CN115362891A - Tissue culture breeding method for new high-yield Henwang wheat variety - Google Patents
Tissue culture breeding method for new high-yield Henwang wheat variety Download PDFInfo
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- CN115362891A CN115362891A CN202210989416.5A CN202210989416A CN115362891A CN 115362891 A CN115362891 A CN 115362891A CN 202210989416 A CN202210989416 A CN 202210989416A CN 115362891 A CN115362891 A CN 115362891A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/20—Cereals
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H3/00—Processes for modifying phenotypes, e.g. symbiosis with bacteria
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Abstract
The invention discloses a tissue culture breeding method of a new high-yield King wheat variety, which comprises the following steps: s1: selecting seeds, namely selecting full wheat seeds; s2: vernalization, namely placing wheat seeds in a low-temperature bud box; s3: moving the wheat seedlings into an intelligent climate control room; s4: planting, namely planting the seedlings in nutrient soil; s5: and (3) performing later-stage management, wherein the seed selection in the step S1 is to put seeds into a water container filled with water, the temperature of a low-temperature bud box in the step S2 is controlled to be 0-5 ℃ to perform forced vernalization on the wheat seeds, the seedling accelerating time of wheat in the step S2 is controlled to be within 35-45 days, and the wheat seedlings are about 2-6 centimeters higher. By breeding through a breeding method, a new ultra-high yield wheat strain with stable offspring is obtained, the drought resistance is better, the light efficiency is high, and the yield of the wheat is high; the cultivation method is changed to shorten the cultivation time, effectively improve the speed of cultivating new varieties and effectively improve the tissue culture breeding efficiency of the new variety of the Juwang wheat.
Description
Technical Field
The invention relates to the technical field of wheat, in particular to a tissue culture breeding method of a new high-yield King wheat variety.
Background
Wheat is a general name of wheat plants, a representative species of wheat is common wheat, which is a gramineae plant and is a cereal crop widely planted around the world, wheat caryopsis is one of staple food of human beings, the wheat caryopsis is ground into flour to be made into bread, steamed bread, biscuits, noodles and other foods, the flour can be made into beer, alcohol, white spirit or biomass fuel after fermentation, the wheat is one of three grains, almost all grains are eaten, and only about one sixth of the grains is used as feed.
The existing tissue culture breeding method of a new high-yield King wheat variety causes the problem of low wheat yield caused indirectly by poor drought resistance and high light efficiency of wheat.
Disclosure of Invention
Technical problem to be solved
Aiming at the defects of the prior art, the invention provides a tissue culture breeding method of a new high-yield King wheat variety, which has the advantages of promoting the yield of wheat to be high and the like and solves the problems in the background technology.
(II) technical scheme
In order to realize the aim of tissue culture breeding of the new high-yield King wheat variety, the invention provides the following technical scheme: a tissue culture breeding method of a new high-yield Juanwang wheat variety comprises the following steps:
s1: selecting seeds, namely selecting full wheat seeds;
s2: vernalization, namely placing wheat seeds in a low-temperature bud box;
s3: moving the wheat seedlings into an intelligent climate control room;
s4: planting, planting the seedlings in nutrient soil;
s5: and (5) performing post-management.
Preferably, the seed selection in the S1 is performed by putting seeds into a water container filled with the water container, observing whether the wheat seeds can be deposited at the bottom of the water container in the container or not, wherein the wheat seeds are half-winterness, medium-early maturing, the maturation period of the wheat seeds is 1-2 days earlier than that of Yumai No. 49 and New mai No. 18, the seedlings are upright, the leaves are wide and green, the tillering force is medium, the seedlings are early to rise and pull out in spring, the seedlings grow rapidly, the two poles differentiate rapidly, the heading is early, the heading rate is general, the plant height is about 77 cm, the plant type is compact, the flag leaves are wide and curled, the spike layers are neat, the long phase is clear, the plant is long and square, the awn, the white husk, the white grains, the kernel semi-cutin, the plumpness is general, the black embryo rate is medium, the average ear number per mu is 36.6, the ear number is 37.6, the thousand weight is 39.5 g, the seedling stage is strong, the spring strength is medium, and the seeds are sensitive to cold in the drought season. The stem has good elasticity, good lodging resistance, high temperature resistance in the later period, early maturity and good maturity, and is a monocotyledon.
Preferably, the temperature of the low-temperature malt box in the S2 is controlled at 0-5 ℃ to perform forced vernalization on the wheat seeds, the vernalization refers to a phenomenon that the plants need to undergo continuous low temperature for a period of time to be transferred from a vegetative growth stage to a reproductive stage for growth, and the vernalization of the wheat seeds is beneficial to later cultivation of new wheat varieties.
Preferably, the wheat seedling accelerating time in the S2 is controlled within 35-45 days, and the wheat seedling is about 2-6 centimeters higher.
Preferably, the temperature in the intelligent climate control chamber in the step S3 is kept between 25 and 35 degrees, and the humidity is between 55 and 95%, and the intelligent artificial climate chamber can simulate various meteorological conditions in nature, can simulate and monitor the temperature, the humidity and the illuminance in real time, and meets the requirements of artificial tests.
Preferably, the nutrient soil in the S4 is covered to 5-8mm above of the seedlings, and the nutrient incubator is subjected to light-tight treatment.
Preferably, the temperature of the nutrient incubator in S4 is controlled to 20 to 30 degrees, and the incubation is performed for 35 to 40 days.
Preferably, water is sprayed every 1.5-3.5 hours in the S5 until the wheat grows out of the first leaf, and the water spraying is stopped.
(III) advantageous effects
Compared with the prior art, the invention provides a tissue culture breeding method of a new high-yield King wheat variety, which has the following beneficial effects:
1. the tissue culture breeding method of the new high-yield King wheat variety obtains the new ultrahigh-yield wheat variety with stable progeny through breeding by a breeding method, has better drought resistance and high light efficiency, and has high wheat yield.
2. According to the tissue culture breeding method of the high-yield Hewang wheat new variety, the breeding time is shortened by changing the breeding method, the speed of breeding the new variety is effectively increased, and the tissue culture breeding efficiency of the Hewang wheat new variety is effectively increased.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The first embodiment is as follows:
s1: selecting relatively full wheat seeds, wherein the seed selection in S1 is carried out by putting the seeds into a water container filled with the water container, observing whether the wheat seeds can be deposited at the bottom of the water container or not, wherein the wheat seeds are half-winter, medium-early maturing and 1-2 days earlier in maturation period than Yumai No. 49 and New mai No. 18, seedlings are upright, leaves are wide and dark green, tillering force is medium, spring rising and node pulling are early, growth is rapid, two poles are fast to differentiate, heading is early, heading rate is general, plant height is about 77 cm, plant type is compact, flag leaves are wide and curled, spike layers are tidy, long-phase is clear and beautiful, spikes are square, curly awns, white shells, white grains, kernel half cutin, plumpness is general, black embryo rate is medium, average number of mu is 36.6 ten thousand spikes, spike number is 37.6, thousand weight is 39.5 g, long grain period is strong, spring seedling strength is medium, and is sensitive to low temperature in spring seasons. The stem has good elasticity, good lodging resistance, high temperature resistance in the later period, early maturity and good maturity, is a monocotyledon, so that good seeds are very necessary to be selected.
S2: vernalization, namely placing wheat seeds in a low-temperature bud box, controlling the temperature of the low-temperature bud box in S2 to be 0-5 ℃ to perform forced vernalization on the wheat seeds, wherein vernalization refers to the phenomenon that plants need to go through continuous low temperature for a period of time to be transferred from a vegetative growth stage to a reproductive stage for growth, the vernalization of the wheat seeds is beneficial to later cultivation of new wheat varieties, the seedling accelerating time of the wheat in S2 is controlled within 35-45 days, and the wheat seedlings are about 2-6 centimeters higher;
s3: the wheat seedlings are moved into an intelligent climate control chamber, the temperature in the intelligent climate control chamber in S3 is kept between 25 and 35 ℃, the humidity is between 55 and 95 percent, the intelligent artificial climate chamber can simulate various meteorological conditions in nature, the temperature, the humidity and the illuminance can be simulated and monitored in real time, and the requirements of artificial tests are met;
s4: planting, namely planting the seedlings in nutrient soil, covering the nutrient soil in the S4 to a depth of more than 5-8mm of the seedlings, and closing the light of a nutrient incubator in the S4, controlling the temperature of the nutrient incubator in the S4 to be 20-30 ℃, and culturing for 35-40 days;
s5: and (3) performing later-stage management, namely spraying water every 1.5-3.5 hours in S5 until the first leaf of the wheat grows out, stopping spraying water, and breeding by using the breeding method, so that a new ultrahigh-yield wheat strain with stable progeny is obtained, the new ultrahigh-yield wheat strain has better drought resistance and high light efficiency, the yield of the wheat is high, the breeding time is shortened by changing the breeding method, the speed of breeding a new variety is effectively improved, and the tissue culture breeding efficiency of the new King wheat variety is effectively improved.
The second embodiment:
s1: selecting relatively full wheat seeds, wherein the seed selection in S1 is carried out by putting the seeds into a water container filled with the water container, observing whether the wheat seeds can be deposited at the bottom of the water container or not, wherein the wheat seeds are half-winter, medium-early maturing and 1-2 days earlier in maturation period than Yumai No. 49 and New mai No. 18, seedlings are upright, leaves are wide and dark green, tillering force is medium, spring rising and node pulling are early, growth is rapid, two poles are fast to differentiate, heading is early, heading rate is general, plant height is about 77 cm, plant type is compact, flag leaves are wide and curled, spike layers are tidy, long-phase is clear and beautiful, spikes are square, curly awns, white shells, white grains, kernel half cutin, plumpness is general, black embryo rate is medium, average number of mu is 36.6 ten thousand spikes, spike number is 37.6, thousand weight is 39.5 g, long grain period is strong, spring seedling strength is medium, and is sensitive to low temperature in spring seasons. The stem has good elasticity, good lodging resistance, high temperature resistance in the later period, early maturity and good maturity, is a monocotyledon, so that good seeds are selected.
S2: vernalization, namely placing wheat seeds in a low-temperature bud box, wherein the temperature of the low-temperature bud box in S2 is controlled to be 0-5 ℃ to perform forced vernalization on the wheat seeds, the vernalization refers to the phenomenon that a plant can be transferred from a vegetative growth stage to a reproductive stage to grow only after a period of continuous low temperature, the vernalization of the wheat seeds is beneficial to later cultivation of new wheat varieties, the seedling accelerating time of the wheat in S2 is 37 days, and the wheat seedlings are about 3 centimeters higher;
s3: the wheat seedlings are moved into the intelligent climate control chamber, the temperature in the intelligent climate control chamber in S3 is kept at 27 ℃, the humidity is 65%, the intelligent artificial climate chamber can simulate various meteorological conditions in nature, the temperature, the humidity and the illuminance can be simulated and monitored in real time, and the requirements of artificial tests are met;
s4: planting, namely planting the seedlings in nutrient soil, covering the nutrient soil in the S4 to a position of more than 5-8mm of the seedlings, and closing the light of a nutrient incubator, controlling the temperature of the nutrient incubator in the S4 to be 22 ℃, and culturing for 36 days;
s5: and (3) performing later-stage management, namely spraying water every 1.5-3.5 hours in S5 until the first leaf of the wheat grows out, stopping spraying water, and breeding by using the breeding method, so that a new ultrahigh-yield wheat strain with stable progeny is obtained, the new ultrahigh-yield wheat strain has better drought resistance and high light efficiency, the yield of the wheat is high, the breeding time is shortened by changing the breeding method, the speed of breeding a new variety is effectively improved, and the tissue culture breeding efficiency of the new King wheat variety is effectively improved.
Example three:
s1: selecting relatively full wheat seeds, wherein the seed selection in S1 is to place the seeds into a water container filled with the water container, and select the wheat seeds by observing whether the wheat seeds can be deposited at the bottom of the water container, the wheat seeds are semi-winter, medium-early maturing and have the maturation period 1-2 days earlier than that of contrast Yumai No. 49 and New wheat, seedlings are upright, leaves are wide and blue, the leaf color is dark green, the tillering force is medium, the rising and the node pulling are early in spring, the growth is rapid, the two-pole differentiation is rapid, the heading rate is early, the heading rate is general, the plant height is about 77 cm, the plant type is compact, the flag leaves are wide and curled, the spike layers are regular, the long-phase is clear and beautiful, the spikes are long and square, the awn, the white shells, the white grains, the kernel semi-cutin, the plumpness is general, the black embryo rate is medium, the average number of spikes is 36.6, the number of spikes is 37.6, the thousand kernel weight is 39.5 g, the seedling stage is strong, the cold resistance is medium, and the wheat seeds are sensitive to low temperature in spring seasons. The stem has good elasticity, good lodging resistance, high temperature resistance in the later period, early maturity and good maturity, is a monocotyledon, so that good seeds are selected.
S2: vernalization, namely placing wheat seeds in a low-temperature bud box, wherein the temperature of the low-temperature bud box in S2 is controlled to be 0-5 ℃ to perform forced vernalization on the wheat seeds, the vernalization refers to the phenomenon that a plant can be transferred from a vegetative growth stage to a reproductive stage to grow only after a period of continuous low temperature, the vernalization of the wheat seeds is beneficial to later cultivation of new wheat varieties, the seedling accelerating time of the wheat in S2 is controlled to be within 39 days, and the wheat seedlings are about 4 centimeters higher;
s3: the wheat seedlings are moved into the intelligent climate control chamber, the temperature in the intelligent climate control chamber in S3 is kept at 29 ℃, the humidity is 75%, the intelligent artificial climate chamber can simulate various meteorological conditions in the nature, the temperature, the humidity and the illuminance can be simulated and monitored in real time, and the requirements of artificial tests are met;
s4: planting, namely planting the seedlings in nutrient soil, covering the nutrient soil in the S4 to the position of more than 5-8mm of the seedlings, and closing the light of a nutrient incubator, controlling the temperature of the nutrient incubator in the S4 to be 24 ℃, and culturing for 37 days;
s5: and (3) performing later-stage management, namely spraying water every 1.5-3.5 hours in S5 until the first leaf of the wheat grows out, stopping spraying water, and breeding by using the breeding method, so that a new ultrahigh-yield wheat strain with stable progeny is obtained, the new ultrahigh-yield wheat strain has better drought resistance and high light efficiency, the yield of the wheat is high, the breeding time is shortened by changing the breeding method, the speed of breeding a new variety is effectively improved, and the tissue culture breeding efficiency of the new King wheat variety is effectively improved.
Experimental example four:
s1: selecting relatively full wheat seeds, wherein the seed selection in S1 is to place the seeds into a water container filled with the water container, and select the wheat seeds by observing whether the wheat seeds can be deposited at the bottom of the water container, the wheat seeds are semi-winter, medium-early maturing and have the maturation period 1-2 days earlier than that of contrast Yumai No. 49 and New wheat, seedlings are upright, leaves are wide and blue, the leaf color is dark green, the tillering force is medium, the rising and the node pulling are early in spring, the growth is rapid, the two-pole differentiation is rapid, the heading rate is early, the heading rate is general, the plant height is about 77 cm, the plant type is compact, the flag leaves are wide and curled, the spike layers are regular, the long-phase is clear and beautiful, the spikes are long and square, the awn, the white shells, the white grains, the kernel semi-cutin, the plumpness is general, the black embryo rate is medium, the average number of spikes is 36.6, the number of spikes is 37.6, the thousand kernel weight is 39.5 g, the seedling stage is strong, the cold resistance is medium, and the wheat seeds are sensitive to low temperature in spring seasons. The stem has good elasticity, good lodging resistance, high temperature resistance in the later period, early maturity and good maturity, is a monocotyledon, so that good seeds are selected.
S2: vernalization, namely placing wheat seeds in a low-temperature bud box, wherein the temperature of the low-temperature bud box in S2 is controlled to be 0-5 ℃ to perform forced vernalization on the wheat seeds, the vernalization refers to the phenomenon that a plant can be transferred from a vegetative growth stage to a reproductive stage to grow only after a period of continuous low temperature, the vernalization of the wheat seeds is beneficial to later cultivation of new wheat varieties, the seedling accelerating time of the wheat in S2 is controlled to be 41 days, and the wheat seedlings are about 5 centimeters higher;
s3: the wheat seedlings are moved into the intelligent climate control chamber, the temperature in the intelligent climate control chamber in S3 is kept at 31 ℃, the humidity is 85%, the intelligent artificial climate chamber can simulate various meteorological conditions in nature, the temperature, the humidity and the illuminance can be simulated and monitored in real time, and the requirements of artificial tests are met;
s4: planting, namely planting the seedlings in nutrient soil, covering the nutrient soil in the S4 to a position above 5-8mm of the seedlings, and closing the light of a nutrient incubator, controlling the temperature of the nutrient incubator in the S4 to be 26 ℃, and culturing for 38 days;
s5: and (3) performing later-stage management, namely spraying water every 1.5-3.5 hours in S5 until the first leaf of the wheat grows out, stopping spraying water, and breeding by using the breeding method, so that a new ultrahigh-yield wheat strain with stable progeny is obtained, the new ultrahigh-yield wheat strain has better drought resistance and high light efficiency, the yield of the wheat is high, the breeding time is shortened by changing the breeding method, the speed of breeding a new variety is effectively improved, and the tissue culture breeding efficiency of the new King wheat variety is effectively improved.
Experimental example five:
s1: selecting relatively full wheat seeds, wherein the seed selection in S1 is to place the seeds into a water container filled with the water container, and select the wheat seeds by observing whether the wheat seeds can be deposited at the bottom of the water container, the wheat seeds are semi-winter, medium-early maturing and have the maturation period 1-2 days earlier than that of contrast Yumai No. 49 and New wheat, seedlings are upright, leaves are wide and blue, the leaf color is dark green, the tillering force is medium, the rising and the node pulling are early in spring, the growth is rapid, the two-pole differentiation is rapid, the heading rate is early, the heading rate is general, the plant height is about 77 cm, the plant type is compact, the flag leaves are wide and curled, the spike layers are regular, the long-phase is clear and beautiful, the spikes are long and square, the awn, the white shells, the white grains, the kernel semi-cutin, the plumpness is general, the black embryo rate is medium, the average number of spikes is 36.6, the number of spikes is 37.6, the thousand kernel weight is 39.5 g, the seedling stage is strong, the cold resistance is medium, and the wheat seeds are sensitive to low temperature in spring seasons. The stem has good elasticity, good lodging resistance, high temperature resistance in the later period, early maturity and good maturity, is a monocotyledon, so that good seeds are selected.
S2: vernalization, namely placing wheat seeds in a low-temperature bud box, wherein the temperature of the low-temperature bud box in S2 is controlled to be 0-5 ℃ to perform forced vernalization on the wheat seeds, the vernalization refers to the phenomenon that a plant can be transferred from a vegetative growth stage to a reproductive stage to grow only after a period of continuous low temperature, the vernalization of the wheat seeds is beneficial to later cultivation of new wheat varieties, the seedling accelerating time of the wheat in S2 is controlled to be within 43 days, and the wheat seedlings are about 6 centimeters higher;
s3: the wheat seedlings are moved into the intelligent climate control chamber, the temperature in the intelligent climate control chamber in S3 is kept at 33 ℃, the humidity is 95%, the intelligent artificial climate chamber can simulate various meteorological conditions in the nature, the temperature, the humidity and the illuminance can be simulated and monitored in real time, and the requirements of artificial tests are met;
s4: planting, namely planting the seedlings in nutrient soil, covering the nutrient soil in the S4 to the position of more than 5-8mm of the seedlings, and closing the light of a nutrient incubator, wherein the temperature of the nutrient incubator in the S4 is controlled to be 28 ℃, and the seedlings are cultured for 40 days;
s5: and (3) performing later-stage management, namely spraying water every 1.5-3.5 hours in S5 until the first leaf of the wheat grows out, stopping spraying water, and breeding by using the breeding method, so that a new ultrahigh-yield wheat strain with stable progeny is obtained, the new ultrahigh-yield wheat strain has better drought resistance and high light efficiency, the yield of the wheat is high, the breeding time is shortened by changing the breeding method, the speed of breeding a new variety is effectively improved, and the tissue culture breeding efficiency of the new King wheat variety is effectively improved.
The invention has the beneficial effects that: the breeding method is adopted for breeding, so that the new ultrahigh-yield wheat strain with stable progeny is obtained, the drought resistance is better, the light efficiency is high, the wheat yield is high, the breeding time is shortened by changing the breeding method, the speed of breeding new varieties is effectively improved, and the efficiency of tissue culture breeding of the new variety of Juwang wheat is effectively improved.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (8)
1. A tissue culture breeding method of a new high-yield Juanwang wheat variety is characterized by comprising the following steps:
s1: selecting seeds, namely selecting full wheat seeds;
s2: vernalization, namely placing wheat seeds in a low-temperature bud box;
s3: moving the wheat seedlings into an intelligent climate control room;
s4: planting, namely planting the seedlings in nutrient soil;
s5: and (5) performing post-management.
2. The tissue culture breeding method of a new high yield King wheat variety as claimed in claim 1, wherein the selection in S1 is performed by putting seeds into a water container filled with water, and observing whether the wheat seeds can be settled to the bottom of the water container.
3. The tissue culture breeding method of a new high yield King wheat variety according to claim 1, wherein the temperature of the low temperature shoot box in S2 is controlled at a low temperature of 0-5 ℃ to perform forced vernalization on wheat seeds.
4. The tissue culture breeding method of a new high-yield King wheat variety according to claim 1, wherein the seedling accelerating time of the wheat in S2 is controlled within 35-45 days, and the height of the wheat seedling is about 2-6 cm.
5. The tissue culture breeding method of the new high yield King wheat variety according to claim 1, wherein the temperature in the intelligent climate control chamber in S3 is kept between 25 and 35 degrees, and the humidity is between 55 and 95%.
6. The tissue culture breeding method of a new high-yield King wheat variety as claimed in claim 1, wherein the nutrient soil in S4 is covered to 5-8mm above the seedling, and the nutrient incubator is subjected to light-tight treatment.
7. The tissue culture breeding method of a new high-yield King wheat variety according to claim 1, wherein the temperature of the nutrient incubator in S4 is controlled to be 20-30 ℃ and the cultivation lasts for 35-40 days.
8. The tissue culture breeding method of a new high yield King wheat variety according to claim 1, wherein water is sprayed every 1.5-3.5 hours in S5 until the first leaf of the wheat grows out.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN116171941A (en) * | 2023-03-08 | 2023-05-30 | 福建省农业科学院水稻研究所 | Application of wheat seedlings in cold storage of trichogramma longsynanthassemblies and cold storage method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN116171941A (en) * | 2023-03-08 | 2023-05-30 | 福建省农业科学院水稻研究所 | Application of wheat seedlings in cold storage of trichogramma longsynanthassemblies and cold storage method |
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Application publication date: 20221122 |