CN106804270A - A kind of breeding method of high yield, disease-resistant, anti-fall new variety of wheat - Google Patents
A kind of breeding method of high yield, disease-resistant, anti-fall new variety of wheat Download PDFInfo
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- CN106804270A CN106804270A CN201710037444.6A CN201710037444A CN106804270A CN 106804270 A CN106804270 A CN 106804270A CN 201710037444 A CN201710037444 A CN 201710037444A CN 106804270 A CN106804270 A CN 106804270A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C21/00—Methods of fertilising, sowing or planting
- A01C21/005—Following a specific plan, e.g. pattern
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H3/00—Processes for modifying phenotypes, e.g. symbiosis with bacteria
- A01H3/04—Processes for modifying phenotypes, e.g. symbiosis with bacteria by treatment with chemicals
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
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- Soil Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Organic Chemistry (AREA)
- Botany (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a kind of high yield, the breeding method of disease-resistant, anti-fall new variety of wheat, step is as follows：（1）Wheat to break be put into 0~3 degree of low temperature bud case after dormancy vernalization carries out pressure vernalization, high 3 centimeters；（2）The food potato cut off with one end is cultivated；（3）Intelligent biotron children plants, and wheat is cut off from tillering node with sterilization blade, retains wheat tillering section top and is allocated as scion；（4）Grafting hole is got out in the port of potato cut-out, grafting hole and surrounding are sterilized；（5）By in scion insertion grafting hole；（6）：In the nutrition incubator of immigration sterilization treatment；（7）Artificial intelligence climatic chamber is moved into, wheat seed is harvested；（8）By wheat seed as first filial generation seed, after plantation is selected, second filial seed is obtained；（9）After the plantation of second filial seed, F3 seed is obtained.The present invention improves the yield of new varieties wheat, improves the Optimality such as disease-resistant, anti-fall of wheat, and then increased income, and satisfaction is actually needed.
Description
Technical field
The present invention relates to a kind of high yield, the breeding method of disease-resistant, anti-fall new variety of wheat, belong to new variety of wheat cultivation side
Law technology field.
Background technology
Wheat is one of three big cereal, is a kind of staple food crop widely planted in the world, yield almost full conduct
It is edible.Wheat is the general designation of wheat system plant, is monocotyledon, is a kind of grass family plant in plantation extensively all over the world
Thing, the caryopsis of wheat is one of staple food of the mankind, and the foods such as bread, steamed bun, biscuit, noodles can be made after wearing into flour；Fermentation
After can be made into beer, alcohol, white wine（Such as vodka）, or raw matter fuel.Wheat rich in starch, protein, fat, mineral matter,
Calcium, iron, thiamine, riboflavin, nicotinic acid, vitamin A and vitamin C etc..Wheat is one of three big cereal, is almost eaten entirely,
1/6th are only there are about to be used as feed.The Yellow Basin and the Yangtze River is the area of earliest cultivated wheat in the world, and China is the earliest world
Plant one of country of wheat.Wheat in 2010 is the cereal crops (6.51 hundred million tons) that total output occupies second in the world, only secondary
In corn (8.44 hundred million tons).
At present：New variety of wheat is without the good characteristic such as high yield, disease-resistant, anti-fall, additionally, often due to prior art is closed
In breeding method it is incorrect, manage it is incorrect and cause cultivate new varieties wheat seed quality dissatisfaction, so may be just
Bring unnecessary economic loss.For this reason, it may be necessary to improve a kind of new breeding method, above-mentioned existing skill comprehensive can be overcome
The deficiency that art is present.
The content of the invention
The present invention is exactly in view of the shortcomings of the prior art, there is provided a kind of high yield, the training of disease-resistant, anti-fall new variety of wheat
Method is educated, this method changes the breeding method of conventional wheat, improves the yield of new varieties wheat, improves new varieties wheat
The Optimality such as disease-resistant, anti-fall, and then income is increased, satisfaction is actually needed.
To solve the above problems, the technical solution used in the present invention is as follows：
A kind of breeding method of high yield, disease-resistant, anti-fall new variety of wheat, the breeding method step is as follows：
Step（1）：Wheat to break be put into 0~3 degree of low temperature bud case after dormancy vernalization carries out pressure vernalization, completes small after 35~40 days
The pressure vernalization of wheat, now wheat is high 3 centimeters in seedling stage；
Step（2）：Plantation food potato, when food potato grows to 6~10cm, by the cut-out of potato one end（Cut-out
Distance is 0.8~1.2cm）；
Step（3）：The potato that wheat seedling, one end cut off is moved in calm aseptic intelligent biotron, intelligent weather
Between control temperature to be maintained at 20~30 degree in control room, humidity be 60~80%, with blade of sterilizing by wheat from tillering node
Cut-out, retains wheat tillering section top and is allocated as scion, the cut-off part sterilization to wheat；
Step（4）：The potato cut off with one end is cooked stock, is bored with the drill bit of 1.5mm in the port position of potato cut-out
Go out grafting hole, grafting hole depth is the 2/5~3/5 of potato thickness, and grafting hole and surrounding position are sterilized；
Step（5）：By in scion insertion grafting hole, will together be tightly wrapped as the potato of stock and scion with medical proof fabric；
Step（6）：Move into the nutrition incubator of sterilization treatment, by the potato planting of scion in Nutrition Soil, Nutrition Soil covers
To more than grafting hole 6~12mm, nutrition incubator black out, temperature is 20~25 degree to lid, is cultivated 26~32 days；
When having just enter into nutrition incubator, the potato to scion carries out atomizing spray, the water of spray in every 1~3 hour, until wheat
When growing first piece leaf, stop water spray；
The nutrient solution being made into nutrient, auxin and the basic element of cell division is applied fertilizer, every sprinkling in 5 days once, spray concentration
It is 200ppm；
Step（7）：Artificial intelligence climatic chamber is moved into, wheat late growing stage phase condition is simulated, indoor temperature is 26~32 degree, humidity
Be 60~80%, daytime natural light, evening, wheat was ripe after 80 days, can harvest Wheat Species with simulated light 7~11 hours
Son；
Step（8）：By wheat seed as first filial generation seed, 50% is used for field planting, therefrom obtains the plant of variation, 50%
Used as seed, the potato cut off with one end again carries out grafting backcrossing, obtains second filial seed；
Step（9）：It is used for field planting by the 50% of second filial seed, therefrom obtains the plant of variation, 50% used as seed, the
Grafting backcrossing is carried out with one end cut-out potato three times, obtain F3 seed.
Used as the improvement of above-mentioned technical proposal, in step 3 and step 4, what food potato, scion were carried out disinfection is molten
Liquid is absolute alcohol, and its concentration is 94%.
Used as the improvement of above-mentioned technical proposal, in step 6, described nutrient solution is nutrient, auxin and cell point
Split element to be uniformly mixed, its mass ratio is 1:1:1.
Compared with prior art, implementation result of the invention is as follows for the present invention：
The breeding method of a kind of high yield of the present invention, disease-resistant, anti-fall new variety of wheat, be according to principle, biological epigenetic
Think：Any one cell with certain biological whole hereditary information has the potential for developing into intact organism, also
To say, each biological cell have totipotency the characteristics of（Contain into starch 9~20%% in general potato, protein 1.5~
2.3%%, fat 0.1~1.1%%, crude fibre 0.6~0.8%%.Contained nutritional ingredient in 100g potatos：Heat 66~
113J, 11~60mg of calcium, 15~68mg of phosphorus, iron 0.4mg~4.8mg, 0.03~0.07mg of thiamine, riboflavin 0.03~
0.11mg, 0.4~1.1mg of niacin, with abundant trophic function；Additionally, the trace element contained in potato has resisting
Sick, anti-fall effect）.Therefore, in theory, any one biological cell all has the potentiality for developing into whole plant.This hair
Bright high yield, the breeding method of disease-resistant, anti-fall new variety of wheat are analog resistant mutant, and asexual hybridization does not change in breeding process
Gene order, but gene expression there occurs heritable change, and this change can be steady during development and cell proliferation
Fixed transmission, so as to obtain high yield, disease-resistant, anti-fall Progenies of Wheat new lines.General breeding method needs the 8-10 could to cultivate
The new varieties for going out, and breeding method of the invention shorten to triennium, can just cultivate a new varieties, so as to shorten
Breeding time, improves and cultivates new varieties speed.
Specific embodiment
Illustrate present disclosure below in conjunction with specific embodiments.
Embodiment 1
A kind of breeding method of high yield, disease-resistant, anti-fall new variety of wheat, the breeding method step is as follows：
Step（1）：Wheat to break be put into 0 degree of low temperature bud case after dormancy vernalization carries out pressure vernalization, and the strong of wheat is completed after 35 days
Vernalization processed, now wheat is high 3 centimeters in seedling stage；
Step（2）：Plantation food potato, when food potato grows to 6cm, by the cut-out of potato one end（Breaking distance
It is 0.8cm）；
Step（3）：The potato that wheat seedling, one end cut off is moved in calm aseptic intelligent biotron, intelligent weather
Between control temperature to be maintained at 20 degree in control room, humidity be 60%, wheat is cut off from tillering node with sterilization blade, reservation
Wheat tillering section top is allocated as scion, the cut-off part sterilization to wheat；
Step（4）：The potato cut off with one end is cooked stock, is bored with the drill bit of 1.5mm in the port position of potato cut-out
Go out grafting hole, grafting hole depth is the 2/5 of potato thickness, and grafting hole and surrounding position are sterilized；
Step（5）：By in scion insertion grafting hole, will together be tightly wrapped as the potato of stock and scion with medical proof fabric；
Step（6）：Move into the nutrition incubator of sterilization treatment, by the potato planting of scion in Nutrition Soil, Nutrition Soil covers
To grafting hole above 6mm, nutrition incubator black out, temperature is 20 degree to lid, is cultivated 26 days；
When having just enter into nutrition incubator, the potato to scion carries out atomizing spray, the water of spray in every 1 hour, until wheat is long
When going out first piece leaf, stop water spray；
The nutrient solution being made into nutrient, auxin and the basic element of cell division is applied fertilizer, every sprinkling in 5 days once, spray concentration
It is 200ppm；
Step（7）：Artificial intelligence climatic chamber is moved into, wheat late growing stage phase condition is simulated, indoor temperature is 26 degree, humidity is
60%, daytime natural light, evening, wheat was ripe after 80 days, can harvest wheat seed with simulated light 7 hours；
Step（8）：By wheat seed as first filial generation seed, 50% is used for field planting, therefrom obtains the plant of variation, 50%
Used as seed, the potato cut off with one end again carries out grafting backcrossing, obtains second filial seed；
Step（9）：It is used for field planting by the 50% of second filial seed, therefrom obtains the plant of variation, 50% used as seed, the
Grafting backcrossing is carried out with one end cut-out potato three times, obtain F3 seed.
More specifically, in step 3 and step 4, the solution carried out disinfection to food potato, scion is absolute alcohol,
Its concentration is 94%；In step 6, described nutrient solution is uniformly mixed for nutrient, auxin and the basic element of cell division,
Its mass ratio is 1:1:1.
The present invention is that biological epigenetics thinks according to principle：With certain biological whole hereditary information any one
Individual cell has the potential for developing into intact organism, that is to say, that the characteristics of each biological cell has totipotency（Typically
Contain into starch 9~20%%, protein 1.5~2.3%%, fat 0.1~1.1%%, crude fibre 0.6~0.8%% in potato.100g
Contained nutritional ingredient in potato：66~113J of heat, 11~60mg of calcium, 15~68mg of phosphorus, iron 0.4mg~4.8mg, thiamines
Element 0.03~0.07mg, 0.03~0.11mg of riboflavin, 0.4~1.1mg of niacin, with abundant trophic function；Additionally, horse
The trace element contained in bell potato has disease-resistant, anti-fall effect）.Therefore, in theory, any one biological cell all has
Develop into the potentiality of whole plant.High yield of the invention, the breeding method of disease-resistant, anti-fall new variety of wheat are analog resistant mutant, nothing
Property hybridization, do not change gene order in breeding process, but gene expression there occurs heritable change, and this change exists
Energy stable delivery during development and cell proliferation, so as to obtain high yield, disease-resistant, anti-fall Progenies of Wheat new lines.General training
The new varieties that method needs 8-10 just to cultivate are educated, and breeding method of the invention shorten to triennium, with regard to energy
A new varieties are cultivated, so as to shorten breeding time, is improved and is cultivated new varieties speed.
Embodiment 2
A kind of breeding method of high yield, disease-resistant, anti-fall new variety of wheat, the breeding method step is as follows：
Step（1）：Wheat to break be put into 1~2 degree of low temperature bud case after dormancy vernalization carries out pressure vernalization, completes small after 37~38 days
The pressure vernalization of wheat, now wheat is high 3 centimeters in seedling stage；
Step（2）：Plantation food potato, when food potato grows to 8cm, by the cut-out of potato one end（Breaking distance
It is 1cm）；
Step（3）：The potato that wheat seedling, one end cut off is moved in calm aseptic intelligent biotron, intelligent weather
Between control temperature to be maintained at 25 degree in control room, humidity be 70%, wheat is cut off from tillering node with sterilization blade, reservation
Wheat tillering section top is allocated as scion, the cut-off part sterilization to wheat；
Step（4）：The potato cut off with one end is cooked stock, is bored with the drill bit of 1.5mm in the port position of potato cut-out
Go out grafting hole, grafting hole depth is the 1/2 of potato thickness, and grafting hole and surrounding position are sterilized；
Step（5）：By in scion insertion grafting hole, will together be tightly wrapped as the potato of stock and scion with medical proof fabric；
Step（6）：Move into the nutrition incubator of sterilization treatment, by the potato planting of scion in Nutrition Soil, Nutrition Soil covers
To grafting hole above 9mm, nutrition incubator black out, temperature is 22~23 degree to lid, is cultivated 29 days；
When having just enter into nutrition incubator, the potato to scion carries out atomizing spray, the water of spray in every 2 hours, until wheat is long
When going out first piece leaf, stop water spray；
The nutrient solution being made into nutrient, auxin and the basic element of cell division is applied fertilizer, every sprinkling in 5 days once, spray concentration
It is 200ppm；
Step（7）：Artificial intelligence climatic chamber is moved into, wheat late growing stage phase condition is simulated, indoor temperature is 29 degree, humidity is
70%, daytime natural light, evening, wheat was ripe after 80 days, can harvest wheat seed with simulated light 9 hours；
Step（8）：By wheat seed as first filial generation seed, 50% is used for field planting, therefrom obtains the plant of variation, 50%
Used as seed, the potato cut off with one end again carries out grafting backcrossing, obtains second filial seed；
Step（9）：It is used for field planting by the 50% of second filial seed, therefrom obtains the plant of variation, 50% used as seed, the
Grafting backcrossing is carried out with one end cut-out potato three times, obtain F3 seed.
More specifically, in step 3 and step 4, the solution carried out disinfection to food potato, scion is absolute alcohol,
Its concentration is 94%；In step 6, described nutrient solution is uniformly mixed for nutrient, auxin and the basic element of cell division,
Its mass ratio is 1:1:1.
The present invention is that biological epigenetics thinks according to principle：With certain biological whole hereditary information any one
Individual cell has the potential for developing into intact organism, that is to say, that the characteristics of each biological cell has totipotency（Typically
Contain into starch 9~20%%, protein 1.5~2.3%%, fat 0.1~1.1%%, crude fibre 0.6~0.8%% in potato.100g
Contained nutritional ingredient in potato：66~113J of heat, 11~60mg of calcium, 15~68mg of phosphorus, iron 0.4mg~4.8mg, thiamines
Element 0.03~0.07mg, 0.03~0.11mg of riboflavin, 0.4~1.1mg of niacin, with abundant trophic function；Additionally, horse
The trace element contained in bell potato has disease-resistant, anti-fall effect）.Therefore, in theory, any one biological cell all has
Develop into the potentiality of whole plant.High yield of the invention, the breeding method of disease-resistant, anti-fall new variety of wheat are analog resistant mutant, nothing
Property hybridization, do not change gene order in breeding process, but gene expression there occurs heritable change, and this change exists
Energy stable delivery during development and cell proliferation, so as to obtain high yield, disease-resistant, anti-fall Progenies of Wheat new lines.General training
The new varieties that method needs 8-10 just to cultivate are educated, and breeding method of the invention shorten to triennium, with regard to energy
A new varieties are cultivated, so as to shorten breeding time, is improved and is cultivated new varieties speed.
Embodiment 3
A kind of breeding method of high yield, disease-resistant, anti-fall new variety of wheat, the breeding method step is as follows：
Step（1）：Wheat to break be put into 3 degree of low temperature bud case after dormancy vernalization carries out pressure vernalization, and the strong of wheat is completed after 40 days
Vernalization processed, now wheat is high 3 centimeters in seedling stage；
Step（2）：Plantation food potato, when food potato grows to 10cm, by the cut-out of potato one end（Breaking distance
It is 1.2cm）；
Step（3）：The potato that wheat seedling, one end cut off is moved in calm aseptic intelligent biotron, intelligent weather
Between control temperature to be maintained at 30 degree in control room, humidity be 80%, wheat is cut off from tillering node with sterilization blade, reservation
Wheat tillering section top is allocated as scion, the cut-off part sterilization to wheat；
Step（4）：The potato cut off with one end is cooked stock, is bored with the drill bit of 1.5mm in the port position of potato cut-out
Go out grafting hole, grafting hole depth is the 3/5 of potato thickness, and grafting hole and surrounding position are sterilized；
Step（5）：By in scion insertion grafting hole, will together be tightly wrapped as the potato of stock and scion with medical proof fabric；
Step（6）：Move into the nutrition incubator of sterilization treatment, by the potato planting of scion in Nutrition Soil, Nutrition Soil covers
To grafting hole above 12mm, nutrition incubator black out, temperature is 25 degree to lid, is cultivated 32 days；
When having just enter into nutrition incubator, the potato to scion carries out atomizing spray, the water of spray in every 3 hours, until wheat is long
When going out first piece leaf, stop water spray；
The nutrient solution being made into nutrient, auxin and the basic element of cell division is applied fertilizer, every sprinkling in 5 days once, spray concentration
It is 200ppm；
Step（7）：Artificial intelligence climatic chamber is moved into, wheat late growing stage phase condition is simulated, indoor temperature is 32 degree, humidity is
80%, daytime natural light, evening, wheat was ripe after 80 days, can harvest wheat seed with simulated light 11 hours；
Step（8）：By wheat seed as first filial generation seed, 50% is used for field planting, therefrom obtains the plant of variation, 50%
Used as seed, the potato cut off with one end again carries out grafting backcrossing, obtains second filial seed；
Step（9）：It is used for field planting by the 50% of second filial seed, therefrom obtains the plant of variation, 50% used as seed, the
Grafting backcrossing is carried out with one end cut-out potato three times, obtain F3 seed.
More specifically, in step 3 and step 4, the solution carried out disinfection to food potato, scion is absolute alcohol,
Its concentration is 94%；In step 6, described nutrient solution is uniformly mixed for nutrient, auxin and the basic element of cell division,
Its mass ratio is 1:1:1.
The present invention is that biological epigenetics thinks according to principle：With certain biological whole hereditary information any one
Individual cell has the potential for developing into intact organism, that is to say, that the characteristics of each biological cell has totipotency（Typically
Contain into starch 9~20%%, protein 1.5~2.3%%, fat 0.1~1.1%%, crude fibre 0.6~0.8%% in potato.100g
Contained nutritional ingredient in potato：66~113J of heat, 11~60mg of calcium, 15~68mg of phosphorus, iron 0.4mg~4.8mg, thiamines
Element 0.03~0.07mg, 0.03~0.11mg of riboflavin, 0.4~1.1mg of niacin, with abundant trophic function；Additionally, horse
The trace element contained in bell potato has disease-resistant, anti-fall effect）.Therefore, in theory, any one biological cell all has
Develop into the potentiality of whole plant.High yield of the invention, the breeding method of disease-resistant, anti-fall new variety of wheat are analog resistant mutant, nothing
Property hybridization, do not change gene order in breeding process, but gene expression there occurs heritable change, and this change exists
Energy stable delivery during development and cell proliferation, so as to obtain high yield, disease-resistant, anti-fall Progenies of Wheat new lines.General training
The new varieties that method needs 8-10 just to cultivate are educated, and breeding method of the invention shorten to triennium, with regard to energy
A new varieties are cultivated, so as to shorten breeding time, is improved and is cultivated new varieties speed.
Note：Design parameter span can be according to actual conditions in each step in cultural method of the present invention（Can be according to tool
The yield of the wheat reached needed for body, disease-resistant, anti-fall, drought-enduring, waterlogging tolerance energy etc.）Setting.
Above content is detailed description made for the present invention in conjunction with specific embodiments, it is impossible to assert that the present invention is specific real
Apply and be only limitted to these explanations.For those skilled in the art, before present inventive concept is not departed from
Put, some simple deduction or replace can also be made, should all be considered as belonging to the scope of protection of the invention.
Claims (3)
1. a kind of high yield, the breeding method of disease-resistant, anti-fall new variety of wheat, it is characterised in that：The breeding method step is as follows：
Step（1）：Wheat to break be put into 0~3 degree of low temperature bud case after dormancy vernalization carries out pressure vernalization, completes small after 35~40 days
The pressure vernalization of wheat, now wheat is high 3 centimeters in seedling stage；
Step（2）：Plantation food potato, when food potato grows to 6~10cm, by the cut-out of potato one end（Cut-out
Distance is 0.8~1.2cm）；
Step（3）：The potato that wheat seedling, one end cut off is moved in calm aseptic intelligent biotron, intelligent weather
Between control temperature to be maintained at 20~30 degree in control room, humidity be 60~80%, with blade of sterilizing by wheat from tillering node
Cut-out, retains wheat tillering section top and is allocated as scion, the cut-off part sterilization to wheat；
Step（4）：The potato cut off with one end is cooked stock, is bored with the drill bit of 1.5mm in the port position of potato cut-out
Go out grafting hole, grafting hole depth is the 2/5~3/5 of potato thickness, and grafting hole and surrounding position are sterilized；
Step（5）：By in scion insertion grafting hole, will together be tightly wrapped as the potato of stock and scion with medical proof fabric；
Step（6）：Move into the nutrition incubator of sterilization treatment, by the potato planting of scion in Nutrition Soil, Nutrition Soil covers
To more than grafting hole 6~12mm, nutrition incubator black out, temperature is 20~25 degree to lid, is cultivated 26~32 days；
When having just enter into nutrition incubator, the potato to scion carries out atomizing spray, the water of spray in every 1~3 hour, until wheat
When growing first piece leaf, stop water spray；
The nutrient solution being made into nutrient, auxin and the basic element of cell division is applied fertilizer, every sprinkling in 5 days once, spray concentration
It is 200ppm；
Step（7）：Artificial intelligence climatic chamber is moved into, wheat late growing stage phase condition is simulated, indoor temperature is 26~32 degree, humidity
Be 60~80%, daytime natural light, evening, wheat was ripe after 80 days, can harvest Wheat Species with simulated light 7~11 hours
Son；
Step（8）：By wheat seed as first filial generation seed, 50% is used for field planting, therefrom obtains the plant of variation, 50%
Used as seed, the potato cut off with one end again carries out grafting backcrossing, obtains second filial seed；
Step（9）：It is used for field planting by the 50% of second filial seed, therefrom obtains the plant of variation, 50% used as seed, the
Grafting backcrossing is carried out with one end cut-out potato three times, obtain F3 seed.
2. a kind of high yield, the breeding method of disease-resistant, anti-fall new variety of wheat according to claim 1, it is characterised in that：In step
Rapid 3 and step 4 in, the solution carried out disinfection to food potato, scion be absolute alcohol, its concentration be 94%.
3. a kind of high yield, the breeding method of disease-resistant, anti-fall new variety of wheat according to claim 1, it is characterised in that：In step
In rapid 6, described nutrient solution is uniformly mixed for nutrient, auxin and the basic element of cell division, and its mass ratio is 1:1:1.
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| CN201710037444.6A CN106804270A (en) | 2017-01-19 | 2017-01-19 | A kind of breeding method of high yield, disease-resistant, anti-fall new variety of wheat |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112931089A (en) * | 2021-01-28 | 2021-06-11 | 河南多麦稼农业科技有限公司 | Technology for cultivating lodging-resistant wheat by utilizing adversity stress distant symbiosis and epigenetic inheritance |
| CN112931092A (en) * | 2021-01-28 | 2021-06-11 | 河南多麦稼农业科技有限公司 | Technology for cultivating hexaploid purple-kernel wheat by utilizing distant symbiosis and epigenetic inheritance |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104170644A (en) * | 2014-08-31 | 2014-12-03 | 曹洪青 | Method for cultivating new species of super-high-yield wheat |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104170644A (en) * | 2014-08-31 | 2014-12-03 | 曹洪青 | Method for cultivating new species of super-high-yield wheat |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112931089A (en) * | 2021-01-28 | 2021-06-11 | 河南多麦稼农业科技有限公司 | Technology for cultivating lodging-resistant wheat by utilizing adversity stress distant symbiosis and epigenetic inheritance |
| CN112931092A (en) * | 2021-01-28 | 2021-06-11 | 河南多麦稼农业科技有限公司 | Technology for cultivating hexaploid purple-kernel wheat by utilizing distant symbiosis and epigenetic inheritance |
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