CN109156437A - A kind of method of In Vitro Rearing of Trichogramma low-temperature storage - Google Patents
A kind of method of In Vitro Rearing of Trichogramma low-temperature storage Download PDFInfo
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- CN109156437A CN109156437A CN201810866607.6A CN201810866607A CN109156437A CN 109156437 A CN109156437 A CN 109156437A CN 201810866607 A CN201810866607 A CN 201810866607A CN 109156437 A CN109156437 A CN 109156437A
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- trichogramma
- storage
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- temperature
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- 238000003860 storage Methods 0.000 title claims abstract description 41
- 241000256618 Trichogramma Species 0.000 title claims abstract description 21
- 238000000034 method Methods 0.000 title claims abstract description 13
- 238000000338 in vitro Methods 0.000 title claims abstract description 10
- 230000000384 rearing effect Effects 0.000 title claims abstract description 10
- 235000013601 eggs Nutrition 0.000 claims abstract description 18
- 239000007788 liquid Substances 0.000 claims abstract description 7
- 230000000694 effects Effects 0.000 abstract description 6
- 230000032669 eclosion Effects 0.000 description 15
- 241000382353 Pupa Species 0.000 description 9
- 241000186214 Trichogramma dendrolimi Species 0.000 description 9
- 238000011161 development Methods 0.000 description 7
- 230000018109 developmental process Effects 0.000 description 7
- 230000001418 larval effect Effects 0.000 description 6
- 244000045947 parasite Species 0.000 description 6
- 238000000692 Student's t-test Methods 0.000 description 4
- 230000001488 breeding effect Effects 0.000 description 4
- 230000017448 oviposition Effects 0.000 description 4
- 238000012545 processing Methods 0.000 description 4
- 238000012353 t test Methods 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 102000002322 Egg Proteins Human genes 0.000 description 3
- 108010000912 Egg Proteins Proteins 0.000 description 3
- 230000007423 decrease Effects 0.000 description 3
- 210000004681 ovum Anatomy 0.000 description 3
- 230000003071 parasitic effect Effects 0.000 description 3
- 241000255789 Bombyx mori Species 0.000 description 2
- 239000000090 biomarker Substances 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 238000012790 confirmation Methods 0.000 description 2
- 238000013401 experimental design Methods 0.000 description 2
- 230000035558 fertility Effects 0.000 description 2
- 230000019617 pupation Effects 0.000 description 2
- 238000013441 quality evaluation Methods 0.000 description 2
- 238000005057 refrigeration Methods 0.000 description 2
- 230000001850 reproductive effect Effects 0.000 description 2
- 230000004044 response Effects 0.000 description 2
- 241000208340 Araliaceae Species 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 238000000585 Mann–Whitney U test Methods 0.000 description 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 1
- 235000003140 Panax quinquefolius Nutrition 0.000 description 1
- 241001414987 Strepsiptera Species 0.000 description 1
- 210000001136 chorion Anatomy 0.000 description 1
- 210000002969 egg yolk Anatomy 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 235000008434 ginseng Nutrition 0.000 description 1
- 230000000266 injurious effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses a kind of methods of In Vitro Rearing of Trichogramma low-temperature storage.It is that the trichogramma for cultivating the artificial eggs liquid of prepupal period stores.Method of the invention as far as possible extension storage period, can be easy to use on the basis of not influencing numerous bee quality and application effect.
Description
Technical field
The invention belongs to field of biological control, and in particular to a kind of method of In Vitro Rearing of Trichogramma low-temperature storage.
Background technique:
Trichogramma (Trichogramma) belongs to a kind of parasitic insect of Hymenoptera Trichogramma spp.Adult worms producting eggs are in host
In ovum, larval feeding yolk is pupated, and causes host dead.Host's chorion of breaking by the teeth after adult eclosion is gone out free living.Adult
Body is 0.3-1.0 millimeters long, is a kind of highly advantageous natural enemy with value, is widely used in the prevention and treatment of agriculture and forestry injurious insect.However for side
Just it uses, there is demand to be able to extend its storage period as far as possible.
Summary of the invention:
The object of the present invention is to provide a kind of methods of In Vitro Rearing of Trichogramma low-temperature storage, can be not using this method
On the basis of influencing numerous bee quality and application effect, extend storage period as far as possible.
The method of In Vitro Rearing of Trichogramma low-temperature storage of the invention, which is characterized in that be by the artificial eggs liquid of prepupal period
The trichogramma of cultivation is stored.
It further preferably, is that the trichogramma for cultivating the artificial eggs liquid of prepupal period stores in 13 DEG C.Longest Storage period
It is 4 weeks, does not influence numerous bee quality and application effect.
Therefore, method of the invention can extend as far as possible storage on the basis of not influencing numerous bee quality and application effect
Phase is easy to use.
Specific embodiment:
The trichogramma dendrolimi of Fig. 1 extracorporeal culture in different growing periods at different temperatures it is cold deposit different time after plumage
Rate, * is indicated to examine through Dunnett ' S t-test and is compareed (27 DEG C) significant difference (P≤0.05) on column；
The trichogramma dendrolimi of Fig. 2 extracorporeal culture is deposited after different time just in different growing periods are cold at different temperatures
Normal bee amount, * is indicated to examine through Dunnett ' S t-test and is compareed (27 DEG C) significant difference (P≤0.05) on column；
The trichogramma dendrolimi of Fig. 3 extracorporeal culture deposits different time stepmother's generation in different growing periods are cold at different temperatures
With the property ratio of filial generation, * is indicated to examine through Dunnett ' S t-test and is compareed (27 DEG C) significant difference (P≤0.05) on column；
The trichogramma dendrolimi of Fig. 4 extracorporeal culture in different growing periods at different temperatures it is cold deposit it is numerous after different time
Power is grown, * is indicated to examine through Dunnett ' S t-test and compareed (27 DEG C) significant difference (P≤0.05) on column；
The trichogramma dendrolimi of Fig. 5 extracorporeal culture in different growing periods at different temperatures it is cold deposit different time after production
The ovum phase, * is indicated to examine through Mann-Whitney U test and is compareed (27 DEG C) significant difference (P≤0.05) on column.
Specific embodiment:
The following examples are further illustrations of the invention, rather than limiting the invention.
Embodiment 1:
It is pair with the trichogramma dendrolimi (Trichogramma dendrolimi) that artificial eggs liquid cultivates (extracorporeal culture)
As studying influence of the low temperature stress to its growth and development.On the basis of not influencing numerous bee quality and application effect, determine artificial
Most suitable storage temperature, worm state and the Storage period of egg parasite.
Three factors are arranged in this test, and 3 repetitions of every processing are respectively:
A.
Storage worm state: the duration that this test is developed under conditions of 27 DEG C of optimum temperature and relative humidity 75% with trichogramma
Worm state is controlled, is 48h, 96h, 144h and 192h from first oviposition to adult eclosion elapsed-time standards.The confirmation development worm under anatomical lens
State.
B.
Storage temperature: experimental design (4 ± 1) DEG C, (7 ± 1) DEG C, (10 ± 1) DEG C and (13 ± 1) DEG C 4 temperature.
C. storage time: with one week (7d) for a stage, in totally 4 stage, surrounding 28d is lasted.
Biological indicator and quality evaluation parameter:
From parasitic rate, percentage of pupation, eclosion rate, normal bee rate, female and male ratio, Life of Adult, generation time, reproductive capacity and its
Trend index of population etc. investigates response of the In Vitro Rearing of Trichogramma to low temperature stress.
Using life-table method, determines most suitable storage temperature, worm state and the Storage period of artificial egg parasite and investigate artificial egg parasite
The potential of parasitic natural host ovum.
As a result as shown in Fig. 1,2,3,4,5, the results showed that three factors and its reciprocation all significantly affect each biology ginseng
Number, wherein the influence with Storage period and temperature to development and breeding is maximum.Compared with the control, the queen bee ratio base in female generation and filial generation
This is not influenced by cold deposit.Cold to deposit more than two weeks, eclosion rate will receive influence, to influence other biological parameters.
It is deposited 1-3 weeks and prepupal period (96h) is cold at 13 DEG C deposits 1-4 weeks in addition to larval phase (48h) is cold at 10 DEG C, eclosion rate is all with storage
It deposits the extension of phase and declines.Larval phase is cold at 4 DEG C and 7 DEG C to deposit, and eclosion rate, fecundity and egg-laying season all sharply decline and contract
It is short, even without at bee eclosion.4 DEG C and 10 DEG C prepupal period is influenced it is maximum, but can be obtained at 13 DEG C with compare it is similar
Breeding effect.Pupa time is seriously affected by low-temperature storage, can only be no more than 2 weeks in 13 DEG C of refrigerations.Although pupa advanced stage is in difference
There is into bee eclosion after temperature storage, but it is significant with contrast difference.It screens 7 processing and compares constructed life table, it is clear
Best storage worm state is prepupal period, and optimum refrigerated storage temperature is 13 DEG C, and longest Storage period is 4 weeks.
Embodiment 2:
Artificial eggs, egg of oak silkworm cultivate the low-temperature storage experimental method of trichogramma
With the trichogramma dendrolimi (Trichogramma dendrolimi) of artificial eggs liquid and egg of oak silkworm cultivation for object,
Study influence of the low temperature stress to its growth and development.On the basis of not influencing numerous bee quality and application effect, artificial eggs are determined
Most suitable storage temperature, worm state and the Storage period of bee and tussah egg parasite.
Three factors are arranged in this test, and 3 repetitions of every processing are respectively:
A. store worm state: this test with trichogramma 27 DEG C of optimum temperature with developed under conditions of relative humidity 75% when
Long control worm state, from first oviposition to adult eclosion elapsed-time standards be 48h (small grub phase), 96h (prepupal period), 144h (pupa time) and
192h (pupa advanced stage).The confirmation development worm state under anatomical lens.
B. storage temperature: experimental design (4 ± 1) DEG C, (7 ± 1) DEG C, (10 ± 1) DEG C and (13 ± 1) DEG C 4 temperature.
C. storage time: with one week (7d) for a stage, in totally 4 stage, surrounding 28d is lasted.
Biological indicator and quality evaluation parameter:
From parasitic rate, percentage of pupation, eclosion rate, normal bee rate, female and male ratio, Life of Adult, generation time, reproductive capacity and its
Trend index of population etc. investigates response of the In Vitro Rearing of Trichogramma to low temperature stress.
Artificial egg parasite experimental result (table 1)
The result shows that (Fig. 1-Fig. 5) three factors and its reciprocation all significantly affect each biological parameter, wherein with storage
The influence of phase and temperature to development and breeding is maximum.Compared with the control, female queen bee ratio for filial generation is not substantially by cold
The influence deposited.Cold to deposit more than two weeks, eclosion rate will receive influence, to influence other biological parameters.In addition to larval phase
(48h) is cold at 10 DEG C to be deposited 1-3 weeks and prepupal period (96h) is cold at 13 DEG C deposits 1-4 weeks, and eclosion rate is all with the extension of Storage period
And decline.Larval phase is cold at 4 DEG C and 7 DEG C to deposit, and eclosion rate, fecundity and egg-laying season all sharply decline and shorten, even without
At bee eclosion.4 DEG C and 10 DEG C influence maximum to prepupal period, but can obtain at 13 DEG C and compare similar breeding effect.Pupa
Phase is seriously affected by low-temperature storage, can only be no more than 2 weeks in 13 DEG C of refrigerations.Although pupa advanced stage different temperatures storage after all
There is into bee eclosion, but it is significant with contrast difference.It screens 7 processing and compares constructed life table, specify best storage worm
State is prepupal period, and optimum refrigerated storage temperature is 13 DEG C, and longest Storage period is 4 weeks.
Tussah egg parasite experimental result (table 1)
Compared with the control, female generation and the queen bee ratio of filial generation are not influenced by cold deposit substantially.It is cold to deposit more than two weeks, plumage
Rate will receive influence, to influence other biological parameters.The Optimum storage phase is can to store at 4 DEG C in larval phase or pupa time
4 weeks, pupa time can store 4 weeks at 7 DEG C.It has been more than threshold of development under the conditions of 13 DEG C, each developmental stage stores up at 13 DEG C
The bee out that sprouted wings in incubator more than 3-4 weeks is deposited, the temperature as low-temperature storage is not suitable for.Prepupal period is at 7 DEG C, pupa time
It cannot all be stored more than 2 weeks at 10 DEG C.
Table 1
Claims (2)
1. a kind of method of In Vitro Rearing of Trichogramma low-temperature storage, which is characterized in that be to cultivate the artificial eggs liquid of prepupal period
Trichogramma is stored.
2. the method for In Vitro Rearing of Trichogramma low-temperature storage according to claim 1, which is characterized in that be by prepupal period
The trichogramma that artificial eggs liquid is cultivated is stored in 13 DEG C.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810866607.6A CN109156437A (en) | 2018-08-01 | 2018-08-01 | A kind of method of In Vitro Rearing of Trichogramma low-temperature storage |
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| CN201810866607.6A CN109156437A (en) | 2018-08-01 | 2018-08-01 | A kind of method of In Vitro Rearing of Trichogramma low-temperature storage |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116171941A (en) * | 2023-03-08 | 2023-05-30 | 福建省农业科学院水稻研究所 | Application of wheat seedlings in cold storage of trichogramma longsynanthassemblies and cold storage method |
| CN116235825A (en) * | 2023-04-14 | 2023-06-09 | 广东省科学院动物研究所 | Method for prolonging shelf life of egg parasitic wasps |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104255660A (en) * | 2014-09-01 | 2015-01-07 | 吉林农业大学 | Method for alternately storing trichogramma at intermediate and low temperature in two steps |
| CN108056070A (en) * | 2018-01-16 | 2018-05-22 | 吉林农业大学 | Utilize the method for white snow moths attracted by lamplight breeding trichogramma |
-
2018
- 2018-08-01 CN CN201810866607.6A patent/CN109156437A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104255660A (en) * | 2014-09-01 | 2015-01-07 | 吉林农业大学 | Method for alternately storing trichogramma at intermediate and low temperature in two steps |
| CN108056070A (en) * | 2018-01-16 | 2018-05-22 | 吉林农业大学 | Utilize the method for white snow moths attracted by lamplight breeding trichogramma |
Non-Patent Citations (1)
| Title |
|---|
| 马春森: ""Effects of constant temperature, exposure period, and age on diapause induction in Trichogramma dendrolimi"", 《BIOLOGICAL CONTROL》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116171941A (en) * | 2023-03-08 | 2023-05-30 | 福建省农业科学院水稻研究所 | Application of wheat seedlings in cold storage of trichogramma longsynanthassemblies and cold storage method |
| CN116235825A (en) * | 2023-04-14 | 2023-06-09 | 广东省科学院动物研究所 | Method for prolonging shelf life of egg parasitic wasps |
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Application publication date: 20190108 |