chopping shellfish or shrimps bait, shellfish is clam, oyster, scallop or Mactra quadrangularis; Shrimps are Penaeus Vannmei, water-white shrimp or pipe whip shrimp, throw something and feed every day 2 times, and feeding volume is per 1000 young spiral shells 20g that at every turn throw something and feed; Every day, 18:00 ~ 20:00 changed water 1 time, and quantity of exchanged water is 40% in 15 days early stages, and the later stage quantity of exchanged water is 80%; Whenever threw a potassium permanganate disinfectant at a distance from 4 days, making its concentration is 8ppm, and young spiral shell shell surpasses 5.0 mm; Be young spiral shell, can be used as seedling for breed.
Have to draw a conclusion after adopting seedling-cultivating method of the present invention to grow seedlings: among 4 embodiment; The incubation rate of ovum is respectively 85.6%, 87.2%, 80.9% and 84.1%; Survival rate after the ovum hatching is that emergence rate is respectively 88.3%, 91.5%, 90.8% and 93.1%, and the young spiral shell of every square meter is cultivated the pond can emerge 1000 ~ 2500.

Claims (6)

1. the seedling-cultivating method of a yellow mouthful dog whelk is characterized in that, may further comprise the steps:
A. prepare in the place of growing seedlings
Parent's spiral shell is cultivated pond, larval culture pond and young spiral shell and is cultivated the pond that the pond is 9 ~ 25 square metres of areas; Parent's spiral shell cultivates the pond and young spiral shell is cultivated dark 1 ~ 1.5 meter of Chi Chi; Dark 1.3 ~ 1.5 meters of larval culture Chi Chi; Parent's spiral shell is cultivated pond, larval culture pond and young spiral shell cultivation pond and is sterilized earlier, and water is gathered seawater by open sea area, and handles after filtration;
B. close spiral shell source
In 6 ~ August, be 22 ~ 28 ℃ off-lying sea in ocean temperature, gather yellow mouthful dog whelk and grow up individually as close spiral shell, put into close spiral shell and cultivate the pond and carry out reinforced cultivating;
C. the reinforced cultivating of close spiral shell
Regulating close spiral shell cultivation pool water level is 40 ~ 60 cm, and water temperature is 24 ℃ ~ 28 ℃, and intensity of illumination is 5000 ~ 15000lx, and oxygen content is 4 ~ 8mg/L, and close spiral shell is put into close spiral shell and cultivated the pond through after sterilizing, and the cultivation density of close spiral shell is 2 ~ 5/m 3, to the close spiral shell shellfish bait of throwing something and feeding, to throw something and feed every day 4 ~ 7 times, each feeding volume is per 10 close spiral shell 20 ~ 30g, and residual bait and impurity is cleaned out in back 3 hours throwing something and feeding, and whenever changes water once at a distance from 2 ~ 3 days, whenever at a distance from 5 ~ 8 days is sterilized once in close spiral shell cultivation pond and close spiral shell;
D. the mate and oviposit of close spiral shell
Parent's spiral shell is through 20 ~ 30 days reinforced cultivating after, and gonadal maturation gets into the mate and oviposit stage, and regulating water temperature is 18 ~ 26 ℃, intensity of illumination be reduced to close spiral shell the reinforced cultivating intensity of illumination 60 ~ 70%, after close spiral shell is laid eggs, the collection egg capsule;
E. young hatching and cultivation
Regulating the larval culture pool water level is 90 ~ 120 cm, and water temperature is 26 ~ 30 ℃, and intensity of illumination is 5000 ~ 10000lx, and larval culture pond maritime interior waters is sterilized with 5 ~ 10 ppm potassium permanganate, and ventilation makes dissolved oxygen content be maintained 5 ~ 10mg/L continuously; The egg capsule of collecting is rinsed well with filtering sea earlier, used 20 ~ 40 ppm potassium permanganate soaking disinfections 3 ~ 5 minutes again, put into the larval culture pond then and hatch, hatching density is 3000 ~ 6000 egg capsule/m 3, change water 20 ~ 30% every day, after hatching in 30 ~ 45 days, develop into floating larva; Floating larva is with 2 ~ 30,000/m 3Cultivation density culture, and, threw something and fed once in per 8 ~ 12 hours to floating larva throw something and feed chrysophyceae, middle Skeletonemacostatum, chlorella or Chaetoceros; Feeding volume is algae liquid 0.5 ~ 1.5L of every cubic metre of 3 ~ 100,000/mL that throws something and feeds; After 8 ~ 10 days, feeding volume increases by 30% ~ 50%, after the 15th ~ 22 day; The young spiral shell of the abnormal one-tenth of floating larva
F. young spiral shell is cultivated
The water level of regulating young spiral shell cultivation pond is 50 ~ 80 cm, and 26 ~ 30 ℃ of water temperatures, intensity of illumination are 5000 ~ 15000lx, and young spiral shell is cultivated the pond seawater and sterilized with 10 ~ 15 ppm potassium permanganate, and ventilation makes dissolved oxygen content be maintained 5 ~ 12mg/L continuously; Young spiral shell is moved into young spiral shell cultivate the pond, and with 1000 ~ 3000/m 2Density cultivate, throw something and feed the chopping shellfish or shrimps bait, throw something and feed every day 2 ~ 3 times; Feeding volume is per 1000 young spiral shells 10 ~ 20g that at every turn throw something and feed, and every day, 18:00 ~ 20:00 changed water 1 time, and quantity of exchanged water is 30% ~ 50% in preceding 20 days; Quantity of exchanged water is 60% ~ 80% after 20 days; Whenever threw the once sterilizing agent at a distance from 4 ~ 6 days, young spiral shell shell surpasses 5.0 mm, is young spiral shell.
2. the seedling-cultivating method of a kind of yellow mouthful dog whelk according to claim 1 is characterized in that, it is clean with 12 ~ 24 hours afterflush of liquor potassic permanganate soaking disinfection of 100 ~ 500 ppm that close spiral shell is cultivated the sterilization in pond, larval culture pond and young spiral shell cultivation pond.
3. the seedling-cultivating method of a kind of yellow mouthful dog whelk according to claim 1 and 2 is characterized in that, close spiral shell sterilization adopts the chloromycetin solution of 3 ~ 5 ppm to soak 3 ~ 5 minutes among the step c.
4. the seedling-cultivating method of a kind of yellow mouthful dog whelk according to claim 1 and 2; It is characterized in that; Disinfectant described in the step f is hundred scorching clean, norfloxacin, moroxydine or potassium permanganate, and makes the concentration of throwing in the back disinfectant be respectively 0.3 ~ 0.6ppm, 0.5 ~ 0.8 ppm, 0.4 ~ 0.8 ppm and 5 ~ 10 ppm.
5. the seedling-cultivating method of a kind of yellow mouthful dog whelk according to claim 1 and 2 is characterized in that the shellfish among step c and the step f is clam, oyster, scallop or Mactra quadrangularis, and shrimps are Penaeus Vannmei, water-white shrimp or pipe whip shrimp.
6. the seedling-cultivating method of a kind of yellow mouthful of dog whelk according to claim 1 and 2 is characterized in that step e and the used seawater of step f also add disodium ethylene diamine tetraacetate, and to make its concentration in seawater be 4 ~ 8ppm.
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CN110810287A (en) * 2019-12-24 2020-02-21 福建省壹方海生态农业开发有限公司 Artificial breeding method of peppery snail
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CN111771782A (en) * 2020-07-03 2020-10-16 中国水产科学研究院东海水产研究所 Artificial seedling culture method for yellow mud snails

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