CN114711198A - Method for breeding Spodoptera frugiperda by wheat hydroponic seedling disk grouping - Google Patents
Method for breeding Spodoptera frugiperda by wheat hydroponic seedling disk grouping Download PDFInfo
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Abstract
The invention discloses a method for breeding spodoptera frugiperda by utilizing wheat water culture seedling plates in a grouping manner, which comprises the steps of preparation of wheat water culture seedling plates, breeding frame construction, breeding of spodoptera frugiperda larvae and the like. The wheat seedlings can be used as foodstuff to feed spodoptera frugiperda larvae, and can also be used as a spawning trapping medium to attract spodoptera frugiperda adults to spawn, the vertical overlapping structure of the seedling trays also ensures that the wheat seedlings in the feeding period are not collapsed and can maintain the characteristics of fresh and alive growth, and the wheat seedling trays are easy to obtain indoors, so that the annual cultivation of fresh feed can be continued.
Description
Technical Field
The invention belongs to the field of biotechnology of agriculture and forestry, relates to a method for artificially feeding spodoptera frugiperda, and particularly relates to a method for groupingly feeding spodoptera frugiperda by utilizing wheat water-cultured seedling trays.
Background
Spodoptera frugiperda belongs to the Spodoptera frugiperda of Lepidoptera Spodoptera, is native to tropical and subtropical regions of America, has the characteristics of omnivory, predatory property, migration property, high reproductive capacity and the like, and is a major dangerous migratory insect which seriously threatens agricultural production. In 2016, 1 month, the fall armyworm inhabiting the continental america was brought by commercial and trade shipments to nigeria on the west coast of africa, and subsequently only 2 years of time was spent panning 44 countries and regions in the south of sahara, which had a great impact on food safety in africa. The international agricultural and biological science center of 2017 lists spodoptera frugiperda as one of ten plant pests in the world. Since 7 months in 2018, Spodoptera frugiperda migrated across the sea into also and India, gradually spreading deep throughout southeast Asian countries.
In 11 days in 2019, 1 month and 11 months in China, Spodoptera frugiperda is found to be harmful to corn for the first time, in 4 months, the Spodoptera frugiperda invades Shuangguang, Guizhou, Hunan and Hainan, and in 5 months, the Spodoptera frugiperda rapidly spreads to Fujian, Hubei, Zhejiang, Sichuan, Jiangxi, Chongqing, Henan and other places. By 10 and 8 months in 2019, Spodoptera frugiperda invades 1518 counties in 26 provinces in China. The corn emergence area in 2019 is about 106.5 kilohm2And causes harm to other crops such as 14 crops of sugarcane, sorghum, millet, wheat and the like. In the rural area of agriculture, the emergence situation of the spodoptera frugiperda is strictly concerned when the spodoptera frugiperda epidemic situation is outbreak in Asian countries and regions, and the monitoring, prevention and control work of the spodoptera frugiperda is highly regarded. The spodoptera frugiperda monitoring and early warning work is arranged and deployed in 12 months in 2018, the national agriculture technical center has printed 'notice on the spodoptera frugiperda invasion hazard prevention work' (Chinese plant protection and periodicals, 2019) in 2019, and the rural part of 6 months in 2019 has printed 'national spodoptera frugiperda prevention and control scheme' in the countryside, so that the precise guidance and careful deployment are made on the aspects of spodoptera frugiperda prevention and control target tasks, monitoring and prevention and control measures and the like in China, so that the spodoptera frugiperda migration hazard in a small scale is ensured in the major maize producing area, the continuous disaster of the maize is ensured, and the overall target task of disaster loss is reduced to the maximum extent.
Along with the development of the constantly deepening of spodoptera frugiperda scientific research and prevention and cure technique, need the insect source that develops unanimously in a large number, physiology is drawn one, and receive season and the restriction of fresh food host natural growth cycle to spodoptera frugiperda larva under higher density, the larva often appears and can only kill each other, or the condition that the larva baits takes place, and the mode container that adopts single larva to raise is many, waste time and energy, complex operation. Therefore, a method for group breeding Spodoptera frugiperda by fresh feed which can be industrially and massively produced in an indoor way and by structurally arranging a pest breeding container, optimizing a breeding method and a space-time flow is urgently needed.
Disclosure of Invention
The invention aims to provide a method for group breeding Spodoptera frugiperda by utilizing wheat water culture seedling discs, which has simple operation process, and is safe and reliable. Under the indoor condition, the array wheat seedlings are cultivated by using seedling trays with fixed specifications in an odd-numbered row sowing mode and an even-numbered row sowing mode, the seedling trays are used as bearing media, the seedling trays perpendicular to the odd-numbered row seedling trays and the even-numbered row seedling trays are mutually opposite and overlapped to form a wheat seedling horizontal direction overlapping feeding structure, a wheat seedling standing upright at high density is created to form a vertical and thick wide three-dimensional space, a light-resistant feeding space is provided, killing behaviors generated by contacting of spodoptera frugiperda larvae with each other are effectively isolated, and the feeding density is improved; meanwhile, sand is provided at the bottom of the wheat seedling tray combined unit for pupating mature larvae of spodoptera frugiperda, so that the pupae can be conveniently collected in the later period; the characteristic that the wheat seedling tray can induce the spodoptera frugiperda adults to lay eggs is also utilized, egg masses can be collected continuously, the limitation that fresh corn leaves are required to be used for feeding spodoptera frugiperda is eliminated, and labor is saved; provides a large number of uniformly developed insect sources for testing for further expanding the research and application of the techniques such as the biological ecology, physiology, toxicology, biological control and the like of the spodoptera frugiperda in the future.
In order to achieve the purpose, the technical scheme of the invention is as follows: the method for group breeding of spodoptera frugiperda by using wheat hydroponic seedling discs comprises the following steps:
step S1, preparing a wheat water culture seedling tray:
dividing the seedling tray into 5 rows of seeding channels according to the width of the seedling tray, taking a certain amount of wheat seeds, after the wheat seeds are soaked in clear water after being washed, sowing the wheat seeds on odd seedling channels (1, 3 and 5 seedling channels) of the first seedling tray, sowing the wheat seeds on even seedling channels (2 and 4 seedling channels) of the second seedling tray to obtain odd seedling trays and even seedling trays, suspending the seedling trays with holes on the water surface for standing for 9-12 days, after green wheat seedlings grow to 15-20cm, taking out the seedling trays, and draining water to obtain the wheat water-cultured seedling tray;
step S2, the structure of the rearing stand:
the feeding frame comprises a bottom empty tray which is horizontally placed and a plurality of wheat water-cultured seedling trays which are vertically placed above the bottom empty tray, the same odd-numbered seedling trays or even-numbered seedling trays are fixed back to form feeding units, the adjacent feeding units are odd-even, so that the opposite wheat water-cultured seedlings are staggered, mutually embedded and oppositely arranged, and the feeding frame with seedling-to-seedling and root-to-root arrangement is formed;
step S3, feeding Spodoptera frugiperda larvae:
step S3-1: quantitatively adding spodoptera frugiperda egg blocks at the top of the wheat water culture seedling tray, wherein the ratio of the number of the eggs to the area density of the wheat seedlings is 1: 0.59-1.7 cm2The hatched larvae eat young wheat seedlings, the larvae automatically disperse and eat after 2-3 years, and the residual seedlings gradually fall into a lower wheat seedling path;
step S3-2: replacing the wheat water culture seedling tray for 3-7 days in the feeding process, cleaning insect manure and broken seedling residues, repeatedly stacking the wheat water culture seedling tray after pulling out fresh wheat seedlings which are not eaten on the wheat water culture seedling tray, putting the larvae falling into an empty tray on the wheat seedlings at the top of the wheat water culture seedling tray, and continuously feeding the larvae until the larvae are mature;
step S3-3: after the larvae grow to 4 years old, the density of the larvae reaches 1 head to 20cm2When the larvae grow to 5-6 years old, the density of the larvae reaches 1 head at 30cm2The larvae are separated in time, so that the larvae can be prevented from contacting with each other after the larvae are 4 years old, and the killing action can be finished;
step S3-4: replacing the bottom empty tray with a sand tray containing sand after the larvae grow to 5-6 years old so that the aged larvae enter the sand to pupate;
step S4, collection of pupae, adult breeding, preservation of eggs and subculture:
s4-1, after pupating the larva to be aged in sand, screening out the larva from the sand, putting the larva into a clean glass bottle, and standing until the pupa eclosion;
s4-2, putting eclosion adults into an insect cage, placing a wheat seedling tray at the bottom of the insect cage for inducing the adults to lay eggs, and hanging cotton balls dipped with honey water for supplementing nutrition;
and S4-3, replacing cotton balls and collecting egg blocks every day, pulling out wheat seedlings with the egg blocks, or putting the collected spodoptera frugiperda egg blocks into a refrigerator at 5 ℃ for storage for 2 days, taking out the wheat seedlings when the wheat seedlings are used, or directly placing the wheat seedlings in the wheat water culture seedling tray of the step S3, and circularly propagating and breeding the wheat seedlings.
Further, in step S1, the wheat seeds are soaked in clear water for 8-14 h.
Furthermore, wheat seeds can be purchased in the market, and the yield and the quality of the seeds are not required.
Further, in the step S1, the sowing width of the wheat seeds is 4-10 cm.
Further, in step S1, seedling trays per cm2The mass of the laid wheat seeds is 0.13 g-0.26 g.
Further, the distance between the bottom of the wheat water planting seedling tray and the empty tray is 5-15 cm.
Furthermore, the roots of the wheat hydroponic seedling trays are not shoveled (the roots of the wheat seedling trays can be vertically drained or the clean towel is used for sucking out moisture), the roots of the wheat hydroponic seedling trays placed back to back are close to each other, and the mutual moisture preservation effect is achieved.
Further, in the step S3-4, a sand table is paved to be 2-4 cm thick, and the sand is sterilized by high-temperature dry heat of 120 ℃ for more than 2 hours.
Furthermore, the indoor temperature of the feeding frame and the insect cage is 20-30 ℃, and the photoperiod L: D is 12-16 h: 12-8 h. The indoor humidity is not enough, and a humidifier can be placed near the insect cage to improve the humidity of the local space.
Further, in step S4, before using, the insect-raising cage and the glass bottle are scalded with boiled water or steam to kill the pathogens.
Further, the bottom empty tray can be independently placed per unit so as to adapt to different biological breeding schedules of spodoptera frugiperda.
The method for group breeding of spodoptera frugiperda by using the wheat hydroponic seedling disc has the following beneficial effects:
1. the invention utilizes a method for water planting wheat seeds and a seedling tray with fixed size to manufacture the water planting seedling tray with wheat seedlings under indoor conditions. The wheat seedlings can be used as foodstuff to feed spodoptera frugiperda larvae, and can also be used as a spawning trapping medium to attract spodoptera frugiperda adults to spawn, the vertical overlapping structure of the seedling trays also ensures that the wheat seedlings in the feeding period are not collapsed and can maintain the characteristics of fresh and alive growth, and the wheat seedling trays are easy to obtain indoors, so that the annual cultivation of fresh feed can be continued.
2. The invention fully utilizes the structural characteristics that the seedling trays with fixed specifications bear wheat seedlings, utilizes the characteristic that spodoptera frugiperda larvae are favored to eat under the condition of light shading, utilizes the seedling channels in the wheat seedling trays to be arranged and embedded in odd numbers and even numbers, fully utilizes the characteristic that the wheat seedlings on two sides are stiff and have seedling roots, forms a three-dimensional space with wide longitudinal thickness by the wheat seedlings which stand at high density, and can eat from top to bottom, effectively isolates the killing behavior generated by the contact of the spodoptera frugiperda larvae, improves the feeding density, and forms the characteristic that the roots and the stems among the seedling tray combined units are close to each other to keep moisture so as to maintain the fresh and alive growth of the seedlings in the feeding period; meanwhile, sand is provided at the bottom of the wheat seedling tray unit for pupation of mature larvae of Spodoptera frugiperda, so that the pupae can be collected in a centralized manner in the later period, and large-scale breeding is realized.
3. The method gets rid of the limitation of using fresh corn leaves for raising Spodoptera frugiperda, can realize large-scale subculture of Spodoptera frugiperda by alternately combining the process flow of raising wheat seedling trays and the flow of raising Spodoptera frugiperda indoors, is easy to obtain fresh food, and provides a physiological striking insect source for developing scientific researches on biology, ecology, physiology and the like of Spodoptera frugiperda and propagation of natural enemy parasitic wasps in future.
Drawings
FIG. 1 is a schematic view of the structure of the rearing stand (with an empty tray at the bottom) of the present invention;
fig. 2 is a schematic structural diagram of fig. 1 with the sand table replaced by the hollow plate.
In the figure: 1. a feeding rack; 2. a feeding unit; 3. empty disks; 4. a support frame; 5. wheat seedlings; 6. and (4) a sand table.
Detailed Description
Further features and advantages of the present invention will be understood from the following detailed description. The examples provided are merely illustrative of the method of the present invention and do not limit the remainder of the disclosure in any way. The experimental procedures in the following examples are all conventional ones unless otherwise specified. Materials and the like used in the following examples are commercially available unless otherwise specified.
Example 1 (wheat seed)
The invention relates to a method for grouplenization breeding of spodoptera frugiperda by wheat hydroponic seedling disks, which comprises the following steps:
(1) preparing a wheat water culture seedling tray:
a. weighing 100g of wheat seeds, washing, soaking in tap water for 12 hours, dividing into 5 parts, sowing wheat seeds along the seedling channels 1, 3 and 5cm of the seedling tray with a net in a plastic tray with a size of 23 multiplied by 30cm (the spread size of the wheat seeds is about 21 multiplied by 28cm), wherein the sowing width is 5cm, sowing wheat seeds along the seedling channels 2 and 4 of the seedling tray corresponding to the empty seedling channel, and enabling the water level to reach meshes without submerging the seeds, and placing the seedling tray under the water level to be not less than 4cm for cultivation in a room temperature environment;
b. after 9-10 days, when the green wheat seedlings grow to 15-20cm, taking out the wheat seedling tray, draining, preparing a sterilized towel, soaking in water, wringing, spreading on a table top, placing the wheat seedling tray on the towel, and wrapping for later use;
c. as shown in fig. 1, the wheat seedling trays are vertically placed and fixed on a support frame 4, the same odd-numbered seedling trays or even-numbered seedling trays are fixed back to form a feeding unit 2, the adjacent feeding units are odd-even, and the opposite wheat water-cultured seedlings 5 are staggered, mutually embedded and oppositely arranged to form a feeding frame 1 for arranging seedlings and roots; and an empty tray 3 with the length of 30 multiplied by 30cm is placed at the bottom; on the basis of the above-mentioned structure, the seedling trays can be continuously placed in the longitudinal direction.
(2) The breeding process of Spodoptera frugiperda larvae comprises the following steps:
a. respectively putting 200-400 spodoptera frugiperda egg blocks into the three wheat seedling feeding units, feeding the hatched larvae with young and tender wheat seedlings, and dispersedly feeding after the larvae are 2-3 years old;
b. considering that the wheat seedlings are still in a growth state, the wheat seedling tray is replaced and the insect manure and seedling residues are cleaned in the feeding process for 3-7 days, and if the wheat seedlings are not eaten, the wheat seedlings can be pulled out and repeatedly stacked in a fresh seedling tray;
c. when the larvae grow to 4 th instar and the density of the larvae reaches 20 heads/plate, other wheat seedling plates are timely separated, the terminal killing behavior caused by mutual contact of the larvae after 4 th instar is avoided,
d. after the larvae grow to 5-6 years old, when the population density reaches 30 heads/disc, separating the wheat seedling disc in time for treatment, replacing the wheat seedling disc at the bottom, placing a sand disc 6 (shown in figure 2) with the size of 30 multiplied by 30cm, and paving sand with the thickness of 2cm at the bottom for killing by dry heat at high temperature of 120 ℃ for 2h so that the aged larvae enter the sand to pupate;
(3) collecting pupae, feeding imagoes, preserving eggs and subculturing:
a. after the aged larvae pupate in sand, screening out the aged larvae from the sand by using a 10-mesh sample separation screen, putting the aged larvae into a glass bottle with a paper towel at the bottom pad of the glass bottle, tying a nylon net with 100 meshes at the bottle mouth by using a rubber band, and standing at room temperature until pupae eclosion;
b. putting the eclosion adult insects into an insect cage of 40 multiplied by 40cm made of 100-mesh nylon mesh, placing 1 wheat seedling tray with the same size (the seedling trays in odd rows and the seedling trays in even rows are random) at the bottom for the adult insects to lay eggs, and hanging cotton balls dipped with 10% honey water for the adult insects to supplement nutrition;
c. changing cotton balls and collecting egg masses every day, pulling out wheat seedlings with the egg masses, or putting the collected spodoptera frugiperda egg masses into a refrigerator at 5 ℃ for storage for 2 days, taking out the wheat seedlings when the wheat seedlings are used, or directly placing the wheat seedlings in the feeding frame containing the water culture wheat seedlings, and circularly propagating and feeding the wheat seedlings;
d. the relative humidity of the inside of the insect cage is about 80%, and the room temperature is 25-28 ℃, so that the Spodoptera frugiperda larvae can develop and adult insects can lay eggs in the indoor environment.
Example 2
The invention relates to a method for group breeding Spodoptera frugiperda by using wheat hydroponic seedling discs, which comprises the following steps:
(1) preparing a wheat water culture seedling tray:
a. weighing 125g of wheat seeds, washing, soaking for 12 hours by using tap water, dividing into 5 parts, sowing the wheat seeds along the seedling channels 1, 3 and 5cm of the seedling tray with a net in a plastic tray with a size of 23 multiplied by 30cm (the spread size of the wheat seeds is about 21 multiplied by 28cm), wherein the sowing width is 5cm, sowing the wheat seeds along the seedling channels 2 and 4cm of the seedling tray corresponding to the empty seedling channel, enabling the water level to reach meshes without submerging the height of the seeds, and placing the seedling tray under the water level not less than 4cm for cultivation in a room temperature environment;
b. after 9-10 days, when the green wheat seedlings grow to 15-20cm, taking out the two wheat seedling trays, draining, preparing a sterilized towel, soaking in water, wringing, flatly paving on a table top, and placing the wheat seedling trays on the towel and wrapping for later use;
c. as shown in fig. 1, the wheat seedling trays are vertically arranged and fixed on a support frame 4, the same odd seedling trays or even seedling trays are fixed back to form breeding units 2, the adjacent breeding units are odd-even, and the opposite wheat water culture seedlings 5 are staggered, mutually embedded and oppositely arranged to form a breeding frame 1 for arranging seedlings and roots; and an empty tray 3 of 30 x 30cm is placed at the bottom;
(2) the breeding process of Spodoptera frugiperda larvae comprises the following steps:
a. respectively putting 360-plus 500 spodoptera frugiperda egg blocks into the three wheat seedling feeding units, feeding the hatched larvae with young and tender wheat seedlings, and dispersedly feeding after the larvae are 2-3 years old;
b. considering that the wheat seedlings are still in a growth state, the wheat seedling tray is replaced and insect manure and seedling residues are cleaned in the feeding process for 3-7 days, and if the wheat seedlings are not eaten, the wheat seedlings can be pulled out and repeatedly stacked on the wheat seedling tray unit;
c. when the density of the larvae reaches 20 heads/feeding unit after the larvae grow to 4 years old, timely separating other wheat seedling feeding units to avoid the killing action caused by mutual contact of the larvae after 4 years old,
d. after the larvae grow to 5-6 years old, when the density of the larvae reaches 30 heads/disc, separating the wheat seedling disc in time, replacing the wheat seedling disc at the bottom, placing a sand table 6 (shown in figure 2) with the size of 30 multiplied by 30cm, and paving sand with the thickness of 2cm which is killed by dry heat at high temperature of 120 ℃ for 2h at the bottom so that the aged larvae enter the sand to be pupated;
(3) collecting pupae, feeding imagoes, preserving eggs and subculturing:
a. after the aged larvae pupate in sand, screening out the aged larvae from the sand by using a 10-mesh sample separation screen, putting the aged larvae into a glass bottle with a paper towel at the bottom pad of the glass bottle, tying a nylon net with 100 meshes at the bottle mouth by using a rubber band, and standing at room temperature until pupae eclosion;
b. putting the eclosion imagoes into a 40 multiplied by 40cm insect cage made of 100-mesh nylon mesh cloth, placing 1 wheat seedling tray (random for odd seedling trays and even seedling trays) at the bottom for spawning, and hanging cotton balls dipped with 10% honey water for supplementing nutrition;
c. changing cotton balls and collecting egg masses every day, pulling out wheat seedlings with the egg masses, or storing the collected spodoptera frugiperda egg masses in a refrigerator at 5 ℃ for 2 days, taking out the egg masses when the egg masses are used, or directly placing the egg masses in a rearing cage containing the water-cultured wheat seedlings, and circularly propagating and rearing;
d. the indoor relative humidity of the insect cage is about 80%, and the room temperature is 25-28 ℃, so that the development of spodoptera frugiperda larvae and the oviposition of adults are facilitated in the indoor environment.
Example 3
The invention relates to a method for group breeding Spodoptera frugiperda by using wheat hydroponic seedling discs, which comprises the following steps:
(1) preparing a wheat water culture seedling feeding unit:
a. weighing 85g of wheat seeds, washing, soaking in tap water for 12 hours, dividing into 5 parts, sowing wheat seeds along the seedling channels 1, 3 and 5cm of the seedling tray with a net in a plastic tray with a size of 23 multiplied by 30cm (the spread size of the wheat seeds is about 21 multiplied by 28cm), wherein the sowing width is 5cm, sowing wheat seeds along the seedling channels 2 and 4cm of the seedling tray corresponding to the empty seedling channel, and allowing the water level to reach meshes without submerging the seeds, and placing the seedling tray under the water level not less than 4cm for cultivation in a room temperature environment;
b. after 9-10 days, when the green wheat seedlings grow to 15-20cm, taking out the two wheat seedling trays, draining, preparing a sterilized towel, soaking in water, wringing, flatly paving on a table top, and placing the wheat seedling trays on the towel and wrapping for later use;
c. as shown in fig. 1, the wheat seedling trays are vertically placed and fixed on a support frame 4, the same odd-numbered seedling trays or even-numbered seedling trays are fixed back to form a feeding unit 2, the adjacent feeding units are odd-even, and the opposite wheat water-cultured seedlings 5 are staggered, mutually embedded and oppositely arranged to form a feeding frame 1 for arranging seedlings and roots; and an empty tray 3 of 30 x 30cm is placed at the bottom;
(2) the breeding process of Spodoptera frugiperda larvae comprises the following steps:
a. respectively feeding the hatched larvae with 350-550 spodoptera frugiperda eggs to the three wheat seedling feeding units, and dispersedly feeding the hatched larvae with young wheat seedlings after the larvae are 2-3 years old;
b. considering that the wheat seedlings are still in a growth state, the wheat seedling tray is replaced and insect manure and seedling residues are cleaned in the feeding process for 3-7 days, and if the wheat seedlings are not eaten, the wheat seedlings can be pulled out and repeatedly stacked on the wheat seedling tray unit;
c. when the density of the larvae reaches 20 heads/feeding unit after the larvae grow to 4 years old, timely separating other wheat seedling feeding units to avoid the killing action caused by mutual contact of the larvae after 4 years old,
d. after the larvae grow to 5-6 years old, when the population density reaches 30 heads/disc, separating the wheat seedling disc in time for treatment, replacing the wheat seedling disc at the bottom, placing a sand disc 6 with the size of 30 multiplied by 30cm, and paving sand with the thickness of 2cm which is killed by dry heat at high temperature of 120 ℃ for 2h at the bottom of the sand disc so that the aged larvae enter the sand to pupate;
(3) collecting pupae, feeding imagoes, preserving eggs and subculturing:
a. after the aged larvae pupate in sand, screening out the aged larvae from the sand by using a 10-mesh sample separation screen, putting the aged larvae into a glass bottle with a paper towel at the bottom pad of the glass bottle, tying a nylon net with 100 meshes at the bottle mouth by using a rubber band, and standing at room temperature until pupae eclosion;
b. putting the eclosion adult insects into an insect cage of 40 multiplied by 40cm made of 100-mesh nylon mesh, placing 1 wheat seedling tray (random in odd-numbered seedling trays and even-numbered seedling trays) at the bottom for the adult insects to lay eggs, and hanging cotton balls dipped with 10% honey water for the adult insects to supplement nutrition;
c. changing cotton balls and collecting egg masses every day, pulling out wheat seedlings with the egg masses, or putting the collected spodoptera frugiperda egg masses into a refrigerator at 5 ℃ for storage for 2 days, taking out the wheat seedlings when the wheat seedlings are used, or directly putting the wheat seedlings into the rearing cage containing the water culture wheat seedlings, and circularly propagating and rearing;
d. the indoor relative humidity of the insect cage is about 80%, and the room temperature is 25-28 ℃, so that the development of spodoptera frugiperda larvae and the oviposition of adults are facilitated in the indoor environment.
Control group
Fresh corn leaves are mainly used as spodoptera frugiperda larva food and as spodoptera frugiperda imago egg laying medium, and the method comprises the following steps:
(1) the breeding process of Spodoptera frugiperda larvae comprises the following steps:
a. respectively flatly paving the corn leaves in plastic trays with nets, wherein the size of each tray is about 23 multiplied by 30cm, stacking new staggered and stacked corn leaves with different sizes, putting 400 spodoptera frugiperda egg blocks containing 200 eggs, and taking the young tender corn leaves from the hatched larvae until the larvae are 2-3 years old and then taking the young corn leaves in a dispersing way;
b. replacing corn leaves every 3 days in the feeding process and cleaning insect manure and seedling residues;
c. after the larvae grow to 4 th instar and the density of the larval mouths reaches 20 heads/disc, other corn leaf discs are timely separated to avoid the terminal killing action caused by mutual contact of the larvae after 4 th instar,
d. after the larvae grow to 5-6 years old, when the density of the larvae reaches 30 heads/plate, separating corn leaf plates in time, placing a sand plate with the size of 30 multiplied by 30cm at the bottom of the rearing cage, and laying sand with the thickness of 2cm on the bottom of the rearing cage, and sterilizing the sand for 2 hours by dry heat at high temperature of 120 ℃. The bottoms of the corn-containing plates on the two sides are leaned against a sand table, and a distance of 3-5cm is reserved so that aged larvae can enter sand to pupate;
e. 3 treatments were set simultaneously as replicates.
(3) Collecting pupae, feeding imagoes, preserving eggs and subculturing:
a. after the aged larvae pupate in sand, screening out the aged larvae from the sand by using a 10-mesh sample separation screen, putting the aged larvae into a glass bottle with a paper towel at the bottom pad of the glass bottle, tying a nylon net with 100 meshes at the bottle mouth by using a rubber band, and standing at room temperature until pupae eclosion;
b. putting the eclosion imagoes into a 40 multiplied by 40cm insect cage made of 100-mesh nylon mesh cloth, hanging corn leaves at the top for inducing the imagoes to lay eggs, and hanging cotton balls dipped with 10% honey water for supplementing nutrition;
c. replacing cotton balls and corn leaves every day, directly placing the cotton balls and the corn leaves in the rearing cage containing the corn leaves, and circularly propagating and rearing;
d. the indoor relative humidity of the insect cage is about 80%, and the room temperature is 25-28 ℃, so that the development of spodoptera frugiperda larvae and the oviposition of adults are facilitated in the indoor environment.
Comparative results
Each pupae was weighed and recorded on the first day of pupation using a one-ten-thousandth electronic balance.
Calculated using the following formula:
the hatching rate (number of newly hatched larvae/total number of eggs tested) × 100%
The survival rate of the larva is (the number of the aged larva/the total number of the tested larva) multiplied by 100 percent
Pupation rate (pupation number/number of tested larvae) x 100%
The eclosion rate is (eclosion insect number/pupa number) multiplied by 100 percent
TABLE 1 comparison of biological parameters of Spodoptera frugiperda with different wheat hydroponic seedling tray feeding modes
| Feeding mode | Survival rate of larvae/%) | Pupation Rate/% | Weight of pupa/mg | Eclosion Rate/% | Egg hatchability/% |
| Example 1 | 60.29±4.72a | 92.21±3.65a | 211.1±9.4a | 84.67±3.75a | 98.34±0.45a |
| Example 2 | 58.94±5.13a | 90.27±2.45a | 205.2±7.5a | 85.45±2.54a | 96.1±0.50a |
| Example 3 | 65.14±3.15a | 93.56±2.1a | 231.2±8.6a | 87.2±3.82a | 95.14±0.43a |
| |
30.23±5.96b | 73.1±6.25b | 193.2±6.7a | 80.25±4.12a | 94.65±0.39a |
As can be seen from table 1: the method of the present invention (examples 1-3) was used, namely, a method of feeding spodoptera frugiperda larvae in a herdized manner using a wheat water-cultured seedling unit to feed spodoptera frugiperda larvae, and using the larvae as egg-laying trapping medium to attract the imagoes of spodoptera frugiperda to lay eggs, wherein the survival rates of the larvae were 60.29%, 58.94% and 65.14%, respectively; pupation rates of 92.21%, 90.27% and 93.56% respectively; the survival rate of the larvae and the pupation rate of the larvae are respectively 30.23 percent and 73.1 percent which are obviously higher than those of the larvae bred by the corn leaves in the control group 1, and particularly, the survival rate of the larvae is far lower than that of the larvae in the examples 1 to 3 of the invention. In contrast, the hatchability of the eggs in examples 1 to 3 of the present invention was 98.34%, 96.1% and 95.14%, the pupa weight was 211.1mg, 205.2mg and 231.2mg, and the eclosion rate was 84.67%, 85.45% and 87.2%, respectively, which were higher than the results of the control group 1 fed with corn leaves. These results have shown that utilize wheat water planting seedling tray unit to feed Spodoptera frugiperda larva, and regard as the medium of laying eggs of luring Spodoptera frugiperda imago with wheat water planting seedling tray unit, the contrast uses the mode of raising of maize leaf, higher larva survival rate and the rate of pupating have, show that can effectively reduce each other of larva and kill by the crisscross structure of crisscross wheat seedling tray, show the survival rate that improves Spodoptera frugiperda larva, and with the mode that uses sand tray to collect the pupa in the bottom, and improved the rate of pupating of pupa, the egg hatching rate that has also obtained equal effect simultaneously, pupa weight and the rate of eclosion. The invention shows that the Spodoptera frugiperda is bred in a group by utilizing the wheat water culture seedling disc units, and provides a large number of uniformly-developed insect sources for test for the application of control technologies such as developing the biological ecology of the Spodoptera frugiperda, breeding natural enemy parasitic wasps and the like.
The above disclosure is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the scope of the present invention, therefore, the present invention is not limited by the appended claims.
Claims (9)
1. A method for grouplily feeding Spodoptera frugiperda by utilizing wheat hydroponic seedling discs is characterized by comprising the following steps:
step S1, preparing a wheat water culture seedling tray:
taking wheat seeds, washing, soaking in clear water, sowing the wheat seeds in odd seedling channels of a first seedling tray, sowing the wheat seeds in even seedling channels of a second seedling tray, obtaining odd seedling trays and even seedling trays, suspending the seedling trays with holes on the water surface, standing for 9-12 days, taking out the seedling trays, and draining water to obtain a wheat water-culture seedling tray;
step S2, constructing a feeding rack:
the feeding frame comprises a bottom empty tray which is horizontally placed and a plurality of wheat water-cultured seedling trays which are vertically placed above the bottom empty tray, the same odd-numbered seedling trays or even-numbered seedling trays are fixed back to form feeding units, the adjacent feeding units are odd-even, so that the opposite wheat water-cultured seedlings are staggered, mutually embedded and oppositely arranged, and the feeding frame with seedling-to-seedling and root-to-root arrangement is formed;
step S3, feeding Spodoptera frugiperda larvae:
step S3-1: quantitatively adding spodoptera frugiperda egg blocks at the top of the wheat water culture seedling tray, wherein the ratio of the number of the eggs to the area density of the wheat seedlings is 1: 0.59-1.7 cm2The hatched larvae eat young wheat seedlings, the larvae automatically disperse and eat after 2-3 years, and the residual seedlings gradually fall into a lower wheat seedling path;
step S3-2: replacing the wheat water culture seedling tray for 3-7 days in the feeding process, cleaning insect manure and broken seedling residues, repeatedly stacking the wheat water culture seedling tray after pulling out fresh wheat seedlings which are not eaten on the wheat water culture seedling tray, putting the larvae falling into an empty tray on the wheat seedlings at the top of the wheat water culture seedling tray, and continuously feeding the larvae until the larvae are mature;
step S3-3: after the larvae grow to 4 years old, the population density reaches 1 head to 20cm2When the larvae grow to 5-6 years old, the density of the larvae reaches 1 head at 30cm2In time, the larvae should be separated in time,the killing action caused by mutual contact of larvae after 4 years old is avoided;
step S3-4: replacing the bottom empty tray with a sand tray containing sand after the larvae grow to 5-6 years old so that the aged larvae enter the sand to pupate;
step S4, collection of pupae, adult breeding, preservation of eggs and subculture:
s4-1, after pupating the larva to be aged in sand, screening out the larva from the sand, putting the larva into a clean glass bottle, and standing until the pupa eclosion;
s4-2, putting eclosion adults into an insect cage, placing a wheat seedling tray at the bottom of the insect cage for inducing the adults to lay eggs, and hanging cotton balls dipped with honey water for supplementing nutrition;
and S4-3, replacing cotton balls and collecting egg blocks every day, pulling out wheat seedlings with the egg blocks, or putting the collected spodoptera frugiperda egg blocks into a refrigerator at 5 ℃ for storage for 2 days, taking out the wheat seedlings when the wheat seedlings are used, or directly placing the wheat seedlings in the wheat water culture seedling tray of the step S3, and circularly propagating and breeding the wheat seedlings.
2. The method for mass rearing of spodoptera frugiperda using wheat hydroponic seedling trays according to claim 1, wherein: in the step S1, the wheat seeds are soaked in clear water for 8-14 h.
3. The method for mass rearing of spodoptera frugiperda using wheat hydroponic seedling trays according to claim 1, wherein: in the step S1, the sowing width of the wheat seeds is 4-10 cm.
4. The method for mass rearing of spodoptera frugiperda using wheat hydroponic seedling trays according to claim 1, wherein: in step S1, seedling tray is arranged per cm2The mass of the laid wheat seeds is 0.13 g-0.26 g.
5. The method for mass rearing of spodoptera frugiperda using wheat hydroponic seedling trays according to claim 1, wherein: the distance between the bottom of the wheat water planting seedling tray and the empty tray is 5-15 cm.
6. The method for mass rearing of spodoptera frugiperda using wheat hydroponic seedling trays according to claim 1, wherein: the root of the bottom of the wheat water planting seedling tray is not eradicated, and the root of the wheat water planting seedling tray placed back to back is close to each other.
7. The method for groupwise feeding spodoptera frugiperda using hydroponic seedling disks of wheat as claimed in claim 1, wherein: in the step S3-4, the sand table is laid to be 2-4 cm thick, and the sand is sterilized by high-temperature 120 ℃ dry heat for more than 2 hours.
8. The method for mass rearing of spodoptera frugiperda using wheat hydroponic seedling trays according to claim 1, wherein: the indoor temperature of the feeding frame and the insect cage is 20-30 ℃, and the photoperiod L: D is 12-16 h: 12-8 h.
9. The method for groupwise feeding spodoptera frugiperda using hydroponic seedling disks of wheat as claimed in claim 8, wherein: in step S4, the insect-raising cage and the glass bottle are used and then are scalded by boiled water or steam to kill the pathogens.
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| CN210987775U (en) * | 2019-09-02 | 2020-07-14 | 代彤 | Indoor quick artificial containers of spodoptera frugiperda |
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| CN105230382A (en) * | 2015-09-16 | 2016-01-13 | 天津市宝宏塑胶制品有限公司 | Nursery seedling plate |
| CN210987775U (en) * | 2019-09-02 | 2020-07-14 | 代彤 | Indoor quick artificial containers of spodoptera frugiperda |
| CN111758672A (en) * | 2020-07-16 | 2020-10-13 | 中国热带农业科学院环境与植物保护研究所 | Group breeding method for feeding spodoptera frugiperda larvae with young corn shoots |
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