CN109220904A - The breeding of oriental cherry Thailand lion goldfish and selection - Google Patents
The breeding of oriental cherry Thailand lion goldfish and selection Download PDFInfo
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- CN109220904A CN109220904A CN201811130257.3A CN201811130257A CN109220904A CN 109220904 A CN109220904 A CN 109220904A CN 201811130257 A CN201811130257 A CN 201811130257A CN 109220904 A CN109220904 A CN 109220904A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The present invention provides breeding and the selection of a kind of oriental cherry Thailand lion goldfish, include the following steps Juvenile stage, parent fish rearing, artificial induced spawning, artificial insemination, artificial incubation, young spray cultivate, seed screening.The method of oriental cherry Thailand lion goldfish breeding and breeding of the present invention; the character that should have when detailing cultivation to the requirement of parent population and oriental cherry Thailand lion goldfish; targeted breeding and breeding are carried out, stabilization characteristics of genetics, the varietal characteristic of obtained oriental cherry Thailand lion goldfish are obvious, and purification degrees are high.
Description
Technical field
The invention belongs to goldfish to breed field, the breeding and breeding more particularly, to goldfish new varieties oriental cherry Thailand lion goldfish
Method.
Background technique
Oriental cherry Thailand lion goldfish is language goldfish, and it is not single that the formation of safe lion, which is not simply to cultivate, screen, yet
The breeding offspring of large meatball.Safe lion is the new varieties that large meatball goldfish native to China passes through that Thailand cultivates again, belongs to side
Depending on ornamental type, suitable glass cylinder, and the formation of safe lion are different according to the breeding practice in different Thailand fish farms and have very big differentiation,
Wherein it is mixed with the blood lineages such as pearl, gold-decorated, literary fish.Safe lion back is higher, and tail portion is more opened up, and in the head similar blue longevity, head accounts for entirety
One third to a quarter, color-variable, body colour is gorgeous, and side view is graceful.It is suitble to aquarium raising ornamental.Oriental cherry Thailand lion gold
Fish is a new varieties for safe lion, should have have a well filled-out figure, body drapes over one's shoulders soft squama, scale coating is uniform, tail fin is short and graceful, red
The features such as obvious with white boundary；Likewise, being made it have preferable ornamental to keep the gene of oriental cherry Thailand lion goldfish to stablize
Degree, needs to carry out stringent breeding and breeding.
Summary of the invention
The purpose of the present invention is the methods by reciprocal cross to spend safe lion and red safe lion as parent for five, cultivates base
Because of stable new varieties-oriental cherry Thailand lion goldfish, and provide breeding and the selection of above-mentioned oriental cherry Thailand lion goldfish, the method
Comprise the following steps: (1) Juvenile stage and cultivation: selecting the red safe lion goldfish of hard shell to make male parent, and the soft flower of squama five goldfish makees female parent, from
Autumn upper one year, a point male and female are put in a suitable place to breed, are alternately fed with artifical compound feed, and wherein breeding density is an age kind fish 8~9
Tail/m3, two age 4~5 tails of kind fish/m3；
(2) before and after mid-April, by water temperature stability at 22 DEG C or so, artificial induced spawning, process of hastening parturition artificial induced spawning: are carried out
In for an age kind fish, 2~4 tail milters are directly put into female fishpond, such as sees that milter chases female, then hastens parturition without hormone,
Directly carry out manually crowded ovum, insemination；For two ages and the above parent fish, every kilogram of raun injects 5 milliliters of oxytocins, every kilogram of hero
Fish injects 1 milliliter of oxytocins, then carries out manually squeezing ovum, insemination again；
(3) it artificial insemination: when obvious chasing behavior occurs in parent fish, fishes for parent fish and manually award under water
Essence, fish nest is put into be filled with water operates in basin, and right-hand man point holds each tail of male and female parent population, in underwater, male and female cloacal aperture is opposite,
The ovulation of raun abdomen is first squeezed with thumb, is squeezed milter abdomen sperm drainage immediately, is gently agitated for water body, it is made sufficiently to be fertilized, and
Uniform strength is attached on Hatching fish net；
(4) artificial incubation: keep in hatchery water everywhere dissolved oxygen >=7mg/L, deep 35~45 centimetres of water, launch fish
Ovum density is 1.5~1.8 ten thousand/m3；Fish-egg enters pond hatching and manually pulls the booties such as offscum out in every 6 hours, and measures water body items
Index, with mould inhibitor dipping, water is changed in full pond after twenty minutes within every 20~24 hours, until fish-egg is by membrane, in hatching process
Keep water temperature steady, timely sunshade and Jia Shui, and avoid changing that water temperature difference is more than 1.5 DEG C and water temperature is more than 30 DEG C；
(5) spray is cultivated: seed cultivation pool is doubled as with hatchery, after fish-egg sends out eye, is patrolled one time within every 6 hours, observation
Fish-egg survival rate and results of regular determination ensure that dissolved oxygen reaches 7mg/L to water everywhere, after observing the flat trip of fry membrane, take 2 within every 4 hours
~4 fry microexaminations determine that fry enteron aisle is unobstructed, after intraperitoneal yolk is exhausted, in time remove fish nest out of pond
And zooplankter bait is added into pond；
(6) seed is screened: it is divided into level-one screening and second level screening, level-one is screened the 26th~30 day after fry hatching, this
When fry body 1.5~2.5cm of overall length, tail fin point four leaves, diastole such as butterfly, fin spine are unobvious, travelling form is graceful, inferior without deformity
Fry retain, remaining is all eliminated, and the fry of reservation is carried out a point pond according to size and is raised, divide pond density 3~40,000 tails/
Pond, preventing excessive individual from sucking small individual tail fin causes inferior rate to rise；Second level is screened the 55th~60 day after fry hatching, this
When fry 2.5~4cm of overall length, have begun presentation color, and there is certain figure, this time screening need to be by cinerous, Quan Hong
Color, whole white and hard shell individual are chosen, and the fish chosen is gradable, classification is sold, and remaining juvenile fish should be raised again according to size point pond
It supports, is oriental cherry Thailand lion goldfish after dividing pond density 1~1.5 ten thousand tails/pond, juvenile fish to form.
Preferably, step (1) the parent culture stage, it was primary to change water weekly within the previous moon of hastening parturition, and produced the last week
It is interior, it is primary that water is changed every other day, and step up water temperature to 22 DEG C with GEOTHERMAL WATER, and gonad development is stimulated with water flow.
Preferably, the oxytocic hormone used in the step (2) is using physiological saline as solvent, and every milliliter contains 40 micrograms
DOM, LRH-A2 and 400 microgram HCG carry out one injection in parent's fish pectoral fin base portion, enter needle depth and be no more than 0.5 centimetre, with
Exempt to stab internal organ.
Preferably, first female male parent caught before artificial induced spawning in the step (2), checked sexual gland developmental state, gently
Milter abdomen is pressed, has white sperm to flow out, it is light that raun abdomen is pressed to have the outflow of ovum grain, mention raun caudal peduncle, hence it is evident that visible two sides ovum
The sagging profile of nest proves that gonad development of parents is good.
Preferably, hatching pool area is 12~15m in the step (4)2, hatchery water is natural phreatic water, and is used
Nylon silk cover filterings more than 200 mesh；Hatchery water body hydrostatic transparency 60cm or more, bottom of pond are increased using nanotube gas disk aeration
Oxygen, every pond are arranged 2, are located at the left and right sides in pond center.
Preferably, in the step (5) spray breeding phase fry density up to 1.6 ten thousand/m3More than, full water flower up to 8~
110000 tails.
Preferably, in the step (5) injected volume of zooplankter bait and release time according to fry density and
Fish body schistostoma size is matched, and guarantee is splashed the uniformity, and for bait based on the small-sized freshwater rotifer manually cultivated, yolk class is dynamic
Supplemented by physical property bait.
It is highly preferred that the small-sized freshwater rotifer is Branchionus urceus or Brachionus angularis or three limb wheel animalcules.
Preferably.In the seed screening process, mould proof, disinfection treatment is carried out after selecting every time, to reduce hand picking
When damage to fish body caused by lose.
It is highly preferred that selecting methyl blue to make mould inhibitor during the mould proof, disinfection treatment, povidone iodine makees disinfectant
The advantages and positive effects of the present invention are:
Due to the adoption of the above technical scheme, the oriental cherry Thailand lion goldfish that breeding of the invention and breeding method obtain, posture are rich
Full, body drapes over one's shoulders that soft squama, scale coating is uniform, tail fin is short and graceful, red obvious with white boundary, has stabilization characteristics of genetics, product
The advantages that kind feature is obvious, has a well filled-out figure, and gonad development is preferable.
Specific embodiment
Below and specific embodiment the invention will be further described.
The present invention spends safe lion and red safe lion as parent by the method for reciprocal cross using five, and it is stable to cultivate gene
New varieties-oriental cherry Thailand lion goldfish, the method for specific legal person breeding and breeding, include the following steps:
1.1 Juvenile stage
Selecting male parent is the red safe lion goldfish (♂) of hard shell, and female parent is soft squama five flower goldfish (♀)；Parent culture is from autumn the previous year
It is in season, the stabilization characteristics of genetics filtered out, varietal characteristic is obvious, it has a well filled-out figure, the preferable individual of gonad development is as parent
This.
1.2 parent culture
Parent culture was since autumn upper one year, the apparent healthy individuals of stabilization characteristics of genetics, varietal characteristic that filter out
Point male and female are put in a suitable place to breed, are replaced with artifical compound feed (predominantly plumule class) with the natural live bait such as cladocera and are fed；Breeding density is
One age 8~9 tails of kind fish/m3, two age 4~5 tails of kind fish/m3, produce in the previous moon, it is primary to change water weekly, produces in the last week, every other day
It is primary to change water, and steps up water temperature to 22 DEG C with GEOTHERMAL WATER, and gonad development is stimulated with water flow.
1.3 artificial induced spawning
Before and after mid-April, by water temperature stability at 22 DEG C or so, artificial induced spawning is carried out, first female male parent has been caught, has been checked
Gonad development situation (particular exam method are as follows: light pressure milter abdomen has white sperm to flow out, light that raun abdomen is pressed to have ovum grain stream
Out, raun caudal peduncle is mentioned, hence it is evident that the profile of visible two sides descensus ovarii, having features described above is the good parent of gonad development).
One age kind fish can hasten parturition without hormone, and 2~4 tail milters are put into female fishpond, such as see that milter chases female, then can directly carry out
Manually crowded ovum, insemination；Two ages and the above raun oxytocic hormone are solvent with physiological saline, and every milliliter contains 40 micrograms DOM, LRH-A2
And 400 microgram HCG, every kilogram of raun inject 5 milliliters of oxytocins, every kilogram of milter injects 1 milliliter of oxytocins, pectoral fin base portion into
Row one injection pays attention into 0.5 centimetre of needle depth or so, in order to avoid stab internal organ.
1.4 artificial insemination
It hastens parturition and parent population is put back into net cage or cement pit after injecting, when there is obvious chasing behavior after several hours, fishing
Parent population is taken to carry out underwater artificial insemination and be put into be filled with water by fish nest to operate in basin, right-hand man point holds each tail of male and female parent population, in the water surface
Under, male and female cloacal aperture is opposite, the ovulation of raun abdomen is first squeezed with thumb, is squeezed milter abdomen sperm drainage immediately, is gently agitated for
Water body makes it sufficiently be fertilized, and uniformly strength is attached on Hatching fish net that (specific method can be with referenced patent number for CN2728226's
Patent document).
1.5 artificial incubation
Hatching pool area is 12~15m2, hatchery water is natural phreatic water, and with nylon bolting silk mistakes more than 200 mesh
Filter prevents that harmful organisms enter；Hatchery water body hydrostatic transparency 60cm or more, bottom of pond use nanotube gas disk aeration oxygen replenishing,
Every pond is arranged 2, is located at the left and right sides in pond center, so that dissolved oxygen is all larger than 7mg/L, water deep 35~45 to water everywhere
Centimetre, dispensing fish-egg density is 1.5~1.8 ten thousand/m3(500g ovum is 400,000)；Fish-egg enters pond and hatches artificial fishing in every 6 hours
The booties such as offscum out, and water body indices are measured, with mould inhibitor dipping, water is changed in full pond after twenty minutes within every 20~24 hours, until
Fish-egg i.e. will be until membrane.Such as torrential rain, pond should be covered, avoid water quality, water temperature fluctuation excessively acute and influence hatching
Rate, keep in hatching process water temperature it is steady it is critically important answer timely sunshade and Jia Shui, avoid changing water temperature difference being more than that 1.5 DEG C and water temperature surpass
Cross 30 DEG C.
1.6 sprays are cultivated
Seed cultivation pool is doubled as with hatchery, hatching rate is up to 80%.After fish-egg sends out eye, patrols one time within every 6 hours, see
Examining fish-egg survival rate and results of regular determination ensures that dissolved oxygen reaches 7mg/L to water everywhere, if water is lower than the oxygen dissolving value, then should increase and receive
Mitron aeration plate quantity；After observing the flat trip of fry membrane, fry density is up to 1.6 ten thousand/m3More than, full water spends up to 8~
110000 tails.It takes 2~4 fry microexaminations within every 4 hours, determines that fry enteron aisle is unobstructed, after intraperitoneal yolk is exhausted, in time
Fish nest is added into zooplankter from removal in pond and into pond.
1.6.1 zooplankter bait selects
(it is sure not to make based on the small-sized freshwater rotifers such as Branchionus urceus, Brachionus angularis, the three limb wheel animalcules manually cultivated
The wheel animalcule inhabited with seawater is such as: brachionus plicatilis), when lacking bait, other animals can be selected according to fish body schistostoma size
Property bait (such as yolk)；Fry starts after ingesting, it is necessary to assure the animal bait splashed is uniform as far as possible, it is fresh and alive in this way can
It disposably feeds, for example frozen product should repeatedly be fed on a small quantity avoids aquatic environment from deteriorating；
1.6.2 zooplankter bait feeding amount
Determine fry hatching rate, ideally for hatching rate up to 60~80%, fry density is 1.75 ten thousand tails/m3, then
Recommend within 1st to feed wheel animalcule quantity to be about 8,000,000/m3Water body, it is about 9,600,000/m that second day, which feeds wheel animalcule quantity,3Water body,
Feeding wheel animalcule quantity third day is about 11,500,000/m3Water body can choose Tu Tang culture at this time or continue cement pit culture,
Bigger bait, such as cladocera, Copepods should be replaced in due course when cement pit culture according to fry schistostoma size；Feeding freezing,
The chilled every defective material of bait should ingest to finish in 1h and be advisable, and such as should suitably be reduced more than 1h and answer appropriate increment to feed lower than 1h；Tool
The every day feeding volume of body is adjusted in right amount with fry situation of ingesting.
The screening of 1.7 seeds
The screening of oriental cherry goldfish is divided into 2 times, for the first time after fry hatching at the 26th~30 day, fry body overall length at this time
1.5~2.5cm, four leaves of tail fin point, diastole such as butterfly, fin spine are unobvious, travelling form is graceful, retain without the inferior fry of deformity,
Remaining is all eliminated, and the fry of reservation is carried out a point pond according to size and is raised, and is divided pond density 3~40,000 tails/pond, is prevented excessive
Individual, which sucks small individual tail fin, causes inferior rate to rise, and screening retention rate for the first time should be 70% or more；Programmed screening is in fish
The 55th~60 day after seedling hatching, 2.5~4cm of fry overall length, has begun presentation color, and have certain figure at this time, this
Secondary screening need to choose cinerous, whole red, whole white and hard shell individual, and the fish chosen is gradable, classification sells, but cannot
Referred to as oriental cherry Thailand lion, remaining juvenile fish should be raised again according to size point pond, this screening retention rate should be in 65% or more, Fen Chimi
1~1.5 ten thousand tails/pond is spent, forming stage can be entered at this time.
The disinfectants such as mould inhibitors and povidone iodine such as methyl blue can be used to carry out mould proof, disinfection after selecting every time, to reduce
It is lost caused by damage when hand picking to fish body.
Embodiment 1:
Cultivation place is Tianjin Xiqing District company, (is detailed in the specific descriptions in 1.1~1.7) in aforementioned manners continuous 4
Year culture oriental cherry Thailand lion fry, hatching rate can reach 80% or more, reach as high as 90%；Farm's cement pit floor space
15m2, the depth of water is maintained at 40 ± 5cm during fry, then mean water volume is 6m3, highest oriental cherry Thailand lion spray density is reachable
1.65 ten thousand tails/m3, full pond cultivates 110,000 tail of spray or more, passes through 2 artificial screenings, qualified fry 4.5 ten thousand according to patented technology
It is more than tail, qualification rate 40.9%.
Above embodiments describe the invention in detail, but content is only the preferred embodiment of the present invention, no
It can be believed to be used to limit the scope of the invention.Any changes and modifications in accordance with the scope of the present application,
It should still fall within the scope of the patent of the present invention.
Claims (10)
1. breeding and the selection of oriental cherry Thailand lion goldfish, it is characterised in that: the method comprises the following steps: (1) parent selects
It selects and cultivates: the red safe lion goldfish of hard shell being selected to make male parent, the soft flower of squama five goldfish makees female parent, since autumn upper one year, divided male and female
It puts in a suitable place to breed, is alternately fed with artifical compound feed, wherein breeding density is age 8~9 tails of kind fish/m3, two age 4~5 tails of kind fish/m3；
(2) artificial induced spawning: before and after mid-April, by water temperature stability at 22 DEG C or so, carrying out artificial induced spawning, right during hastening parturition
Yu Yiling kind fish is directly put into 2~4 tail milters in female fishpond, such as sees that milter chases female, then hastens parturition without hormone, directly
Carry out manually crowded ovum, insemination；For two ages and the above parent fish, every kilogram of raun injects 5 milliliters of oxytocins, every kilogram of milter note
1 milliliter of oxytocins is penetrated, then carries out manually squeezing ovum, insemination again；
(3) artificial insemination: when obvious chasing behavior occurs in parent fish, fishing for parent fish and carry out underwater artificial insemination, will
Fish nest is put into be filled with water and operate in basin, and right-hand man point holds each tail of male and female parent population, in underwater, male and female cloacal aperture is opposite, with big
Thumb first squeezes the ovulation of raun abdomen, squeezes milter abdomen sperm drainage immediately, is gently agitated for water body, it is made sufficiently to be fertilized, and uniformly
Strength is attached on Hatching fish net；
(4) artificial incubation: keep in hatchery water everywhere dissolved oxygen >=7mg/L, deep 35~45 centimetres of water, it is close to launch fish-egg
Degree is 1.5~1.8 ten thousand/m3；Fish-egg enters pond hatching and manually pulls the booties such as offscum out in every 6 hours, and measures water body indices,
With mould inhibitor dipping, water is changed in full pond after twenty minutes within every 20~24 hours, until fish-egg is by membrane, keeps in hatching process
Water temperature is steady, timely sunshade and Jia Shui, and avoids changing that water temperature difference is more than 1.5 DEG C and water temperature is more than 30 DEG C；
(5) spray is cultivated: being doubled as seed cultivation pool with hatchery, after fish-egg sends out eye, is patrolled one time within every 6 hours, observe fish-egg
Survival rate and results of regular determination ensure that dissolved oxygen reaches 7mg/L to water everywhere, after observing the flat trip of fry membrane, take 2~4 within every 4 hours
Fry microexamination, determines that fry enteron aisle is unobstructed, after intraperitoneal yolk is exhausted, fish nest removed out of pond in time and to
Zooplankter bait is added in pond；
(6) seed is screened: being divided into level-one screening and second level screening, level-one is screened the 26th~30 day after fry hatching, at this time fish
Seedling body 1.5~2.5cm of overall length, four leaves of tail fin point, diastole such as butterfly, fin spine are unobvious, travelling form is graceful, without the inferior fish of deformity
Seedling retains, remaining is all eliminated, and the fry of reservation is carried out a point pond according to size and is raised, and divides pond density 3~40,000 tails/pond, prevents
Only excessive individual, which sucks small individual tail fin, causes inferior rate to rise；Second level is screened the 55th~60 day after fry hatching, at this time fish
Seedling 2.5~4cm of overall length, has begun presentation color, and has certain figure, and this time screening need to be by cinerous, whole red, complete
White and hard shell individual are chosen, and the fish chosen is gradable, classification is sold, and remaining juvenile fish should be raised again according to size point pond, point
Pond density 1~1.5 ten thousand tails/pond, juvenile fish form after be oriental cherry Thailand lion goldfish.
2. breeding and the selection of oriental cherry Thailand lion goldfish according to claim 1, it is characterised in that: the step (1)
The parent culture stage, it was primary to change water weekly within the previous moon of hastening parturition, and produces in the last week, it is primary to change water every other day, and use GEOTHERMAL WATER
Water temperature is stepped up to 22 DEG C, and gonad development is stimulated with water flow.
3. breeding and the selection of oriental cherry Thailand lion goldfish according to claim 1, it is characterised in that: the step (2)
The middle oxytocic hormone used is using physiological saline as solvent, and every milliliter contains 40 micrograms DOM, LRH-A2 and 400 microgram HCG, in parent
This fish pectoral fin base portion carries out one injection, enters needle depth no more than 0.5 centimetre, in order to avoid stab internal organ.
4. breeding and the selection of oriental cherry Thailand lion goldfish according to claim 1, it is characterised in that: the step (2)
In first female male parent caught before artificial induced spawning, check sexual gland developmental state, it is light to press milter abdomen, there is white sperm to flow out,
Light pressure raun abdomen has the outflow of ovum grain, mentions raun caudal peduncle, hence it is evident that the profile of visible two sides descensus ovarii proves parent's sexual gland hair
It educates good.
5. breeding and the selection of oriental cherry Thailand lion goldfish according to claim 1, it is characterised in that: the step (4)
Middle hatching pool area is 12~15m2, hatchery water is natural phreatic water, and with nylon silk cover filterings more than 200 mesh；It incubates
Change water body hydrostatic transparency 60cm or more, bottom of pond uses nanotube gas disk aeration oxygen replenishing, and every pond is arranged 2, is located at pond
The left and right sides in center.
6. breeding and the selection of oriental cherry Thailand lion goldfish according to claim 1, it is characterised in that: the step (5)
Middle spray breeding phase fry density is up to 1.6 ten thousand/m3More than, full water flower is up to 8~110,000 tails.
7. breeding and the selection of oriental cherry Thailand lion goldfish according to claim 1, it is characterised in that: the step (5)
The injected volume of middle zooplankter bait and release time are matched according to fry density and fish body schistostoma size, guarantee to sprinkle
Spill the uniformity, bait is based on the small-sized freshwater rotifer manually cultivated, supplemented by yolk class animal bait.
8. breeding and the selection of oriental cherry Thailand lion goldfish according to claim 7, it is characterised in that: the small-sized fresh water
Wheel animalcule is Branchionus urceus or Brachionus angularis or three limb wheel animalcules.
9. breeding and the selection of oriental cherry Thailand lion goldfish according to claim 1, it is characterised in that: the seed screening
In the process, mould proof, disinfection treatment, loss caused by damage when reducing hand picking to fish body are carried out after selecting every time.
10. breeding and the selection of oriental cherry Thailand lion goldfish according to claim 9, it is characterised in that: it is described it is mould proof, disappear
Methyl blue is selected to make mould inhibitor in malicious treatment process, povidone iodine makees disinfectant.
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| CN110269010A (en) * | 2019-07-29 | 2019-09-24 | 天津农学院 | The method of oriental cherry gold-plating goldfish germplasm initiative |
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