CN110679478B - Breeding method of forage grass type BMR sorghum sterile line - Google Patents
Breeding method of forage grass type BMR sorghum sterile line Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
Abstract
A breeding method of forage grass type BMR sorghum sterile line belongs to the technical field of forage grass variety breeding methods. Hybridizing BMR genotype sorghum maintainer lines as female parent and forage grass type sorghum maintainer lines with tillering property as male parent, and breeding forage grass type BMR sorghum maintainer lines firstly, wherein the standard is as follows: brown midrib; the plant height is 140-160 cm; the total number of the leaves is not less than 30; the tillering performance is high, and the effective tillering number of a single plant is 2-4; the height difference between the tillering stem and the main stem is not more than 5cm, and the flowering phase difference is not more than 7 days; the property of the spike is good, and the spike length is 25-30 cm; tightening the spike shape; the setting rate is more than 98%. And then carrying out backcross transformation with a common sorghum sterile line, and selecting a completely sterile BMR single plant consistent with the agronomic characters of a forage type BMR sorghum maintainer line from a backcross group to finally obtain the forage type BMR sorghum sterile line. The forage type BMR sorghum sterile line bred by the invention enriches the gene bank of the sorghum sterile line.
Description
Technical Field
The invention belongs to the technical field of pasture variety breeding methods, relates to a breeding method of forage type sorghum, and particularly relates to a breeding method of a forage type BMR sorghum sterile line.
Background
Sorghum sudanense (Sorghum bicolor x Sorghum sudanense) is a distant hybrid with a Sorghum (Sorghum bicolor (L.) Moench) sterile line as a female parent and sudan grass (Sorghum sudanense (pepper) Stapf) as a male parent, but the common Sorghum sterile line is expressed as: short stalk, less total leaf number, no tillering property, poor regeneration after cutting, and no accordance with forage grass utilization; since sorghum sudanense is used as a forage grass, the aim is to harvest nutrients, and ground biomass such as stems and leaves is pursued, some sorghum sterile lines with a large number of leaves, strong tillering property and reproducibility need to be created to improve the feeding quality of sorghum sudanense.
The Gaodan grass species can be divided into two types according to the color of the leaf veins, and the color of the midrib of the leaf veins is white, which is called white vein; the color of the veins in the leaves is light green, which is called wax veins. The lignification degree of the stems of the two varieties is high in the late stage of grouting, and the digestibility is reduced, so that the feeding quality of the two varieties is gradually reduced. The content of lignin which is difficult to digest in sorghum bodies and has the characteristics of Brown Midrib (BMR) can be reduced by 40-60%, the digestion rate can be improved by about 30%, and the feeding quality of the sorghum can be greatly improved. Based on the actual situation that the new germplasm of the forage BMR sorghum sterile line is lacked at present, and the corresponding breeding method, technology and standard are not reported, the production requirement is difficult to meet, so that the creation of the sorghum sterile line with the BMR genotype (brown midrib) has important significance for breeding the BMR genotype Gaodan grass variety suitable for feeding.
Disclosure of Invention
The invention provides a breeding method of a forage grass type BMR sorghum sterile line in order to overcome the defects of the prior art, which carries out breeding innovation by taking the forage grass characteristics and the BMR characteristics into consideration, so as to innovate the forage grass type BMR sorghum sterile line, has important significance for breeding a new variety of BMR genotype Gaodan grass and improving the quality of forage, and has wide application prospect.
The technical scheme adopted by the invention is as follows: a breeding method of forage grass type BMR sorghum sterile line comprises the following steps of carrying out biparental hybridization, pedigree breeding and continuous backcross to obtain the forage grass type BMR sorghum sterile line:
(1) breeding of forage grass type BMR sorghum maintainer line
Taking a sorghum maintainer line of BMR genotype as a female parent and a forage grass type sorghum maintainer line with tillerability as a male parent, obtaining F0 generation hybrid seeds through castration and hybridization, planting the F0 generation hybrid seeds, selecting true hybrid strains, bagging and selfing to obtain F1 generation group mixed seeds, planting F1 generation mixed seeds, and selectively breeding the forage grass type BMR sorghum maintainer line by adopting a pedigree method; in the pedigree method breeding, each generation is selected according to the following conditions: selecting brown midrib; the plant height is 140-160 cm; the total number of the leaves is not less than 30; the number of effective tillers of a single plant is 2-4; the height difference between the tillering stem and the main stem is not more than 5cm, and the flowering phase difference is not more than 7 days; the property of the spike is good, and the spike length is 25-30 cm; tightening the spike shape; selfing the single plants with the maturing rate of more than 98%, and harvesting the single plants when the single plants are mature;
(2) transformation of grass-fed BMR sorghum sterile line
Carrying out continuous backcross transformation on the grain sorghum sterile line as a female parent and the forage grass type BMR sorghum maintainer line bred in the step (1) as a male parent to obtain a forage grass type BMR sorghum sterile line; wherein, the backcross female parent of each generation selects brown midrib, and the complete sterile single plant with other agronomic characters consistent with the forage BMR sorghum maintainer line meets the following conditions: the plant height is 140-160 cm; the total number of the leaves is not less than 30; the number of effective tillers of a single plant is 2-4; the height difference between the tillering stem and the main stem is not more than 5cm, and the flowering phase difference is not more than 7 days; the ear length is 25-30 cm, and the ear shape is medium and tight, and the plant is completely sterile.
Further, in the step (1), the male parent is a forage grass type sorghum maintainer line with more than 2 effective tillers per plant.
Further, the F1 generation mixed seeds are planted, the forage type BMR sorghum maintainer line is bred by a pedigree method, at least 5 generations of pedigree selection is carried out in a mode of 'northern spring sowing planting-southern generation-added generation planting', and each generation is respectively marked and marked independently.
Further, the F1 generation mixed seed is planted, the forage BMR sorghum maintenance line is bred by a pedigree method, and the pedigree selection of F2 generation-F6 generation is carried out by adopting a mode of 'northern spring sowing planting-southern generation-added planting':
selection generation F2: planting the F1 mixed seeds by single-seed dibbling under the northern spring sowing condition, wherein the row spacing is 50cm, and the plant spacing is 20 cm; when planting, the population number is not less than 1000, selecting individual plants according to the conditions in the step (1) for selfing, and carrying out listing and individual marking to obtain F2 generation individual plant seeds;
selection generation F3: selecting the added generations of south propagation, namely planting single seeds of F2 generations by single seed dibbling, wherein the row spacing is 50cm, and the plant spacing is 20 cm; when planting, the population number is not less than 500, selecting individual plants according to the conditions in the step (1) for selfing, and carrying out listing and individual marking to obtain F3 generation individual plant seeds;
selection from generation F4 to generation F6: repeatedly carrying out 'northern spring sowing planting-southern propagation generation-added planting' to continuously complete the breeding of 3 generations, wherein seeds of each generation are planted by single-seed dibbling, the row spacing is 50cm, and the plant spacing is 20 cm; and (3) during planting, selecting single plants according to the conditions in the step (1) for selfing, carrying out listing and individual marking, and obtaining the grass-feeding BMR sorghum maintainer line after F6 generation selection, wherein the population number is not less than 300.
Further, in the step (2), a grain sorghum sterile line is used as a female parent, the forage grass type BMR sorghum maintainer line obtained in the step (1) is used as a male parent, the female parent and the male parent are planted adjacently, the number of female parent groups is not less than 300, the number of male parent groups is not less than 300, the row spacing is 50cm, and the plant spacing is 20 cm; when the flowers bloom, the hybridization is carried out in pairs, and the selfing is carried out on the male parents at the same time; after maturation, BC1 seeds and selfed male parent seeds were harvested in pairs.
Further, in the continuous backcross, backcross female parents and transfer male parents are planted adjacently, the number of female parent populations is not less than 500, the number of transfer male parent populations is not less than 300, the row spacing is 50cm, the plant spacing is 20cm, and the transfer male parents are male parent seeds obtained by selfing the male parents in the previous round of backcross.
Further, the number of backcrosses for the successive backcrosses in step (2) is at least 5 generations.
Further, in the step (1), the determination of true hybrid is carried out by using a sorghum maintainer line of female parent BMR genotype as a contrast, F0 generation hybrid seeds are planted in a single-seed dibble mode, and meanwhile, the F0 generation hybrid seeds are planted adjacent to the female parent of the sorghum maintainer line of the BMR genotype, the row spacing is 50cm, and the plant spacing is 20 cm.
The invention has the beneficial effects that:
successfully breeds the forage grass type BMR sorghum sterile line, has important significance for breeding BMR genotype Gaodan grass new species and improving the feeding quality thereof, and has wide application prospect.
1) The selection standard and the assembly principle of the forage grass type BMR sorghum maintainer parent are determined:
the parent of the parents should be a BMR genotype sorghum maintainer line and the father should be a tillering forage type sorghum maintainer line. When in composition, the broomcorn maintainer line with BMR genotype is taken as a female parent, the forage grass type broomcorn maintainer line with tillering property is taken as a male parent, and hybrid seeds are obtained through castration and hybridization.
2) And innovating a selection standard of the forage grass type BMR sorghum maintainer line field agronomic character phenotype:
selecting brown midrib; the plant height is 140-160 cm; the total number of the leaves is not less than 30; the tillering performance is high, and the effective tillering number of a single plant is 2-4; the height difference between the tillering stem and the main stem is not more than 5cm, and the flowering phase difference is not more than 7 days; the property of the spike is good, and the spike length is 25-30 cm; tightening the spike shape; the BMR single plant with large pollen amount and more than 98 percent of maturing rate is self-bred.
3) The population numbers at the time of selection of the F2 generation of the forage BMR sorghum maintainer line are specified:
as BMR characters are controlled by recessive genes, BMR single plants are selected and meet the forage grass type standard, the population quantity must be increased, and the population quantity is not less than 1000 plants when the forage grass type BMR sorghum maintainer line F2 generation selection is regulated.
4) Providing a selection standard of the forage grass type BMR sorghum sterile line field agronomic character phenotype:
selecting a brown midrib, wherein other agronomic characters are consistent with a forage grass type BMR sorghum maintainer line, and the method mainly comprises the following steps: the plant height is 140-160 cm; the total number of the leaves is not less than 30; the number of effective tillers of a single plant is 2-4; the height difference between the tillering stem and the main stem is not more than 5cm, and the flowering phase difference is not more than 7 days; the ear length is 25-30 cm, and the ear shape is medium and tight, and the plant is completely sterile.
5) Creating a forage grass type BMR sorghum sterile line breeding technical process:
selection of hybrid parents (using a sorghum maintainer line with a BMR genotype as a female parent and using a forage type sorghum maintainer line with more than 2 tillers per plant as a male parent), selection of the forage type BMR sorghum maintainer line (genealogy method), breeding of the forage type BMR sorghum sterile line (continuous backcross method), and formation of the forage type BMR sorghum sterile line.
Detailed Description
The invention innovatively forms a breeding method of forage type BMR sorghum sterile line, and the invention is made by combining the specific embodimentFurther, BMR sorghum maintainer line HZ134B, HG5B sorghum with tillering property and common grain sorghum sterile line A used in the invention3The three feet, three A and the common grain sorghum sterile line HZ214A are provided by the research institute of dry farming agriculture of academy of agriculture and forestry, Hebei province.
(1) Breeding of forage grass type BMR sorghum maintainer line
The F0 generation group: spring 2010, at a dry farming water-saving test station of academy of agriculture and forestry, province of Hebei, where Hebei is in equilibrium with water. Taking BMR sorghum maintainer line HZ134B as a female parent and HG5B sorghum maintainer line with tillering property introduced into Africa as a male parent, manually castrating and hybridizing to obtain F0 generation hybrid seeds, harvesting and storing.
Selection generation F1: in 2010, winter was conducted at a southern trial station of the academy of agriculture and forestry, provincial, Hebei, located in Hainansai san. Single-seed dibbling planting of F0 hybrid seeds, wherein the population number is 500; simultaneously, the BMR female parent is planted adjacently, the row spacing is 50cm, and the plant spacing is 20 cm; through the identification of the success rate of F1 hybrid population, 8F 1 true hybrid plants with comprehensive agronomic characters superior to those of female parents are selected in the heading stage, selfed, bagged and harvested in a mixed mode during maturation to obtain F1 generation mixed seeds.
Selection generation F2: in 2011, spring is carried out at a dry farming water-saving test station of academy of agriculture and forestry, province of Hebei, which is located in Hebei and is capable of balancing water. Single-seed dibbling planting of the F1 generation mixed seeds, wherein the row spacing is 50cm, and the plant spacing is 20 cm; the population number is 1000. Individuals were selected for inbreeding at the F2 generation according to the selection criteria of Table 1. Namely: selecting brown midrib; the total number of the leaves is not less than 30; the number of effective tillers of a single plant is 2-4; the height difference between the tillering stem and the main stem is not more than 5cm, and the flowering phase difference is not more than 7 days; the property of the spike is good, and the spike length is 25-30 cm; tightening the spike shape; and (3) selfing BMR single plants with the maturing rate of more than 98%, individually marking, and harvesting in a mixed mode when the BMR single plants are mature to obtain F2-generation single plant seeds.
Selection generation F3: in 2011, the method is carried out in the southern test station of agriculture and forestry academy of sciences of Hebei province, located in Hainan san. Single-seed dibbling planting is carried out on F2 generation single-plant seeds, the row spacing is 50cm, and the plant spacing is 20 cm; the population number is 500; selection in generation F3 was performed according to the selection criteria in generation F2, and selection continued in its progeny segregating population according to the criteria in table 1: brown midrib; the total number of the leaves is not less than 30; the number of effective tillers of a single plant is 2-4; the height difference between the tillering stem and the main stem is not more than 5cm, and the flowering phase difference is not more than 7 days; the property of the spike is good, and the spike length is 25-30 cm; tightening the spike shape; and (4) selfing the BMR single plants with the maturing rate of more than 98%. At maturity, the individual plants were harvested to give F3 generation individual seeds.
Selection generation F4: spring 2012, which is a dry farming water-saving test station located in north Hebei province academy of agriculture and forestry, where water is balanced. Single-seed dibbling planting is carried out on F3 generation single-plant seeds, the row spacing is 50cm, and the plant spacing is 20 cm; the population number is 300. The F4 generation individuals were selected for selfing according to the selection criteria in Table 1. Individually labeled, and harvested at maturity in a mixed manner to obtain F4 generation individual seeds.
Selection generation F5: in 2012, winter, the method is continued at a southern test station of agriculture and forestry academy of sciences of Hebei province, located in Hainan san. Single-seed dibbling planting is carried out on F4 generation single-plant seeds, the row spacing is 50cm, and the plant spacing is 20 cm; the population number is 300; the selection of F5 generation individuals was continued to self according to the selection criteria in Table 1. And (4) marking separately, harvesting and storing to obtain F5 generation individual seeds.
Selection generation F6: in 2013, the method is carried out in a dry farming water-saving test station of academy of agriculture and forestry, academy of sciences, Hebei province, which is located in Hebei and is capable of balancing water. Single-seed dibbling planting is carried out on F5 generation single-plant seeds, the row spacing is 50cm, and the plant spacing is 20 cm; the population number is 300. The F6 generation individuals were selected for selfing according to the selection criteria in Table 1. And (3) marking the seeds individually, and harvesting the seeds in a mixed manner at the mature stage to obtain F6 generation individual seeds, namely to obtain the stable forage BMR sorghum maintainer line BMR 183.
(2) Backcross transformation of forage BMR sorghum sterile line
Backcross for 1 generation: in 2013, the method is carried out in a southern breeding test station of agriculture and forestry academy of sciences of Hebei province, Hainansan, and uses the sterile line A of the common grain sorghum3Using three feet and three A as female parents, using the forage grass type BMR sorghum maintainer line BMR183 bred in the step (1) as male parents, and carrying out single seed dibbling planting with the row spacing of 50cm and the plant spacing of 20 cm; the number of the father and mother population is 300 strains respectively. During flowering, selecting 3 ears of a male parent and a female parent which are normal in development and typical in plant, carrying out backcross transformation in pairs, simultaneously selfing single plants of the grass-feeding BMR183 sorghum maintainer line BMR183, harvesting and storing in pairs after maturation, and obtaining BC1 seeds and BMR183 selfing 1-generation seeds.
Backcross for 2 generations: in 2014, in the spring, in a dry farming water-saving test station of academy of agriculture and forestry, Hebei province, BC1 seeds and BMR183 inbred 1-generation seeds are planted adjacently, single-seed dibble seeding planting is adopted, the row spacing is 50cm, and the plant spacing is 20 cm. After the BC1 seeds grow, sprout and flower, selecting BMR single plants of completely sterile plants as female parents, using BMR183 inbred 1-generation seeds as male parents for pairwise backcross, simultaneously inbred the BMR183 inbred 1-generation seeds, wherein the number of female parent groups is 500, and the number of male parent groups is 300; after maturation, BC2 seeds and BMR183 inbred 2-generation seeds are harvested from a single plant and stored in pairs.
Backcrossing for 3 generations: in 2014 winter, the method is carried out at a southern breeding test station of agriculture and forestry academy of sciences in Hebei province, BC2 seeds and BMR183 inbred 2-generation seeds are adjacently planted, single-seed dibble planting is adopted, the row spacing is 50cm, the plant spacing is 20cm, when the BC2 seeds grow and flower, single plants which are completely sterile plants and have other agronomic characters consistent with that of a forage grass type BMR sorghum maintainer line BMR183 are selected as female parents, the BMR183 inbred 2-generation seeds are used as male parents for backcrossing, meanwhile, the BMR183 inbred 2-generation seeds are inbred, the number of female parent groups is 500, the number of male parent groups is 300, BC3 seeds and BMR183 inbred 3-generation seeds are obtained.
Backcrossing for 4 generations: in 2015, spring is carried out at a dry farming water-saving test station of academy of agriculture and forestry, Hebei province, BC3 seeds and BMR183 inbred 3-generation seeds are planted adjacently, single-seed dibble planting is adopted, the row spacing is 50cm, and the plant spacing is 20 cm. After the BC3 seeds grow, sprout and flower, selecting a single plant which is completely sterile and has other agronomic characters consistent with the forage grass type BMR sorghum maintainer line BMR183 as a female parent, using BMR183 self-bred 3 generation seeds as male parents for pairwise backcross, and simultaneously using BMR183 self-bred 3 generation seeds for selfing, wherein the number of the female parent population is 500, and the number of the male parent population is 300; after maturation, BC4 seeds and BMR183 inbred 4-generation seeds are harvested from a single plant and stored in pairs.
Backcrossing for 5 generations: in 2015, in a south breeding test station of academy of agriculture and forestry, Hebei province, BC4 seeds and BMR183 inbred 4-generation seeds are planted adjacently, single-seed dibble seeding planting is adopted, the row spacing is 50cm, and the plant spacing is 20 cm. When the BC4 seeds grow, sprout and flower, selecting a single plant which is completely sterile and has other agronomic characters consistent with the forage grass type BMR sorghum maintainer line BMR183 as a female parent, using BMR183 inbred 4-generation seeds as a male parent for pairwise backcross, and simultaneously inbreeding the BMR183 inbred 4-generation seeds, wherein the number of the female parent population is 500, and the number of the male parent population is 300; and harvesting the single plants to obtain BC5 seeds and BMR183 selfing 5-generation seeds, and storing the seeds in pairs.
Backcrossing for 6 generations: in 2016, the method is carried out at a dry farming water-saving test station of academy of agriculture and forestry, Hebei province, wherein BC5 seeds and BMR183 selfing 5-generation seeds are planted adjacently, single-seed dibbling planting is adopted, the row spacing is 50cm, and the plant spacing is 20 cm. After the BC5 seeds grow, sprout and flower, selecting a single plant which is completely sterile and has other agronomic characters consistent with the forage grass type BMR sorghum maintainer line BMR183 as a female parent, using BMR183 inbred 5-generation seeds as male parents for pairwise backcross, and simultaneously inbred the BMR183 inbred 5-generation seeds, wherein the number of the female parent population is 500, and the number of the male parent population is 300; after maturation, BC6 seeds and BMR183 inbred 6-generation seeds are harvested from a single plant and stored in pairs.
Backcrossing for 7 generations: in 2016, the method is carried out continuously at a south propagation test station of academy of agriculture and forestry, Hebei province, BC6 seeds and BMR183 selfing 6 generations of seeds are planted adjacently, single-seed dibbling planting is adopted, the row spacing is 50cm, and the plant spacing is 20 cm. After the BC6 seeds grow, sprout and flower, selecting a single plant with complete sterile plant and other agronomic characters consistent with the forage grass type BMR sorghum maintainer line BMR183 as a female parent, and using BMR183 inbred 6 generation seeds as male parents to carry out paired backcross, wherein the number of the female parent population is 500, the number of the male parent population is 300, and simultaneously the BMR183 inbred 6 generation seeds are inbred to finally obtain the forage grass type BMR sorghum sterile line BMR183A and a preservation line BMR183B thereof. Thus, the new forage BMR sorghum sterile line is successfully transferred.
The forage BMR sorghum sterile line BMR183A is mainly characterized in that: under the condition of Hebei balanced water spring sowing, the growth period is 124 days, the plant height is 155cm, the total number of leaves is 30, the tillering performance is strong, the number of tillers of a single plant is 2-4, the ear length is 28cm, the ear type cylinder is tight in the ear shape, the pollen amount is large, the maturing rate is more than 98%, the regeneration speed is high, and 3-5 regenerated stems can germinate after being cut for 4-6 days; round seeds, white grains with black shells. The main characteristics of the forage BMR sorghum sterile line BMR183A and the common grain sorghum sterile line HZ214A are compared and shown in Table 2.
TABLE 1 selection criteria for field phenotype of grassed BMR sorghum maintainer lines
TABLE 2 Primary characteristic features of the forage BMR sorghum sterile line
Claims (8)
1. A breeding method of forage grass type BMR sorghum sterile line comprises the following steps of carrying out biparental hybridization, pedigree breeding and continuous backcross to obtain the forage grass type BMR sorghum sterile line:
(1) breeding a forage type BMR sorghum maintainer line by taking a BMR genotype sorghum maintainer line as a female parent and taking a forage type sorghum maintainer line with tillerability as a male parent, obtaining F0 generation hybrid seeds through castration hybridization, planting F0 generation hybrid seeds, selecting true hybrid strains, bagging for selfing to obtain F1 generation group mixed seeds, planting F1 generation mixed seeds, and selectively breeding the forage type BMR sorghum maintainer line by adopting a pedigree method; in the pedigree method breeding, each generation is selected according to the following conditions: selecting brown midrib; the plant height is 140-160 cm; the total number of the leaves is not less than 30; the number of effective tillers of a single plant is 2-4; the height difference between the tillering stem and the main stem is not more than 5cm, and the flowering phase difference is not more than 7 days; the property of the spike is good, and the spike length is 25-30 cm; tightening the spike shape; selfing the single plants with the maturing rate of more than 98%, and harvesting the single plants when the single plants are mature;
(2) carrying out continuous backcross transformation on the forage type BMR sorghum sterile line bred in the step (1) by taking the grain sorghum sterile line as a female parent and taking the forage type BMR sorghum maintainer line as a male parent to obtain the forage type BMR sorghum sterile line; wherein, the backcross female parent of each generation selects brown midrib, and the complete sterile single plant with other agronomic characters consistent with the forage BMR sorghum maintainer line meets the following conditions: the plant height is 140-160 cm; the total number of the leaves is not less than 30; the number of effective tillers of a single plant is 2-4; the height difference between the tillering stem and the main stem is not more than 5cm, and the flowering phase difference is not more than 7 days; the ear length is 25-30 cm, and the ear shape is medium and tight, and the plant is completely sterile.
2. The breeding method of the forage type BMR sorghum sterile line according to claim 1, wherein in the step (1), the male parent is a forage type sorghum maintainer line with more than 2 effective tillers per plant.
3. The breeding method of the forage BMR sorghum sterile line according to claim 1, wherein the F1 generation mixed seeds are planted, the forage BMR sorghum maintainer line is selected by a pedigree method in a mode of 'northern spring sowing planting-southern generation-added generation planting' for pedigree selection for at least 5 generations, and each generation is respectively marked and individually marked.
4. The breeding method of the forage BMR sorghum sterile line according to claim 1, wherein the F1 generation mixed seeds are planted, and the forage BMR sorghum maintainer line is selected by a pedigree method in a mode of 'northern spring sowing planting-southern propagation and generation-added planting' to perform F2 generation-F6 generation pedigree selection:
selection generation F2: planting the F1 mixed seeds by single-seed dibbling under the northern spring sowing condition, wherein the row spacing is 50cm, and the plant spacing is 20 cm; when planting, the population number is not less than 1000, selecting individual plants according to the conditions in the step (1) for selfing, and carrying out listing and individual marking to obtain F2 generation individual plant seeds;
selection generation F3: selecting the added generations of south propagation, namely planting single seeds of F2 generations by single seed dibbling, wherein the row spacing is 50cm, and the plant spacing is 20 cm; when planting, the population number is not less than 500, selecting individual plants according to the conditions in the step (1) for selfing, and carrying out listing and individual marking to obtain F3 generation individual plant seeds;
selection from generation F4 to generation F6: repeatedly carrying out 'northern spring sowing planting-southern propagation generation-added planting' to continuously complete the breeding of 3 generations, wherein seeds of each generation are planted by single-seed dibbling, the row spacing is 50cm, and the plant spacing is 20 cm; and (3) during planting, selecting single plants according to the conditions in the step (1) for selfing, carrying out listing and individual marking, and obtaining the grass-feeding BMR sorghum maintainer line after F6 generation selection, wherein the population number is not less than 300.
5. The breeding method of the forage type BMR sorghum sterile line according to claim 1, wherein in the step (2), the grain sorghum sterile line is used as a female parent, the forage type BMR sorghum maintainer line obtained in the step (1) is used as a male parent, the female parent and the male parent are planted adjacently, the number of female parent groups is not less than 300, the number of male parent groups is not less than 300, the row spacing is 50cm, and the plant spacing is 20 cm; when the flowers bloom, the hybridization is carried out in pairs, and the selfing is carried out on the male parents at the same time; after maturation, BC1 seeds and selfed male parent seeds were harvested in pairs.
6. The breeding method of the forage grass type BMR sorghum sterile line according to claim 1, wherein in the continuous backcross, backcrossed female parents and transferred male parents are planted adjacently, the number of female parent population is not less than 500, the number of transferred male parent population is not less than 300, the row spacing is 50cm, the plant spacing is 20cm, and the transferred male parents are male parent seeds obtained by selfing the male parents in the previous round of backcross.
7. The breeding method of the forage BMR sorghum sterile line according to claim 1, wherein the number of backcrosses in the continuous backcrossing in the step (2) is at least 5 generations.
8. The breeding method of the forage BMR sorghum sterile line according to claim 1, wherein the determination of the true hybrid in step (1) is performed by using a sorghum maintainer line of female parent BMR genotype as a control, and F0 generation hybrid seeds are planted in a single-seed dibble mode, and are planted adjacently to the female parent of the sorghum maintainer line of the BMR genotype, wherein the row spacing is 50cm, and the plant spacing is 20 cm.
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| CN114223481B (en) * | 2021-12-30 | 2023-03-21 | 河北省农林科学院旱作农业研究所 | Method for judging purity of BMR sorghum photo-thermo sensitive sterile line hybrid |
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