CN113508713A - Method for breeding trichogramma by using eggs of tuber moths of potatoes - Google Patents
Method for breeding trichogramma by using eggs of tuber moths of potatoes Download PDFInfo
- Publication number
- CN113508713A CN113508713A CN202110874590.0A CN202110874590A CN113508713A CN 113508713 A CN113508713 A CN 113508713A CN 202110874590 A CN202110874590 A CN 202110874590A CN 113508713 A CN113508713 A CN 113508713A
- Authority
- CN
- China
- Prior art keywords
- trichogramma
- eggs
- breeding
- tuber moth
- potato
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 235000013601 eggs Nutrition 0.000 title claims abstract description 106
- 241000256618 Trichogramma Species 0.000 title claims abstract description 65
- 238000009395 breeding Methods 0.000 title claims abstract description 45
- 230000001488 breeding effect Effects 0.000 title claims abstract description 37
- 244000061456 Solanum tuberosum Species 0.000 title claims abstract description 33
- 235000002595 Solanum tuberosum Nutrition 0.000 title claims abstract description 33
- 238000000034 method Methods 0.000 title claims abstract description 28
- 235000012015 potatoes Nutrition 0.000 title abstract description 4
- 241001439019 Phthorimaea operculella Species 0.000 claims abstract description 44
- 238000011081 inoculation Methods 0.000 claims abstract description 8
- 238000012258 culturing Methods 0.000 claims description 19
- 239000004576 sand Substances 0.000 claims description 19
- 241000382353 Pupa Species 0.000 claims description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 17
- 235000012907 honey Nutrition 0.000 claims description 11
- 235000013305 food Nutrition 0.000 claims description 8
- 230000017448 oviposition Effects 0.000 claims description 4
- 239000001963 growth medium Substances 0.000 claims description 3
- 230000019617 pupation Effects 0.000 claims description 3
- 230000003071 parasitic effect Effects 0.000 abstract description 13
- 241000607479 Yersinia pestis Species 0.000 abstract description 6
- 238000002360 preparation method Methods 0.000 abstract description 3
- 241000257303 Hymenoptera Species 0.000 description 16
- 241000238631 Hexapoda Species 0.000 description 13
- 239000002390 adhesive tape Substances 0.000 description 11
- 238000012360 testing method Methods 0.000 description 10
- 241000753145 Sitotroga cerealella Species 0.000 description 9
- 238000011161 development Methods 0.000 description 9
- 230000018109 developmental process Effects 0.000 description 9
- 230000032669 eclosion Effects 0.000 description 8
- 238000007605 air drying Methods 0.000 description 7
- 238000002791 soaking Methods 0.000 description 7
- 239000004744 fabric Substances 0.000 description 5
- 238000002955 isolation Methods 0.000 description 5
- 238000007789 sealing Methods 0.000 description 5
- 241000209140 Triticum Species 0.000 description 4
- 235000021307 Triticum Nutrition 0.000 description 4
- 210000003022 colostrum Anatomy 0.000 description 3
- 235000021277 colostrum Nutrition 0.000 description 3
- 230000024241 parasitism Effects 0.000 description 3
- 241000256815 Apocrita Species 0.000 description 2
- 241000255789 Bombyx mori Species 0.000 description 2
- 241000256856 Vespidae Species 0.000 description 2
- 238000005034 decoration Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000012447 hatching Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- 241000256680 Trichogrammatidae Species 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000008144 egg development Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000004209 hair Anatomy 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 230000001902 propagating effect Effects 0.000 description 1
- 230000000384 rearing effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Toxicology (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention provides a method for breeding trichogramma by using tuber moth eggs of potatoes, belonging to the technical field of biological control of agricultural pests. The inoculation is carried out according to a specific bee-egg ratio, and the problems that trichogramma breeding in the prior art is time-consuming, small in quantity and low in parasitic rate are solved. The method for breeding trichogramma by using the potato tuber moth eggs has short duration and large inoculation quantity, the parasitic rate and the emergence rate of the trichogramma obtained by the breeding method are both over 90 percent, the total culture process is about 30 days on average, the total duration is obviously shorter than that of the breeding method in the prior art, the population quantity of the trichogramma in the next year can be rapidly increased, the preparation is made for large-area application and popularization of the trichogramma, and the problem that the temporary demand of the trichogramma market cannot be met due to insufficient supply of intermediate host eggs is solved. Provides more selection opportunities for the propagation and application of trichogramma, and further promotes the large-area popularization and application of the trichogramma.
Description
Technical Field
The invention relates to the technical field of biological control of agricultural pests, in particular to a method for breeding trichogramma by using tuber moth eggs of potatoes.
Background
Trichogramma (trichogram wastpood), Hymenoptera (Hymenoptera), Apocrita (Apocrita), Trichogramma (chalcoidea), Trichogramma, Trichogrammatidae, a parasitic egg bee; the trichogramma bee larva is distributed all over the world and is various, at present, more than 180 trichogramma species are recorded all over the world, and 29 species are recorded in China. The trichogramma has become an important egg parasitic bee with the longest research history, the widest application range, the largest control area and the best pest control effect in the modern agriculture and forestry pest biological control; the agricultural and forestry pests successfully controlled by trichogramma can be more than 28, and the pest control area by releasing trichogramma per year can be 3 multiplied by 107hm2Above, obvious economic, ecological and social benefits are obtained.
At present, hosts such as a wheat moth egg, a rice moth egg, an oak silkworm egg and the like exist as host eggs for large-scale production at home and abroad, and China successfully adopts a technology and a process flow for propagating trichogramma by using the wheat moth egg or the rice moth egg (small egg) and the oak silkworm egg (large egg) in a large scale. However, the feeding period of the wheat moth, the rice moth and the tussah is longer and is more than 50 days, which causes certain influence on the rapid mass propagation of the trichogramma. Even if the emergence time of the trichogramma is different from the time for obtaining the intermediate host egg, the intermediate host egg needs to be stored at low temperature to meet the parasitism of the trichogramma, so that the parasitism rate of the trichogramma is greatly reduced. The egg demand is closely related to the production plan, when the demand is large, the demand can be satisfied after several generations of hosts need to be raised, particularly after the seeds are preserved in winter, the propagation period is started next year and is often limited by the factor, and the defect is more obvious when the demand is large. Therefore, the method is time-consuming, labor-consuming and high in cost. Meanwhile, once the production capacity planned in the beginning of the year is determined, if the dosage is temporarily increased midway, the growth duration of the rice moths and the wheat moths is too long to meet the requirement. The factors limit the large-area application and popularization of the trichogramma to a certain extent.
Disclosure of Invention
The invention aims to provide a method for breeding trichogramma by utilizing potato tuber moth eggs, which solves the problems that the breeding of the trichogramma in the prior art is time-consuming, small in quantity and low in parasitic rate.
In order to achieve the above object, the present invention provides the following technical solutions:
the invention provides a method for breeding trichogramma by utilizing a tuber moth egg, which comprises the following steps:
inoculating trichogramma and potato tuber moth eggs in a bee egg ratio of 1: 5-15, and culturing for 23-26 h after inoculation.
Preferably, the temperature of the culture is 24-26 ℃, the humidity is 70-80%, and the photoperiod is 12-16L: 8-12D.
Preferably, the number ratio of the male peak to the female peak in the trichogramma is 1: 2-4.
Preferably, the culture adopts honey water as a food source, and the concentration of the honey water is 40-60%.
Preferably, the tuber moth egg is obtained by a breeding method comprising the following steps:
s1, larva breeding: inoculating the colostrum larva to potato tubers, and culturing to obtain aged larva;
s2, pupation: culturing the obtained aged larva to obtain pupa;
s3, feathering: further culturing the pupae to obtain adult pupae;
s4, spawning: and continuously culturing the obtained imagoes to the egg-laying period to obtain the potato tuber moth eggs.
Preferably, the temperature for culturing in the step S1 is 25-28 ℃, and the humidity is 70-80%.
Preferably, the population density of the larvae inoculated in the step S1 is 20-260 colostrum larvae per kilogram of potato tubers.
Preferably, the population density of the larvae inoculated in step S1 is 20, 80, 120, 200 or 260 colostrum larvae per kg of potato tuber.
Preferably, the culture medium used in the step S2 is river sand.
Preferably, the temperature for culturing in the step S3 is 25-28 ℃, and the humidity is 70-80%.
The invention has the technical effects and advantages that:
the method for breeding trichogramma by using the potato tuber moth eggs has short duration and large inoculation quantity, the parasitic rate and the emergence rate of the trichogramma obtained by the breeding method are both over 90 percent, the total culture process is about 30 days on average, the total duration is obviously shorter than that of the breeding method in the prior art, the population quantity of the trichogramma in the next year can be rapidly increased, the preparation is made for large-area application and popularization of the trichogramma, and the problem that the temporary demand of the trichogramma market cannot be met due to insufficient supply of intermediate host eggs is solved. Provides more selection opportunities for the propagation and application of trichogramma, and further promotes the large-area popularization and application of the trichogramma.
Detailed Description
The invention provides a method for breeding trichogramma by utilizing a tuber moth egg, which comprises the following steps:
inoculating trichogramma and potato tuber moth eggs in a bee egg ratio of 1: 5-15, and culturing for 23-26 h after inoculation.
The trichogramma and potato tuber moth eggs are inoculated at a bee-egg ratio of 1: 5-15, preferably at a bee-egg ratio of 1: 7-12, and more preferably at a bee-egg ratio of 1: 9-11.
The culture temperature is preferably 24-26 ℃, and more preferably 25 ℃; the culture humidity is preferably 70-80%, more preferably 74-77%, and still more preferably 75-76%; the culture photoperiod is preferably 12-16L: 8-12D, and more preferably 13-15L: 9-11D.
In the invention, the number ratio of the male peak to the female peak in the trichogramma is preferably 1: 2-4, and more preferably 1: 3. The culture preferably adopts honey water as a food source, and the concentration of the honey water is preferably 40-60%, more preferably 42-48%, and still more preferably 44-46%.
In the present invention, the tuber moth egg is preferably obtained by a rearing method comprising the steps of:
s1, larva breeding: inoculating the tuber moth eggs to potato tubers, and culturing to obtain mature larvae;
s2, pupation: culturing the obtained aged larva to obtain pupa;
s3, feathering: further culturing the pupae to obtain adult pupae;
s4, spawning: and continuously culturing the obtained imagoes to the egg-laying period to obtain the potato tuber moth eggs.
In the present invention, before the inoculation in step S1, soaking the potato tubers in water for 4-6 min is preferably further included, and after the soaking, air drying is preferably included, and after the air drying, small holes are preferably punctured on the surfaces of the potato tubers. The temperature for culturing in the step S1 is preferably 25-28 ℃, and more preferably 26-27 ℃; the humidity of the culture in the step S1 is preferably 70-80%, more preferably 72-78%, and still more preferably 74-75%. The population density of the potato tuber inoculated in the step S1 is preferably 20-260 eggs of the potato tuber moth inoculated to each kilogram of potato tubers, more preferably 40-200 eggs of the potato tuber moth inoculated to each kilogram of potato tubers, and further preferably 70-100 eggs of the potato tuber moth inoculated to each kilogram of potato tubers.
The culture medium used in the culture in step S2 of the present invention is preferably river sand.
The temperature for culturing in the step S3 is preferably 25-28 ℃, and more preferably 26-27 ℃; the humidity of the culture in the step S3 is preferably 70-80%, more preferably 72-78%, and still more preferably 74-75%.
The technical solutions provided by the present invention are described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention.
Example 1
Soaking potato tubers in water for 5min, naturally air-drying at room temperature, piercing small holes on the surfaces of the tubers by using an insect dissecting needle, placing the potato tubers on an isolation net in an insect breeding box, inoculating 100 eggs of potato tuber moths to 5kg of potato tubers, and breeding at the temperature of 25 ℃ and the humidity of 70%; when the mature larvae develop, tubers are drilled out and fall into river sand to pupate; periodically replacing river sand, collecting, separating pupa and river sand by using a sample separating sieve, transferring the obtained pupa into a pupa eclosion box, and developing under the conditions of temperature of 26 ℃ and humidity of 76%; after emerging, the imagoes are transferred to an egg collection box to lay eggs, and the eggs of the potato tuber moths are collected.
Placing the double-sided adhesive tape adhered with the potato tuber moth eggs in a large test tube, transferring the double-sided adhesive tape into newly emerged adult bees according to the bee-egg ratio of 1:10, wherein the number ratio of male peaks to female bees is 1:2, sealing the opening of the test tube by using black cloth, and taking 50% of honey water as a food source. Parasitizing the seeds for 24 hours in a light culture box with the temperature of 24 ℃, the humidity of 75% and the photoperiod of 12L to 12D.
Example 2
Soaking potato tubers in water for 5min, naturally air-drying at room temperature, piercing small holes on the surfaces of the tubers by using an insect dissecting needle, placing the potato tubers on an isolation net in an insect breeding box, inoculating 400 eggs of potato tuber moths to 5kg of potato tubers, and breeding at the temperature of 24 ℃ and the humidity of 75%; when the mature larvae develop, tubers are drilled out and fall into river sand to pupate; periodically replacing river sand, collecting, separating pupa and river sand by using a sample separating sieve, transferring the obtained pupa into a pupa eclosion box, and developing under the conditions of 25 ℃ of temperature and 78% of humidity; after emerging, the imagoes are transferred to an egg collection box to lay eggs, and the eggs of the potato tuber moths are collected.
Placing the double-sided adhesive tape adhered with the potato tuber moth eggs in a large test tube, transferring the double-sided adhesive tape into newly emerged adult bees according to the bee-egg ratio of 1:7, wherein the number ratio of male peaks to female bees is 1:4, sealing the opening of the test tube by using black cloth, and taking 55% of honey water as a food source. Parasitizing the seeds in a light incubator with the temperature of 26 ℃, the humidity of 70% and the photoperiod of 14L to 10D for 24 hours.
Example 3
Soaking potato tubers in water for 5min, naturally air-drying at room temperature, piercing small holes on the surfaces of the tubers by using an insect dissecting needle, placing the potato tubers on an isolation net in an insect breeding box, inoculating 700 eggs of potato tuber moths to 5kg of potato tubers, and breeding at the temperature of 26 ℃ and the humidity of 80%; when the mature larvae develop, tubers are drilled out and fall into river sand to pupate; periodically replacing river sand, collecting, separating pupa and river sand by using a sample separating sieve, transferring the obtained pupa into a pupa eclosion box, and developing under the condition of 27 ℃ of temperature and 77% of humidity; after emerging, the imagoes are transferred to an egg collection box to lay eggs, and the eggs of the potato tuber moths are collected.
Placing the double-sided adhesive tape adhered with the eggs of the potato tuber moth in a large test tube, transferring the double-sided adhesive tape into newly emerged adult bees according to the ratio of the number of the eggs to the number of the newly emerged adult bees of 1:12, wherein the number ratio of the male peaks to the female bees is 1:3, sealing the opening of the test tube by using black cloth, and taking 50% of honey water as a food source. Parasitizing the seeds in a light incubator with the temperature of 25 ℃, the humidity of 75% and the photoperiod of 13L to 11D for 24 hours.
Example 4
Soaking potato tubers in water for 5min, naturally air-drying at room temperature, piercing small holes on the surfaces of the tubers by using an insect dissecting needle, placing the potato tubers on an isolation net in an insect breeding box, inoculating 1000 eggs of potato tuber moths to 5kg of potato tubers, and breeding at the temperature of 26 ℃ and the humidity of 70%; when the mature larvae develop, tubers are drilled out and fall into river sand to pupate; periodically replacing river sand, collecting, separating pupa and river sand by using a sample separating sieve, transferring the obtained pupa into a pupa eclosion box, and developing under the conditions of 25 ℃ of temperature and 80% of humidity; after emerging, the imagoes are transferred to an egg collection box to lay eggs, and the eggs of the potato tuber moths are collected.
Placing the double-sided adhesive tape adhered with the potato tuber moth eggs in a large test tube, transferring the double-sided adhesive tape into newly emerged adult bees according to the bee-egg ratio of 1:10, wherein the number ratio of male peaks to female bees is 1:2, sealing the opening of the test tube by using black cloth, and taking 45% of honey water as a food source. Parasitizing the seeds for 24 hours in a light culture box with the temperature of 24 ℃, the humidity of 75% and the photoperiod of 15L to 9D.
Example 5
Soaking potato tubers in water for 5min, naturally air-drying at room temperature, piercing small holes on the surfaces of the tubers by using an insect dissecting needle, placing the potato tubers on an isolation net in an insect breeding box, inoculating 1300 pieces of potato tuber moth eggs on 5kg of potato tubers, and breeding at the temperature of 26 ℃ and the humidity of 70%; when the mature larvae develop, tubers are drilled out and fall into river sand to pupate; periodically replacing river sand, collecting, separating pupa and river sand by using a sample separating sieve, transferring the obtained pupa into a pupa eclosion box, and developing under the conditions of 26 ℃ of temperature and 71% of humidity; after emerging, the imagoes are transferred to an egg collection box to lay eggs, and the eggs of the potato tuber moths are collected.
Placing the double-sided adhesive tape adhered with the potato tuber moth eggs in a large test tube, transferring the double-sided adhesive tape into newly emerged adult bees according to the bee-egg ratio of 1:14, wherein the number ratio of male peaks to female bees is 1:2, sealing the opening of the test tube by using black cloth, and taking 55% of honey water as a food source. Parasitizing the seeds in a light culture box with the temperature of 24 ℃, the humidity of 80% and the photoperiod of 16L to 8D for 24 hours.
Experimental example 1
The examples 1 to 5 were recorded as an experimental group A, B, C, D, E, and the following indexes were measured and recorded during the implementation process, and the procedure was as follows:
when the potato is raised in a larva raising box for 10 days, randomly drawing 30 potato tubers, splitting the tubers to see whether the larvae of the potato tuber moth exist, if yes, indicating that the larvae of the potato tuber moth eat the tubers, and otherwise, indicating that the larvae of the potato tuber moth do not eat the tubers. When the aged larvae pupate, observing and recording the total amount of pupae in the river sand to calculate the survival rate of the larvae. Observing and recording the total amount of eclosion of the adult insects, and calculating the eclosion rate of the adult insects. Each treatment was repeated 3 times. The calculation formula is as follows:
30 eggs are randomly placed on the filter paper respectively, and the hatching condition of the eggs is observed and recorded. And calculating the development period and the hatching rate of the eggs.
Collecting and recording the number of the male and female adults, and calculating the male and female proportion. The egg laying amount is collected and recorded by a purifying device. Randomly collecting 30 eggs of the phaeomysis hubner from the collected eggs, placing the eggs under an insect dissecting mirror, and measuring the length and the width of the eggs by using a vernier caliper. Repeat 3 times.
Parasitic ratio: after 7 days of parasitic egg development, the egg-carrying double-sided adhesive tape was removed and placed under a field of view of a dissecting mirror (eyepiece × objective lens ═ 10 × 5), and the number of black (parasitic egg), white and shriveled (non-parasitic egg) eggs was checked and recorded, and the parasitic rate was calculated. Repeat 3 times.
Eclosion rate: after the trichogramma had emerged, the double-sided tape was taken out and placed under a field of view of a dissecting mirror (eyepiece × objective lens ═ 10 × 5), the number of black eggs was recorded, and it was examined whether there were any emerged holes thereon, and if there were no eggs with obvious emerged holes, it was picked up with a pin, and it was examined whether there were any parasitic wasp residues or no emerged adult wasps therein, which were shown as emerged if there were any, and which were not emerged if there were any liquid emerging from the eggs. The emergence rate is calculated. Repeat 3 times.
The female bee ratio is calculated by randomly selecting 30 adult bees, placing under the field of view of a dissecting mirror (eyepiece x objective lens is 10 x 5), checking and recording the number of female bees (the tentacle minor is in a rod shape) and male bees (in a rod shape and growing hairs).
The statistical results are shown in table 1.
TABLE 1
Therefore, the tuber moth eggs cultured by the embodiment of the application have high hatchability and short development time, and the parasitic rate and the emergence rate of the tuber moth eggs obtained from the experimental groups A to E are over 90 percent when the tuber moth eggs are used for breeding trichogramma. However, each group showed a difference in development duration, taking the development duration as an example, wherein the longest development duration is the tuber moth in group E, which is different from the tuber moths in other groups 4, and the next is the development durations of the tuber moths in groups C and D, which are not different from the development durations of the tuber moths in other groups 3. The shortest is the development period of the potato tuber moth in the group A, which is different from the other 4 groups. The tuber moth eggs with the largest quantity and reliable quality can be obtained by breeding the tuber moth under the conditions of the experimental group B and the experimental group C by comprehensively considering the factors of the development history, the survival rate of larvae, the quantity of female moths produced, the quantity of eggs laid, the sex ratio and the size of eggs, and the trichogramma wasps can be bred to the maximum extent.
Experimental example 2
The performance analysis was performed with example 3 using the trichogramma bred from the eggs of the rice moth as a control group in the conventional technical means, and the results showed that: the average time to obtain the rice moth eggs is 50 days, and the average time to obtain the potato tuber moth eggs is 24 days. The first generation of rice moth is bred, each kilogram of feed can obtain 33.6 ten thousand rice moth eggs, and in the same time period, the potato tuber moth in the example 3 can be propagated for 2 generations, so that 9929.67 ten thousand eggs which are 295.25 times of rice moth eggs can be obtained.
As can be seen from the above examples, the present invention provides a method for breeding trichogramma using eggs of potato tuber moth for a short period of time and a large number of inoculations. The trichogramma parasitism rate and the emergence rate obtained by the breeding method are both over 90 percent, the total culture process is about 30 days on average, the total duration is obviously shorter than that of the breeding method in the prior art, the population quantity of the trichogramma in the next year can be rapidly increased, preparation is made for large-area application and popularization of the trichogramma, and the problem that the temporary demand of the trichogramma market cannot be met due to insufficient supply of intermediate host eggs is solved. Provides more selection opportunities for the propagation and application of trichogramma, and further promotes the large-area popularization and application of the trichogramma.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (10)
1. A method for breeding trichogramma by utilizing a potato tuber moth egg is characterized by comprising the following steps:
inoculating trichogramma and potato tuber moth eggs in a bee egg ratio of 1: 5-15, and culturing for 23-26 h after inoculation.
2. The method for breeding trichogramma by using the eggs of the potato tuber moth as claimed in claim 1, wherein the cultivation temperature is 24-26 ℃, the humidity is 70-80%, and the photoperiod is 12-16L: 8-12D.
3. The method for breeding trichogramma by utilizing the eggs of the potato tuber moth as claimed in claim 2, wherein the number ratio of male peaks to female peaks in the trichogramma is 1: 2-4.
4. The method for breeding trichogramma by using the eggs of the potato tuber moth as claimed in claim 2, wherein the cultivation adopts honey water as a food source, and the concentration of the honey water is 40-60%.
5. The method for breeding trichogramma as claimed in claim 1, wherein the eggs of the tuber moth are obtained by a breeding method comprising the steps of:
s1, larva breeding: inoculating the tuber moth eggs to potato tubers, and culturing to obtain mature larvae;
s2, pupation: culturing the obtained aged larva to obtain pupa;
s3, feathering: further culturing the pupae to obtain adult pupae;
s4, spawning: and continuously culturing the obtained imagoes to the egg-laying period to obtain the potato tuber moth eggs.
6. The method for breeding trichogramma by using the eggs of the potato tuber moth as claimed in claim 5, wherein the temperature of the culture in the step S1 is 25-28 ℃, and the humidity is 70-80%.
7. The method for breeding trichogramma by using the eggs of the potato tuber moth as claimed in claim 6, wherein the population density of the eggs inoculated in the step S1 is 20-260 eggs of the potato tuber moth per kilogram of potato tubers.
8. The method for breeding trichogramma as claimed in claim 7, wherein the population density of the eggs inoculated in step S1 is 20, 80, 120, 200 or 260 eggs of potato tuber moth per kg of potato tuber moth.
9. The method for breeding trichogramma as claimed in claim 8, wherein the culture medium used in the step S2 is river sand.
10. The method for breeding trichogramma as claimed in claim 9, wherein the temperature of the cultivation in step S3 is 25-28 ℃ and the humidity is 70-80%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110874590.0A CN113508713A (en) | 2021-07-30 | 2021-07-30 | Method for breeding trichogramma by using eggs of tuber moths of potatoes |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110874590.0A CN113508713A (en) | 2021-07-30 | 2021-07-30 | Method for breeding trichogramma by using eggs of tuber moths of potatoes |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113508713A true CN113508713A (en) | 2021-10-19 |
Family
ID=78068922
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110874590.0A Pending CN113508713A (en) | 2021-07-30 | 2021-07-30 | Method for breeding trichogramma by using eggs of tuber moths of potatoes |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113508713A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114403097A (en) * | 2021-11-19 | 2022-04-29 | 贵州大学 | Breeding device and breeding method for pyemotes zhonghuajia |
| CN116171941A (en) * | 2023-03-08 | 2023-05-30 | 福建省农业科学院水稻研究所 | Application of wheat seedlings in cold storage of trichogramma longsynanthassemblies and cold storage method |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105145487A (en) * | 2014-07-23 | 2015-12-16 | 中国科学院动物研究所 | Trichogramma japonicun ashmead artifical breeding production method |
| CN106614396A (en) * | 2016-12-02 | 2017-05-10 | 中国农业科学院植物保护研究所 | Method for feeding sitotroga cerealella by utilizing unhusked rice |
| CN108575920A (en) * | 2018-07-05 | 2018-09-28 | 中国农业科学院植物保护研究所 | A kind of breeding apparatus and method for breeding of phthorimaea operculella |
-
2021
- 2021-07-30 CN CN202110874590.0A patent/CN113508713A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105145487A (en) * | 2014-07-23 | 2015-12-16 | 中国科学院动物研究所 | Trichogramma japonicun ashmead artifical breeding production method |
| CN106614396A (en) * | 2016-12-02 | 2017-05-10 | 中国农业科学院植物保护研究所 | Method for feeding sitotroga cerealella by utilizing unhusked rice |
| CN108575920A (en) * | 2018-07-05 | 2018-09-28 | 中国农业科学院植物保护研究所 | A kind of breeding apparatus and method for breeding of phthorimaea operculella |
Non-Patent Citations (8)
| Title |
|---|
| G. SAOUR: "Parasitization of potato tuber moth eggs (Lep., Gelechiidae) from irradiated adults by Trichogramma (Hym., Trichogrammatidae) and control of moth population with combined releases of sterile insect and egg parasitoid", 《JOURNAL OF APPLIED ENTOMOLOGY》 * |
| RAMI KFIR: "Functional response to host density by the egg parasiteTrichogramma pretiosum", 《ENTOMOPHAGA》 * |
| 杨榅: "《赤眼蜂及其利用》", 31 August 1979, 内蒙古人民出版社 * |
| 桂富荣等: "用马铃薯人工饲养马铃薯块茎蛾的方法", 《昆虫知识》 * |
| 沈健等: "暗黑赤眼蜂对梨小食心虫卵的寄生作用", 《植物保护学报》 * |
| 闫俊杰等: "马铃薯块茎蛾生物学、生态学与综合治理", 《昆虫学报》 * |
| 阜南县赤眼蜂研究所: "《赤眼蜂的研究与利用》", 30 April 1976, 安徽人民出版社 * |
| 马艳粉等: "幼虫密度对马铃薯块茎蛾生长发育及繁殖的影响", 《昆虫知识》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114403097A (en) * | 2021-11-19 | 2022-04-29 | 贵州大学 | Breeding device and breeding method for pyemotes zhonghuajia |
| CN116171941A (en) * | 2023-03-08 | 2023-05-30 | 福建省农业科学院水稻研究所 | Application of wheat seedlings in cold storage of trichogramma longsynanthassemblies and cold storage method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102578052B (en) | A bayberry orchard Drosophila elanogaster artificial breeding method, and method for breeding parasitic wasp with the same | |
| CN108056070B (en) | Method for breeding trichogramma by using snowflake moth | |
| CN1631127A (en) | Artificial progagation method for ladybird beetle and lacewing fly | |
| CN108496900B (en) | Breeding method and using method for preventing and treating lygus tridentatus for moringa oleifera naotus | |
| CN102125002A (en) | Method for artificially raising plant bug parasitic wasps | |
| CN101849529A (en) | Method for breeding high-quality anti-mite bee variety | |
| CN111328770B (en) | Indoor large-scale breeding method for nakayata nakamura | |
| Palafox-Luna et al. | Life cycle and fecundity of Dactylopius opuntiae (Hemiptera: Dactylopiidae) in Opuntia ficus-indica (Caryophyllales: Cactaceae) | |
| CN103109781A (en) | Artificial breeding method of Taiwan abdominal cocoon bee | |
| CN105746435A (en) | Method for propagating chrysopa pallens larvae through rice moth eggs | |
| CN112042598B (en) | Whole set method for breeding harmonia axyridis | |
| CN101904320A (en) | Method for breeding a large number of microplitis bicoloratus | |
| CN108012995A (en) | A kind of indoor feeding method and device of green wing thin,tough silk open country snout moth's larva | |
| CN113349166B (en) | Method for breeding rice louse trichogramma in batches | |
| CN113508713A (en) | Method for breeding trichogramma by using eggs of tuber moths of potatoes | |
| Ramzan et al. | Biology of fall armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae) a new alien invasive Pest in Pakistan | |
| CN111685083A (en) | Method for raising wheat moths by using wheat | |
| CN114651793B (en) | Artificial breeding of trichogramma obscura and method for preventing and treating spodoptera frugiperda by using trichogramma obscura | |
| CN115428770A (en) | Method for adjusting egg laying time of ladybug by using alternative feed | |
| CN111543396B (en) | Indoor large-scale breeding method for small flies and pupa terracotta warriors | |
| Ogah et al. | Parasitism and development of Platygaster diplosisae (Hymenoptera: Platygastridae) on the African rice gall midge Orseolia oryzivora (Diptera: Cecidomyiidae) | |
| CN111727936A (en) | Indoor propagation method of chilo suppressalis fringed cocoon bee | |
| CN112471082A (en) | Broad-band optimized aphid fly breeding method | |
| CN105941350A (en) | Rearing method with mulberry shoots for silkworms of all ages | |
| CN114304073B (en) | Artificial feeding and egg collecting method for Trigonella Foenum-graecum |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20211019 |
|
| RJ01 | Rejection of invention patent application after publication |