CN109156437A - A kind of method of In Vitro Rearing of Trichogramma low-temperature storage - Google Patents

A kind of method of In Vitro Rearing of Trichogramma low-temperature storage Download PDF

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Publication number
CN109156437A
CN109156437A CN201810866607.6A CN201810866607A CN109156437A CN 109156437 A CN109156437 A CN 109156437A CN 201810866607 A CN201810866607 A CN 201810866607A CN 109156437 A CN109156437 A CN 109156437A
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China
Prior art keywords
trichogramma
storage
low
temperature
period
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CN201810866607.6A
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Chinese (zh)
Inventor
吕欣
李军
韩诗畴
李志刚
李丽英
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Guangdong Institute of Applied Biological Resources
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Guangdong Institute of Applied Biological Resources
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Priority to CN201810866607.6A priority Critical patent/CN109156437A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/033Rearing or breeding invertebrates; New breeds of invertebrates

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Zoology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

The invention discloses a kind of methods of In Vitro Rearing of Trichogramma low-temperature storage.It is that the trichogramma for cultivating the artificial eggs liquid of prepupal period stores.Method of the invention as far as possible extension storage period, can be easy to use on the basis of not influencing numerous bee quality and application effect.

Description

A kind of method of In Vitro Rearing of Trichogramma low-temperature storage
Technical field
The invention belongs to field of biological control, and in particular to a kind of method of In Vitro Rearing of Trichogramma low-temperature storage.
Background technique:
Trichogramma (Trichogramma) belongs to a kind of parasitic insect of Hymenoptera Trichogramma spp.Adult worms producting eggs are in host In ovum, larval feeding yolk is pupated, and causes host dead.Host's chorion of breaking by the teeth after adult eclosion is gone out free living.Adult Body is 0.3-1.0 millimeters long, is a kind of highly advantageous natural enemy with value, is widely used in the prevention and treatment of agriculture and forestry injurious insect.However for side Just it uses, there is demand to be able to extend its storage period as far as possible.
Summary of the invention:
The object of the present invention is to provide a kind of methods of In Vitro Rearing of Trichogramma low-temperature storage, can be not using this method On the basis of influencing numerous bee quality and application effect, extend storage period as far as possible.
The method of In Vitro Rearing of Trichogramma low-temperature storage of the invention, which is characterized in that be by the artificial eggs liquid of prepupal period The trichogramma of cultivation is stored.
It further preferably, is that the trichogramma for cultivating the artificial eggs liquid of prepupal period stores in 13 DEG C.Longest Storage period It is 4 weeks, does not influence numerous bee quality and application effect.
Therefore, method of the invention can extend as far as possible storage on the basis of not influencing numerous bee quality and application effect Phase is easy to use.
Specific embodiment:
The trichogramma dendrolimi of Fig. 1 extracorporeal culture in different growing periods at different temperatures it is cold deposit different time after plumage Rate, * is indicated to examine through Dunnett ' S t-test and is compareed (27 DEG C) significant difference (P≤0.05) on column;
The trichogramma dendrolimi of Fig. 2 extracorporeal culture is deposited after different time just in different growing periods are cold at different temperatures Normal bee amount, * is indicated to examine through Dunnett ' S t-test and is compareed (27 DEG C) significant difference (P≤0.05) on column;
The trichogramma dendrolimi of Fig. 3 extracorporeal culture deposits different time stepmother's generation in different growing periods are cold at different temperatures With the property ratio of filial generation, * is indicated to examine through Dunnett ' S t-test and is compareed (27 DEG C) significant difference (P≤0.05) on column;
The trichogramma dendrolimi of Fig. 4 extracorporeal culture in different growing periods at different temperatures it is cold deposit it is numerous after different time Power is grown, * is indicated to examine through Dunnett ' S t-test and compareed (27 DEG C) significant difference (P≤0.05) on column;
The trichogramma dendrolimi of Fig. 5 extracorporeal culture in different growing periods at different temperatures it is cold deposit different time after production The ovum phase, * is indicated to examine through Mann-Whitney U test and is compareed (27 DEG C) significant difference (P≤0.05) on column.
Specific embodiment:
The following examples are further illustrations of the invention, rather than limiting the invention.
Embodiment 1:
It is pair with the trichogramma dendrolimi (Trichogramma dendrolimi) that artificial eggs liquid cultivates (extracorporeal culture) As studying influence of the low temperature stress to its growth and development.On the basis of not influencing numerous bee quality and application effect, determine artificial Most suitable storage temperature, worm state and the Storage period of egg parasite.
Three factors are arranged in this test, and 3 repetitions of every processing are respectively:
A.
Storage worm state: the duration that this test is developed under conditions of 27 DEG C of optimum temperature and relative humidity 75% with trichogramma Worm state is controlled, is 48h, 96h, 144h and 192h from first oviposition to adult eclosion elapsed-time standards.The confirmation development worm under anatomical lens State.
B.
Storage temperature: experimental design (4 ± 1) DEG C, (7 ± 1) DEG C, (10 ± 1) DEG C and (13 ± 1) DEG C 4 temperature.
C. storage time: with one week (7d) for a stage, in totally 4 stage, surrounding 28d is lasted.
Biological indicator and quality evaluation parameter:
From parasitic rate, percentage of pupation, eclosion rate, normal bee rate, female and male ratio, Life of Adult, generation time, reproductive capacity and its Trend index of population etc. investigates response of the In Vitro Rearing of Trichogramma to low temperature stress.
Using life-table method, determines most suitable storage temperature, worm state and the Storage period of artificial egg parasite and investigate artificial egg parasite The potential of parasitic natural host ovum.
As a result as shown in Fig. 1,2,3,4,5, the results showed that three factors and its reciprocation all significantly affect each biology ginseng Number, wherein the influence with Storage period and temperature to development and breeding is maximum.Compared with the control, the queen bee ratio base in female generation and filial generation This is not influenced by cold deposit.Cold to deposit more than two weeks, eclosion rate will receive influence, to influence other biological parameters. It is deposited 1-3 weeks and prepupal period (96h) is cold at 13 DEG C deposits 1-4 weeks in addition to larval phase (48h) is cold at 10 DEG C, eclosion rate is all with storage It deposits the extension of phase and declines.Larval phase is cold at 4 DEG C and 7 DEG C to deposit, and eclosion rate, fecundity and egg-laying season all sharply decline and contract It is short, even without at bee eclosion.4 DEG C and 10 DEG C prepupal period is influenced it is maximum, but can be obtained at 13 DEG C with compare it is similar Breeding effect.Pupa time is seriously affected by low-temperature storage, can only be no more than 2 weeks in 13 DEG C of refrigerations.Although pupa advanced stage is in difference There is into bee eclosion after temperature storage, but it is significant with contrast difference.It screens 7 processing and compares constructed life table, it is clear Best storage worm state is prepupal period, and optimum refrigerated storage temperature is 13 DEG C, and longest Storage period is 4 weeks.
Embodiment 2:
Artificial eggs, egg of oak silkworm cultivate the low-temperature storage experimental method of trichogramma
With the trichogramma dendrolimi (Trichogramma dendrolimi) of artificial eggs liquid and egg of oak silkworm cultivation for object, Study influence of the low temperature stress to its growth and development.On the basis of not influencing numerous bee quality and application effect, artificial eggs are determined Most suitable storage temperature, worm state and the Storage period of bee and tussah egg parasite.
Three factors are arranged in this test, and 3 repetitions of every processing are respectively:
A. store worm state: this test with trichogramma 27 DEG C of optimum temperature with developed under conditions of relative humidity 75% when Long control worm state, from first oviposition to adult eclosion elapsed-time standards be 48h (small grub phase), 96h (prepupal period), 144h (pupa time) and 192h (pupa advanced stage).The confirmation development worm state under anatomical lens.
B. storage temperature: experimental design (4 ± 1) DEG C, (7 ± 1) DEG C, (10 ± 1) DEG C and (13 ± 1) DEG C 4 temperature.
C. storage time: with one week (7d) for a stage, in totally 4 stage, surrounding 28d is lasted.
Biological indicator and quality evaluation parameter:
From parasitic rate, percentage of pupation, eclosion rate, normal bee rate, female and male ratio, Life of Adult, generation time, reproductive capacity and its Trend index of population etc. investigates response of the In Vitro Rearing of Trichogramma to low temperature stress.
Artificial egg parasite experimental result (table 1)
The result shows that (Fig. 1-Fig. 5) three factors and its reciprocation all significantly affect each biological parameter, wherein with storage The influence of phase and temperature to development and breeding is maximum.Compared with the control, female queen bee ratio for filial generation is not substantially by cold The influence deposited.Cold to deposit more than two weeks, eclosion rate will receive influence, to influence other biological parameters.In addition to larval phase (48h) is cold at 10 DEG C to be deposited 1-3 weeks and prepupal period (96h) is cold at 13 DEG C deposits 1-4 weeks, and eclosion rate is all with the extension of Storage period And decline.Larval phase is cold at 4 DEG C and 7 DEG C to deposit, and eclosion rate, fecundity and egg-laying season all sharply decline and shorten, even without At bee eclosion.4 DEG C and 10 DEG C influence maximum to prepupal period, but can obtain at 13 DEG C and compare similar breeding effect.Pupa Phase is seriously affected by low-temperature storage, can only be no more than 2 weeks in 13 DEG C of refrigerations.Although pupa advanced stage different temperatures storage after all There is into bee eclosion, but it is significant with contrast difference.It screens 7 processing and compares constructed life table, specify best storage worm State is prepupal period, and optimum refrigerated storage temperature is 13 DEG C, and longest Storage period is 4 weeks.
Tussah egg parasite experimental result (table 1)
Compared with the control, female generation and the queen bee ratio of filial generation are not influenced by cold deposit substantially.It is cold to deposit more than two weeks, plumage Rate will receive influence, to influence other biological parameters.The Optimum storage phase is can to store at 4 DEG C in larval phase or pupa time 4 weeks, pupa time can store 4 weeks at 7 DEG C.It has been more than threshold of development under the conditions of 13 DEG C, each developmental stage stores up at 13 DEG C The bee out that sprouted wings in incubator more than 3-4 weeks is deposited, the temperature as low-temperature storage is not suitable for.Prepupal period is at 7 DEG C, pupa time It cannot all be stored more than 2 weeks at 10 DEG C.
Table 1

Claims (2)

1. a kind of method of In Vitro Rearing of Trichogramma low-temperature storage, which is characterized in that be to cultivate the artificial eggs liquid of prepupal period Trichogramma is stored.
2. the method for In Vitro Rearing of Trichogramma low-temperature storage according to claim 1, which is characterized in that be by prepupal period The trichogramma that artificial eggs liquid is cultivated is stored in 13 DEG C.
CN201810866607.6A 2018-08-01 2018-08-01 A kind of method of In Vitro Rearing of Trichogramma low-temperature storage Pending CN109156437A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116171941A (en) * 2023-03-08 2023-05-30 福建省农业科学院水稻研究所 Application of wheat seedlings in cold storage of trichogramma longsynanthassemblies and cold storage method
CN116235825A (en) * 2023-04-14 2023-06-09 广东省科学院动物研究所 Method for prolonging shelf life of egg parasitic wasps

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104255660A (en) * 2014-09-01 2015-01-07 吉林农业大学 Method for alternately storing trichogramma at intermediate and low temperature in two steps
CN108056070A (en) * 2018-01-16 2018-05-22 吉林农业大学 Utilize the method for white snow moths attracted by lamplight breeding trichogramma

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104255660A (en) * 2014-09-01 2015-01-07 吉林农业大学 Method for alternately storing trichogramma at intermediate and low temperature in two steps
CN108056070A (en) * 2018-01-16 2018-05-22 吉林农业大学 Utilize the method for white snow moths attracted by lamplight breeding trichogramma

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
马春森: ""Effects of constant temperature, exposure period, and age on diapause induction in Trichogramma dendrolimi"", 《BIOLOGICAL CONTROL》 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116171941A (en) * 2023-03-08 2023-05-30 福建省农业科学院水稻研究所 Application of wheat seedlings in cold storage of trichogramma longsynanthassemblies and cold storage method
CN116235825A (en) * 2023-04-14 2023-06-09 广东省科学院动物研究所 Method for prolonging shelf life of egg parasitic wasps

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Application publication date: 20190108